KR20180055033A - Method for preparing chungkookchang comprising nipa fruticans wurmb and chungkookchang by the method - Google Patents
Method for preparing chungkookchang comprising nipa fruticans wurmb and chungkookchang by the method Download PDFInfo
- Publication number
- KR20180055033A KR20180055033A KR1020160152312A KR20160152312A KR20180055033A KR 20180055033 A KR20180055033 A KR 20180055033A KR 1020160152312 A KR1020160152312 A KR 1020160152312A KR 20160152312 A KR20160152312 A KR 20160152312A KR 20180055033 A KR20180055033 A KR 20180055033A
- Authority
- KR
- South Korea
- Prior art keywords
- extract
- powder
- palm
- soybeans
- weight
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 22
- 244000004005 Nypa fruticans Species 0.000 title abstract description 11
- 235000005305 Nypa fruticans Nutrition 0.000 title abstract description 11
- 239000000284 extract Substances 0.000 claims abstract description 51
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 46
- 244000068988 Glycine max Species 0.000 claims abstract description 46
- 239000000843 powder Substances 0.000 claims abstract description 41
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000007654 immersion Methods 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 241001505935 Phalaenopsis Species 0.000 claims description 16
- 239000002994 raw material Substances 0.000 claims description 12
- 244000020518 Carthamus tinctorius Species 0.000 claims description 10
- 235000003255 Carthamus tinctorius Nutrition 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 244000304393 Phlox paniculata Species 0.000 claims description 7
- 210000003296 saliva Anatomy 0.000 claims description 7
- 244000063299 Bacillus subtilis Species 0.000 claims description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 claims description 3
- 235000013527 bean curd Nutrition 0.000 claims 1
- 238000000227 grinding Methods 0.000 claims 1
- 230000003078 antioxidant effect Effects 0.000 abstract description 17
- 241000193830 Bacillus <bacterium> Species 0.000 abstract description 9
- 239000004615 ingredient Substances 0.000 abstract description 3
- 238000002791 soaking Methods 0.000 abstract description 3
- 229940023462 paste product Drugs 0.000 abstract 3
- 235000005135 Micromeria juliana Nutrition 0.000 abstract 1
- 240000002114 Satureja hortensis Species 0.000 abstract 1
- 235000007315 Satureja hortensis Nutrition 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 21
- 230000000052 comparative effect Effects 0.000 description 10
- 239000000796 flavoring agent Substances 0.000 description 10
- 235000019634 flavors Nutrition 0.000 description 10
- 235000019645 odor Nutrition 0.000 description 10
- 230000002292 Radical scavenging effect Effects 0.000 description 9
- 238000000855 fermentation Methods 0.000 description 9
- 230000004151 fermentation Effects 0.000 description 9
- 238000005259 measurement Methods 0.000 description 9
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 7
- 230000001953 sensory effect Effects 0.000 description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 241000607184 Nipponia Species 0.000 description 4
- 235000002789 Panax ginseng Nutrition 0.000 description 4
- 240000000851 Vaccinium corymbosum Species 0.000 description 4
- 235000003095 Vaccinium corymbosum Nutrition 0.000 description 4
- 235000017537 Vaccinium myrtillus Nutrition 0.000 description 4
- 239000003963 antioxidant agent Substances 0.000 description 4
- 235000006708 antioxidants Nutrition 0.000 description 4
- 235000021014 blueberries Nutrition 0.000 description 4
- 238000010298 pulverizing process Methods 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 3
- 239000004480 active ingredient Substances 0.000 description 3
- 229910021529 ammonia Inorganic materials 0.000 description 3
- 238000009835 boiling Methods 0.000 description 3
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000021107 fermented food Nutrition 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 238000007602 hot air drying Methods 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 150000008442 polyphenolic compounds Chemical class 0.000 description 3
- 235000013824 polyphenols Nutrition 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 229930182490 saponin Natural products 0.000 description 3
- 150000007949 saponins Chemical class 0.000 description 3
- 238000003809 water extraction Methods 0.000 description 3
- 241000233788 Arecaceae Species 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 235000007516 Chrysanthemum Nutrition 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 2
- 238000007605 air drying Methods 0.000 description 2
- 235000003704 aspartic acid Nutrition 0.000 description 2
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 108010083364 chungkookjang Proteins 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000007598 dipping method Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
- 235000017173 flavonoids Nutrition 0.000 description 2
- 150000002215 flavonoids Chemical class 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 235000017709 saponins Nutrition 0.000 description 2
- 230000002000 scavenging effect Effects 0.000 description 2
- 239000011669 selenium Substances 0.000 description 2
- 229910052711 selenium Inorganic materials 0.000 description 2
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
- 238000002137 ultrasound extraction Methods 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 1
- 241001037822 Bacillus bacterium Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 240000004160 Capsicum annuum Species 0.000 description 1
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 1
- 235000007862 Capsicum baccatum Nutrition 0.000 description 1
- 244000060011 Cocos nucifera Species 0.000 description 1
- 235000013162 Cocos nucifera Nutrition 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241001573881 Corolla Species 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000001653 FEMA 3120 Substances 0.000 description 1
- 229920002670 Fructan Polymers 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 244000294411 Mirabilis expansa Species 0.000 description 1
- 235000015429 Mirabilis expansa Nutrition 0.000 description 1
- 241000425385 Nepha Species 0.000 description 1
- 241001465805 Nymphalidae Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- 108010020346 Polyglutamic Acid Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 241000120622 Rhizophoraceae Species 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 101710162629 Trypsin inhibitor Proteins 0.000 description 1
- 229940122618 Trypsin inhibitor Drugs 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 235000004552 Yucca aloifolia Nutrition 0.000 description 1
- 235000012044 Yucca brevifolia Nutrition 0.000 description 1
- 244000149006 Yucca filamentosa Species 0.000 description 1
- 235000017049 Yucca glauca Nutrition 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 210000001099 axilla Anatomy 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000001728 capsicum frutescens Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229910052956 cinnabar Inorganic materials 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 229960003067 cystine Drugs 0.000 description 1
- GVJHHUAWPYXKBD-UHFFFAOYSA-N d-alpha-tocopherol Natural products OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 235000013681 dietary sucrose Nutrition 0.000 description 1
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000004626 essential fatty acids Nutrition 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 229920000370 gamma-poly(glutamate) polymer Polymers 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 description 1
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 1
- 235000008696 isoflavones Nutrition 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AIHDCSAXVMAMJH-GFBKWZILSA-N levan Chemical group O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(CO[C@@H]2[C@H]([C@H](O)[C@@](O)(CO)O2)O)O1 AIHDCSAXVMAMJH-GFBKWZILSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 235000013536 miso Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- KNMNNEPMKDJBDW-UHFFFAOYSA-N n-[2-(3,4-dimethoxyphenyl)ethyl]-1-(2,6-dimethylphenoxy)propan-2-amine;hydrochloride Chemical compound Cl.C1=C(OC)C(OC)=CC=C1CCNC(C)COC1=C(C)C=CC=C1C KNMNNEPMKDJBDW-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 235000002949 phytic acid Nutrition 0.000 description 1
- 229940068041 phytic acid Drugs 0.000 description 1
- 239000000467 phytic acid Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- -1 that is Substances 0.000 description 1
- 230000002537 thrombolytic effect Effects 0.000 description 1
- 235000010384 tocopherol Nutrition 0.000 description 1
- 229960001295 tocopherol Drugs 0.000 description 1
- 229930003799 tocopherol Natural products 0.000 description 1
- 239000011732 tocopherol Substances 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 239000011345 viscous material Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A23L11/20—
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/30—Removing undesirable substances, e.g. bitter substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/50—Fermented pulses or legumes; Fermentation of pulses or legumes based on the addition of microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/40—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
- A23L3/44—Freeze-drying
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/10—Drying, dehydrating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/14—Extraction
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/24—Heat, thermal treatment
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/31—Mechanical treatment
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/48—Ultrasonic treatment
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Botany (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
본 발명은 니파야자 꽃대를 이용한 청국장의 제조방법 및 그 청국장에 관한 것으로서, 보다 상세하게는 니파야자 꽃대를 이용하여 청국장을 제조함으로써, 청국장 특유의 불쾌취가 없으며, 관능적 기호도가 우수하고, 기능성이 향상된 청국장을 제조하는 방법에 관한 것이다.The present invention relates to a method for producing chongkukjang by using a nifa palm phalaenopsis, and more particularly, to a method for producing a chonggukjang by using a nifa palm phalaenopsis. More specifically, And more particularly to a method for producing improved chinookguan.
청국장은 콩을 원료로 한 우리나라의 대표적인 발효식품으로서, 간장, 된장, 고추장 등과 함께 오늘날까지 상용되어 온 전통 장류 중의 하나이며, 곡류를 주식으로 하는 우리 민족에게 결핍되기 쉬운 필수아미노산 및 지방산을 공급시켜 주는 식품으로서 중요한 역할을 담당하여 왔다. Cheonggukjang is one of the traditional fermented foods of Korea, which is one of the traditional fermented foods that have been used to date with soy sauce, miso, and kochujang, and it supplies essential amino acids and fatty acids, which are easily deficient to Korean people, The state has played an important role as food.
청국장은 삶은 콩을 볏짚에 붙어 있는 대두를(枯草菌)이라 부르는 바실러스 서브틸리스(Bacillus subtilis)를 이용하여 띄워 만든 것으로서, 발효과정 중에 대두를이 생산하는 효소에 의해서 그 특유의 맛과 냄새를 내는 동시에, 원료콩의 당질과 단백질에서 유래된 레반형프룩탄(levan form fructan)과 폴리글루타메이트(polyglutamate)의 혼합물질인 점질물을 다량 생성하며, 이렇게 발효제조된 청국장은 영양면에서 된장이나 고추장보다 단백질과 지방함량이 높은 양질의 콩 발효식품이다.Chungkookjang is made by using soybean (Bacillus subtilis) called soybean (Bacillus subtilis), which is attached to rice straw, and its unique taste and smell is produced by the enzyme that produces soybean during the fermentation process. At the same time, it produces a large amount of viscous substance, which is a mixture of levan form fructan and polyglutamate, derived from the carbohydrate of the raw soybean and protein. And high-fat soybean fermented food of high quality.
최근 많은 연구자에 의하여 청국장 내에 포함된 활성성분 및 그 역할이 밝혀지고 있는바, 청국장은 콩에서 기인된 이소플라본(isoflavone), 피트산(phytic acid), 사포닌(saponin), 트립신 저해제(trypsin inhibitor), 토코페롤(tocopherol), 불포화지방산, 식이섬유, 올리고당, γ-글루타밀트랜스펩티다제(γ-glutamyltranspeptidase, γ-GTP) 등의 각종 생리활성물질, 항산화 물질 및 혈전용해 효소를 다량 함유하고 있기 때문에, 기능성 식품으로서 그 중요성이 재조명되고 있다.Recently, many researchers have found that the active ingredients contained in Chungkukjang and their roles are known. Chongkukjang contains isoflavone, phytic acid, saponin, trypsin inhibitor, A large amount of various physiologically active substances such as tocopherol, unsaturated fatty acid, dietary fiber, oligosaccharide,? -Glutamyltranspeptidase (? -GTP), antioxidant substances and thrombolytic enzymes , And its importance as a functional food has been reexamined.
그러나 우리나라에서 청국장을 현대화시키는 데에는 뚜렷한 결실이 없었는바, 국민소득이 향상되고 식생활과 주거문화가 서구화되면서, 발효과정과 조리시 야기되는 심한 불쾌취 때문에 청국장의 소비는 제한적이므로, 청국장의 품질을 향상시키고 불쾌취를 억제할 수 있는 제조방법의 확립이 필요한 실정이다.However, since there is no clear result in modernizing Chongkukjang in our country, the consumption of Chungkukjang is limited due to the improvement of national income, westernization of food and residential culture, and severe unpleasantness caused by the process of fermentation and cooking, And it is necessary to establish a manufacturing method capable of suppressing unpleasant odors.
대한민국 공개특허 제10-2002-0021178호에는 기능성 청국장의 제조방법이 개시되어 있고, 대한민국 등록특허 제10-0408964호에는 유카추출물을 첨가하여 풍미를 개선시킨 청국장의 제조방법이 개시되어 있다. Korean Patent Laid-Open No. 10-2002-0021178 discloses a process for producing a functional cheonggukjang, and Korean Patent No. 10-0408964 discloses a process for producing chungkukjang in which the flavor is improved by adding an extract of Yucca.
그러나 선행문헌들의 어디에서도 니파야자의 꽃대를 이용한 청국장은 찾아볼 수 없었다. However, none of the preceding documents found Chinggukjang using the napaya jaeba.
본 발명의 목적은 항산화 활성이 우수한 니파야자의 꽃대를 이용하여 청국장을 제조함으로써, 항산화 활성이 우수하면서도 청국장 특유의 불쾌취는 제거되고, 기호도가 증진된 청국장을 제공하는 것이다.It is an object of the present invention to provide a cheonggukjang which is superior in antioxidative activity while eliminating the unpleasant odor inherent in chonggukjang, and has an increased preference degree by producing chonggukjang using a nipahazana flower having excellent antioxidant activity.
상기 목적을 달성하기 위한 본 발명의 니파야자 꽃대를 이용한 청국장의 제조방법은, (a) 대두를 침지수에 침지시키는 단계와, (b) 상기 침지된 대두를 증자하는 단계와, (c) 상기 증자된 대두에 니파야자 꽃대 분말, 니파야자 꽃대 추출물 또는 니파야자 꽃대의 추출물과 분말 모두를 혼합하는 단계와, (d) 상기 혼합된 원료에 바실러스균을 접종하는 단계와, (e) 상기 바실러스균이 접종된 원료를 발효시키는 단계를 포함하는 것을 특징으로 한다. In accordance with another aspect of the present invention, there is provided a method of producing a chrysanthemum using a nifa palm flower comprising the steps of (a) immersing soybeans in a saliva flask, (b) adding the immersed soybeans, (c) (B) inoculating the mixed raw material with Bacillus sp. G., (C) inoculating the mixed raw material with the Bacillus sp. Flower, And fermenting the inoculated raw material.
상기 니파야자 꽃대 추출물은, 니파야자의 꽃대를 물을 용매로 추출한 것이고, 상기 니파야자 꽃대 분말은, 니파야자 꽃대를 절단하여 물에 데친 후, 탈수, 건조 및 분쇄한 것임을 특징으로 한다. The nifa palm phloem extract is obtained by extracting a flower bud of nipayanas with water as a solvent, and the nifalia phalaenopsis powder is obtained by cutting a nipponia palm flower bed and pouring it in water, followed by dehydration, drying and pulverization.
상기 (c) 단계에서, 상기 (a) 단계의 침지 전 대두 100중량부를 기준으로, 상기 니파야자 꽃대 분말, 니파야자 꽃대 추출물 또는 니파야자 꽃대의 추출물과 분말 모두를 1~20중량부만큼 혼합하는 것을 특징으로 한다.In step (c), 1 to 20 parts by weight of the extracts of the nifolium phalaenopsis powder, the extracts of the nifolium cinnabar or the extracts of the nifa palm are added in an amount of 1 to 20 parts by weight based on 100 parts by weight of the soybean before the dipping .
상기 (c) 단계에서, 홍화씨 분말 1~5중량부를 더 혼합하는 것을 특징으로 한다.In the step (c), 1 to 5 parts by weight of safflower seed powder is further mixed.
상기 (c) 단계에서, 와송의 건조분말 1~5중량부를 더 혼합하는 것을 특징으로 한다.In the step (c), 1 to 5 parts by weight of a dry powder of the feed is further mixed.
상기 (a) 단계의 침지수는 니파야자의 꽃대 추출물을 포함하는 것을 특징으로 한다.The saliva index of the step (a) is characterized in that it contains a flower bud extract of Nipaya.
그리고 그 청국장은 상기한 방법으로 제조되는 것을 특징으로 한다.The cheonggakjang is characterized by being manufactured by the above-mentioned method.
본 발명에 의하면 니파야자 꽃대에 의해 항산화의 기능성을 갖는 청국장의 제조가 가능할 뿐 아니라, 청국장 특유의 불쾌취가 없고, 감칠맛이 우수하여 청국장의 소비를 증진시킬 수 있다는 장점이 있다.According to the present invention, it is possible to manufacture chungkukjang having antioxidative function by the use of the nihon palm phlox, and there is no unpleasant odor peculiar to the chonggukjang, and it is advantageous in that the consumption of chungkukjang can be improved because of the excellent flavor.
또한, 청국장의 보존기한 역시 연장된다는 장점이 있다.In addition, the shelf life of Chungkukjang is also extended.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명의 가장 큰 특징은 상기 청국장의 제조시, 니파야자의 꽃대 추출물, 분말 또는 이들 모두를 첨가하여 제조함으로써, 항산화 활성이 우수하고, 청국장 특유의 불쾌취가 없으며, 관능적 기호도가 우수하다는 것이다. The most remarkable feature of the present invention is that when the chrysanthemum is manufactured by adding the extract of Phalaenopsis nifaya, powder or all of them, the antioxidant activity is excellent, the unpleasant odor unique to chungkukjang does not occur, and the sensory preference is excellent.
여기서, 상기 니파야자(학명 : NIPA FRUTICANS WURMB)는 인도, 말레이시아를 비롯한 동남아시아 및 오스트레일리아가 원산지이며, 맹그로브 지대 등의 습지에서 자란다. 뿌리는 땅속에서 여러 개로 갈라지며, 잎은 지면에서 뭉쳐나고 광택이 있는 녹색을 띤다. 그리고 꽃은 암수한그루이며, 지면의 잎겨드랑이에서 나오는데, 보통 10월에서 이듬해 6월 사이에 꽃대가 올라온다. Here, the above-mentioned NIPPA FRUTICANS WURMB is originated from India, Malaysia, Southeast Asia and Australia, and grows in wetlands such as mangroves. The roots are divided into several pieces in the ground, and the leaves are gathered on the ground and have a glossy green color. And the flower is one male and one female, and it comes out from the axilla of the ground. Usually, the flower stands rise from October to June of the following year.
본 발명은 이러한 니파야자의 꽃대를 채취하고, 이를 가공하여 추출물 또는 분말로서 제조하는 것인데, 특히 니파야자의 꽃대를 사용하는 이유는 니파야자의 다른 어떤 부위보다 꽃대에 폴리페놀 등의 항산화 성분이 풍부하고, 셀레늄 등의 각종 영양성분이 풍부할 뿐 아니라, 우수한 항염증 효능을 보이기 때문이다. 또한, 니파야자의 꽃봉오리에도 인체 유익성분이 다량 함유되어 있으므로, 꽃대에 형성된 꽃봉오리를 제거하지 않고 그대로 사용할 수 있음은 당연하다.The present invention is based on the fact that the flower buds of Nipaya are used for producing extracts or powders, and the reason for using the flower buds of Nipaya is that the flower buds are rich in antioxidant components such as polyphenols It is rich in various nutrients such as selenium, and exhibits excellent anti-inflammatory effects. In addition, since the flower bud of nipayanasia contains a large amount of human-beneficial ingredients, it is natural that the flower bud formed in the flower bud can be used without being removed.
상기 니파야자 꽃대의 성분분석 및 검사 결과를 하기 표 1 및 표 2에 나타내었는바, 상기 니파야자의 꽃대는 폴리페놀, 플라보노이드, 사포닌 등은 물론, 아스파르트산, 티로신, 히스티딘 등의 유효성분을 다량 함유한다. The results of the analysis and the results of the components of the above-mentioned Nihonpalaceae are shown in the following Tables 1 and 2. As shown in Table 1 and Table 2, the Niha-Paya's peduncle contains a large amount of effective components such as polyphenol, flavonoid, saponin, as well as aspartic acid, tyrosine and histidine .
따라서, 이러한 니파야자 꽃대의 추출물 또는 분말을 이용하면, 청국장에 우수한 항산화력이 부여되어 인체에 유익한 효과를 부여하게 되며, 청국장 특유의 불쾌취를 제거하고, 장류의 전체적인 풍미를 개선하는 것은 물론, 보존성을 개선해준다.Therefore, the use of the extract or powder of the nifa palm phlox is imparted with an excellent antioxidant effect on the chongkukjang, thereby imparting a beneficial effect to the human body. In addition, it is possible to eliminate the unpleasant odor inherent in the chonggukjang, It improves preservation.
여기서, 상기 니파야자 꽃대의 추출물은 물을 용매로 하여 추출한 것을 의미한다.Herein, the extract of the above-mentioned Nipah palm phlox is extracted with water as a solvent.
보다 구체적으로는, 니파야자의 꽃대를 3~10cm 정도의 크기로 절단하고, 상기 절단된 니파야자의 꽃대를 물을 용매로 하여 열수추출 또는 초음파 추출한 후, 이를 농축하며, 필요에 따라 건조하여 분말화한다. 여기서, 상기 건조는 동결 건조 또는 분무 건조 등을 이용할 수 있으며, 이는 이 기술이 속하는 분야에서 공지된 방법을 따르므로, 이에 대한 상세한 설명은 생략한다.More specifically, the flower bud of Nipaya is cut to a size of about 3 to 10 cm, and the flower bud of the cut nipaya is subjected to hot water extraction or ultrasonic extraction using water as a solvent, which is then concentrated, I am angry. Here, the drying may be performed by lyophilization, spray drying, or the like, which is well known in the art, and therefore, a detailed description thereof will be omitted.
상기 열수추출이란, 상기 니파야자의 꽃대에 10~20중량배 정도의 물을 가한 후, 80~100℃의 온도에서 10~20시간 추출하는 것이고, 초음파 추출은 니파야자 꽃대에 8~12중량배의 물을 가한 후, 초음파 추출기를 이용하여 100~150kHz 정도의 조건으로 20~30분간 전처리하고, 이를 다시 50~60℃의 온도에서 열수추출을 10~12시간 하는 것이다.The hot water extraction is performed by adding 10 to 20 times by weight of water to the spikelets of the nipayanas and then extracting them at a temperature of 80 to 100 ° C for 10 to 20 hours. The ultrasonic extraction is performed at 8 to 12 times And the mixture is pre-treated for 20 to 30 minutes under the condition of about 100 to 150 kHz by using an ultrasonic wave extractor, and further subjected to hot water extraction for 10 to 12 hours at a temperature of 50 to 60 ° C.
또한, 추출효율을 높이기 위하여 상기 절단된 니파야자를 자연건조, 냉풍건조 또는 열풍건조하여 함수율 10~20%가 되도록 한 후, 이를 100~200mesh 정도로 분쇄하고, 이러한 분말로부터 유효성분을 추출할 수도 있다. 아울러, 상기 절단된 니파야자 꽃대를 100℃의 끓는 물에 10~20분간 데친 후, 상기 데친 꽃대를 탈수하고, 이로부터 유효성분을 추출할 수도 있다.In order to increase the extraction efficiency, the cut nipaya is subjected to natural drying, cold air drying or hot air drying to have a water content of 10 to 20%, followed by pulverizing the dried powder to about 100 to 200 mesh, and extracting the active ingredient from the powder . In addition, it is also possible to dehydrate the cut nippa coconut palms in boiling water at 100 ° C for 10 to 20 minutes, dehydrate them, and extract the active ingredient therefrom.
그리고 상기 농축은 감압 농축하는 것을 의미하는 것으로, 50~60℃에서 용매를 제거하여 20~50Brix 정도로 농축하는 것이다. 또한, 필요에 따라 농축 전, 여과할 수 있음은 당연하다.The concentration means concentration under reduced pressure, and the solvent is removed at 50 to 60 ° C to concentrate the solution to about 20 to 50 Brix. It is of course also possible to conduct filtration before concentration, if necessary.
아울러, 상기 니파야자의 꽃대 분말은, 니파야자 꽃대를 절단하는 단계와, 상기 절단된 니파야자 꽃대를 80~100℃의 물에 20~40분간 데치는 단계와, 상기 데친 니파야자 꽃대를 탈수하는 단계와, 상기 탈수된 니파야자 꽃대를 건조하는 단계와, 상기 건조된 꽃대를 200~1,000mesh로 분쇄하는 단계를 포함한다. In addition, the phalaenopsis powder of Nipaya can be prepared by cutting a nifa palm flower bed, a step of dipping the cut nipa palm flower in water at 80 to 100 ° C for 20 to 40 minutes, a step of dehydrating the wetted nipa palm flower Drying the dehydrated nifa cortex, and pulverizing the dried cortex to 200 to 1,000 meshes.
즉, 먼저 니파야자의 꽃대를 채취하고 깨끗이 세척한 후, 이를 3~10cm의 길이로 절단한다. 다음으로, 상기 절단된 꽃대를 고온의 물, 즉 80~100℃의 물에 20~40분간 데쳐내고, 상기 데친 꽃대를 찬물에 담가 식힌 후, 길이방향, 즉, 결대로 길게 찢는다. 이때, 그 두께는 대략 0.5~1cm 정도가 바람직하다. 상기 니파야자의 꽃대는 길이방향으로 나란한 결을 가지기 때문에 쉽게 결대로 쉽게 찢어진다. 다음으로, 상기 찢은 꽃대에 탈수하고, 열풍건조, 냉풍건조 또는 일광에 의한 자연건조법을 통해 함수율이 5~10%가 되도록 건조한 후, 상기 건조된 꽃대를 200~1,000mesh로 분쇄기 또는 제분기를 이용하여 분쇄함으로써, 그 제조를 완료한다. 본 발명에서 상기 탈수방법은 제한하지 않는 것으로, 별도의 탈수장치를 이용하거나, 손으로 눌러 탈수할 수 있다.In other words, first take a flower bud of Nipaya, clean it, and cut it to a length of 3 ~ 10cm. Next, the cut flower stalks are heated in hot water, that is, water at 80 to 100 ° C for 20 to 40 minutes, and the dulled flower buds are soaked in cold water and then ripped in the longitudinal direction, At this time, the thickness is preferably about 0.5 to 1 cm. The nipayanaya peduncle is easily torn as easily because it has parallel longitudinal texture. Next, the tears are dewatered on the torn flower buds, dried by hot air drying, cold air drying or natural drying by daylight to a moisture content of 5 to 10%, and then the dried flower buds are pulverized or milled Followed by pulverization, thereby completing the production. In the present invention, the dewatering method is not limited, and a separate dewatering device may be used or dewatered by hand.
이하, 본 발명을 상세히 설명한다. 이하에서의 니파야자 꽃대 추출물과 니파야자 꽃대 분말은 상기에서 설명한 바와 같이 제조된 것을 의미한다.Hereinafter, the present invention will be described in detail. Hereinafter, the term " nifa palm phloem extract " and " nifa palm phalaenopsis powder " means those prepared as described above.
먼저, 본 발명에 따른 청국장의 제조방법은, 보다 구체적으로 (a) 대두를 침지수에 침지시키는 단계와, (b) 상기 침지된 대두를 증자하는 단계와, (c) 상기 증자된 대두에 니파야자 꽃대 분말, 니파야자 꽃대 추출물 또는 니파야자 꽃대의 추출물과 분말 모두를 혼합하는 단계와, (d) 상기 혼합된 원료에 바실러스균을 접종하는 단계와, (e) 상기 바실러스균이 접종된 원료를 발효시키는 단계를 포함하는 것을 특징으로 한다. The method of manufacturing a cheonggukjang according to the present invention comprises the steps of: (a) immersing soybeans in a saliva flour; (b) growing the immersed soybeans; (c) (D) inoculating the mixed raw material with Bacillus spp., And (e) injecting the raw material in which the Bacillus spp. Is inoculated into the raw material of the Bacillus spp. And a step of fermentation.
이하, '중량부'는 상기 (a) 단계에서 사용되는 대두, 즉 침지수에 침지시키기 전의 대두 100중량부를 기준으로 한다. Hereinafter, the 'weight part' is based on 100 parts by weight of soybeans used in the step (a), that is, soybeans before soaked in the soaking index.
(a) 대두를 침지수에 침지시키는 단계.(a) immersing the soybeans in a saliva flask.
먼저, 대두를 깨끗이 세척하여 대두에 부착된 이물질을 제거하고, 10 내지 30℃의 침지수를 대두 부피의 2~5배 정도의 양으로 투입한 후, 10~15시간 동안 침지시킨다. First, the soybeans are cleanly washed to remove foreign matter adhering to the soybeans, and the saliva index at 10 to 30 ° C is added in an amount of about 2 to 5 times the volume of the soybeans, followed by immersion for 10 to 15 hours.
이때, 상기 대두의 침지시간이 10시간 미만이면 대두가 충분히 불지 않아 후공정인 증자 및 발효가 원활하지 않으며, 15시간을 초과하면 비효율적이기 때문이다. If the immersion time of the soybeans is less than 10 hours, the soybeans are not sufficiently blown, so that the subsequent fermentation and fermentation are not smooth, and if the immersion time is longer than 15 hours, the soybeans are inefficient.
여기서, 상기 침지수로는 물을 사용할 수 있다. 아울러, 항산화 효능 및 기호도의 증가를 위하여 니파야자 꽃대 추출물을 침지수로서 사용할 수도 있다. 여기서, 상기 니파야자의 꽃대 추출물은 추출 후 여과만을 실시하고, 농축 및 건조하지 않은 액상의 상태의 것을 사용할 수 있으나, 건조한 분말 상의 추출물을 물과 혼합하여 사용하는 것도 가능하다. The immersion water may be water. In addition, for the purpose of increasing the antioxidant efficacy and the degree of preference, the extract of Nihon palaceae may be used as a needle index. Here, the flower bud extract of Niepaya can be used in the form of a liquid which is extracted and filtered only and is not concentrated or dried, but it is also possible to use a dry powdery extract in admixture with water.
(b) 상기 침지된 대두를 증자하는 단계.(b) growing the immersed soybeans.
다음으로, 상기 침지된 대두를 증자한다. Next, the immersed soybean is added.
상기 증자는 90~100℃로 1~10시간 동안 증자한 후, 잔열을 이용하여 10분~2시간 동안 뜸을 들이는 것으로, 이러한 증자단계는 공지된 방법에 따른다. The enhancement is carried out at 90 to 100 ° C for 1 to 10 hours, and thereafter heated for 10 minutes to 2 hours using the residual heat. The growth step is performed according to a known method.
(c) 상기 증자된 대두에 (c) 니파야자Nipa 꽃대 분말, Phalaenopsis powder, 니파야자Nipa 꽃대 추출물 또는 Flower bud extract or 니파야자Nipa 꽃대의 추출물과 분말 모두를 혼합하는 단계. The step of mixing both the extract of the flower bud and the powder.
그리고 상기 증자된 대두에 니파야자 꽃대 분말, 니파야자 꽃대 추출물 또는 니파야자 꽃대의 추출물과 분말 모두를 혼합한다.Then, the above-mentioned soybean is mixed with both the extract of Nipahaya phalaenopsis powder, the extract of Nipha palm phloem or the extract of Nipha palm phalaenopsis and the powder.
상기 니파야자 꽃대 분말과 니파야자 꽃대 추출물은 청국장의 불쾌취를 제거해주며, 항산화력을 부여하고, 감칠맛을 향상시켜 풍미를 좋게 하며, 보존성 역시 개선해주는 역할을 한다. 이때, 그 혼합비는 상기 (a) 단계의 대두 100중량부에 대하여 니파야자 꽃대 분말, 니파야자 꽃대 추출물 또는 니파야자 꽃대의 추출물과 분말 모두를 1~20중량부만큼 혼합함이 바람직한바, 그 혼합비가 1중량부 미만이면 효과가 미미하고, 20중량부를 초과하면 오히려 청국장의 구수한 풍미가 저하되기 때문이다. The above-mentioned Nihon palm phalaenopsis powder and Nepha palm phlox extract remove the unpleasant odor of Chongkukjang, impart antioxidant power, improve the flavor and flavor, and improve the preservation. At this time, it is preferable to mix 1 to 20 parts by weight of the extracts and the powders of the Nipahaya phalaenopsis powder, the extract of the Nipha palm fruit, or the extract of the Nipha palm flower with respect to 100 parts by weight of the soybean in the step (a) If the amount is less than 1 part by weight, the effect is insignificant. If the amount is more than 20 parts by weight, the fragrant flavor of the cheonggukjang is lowered.
(d) 상기 혼합된 원료에 (d) adding to the mixed raw material 바실러스균을Bacillus bacteria 접종하는 단계. Inoculation step.
다음으로, 상기 혼합된 원료에 바실러스균(Bacillus subtilis)을 접종한다. Next, the mixed raw material is inoculated with Bacillus subtilis.
여기서, 상기 바실러스균의 종류 및 접종량은 종래 공지된 것에 따르며, 예시적으로 상기 (a) 단계에서 사용된 침지 전 대두 100중량부를 기준으로, 0.5~2중량부만큼 접종한다. Herein, the kind and the inoculum amount of the Bacillus fungi are conventionally known, and are exemplarily inoculated in an amount of 0.5 to 2 parts by weight based on 100 parts by weight of the pre-soaked soybean used in the step (a).
(e) 상기 (e) 바실러스균이Bacillus 접종된 원료를 발효시키는 단계 Step of fermenting the inoculated raw material
그리고 상기 바실러스균이 접종된 원료를 38~50℃의 온도 및 80~90%의 습도에서 40~72시간 발효시킨다.The raw material inoculated with the Bacillus subtilis is fermented at a temperature of 38 to 50 DEG C and a humidity of 80 to 90% for 40 to 72 hours.
이때, 상기 온도 범위를 벗어나는 경우 바실러스균의 활성이 저하되며 증식이 원활히 되지 않아 대두의 발효가 제대로 일어나지 않고, 상기 발효시간이 40시간 미만이면 발효가 충분히 진행되지 않아 청국장의 풍미가 덜하여지며, 72시간을 초과하면 과발효로 인해 대두가 오히려 굳어지며 청국장의 퀴퀴한 냄새가 심해지기 때문이다.At this time, if the temperature is out of the above range, the activity of Bacillus bacterium is lowered and the fermentation of soybeans does not occur properly due to insufficient growth. If the fermentation time is less than 40 hours, the fermentation is not sufficiently progressed, If it exceeds 72 hours, the soybean becomes hardened due to over fermentation and the smell of Chungkukjang becomes worse.
한편, 상기 (c) 단계에서, 상기 (a) 단계에서 사용된 침지 전 대두 100중량부를 기준으로, 홍화씨 분말 1~5중량부를 더 혼합할 수도 있는바, 상기 홍화씨 분말을 더 포함하면 청국장의 불쾌취는 효과적으로 제거되고, 보존성은 더욱 개선될 수 있기 때문이다.Meanwhile, in step (c), 1 to 5 parts by weight of safflower seed powder may be further mixed with 100 parts by weight of soybeans before soaking used in step (a). If the safflower seed powder is further included, The pores are effectively removed and the preservability can be further improved.
또한, 상기 (c) 단계에서, 상기 (a) 단계에서 사용된 침지 전 대두 100중량부를 기준으로, 와송 분말 1~5중량부를 더 혼합할 수도 있는바, 와송의 건조분말을 더 포함하면, 항산화력이 더욱 우수할 뿐 아니라, 청국장의 감칠맛이 증가하는 효과가 있기 때문이다.Also, in step (c), 1 to 5 parts by weight of the feed powder may be further mixed with 100 parts by weight of soybeans before immersion, which is used in step (a) Not only is it better in strength, it also has an effect of increasing the richness of Chungkookjang.
여기서, 상기 와송과 홍화씨의 분말은, 홍화씨를 열풍 또는 동결 건조한 것이거나, 와송을 동결건조하여 제조할 수 있는바, 이러한 와송과 홍화씨 분말은 시판 상품을 사용할 수 있다. Herein, the powder of the safflower and safflower seed can be produced by hot-air drying or freeze-drying the safflower seed, or by freeze-drying the safflower, and the safflower seed and the safflower seed powder can be commercial products.
또한, 상기와 같이 발효된 청국장에 천일염, 고춧가루 등을 추가로 혼합할 수 있는 것으로, 종래 청국장이 속하는 분야에서 공지된 첨가제의 혼합은 제한하지 않는다.In addition, the above-described fermented chonggukjang can further be mixed with sun-dried salt, red pepper powder, etc. The mixing of known additives in the field of the conventional chonggukjang is not limited.
상기와 같이 제조된 본 발명의 청국장은 불쾌취가 제거되고, 감칠맛이 우수하며, 항산화활성이 우수하다는 장점이 있다. 아울러, 보존성 역시 우수하다. The cheonggukjang prepared according to the present invention has the advantage of being free from unpleasant odor, having excellent flavor and excellent antioxidant activity. In addition, preservation is also excellent.
이하, 본 발명을 하기의 실시예를 통해 더욱 상세히 설명한다.Hereinafter, the present invention will be described in more detail with reference to the following examples.
(실시예 1:니파야자 꽃대 추출물의 제조)(Example 1: Preparation of Extract of Isolate Nymphalidae)
니파야자 꽃대를 채취하고, 깨끗이 세척한 후, 이를 10cm 길이로 절단하였다. 50℃의 열풍으로 건조하여 함수율이 15%가 되도록 하고, 이를 100mesh 정도로 분쇄한 후, 이에 20중량배의 증류수를 가하고, 90~100℃에서 20시간 동안 추출하였다. 그리고 이를 여과한 후, 감압농축기를 이용하여 50℃에서 감압 농축하고, 동결건조하여 분말 상의 추출물을 얻었다.The nipponia palms were picked, cleaned and then cut into 10 cm lengths. Dried by hot air at 50 캜 to have a water content of 15%, and pulverized to about 100 mesh. 20 wt. Parts of distilled water was added thereto, and the mixture was extracted at 90 to 100 캜 for 20 hours. After filtration, the filtrate was concentrated under reduced pressure at 50 ° C using a vacuum concentrator and lyophilized to obtain a powdery extract.
(실시예 2: 니파야자 꽃대 분말의 제조)(Example 2: preparation of nipah palaceae powder)
니파야자 꽃대를 채취하고, 깨끗이 세척한 후, 이를 10cm 길이로 절단하였다. 그리고 이를 100℃의 끓는 물에 30분간 데치고, 데친 꽃대를 찬물에 담가 식혔다. 다음으로, 상기 데친 꽃대를 직경 0.8cm 정도로 결대로 찢어준 후, 탈수기에 넣어 탈수하였다. 그리고 이를 60℃의 열풍건조기를 이용하여 수분함량이 7%가 되도록 건조하였다. 다음으로, 건조된 꽃대를 분쇄기로 1차 및 2차 분쇄하여 약 300mesh의 입도를 갖는 분말을 제조하였다. The nipponia palms were picked, cleaned and then cut into 10 cm lengths. Then, it was boiled in boiling water at 100 ° C for 30 minutes, and the vestibule was soaked in cold water and cooled. Next, the corolla was torn to a diameter of about 0.8 cm, and dehydrated in a dehydrator. Then, it was dried at a moisture content of 7% by using a hot-air dryer at 60 ° C. Next, the dried peduncle was first and second pulverized with a pulverizer to prepare a powder having a particle size of about 300 mesh.
(실시예 3: 니파야자 꽃대 추출물을 이용한 청국장의 제조)(Example 3: Preparation of Chungkukjang using the extract of Nihon palaceae)
대두 10kg를 세척한 후, 이에 20℃의 물 30kg을 투입한 후, 10시간 동안 침지시켰다. 그리고 상기 침지된 대두를 90~100℃로 8시간 동안 증자한 후, 잔열로 1시간 동안 뜸을 들였다. 다음으로, 상기 증자된 대두에 실시예 1의 니파야자 꽃대 추출물 500g을 투입하여 혼합하고, 이에 바실러스균 150g을 접종하였다. 그리고 이를 39℃의 온도 및 80%의 습도에서 48시간 발효시켰다.After washing 10 kg of soybeans, 30 kg of water at 20 캜 was added thereto, and then immersed for 10 hours. Then, the immersed soybeans were heated at 90 to 100 ° C for 8 hours, and then steamed for one hour with residual heat. Next, 500 g of the extract of Example 1 was added to the thus-added soybean, and 150 g of Bacillus bacteria was inoculated thereto. And it was fermented at a temperature of 39 DEG C and a humidity of 80% for 48 hours.
이때, 상기 바실러스균은 대두 분말 10g, 영양배지(Beef extract 3g, peptone 5g) 8g을 증류수 150㎖에 녹여 300㎖ 플라스크에 넣어 자동멸균기(1.5 ㎏/㎠ 121℃ 15분간)에서 살균한 후 바실러스균(Bacillus subtilis KCCM 12027)을 접종한 다음 40℃의 배양기에서 48시간 배양한 것(107~1010CFU/ml)을 사용하였다. 8 g of soybean powder, 10 g of soybean powder, 3 g of beef extract, 5 g of peptone were dissolved in 150 ml of distilled water and sterilized in a 300 ml flask using an automatic sterilizer (1.5 kg / cm 2 at 121 ° C for 15 minutes) (Bacillus subtilis KCCM 12027) and cultured in a 40 ° C incubator for 48 hours (10 7 to 10 10 CFU / ml) was used.
(실시예 4: 니파야자 꽃대 분말을 이용한 청국장의 제조)(Example 4: Preparation of Chungkukjang using a nifa palm phalaenopsis powder)
실시예 3과 동일하게 실시하되, 실시예 1의 추출물을 대신하여 실시예 2의 분말을 사용하였다. The procedure of Example 3 was repeated except that the powder of Example 2 was used in place of the extract of Example 1.
(실시예 5: 니파야자 꽃대 추출물과 니파야자 꽃대 분말을 이용한 청국장의 제조)(Example 5: Preparation of Chungkukjang using the extracts of Nihonpalaceae and Nihonpalaceae)
실시예 3과 동일하게 실시하되, 실시예 1의 추출물을 대신하여 실시예 1의 추출물과 실시예 2의 분말을 1:1 중량비로 혼합하여 사용하였다. Except that the extract of Example 1 and the powder of Example 2 were mixed at a weight ratio of 1: 1 in place of the extract of Example 1.
(실시예 6: 와송 분말을 추가하여 청국장을 제조)(Example 6: Chongkukjang was prepared by adding a waxy powder)
실시예 3과 동일하게 실시하되, 시판되는 동결건조 와송 분말을 구입하고, 상기 증자된 대두에 와송분말 200g을 상기 실시예 1의 추출물과 함께 혼합하였다. A commercially available lyophilized dried powder was obtained in the same manner as in Example 3, and 200 g of the dried powder was mixed with the extract of Example 1.
(비교예 1)(Comparative Example 1)
실시예 3과 동일하게 실시하되, 니파야자 꽃대 추출물은 사용하지 않았다.The same procedure as in Example 3 was carried out except that the extract of Nephala palm is not used.
(시험예 1)(Test Example 1)
니파야자 꽃대 추출물의 항산화력 평가.Evaluation of Antioxidative Capacity of Extracts of.
실시예 1과 같이 제조한 니파야자 꽃대 추출물의 항산화력을 측정하였다.The antioxidative activity of the extracts of Nephala palmetae prepared in Example 1 was measured.
상기 추출물은 냉동보관하면서 실험에 사용하였다.The extract was used in the experiment while being kept frozen.
(1) DPPH radical 소거능 측정(1) Measurement of DPPH radical scavenging ability
항산화 활성 측정은 DDPH(1,1-diphenyl-2-picryhydrazyl, Sigma-Aldrich Co.)를 이용하여 시료의 라디칼 소거효과를 보는 Biois법을 활용하였다. 각 추출물을 농도별 0.025~10mg/ml로 제조한 시료 용액 1ml에 0.2mM DPPH 1ml를 넣고, vortex mixer를 사용하여 혼합하고 암실에서 30분간 반응시킨 후 517nm에서 흡광도를 측정하였다. 그리고 아래의 식을 이용하여 free radical scavenging activity 곡선을 작성한 후, IC50을 산출하였으며, 양성대조군으로 ascorbic acid를 사용하였다. To determine the antioxidant activity, the Biois method was used to examine the radical scavenging effect of DDPH (1,1-diphenyl-2-picryhydrazyl, Sigma-Aldrich Co.). 1 ml of 0.2 mM DPPH was added to 1 ml of the sample solution prepared from 0.025 to 10 mg / ml of each extract, mixed using a vortex mixer, reacted for 30 minutes in a dark room, and absorbance was measured at 517 nm. Then, free radical scavenging activity curves were generated using the following equation, and IC 50 was calculated. As a positive control, ascorbic acid was used.
Radical scavenging activity(%)={(control Absorbance - Sample Absorbance)/control Absorbance}×100Radical scavenging activity (%) = {(control Absorbance - Sample Absorbance) / control Absorbance} × 100
(2) ABTS radical 소거능 측정(2) Measurement of ABTS radical scavenging ability
ABTS radical 소거능 측정은 Pellegrin 등의 방법으로 측정하였다. 7mM ABTS(2,2'-azinobis(3-ethylenbenzothiazoline-6-sulfonate), Sigma-Aldrich Co.) 5ml와 140mM K2S2O8(Sigma-Aldrich Co.) 88㎕를 잘 섞어 16시간 이상 암소에 방치시켰다. ABTS radical scavenging activity was measured by Pellegrin et al. 5 ml of 7 mM ABTS (2,2'-azinobis (3-ethylenbenzothiazoline-6-sulfonate), Sigma-Aldrich Co.) and 88 μl of 140 mM K 2 S 2 O 8 (Sigma-Aldrich Co.) .
이를 absolute ethanol과 1:88 비율로 섞어 734nm에서 대조구의 흡광도 값이 0.7±0.002가 되도록 조절한 ABTS solution을 만들었다. 시료 용액 50㎕에 ABTS solution 1ml를 넣어 혼합하고, 2.5분간 암실에서 방치한 후, 734nm에서 흡광도를 측정하였다.The mixture was mixed with absolute ethanol at a ratio of 1:88, and the ABTS solution was prepared so that the absorbance of the control was adjusted to 0.7 ± 0.002 at 734 nm. 1 ml of ABTS solution was added to 50 μl of the sample solution, and the mixture was allowed to stand in a dark room for 2.5 minutes, and the absorbance was measured at 734 nm.
그 결과는 하기 표 3 및 표 4에 나타내었다.The results are shown in Tables 3 and 4 below.
(mg/ml)Concentration
(mg / ml)
(mg.ml)IC 50
(mg.ml)
0.125
0.0625
0.03125
0.156250.025
0.125
0.0625
0.03125
0.15625
51.52±1.09
26.74±1.51
13.93±2.09
7.82±0.3785.83 + - 0.22
51.52 ± 1.09
26.74 1.51
13.93 ± 2.09
7.82 + - 0.37
0.5
0.25
0.125
0.0625One
0.5
0.25
0.125
0.0625
44.73±0.74
36.62±0.49
30.87±0.41
28.79±0.4160.70 + - 0.47
44.73 + - 0.74
36.62 ± 0.49
30.87 + - 0.41
28.79 + - 0.41
0.5
0.25
0.125
0.0625One
0.5
0.25
0.125
0.0625
37.41±0.41
32.67±0.43
30.27±0.14
28.98±0.4344.89 + - 0.41
37.41 + - 0.41
32.67 + - 0.43
30.27 + - 0.14
28.98 + - 0.43
IC50 값은 추출물을 첨가하지 않은 대조군의 값을 50% 감소시키는 추출물의 농도를 나타낸 것으로, IC50 값이 작을수록 항산화 활성이 높은 것을 뜻한다. 따라서 상기 표 3에서와 같이, DPPH radical scavenging activity 측정 결과에 따르면 니파야자 꽃대의 항산화능은 블루베리에 비하여 약 40배 높고, 홍삼에 비해 약 75배 높음을 알 수 있었다.The IC 50 value represents the concentration of the extract which reduces the value of the control without the extract by 50%. The smaller the IC 50 value, the higher the antioxidant activity. Therefore, as shown in Table 3, according to DPPH radical scavenging activity measurement results, the antioxidant activity of the Nypha palm phlox was about 40 times higher than that of blueberry and about 75 times higher than that of red ginseng.
(mg/ml)Concentration
(mg / ml)
(mg.ml)IC 50
(mg.ml)
0.125
0.0625
0.03125
0.156250.025
0.125
0.0625
0.03125
0.15625
23.28±0.99
11.54±0.94
5.87±0.27
2.50±0.5644.44 + - 0.42
23.28 ± 0.99
11.54 + - 0.94
5.87 ± 0.27
2.50 ± 0.56
0.5
0.25
0.125
0.0625One
0.5
0.25
0.125
0.0625
10.05±0.48
5.40±0.17
3.12±0.15
2.08±0.5917.68 ± 0.51
10.05 + 0.48
5.40 ± 0.17
3.12 ± 0.15
2.08 ± 0.59
0.5
0.25
0.125
0.0625One
0.5
0.25
0.125
0.0625
6.09±0.51
3.02±0.31
1.58±0.56
0.25±0.3111.89 + - 0.54
6.09 ± 0.51
3.02 ± 0.31
1.58 ± 0.56
0.25 0.31
상기 표 4에서와 같이, ABTS radical scavenging activity 측정 결과에 따르면, 니파야자 꽃대의 항산화능은 블루베리에 비하여 약 42배 높고, 홍삼에 비해 약 59배 높음을 알 수 있었다.As shown in Table 4, according to the result of ABTS radical scavenging activity measurement, the antioxidant activity of Nypha palm phlox was about 42 times higher than that of blueberry and about 59 times higher than that of red ginseng.
(시험예 2)(Test Example 2)
이취 측정Odor measurement
시중에 구입한 청국장을 대조군으로 하여 상기 실시예들 및 비교예 1에서 제조된 청국장의 이취 정도를 측정하여 하기 표 5에 나타내었다. 이취의 측정은 각 청국장을 취하여 각각에 대해 삼각 플라스크에 넣고 밀폐시킨 후, 1분간 진탕하고 5분간 방치한 다음 가스텍 시료흡입기를 이용하여 암모니아 함량을 측정하였다.The degree of the odor of the chungkukjang prepared in the above Examples and Comparative Example 1 was measured using a commercially available cheonggukjang as a control, and the results are shown in Table 5 below. For the measurement of odor, each Cheonggukjang was put into a Erlenmeyer flask, and each sample was shaken for 1 minute and allowed to stand for 5 minutes. Then, the ammonia content was measured using a Gastec sample inhaler.
상기 표 5에서와 같이 본 발명에 따른 실시예들이 비교예 1 및 대조군에 비하여 암모니아 성분이 현저히 낮음을 알 수 있었다. As shown in Table 5, it can be seen that the examples according to the present invention have significantly lower ammonia components than those of Comparative Example 1 and the control group.
(시험예 3)(Test Example 3)
저장성 측정Storage stability measurement
상기 실시예들 및 비교예 1에서 제조된 청국장을 공기와 접촉된 상태에서 고온다습(35℃, 80%)한 장소에 정치한 다음, 시각 및 후각을 이용하여 부패 여부를 관찰하고 부패 발생이 처음 관찰된 날을 하기 표 6에 나타내었다.The chrysanthemum plants prepared in the above Examples and Comparative Example 1 were placed in a hot and humid (35 ° C, 80%) place in contact with air and then observed for corruption using time and smell, The observed date is shown in Table 6 below.
상기 표 6에서와 같이 본 발명에 따른 실시예들이 비교예 1에 비하여 늦게 부패가 발생하였음을 확인하였다. 아울러, 실시예 6이 가장 부패가 늦게 발생함을 확인하였다.As shown in Table 6, it was confirmed that the examples according to the present invention had a late corruption as compared with Comparative Example 1. In addition, it was confirmed that the corruption occurred most slowly in Example 6.
(시험예 4)(Test Example 4)
관능검사Sensory test
상기 실시예들 및 비교예 1에서 제조된 청국장은, 청국장은 관능검사를 위해 물 200mL에 상기 청국장 50g을 넣은 후 5분간 끓여 제공하였다.The cheonggukjang prepared in the above Examples and Comparative Example 1 was prepared by adding 50 g of the above-mentioned chungkukjang to 200 ml of water for a sensory test and boiling for 5 minutes.
상기 관능 검사는 잘 훈련받은 관능요원 20명을 대상으로 향, 색, 맛 및 전체적인 기호도를 평가하게 한 후, 평균값을 하기 표 7에 나타내었다.The sensory test was carried out by 20 well-trained sensory agents to evaluate flavor, color, taste and overall acceptability, and the average values are shown in Table 7 below.
- 5점: 매우 우수하다- 5 points: Very good
- 4점: 우수하다- 4 points: Excellent
- 3점: 보통- 3 points: Normal
- 2점: 좋지 않다- 2 points: Not good
- 1점: 매우 좋지 않다- 1 point: Not very good
상기 표 6에서 살펴본 바와 같이, 니파야자의 꽃대 분말 또는 추출물을 포함하는 실시예 3 내지 6의 청국장은 비교예 1에 비하여 향, 맛 및 전체적인 기호도가 우수함을 확인하였다. As shown in Table 6, it was confirmed that the cheonggukjang of Examples 3 to 6 containing the phalaenopsis powder or extract of nipaya was superior in flavor, taste and overall acceptability to Comparative Example 1.
(실시예 7~11)(Examples 7 to 11)
실시예 3과 동일하게 실시하되, 니파야자 꽃대 추출물을 각각 50g, 100g, 1kg, 2kg, 3kg 혼합하였다. The same procedure as in Example 3 was carried out except that the extracts of Nephala palm are 50 g, 100 g, 1 kg, 2 kg and 3 kg, respectively.
(시험예 8)(Test Example 8)
관능검사Sensory test
상기 시험예 7과 동일한 방법으로, 실시예 7 내지 11의 관능검사를 실시하였다. 그리고 그 결과는 하기 표 7에 나타내었다.The sensory tests of Examples 7 to 11 were carried out in the same manner as in Test Example 7. The results are shown in Table 7 below.
상기 표 7에서와 같이 실시예 7 내지 11은 모두 그 기호도가 우수함을 확인하였다. 그러나 실시예 7과 실시예 11은 그 기호도가 실시예 8, 9, 10에 비하여 다소 높지 못하였다.As shown in Table 7, all of Examples 7 to 11 confirmed that the degree of preference was excellent. However, in Examples 7 and 11, the degree of preference was not higher than that in Examples 8, 9 and 10. [
이상, 본 발명을 바람직한 실시예를 사용하여 상세히 설명하였으나, 본 발명의 범위는 특정 실시예에 한정되는 것은 아니며, 첨부된 특허청구범위에 의하여 해석되어야 할 것이다. 또한, 이 기술분야에서 통상의 지식을 습득한 자라면, 본 발명의 범위에서 벗어나지 않으면서도 많은 수정과 변형이 가능함을 이해하여야 할 것이다.While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the scope of the present invention is not limited to the disclosed exemplary embodiments. It will also be appreciated that many modifications and variations will be apparent to those skilled in the art without departing from the scope of the present invention.
Claims (7)
(b) 상기 침지된 대두를 증자하는 단계와,
(c) 상기 증자된 대두에 니파야자 꽃대 분말, 니파야자 꽃대 추출물 또는 니파야자 꽃대의 추출물과 분말 모두를 혼합하는 단계와,
(d) 상기 혼합된 원료에 바실러스균을 접종하는 단계와,
(e) 상기 바실러스균이 접종된 원료를 발효시키는 단계를 포함하는 것을 특징으로 하는 니파야자 꽃대를 이용한 청국장의 제조방법.
(a) immersing the soybeans in a saliva flask,
(b) increasing the soaked soybeans;
(c) mixing the extracted soybean with both the extract of the nifolium phalaenopsis powder, the extract of the nifolium phalaenopsis or the extract of the nifa palm flower, and the powder,
(d) inoculating the mixed raw material with Bacillus sp.
(e) fermenting the raw material inoculated with the Bacillus subtilis.
상기 니파야자 꽃대 추출물은,
니파야자의 꽃대를 물을 용매로 추출한 것이고,
상기 니파야자 꽃대 분말은,
니파야자 꽃대를 절단하여 물에 데친 후, 탈수, 건조 및 분쇄한 것임을 특징으로 하는 니파야자 꽃대를 이용한 청국장의 제조방법.
The method according to claim 1,
The above-mentioned < RTI ID = 0.0 >
It is extracted with water as a spear of nipaya,
The above-mentioned < RTI ID = 0.0 >
A method for producing chungkukjang using a nihon palm phalanx characterized in that the nihon palaceae is cut and poured into water, followed by dewatering, drying and grinding.
상기 (c) 단계에서,
상기 (a) 단계의 침지 전 대두 100중량부를 기준으로,
상기 니파야자 꽃대 분말, 니파야자 꽃대 추출물 또는 니파야자 꽃대의 추출물과 분말 모두를 1~20중량부만큼 혼합하는 것을 특징으로 하는 니파야자 꽃대를 이용한 청국장의 제조방법.
The method according to claim 1,
In the step (c)
Based on 100 parts by weight of soybeans before immersion in step (a)
Wherein the extract of Nephala palm phlox, the extract of Nephala palm or the extract and powder of Nephala palm are mixed in an amount of 1 to 20 parts by weight.
상기 (c) 단계에서,
홍화씨 분말 1~5중량부를 더 혼합하는 것을 특징으로 하는 니파야자 꽃대를 이용한 청국장의 제조방법.
The method of claim 3,
In the step (c)
And 1 to 5 parts by weight of safflower seed powder is further mixed.
상기 (c) 단계에서,
와송의 건조분말 1~5중량부를 더 혼합하는 것을 특징으로 하는 니파야자 꽃대를 이용한 청국장의 제조방법.
The method of claim 3,
In the step (c)
And 1 to 5 parts by weight of a dried powder of the soybean curd is further mixed.
상기 (a) 단계의 침지수는 니파야자의 꽃대 추출물을 포함하는 것을 특징으로 하는 니파야자 꽃대를 이용한 청국장의 제조방법.
The method according to claim 1,
Wherein the saliva index of the step (a) comprises a flower bud extract of nipaya.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020160152312A KR101870419B1 (en) | 2016-11-16 | 2016-11-16 | Method for preparing chungkookchang comprising nipa fruticans wurmb and chungkookchang by the method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020160152312A KR101870419B1 (en) | 2016-11-16 | 2016-11-16 | Method for preparing chungkookchang comprising nipa fruticans wurmb and chungkookchang by the method |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20180055033A true KR20180055033A (en) | 2018-05-25 |
KR101870419B1 KR101870419B1 (en) | 2018-06-27 |
Family
ID=62299666
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020160152312A KR101870419B1 (en) | 2016-11-16 | 2016-11-16 | Method for preparing chungkookchang comprising nipa fruticans wurmb and chungkookchang by the method |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR101870419B1 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102429269B1 (en) | 2021-12-20 | 2022-08-05 | 주식회사 양수마루 | Manufacturing method of cheonggukjang stew and cheonggukjang stew manufactured by this manufacturing method |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20020021178A (en) | 2000-08-23 | 2002-03-20 | 박건영 | functional Chungkookjang and process for preparation thereof |
KR20020078314A (en) * | 2001-04-09 | 2002-10-18 | 조갑환 | Manufacturing method of health foods using an animal and a plant |
KR100408964B1 (en) | 2001-03-13 | 2003-12-06 | 주식회사 쎌텍스 | Manufacturing method for improving flavor of chungkookjang by adding yucca extracts |
KR101282587B1 (en) * | 2011-04-08 | 2013-07-04 | 신덕자 | Method for Preparing Chungkukjang Using Bamboo Shoot |
-
2016
- 2016-11-16 KR KR1020160152312A patent/KR101870419B1/en active IP Right Grant
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20020021178A (en) | 2000-08-23 | 2002-03-20 | 박건영 | functional Chungkookjang and process for preparation thereof |
KR100408964B1 (en) | 2001-03-13 | 2003-12-06 | 주식회사 쎌텍스 | Manufacturing method for improving flavor of chungkookjang by adding yucca extracts |
KR20020078314A (en) * | 2001-04-09 | 2002-10-18 | 조갑환 | Manufacturing method of health foods using an animal and a plant |
KR101282587B1 (en) * | 2011-04-08 | 2013-07-04 | 신덕자 | Method for Preparing Chungkukjang Using Bamboo Shoot |
Non-Patent Citations (1)
Title |
---|
Lim T.K. Edible Medicinal and Non-Medicinal Plants. Vol. 1, Fruits. Chapter 50, Nypa Fruticans, pp402-406 (2011.09.29.)* * |
Also Published As
Publication number | Publication date |
---|---|
KR101870419B1 (en) | 2018-06-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR102081844B1 (en) | Manufacturing method for red ginseng slice chocolate and red ginseng slice chocolate manufactured by the same | |
KR101682121B1 (en) | Manufacturing method for Kochujang-gulbi using cold air dried yellow corvina | |
KR101861009B1 (en) | the braised yellow corvina manufacture method use of extract like that Green tea and Centella asiatica, Lespedeza bicolor | |
KR101212718B1 (en) | Preparing method for soybean sauce using Salicornia herbacea powder by high pressure water extracting | |
KR101448138B1 (en) | Beverage comprising extract of mulberry leaves and herbs | |
KR102509537B1 (en) | Method for Making Seasoned Laver having inhibited acidification and Seasoned Laver manufactured thereby | |
KR101870419B1 (en) | Method for preparing chungkookchang comprising nipa fruticans wurmb and chungkookchang by the method | |
KR102120461B1 (en) | Process for preparing cold noodle with Hippophae rhamnoides | |
KR102120463B1 (en) | Process for preparing kalguksu noodle with Hippophae rhamnoides | |
KR102073393B1 (en) | Methdo for preparing soybean sauce and paste using fermented chaga mushroom powder and bamboo salt | |
KR101211075B1 (en) | Preparing method for soybean paste using Salicornia herbacea powder by high pressure water extracting | |
KR101198416B1 (en) | Preparing method for Salicornia herbacea powder by high pressure water extracting | |
KR101764307B1 (en) | Salt Comprising Extract of Lotus Preparation Method thereof | |
KR101891806B1 (en) | Processing method of meats or fishes comprising nipa fruticans wurmb and meats or fishes by the method | |
KR20180047968A (en) | manufacturing method of fermented soybeans using Dendropanax morbifera | |
KR101891807B1 (en) | Method for manufacturing makgeolli withnipa fruticans wurmb and makgeolli by the method | |
KR101184914B1 (en) | Method for processing fish using blueberry and salted fish, salted mackerel thereof | |
KR101111288B1 (en) | Functional red pepper paste and manufacturing method thereof | |
KR101846941B1 (en) | Feed composition with nipa fruticans wurmb | |
KR100524375B1 (en) | Soybean paste containing a lotus root and a method thereof | |
KR102345350B1 (en) | Bamboo salt comprising dendropanax morbiferus and manufacturing method thereof | |
KR101948919B1 (en) | Natural seasoning with nipa fruticans | |
KR101333575B1 (en) | Bean curd containing ramie leaves improved in shelf-life and method thereof | |
KR102426674B1 (en) | Kadsura coccinea liquid tea and Manufacturing method thereof | |
KR102455930B1 (en) | Process for preparing fermented barley grain and the use thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
A302 | Request for accelerated examination | ||
E902 | Notification of reason for refusal | ||
E90F | Notification of reason for final refusal | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant |