KR20180105354A - Microorganism Kit-based Porous Concrete Block for Odor Removal and Water Quality Improvement in Lake and River - Google Patents

Abstract

Disclose is a microbial kit-based porous concrete block for odor removal and water quality improvement in lake and river. More specifically, provided is a microbial kit-based porous concrete block for odor removal and water quality improvement in lake and river, into which a microbial kit is inserted. According to the present invention, it is possible to improve water quality and remove odor in lake or river by inserting a kit containing expanded vermiculite in which microbes are planted in the concrete block at regular intervals, while ensuring easy replacement of the kit. To this end, in the porous concrete block for odor removal and water quality improvement in lake and river, a microbial vegetation part is provided on a surface of the porous concrete block, and a microbe-planted porous material is included in the microbial vegetation part.

Description

[0001] The present invention relates to a microbial kit-based porous concrete block for odor removal and water quality improvement in a lake,

The present invention relates to a microbial kit-based porous concrete block for eliminating offensive odors and improving water quality in a lake and a river, and more particularly, to a porous concrete block having microbial kits that contain expanded vermiculite immobilized microbes The present invention relates to a porous concrete block based on microbial kits for removing odors and improving the quality of wastewater or rivers.

Conventional methods for microbial utilization for odor removal and water quality improvement in lake and river were simple spraying in the form of powder or active liquid. In this method, microorganisms are lost when the flow rate of the stream or lake is increased or when the flow rate is increased due to rainfall. Thus, the effect of the malodor removal and water quality improvement can not be expected or is very small.

In addition, a conventional technique for applying microorganisms to a porous concrete block replaces a certain amount of formulation water as a microbial culture liquid during the concrete compounding process. This method is based on the change of the hard alkaline environment (pH 11 ~ 12) due to hydration heat (over 40 ℃) and hydration product (Ca (OH) 2) due to concrete hardening, microbial growth pore And the reduction of organic nutrients (including moisture), most microorganisms are killed. Therefore, simple introduction of microorganisms into the porous concrete block can not be expected to reduce odor and improve water quality by the microorganisms after the installation of the porous concrete block.

Korean Patent No. 10-1490878 discloses a method of manufacturing and forming a biocontainer artificial cancer using an environmentally-friendly porous lightweight bio-ceramics stone. The present invention provides a concrete capable of attaching microorganisms and plants by producing concrete using porous ceramic stones. However, when used in a polluted environment, it may become a habitat for contaminants after the microorganisms attached thereto are killed Rather, it has the potential to exacerbate water quality.

Korean Patent No. 10-1156746 discloses a porous concrete composition utilizing a composite useful microorganism. In this invention, concrete is produced by using porous pumice as a breeding space of microorganisms. However, when microbes in the porous body are killed after the porous block is installed, it is difficult to further improve water quality.

Disclosure of the Invention The present invention has been conceived in order to solve the problems of the prior art described above, and it is an object of the present invention to provide a method of inserting a kit comprising expanded vermiculite having microorganisms immobilized at a previous interval in a concrete block, And it is an object of the present invention to provide a microbial kit-based porous concrete block for odor removal and water quality improvement in a lake and river which can be easily replaced with a new microbial kit even if the microbes die after installation.

As means for solving the above-mentioned technical problem,

The present invention relates to a porous concrete block for removing bad odors and improving the quality of water in rivers and streams, wherein a microbial vegetation part is present on the surface of the porous concrete block, and a microbial kit comprising a porous material in which microbes are planted is inserted into the microbial vegetation part A microbial kit-based porous concrete block for odor removal and water quality improvement in a lake and a river.

Also, the microorganism is Rhodocyclus gelatinosus , Rhodocyclus tenuis , Rhodobacter capsulatus And Rhodopseudomonas The present invention also provides a porous concrete block characterized by containing at least one microorganism selected from the group consisting of rutila .

Also, the porous material has a cation exchange capacity.

Also, the porous material is charcoal, pumice, diatomaceous earth, expanded vermiculite or zeolite.

Also, the microbial kit has a porous concrete block having a net-like structure on its surface.

According to the present invention, microorganisms adsorbed on a porous material are contained inside a microorganism kit having a net-like structure, and the microorganism kit is installed on the surface of the porous contryp block, It is possible to prevent the microorganisms from being killed and to easily remove the microorganism kit when the microorganisms are killed and a new kind of microorganism is needed after the installation, so that it is useful for long-term water purification and contaminant removal in various environments.

1 is a schematic view of a microbial kit-based porous concrete block according to an embodiment of the present invention,
2 is a schematic view of a porous concrete block according to an embodiment of the present invention,
3 is a schematic view of a microbial kit according to an embodiment of the present invention,
Fig. 4 is a photograph of a sample taken in the present invention. Fig. 4 (a) is a Sowokcheon sewage treatment plant, (b) is a shallow shallow stream, ,
Figure 5 is a schematic diagram of a streak plate method used in an embodiment of the present invention,
FIG. 6 is a graph showing an experiment result of water purification according to an embodiment of the present invention. FIG.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly explain the present invention, parts not related to the description are omitted, and similar parts are denoted by similar reference numerals throughout the specification.

FIG. 1 is an installation example of a microbial kit-based porous concrete block according to an embodiment of the present invention, and FIG. 2 is a plan view and a sectional view of a concrete block. 3 is a schematic view of a microbial kit installed in the concrete block.

Referring to FIG. 1, a microbial vegetation part is present on the surface of a porous concrete block, and a microbial kit including a porous material in which microbes are planted is inserted into the microbial vegetation part.

The porous concrete may have microbial vegetation on its surface. The porous concrete used in the present invention can be used without restriction on any commercially available porous concrete. In the present invention, unlike conventional microbial-based porous concrete, microorganisms and a porous concrete structure are separately manufactured, so that the porous concrete can be used regardless of the material. However, since charcoal, pumice, diatomaceous earth, expanded vermiculite, or zeolite having a cation exchange capacity is used as the microbial adsorbent of the present invention, it is possible to provide an environment similar to the microbial kit part, It is preferable to use a porous concrete containing vermiculite or zeolite.

The porous concrete may be prepared by mixing cement, fine aggregate, and water. In this case, 1 part by weight of cement; And 2 to 5 parts by weight of a fine aggregate containing 10 to 20% by weight of a porous material, and mixing the resulting mixture with water. The porous material may include charcoal, pumice, diatomaceous earth, expanded vermiculite or zeolite, and the content of water is preferably 0.2 to 0.3 part by weight with respect to 1 part by weight of cimant.

The cement includes Portland cement, mixed cement, special cement and the like. The Portland cement includes one kind of ordinary Portland cement, two kinds of heat Portland cement, three kinds of crude steel Portland cement, four kinds of low heat Portland cement, Portland cement, and the like, and the mixed cement includes a blast furnace slag cement, a portland pozzolan cement, a portland fly-ash cement, a color cement, and the like (KS L 5216), masonry cement (KS L5219), an agate steel (KS L5219), and a hardened steel cement Cement, and the like, but is not limited thereto.

Also, microbial vegetation may be present on the surface of the porous concrete. The microorganism vegetation part is formed in the same shape as the microorganism kit for inserting the used microorganism kit and can be formed into a shape such as a cylinder, a hexahedron, a triangular column, a pentagonal column, a hexagonal column, , The density of the vegetation part, and the like. It is preferable that the volume of the microorganism vegetation portion is 30% or less of the amount of cement used to secure the strength of the porous concrete. If the vegetation part is installed at a volume exceeding 30%, the strength of the porous concrete may be lowered and the porous concrete may be damaged at the time of installation or use.

The microorganism vegetation part may be provided with a microorganism kit. The microorganism kit is filled with a porous material on which microorganisms are adsorbed. The surface of the microorganism kit has a net structure to prevent the porous material from coming off and to facilitate contact with the surrounding environment. The net-like structure can be used without limitation as long as it prevents dropping of the porous material contained therein.

The porous material is a part where microorganisms are planted to remove odors and improve water quality. It is preferable to use a porous material having a cation exchange capacity on the surface for smooth feeding of the microorganisms cultured. Since the porous material having cation exchange ability has a good quality to adhere the organic materials, not only the nutrients can be continuously supplied to the microorganisms adsorbed on the porous material, but also when the microorganism kit is removed, a large amount of adsorbed organic materials can be removed Therefore, it can play a large role in eliminating odor and improving water quality.

The porous material may be any porous material having cation exchange ability. However, it is preferable to use charcoal, pumice, diatomaceous earth, expanded vermiculite or zeolite, and most preferably expanded vermiculite. This expanded vermiculite is a porous material capable of absorbing organic nutrients (media components) required for microbial and microbial growth. Since the pH is 5.5 to 7.5, it is the most ideal material for the optimum environment for growth of microorganisms. On the other hand, porous perlite and cork are not suitable for microbial sorbents because of their lack of cation exchange capacity. In addition, it is preferable that the porous material is made to have a diameter of 5 mm or less and is easily dispersed when immersed in water while increasing its surface area.

In the present invention, the porous material includes Rhodocyclus gelatinosus , Rhodocyclus tenuis , Rhodobacter capsulatus And Rhodopseudomonas it is preferable that one or more microorganisms selected from the group of rutila are planted. The microorganisms capable of decomposing the phosphorus and nitrogen components in the water are planted in the porous material to decompose the phosphorus and nitrogen components to thereby remove odors and improve water quality. Therefore, any microorganism that can be used for this purpose can be planted without limitation, but Rhodocyclus gelatinosus , Rhodocyclus tenuis , Rhodobacter capsulatus And Rhodopseudomonas rutila may be planted. It is also possible that one or more of the above-mentioned microorganisms are mixed and planted depending on the degree of pollution and the degree of pollutants used. In addition, the microorganisms are present only in the porous material at the time of planting, but they may diffuse into the porous concrete around the microbial kit and grow when the condition of the surrounding environment is suitable, thereby allowing the microorganisms cultured in the whole porous concrete to be inhabited. In addition, since the microbial kit of the present invention can be easily replaced, when the environment is changed after the introduction of the first microorganism and the introduction of new microorganisms is required, new microorganisms can be easily injected by exchanging the kit with the new microorganisms.

Hereinafter, the present invention will be described in detail with reference to examples.

Example  One

(1) Microbial sample collection and water quality analysis

(A) Sampling

Samples were collected from Suwon Reservoir in Suwon City, Gyeonggi Province, and Sowokcheon, one of the Daecheong Reservoir in Okcheon County, Chungcheongbuk - do. Before the sampling, the water bottle was washed, and the sample was floated so as to mix well and collected. Samples were collected from 10 surface waters and one sediment. Each sample was carried in an ice box at 4 ° C (FIG. 4).

(B) Ammonia analysis

Ammonia (NH3-N, 0 to 2.50 mg / L) was measured using DR2010 (HACH, USA). 25 ml of distilled water was added to a 25 ml cell and used as a blank. The collected sample was measured using raw water. Mineral

Three drops of stabilizer were dropped and invert mixed. Next, 3 drops of polyvinyl alcohol were dropped and invert mixed. Then, 1 ml of Nessler reagent was added, and after invert mixing, reacted for 1 minute and ammonia was measured based on the blank.

(C) Nitrate analysis

Nitrate (NO3 - N, 0 to 30.0 mg / L) was measured using DR2010 (HACH, USA). Add 25 ml of sample to a 25 ml cell and measure the initial value. Next, add Nitraver 5 nitrate reagent powder and mix well for 1 minute. The reaction was carried out for the next 5 minutes, and the nitrate was measured by measuring the latter value.

(D) Nitrite analysis

Nitrite (NO2 - N, 0 to 0.300 mg / L) was measured using DR2010 (HACH, USA). Add 10 ml of sample to 10 ml of cell, and measure the initial value. Next, add NitriVer 3 Nitrite reagent and dissolve well. The reaction was continued for the next 20 minutes, and nitrite was measured by measuring the latter value.

(E) Phosphate analysis

Phosphate (PO43-, 0.00 to 5.00 mg / L) was measured using DR2010 (HACH, USA). Reactive Phosphorus Test 'N tube Dilution Add 5 ml of the sample to the vial and measure the initial value. Then add PhosVer3 Phosphate Powder Pillow and mix well. After 2 minutes of reaction, phosphate was measured by measuring the latter value.

(F) pH analysis

After the pH meter was calibrated, the pH was measured while thoroughly mixing the collected samples.

(2) Preparation of selective medium

In this experiment, nutrient medium and selective medium were used to isolate strains from small oxacillin samples. Nutrient broth was used as a nutrient medium (Table 1) and Nitrosomonas medium (Table 2), Nitrobacter medium (Table 3) and Actinomycete growth medium (Table 4) were used as selection media based on reports of previous documents. In addition, carbon dioxide gas was injected into the inorganic medium (Table 5) in order to secure a bacterium using an independent nutrient source. Nitrogen gas was injected into the 27S medium (Table 6) and the modified 27M (DSM) (Table 7) and Actinomycete growth medium for securing photosynthetic bacteria using an independent nutrient source under anaerobic conditions.

Beef extract 3 g Peptone 5 g Distilled water 1 L

₍₄ NH) ₂ SO ₄ 2 g MgSO ₄ .7H ₂ O 200 mg CaCl ₂ .2H ₂ O 20 mg KH ₂ PO ₄ 15.9 mg Ferric citrate 0.5 mg Na ₂ MoO ₄ .2H ₂ O 100 [mu] g CoCl ₂ .6H ₂ O 2 [mu] g CuSO ₂ .5H ₂ O 20 [mu] g ZnSO ₄ .7H ₂ O 100 [mu] g NaCl 500 mg KHCO ₃ 20 mg MnCl ₂ .4H ₂ O 200 [mu] g Distilled water 1 L pH 7.8-8.0

KNO ₃ 300 mg MgSO ₄ .7H ₂ O 0.2 g CaCl ₂ .2H ₂ O 0.02 g KH ₂ PO ₄ 500 mg K ₂ HPO ₄ 500 mg FeCl ₄ .7H ₂ O 0.01 g NaCl 187.5 mg KHCO ₃ 1500 mg Distilled water 1 L pH 7.5 to 8.0

Succinic acid 1.18 g L-Glutamine 0.29 g CaCl ₂ .2H ₂ O 0.2 g KH ₂ PO ₄ 0.2 g MgSO ₄ .7H ₂ O 0.2 g NaCl 0.1 g m-Inositol 0.09 g Ferric EDTA 0.037 g MnSO ₄ .H ₂ O 4.5 mg H ₃ BO ₃ 1.5 mg ZnSO ₄ .7H ₂ O 1.5 mg Nicotinic acid 0.5 mg Pyridoxine-HCl 0.5 mg Thiamine-HCl 0.1 mg CuSO ₄ · 5H ₂ O 0.04 mg NaMoO ₄ · 2H ₂ O 0.025 mg Distilled water 1 L pH 6.4 ± 0.2

KH ₂ PO ₄ 2.0 g K ₂ HPO ₄ 2.0 g KNO ₃ 1.0 g ₍₄ NH) ₂ SO ₄ 2.0 g NaCl 0.4 g MgSO ₄ .7H ₂ O 0.2 g CaCl ₂ .2H ₂ O 0.02 g FeCl ₃ .7H ₂ O 0.01 g Trace element solution SL-6 ^a 1.0 ml VA vitamin solution ^b 1.0 ml Distilled water 1 L

Yeast extract 1.0 g Isodium succinate hexahydrate 1.0 g Absolute ethanol 0.5 ml Ferric citrate solution (0.5%) 1.0 ml KH ₂ PO ₄ 0.5 g MgSO ₄ .7H ₂ O 0.4 g NaCl 0.4 g NH ₄ Cl 0.4 g CaCl ₂ .2H ₂ O 0.05 g Trace element solution SL-6 1.0 ml Distilled water 1 L pH 6.8

Yeast extract 1.0 g Disodium succinate hexahydrate 1.0 g Absolute ethanol 0.5 ml Ferric citrate solution (0.5%) 1.0 ml KH ₂ PO ₄ 0.5 g MgSO ₄ .7H ₂ O 0.4 g NaCl 0.4 g NH ₄ Cl 0.4 g CaCl ₂ .2H ₂ O 0.05 g Trace element solution SL-6 ^a 1.0 ml Distilled water 1 L pH 6.8

(3) Cultivation and pure separation of microorganisms

(A) Growth culture

In the aerobic condition, 200 ml of nutrient medium and selective medium were put into a 500 ml bottle, and the samples were inoculated with 1 ~ 2% of the sample, followed by shaking at 150 rpm. In the anaerobic condition, 50 mL of 27S medium, 27M (DSM), and Actinomycete growth medium were added to 100 mL bottles, and nitrogen gas was injected for 5 minutes. One to two percent of the samples were inoculated After that, the irradiance was cultured at 2,000 lux. All cultures were performed at 29 ± 1 ° C.

(B) Pure separation

Strain plate method and millipore filter method were used for the pure separation of strains. For the pure separation, 1.5% agar plates were prepared as described above.

(a) Streak plate method

The mixed bacteria obtained from the proliferating cultures were separated pure on a 1.5% agar plate using a streak-method. The proliferated cultures were sputtered on a 1.5% agar plate as shown in Fig. 5 and cultured at 29 ± 1 ° C for 1 day. After culturing, a colony formed on the plate was identified, picked using a sterilized toothpick, and inoculated into each nutrient medium and selective medium. Nitrosomonas medium, Nitrobacter medium, Actinomycete growth medium and inorganic medium were cultured under aerobic conditions, 27S medium, 27M (DSM) and Actinomycete growth medium were cultured under anaerobic conditions. The anaerobic culture was used in the case of the liquid culture medium after filling the culture medium with a rotary stopper bottle or a test tube and injecting nitrogen gas. When the solid culture medium was used, it was deeply cultured in a test tube. The plate media was incubated in a gas pack anaerobic jar (gas pack anaerobic system, BBL). For aerobic culture, 500 ml of 200 ml of culture solution was added to the liquid culture medium, and the culture was inoculated with a lid, followed by shaking culture. The photosynthetic bacteria were incubated in 60W incandescent lamps and fluorescent lamps in two rows.

(b) Millipore filter method

The collected samples were purified by a millipore filter method. The sample was filtered through a 0.2 [micro] l mixed cellulose ester membrane filter, and the membrane filter was placed on a 1.5% agar nutrient medium for 1 day. When a colony was formed on the membrane filter, the bacteria were separated using a sterilized toothpick. After separation, pure separation was confirmed through a streak. The pure separation was observed at 1,000-fold using an optical microscope (Olympus BH-2).

(4) Characterization and identification of strains

(A) Morphological characteristic

The morphology of the strains was observed at 1000-fold magnification using an optical microscope (Olympus BH-2) and photographs were taken at 1,000 magnifications using an Olympus optical microscope (C-35 DA-2). When observing mobility and cleavage patterns, they were observed in a non-fixed state on the phase difference.

In addition, the isolated strains were identified as Gram-positive / negative strains by Gram staining (X1000, BX50 Olympus, Japan). Gram stain was stained with crystal violet for 2 minutes and 30 seconds, fixed with iodine for 1 minute, decolorized with 20% acetone ethanol, and stained with safranine for 2 minutes and 30 seconds.

(B) Physiological characteristic

The highly efficient strains were identified by using the API 20E kit (Bio Merieux) used for the identification of Gram-negative bacteria in the intestine and the API 20NE kit (Bio Merieux) used for identification of non-Gram-negative bacteria. The physiological and biochemical characteristics Respectively. It was identified through the apiweb ™ database.

(C) 16S rDNA analysis

The strains to be analyzed were streaked on Nutrient agar and cultured at 28 ° C for 24 hours. 0.5 ml of sterilized distilled water was dispensed into 1.5 ml E-tube, and the target strain was platinum and suspended. After heating at 100 ° C for 5 minutes, the supernatant was separated by centrifugation at 12,000 rpm for 30 seconds using a Microcentrifuge (Micro-13, Korea). The separated supernatant was used as a template for PCR, and the composition was as shown in Table 8 below.

Reaction solution Conc. Final conc. Vol. for PCR (占 퐇) 10Xmg ²⁺ buffer 20mM 2.0 mM 2.0 dNTP 2.5 mM 0.2 mM 1.6 Primer 27F 10 pmole 1.0 1492R 1.0 Taq polymerase 5unit / μl 1 unit 0.2 Template 1.0 H2O 13.2 Total vol. 20.0

PCR reaction was performed according to the above composition. The PCR reaction was performed using a PCR thermal cycler dice (Takara, Japan). The reaction conditions are shown in Table 9 below.

Reaction step Temp. (° C) Time (sec) Pre-denaturation 95 180 Denaturation 95 30
30 cycles Annealing 60 30 Polymerization 72 90 Last extension 72 600 Storage 4 ∞

The PCR reaction was purified using a PCR purification kit (Intron, Korea), loaded with 5 μl of each in 1.0% agarose gel (1XTAE buffer), and subjected to electrophoresis (Mupid-2plus, Takara) Respectively.

In order to identify the PCR reaction product by DNA sequencing, a solicited nucleotide sequence analysis was requested by Solgent Co., and the analyzed nucleotide sequence was blasted at NCBI site (Http://www.NCBI.nlm.nih.gov) Blast searching.

(5) Separation and proliferation of microorganisms for odor removal and water quality improvement

The strains were grown in nutrient broth and selective medium. As a result, 98 strains were obtained from 48 strains in nutrient medium, 32 strains in Nitrosomonas medium and 18 strains in Nitrobacter medium.

Among the strains isolated from Daecheong Lake, 14 strains isolated from 27M (DMS) medium were 13, 19, 35, 38, 43, 47, 53, 54, 58, 61, 62, 66, The results of the identification are as follows.

As a result, strain 13 was identified as Rhodocyclus gelatinosus, strain 19 as Rhodocyclus tenuis, strain 66 as Rhodobacter capsulatus, strains 53 and 61 as Rhodopseudomonas rutila. Among the unidentified strains, strains 3 and 77 had ovoid morphology. The carbon source requirement was close to that of Rhodopseudomonas blastica, but there were many differences. Strains 43 and 47 were ovate and were similar to Rhodopseudomonas sulforviridis. The strains 54 and 62 have bacterium morphology, and the results of the carbon source experiments were close to Rhodocyclus gelatinus or Rhodopseudomonas rutila, but there were many differences. The resolution of each isolated strain is shown in Table 10 below.

division 13 19 35 38 43 47 53 54 58 61 62 66 75 77 Citrate + - - - - - - + + - - - - - Thiosulfate - - - - - - - + + - - - + + Manitol - - + - - - - + + - - - + + Glucose + + + + + + + + + + - + + + Fructose + - - - + - + + + + + + + + Propionate - - - - + - - - + - - + + - Malate + - - - - - + + + + + + + + Acetate + - + - + - + + + + + + + + Lactate + + - - + - + + + + + + - + Sorbitol - - + + - - + + + + - - + + Glycerol + + - - - - + + + + + - + + Gluconate + + - - - - + + + + - - + + Arginine - - - - - + - + + - - - + + Fumarate + + + + + + + + + + + + + + Tartrate - - - - - - - + + - - - + + Carproate - - - - - - + + + - - - + - Aspartate + - + + + + - + + - + + + + Valelic acid (+) + - - - - + + + + + - + + Pyruvate + + + + + + + + + + + + + + Benzoate - - - - - - - + - - - - + - Carprylate - - - - - - - + - - - - + - Formate - - - - - - - + + - - - + - Malonate - - - - - - + + - + - - + - Butyrate (+) - - - - - - ND + - - + ND +

(6) Nitrogen Removability  Selection of strains

A screen test was conducted to select strains with nitrogen removal ability, and 28 strains were carried out in 4 steps. Ammonia concentration was adjusted to 20ppm and 40ppm with NH4Cl reagent in the medium to select strains with ammonia removal ability during nitrogen removal ability.

The screen test was conducted four times, and the results are shown in the following table. As a preliminary experiment, the results shown in Table 11 were obtained using actual wastewater. Table 12 shows the results of ammonia removal rate when CODcr is 20 ppm, ammonia 20 ppm and total phosphorus concentration is 1 ppm, and Table 13 shows the results obtained when CODcr is 20 ppm, ammonia 20 ppm, and total phosphorus 5 ppm.

Strain No. 0 h 20 h Rem. (%) KN 2 22.0 (0.0) 16.5 (+ - 3.1) 24.8 KN 6 22.0 (0.0) 17.8 (+/- 3.0) 19.0 KN  22 22.0 (0.0) 2.7 (+ - 0.2) 87.8 KN 37 22.0 (0.0) 4.1 (± 0.1) 81.4

Strain No. 0 h 20 h Rem. (%) One 22.3 (+/- 2.4) 21.1 (+ - 0.2) 5.5 3 21.5 (+/- 0.1) 19.5 (+ - 0.9) 9.1 5 22.3 (+ - 0.2) 19.6 (+ - 0.3) 6.2 7 22.3 (+ - 0.2) 20.3 (+/- 0.8) 3.1 8 22.3 (+ - 0.2) 20.2 (+/- 1.6) 3.3 9 21.5 (+/- 0.1) 18.8 (+/- 0.1) 12.3 10 22.3 (+ - 0.2) 20.3 (+ - 0.2) 3.1 11 21.5 (+/- 0.1) 19.4 (+/- 1.1) 9.8 12 21.5 (+/- 0.1) 20.4 (0.0) 5.1 13 22.3 (+/- 2.4) 20.5 (+ - 0.2) 8.2 14 22.3 (+ - 0.2) 19.0 (+ - 1.7) 9.3 16 21.5 (+/- 0.1) 19.6 (+/- 0.1) 8.8 17 22.3 (+/- 2.4) 19.8 (+/- 1.2) 11.3 19 22.3 (+ - 0.2) 19.8 (0.7) 5.3 22 22.3 (+ - 0.2) 19.2 (+/- 1.6) 8.1 23 21.5 (+/- 0.1) 21.9 (± 0.3) - 24 22.3 (+ - 0.2) 20.7 (+/- 0.5) 1.2 25 22.3 (+ - 0.2) 19.5 (+ - 0.2) 6.7 26 21.5 (+/- 0.1) 21.0 (+ - 0.2) 2.3 27 22.3 (+/- 2.4) 19.8 (+/- 1.4) 11.1 28 21.5 (+/- 0.1) 20.4 (+/- 2.1) 5.1 29 21.5 (+/- 0.1) 21.1 (+ - 0.6) 1.6 30 22.3 (+ - 0.2) 21.9 (+/- 1.1) - 13-1 21.5 (+/- 0.1) 21.7 (+/- 2.1) -

Strain No. 0 h 20 h Rem. (%) DC 1 22.7 (+/- 0.3) 21.5 (0.0) 5.3 DC 2 22.7 (+/- 0.3) 20.2 (+/- 0.5) 10.9 DC 3 22.7 (+/- 0.3) 22.4 (+/- 0.1) 1.0 DC 4 22.7 (+/- 0.3) 21.1 (+ - 0.7) 6.8 DC 5 22.7 (+/- 0.3) 20.5 (± 0.3) 9.4 DC 6 22.7 (+/- 0.3) 22.4 (0.0) 1.0 DC 7 22.7 (+/- 0.3) 22.1 (0.0) 2.6 DC 8 22.7 (+/- 0.3) 22.0 (+/- 0.1) 3.1 DC 9 22.7 (+/- 0.3) 21.6 (+/- 1.3) 4.5 DC 10 22.7 (+/- 0.3) 22.4 (+/- 0.5) 1.1 DC 11 22.7 (+/- 0.3) 20.7 (± 0.3) 8.7 DC 12 22.7 (+/- 0.3) 19.7 (+/- 0.3) 13.1 DC 13 22.7 (+/- 0.3) 18.4 (+ - 0.3) 18.9 DC 14 22.7 (+/- 0.3) 20.3 (+ - 0.6) 10.6 DC 15 22.7 (+/- 0.3) 22.1 (+/- 0.1) 2.6 DC 16 22.7 (+/- 0.3) 19.9 (+/- 0.1) 12.1 DC 17 22.7 (+/- 0.3) 15.4 (+ - 9.0) 32.0 DC 18 22.7 (+/- 0.3) 19.8 (+/- 1.2) 12.6 DC 19 22.7 (+/- 0.3) 21.4 (+/- 0.6) 5.6 DC 20 22.7 (+/- 0.3) 21.9 (+/- 0.8) 3.3 DC 21 22.7 (+/- 0.3) 19.3 (0.0) 14.8 DC 22 22.7 (+/- 0.3) 19.5 (+ - 0.7) 14.0 DC 23 22.7 (+/- 0.3) 20.7 (+/- 2.2) 8.9 DC 24 22.7 (+/- 0.3) 20.1 (+ - 0.2) 11.1 DC 25 22.7 (+/- 0.3) 19.9 (+ - 0.2) 12.0 DC 26 22.7 (+/- 0.3) 22.3 (0.0) 1.5 DC 27 22.7 (+/- 0.3) 21.5 (+ - 0.2) 5.2 DC 28 22.7 (+/- 0.3) 22.8 (+/- 0.1) -0.8 DC 29 22.7 (+/- 0.3) 20.8 (+/- 1.4) 8.3 DC 30 22.7 (+/- 0.3) 21.1 (+ - 0.7) 7.1

(7) Selection of strains having phosphorus removal ability

Screening tests were carried out to screen strains with the ability to remove phosphorus, and 23 strains were performed in 4 runs. In order to select strains with total elimination ability, total phosphorus concentration was set to 1 ppm and 5 ppm with KHPO reagent in medium. The screen test was carried out four times, and all the screen tests measured the total phosphorus. The results are shown in Table 14 below.

Strain No. 0 h 20 h Rem. (%) One 5.4 (0.0) 2.8 (+/- 1.8) 48.4 3 5.6 (0.0) 4.9 (0.0) 11.5 5 5.1 (± 0.0) 4.7 (± 0.1) 8.7 7 5.1 (± 0.0) 5.0 (± 0.2) 2.7 8 5.1 (± 0.0) 5.7 (+/- 0.8) - 9 5.6 (0.0) 4.8 (0.0) 14.9 10 5.1 (± 0.0) 4.7 (± 0.1) 8 11 5.6 (0.0) 5.6 (+/- 0.1) - 12 5.6 (0.0) 5.5 (+ - 0.2) 1.4 13 5.4 (0.0) 4.3 (± 1.7) 20.9 13 5.6 (0.0) 5.9 (0.0) - 14 5.1 (± 0.0) 4.9 (+/- 0.1) 5.4 16 5.6 (0.0) 5.1 (+/- 0.4) 9.4 17 5.4 (0.0) 3.6 (+/- 2.0) 32.8 19 5.1 (± 0.0) 4.7 (+/- 1.0) 8.5 22 5.1 (± 0.0) 4.7 (± 0.1) 7.6 23 5.6 (0.0) 5.9 (+/- 0.1) - 24 5.1 (± 0.0) 4.9 (+/- 0.3) 3.3 25 5.1 (± 0.0) 4.6 (+ - 0.2) 9.1 26 5.6 (0.0) 5.1 (± 0.1) 8.2 27 5.4 (0.0) 4.8 (+/- 0.1) 11 28 5.6 (0.0) 5.3 (± 0.0) 6 29 5.6 (0.0) 4.8 (+/- 0.1) 14.2

Example  2

Experiments were conducted using the microorganisms (strains) isolated in Example 1 above. The microorganism was sufficiently planted in the expanded vermiculite having a diameter of 5 mm or less, and then the normally-expanded expanded vermiculite was placed in a microbial kit having a size of 100 X 50 X 50 mm. Then, the water quality purification experiment was carried out using the porous concrete block with 100 microbial kits.

Comparative Example  One

Microbial - based porous concrete blocks were fabricated in the same manner as the conventional method. The culture broth containing the same amount of microorganisms as those used in Example 2 was mixed with water and replaced with a part of the gravel by the same amount as the amount of expanded vermiculite used in Example 2 .

Experimental Example

The water purification test was carried out using the above-described Example 2 and Comparative Example 1. [

division Example 2 Comparative Example 1 Ammonia
(NH ₃ -N) Phosphorus
(PO ₄ -P ³⁾ COD Ammonia
(NH ₃ -N) Phosphorus
(PO ₄ -P ³⁾ COD Inflow 3.55 1.73 7.66 3.55 1.73 7.66 Outflow 0.74 0.61 2.1 2.41 0.88 4.21 Rem (%) 79.1 64.5 72.6 31.2 48.9 44.4

As shown in FIG. 6, in the case of Example 2 of the present invention, the concentration of ammonia in the effluent could be significantly lowered. As shown in Table 15, phosphorus, nitrogen and COD removal efficiencies were significantly improved compared to conventional microbial-based porous concrete, which suggests that the addition of microbial kits after the microbial death would increase the difference do.

The preferred embodiments of the present invention have been described in detail with reference to the drawings. It will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the present invention as defined by the appended claims.

Therefore, the scope of the present invention is defined by the appended claims rather than the foregoing detailed description, and all changes or modifications derived from the meaning, range, and equivalence of the claims are included in the scope of the present invention. .

Claims (5)

A porous concrete block for odor removal and water quality improvement in a lake and a river,
There is a microbial vegetation on the surface of the porous concrete block,
Wherein the microbial vegetation part is provided with a microbial kit containing a porous material in which microbes are planted. The microbial kit-based porous concrete block for removing odors and improving water quality in a lake and a river. The method according to claim 1,
Microorganisms are Rhodocyclus gelatinosus , Rhodocyclus tenuis , Rhodobacter capsulatus and Rhodopseudomonas lt; RTI ID = 0.0 > 1, < / RTI > rutila . The method according to claim 1,
Wherein the porous material has a cation exchange capacity. The method of claim 3,
Wherein the porous material is charcoal, pumice, diatomaceous earth, expanded vermiculite or zeolite. The method according to claim 1,
Wherein the microbial kit has a net-like structure on its surface.

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