KR20180058356A - Carbonic anhydrase supprot, method for preparing the same, apparatus for separating carbon dioxide, and method for separating carbon dioxide using the same - Google Patents

Abstract

A carbonic anhydrase support of the present invention comprises: a porous boehmite; and a carbonic anhydrase and magnetic nanoparticles supported in the porous boehmite with a bonding agent. The present invention provides the carbonic anhydrase support capable of preventing loss during operation and having excellent durability and activity, a manufacturing method thereof, an apparatus for separating carbon dioxide, and a method for separating the carbon dioxide using the same.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a carbonic anhydrase stabilizer, a method for producing the carbonic anhydrase, a method for producing the carbonic anhydrase, a method for producing the carbonic anhydrase, a method for producing the carbonic anhydrase,

The present invention relates to a carbonic anhydrase stabilizer, a method for producing the same, a carbon dioxide separator, and a method for separating carbon dioxide using the same.

Recently, there is growing international interest in how to curb emissions of greenhouse gases, which are the cause of global warming. Particularly, efforts to reduce carbon dioxide, which is an acid gas in the greenhouse gas, have been made worldwide. In order to reduce the amount of carbon dioxide emitted from industrial sites, a process for converting carbon dioxide into high-value-added compounds is being developed, as well as technologies for capturing and storing carbon dioxide. Conventionally, abatement techniques based on physical and chemical methods have been developed or studied, and in recent years, attempts have been made to apply them to processes for capturing or converting carbon dioxide using enzymes obtained from natural sources.

However, carbonic anhydrase has a low activity and durability due to the nature of the enzyme in spite of its excellent ability to absorb carbon dioxide, so that it can be used for a long time And it is lost during operation, which makes it difficult to continuously separate carbon dioxide.

Accordingly, there is a need for an apparatus and a method for removing carbon dioxide, which is excellent in activity and durability and is capable of preventing the loss of oil, and is applied to a carbonic anhydrase.

Prior art related to this is disclosed in Korean Patent Publication No. 1991-0018075.

An object of the present invention is to provide a carbonic anhydrase stabilizer capable of preventing loss during operation, a method for producing the same, a carbon dioxide separator, and a method for separating carbon dioxide using the same.

Another object of the present invention is to provide a carbonic anhydrase stabilizer having excellent durability and activity, a method for producing the same, a method for separating carbon dioxide, and a method for separating carbon dioxide using the same.

The above and other objects of the present invention can be achieved by the present invention described below.

One aspect of the present invention relates to a carbonic anhydrase stabilizer.

According to one embodiment, the carbonic anhydrase stabilizer comprises a porous boehmite, and a carbonic anhydrase and a magnetic nanoparticle supported on the porous boehmite by a binding agent.

The carbonic anhydrase stabilizer may include 0.001 to 1 part by weight of carbonic anhydrase, 0.001 to 2 parts by weight of magnetic nanoparticles, and 1 to 10 parts by weight of a binder, based on 100 parts by weight of the porous boehmite.

The magnetic nanoparticles can include _{Fe 2 O 3, Fe 3 O} 4, CoFe 2 O 4, MnFe 2 O 4, FePO 4, Fe (PO 4) 2, one or more of Fe and Fe ₃ C.

The binder may include at least one of polyethylene glycol (PEG), polyvinyl alcohol, polystyrene, and polyaniline.

Another aspect of the present invention relates to a method for producing a carbonic anhydrase inhibitor.

In one embodiment, the method for producing a hydrogelatin hydrogel polymeric solid preparation comprises mixing aqueous aluminum nitrate (Al (NO) 39H2O) and aqueous ammonia (NH4OH) to prepare an aqueous solution of boehmite; And adding a carbonic anhydrase, a magnetic nanoparticle and a binder to the boehmite aqueous solution to prepare a mixed solution.

The method for producing the carbonic anhydrase inhibitor comprises mixing 40 to 60% by weight of an aluminum nitrate aqueous solution (Al (NO) ₃ 9H ₂ O) from 10 mM to 50 mM and 40 to 60% by weight of ammonia water (NH ₄ OH) Thereby preparing an aqueous solution of boehmite; And 0.001 to 1 part by weight of carbonic anhydrase, 0.001 to 2 parts by weight of magnetic nanoparticles and 1 to 10 parts by weight of a binder are added to 100 parts by weight of the boehmite aqueous solution to prepare a mixed solution.

The method for preparing a carbonic anhydrase inhibitor may further comprise adjusting the pH of the composition for forming a hydrogelatinase fixed body to 7.0 to 9.0.

Another aspect of the present invention relates to a carbon dioxide separation apparatus.

In one embodiment, the carbon dioxide separation device comprises a housing for containing an absorption liquid, a filling part formed at the end of the housing, the filling part including the carbonic anhydrase fixing body, a gas supply part for introducing a carbon dioxide- And a gas discharging part formed on the filling part and discharging the gas from which carbon dioxide has been removed.

The carbonic anhydrase fixative may be impregnated into the absorbent.

The filling part includes a magnet, a carbonic anhydrase fixing body magnetically attached on the magnet, and a top flange and a bottom flange for capturing the magnet, and the top flange and the bottom flange may be formed of a metal mesh network.

In another embodiment, the carbon dioxide separation device includes a pH measurement unit formed in the absorption liquid, an absorption liquid discharge unit discharging the absorption liquid when the pH value measured by the pH measurement unit is not more than a predetermined value, And an absorption liquid supply unit for supplying the absorption liquid.

In another embodiment of the present invention, the carbon dioxide separation device includes a first valve provided in the absorption liquid discharge unit and controlling the discharge amount of the absorption liquid in accordance with the pH value measured by the pH measurement unit, and a second valve provided in the absorption liquid supply unit, And a second valve for controlling the supply amount of the absorption liquid according to the second valve.

Another aspect of the present invention relates to a carbon dioxide separation process.

In one embodiment, the carbon dioxide separation method may comprise hydrating carbon dioxide from the carbon dioxide-containing gas into the absorption liquid using the carbonic anhydrase stabilizer.

The present invention has the effect of providing a carbonic anhydrase stabilizer capable of preventing loss during operation and having excellent durability and activity, a method for producing the same, a carbon dioxide separator, and a method for separating carbon dioxide using the same.

FIG. 1 schematically shows a carbon dioxide separation apparatus according to one embodiment of the present invention.
2 schematically shows a carbon dioxide separation apparatus according to another embodiment of the present invention.
FIG. 3 is a graph showing the results of measurement of activity of carbonic anhydrase for 90 days in Example 3. FIG.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Reference will now be made in detail to embodiments of the present application, examples of which are illustrated in the accompanying drawings. However, the techniques disclosed in this application are not limited to the embodiments described herein but may be embodied in other forms.

Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of this invention to those skilled in the art. In the drawings, the width, thickness, and the like of the components are enlarged in order to clearly illustrate the components of each device. In addition, although only a part of the components is shown for convenience of explanation, those skilled in the art can easily grasp the rest of the components.

The terms "upper" and "lower" in this specification are defined with reference to the drawings, Quot; or "on" may include not only superimposition but also interposition of another structure in the middle. On the other hand, what is referred to as "directly on" or "directly above"

Although the terms including ordinals such as first, second, etc. in this specification can be used to describe various elements, the elements are not limited by the terms. The terms are used only for the purpose of distinguishing one component from another.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention. In the drawings, the same reference numerals denote substantially the same elements.

It is to be understood that the singular forms "a", "an", and "the" include plural referents unless the context clearly dictates otherwise, and the terms "comprise" That does not preclude the presence or addition of one or more other features, integers, steps, operations, elements, parts, or combinations thereof, .

Further, in carrying out the method or the manufacturing method, the respective steps of the method may take place differently from the stated order unless clearly specified in the context. That is, each process may occur in the same order as described, may be performed substantially concurrently, or may be performed in the opposite order.

Hereinafter, the present invention will be described in detail.

Carbonic anhydrase Fixed body

According to one embodiment of the present invention, the carbonic anhydrase stabilizer includes a porous boehmite, a carbonic anhydrase and a magnetic nanoparticle supported on the porous boehmite by a binding agent.

The carbonic anhydrase can accelerate the rate of hydration of carbon dioxide up to about 100 to 10 million folds, so that the carbon dioxide separation efficiency is excellent and the separated carbon dioxide can be easily recycled. Specifically, when injected carbon dioxide to the water containing the carbonic anhydrase, and carbon dioxide is separated in the form of carbonate ions (CO ₃ ^2-), it can be recycled in various ways carbonate ion (CO ₃ ^2-). For example, calcium carbonate (CaCO ₃ ) can be prepared by injecting a cation such as Ca ^{2 +} into the carbonate ion (CO ₃ ^2- ).

The carbonic anhydrase can be extracted from, but not limited to, bovine serum, human serum, or oyster, such as oyster.

The carbonic anhydrase stabilizer may contain 0.001 to 1 part by weight, specifically 0.001 to 0.1 part by weight, more specifically 0.005 to 0.025 part by weight, of the carbonic anhydrase according to 100 parts by weight of the porous boehmite. In the above content range, not only the rate of hydration of carbon dioxide can be sufficiently accelerated, but also the durability of the carbonic anhydrase stabilizer is excellent.

The porous boehmite is an alumina hydrate, and serves as a substrate for fixing the carbonic anhydrase. Specifically, the porous boehmite has a large contact area with the carbon dioxide and the absorption liquid, so that the carbon dioxide hydration reaction rate can be further increased. Further, the application of boehmite which is excellent in durability and is environmentally friendly has an advantage that the activity of carbonic anhydrase can be maintained without loss of the carbonic anhydrase stabilizer even if it is used for a long time in the carbon dioxide separation process.

The magnetic nanoparticles can impart magnetism to the carbonic anhydrase stabilizer. The magnetically fixed carbonic anhydrase stabilizer can be trapped and fixed by a magnet, and thus it is advantageous to prevent loss of fluid even in flowing fluid. The magnetic nanoparticles may be bound to the porous boehmite by a binder.

The magnetic nanoparticles may include at least one of Fe ₂ O ₃ , Fe ₃ O ₄ , CoFe ₂ O ₄ , MnFe ₂ O ₄ , FePO ₄ , Fe (PO ₄ ) ₂ , Fe and Fe ₃ C, And is not limited as long as it can impart magnetism to the anhydrase.

The carbonic anhydrase stabilizer may contain 0.001 to 2 parts by weight, specifically 0.01 to 1 part by weight, more specifically 0.04 to 0.2 part by weight, of the magnetic nanoparticles based on 100 parts by weight of the porous boehmite. In the above content range, the carbonic anhydrase stabilizer can have sufficient magnetic properties and is also excellent in durability.

The binding agent may play a role in forming a carbonic anhydrase stabilizer by increasing the binding force between the porous boehmite, the carbonic anhydrase and the magnetic nanoparticles. And may include at least one of the above polyethylene glycol (PEG), polyvinyl alcohol, polystyrene, and polyaniline.

The carbonic anhydrase stabilizer may contain the binder in an amount of 1 to 10 parts by weight, specifically 1 to 5 parts by weight, based on 100 parts by weight of the porous boehmite. In the above content range, the carbonic anhydrase stabilizer has a sufficient binding force between the components and is excellent in durability.

Carbonic anhydrase Fixed body  Manufacturing method

According to one embodiment of the present invention, the method for producing a carbonic anhydrase inhibitor comprises mixing aluminum nitrate (Al (NO) ₃ 9H ₂ O) and aqueous ammonia (NH ₄ OH) Preparing an aqueous solution of sodium hypochlorite; Adding a carbonic anhydrase, a magnetic nanoparticle and a binder to the boehmite aqueous solution to prepare a mixed solution; And solidifying the mixed solution.

The chopping to prepare a boehmite solution is 10mM to aluminum nitrate aqueous solution of _{50mM (Al (NO) 3 9H} 2 O) 40 to 60% by weight, in particular aqueous ammonia of 45 to 55% by weight of 30mM to 150mM (NH ₄ OH) By mixing 40 to 60 wt%, specifically 45 to 55 wt%, and stirring at 500 rpm for 2 hours at 15 DEG C to 35 DEG C, to prepare an aqueous solution of boehmite. In the above content range, not only the surface area of the porous boehmite is sufficiently wide, but also the durability is excellent.

The boehmite aqueous solution may be subjected to a step of washing the impurities with water and ethanol, and the washing step may be performed one to five times.

The step of adding the carbonic anhydrase, the magnetic nanoparticles, and the binder to prepare a mixed solution comprises adding 0.001 to 1 part by weight, more preferably 0.005 to 0.025 part by weight, of carbonic anhydrase to 100 parts by weight of the boehmite aqueous solution, To 2 parts by weight, specifically 0.04 to 0.2 part by weight, and the binder 1 to 10 parts by weight, specifically 1 to 5 parts by weight.

The prepared carbonic anhydrase stabilizer can be stored at 0 캜 to 10 캜 to maintain the enzyme activity.

The method for preparing a carbonic anhydrase inhibitor may further comprise adjusting the pH of the composition for forming a hydrogelatinase fixed body to 7.0 to 9.0. For the pH adjustment, a buffer solution may be used. For example, the buffer solution may be a tris-HCl buffer solution, but is not limited thereto.

Carbon dioxide separator

Hereinafter, a carbon dioxide separation apparatus according to one embodiment of the present invention will be described with reference to FIG. FIG. 1 schematically shows a carbon dioxide separation apparatus according to one embodiment of the present invention.

In one embodiment, the carbon dioxide separator comprises a housing 1 for receiving the absorbent 100, a filler 200 formed at the end of the housing 1 and including the carbonic anhydrase fixture 210, A gas supply unit 300 through which the carbon dioxide-containing gas flows into the lower part of the filling unit 200 and a gas discharge unit 400 through which the gas from which the carbon dioxide is removed is formed on the filling unit 200 have.

The housing 1 is not limited as long as it can accommodate the absorbing liquid. The housing 1 may, for example, use acrylic.

The absorption liquid 100 can be separated by dissolving carbon dioxide from the carbon dioxide-containing gas flowing into the carbon dioxide separator. The absorption liquid 100 may be water, or water containing an inorganic substance, but is not limited thereto.

The filling part 200 includes a magnet 230 and a carbonic anhydrase fixing body magnetically attached on the magnet; And an upper flange 251 and a lower flange 253 for capturing the magnet 230. The upper flange 251 and the lower flange 253 may be formed of metal mesh meshes.

The filling part 200 captures the upper flange 251 and the lower flange 253 with the magnet 230 attached with the carbonic anhydrase fixing body 210 so that carbon dioxide is absorbed from the carbon dioxide- And it is possible to prevent the carbonic anhydride enzyme fixing body 210 from being lost even when the carbon dioxide separator is operated for a long time.

The type of the magnet 230 is not limited as long as the magnet 230 serves to fix the carbonic anhydrase fixing body 210 by a magnetic force. The shape of the magnet 230 may be spherical, rod-like, or plate-like, but is not limited thereto. In order to increase the contact probability with the carbon dioxide-containing gas without disturbing the flow of the carbon dioxide-containing gas, a spherical magnet can be applied. In order to stably fix the carbonic anhydrase stabilizer to the magnet, the diameter of the magnet may be 5 mm to 20 mm, specifically 10 mm to 15 mm.

The upper flange 251 and the lower flange 253 on the filling part 200 are easy to detach and attach from the carbon dioxide separating device and the filling part can be separated separately according to the situation to easily produce the carbonic anhydrase There is an advantage that the fixture can be replaced.

The upper flange 251 and the lower flange 253 may include a metal mesh network for smooth flow of the carbon dioxide-containing gas flowing into the carbon dioxide separation device and free flow of the absorption liquid 100. The metal mesh net can smoothly flow the carbon dioxide-containing gas and the absorption liquid, and functions to capture the magnet 230. The metal mesh net may have a lattice spacing of 1 mm to 5 mm, specifically 2 mm to 3 mm.

The carbonic anhydrase fixture 210 may be impregnated with the absorbent 100 to hydrate carbon dioxide from the carbon dioxide-containing gas into the absorbent 100.

The gas supplying unit 300 may be located below the filling unit 200 to allow the carbon dioxide-containing gas to pass through the filling unit 200. The pressure of the carbon dioxide-containing gas flowing into the gas supply unit 300 may be in the form of a tube, but the present invention is not limited thereto. The pressure of the carbon dioxide-containing gas may be determined by considering the scale of the carbon dioxide separation unit, the carbon dioxide concentration of the absorption liquid, and the amount of carbonic anhydrase in the filling unit 200 .

The gas discharge unit 400 may be located on the top of the filling unit 200, for example, on the side of the top of the filling unit 200 or on the top of the carbon dioxide separation unit. In FIG. 1, the gas discharging unit 400 is positioned above the carbon dioxide separating apparatus and the upper portion of the carbon dioxide separating apparatus housing is formed into a conical shape for ease of discharging. However, the present invention is not limited thereto.

Hereinafter, a carbon dioxide separation apparatus according to another embodiment of the present invention will be described with reference to FIG. 2 schematically shows a carbon dioxide separation apparatus according to another embodiment of the present invention.

In another embodiment, the carbon dioxide separation device includes a pH measurement unit 500 formed in the absorption liquid 100, an absorption liquid discharge unit 500 for discharging the absorption liquid 100 when the pH value measured by the pH measurement unit 500 is equal to or less than a predetermined value, And an absorption liquid supply unit 700 for supplying the same amount of the absorption liquid as the discharged absorption liquid.

The pH measurement unit 500 is formed in the absorption liquid 100 and can measure the pH of the absorption liquid 100 to calculate the carbon dioxide content in the absorption liquid 100. When the carbon dioxide content is high, the pH is decreased. When the carbon dioxide content is below a certain pH, the absorption efficiency of the absorption liquid 100 may be deteriorated. Therefore, when the pH value measured by the pH measuring unit 500 is lower than a predetermined value, the absorbing solution 100 is discharged through the absorbing solution discharging unit 600, and the same amount of absorbing solution as the discharged absorbing solution is discharged from the absorbing solution supplying unit 700 The carbon dioxide separation (or removal) efficiency of the carbon dioxide separation device can be maintained by appropriately maintaining the pH of the absorption liquid 100 supplied and supplied. The predetermined pH value may be 5, for example, taking into consideration the carbon dioxide solubility of the absorbing solution 100. However, the pH value may be appropriately adjusted according to the circumstances.

The absorbing liquid discharged to the absorbing liquid discharging unit 600 contains carbon dioxide at a high concentration, which can be recycled by various methods. For example, calcium carbonate (CaCO ₃ ) may be produced by implanting a cation such as Ca ^{2 +} , but is not limited thereto.

The absorption liquid discharge portion 600 and the absorption liquid supply portion 700 may be in the form of a tube, but are not limited thereto.

In another embodiment, the carbon dioxide separation device includes a first valve (not shown) provided in the absorption liquid discharge unit 600 and controlling the amount of absorption liquid to be discharged according to the pH value measured by the pH measurement unit 500, And a second valve (not shown) provided in the absorbent solution supply unit 700 and controlling the supply amount of the absorbing solution according to the absorbent solution discharge amount.

Although not shown, the carbon dioxide separation device may further include a control unit for receiving the measured pH value from the pH measuring unit 500 and controlling the opening and closing of the first valve and the second valve when the measured value is equal to or less than a predetermined value have.

The carbon dioxide separation method according to another aspect of the present invention may include the step of hydrating carbon dioxide from the carbon dioxide-containing gas into the absorption liquid by using the carbonic anhydrase stabilizer.

Hereinafter, the configuration and operation of the present invention will be described in more detail with reference to preferred embodiments of the present invention. It is to be understood, however, that the same is by way of illustration and example only and is not to be construed in a limiting sense.

The contents not described here are sufficiently technically inferior to those skilled in the art, and a description thereof will be omitted.

Example  One: Carbonic anhydrase Fixed body  Produce

50% by weight of 60 mM ammonia water (NH ₄ OH) was added to 50% by weight of a 20 mM aluminum nitrate aqueous solution (Al (NO) ₃ .9H ₂ O) and stirred at 25 ° C and 500 rpm for 2 hours to obtain an aqueous boehmite solution. And ethanol three times or more to remove impurities. 0.1 part by weight of magnetic nanoparticles, 2.5 parts by weight of polyethylene glycol (PEG) and 0.017 part by weight of carbonic anhydrase (a carbonic anhydrase extracted from serum of bovine serum) were added to each 100 parts by weight of aqueous solution of boehmite, To pH 8.0 to prepare a carbonic anhydrase stabilizer and stored at 4 ° C.

Example  2

As shown in FIG. 2, a housing having a size of 2,000 cc of acrylate is manufactured, and a filling part 200, a gas supplying part 300, a gas discharging part 400, a pH measuring part 500, A carbon dioxide separation device having a discharge part 600 and an absorption liquid supply part 700 was manufactured.

In the filling part 200, 50 g of a magnet having an average particle diameter of 7 mm and 1 g of a carbonic anhydrase fixing material prepared in Example 1 were filled.

It was confirmed that the pH of the aqueous solution was decreased at an initial 7.2 by measuring the pH change of the aqueous solution while continuously supplying the mixed gas of 15% carbon dioxide and 85% nitrogen to the gas supply part 300 at a rate of 10 ml / min. When the pH was 5 or less, the carbon dioxide separation process was continued for 90 days by discharging the absorption liquid to the absorption liquid discharge unit 600 and supplying a new absorption liquid from the absorption liquid supply unit 700 by the discharged amount.

After 90 days, the pH of the absorption liquid was lowered to 5 or less, and the absorption liquid having a high carbon dioxide content was discharged to the absorption liquid discharge unit 600, thereby confirming that the carbon dioxide hydration reaction was continued.

Example  3

The activity of the carbonic anhydrase stabilizer was measured at intervals of 10 days for 90 days during the carbon dioxide separation process in Example 2, and the relative activity (based on the initial activity of 100%) is shown in FIG.

How to measure activity

The activity changes of the carbonic anhydrase stabilizers were measured by hydrolysis of 4-NPA (4-nitrophenylacetate). Carbonic anhydrase has the property of hydrolyzing 4-NPA with 4-nitrophenol and acetic acid, and it turns yellow. The indirect method of measuring 4-nitrophenol (UV) was used.

As shown in FIG. 3, the carbonic anhydrase stabilizer of the present invention includes magnetic nanoparticles and an environmentally friendly and durable porous boehmite to minimize the loss of carbonic anhydrase during the carbon dioxide separation process, It can be seen that 90% or more of the initial activity is maintained.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be understood that the invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. It is therefore to be understood that the embodiments described above are in all respects illustrative and not restrictive.

100: absorption liquid 200: filling part
210: Carbonic anhydrase 230: Magnet
251: Upper flange 253: Lower flange
300: gas supply part 400: gas discharge part
500: pH measuring unit 600: absorbent discharge unit
700: Absorbent solution supply part

Claims (13)

Porous boehmite; And
A carbonic anhydrase and a magnetic nanoparticle carried on the porous boehmite as a binding agent;
≪ / RTI >
The method according to claim 1,
The above-mentioned carbonic anhydrase inhibitor,
With respect to 100 parts by weight of the porous boehmite,
0.001 to 1 part by weight of carbonic anhydrase,
0.001 to 2 parts by weight of magnetic nanoparticles, and
1 to 10 parts by weight of a binding agent.
The method according to claim 1,
Carbonic anhydrase that the magnetic nanoparticles includes _{Fe 2 O 3, Fe 3 O} 4, CoFe 2 O 4, MnFe 2 O 4, FePO 4, Fe (PO 4) 2, one or more of Fe and Fe ₃ C Fixing.
The method according to claim 1,
Wherein the binder comprises at least one of polyethylene glycol (PEG), polyvinyl alcohol, polystyrene, and polyaniline.
Preparing an aqueous solution of boehmite by mixing aqueous aluminum nitrate solution (Al (NO) ₃ 9H ₂ O) and aqueous ammonia (NH ₄ OH); And
Adding a carbonic anhydrase, a magnetic nanoparticle and a binder to the boehmite aqueous solution to prepare a mixed solution;
≪ / RTI >
6. The method of claim 5,
40 to 60 wt% of a 10 mM to 50 mM aqueous aluminum nitrate solution (Al (NO) ₃ 9H ₂ O) and 40 to 60 wt% of aqueous ammonia (NH ₄ OH) of 30 mM to 150 mM to prepare an aqueous solution of boehmite; And
Adding 0.001 to 1 part by weight of carbonic anhydrase, 0.001 to 2 parts by weight of magnetic nanoparticles and 1 to 10 parts by weight of a binder to 100 parts by weight of the boehmite aqueous solution to prepare a mixed solution;
≪ / RTI >
6. The method of claim 5,
Further comprising the step of adjusting the pH of the composition for forming a carbonic anhydrase immobilization body to 7.0 to 9.0.
A housing for receiving an absorbent;
A filling part formed at the end of the housing and including the carbonic anhydrase fixture of any one of claims 1 to 7;
A gas supply unit for introducing a carbon dioxide-containing gas into the lower portion of the filling unit; And
A gas discharge unit formed on the filling unit and discharging the gas from which the carbon dioxide is removed;
And a carbon dioxide separator.
9. The method of claim 8,
Wherein the carbonic anhydrase stabilizer is impregnated into the absorption liquid.
9. The method of claim 8,
Wherein the filling portion comprises: a magnet;
A carbonic anhydrase fixture magnetically attached on the magnet; And
An upper flange and a lower flange for capturing the magnet,
Wherein the upper flange and the lower flange are formed of a metal mesh net.
9. The method of claim 8,
A pH measurement unit formed in the absorption liquid;
An absorbent outlet for discharging the absorbent when the pH measured by the pH measuring unit is less than a predetermined value; And
An absorption liquid supply unit for supplying the same amount of the absorption liquid as the discharged absorption liquid;
The carbon dioxide separation device further comprising:
12. The method of claim 11,
A first valve provided in the absorption liquid discharge unit for controlling the amount of absorption liquid discharged according to the pH value measured by the pH measurement unit; And
A second valve provided in the absorption liquid supply unit and controlling the supply amount of the absorption liquid in accordance with the absorption liquid discharge amount;
Further comprising:
A method for separating carbon dioxide, comprising the step of hydrating carbon dioxide from a carbon dioxide-containing gas into an absorption liquid using the carbonic anhydrase stabilizer of any one of claims 1 to 7.

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