KR20180024421A - Hangover drink comprising desalinized magma seawater and wood extracts and process for the production thereof - Google Patents

Abstract

The present invention relates to a composition for the prevention or relief of hangover comprising a Hovenia dulcis extract, an oak extract, and desalinated magma seawater. The Hovenia dulcis extract, oak extract, and desalinated magma seawater of the present invention can be usefully used for the prevention or relief of hangover by reducing blood alcohol concentration in the human body and alleviating hangover symptoms.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a composition for hanging hull compost including desalted lava sea water and wood extract,

The present invention relates to a composition for prevention or improvement of hangover comprising hinoki extract, oak extract and desalted lava sea water.

Drugs that can remove hangovers due to frequent drinking and frequent alcoholic beverages are highly interested. Unlike other foods, alcohols that can not be stored in tissues are metabolized in the gastrointestinal mucosa by alcohol dehydrogenase (ADH) and microsomal ethanol oxidizing system (MEOS). . The ethanol metabolism in the liver is mainly by NAD-linked enzymes (alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH). These enzymes produce acetaldehyde and acetate, respectively, and acetate is converted to acetyl-CoA to generate energy via TCA circuitry or to synthesize cholesterol and fatty acids. Both ADH and MEOS in the stomach and liver oxidize alcohol to acetaldehyde in the first step, so that the amount of acetaldehyde produced increases as the alcohol is metabolized by these enzymes.

Acetaldehyde binds to important active substances, including proteins, in vivo, causing symptoms such as increased pulse, sweating, nausea, and vomiting. The second step in the metabolism of alcohol is the acetaldehyde dehydrogenase (ALDH) which oxidizes acetaldehyde to acetate to prevent the accumulation of acetaldehyde and its side effects, but over 15% of Koreans have an inactive form of ALDH The above symptoms due to the accumulation of acetaldehyde may occur during drinking.

Hangover refers to headache, diarrhea, anorexia, nausea, vomiting, chills, and cold sweating that occur after drinking alcohol. Objective symptoms include cognitive decline, decreased exercise capacity, hematologic changes, and hormonal changes.

Alcohol is known to have various effects on liver metabolism depending on its intake. Acetaldehyde and NADH produced in the oxidation process are more damaging to hepatocytes than alcohol itself. Alcohol absorbed in the body reacts with NAD ions by ADH (alchol dehydrogenase), converts to NADPH and acetaldehyde, and then reacts with NAD ion again to convert acetaldehyde to NADPH and acetate by aldehyde dehydrogenase (ALDH). Hangover is caused by acetaldehyde, and both ADH and ALDH can be achieved by hangover resolution. There have been many studies to find drugs that can reduce hangover. Recently, researches and experiments on substances that can reduce the toxicity of ethanol or inhibit the expression of toxins have been conducted. As a result, Or a variety of health supplements containing artificial agents are being developed. However, there have not been many cases in which significant hangover resolution effect has been shown yet.

On the other hand, wood, which is a natural biological resource, is a porous biological material containing a large amount of fiber. It is a kind of porous biomaterial such as glucose, which has a pharmacological component catalyzed by the sun by carbon dioxide and water in water, or glutaraldehyde It is a report of biological materials such as polysaccharides and monosaccharides. These components, which are polymeric materials, are decomposed to low molecular weight by the prevailing heat and pressure conditions, and their pharmacological components are known to have many pharmacological components which are effective for the growth of human beings, animals, and even plants.

Lava seawater is an underground seawater flowing into the ground under natural filtration of seawater by a permeable volcanic rock layer. It is mainly buried in the eastern part of Jeju. It flows up to 8km from the coast and is known to pass through the volcanic rock layer. The lava seawater is not mixed with each other due to the difference in density with freshwater groundwater, and exists below the freshwater layer.

Specifically, the eastern part of Jeju Island refers to Gujwa-eup, Seosan-eup, and Jeonseon-myeon in Cheju Island in the administrative district, and "saltwater groundwater" refers to groundwater containing salinity of a certain amount or more. Specifically, (Electrical conductivity of 1,700 μs / cm to 17,350 μs / cm) and brine groundwater (Kwangwon Univ.) In accordance with the criteria for classification of groundwater in Jeju Island, Preferably not less than 17,350 占 / / cm or a salt concentration of 30 ‰ (permilliage) or more (usually, the salt concentration of seawater is 32 ≪ / RTI > to < RTI ID = 0.0 > 35 < / RTI >

Since lava seawater is naturally purified during the passage through the basaltic layer, it is very clean and unlike deep sea water which is taken from deep sea, it is cost effective because it can be taken from the land. Lava seawater produced in Jeju Island is very rare Since it is considered to be very useful for making Jeju Island brand products as a material, it is expected to become a global product for many tourists.

In addition, the lava water contains a large amount of various minerals not found in general salt ground water or seawater. In particular, it is known that vanadium contained in trace amounts in lava seawater has an action of stabilizing insulin secretion, It has been reported that selenium inhibits the oxidation of fat, antioxidant activity, anticancer, infertility, suppression of aging, and improvement of cholesterol level. However, regarding the hangover remedy There is no such thing.

As a result of efforts to solve the hangover, the inventors of the present invention have found that a composition containing an extract of Hovenia dulcis, an extract of oak, and a deionized sea water as an active ingredient decreases the alcohol content in the blood after drinking and the alcohol dehydrogenase and acetaldehyde dehydrogenase activity And the present invention has been completed.

Study of the antioxidant and alcohol-degrading enzyme activities of soybean sprout sugar solutions. Korean J Food Sci Technol. 46 (5): 581-587, Kim KM, Jung HJ, Sung HM, Wee JH, Kim TY and Kim KM (2014) Protective effects of branched-chain amino acid (BCAA) -enriched corn gluten hydrolysates on ethanol-induced hepatic injury in rats. Korean J Food Sci Technol 41: 706-711, Chung YI, Bae IY, Lee JY, Chun HS and Lee HG (2009) Effect of a soy-sprout beverage prepared with high-concentrated oxygen water on alcohol metabolism in rats. Korean J Food Sci Technol 46 (5): 616-621, Sung HM, Jung HJ, Yun SK, Kim TY, Kim KM and Wee JH (2014) Effect of flower of pueraria lobata on lipid peroxidation and activities of alcohol metabolic enzymes in alcohol-treated rats. J Korean Soc Food Sci Nutr 29 (5): 935-942, Lee JS, Kim NY, Lee KH, Kim GS, Park HJ, Choi JW and Kim SH (2000) The effect of mildronate on serum alcohol concentration and hangover syndrome. J Korean Soc Food Sci Nutr 27: 168-174, Park SM, Kang BK and Chung TH (1998) Effect of dandelion juice supplementation on alcohol-Induced oxidative stress and hangover in healthy male college students. J Korean Soc Food Sci Nutr 38 (6): 683-693, Noh KH, Jang JH, Kim JJ, Shin JH, Kim DK and Song YS (2009)

It is an object of the present invention to provide a composition for hunting hunting which comprises Hovenia dulcis extract, oak extract and desalted lava sea water as an active ingredient.

In order to accomplish the above object, the present invention provides a composition for preventing or improving hangover comprising Hovenia dulcis extract, oak extract and desalted lava sea water as an active ingredient.

The present invention also provides a health functional food for prevention or improvement of hangover, which comprises Hovenia dulcis extract, oak extract, and desalted lava sea water as an active ingredient.

In addition, the present invention provides a hangover herb drink containing as an active ingredient a hinoki extract, an oak extract, and desalted lava sea water.

The Hovenia dulcis extract, oak extract and desalted lava sea water of the present invention can be usefully used for prevention or improvement of hangover by reducing blood alcohol concentration in the human body and alleviating hangover symptoms.

FIG. 1 schematically shows a process for producing an oak extract.
FIG. 2 is a graph showing the compositional analysis results of the Hovenia dulcis extract through a GC / MS spectrum.
Fig. 3 is a graph showing the result of analyzing the components of the crabgrass extract through a GC / MS spectrum.
FIG. 4 is a graph showing the result of analyzing the composition of the uroguide extract through a GC / MS spectrum. FIG.
FIG. 5 is a graph showing the compositional analysis results of the Hovenia dulcis extract through a GC / MS spectrum.
6 is a graph showing the results of measurement of acetaldehyde degrading enzyme activity of the composition of the present invention.
FIG. 7 is a graph showing the results of measurement of respiration alcohol concentration in clinical subjects who ingest the composition of the present invention before alcohol consumption. FIG.
FIG. 8 is a graph showing the results of measurement of alcohol concentration during breathing of clinical subjects who ingest the composition of the present invention before alcohol consumption, by discriminating whether or not to smoke.
FIG. 9 is a graph showing the results of measurement of alcohol concentration during breathing of the clinical subjects who ingested the composition of the present invention before alcohol consumption, by sex.

The present invention provides a composition for prevention or improvement of hangover comprising Hovenia dulcis extract, oak extract and desalted lava sea water as an active ingredient.

The composition comprises a pharmaceutical composition or a food composition.

Hereinafter, the present invention will be described in detail.

The extracts of hinoki and oak, which are active ingredients of the present invention, can be obtained by extracting and isolating from wood each using an extraction and separation method known in the art, and the 'extract' as defined in the present invention is suitable Crude extracts extracted using a solvent, polar solvent-soluble extracts, or non-polar solvent-soluble extracts.

The Oriental raisin tree ( Hovenia dulcis ) is a deciduous tree belonging to the family Seagull and is also referred to as a seed tree. The oak refers to a deciduous broad-leaved tree belonging to the oak tree and includes quercus mongolica, oak tree, oak oak, oak tree, oak tree, And may be preferably a Quercus mongolica.

 The hinoki extract and the oak extract may be obtained by extracting from various organs or parts such as leaves, flowers, roots, stems, branches, husks and fruits, but preferably they are obtained from woody parts, Bark). ≪ / RTI >

As a suitable solvent for extraction, any of water or a pharmaceutically acceptable organic solvent may be used. Examples of the solvent include alcohols having 1 to 4 carbon atoms such as water, methanol, ethanol, propanol, isopropanol, and butanol, acetone, ether various solvents such as ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane may be used alone or as a mixture of two or more thereof. And preferably water.

Examples of the extraction method include hot water extraction, cold extraction, reflux cooling, solvent extraction, steam distillation, ultrasonic extraction, elution, and compression.

The desired extract may further be subjected to a conventional fractionation process or may be purified using a conventional purification method. For example, the extract of Hovenia dulcis and the extract of oak, which are effective components of the composition of the present invention, can be prepared into a powdery state by an additional process such as vacuum distillation, freeze-drying, or spray drying. In addition, the primary extract can be further purified into a further purified fraction using various chromatographies such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography and the like Can be obtained.

Therefore, the extract used in the present invention is a concept including all the extracts, fractions and tablets obtained in each step of extraction, fractionation or purification, their diluted solution, concentrate or dried product.

In one embodiment of the present invention, the wood is hydrolyzed with water or an alcohol having 1 to 4 carbon atoms, preferably water, preferably at 50 to 200 DEG C for 0.1 to 3 hours to obtain a hinoki extract and an oak extract, The oak extract was obtained through vacuum concentration.

Specifically, a process of dipping a wooden chip in water and then dehydrating it; Heating the dehydrated wood chips at 70 to 150 DEG C for 0.5 to 3 hours; A step of softening the heated wood chips and water vapor by reacting them at a temperature of 120 to 300 ° C and a pressure of 10 to 40 kgf / cm 2 for 0.1 to 1 hour; Releasing and expanding the softened wood chips into the atmosphere; Subjecting the expanded wood chips to hot water extraction at 50 to 200 DEG C; And a step of separating, purifying and concentrating the extract under reduced pressure.

In addition, the oak extract may further include a step of cutting wood with wood chips to remove bark after the dehydrating process, a step of cutting with a chip maker to produce a wood chip, and a pretreatment step of physical, chemical, and biological pretreatment . The pretreatment step may be explosion, alkali treatment, sulfur dioxide treatment, hydrogen peroxide treatment, supercritical ammonia treatment, weak acid extraction treatment, ammonia explosion, ionic liquid treatment, and the like.

The blowing method is a method of producing a fiber board called "Masonite process" or "gun". It is a method of disassembling a chip by treating a wood chip with high-pressure steam at a high temperature and rapidly discharging it into the atmosphere. The high pressure acts to break down the wood into small pieces.

The hinoki extract and the oak extract may be contained in an amount of 0.01 to 95% by weight, preferably 1 to 80% by weight, more preferably 1 to 20% by weight based on the total weight of the composition. If the content is less than 0.01% by weight, the efficiency of taking may be poor. If the content is more than 95% by weight, formulation may be difficult.

Another effective ingredient of the present invention, "desalted lava sea water" is a result obtained by desalting lava seawater. The resultant may be a partially dehydrated (with some water removed) concentrated liquid phase property or a fully dehydrated powder phase property . This desalted lava sea water can be understood as a composition of mineral components whose phase is not particularly limited.

The above-mentioned "Lava Seawater" is an underground seawater flowing into the ground under natural filtration of seawater by a permeable volcanic rock layer. It is mainly buried in Jeju Island, preferably in the eastern part of Jeju Island (Jeju Island, Seosan-eup and Jeolseon-myeon) It flows up to 8km from the coast and is known to pass through the volcanic rock layer. The lava seawater is not mixed with each other due to the difference in density with freshwater groundwater, and exists below the freshwater layer.

A method of desalting lava sea water to obtain desalted lava sea water can be carried out by applying any technique known in the art, and examples thereof include reverse osmosis method, ion exchange resin method, and electrodialysis method.

In the composition of the present invention, the desalted lava water is the result obtained by desalting lava seawater, and when dehydrated lava lava is used, it is based on desalted lava water to which dehydrated amount of water is added.

In addition, compositions of the present invention mallotus (Mallotus japonicus ) extract.

The yew tree is a deciduous arboreous tree with dicotyledonous deciduous forests and mainly grows on the beach, and can be preferably a mountain of Jeju Island.

The Yedoaceae extract is obtained by extracting from various organs or parts such as leaves, flowers, roots, stems, branches, bark and fruit, and preferably refers to a stem, branch, shell or root, , Bark).

In addition, the composition of the present invention may further comprise an extract of Galapthong, Ganoderma lucidum, Yeoju, Ulugum, Japanese, Punggol, or Scutellaria.

Puerarialo bata is a dried flower of 칡, widely used for thirst, anorexia, abdominal distension, vomiting and so on.

The Houttuynia cordata Thunb) is a perennial herbaceous plant belonging to Saururaceae . It is known to be effective in the treatment of hemostasis, diuretic, diuretic, humoral, swelling, spontaneous, detoxification, bruising, hemorrhage,

The Momordica charantia ) is a vine-grown herbaceous herb that has been widely used for edible, ornamental, and medicinal purposes. It is known to be effective for liver protection, antitumor action, and antioxidant activity.

Curcuma longa L. (turmeric) is a perennial herbaceous herbaceous plant belonging to the genus Ginger and has been used for herbal medicines, spices and edible products, It is known to be effective for mutagenic, anti-inflammatory and antimicrobial properties.

The chair Leaflets are perilla (Perilla frutescens ) is used for sweating, hemostasis, fever, analgesia, sedation and skin diseases. It is also known to be effective for preventing food poisoning and restoring fatigue.

Taraxacum platycarpum has been used as a traditional medicament for the treatment of liver diseases and female diseases and improves liver, pancreas and gastrointestinal function and plays a role of diuretic.

The above-mentioned Cornus walteri wagner belongs to the order Tertiary, and is known to be effective for detoxification, antipyretic action and diuretic action.

The present inventors have confirmed that the composition of the present invention including hinoki extract, oak extract, and desalted lava sea water reduces the blood alcohol concentration in the human body and alleviates the hangover symptoms.

The composition of the present invention may further contain one or more known active ingredients having a hangover resolution effect together with the Hovenia dulcis extract, the oak extract, and the desalted lava sea water.

The compositions of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the manufacture of the compositions. In addition, it can be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, oral preparations such as syrups and aerosols, external preparations, suppositories and sterilized injection solutions according to a conventional method. Suitable formulations known in the art are preferably those as disclosed in Remington ' s Pharmaceutical Science, recently, Mack Publishing Company, Easton PA. Examples of carriers, excipients and diluents which may be included include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, Cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil. When the composition is formulated, it is prepared using a diluent such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, or an excipient usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose, lactose, Gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. As a base for suppositories, witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like can be used.

The term "administering" as used herein is meant to provide any desired composition of the invention to a subject in any suitable manner.

The preferred dosage of the composition of the present invention varies depending on the condition and the weight of the individual, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. For a desired effect, the daily dose of the composition of the present invention may be administered in an amount of 1 mg / kg to 10000 mg / kg, and may be administered once a day or divided into several times.

The compositions of the present invention may be administered to a subject in a variety of routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-dural or intracerebroventricular injection, alone for hangover resolution, or by other methods Can be used together.

Further, the present invention provides a health functional food for prevention or improvement of hangover, comprising Hovenia dulcis extract, oak extract, and desalted lava sea water as an active ingredient.

In addition, the present invention provides a beverage for hangover containing an extract of Hovenia dulcis, an oak extract, and desalted seawater as an active ingredient.

When the hinoki extract, oak extract and desalted lava sea water of the present invention are used for a health functional food or beverage, the hinoki extract, oak extract, and desalted lava sea water can be directly added or used together with other food or food ingredients, Can be suitably used according to the method of The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). Generally, the desalted lava waters of the present invention are added in an amount of not more than 15% by weight, preferably not more than 10% by weight based on the raw material, in the production of food or beverage. However, in the case of long-term intake for the purpose of health and hygiene or for the purpose of controlling health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

There is no particular limitation on the kind of the food. Examples of the foods to which the above substances can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen noodles, gums, ice cream, various soups, drinks, tea, Beverages, and vitamin complexes, all of which include foods in a conventional sense.

The beverage may contain various flavors or natural carbohydrates as an additional ingredient such as a conventional beverage. The natural carbohydrates may be monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, natural sweeteners such as dextrin and cyclodextrin, synthetic sweeteners such as saccharine and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 10 g, preferably about 0.01 to 0.1 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention may further contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, A carbonating agent used in a carbonated beverage, and the like. In addition, the composition of the present invention may comprise flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. Although the ratio of such additives is not critical, it is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, preferred embodiments, experimental examples, and production examples are provided to facilitate understanding of the present invention. However, the following Examples, Experimental Examples and Preparation Examples are provided only for the purpose of easier understanding of the present invention, and thus the present invention is not limited thereto.

Example  One. Hinoki  Preparation of extract

The manufacturing process for preparing Hovenia dulcis extract is schematically shown in Fig.

As shown in Fig. 1, a hippocampus wood was clipped and peeled to remove bark, and then cut with a chip maker to produce a wood chip. Then, the wood chips were impregnated in water of 5 to 20 times volume, impregnated for 10 to 48 hours, dehydrated, and the dehydrated wood chip was heated at a temperature of 70 to 150 ° C for 30 minutes to 3 hours I turned it on. The heated wood chip was placed in a sealed container together with water vapor and maintained in a saturated state and softened at a temperature of 120 to 300 ° C and a pressure of 10 to 40 kgf / cm 2 for 0.1 to 1 hour. The softened wood chips were then released into the atmosphere to expand the wood chips and the expanded wood chips were extracted in a 5 to 20 times volume of water in a hot water extractor at 50 to 200 DEG C for 0.1 to 3 hours. The residue and the filtrate produced in the extraction process were separated using a centrifuge, and the residue was removed by filtration using a filter paper. The residue was dissolved in a rotary vacuum concentrator (Rotavapor R-205, BUCHI, Postfach, Switzerland ) Under reduced pressure.

Example  2. Manufacture of oak extract

The manufacturing process for producing the oak extract is schematically shown in Fig.

As shown in Fig. 1, the oak timber was cut to remove the bark, followed by cutting with a chip maker to produce a wood chip. Then, the wood chips were impregnated in water of 5 to 20 times volume, impregnated for 10 to 48 hours and dehydrated. The dehydrated wood chips were heat-treated at a temperature of 70 to 150 ° C for 30 minutes to 3 hours Lt; / RTI > The heated wood chip was placed in a sealed container together with water vapor and maintained in a saturated state and softened at a temperature of 120 to 300 ° C and a pressure of 10 to 40 kgf / cm 2 for 0.1 to 1 hour. The softened wood chips were then released into the atmosphere to expand the wood chips and the expanded wood chips were extracted in a 5 to 20 times volume of water in a hot water extractor at 50 to 200 DEG C for 0.1 to 3 hours. The residue and the filtrate produced in the extraction process were separated using a centrifuge, and the residue was removed by filtration using a filter paper. The residue was dissolved in a rotary vacuum concentrator (Rotavapor R-205, BUCHI, Postfach, Switzerland ) Under reduced pressure.

Example  3. Jeju Lava  Component analysis

In order to confirm the constituents of Jeju lava sea water which is an effective ingredient of the present invention, lava seawater was obtained from Gujwa-eup, Bukjeju-gun, eastern Jeju Island, and then the quality of water to be inspected was measured using an inductively coupled plasma atomic emission spectrometer (ICP spectrophotometer) (Mg), sodium (Na), potassium (K), phosphorus (P), copper (Cu), zinc (Zn), iron (Fe), manganese ), Cadmium (Cd), aluminum (Al) and arsenic (As) were investigated. At this time, general tap water was used as a control group, and the results are shown in Table 1 below.

Constituent Lava seawater tap water Calcium (Ca) 13.35 2.86 Magnesium (Mg) 1179.46 5.52 Sodium (Na) 606.31 16.34 Potassium (K) 209.48 21.63 In (P) ND ^* N.D. Copper (Cu) N.D. N.D. Zinc (Zn) N.D. N.D. Iron (Fe) N.D. N.D. Manganese (Mn) N.D. N.D. Lead (Pb) N.D. N.D. Cadmium (Cd) N.D. N.D. Aluminum (Al) N.D. N.D. Arsenic (As) N.D. N.D.

Unit: ppm (mg / L), * N.D is less than or equal to 0.1 ppm

As shown in Table 1, the content of minerals such as calcium, magnesium, sodium and potassium was significantly higher in lava seawater than tap water. Calcium of Jeju lava seawater was 4.7 times, magnesium was 214 times, sodium was 37 times, and potassium was 10 times higher than tap water. It was estimated that the tap water was highly refined while passing through the water treatment plant, while the lava water was estimated to have a very high mineral content due to the characteristics of the seawater as the raw water. Heavy metal components were not detected in both lava seawater and tap water.

In addition, the lava water was desalted for use in the present invention, and the desalted lava waters obtained through the above procedure were used in the following experiments.

Example  4. Hinoki  Analysis of components of extract

A gas chromatograph mass spectrometer (GC / MS) (Agilent, 7890A-5975C GC / MSD) was used to identify the components of the Hovenia dulcis extracts prepared in Example 1. At this time, DB-5 ms (30 m × 250 m × 0.25 m) was used as the column, and the temperature of the column was raised from 50 ° C. to 300 ° C. at a rate of 5 / min. MSD (Mass Selective Detector) was used as the detector, and the results are shown in Table 2 and FIG.

Retention
time (min) Compound Peak
area (%) Molecular formula 7.00 Hydroxyacetone; 2-Propanone, 1-hydroxy- 8.13 C ₃ H ₆ O ₂ 7.29 Methyl formate; Formic acid, methyl ester 4.77 C ₂ H ₄ O ₂ 9.77 Acetic acid 17.78 C ₂ H ₄ O ₂ 10.97 Formic acid 4.54 HCOOH 11.30 1,3-Butanediol 0.68 C ₄ H ₁₀ O ₂ 11.37 Propanoic acid 0.99 C ₃ H ₆ O ₂ 11.94 2,3-Butanediol 0.43 C ₄ H ₁₀ O ₂ 13.00 Butyrolactone 0.47 C ₄ H ₆ O ₂ 13.44 Furfuryl alcohol; 2-Furanmethanol 1.04 C ₅ H ₆ O ₂ 15.23 2-Hydroxy-2-cyclopenten-1-one 1.90 C ₅ H ₆ O ₂ 19.16 Furaneol 1.73 C ₆ H ₈ O ₃ 22.33 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl- 2.54 C ₆ H ₈ O ₄ 22.81 Diglycerol 1.93 C ₆ H ₁₄ O ₅ 25.18 5-Hydroxymethylfurfural 1.40 C ₆ H ₆ O ₃ 34.03 21-CROWN-7; 1,4,7,10,13,16-Hexaoxacyclooctadecane 4.66 C ₁₄ H ₂₈ O ₇

As shown in Table 2 and Fig. 2, acetic acid showed the highest peak area, and furaneol was also identified as a volatile flavor component.

Example  5. Component analysis of additional plant extracts

The components of Galviniae, Ulmus, Lycaeus, and Pseudomonas aeruginosa were analyzed using the same method as in Example 4 above.

4-1. Gall

The results of the GC / MS analysis of gallstone are shown in Table 3 and FIG.

Retention
time (min) Compound Peak
area (%) Molecular formula 7.03 Hydroxyacetone; 2-Propanone, 1-hydroxy- 3.05 C ₃ H ₆ O ₂ 9.80 Acetic acid 20.99 C ₂ H ₄ O ₂ 13.45 Furfuryl alcohol; 2-Furanmethanol 0.63 C ₅ H ₆ O ₂ 15.24 2-Hydroxy-2-cyclopenten-1-one 5.10 C ₅ H ₆ O ₂ 16.69 Guaiacol; Phenol, 2-methoxy- 1.19 C ₇ H ₈ O ₂ 17.70 Acrylamide 0.74 C ₃ H ₅ NO 18.20 Maltol 1.26 C ₆ H ₆ O ₃ 18.63 Glutaconic anhydride; 2H-Pyran-2,6 (3H) -dione 1.02 C ₅ H ₄ O ₃ 21.45 2-Methoxy-4-vinylphenol 0.96 C ₉ H ₁₀ O ₂ 22.34 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl- 3.44 C ₆ H ₈ O ₄ 22.82 Glycerin 3.50 C ₃ H ₈ O ₃ 24.48 Benzoic acid 6.80 C ₇ H ₆ O ₂ 32.40 21-CROWN-7 5.20 C ₁₄ H ₂₈ O ₇

As shown in Table 3 and FIG. 3, acetic acid, guaiacol, maltol and the like were confirmed.

4-2. Kool

The GC / MS analysis results of Ulugum are shown in Table 4 and FIG.

Retention
time (min) Compound Peak
area (%) Molecular formula 5.06 Eucalyptol 1.36 C ₁₀ H ₁₈ O 7.02 Hydroxyacetone; 2-Propanone, 1-hydroxy- 6.54 C ₃ H ₆ O ₂ 9.80 Acetic acid 23.69 C ₂ H ₄ O ₂ 11.31 2,3-Butanediol 2.93 C ₄ H ₁₀ O ₂ 11.95 2,3-Butanediol, [R- (R *, R *)] - 8.83 C ₄ H ₁₀ O ₂ 15.24 alpha-Angelica lactone 3.14 C ₅ H ₆ O ₂ 32.40 21-CROWN-7; 1,4,7,10,13,16-Hexaoxacyclooctadecane 13.90 C ₁₄ H ₂₈ O ₇

As shown in Table 4 and Fig. 4, eucalyptol and the like were confirmed.

4-3. Yeoju

The GC / MS analysis results of Yeoju are shown in Table 5 and FIG.

Retention
time (min) Compound Peak
area (%) Molecular formula 7.02 Hydroxyacetone; 2-Propanone, 1-hydroxy- 3.36 C ₃ H ₆ O ₂ 9.80 Acetic acid 14.37 C ₂ H ₄ O ₂ 11.01 Formic acid 4.62 HCOOH 13.00 Butyrolactone 0.79 C ₄ H ₆ O ₂ 13.44 Furfuryl alcohol; 2-Furanmethanol 1.56 C ₅ H ₆ O ₂ 15.23 2-Hydroxy-2-cyclopenten-1-one 38.67 C ₅ H ₆ O ₂ 18.63 Glutaconic anhydride; 2H-Pyran-2,6 (3H) -dione 2.67 C ₅ H ₄ O ₃ 19.90 Dihydroxyacetone; 1,3-Dihydroxyacetone dimer 8.21 C ₃ H ₆ O ₃ 20.28 Octane 2.27 C ₈ H ₁₈ 22.34 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl- 3.52 C ₆ H ₈ O ₄ 26.31 18-CROWN-6; 1,4,7,10,13,16-Hexaoxacyclooctadecane 1.02 C ₁₂ H ₂₄ O ₆ 32.40 21-CROWN-7; 1,4,7,10,13,16-Hexaoxacyclooctadecane 13.90 C ₁₄ H ₂₈ O ₇

4-4. POE

The results of GC / MS analysis of POCs are shown in Table 6 below.

Retention
time (min) Compound Peak
area (%) Molecular formula 7.02 Hydroxyacetone; 2-Propanone, 1-hydroxy- 10.25 C ₃ H ₆ O ₂ 9.79 Acetic acid 26.19 C ₂ H ₄ O ₂ 11.07 Formic acid 3.81 HCOOH 12.42 Octamethylsilsesquioxane;
Pentacyclo [9.5.1.1 (3,9) .1 (5,15) .1 (7,13)] octacyloxane, octamethyl- 0.99 C ₈ H ₂₄ O ₁₂ Si ₈ 15.23 2-Hydroxy-2-cyclopenten-1-one 2.54 C ₅ H ₆ O ₂ 22.33 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl- 2.42 C ₆ H ₈ O ₄ 22.81 Glycerin 7.20 C ₃ H ₈ O ₃ 32.40 21-CROWN-7; 1,4,7,10,13,16-Hexaoxacyclooctadecane 20.64 C ₁₄ H ₂₈ O ₇

Example  5. Preparation of mixed drinks 1

The hinoki extracts and oak extracts prepared in Examples 1 and 2 were dried in a freeze dryer at -85 캜 to prepare powdered beverages, and mixed with the desalted lava waters obtained in Example 3 to prepare mixed beverages.

Example  6. Manufacturing of mixed drinks 2

The hinoki extracts and oak extracts prepared in Examples 1 and 2 were dried at -85 ° C in a freeze dryer to prepare powdery form. The extracts were prepared by extracting the extracts of Yeduckweed, Ganoderma lucidum, Yeoju, The extracts were also prepared in powder form in the same manner and mixed with the desalted lava waters obtained in Example 3 to prepare mixed beverages.

Experimental Example  1. Mixed drinks ALDH ( aldehyde dehydrogenase ) Active measurement

The activity of the aldehyde dehydrogenase (ALDH) of the mixed beverage of the present invention was measured according to the recommended procedure of the kit using an acetaldehyde assay kit (Megazyme Co., Ltd., Wicklow, Germany). First, 0.2 mL of sodium azide buffer (pH 9.0) and 0.2 mL of NAD ⁺ were added to 2.1 mL (100 mg / mL) of the mixed drink prepared in Example 5, and the mixture was reacted for 2 minutes. Absorbance was measured at 340 nm using a photometer (A1). After that, 0.05 mL of ALDH enzyme was added and reacted for 3 ~ 4 minutes. Absorbance was measured at 340 nm using a UV-spectrophotometer (A2). At this time, distilled water was used as a control group, and the activity of ALDH was calculated by the following equation, and the result is shown in FIG.

As shown in FIG. 6, the activity of ALDH in the control group was 100%. The activity of ALDH in control group was 111%, 224%, 202%, 129%, 232%, 114% and 290% As a result, it was confirmed that the mixed beverage of the present invention exhibited significantly higher ALDH activity as compared with the control group. In particular, the mixed beverage according to the present invention exhibited an activity of about twice or more as much as that of gall bladder, myofibroblast and scabbard, and thus it was considered to be effective in eliminating ALDH and hangover.

Experimental Example  2. Clinical trial of hangover effect of mixed drinks

In order to clinically confirm the hangover resolution effect of the mixed beverage of the present invention, the alcohol concentration during respiration was measured using a commercially available respiratory alcohol meter (DA-5000, Datech Co., Korea). For the clinical experiment, 16 college students who enjoyed drinking with more than 1 bottle of soju and interested in study and participated in the study were selected as subjects for the study. Park et al (1998 The experimental method was applied.

First, the subjects were asked to eat light meals two hours before the beginning of drinking to resemble the food intake before drinking, and they started drinking at 7 pm. The experimental group was divided into two groups. In the control group, 200 mL of water was consumed 30 minutes before drinking. In the group of hangover drink group, 200 mL of the mixed drink prepared in Example 5 was consumed. After 30 minutes of drinking each sample, 360 mL of alcohol (16.5%) was divided into portions at a constant rate within 30 minutes. All the subjects were allowed to smoke during the experiment, could not drink water, and allowed the restroom to freely enter and exit. After that, the alcohol concentration was measured at 30 minutes, 60 minutes, 90 minutes and 120 minutes from the end of drinking, and the results are shown in FIG.

As shown in FIG. 7, after 30 minutes of alcohol consumption, 0.084% in the control group, 0.059% in the hangover solution mixed drink group, 0.068% in the control group after 60 minutes, 0.053% in the hangover solution mixed drink group, , 0.048% in the control group, 0.065% in the hangover drink mixed drink group, 0.068% in the control group after 120 minutes, and 0.048% in the hangover drink mixed drink group, indicating lower alcohol concentration in the hangover drink mixed drink group Respectively.

The alcohol concentration in the respiration of the group of drinkers with hangover elimination mixed drink was divided into the presence or absence of smoking and the result was shown in FIG.

As shown in FIG. 8, 0.055% in the non-smoker hangover elimination drink group, 0.047% in the smoker hangover elimination drink group, 0.050% in the non-smoker hangover elimination drink group after 60 minutes, Smokers hangover resolution 0.047% in mixed drink group, 0.050% in non-smoker hangover elimination group 0.050% in smoking group, 0.050% in smokers in mixed drink group, non-smoker hangover in mixed drink group , 0.025%, and 0.043% in smokers' hangover elimination mixed drink group, respectively. There was no significant difference in alcohol concentration according to nonsmokers and smokers. However, after 120 minutes, it was lower in non - smoker hangover drink drink group than non - smoker hangover drink drink group. Therefore, non - smoker was more effective than non - smoker drinking hangover drink.

In addition, the alcohol concentration in the respiration of the hangover-eliminating mixed drink group was measured by sex, and the results are shown in FIG.

As shown in FIG. 9, 0.037% in male drink hangover drink drink group, 0.050% in female hangover drink drink group, 0.060% in male hangover drink drink group after 60 minutes, and 0.060% in female drink hangover drink drink group 0.048% in drink ingestion group, 0.090% in male drink hanging drink drink group, 0.050% in female hangover drink drink group, 0.067% in male hangovers drink drink group, 0.048% in female drink hanging drink drink group , And 0.036% in the group with alcoholic beverages, while alcoholic beverages showed no tendency to change the alcohol concentration with time, but alcoholic beverages had a tendency to decrease steadily over time , 90 minutes, and 120 minutes, the alcohol concentration was lower than that of the male hangover elimination drink group, and the intake of hangover drink was more effective for the female than the male It was considered.

According to the above results, it was found that the Hovenia dulcis extract, oak extract and desalted lava sea water of the present invention can be effectively used as a composition for preventing or improving hangover by reducing blood alcohol concentration in the human body and alleviating hangover symptoms.

Hereinafter, formulation examples of the pharmaceutical composition and the food composition of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

Formulation example  1. Pharmaceutical preparations

1-1. Manufacture of Powder

20 mg of the composition of the present invention

Lactose 100 mg

Talc 10 mg

The above components are mixed and filled in airtight bags to prepare powders.

1-2. Manufacture of tablets

10 mg of the composition of the present invention

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

1-3. Preparation of capsules

10 mg of the composition of the present invention

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

1-4. Injection preparation

10 mg of the composition of the present invention

180 mg mannitol

Sterile sterilized water for injection 2974 mg

Na ₂ HPO ₄ 2H ₂ O 26 mg

(2 ml) per 1 ampoule in accordance with the usual injection preparation method.

1-5. Manufacture of liquid agent

20 mg of the composition of the present invention

10 g per isomer

5 g mannitol

Purified water quantity

Each component was added and dissolved in purified water according to the usual liquid preparation method, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and purified water was added thereto. The whole was added with purified water to adjust the total volume to 100 ml, And sterilized to prepare a liquid preparation.

Formulation example  2. Food preparation

2-1. Manufacture of health food

100 mg of the composition of the present invention

Vitamin mixture quantity

70 g of vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

0.15 mg of vitamin B2

Vitamin B6 0.5 mg

0.2 g of vitamin B12

Vitamin C 10 mg

Biotin 10 g

Nicotinic acid amide 1.7 mg

Folate 50 g

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

2-2. Health drink  Produce

100 mg of the composition of the present invention

Vitamin C 15 g

Vitamin E (powder) 100 g

19.75 g of ferrous lactate

3.5 g of zinc oxide

Nicotinic acid amide 3.5 g

Vitamin A 0.2 g

Vitamin B1 0.25 g

Vitamin B2 0.3 g

Water quantity

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 DEG C for about 1 hour. The solution thus prepared was filtered and sterilized in a sterilized 2 liter container, It is used in the production of the health beverage composition of the invention.

Although the compositional ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the compounding ratio according to the regional or national preference such as the demand class, the demanding country, and the use purpose.

Claims (14)

A composition for preventing or improving hangover, comprising a hinoki extract, an oak extract and a desalted lava sea water as an active ingredient. The composition for preventing or improving hangover according to claim 1, wherein the oak is a Quercus mongolica, an Oak tree, a Quercus variabilis, a Quercus variabilis, an Quercus variabilis or a Quercus variabilis. The composition for preventing or improving hangover according to claim 1, wherein the hinoki and oak are woody. The composition for preventing or improving hangover according to claim 1, wherein the hinoki extract or the oak extract is extracted with a solvent selected from water, an alcohol having 1 to 4 carbon atoms or a mixed solution thereof. The composition for prevention or improvement of hangover according to claim 1, wherein the hinoki extract or the oak extract is a hot-water extract. The method according to claim 1, wherein the oak extract
(a) dewatering the wood chips after immersing them in water;
(b) heating the dehydrated wood chips at 70 to 150 DEG C for 0.5 to 3 hours;
(c) softening the heated wood chips and water vapor by reacting them at a temperature of 120 to 300 ° C and a pressure of 10 to 40 kgf / cm 2 for 0.1 to 1 hour;
(d) releasing and expanding the softened wood chips into the atmosphere;
(e) subjecting the expanded wood chips to hot water extraction at 50 to 200 DEG C; And
(f) a step of separating, purifying and concentrating the extract under reduced pressure. The composition for preventing or improving hangover according to claim 1, wherein the lava seawater is obtained in Jeju Island. The composition for preventing or improving hangover according to claim 1, wherein the desalted lava waters are desalted lava waters by reverse osmosis, ion exchange resin or electrodialysis. [3] The composition for preventing or improving hangover according to claim 1, wherein the hinoki extract and the oak extract are contained in an amount of 1 to 20% by weight based on the total weight of the composition. The composition for preventing or improving hangover according to claim 1, wherein the composition further comprises a mallow extract. 11. The composition for prevention or improvement of hangover according to claim 10, wherein the maltodextrin is Jeju acid. [3] The composition for preventing or improving hangover according to claim 1, wherein the composition further comprises at least one extract selected from the group consisting of Galvinia, Rhizoma, Rhizoma, Lilium, Liliaceae, Pseudomonas spp. A health functional food for prevention or improvement of hangover which contains hinoki extract, oak extract and desalted lava sea water as active ingredients. Hovenia dulcis extract, oak extract, and desalted lava water.

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