KR20180003844A - Pharmaceutical Composition Having High Antibacterial Activity By Bioconversion Of Ulmus davidiana var. japonica Extract and Method For Manufacturing the same - Google Patents

Abstract

The present invention relates to antibacterial activity improvement. More specifically, the present invention relates to a bioconversion Ulmus davidiana var. japonica extract having excellent antibacterial activity through a bioconversion process even if antibacterial activation is hardly present in a state of an Ulmus davidiana var. japonica extract, and a manufacturing method thereof.

Description

 FIELD OF THE INVENTION The present invention relates to a biologically active elm extract having high antimicrobial activity, a process for producing the same, and a pharmaceutical composition comprising the extract. japonica Extract and Method for Manufacturing same same}

The present invention relates to a technology related to the enhancement of antibacterial activity, more specifically, a method for producing the biologically converted elm tree extract which has little antimicrobial activity in the elm extract but has excellent antimicrobial activity through a bioconversion process, ≪ / RTI >

Infectious diseases are collectively referred to as infectious diseases. Infectious diseases means that the pathogenic microorganisms enter and propagate in the living body. In addition, the infection caused by the bacterial proliferation in vivo is caused by the host resistance and pathogenicity It is known that it relies on the mutual relationship of microorganisms with their power. This mutual relationship is mainly caused by normal flora (normal flora), which means a microorganism that harmonizes between hosts. Normal bacterial flies act as host defense mechanisms. Among these microorganisms are beneficial bacteria known to be beneficial to host animals such as lactic acid bacteria, and harmful bacteria which are known to be potentially harmful to hosts such as Escherichia coli, Salmonella, and Staphylococcus. Changes in the microbial flora of the host animal may be altered and destroyed by increased stress, infection by harmful bacteria, and changes in the external environment. In this case, the health of host animals deteriorates due to the rapid growth of harmful microorganisms. They prevent external pathogens from colonizing the skin or intestines and first place them on the skin to prevent growth and infection of other microorganisms.

The technology related to the enhancement of the antibacterial activity of domestic lactic acid bacteria is known by measuring the antibacterial activity of the lactic acid bacteria by isolating the lactic acid bacteria or by improving the antibacterial ability of the lactic acid bacteria using the bioconversion fermentation broth. In addition, the biotransformation technology using herbal medicines is relatively small. However, in the case of bioconversion using the herbal medicine extract, the useful components are extracted and the antibacterial power of the herbal medicine extract and the antibacterial activity by the bacteriocin produced by the lactic acid bacteria This can be seen as an effect of increasing the antibacterial activity.

As a patent for antibacterial activity using lactic acid bacteria, lactic acid bacteria Lactococcus lactis BH5 (KCM 10275), which produces an antimicrobial peptide substance of Patent No. 10-0413335-0000, and novel Lactobacillus bacteria of patent No. 10-1378673-0000, There are patents related to flutamide, a method for producing the same, and a composition for preventing or treating Escherichia coli diarrhea using the same. Examples of patents relating to biotransformation technology using lactic acid bacteria include Patent No. 10-1506995-0000, A patent on a cosmetic composition made of fermented water of Patent No. 10-1051535-0000, and the like.

Patent patents relating to antimicrobial activity using herbaceous plants include Patent No. 10-2006-0060906, a method for producing an antimicrobial shampoo having an antimicrobial activity against Staphylococcus aureus, a shampoo composition prepared by the method, and a method for producing a shampoo composition according to Patent No. 10-0524291-0000 A patent for a cosmetic composition containing a black rice, a black soybean, a black sesame, a mother wolf, an iris, a tea leaf, and a mixed extract of marigold.

The elm (Ulmus davidiana var. Japonica) is a deciduous broad-leaved arboreous tree belonging to the elk and elm in the dicotyledonous plant. It grows well in moist soil, and there are about 6 species in Korea and about 20 species in the world . In spring, young leaves are used for edible purposes, and the skin is called epidermis, and especially the root skin is called epimedium. Among 6 kinds of elm trees which are native to Korea, fruit trees of ripe and juvenile trees are ripened in September and October, and elm elk, elm elk, and elm trees ripen in April and May. These trees differ only in the appearance of the time of ripe berries and the appearance of the shells, but they are the same as leaves and medicines.

Elm has long been known to be effective for boils and swellings. Recently, there have been reports of antioxidative, nitrite removal, anti-bacterial and anti-cancer effects on dental caries. In Donguibogam, elm trees are plain, flavorless, poisonous, and soft, so that they can easily pass through the feces, eliminate the intestines and upper quadrats, become effective in enteritis, calm the swollen and relieve insomnia.

As such, elm contains various useful substances such as flavonoid, saponin, tannin, and mucus, and is used in the treatment of diseases such as allergic rhinitis, asthma, atopy, inflammation, gastric cancer, edema and mastitis in herbal medicine. It is characteristic that it is not toxic to human body.

However, there has been no report that the elm extract has antimicrobial properties against pathogenic skin diseases. Especially, the antimicrobial properties of biosynthesized elm extracts against pathogenic skin diseases caused by lactic acid bacteria have not been reported

The inventors of the present invention completed the present invention by developing a biodegradable elm extract having an antibacterial activity against pathogenic skin disease causative microorganisms in the elm extract but having an excellent antimicrobial activity against causative bacteria of pathogenic skin diseases through a biological conversion process.

Accordingly, an object of the present invention is to provide a biodegradable elm extract having excellent antimicrobial activity against pathogenic skin disease causative bacteria obtained by biotransformation of elm extract having no antibacterial activity against pathogenic skin disease causative bacteria.

It is another object of the present invention to provide a method for preventing pathogenic skin diseases, which comprises administering a specific microorganism capable of imparting excellent antimicrobial activity to pathogenic skin disease bacteria to an elm extract having almost no antibacterial activity against pathogenic skin diseases, And to provide a production method capable of producing a biodegradable elm tree extract having an excellent antibacterial activity against a skin disease causative microorganism.

It is another object of the present invention to provide a variety of application products such as a pharmaceutical composition containing a biosensitive elm extract having excellent antimicrobial activity against pathogenic skin diseases.

The objects of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description.

In order to achieve the object of the present invention described above, the present invention provides a biodegradable elm tree extract having antimicrobial activity against a skin disease causative microorganism.

In a preferred embodiment, the antimicrobial activity is imparted through the biotransformation of the elm extract of the elm.

In a preferred embodiment, the skin disease causative microorganism is at least one selected from the group consisting of purulent Streptococcus pyogenes , Propionibacterium acnes , and Streptococcus pyogenes .

In a preferred embodiment, the lactic acid bacteria used for said lactic acid bioconversion are It is Lactobacillus plantaurum .

In a preferred embodiment, the Lactobacillus plantaurm is L. plantarum 299V (DSM 9843).

The present invention also relates to a method for preparing an elm extract, comprising: preparing a medium for preparing an elm extract-containing liquid medium by adding the elm extract to a lactic acid bacterium culture composition; An inoculation step of inoculating the elm extract-containing liquid medium with the lactic acid bacteria; And culturing the biologically transformed broth by culturing the lactic acid bacterium so as to carry out bioconversion of the elm extract in the inoculated state.

In a preferred embodiment, the method further comprises a removing step of removing the lactic acid bacteria contained in the bioconversion culture.

In a preferred embodiment, the method further comprises a step of drying the bioconversion culture obtained in the cultivation step, or the step of removing the lactic acid bacteria contained in the bioconversion culture and the step of drying the bioconversion culture in which the obtained lactic acid bacteria have been removed .

In a preferred embodiment, the lactic acid bacteria inoculated in said inoculation step is Lactobacillus plantaurum .

In a preferred embodiment, the Lactobacillus Planta room (Lactobacillus plantaurm) is Lactobacillus plantaurm is L.plantarum 299V (DSM9843) and is inoculated in an amount of 1X10 ³ CFU / ml to 1X10 ⁸ CFU / ml.

In a preferred embodiment, the culturing step is carried out at a temperature range of 25 to 40 degrees for 12 to 96 hours.

The present invention also relates to a method for the prevention and / or prevention of an infectious disease caused by pathogenic skin disease bacteria comprising the above-mentioned biodegradable elm extract or the biodegradable elm extract prepared by any one of the above- There is provided a pharmaceutical composition for improving or treating diseases.

In a preferred embodiment, the disease caused by pathogenic skin disease bacteria is one or more of laryngitis, tonsillitis, scarlet fever, hunger, sepsis, acne, sacchoidosis, uveitis, conjunctivitis, keratitis, skin rash, chronic inflammation and pyogenic infection to be.

The present invention also relates to a method for producing a biologically active elm extract of any one of the above-mentioned biotransformed elm or a biologically active elm extract prepared by any one of the above-mentioned methods for producing a health functional food having antimicrobial activity against pathogenic skin diseases .

The present invention also provides a method for treating an infectious disease caused by pathogenic skin disease causing bacteria, comprising the step of administering the above-mentioned pharmaceutical composition to a mammal other than a human.

The present invention also relates to a cosmetic composition having antimicrobial activity against a pathogenic skin disease causative microorganism containing any one of the biodegradable elm extracts described above or the biodegradable elm extract prepared by any one of the above-mentioned manufacturing methods as an active ingredient to provide.

First, the biologically transformed elm extract of the present invention has an excellent antimicrobial activity against pathogenic skin disease causative bacteria.

In addition, the method for producing a biologically-transformed elm extract of the present invention is a method for producing a biologically-transformed elm extract, which comprises administering a specific microorganism to an elm extract having almost no antibacterial activity against pathogenic skin disease causative bacteria, By culturing under culturing conditions, a biodegradable elm tree extract having excellent antimicrobial activity against pathogenic skin diseases can be produced.

In addition, the various application products such as the pharmaceutical compositions including the biodegradable elm extract of the present invention include a biodegradable elm extract having excellent antimicrobial activity against pathogenic skin diseases, thereby preventing the diseases caused by pathogenic skin diseases , Has excellent antimicrobial activity for improvement and treatment.

Figure 1 is a photograph of elm.
FIG. 2 is a graph showing pH changes of the elm extract and the biosynthesis elm extract obtained in the examples of the present invention.
FIG. 3 is a graph showing antimicrobial activity against the skin disease causative microorganism of the elm extract and the biosensitive elm extract obtained in the examples of the present invention.
Fig. 4 is a photograph showing the antimicrobial activity of the elm extract and the biodegradable elm extract obtained in the examples of the present invention against the skin disease causative bacteria.

Although the terms used in the present invention have been selected as general terms that are widely used at present, there are some terms selected arbitrarily by the applicant in a specific case. In this case, the meaning described or used in the detailed description part of the invention The meaning must be grasped.

Hereinafter, the technical structure of the present invention will be described in detail with reference to the accompanying drawings and preferred embodiments.

However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Like reference numerals used to describe the present invention throughout the specification denote like elements.

The technical feature of the present invention is that there is little antimicrobial activity against pathogenic skin disease bacteria in the elm extract, but the biodegradable elm extract obtained through the bioconversion process has an excellent antimicrobial activity against pathogenic skin disease causative bacteria Which has excellent antimicrobial activity against causative bacteria of pathogenic skin diseases, and a method for producing the same. Also, the present invention provides a pharmaceutical composition, a health functional food, and a cosmetic composition which can prevent, improve or treat various infectious diseases by using characteristics having an excellent antimicrobial activity against pathogenic skin disease bacteria that can not be obtained by simple elm extract.

Here, bioconversion refers to a technique of producing and manufacturing various metabolites using enzymes or microorganisms, and it is possible to produce a useful substance by fermentation under anaerobic conditions or micro-aerobic conditions of microorganisms It is the technology that changes. Bioconversion is being carried out using various materials such as plant, herbal medicines, feed, and silk. It is known that there are various effects of biochemical conversion of herbal medicine using microorganisms. Microbial biotransformation is a characteristic feature of the decrease in the size of the useful substance and the improvement of the function. Production of useful substances such as vitamins, organic acids and bacteriocins produced by the microorganisms also serves as an advantage of biotransformation.

Therefore, the biologically-transformed elm extract of the present invention has antimicrobial activity against a causative agent of skin diseases.

The causative bacteria of skin diseases may be one or more of purulent Streptococcus pyogenes , Propionibacterium acnes , and Streptococcus pyogenes , which are known to be causative agents of skin diseases. Streptococcus pyogenes is known to be a cause of human laryngitis, tonsillitis, scarlet fever, hunger, and sepsis. Propionibacterium acnes is a pathogenic bacterium present in the skin and intestinal tract. It causes acne, squidoemia, uveitis, conjunctivitis and keratitis. It causes skin rash and chronic inflammation. Staphylococcus aureus is an important cause of skin infection in atopic patients. It is characterized by the secretion of toxins that kill phagocytes such as hemolysin, coagulase, and lucosidine, leading to a pyogenic infectious disease. Propionibacterium acnes exists in the skin or sebaceous glands and is characterized by massive proliferation when the sebaceous glands are active due to increased adolescent hormone activity. When acne is caused by Propionibacterium acnes in adolescent atopic patients, symptoms such as dry skin and increased skin erythema accompanied by itching will appear. In addition, Staphylococcus aureus and Streptococcus pyogenes The symptoms of acne due to tea bacterium infection worsen. According to Yoon et al. (2009), secondary infection of Streptococcus pyogenes was found in atopic patients with fever, purulent exudates, pustules, pustules, etc. and symptoms of sepsis due to bacterial infection were confirmed. In addition, antibiotics, antihistamines, and topical steroids are used to prevent secondary bacterial infections. Streoid phobia causes side effects of steroids. It is known that Staphylococcus aureus is not only the primary cause of atopic dermatitis but also a secondary cause of infections such as skin pore infection, sweat gland blockage, and wound infection. It is known that 90% Infection of S. aureus has been confirmed. In addition, it suggests that using Oriental medicine can inhibit secondary infection of Streptococcus pyogenes and Staphylococcus aureus .

The antimicrobial activity of the biosynthesized elm extract of the present invention is imparted through the biotransformation of the elm berry extract, and the lactic acid bacterium used for the biosynthesis of the lactic acid bacteria may be Lactobacillus plantaurum . In particular, L. plantarum 299V (DSM 9843), L. plantarum 299V was first isolated in fermented food in 1994, and has been studied in irritable bowel syndrome, mucosal inflammation and the like.

The pH of the biologically-transformed elm extract of the present invention is 4.5 or less, which is compared with the pH of the elm extract before the bioconversion of pH 6 or more, .

Next, the method for producing a biosynthesized elm tree extract of the present invention comprises: preparing a medium for preparing an elm extract-containing liquid medium by adding an elm extract to a lactic acid bacteria culture composition; And an inoculation step of inoculating the elm extract-containing liquid medium with lactic acid bacteria; And culturing the cultured lactic acid bacterium so as to carry out bioconversion of the elm extract. The method of the present invention includes a step of removing a lactic acid bacterium contained in a bioconversion culture, if necessary, Drying the transformed culture, removing the lactic acid bacteria contained in the biotransformation culture, and drying the biotransformation culture from which the obtained lactic acid bacteria have been removed.

Inoculation step is Lactobacillus Lactobacillus plantaurm of Planta room (Lactobacillus plantaurm) can be carried out by inoculating the L.plantarum 299V (DSM9843) in an amount of ^{1X10 3 CFU / ml 1X10 8 CFU} / ml.

The culturing step may be carried out at a temperature range of 25 to 40 degrees for 12 to 96 hours, but it may be shortened to 24 hours or less.

The drying step includes a freeze-drying step, which may be carried out at -80 占 폚 or lower for 36 to 72 hours.

Next, the pharmaceutical composition, health functional food and cosmetic composition for preventing, ameliorating or treating infectious diseases caused by pathogenic skin disease causative bacteria of the present invention include a biodegradable elm extract as an active ingredient. As a result, the pharmaceutical composition of the present invention can treat infectious diseases induced by oral or parenteral administration for the treatment of infectious diseases caused by causative bacteria of pathogenic skin diseases of mammals other than humans.

The pharmaceutical compositions of the present invention may comprise a pharmaceutically acceptable carrier. Compositions comprising a pharmaceutically acceptable carrier may be in the form of tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterile aqueous solutions, nonaqueous solutions, suspensions, emulsions, lyophilized preparations, Oral or parenteral formulations selected from the group consisting of In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose or lactose, gelatin, . In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like may also be used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the non-aqueous solvent and the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.

The elm extract used for the biological conversion in the present invention may be one obtained by extracting at least one of the branches, shells, leaves and fruits of elm with water, a C1 to C2 lower alcohol or a mixture thereof as a solvent. The elm extract used in the biologically converted elm extract contained in the functional food may be extracted with water, ethanol or a mixture thereof as a solvent.

Example

1. Badge preparation step

① Preparation of elm extract

100 g of dried elm and 1 L of water are placed in a hot water bath and hydrothermal extraction is carried out. Extracts of elm were extracted from 500 ml of hot water extract obtained by hot water extraction using a paper filter to remove impurities.

② MRS broth powder of lactic acid bacteria was melted by adding 55 grams of MRS broth powder per 1 liter of elm extract to the elm extract.

2. Inoculation phase

Lactic acid Lactobacillus planta 299V (DSM9843) was inoculated to the MRS medium containing elm extract at a concentration of 1 × 10 ⁶ CFU / ml.

3. Culture step

The lactic acid bacteria inoculated to carry out the biotransformation of the elm extract were incubated at 37 ° C for 24 hours to obtain a biotransformed culture of the elm extract.

4. Removal steps

The biotransformed cultures were centrifuged at 7000 rpm for 30 minutes and filtered using a 0.45 ㎛ syringe filter to remove the lactic acid bacteria contained in the biotransformed cultures.

Experimental Example 1

The pH of the biotransformed elm extract after bioconversion using the elm extract and lactic acid bacterium obtained in the Examples was measured and the results are shown in Table 1 and FIG. From Table 1 and FIG. 2, the pH of the elm extract before the bioconversion was 6.15, and the pH of the control MRS medium and the biosensitive elm extract was 4.19, .

Experimental Example 2

The antibacterial effect of the cultured lactic acid bacteria, the elm extract obtained in the examples and the biologically transformed elm extract (liquid phase) against skin disease causative bacteria was confirmed as follows.

Here, Lactobacillus plantarum 299V was cultured in MRS medium and elm extract MRS medium. The pathogens used were confirmed to have antimicrobial inhibitory effects on three species of Propionibacterium acnes KCTC 3314, Streptococcus pyogenes KCCM 11873 and Staphylococcus aureus KACC 13236 . Streptococcus pyogenes KCCM 11873 was cultivated in a medium containing 5% Sheep blood in a TS (Tryptic-Soy) medium, in a medium of Staphylococcus aureus KACC 13236 was cultured in a TS (Tryptic-Soy) medium.

Specifically, the paper disc method was used to investigate the antibacterial activity. Lactobacillus cultures, elm extracts, and biotransformation elm extracts were spotted on 8 mm paper discs in 100 μl aliquots at 1 × 10 ⁶ CFU / ml, respectively. The medium on which the paper discs were germinated was cultured for 24 hours at 37 ° C to confirm whether or not growth inhibition rings were produced. The results are shown in Tables 2, 3 and 4

As can be seen from Table 2, FIG. 3 and FIG. 4, the antimicrobial activity was not detected in all the skin diseases caused by the elm extract, and the antimicrobial activity was confirmed in S. aureus in the Lactobacillus MRS culture. However, antimicrobial activity was also confirmed in P. acnes and S. pyogenes , which were not detected in the MRS culture broth or elm extract, in the biotransformed elm extract, and the antimicrobial activity of S. aureus was increased by 7%.

The simple elm extract showed no antimicrobial activity against all skin disease causative bacteria, but the biodegradation elm extract had antimicrobial activity against all skin disease causative bacteria. The results showed that the increase of antimicrobial activity of elm extract was higher than that of L. plantarum species It is possible to confirm that this is due to bioconversion.

From the above results, it was confirmed that the biologically-transformed elm extract of the present invention can be used as a functional food material or a cosmetic material as well as a novel pharmaceutical composition for prevention of infectious diseases caused by pathogenic skin disease bacteria and symptom improvement. In particular, the above-mentioned Experimental Example shows the antimicrobial activity of the skin-causing causative bacteria of the biologically-transformed elm extract using lactic acid bacterium. Among the causative strains exhibiting the antimicrobial activity of the biodegradable elm extract of the present invention, , It can be used as a composition for alleviating atopic pruritus, cosmetics for alleviating atopic pruritus such as lotion and skin, using the biologically converted elm extract of the present invention.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation, Various changes and modifications will be possible.

Claims (16)

Biologically Transformed Elm Tree Extracts with Antimicrobial Activity against Causing Skin Diseases.
The method according to claim 1,
Wherein the antimicrobial activity is imparted through the biosynthesis of the lactic acid bacteria of the elm extract.
The method according to claim 1,
Wherein the skin disease causative microorganism is at least one selected from the group consisting of purulent Streptococcus pyogenes , Propionibacterium acnes , and Streptococcus pyogenes .
3. The method of claim 2,
The lactic acid bacteria used for the conversion of the above- Wherein the extract is Lactobacillus plantaurum .
5. The method of claim 4,
Wherein the Lactobacillus plantaurm is L. plantarum 299V (DSM 9843).
A medium preparation step of preparing an elm extract-containing liquid medium by adding the elm extract to a lactic acid bacteria culture composition;
An inoculation step of inoculating the elm extract-containing liquid medium with the lactic acid bacteria; And
Culturing the lactic acid bacterium so as to carry out bioconversion of the elm extract in the inoculated state to obtain a bioconversion culture.
The method according to claim 6,
Further comprising a removing step of removing the lactic acid bacteria contained in the biotransformation culture.
The method according to claim 6,
The method further comprises a step of drying the bioconversion culture obtained in the culturing step or further comprising the step of removing the lactic acid bacteria contained in the bioconversion culture and drying the bioconversion culture in which the obtained lactic acid bacteria have been removed Characterized by comprising a biodegradable elm extract preparation method.
The method according to claim 6,
Wherein the lactic acid bacteria inoculated in said inoculation step is Lactobacillus plantaurum .
10. The method of claim 9,
Wherein said Lactobacillus plantaurm is L. plantarum 299V (DSM 9843) and said Lactobacillus plantaurm is inoculated at a content of 1 X 10 ³ CFU / ml to 1 X 10 ⁸ CFU / ml. .
The method according to claim 6,
Wherein the culturing step is carried out at a temperature range of 25 to 40 degrees for 12 to 96 hours.
A biologically active elm extract according to any one of claims 1 to 5 or a biologically active elm extract prepared by the method of any one of claims 6 to 11 as an active ingredient, A pharmaceutical composition for preventing, ameliorating or treating an induced infectious disease.
13. The method of claim 12,
Wherein the disease caused by the pathogenic skin disease bacteria is at least one of laryngitis, tonsillitis, scarlet fever, hunger, sepsis, acne, sacchoidosis, uveitis, conjunctivitis, keratitis, skin rash, chronic inflammation and pyogenic infections. A pharmaceutical composition for preventing, ameliorating or treating an infectious disease caused by a causative agent of a disease.
A method for producing a biologically active elm extract of any one of claims 1 to 5 or a biosensitive elm extract prepared by the method of any one of claims 6 to 11 as an active ingredient, Health functional foods with antimicrobial activity. A method for treating an infectious disease caused by pathogenic skin disease causing bacteria, comprising the step of administering the pharmaceutical composition of claim 12 to a mammal other than a human.
A method for producing a biologically active elm extract according to any one of claims 1 to 5 or a biologically transformed elm extract prepared by the method according to any one of claims 6 to 11 as an active ingredient, A cosmetic composition having antimicrobial activity against bacteria.

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