KR20170141972A - Composition for preventing, improving or treating obesity comprising fermented Geomgangsong as effective component - Google Patents

Abstract

The present invention relates to a composition for preventing, ameliorating, and treating obesity containing fermented Geomgangsong (specific Korean pine tree) as an active ingredient. More specifically, the fermented Geomgangsong, which is obtained by primarily fermenting Geomgangsong leaf or branch powder with alcohol and then secondarily fermenting the alcohol-fermented product with acetic acid, is capable of suppressing adipocyte differentiation. Accordingly, the fermented Geomgangsong of the present invention can be widely used in industries associated with obesity.

Description

Technical Field [0001] The present invention relates to a composition for prevention, improvement and treatment of obesity comprising a fermented product as an active ingredient,

The present invention relates to a composition for preventing, ameliorating and treating obesity, which comprises a fermented product of a goldsmith carrier as an active ingredient.

Obesity is a phenomenon caused by an imbalance in consumption and consumption of energy, which means that the body is over-accumulated. Although it is not clear yet why the cause is caused by various causes, it is generally recognized that it is caused by excessive calorie intake, endocrine disruption, lack of exercise, genetic factors, and the like. It is also commonly referred to as lifestyle-related diseases because it is closely related to lifestyle habits. The worldwide obesity population is steadily increasing. As the income level and eating habits are westernized, the number of obese people in Korea is increasing rapidly. According to the national health statistics, the prevalence of obesity over 19 years of age continuously increased from 26.0% in 1998 to 31.0% in 2008, which is 32.2% in the United States (over 20 years old, body mass index 30kg / Similar level. Obesity has become a serious social problem, and various measures and active researches are under way in Korea to prevent obesity, which is a major cause of adult diseases. As a method for healing obesity, there are improvement of lifestyle such as eating, drug administration, and surgery. Recently, as the market for health functional foods has increased, many attempts have been made to improve functional foods in parallel with dietary control. Has been enacted, and a variety of health functional materials and foods have been licensed by the Korea Food and Drug Administration. Although many efforts have been made to prevent, ameliorate, or treat obesity, obesity is still increasing.

On the other hand, pine trees and pine needles have been known to have antimicrobial, sterilizing and deodorizing functions from the past, and Kumkansong, which is also called "Chungyangmok", is known to have excellent deodorizing action due to its unique wood structure. As a related art including pine needle as an active ingredient, Korean Patent Laid-Open Publication No. 2014-0036053 discloses a technique for improving metabolic syndrome improving functional rice cakes containing pine needle juice as an active ingredient, and Korean Patent No. 1326271 Discloses a method for producing a fermented dermis extract having an anti-obesity effect and a fermented fermented extract thereof. Korean Patent Registration No. 0126380 discloses a composition for weight loss, No description has been made on a composition for preventing, ameliorating or treating obesity.

The present invention provides a composition for preventing, ameliorating and treating obesity comprising a fermented product of a goldsmith carrier as an active ingredient. The present invention provides a composition for prevention, improvement and treatment of obesity, The inventors of the present invention have completed the present invention.

In order to achieve the above object, the present invention provides a health functional food composition for preventing or ameliorating obesity, comprising a fermented product as an active ingredient.

The present invention also provides a pharmaceutical composition for preventing or treating obesity, which comprises a fermented product as a active ingredient.

The present invention relates to a composition for prevention, improvement and treatment of obesity, which comprises a fermented product of a goldfish sprout as an active ingredient. More specifically, the fermented product of a goldfish sprout is a fermented product of primary fermentation It is fermented with ferric acetic acid and has an effect of inhibiting accumulation of adipocytes. Therefore, the gold-smelting fermented product of the present invention can be widely used in an obesity-related industry.

FIG. 1 schematically shows a manufacturing process of a gold-smelting fermentation product of the present invention.
FIG. 2 is a graph showing the difference in the sugar content and the alcohol content of the fermented alcohol according to the addition amount of the poultry meal powder according to an embodiment of the present invention.
FIG. 3 is a graph showing the degree of sugar content and alcohol content of an alcoholic fermented product according to addition of sugar (A), apple concentrate (B) and brown rice (C) This is the result of confirming the difference.
FIG. 4 is a graph showing changes in sugar content and alcohol production of alcohol fermented according to the initial sugar content (12 to 16 Bricks) of the culture media of Kumkang River according to an embodiment of the present invention.
FIG. 5 is a graph showing changes in sugar content and titratable acidity of acetic acid fermented product according to addition of sugar (A), apple concentrate (B) and brown rice (C) titratable acidity.
FIG. 6 shows the results of confirming titratable acidity and alcohol content changes of the acetic acid fermented product according to acidity difference (1 to 3%) before acetic acid fermentation according to an embodiment of the present invention.
FIG. 7 shows the result of confirming the rate of inhibition of fat accumulation in the fermented product of the poultry meat according to an embodiment of the present invention.

The present invention relates to a health functional food composition for preventing or ameliorating obesity, comprising a fermented product of a goldsmith carrier as an active ingredient.

The fermented product of the present invention is preferably fermented by a fermented tea leaf or a powder of a branch, but is not limited thereto. The fermented product of the present invention is characterized by inhibiting accumulation of adipocytes. The fermented fish product of the present invention can be obtained by (1) culturing Saccharomyces cerevisiae cerevisiae , and (2) fermenting the fermented product obtained in (1) above to a fermented product of Acetobacter but it is not limited thereto. Any of the strains fermenting alcohol or fermenting acetic acid may be selected and used. Preferably, the fermentation liquid includes 5 to 30% by weight of the pneumoconiotic powder, and more preferably 15% by weight of the pneumoconiotic powder. However, the present invention is not limited thereto.

The culture medium for suspension culture may further contain sugar, an apple concentrate or a brown rice in addition to the pest control powder, preferably an apple concentrate, but the present invention is not limited thereto.

Preferably, the initial sugar content of the cultured poultry culture medium is 3 to 16 Bricks, more preferably 12 Bricks, but the present invention is not limited thereto. The initial acidity of the acetic acid fermentation is 1 to 3%, but is not limited thereto.

The alcohol fermentation and the acetic acid fermentation are preferably performed at 28 to 32 ° C. for 3 to 5 days, more preferably at 30 ° C. for 4 days for fermentation of alcohol and acetic acid.

The health functional food composition containing the fermented product of the present invention as an active ingredient for preventing or ameliorating obesity can be manufactured by any one selected from beverage, ring, tablet, capsule and powder, , And may be suitably prepared according to a conventional method.

Examples of foods to which the health functional food composition of the present invention can be added include meat products, sausages, breads, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, Beverage, tea, drink, alcoholic beverage, and vitamin complex, and includes all the health foods in the ordinary sense.

The health functional foods include various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, colorants and enhancers (cheese, chocolate etc.), pectic acid and its salts, alkynic acid and its salts, , pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. It may also contain flesh for the production of natural fruit juices and vegetable drinks. These components may be used independently or in combination.

The health functional food composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient. The natural carbohydrates are sugar saccharides such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, xylitol, sorbitol and erythritol. As the sweetening agent, natural sweetening agents such as tau Martin and stevia extract, synthetic sweetening agents such as saccharin and aspartic acid, and the like can be used.

The present invention also relates to a pharmaceutical composition for preventing or treating obesity, which comprises a fermented product of a goldsmith carrier as an active ingredient. In addition to the above-mentioned fermentation product for a goldsmith, it is preferable to further include a carrier, an excipient or a diluent, but is not limited thereto.

The pharmaceutical composition of the present invention may be various oral or parenteral formulations. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules, and the like, which may contain one or more excipients such as starch, calcium carbonate, sucrose or lactose lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like may also be used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of non-aqueous solvents and suspensions include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like. Examples of suppositories include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and it is preferable to select the intraperitoneal, rectal, rectal, intravenous, intramuscular, subcutaneous, intrauterine or intracerebral injection methods during parenteral administration, It is preferably used for external skin application.

The pharmaceutical composition according to the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level will depend on the type of disease, severity, , Sensitivity to the drug, time of administration, route of administration and rate of release, duration of treatment, factors including co-administered drugs, and other factors well known in the medical arts. The pharmaceutical composition of the present invention can be administered as an individual therapeutic agent or in combination with other therapeutic agents, and can be administered sequentially or simultaneously with conventional therapeutic agents, and can be administered singly or in multiple doses. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without side effects, which can be easily determined by those skilled in the art.

The dosage of the pharmaceutical composition of the present invention may be varied depending on the patient's body weight, age, sex, health condition, diet, administration time, administration method, excretion rate, and severity of the disease.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

[Materials and Methods]

1. Materials

The Kumkang shrimp by - product (Kumkwang powder) was purchased from Uljin Forestry Cooperative, and the apple concentrate was purchased from Elim Cai and used as raw material.

2. Use strain and medium composition

Alcohol fermentation strain Saccharomyces cerevisiae was subcultured in YPD plate medium and cultured in YPD liquid medium.

Acetic acid fermentation strains were obtained from Acetobacter sp.), and the composition of the culture medium is shown in Table 1 below.

Culture medium culture of acetic acid bacteria Composition ratio(%) Yeast extract 0.5 Glucose 0.5 glycerin 1.0 MgSO ₄ .7H ₂ O 0.02 ethanol 5.0 Acetic acid 1.0 water 91.98

3. Preparation of Kumhwang Thrapen by-product (Kumhwang powder)

The collected ginseng shrimp by - products (leaves and branches) were washed and immersed in water. After the water was removed well, it was pulverized by crushing, pulverized, dried and used for fermentation.

4. Alcohol fermentation conditions

1) Alcohol fermentation according to the content of Kumhang shim by-product

The apple concentrate (70 ° brix) was diluted in purified water and adjusted to a sugar degree of 12 ° brix. The fermentation broth containing 5, 10, 15 and 30% (w / v) Saccharomyces S. cerevisiae were inoculated and fermented at 30 DEG C for 96 hours.

2) Alcohol fermentation according to kinds of additives (sugar, apple concentrate and brown rice)

When alcohol fermentation, sugar, brown rice, and apple concentrate were added and the initial sugar content was adjusted to 3 ~ 12 Bricks and fermented for 96 hours. The sugar content and alcohol content were measured at 24 hour intervals.

3) Alcohol fermentation according to initial sugar content

The initial sugar content of the fermentation broth was adjusted to 12 ~ 16 ° brix and the sugar content and alcohol content were measured at 24 - hour intervals.

5. Acetic acid fermentation conditions

1) Acetic acid fermentation according to kinds of additives (sugar, apple concentrate and brown rice)

To the fermentation broth of alcohol fermented by adding sugar, apple concentrate and brown rice to 15% (w / v) shrimp by - product (Kumunggwang powder), acetic acid bacteria Acetobacter sp.) and incubated at 30 캜 for 180 hours at 180 rpm for acetic acid fermentation.

2) Optimum conditions of acetic acid fermentation of Kumhang shim by-product

In order to investigate the optimal acetic acid fermentation condition of Kumkang shrimp by - product (Kumkwang powder), the initial acidity of the end - of - fermentation solution was adjusted to 1 ~ 3% and the alcohol content was changed at 24 - Acetic acid content was measured.

Example  1. Establishment of alcohol fermentation condition according to the contents of Kumhwang's thinning by-product

5, 10, 15 and 30% (w / v) gangshang thinning by-products (Kum-gang Song powder) were added to the fermentation broth containing 12 Bricks of apple concentrate as an initial condition for alcohol fermentation of Kum- , Celebi as Saccharomyces My jiae access (Saccharomyces S. cerevisiae were inoculated and fermented at 30 DEG C for 96 hours.

As a result, the sugar content decreased and the alcohol content increased with time in all experimental groups. In particular, the alcohol content was highest when 15% (w / v) gangshang thinning by-product (Kumkwang powder) was added to the final product, and 30% (w / v) In one case, the alcohol fermentation did not occur well, and the alcohol content was found to be the lowest (Fig. 2). From these results, it can be concluded that the concentration of by - product of Kum - gum shrimp (Kum - shong powder) is too high and the fermentation does not progress well.

Example  2. Alcohol fermentation according to kinds of additives

In order to investigate the effect of additives on the alcohol fermentation of alcoholic fermentation, we used sugar (12 bricks) in 15% (w / v) , Apple concentrate (12 bricks) or brown rice (3 bricks) were added to perform alcohol fermentation.

As a result, it was confirmed that alcohol fermentation proceeded normally in all experimental groups, and alcohol content was highest when alcohol concentrate was added in the alcohol fermentation with addition of sugar, apple concentrate or brown rice (FIG. 3).

Example  3. Alcohol fermentation of Ganghwangsun thinning by-product (Kumunggwang powder) according to initial sugar content

In order to investigate the effect of initial sugar content on fermentation during alcohol fermentation, 15, 12, 14, and 16 brix apple concentrates were added to the shrimp by - product of 15% (w / v) And the fermentation was carried out after controlling the initial sugar content.

As shown in FIG. 4, alcohol fermentation proceeded normally in all groups, and the alcohol content was increased in proportion to the initial sugar content, but the difference was not significant.

Therefore, considering fermentation efficiency and economical aspect, it was judged that fermentation with 12 brix apple concentrate is most suitable.

Example  4. Acetic acid fermentation of Kumkang shim by-product (Kumkwang powder) according to the kind of additives

In order to investigate the effect of supplementary ingredients on the acetic acid fermentation during the acetic acid fermentation using alcohol obtained by alcohol fermentation of 15% (w / v) shrimp by - product (Kumunggwang powder) The concentrate or brown rice was added to conduct alcohol fermentation.

As a result, it was confirmed that the acetic acid fermentation proceeded normally in all the experimental groups. When the sugar, the apple concentrate or the brown rice was added, the acetic acid fermentation was performed after the alcohol fermentation, when the apple concentrate was added, (Fig. 5).

Example  5. Establishment of Acetic Acid Fermentation Condition of Kumkang Stream Thumbnail By-product (Kumkang Song Powder) according to Initial Acidity

Initial acidity was adjusted to 1, 2, and 3% (v / v) to investigate the effect of initial acidity on the acetic acid fermentation of Kumkangsong thinning by - product (Kumkwang powder).

As a result, the acetic acid fermentation proceeded smoothly at 1% (v / v) and the fermentation rate was slower than 1% (v / v) at 2 and 3% (v / v) 6).

Example  6. Ganghwangsun thinning by-product (Kumkwang powder) Fermented  Produce

On the basis of the results of Examples 1 to 5, 15% (w / v) Kumung Shipping Powder and 12 Bricks of apple concentrate were added to perform alcohol fermentation for 4 days, initial acidity was adjusted to 1% The fermentation was carried out to confirm that the acidity was the most effective.

Example  7. Including Ganghwanggang thinning by-product (Kumkangshan powder) Fermented  Evaluation of anti-obesity efficacy

The cytotoxicity and inhibitory rate of adipocyte differentiation of the fermented product obtained in Example 6 were found to be about 80% or less when treated at a concentration of 200 μg / ml, and the final fermentation product (acetic acid fermentation) 100, 200 and 400 / / ml, inhibition of adipocyte differentiation was observed. Especially, when treated at 400 / / ml, the inhibition rate of adipocyte differentiation was about 23% (Fig. 7).

Claims (10)

A health functional food composition for preventing or ameliorating obesity, which comprises a fermented milk product as an active ingredient. The health functional food composition for preventing or improving obesity according to claim 1, wherein the fermented product is fermented with a powder or a powder of a garment. The health functional food composition for preventing or ameliorating obesity according to claim 1, wherein the fermented milk fermented product inhibits fat cell accumulation. The fermented product according to claim 1, wherein the fermented product is (1) Saccharomyces cerevisiae , and (2) fermenting the fermented product obtained in (1) above to a fermented product of Acetobacter The present invention relates to a health functional food composition for preventing or ameliorating obesity. The health functional food composition for preventing or improving obesity according to claim 4, wherein the culture fluid for culture media comprises 5 to 30% by weight of pneumoconiosis powder. 5. The health functional food composition according to claim 4, wherein the culture medium for slimming further comprises at least one selected from the group consisting of sugar, apple concentrate and brown rice in addition to a pearl powder. The health functional food composition for preventing or improving obesity according to claim 4, wherein the culture fluid for slimming has an initial sugar content of 3 to 16 Bricks. The health functional food composition according to claim 4, wherein the initial acidity of the acetic acid fermentation is 1 to 3%. The health functional food composition according to claim 4, wherein the alcohol fermentation and the acetic acid fermentation are carried out at 28 to 32 ° C for 3 to 5 days. A pharmaceutical composition for the prevention or treatment of obesity, comprising fermented milk powder as an active ingredient.

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