KR20170136337A - Cosmetic Composition containing Sea cucumber processing by-products containing Chondroitin sulfate and Hyaluronic acid - Google Patents

Abstract

The purpose of the present invention is to provide a cosmetic composition containing sea cucumber processed by-products containing chondroitin sulfate and hyaluronic acid. More specifically, provided is a cosmetic composition for wrinkle alleviating, whitening, moisturizing and skin elasticity promoting purposes containing sea cucumber processed by-products containing chondroitin sulfate and hyaluronic acid.

Description

(Cosmetic Composition containing Sea cucumber processing by-products containing Chondroitin sulfate and Hyaluronic acid) containing chondroitin sulphate,

The present invention relates to a cosmetic composition containing a sea cucumber sulfate-containing extract of sea cucumber containing a chondroitin sulfate and a cosmetic composition containing hyaluronic acid. More particularly, the present invention relates to a cosmetic composition containing chondroitin sulphate-containing sea cucumber extract and hyaluronic acid for improving wrinkles, Or elasticity-enhancing cosmetic composition.

One of the greatest areas of interest in cosmetics is wrinkling, pigmentation, loss of elasticity, dryness and hair loss associated with aging, or lack of gloss in the hair. The skin is subject to various changes through aging. First, the thickness of the epidermis, dermis, and subcutaneous tissues of the skin becomes thinner and the ECM (Extracellular matrix) component that gives skin elasticity changes. Among them, the collagen which accounts for 70 ~ 80% Collagen, elastin, proteoglycans, glucosaminoglycan, laminin, lipid, and the like, which are closely related to wrinkle formation, Fibronectin, etc. are oxidized and lose their function, causing the skin to lose its elasticity and wrinkles becoming excessively formed and changing to senile skin. The connective tissue of the ECM (Extracellular matrix) is composed of glue fibers (collagen fiber, reticular fiber, elastic fiber, elastic fiber), of which 70% Collagen is mostly formed in fibroblasts of the skin. As you get older, the collagen content in the connective tissue of the skin decreases, which is due to degradation of collagen synthesis and promotion of degradation. Therefore, degradation of collagen biosynthesis and promotion of collagen degradation are the major causes of wrinkles in the skin. Collagen biosynthesis is regulated by many factors involved in transcription and post-translational levels, and collagen degradation is mediated by the expression of matrix metalloproteases (MMP), such as collagenase, which degrades collagen by ultraviolet light. Promotes collagen degradation and reduces collagen content. In addition, as the collagen's deformation accelerates due to the external environment, the skin becomes wrinkled and deepened. As a result, skin aging is caused by non-homogenization of cells, loss of elastin, destruction of collagen, reduction and delay of collagen synthesis, and the like. Therefore, the skin aging phenomenon occurs in the epidermis of the skin but can be seen as a phenomenon that occurs in the dermis.

Before the aging process, the skin cells are activated physiologically. Therefore, in the fibroblast of the dermis, synthesis and metabolism of collagen which is important for suppressing elasticity and wrinkling of the skin are active, GAGS (Glucosaminoglycans), a glycoprotein containing hyaluronic acid, essential for moisture retention, is also active, giving the skin elasticity, softness, flexibility and moisturizing properties. In the epidermis, the proliferation of basal layer cells is active and the production of binding proteins such as laminin, integrin, and desmosomes, which enhance the binding between skin cells, is balanced, To maintain healthy skin. However, contemporary human skin is exposed to various harmful environments and stresses, and various kinds of damage are being experienced. As the age of the skin lowers the physiological activity of the skin and the recovery speed of the damaged skin is slowed down, the skin is deteriorated in elasticity, There are various skin aging phenomena such as calmness, black spots, and dullness. Therefore, the biggest goal of cosmetics is to improve the symptoms of aging, and a variety of cosmetics are being developed for this purpose.

In general, skin aging phenomena such as skin elasticity reduction, skin wrinkles and pigmentation are caused by collagen breakdown, reduction of collagen synthesis, disappearance of elastin, and melanin pigmentation due to lowering of skin physiology. It is a phenomenon. In recent years, many skin physiological studies have been conducted to prevent skin aging. Actually, ultraviolet ray blocking agents, antioxidants, cell activation promoters and the like have been used to prevent skin wrinkles in cosmetics and methods for improving skin wrinkles due to collagen synthesis have been proposed . On the other hand, based on the advances in biochemistry and molecular biology, a small amount of signal substances such as growth factors existing in the living body have been found, and the aging theory of living bodies based on them has been reestablished. It is also found that this signaling substance decreases in vivo with increasing age, which is closely related to our aging.

It effectively protects the skin from harmful factors such as sunlight in the sunlight. However, as age increases, the skin texture gradually changes and the ability to defend against sunlight is lowered. As a result, wrinkles, pigmentation, sagging skin, loss of skin elasticity, Roughness, skin dryness, hair loss, and hair gloss reduction. The result of accumulation of such effects by daylight is called photoaging.

Various cosmetic compositions for solving the problem of aging of the skin have been studied, and the wrinkle-reducing effect of the skin has shown some visible results. There have been various reports on retinoids widely used for improving wrinkles and wrinkles and pigmentation of the skin, among which retinol has been reported variously for removing wrinkles on retinol. Various retinol-containing cosmetics have been used for the treatment of skin wrinkles and skin Deflection, and elasticity. U.S. Patent Nos. 4,603,146 and 4,877,805 disclose examples of improving wrinkles using retinol (vitamin A), which is effective in collagen synthesis and inhibition of collagen degradation by inhibiting collagen smoothness activity.

It has also been reported that a cosmetic composition containing L-ascorbic acid (vitamin C), which is essential for the collagen synthesis process, is effective for skin wrinkle improvement, ultraviolet light blocking, pigmentation such as spots / freckles / black spots (US Pat. No. 4,938,969 / USP4, 772,591 / EP0533667).

In addition, a cosmetic composition containing an α-hydroxy acid (AHA), which is effective in removing exfoliation, is also widely used for improving the skin by promoting cell activity along with exfoliation.

The inventors of the present invention have found that a cosmetic composition comprising chondroitin sulfate-containing sea cucumber by-product extract and hyaluronic acid as an active ingredient has a wrinkle-improving effect, a whitening effect, a moisturizing effect and a skin elasticity-enhancing effect.

It is an object of the present invention to provide a cosmetic composition containing a sea cucumber sulfate-containing extract of sea cucumber containing a chondroitin sulfate and a cosmetic composition containing hyaluronic acid. More particularly, the present invention relates to a sea cucumber processing by-product extract containing chondroitin sulfate, And a cosmetic composition for moisturizing or improving skin elasticity.

The present invention relates to a cosmetic composition comprising a sea cucumber processing by-product extract containing chondroitin sulfate and hyaluronic acid, wherein the cosmetic composition comprises 0.5 to 20% by weight of a sea cucumber processing by-product extract and 0.1 to 2.0% by weight of hyaluronic acid Wherein the weight ratio of the sea cucumber processing by-product extract containing the chondroitin sulfate to the hyaluronic acid is from 5: 1 to 30: 1.

The cosmetic composition is used for skin wrinkle improvement, whitening, moisturizing, skin elasticity enhancement.

Preferably, the sea cucumber processing by-product extract is a hot-water extract of sea cucumber which is prepared by adding sea cucumber into water and heated at a temperature of 60 ° C to 100 ° C for 1 to 6 hours.

The present invention relates to a cosmetic composition containing a chondroitin sulfate-containing by-product extract containing a chondroitin sulfate and a cosmetic composition containing a hyaluronic acid, thereby exhibiting superior skin wrinkle-improving effects, whitening Effect, moisturizing effect and skin elasticity enhancement effect can be obtained.

In addition, it is useful for protecting the environment by utilizing the abandoned sea cucumber processing by-products (mostly built-in) in the course of processing the conventional sea cucumber.

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention. The contents of all publications referred to herein are incorporated herein by reference. The term " drug " is used in reference to a reference amount, level, value, number, frequency, percentage, dimension, size, amount, weight, or length of 30, 25, 20, 25, 10, 9, 8, 7, 6, Level, value, number, frequency, percentage, dimension, size, quantity, weight or length of a sample,

Throughout this specification, the words " comprising " and " comprising ", unless the context clearly requires otherwise, include the steps or components, or groups of steps or elements, And that they are not excluded.

As used herein, the term " by-product of sea cucumber processing "or" mature water "means a by-product of the process of processing sea cucumber and includes various parts of sea cucumbers including viscera. In addition, the "sea cucumber processing by-product extract" of the present invention includes all of the extracts obtained from the sea cucumber processing by-product or from the water of cooking water. Specifically, the "sea cucumber processing by-product extract" of the present invention means a hydrothermal (water) . The sea cucumber processing by-product extract may be prepared using a method of preparing a sea cucumber processing by-product extract (dipping method), preferably sea cucumber is added to water, and then the mixture is heated at a temperature of 60 ° C to 100 ° C for 1 to 6 hours Can be prepared by heating.

The hyaluronic acid of the present invention was first obtained from a vitreous membrane of cattle by Meyer et al. In 1934 and was named O - β -D-glucuronosyl (1 → 3) -N -acetyl- and is a representative type of glycosaminoglycan having a structure in which only 2 sugars of ? -D-glucosaminyl (1? 4) units repeat. Hyaluronic acid is widely and distributed in the connective tissues of mammals, and is found in many kinds of chicken eggs, skin, skin, aorta and joint fluid. Other microorganisms such as streptococci are also reported to be produced. It is a complex polysaccharide composed of amino acid and uronic acid, and is a high molecular compound composed of N-acetylglucosamine and glucuronic acid. It is present in the vitreous body of the eye and the umbilical cord, and it plays a role to prevent infiltration of bacteria or penetration of poison. And chondroitin sulphate are known as major mucopolysaccharides. The ocular vitreous fluid, umbilical, dermal surface, such as connective tissue, grouse six kinds, including any kind of type A, insoluble Streptococcus type C in addition to those found in tumors, including (Streptococcus) some kind of bacteria is a capsular component. In animal tissues, such as bovine cartilage, large chondroitin sulfate proteoglycans are combined with aggrecan or binding proteins to form large molecular assemblies. The length of the sugar chain has a very long molecular weight of 10 ⁵ to 10 ⁶ , but it varies depending on the material, and even the same material is never uniform. It has a property of binding with a large amount of water to form a gel, which is associated with functions in the body such as joint lubrication and skin elasticity. In addition, if the connective tissue is damaged, the synthesis of hyaluronic acid is temporarily activated for rapid recovery, and it is considered that it forms part of cell activity in tissue reconstruction. CD44 was found as a hyaluronic acid binding protein on the cell surface. Enzymes that hydrolyze or cleave hyaluronic acid glucosamides or glucuronide bonds from animal tissues such as testis, skin, and liver, and snake venom, leech, bacteria, and actinomycetes, respectively, have been found. It is similar to the pectin of plants and is hydrolyzed by hyaluronidase.

Currently, the raw material for extraction of hyaluronic acid used in industrial production is a chicken farm, but recently it has become possible to mass-produce it by fermentation production of microorganisms (streptococci). In the preparation of hyaluronic acid in chicken broth, various proteins, other mucopolysaccharides, nucleic acids and the like are purified through enzymatic treatment and proteolytic cleavage and mucopolysaccharide separation by cetylpyridinium chloride method. On the other hand, Streptococcus (St. Zooepidemicus) by fermentation production is incubated about 48 hours in a culture medium a yeast extract and peptone to glucose as a carbon source to nitrogen source at generates a hyaluronic acid. After the cultivation is completed, the cells and the impurities are removed by filtration, and high-purity hyaluronic acid (protein content: 0.1% or less) is obtained by a simple operation such as alcohol precipitation. The hyaluronic acid used in the present invention has a molecular weight of 10 ³ to 10 ⁶ D (Molecular weight) obtained from microbial fermentation production.

The safety of purified hyaluronic acid is very safe because it shows negative in LD ₅₀ , subacute toxicity, mutagenicity test, eye mucosal irritation test, skin first stimulation test, cumulative skin irritation test and human patch test. The pharmacological actions of hyaluronic acid have been reported to inhibit migration of lymphocytes and macrophages, to participate in adhesion, aggregation and detachment of various cells, and to cure lacerations and wounds.

The hyaluronic acid of the present invention is widely used in cosmetics and medicines and has a high hygroscopicity and a unique feeling of use under almost the same conditions under low humidity and high humidity conditions for use in cosmetics and therefore can be used as a cream, a milky lotion, a lotion, a serum, Foundation cosmetics, make-ups such as oil-based lipsticks and foundations, and functional hair products.

Hyaluronic acid is being developed as a pharmaceutical product both domestically and abroad. In orthopedics, it is used as deformed arthropathy. In dermatology, it is used as laceration, mud, wounds, eye injections for cataracts in ophthalmology, and non-pyogenic arthritis drugs for racing horses in veterinary medicine. The application of hyaluronic acid, which can be mass-produced, will become more widespread in the future.

The cosmetic composition of the present invention may comprise a sea cucumber processing by-product extract containing from 0.5 to 20% by weight of chondroitin sulfate and 0.1 to 2.0% by weight of hyaluronic acid based on the total weight of the cosmetic. In addition, the weight ratio of the sea cucumber processing by-product extract containing chondroitin sulfate to hyaluronic acid may be from 5: 1 to 30: 1, and preferably from 5: 1 to 10: 1.

According to a specific example of the present invention, the cosmetic composition of the present invention containing chondroitin sulfate-containing sea cucumber processing by-product extract and hyaluronic acid as an active ingredient is effective for preventing skin aging such as skin wrinkle improving effect, whitening effect, moisturizing and skin elasticity .

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited by these Examples and Experimental Examples. For the cosmetic composition of the present invention, a cosmetic composition containing a sea cucumber processing by-product extract containing chondroitin sulfate and a hyaluronic acid was prepared as in the following examples.

Example  1. Method of manufacturing by-product extract of sea cucumber

The sea cucumber was placed in water and heated at a temperature of about 80 ° C for about 3 hours to prepare a water-in-water recipe for sea cucumber. 100 g of the water-processing by-product of the sea cucumber which has been thoroughly washed with purified water is finely ground with a blender and then subjected to ultrasonic disassembly (1 min on, 30 seconds off, 22.5 kHz) 10 times. Thereafter, the mixture was stirred at 60 DEG C and 300 rpm for 6 hours, and subjected to ultrasonic degradation under the same conditions. The supernatant was separated by centrifugation at 10000 rpm for 10 minutes, and centrifuged at 10000 rpm for 5 minutes. The supernatant thus obtained was filtered with a 6-μm filter to prepare an extract of by-products of sea cucumber of the present invention.

Example  2. Chondroitin sulfate  Containing sea cucumber processing by-product extract and hyaluronic acid Cosmetic  Produce

(Comparative Example 1) containing only a sea cucumber processing by-product extract containing chondroitin sulfate and a composition containing a hyaluronic acid (Example) and a sea cucumber processing by-product extract containing chondroitin sulfate (Comparative Example 1) and a composition containing only hyaluronic acid One composition (Comparative Example 2) was prepared.

Unit: wt% ingredient Example Comparative Example 1 Comparative Example 2 By-product extract of sea cucumber 5.0 5.0 - Hyaluronic acid 1.0 - 1.0 A Arachidyl alcohol 3.0 3.0 3.0 Glyceryl stearate 1.5 1.5 1.5 Squalane 5.0 5.0 5.0 Hydroxypolydecin 2.0 2.0 2.0 Trioctanoin 5.0 5.0 5.0 Polysorbate 80 1.0 1.0 1.0 Sorbitan stearate 0.5 0.5 0.5 Cyclopentasiloxane 3.0 3.0 3.0 Tocopheryl acetate 0.2 0.2 0.2 BHT 0.05 0.05 0.05 B Purified water Balance Balance Balance Concentrated glycerin 4.0 4.0 4.0 1,3-butylene glycol 2.0 2.0 2.0 Dimethicone / Vinyl Dimethicone Crosspolymer 0.4 0.4 0.4 EDTA-2Na 0.05 0.05 0.05 Incense, preservative Suitable amount Suitable amount Suitable amount system 100 100 100

Experimental Example  1. Skin wrinkle improvement effect

1. Mechanical evaluation

In order to measure the skin wrinkle improving effect (mechanical evaluation) of the cosmetic composition prepared according to the present invention, 30 women (mean age 48.5 years) older than 30 years were divided into three groups A, B and C The cosmetic compositions prepared according to the examples of Table 1 according to the present invention were applied to the A group, the B group, and the C group, respectively.

A replica of skin wrinkles was applied using a transparent silicone solution while applying to the subjects of groups A, B, and C for 2 weeks (2 times / day, 0.2g / times) And skin wrinkle change was measured with a skin wrinkle measuring device (SKIN VISIOMETER SV400, C + K Electronics GmbH, Germany). The replica image was three-dimensionally analyzed with a CCD camera, and the average roughness roughness (R _z ) of the following formula 1, which is a value obtained by dividing the sum of the roughness of each wrinkle (R _m : m is an integer of 1 or more) The improvement effect was analyzed. The test results are shown in Table 2 below.

Skin wrinkle improvement effect (6 weeks) Average roughness roughness (R _{z ,} μm) Example Comparative Example 1 Comparative Example 2 T0 T6 ΔR _z T0 T6 ΔR _z T0 T6 ΔR _z Subject 1 120 59 -61 131 92 -39 109 66 -43 Subject 2 137 64 -73 121 45 -76 129 80 -49 Subject 3 128 22 -106 129 74 -55 132 103 -29 Subject 4 119 40 -79 118 69 -49 118 85 -33 Subject 5 127 28 -99 120 58 -62 106 78 -28 Subject 6 140 21 -119 124 48 -76 135 83 -52 Subject 7 128 44 -84 119 40 -79 129 80 -49 Subject 8 145 38 -107 129 93 -36 118 63 -55 Subject 9 121 20 -101 115 35 -80 122 77 -45 Subject 10 129 47 -82 128 44 -84 125 81 -44 Average -91.1 -63.6 -42.7

As can be seen in Table 2, after 6 weeks, the example according to the present invention had an average wrinkle roughness (R _z ) of 91.1 μm (p <0.01), 63.6 μm (p <0.05) Comparative Example 2 was 42.7 탆 (p > 0.1), and the sea cucumber processing by-product extract containing chondroitin sulfate according to the present invention and the cosmetic containing hyaluronic acid were compared with a sea cucumber processing by-product extract containing chondroitin sulfate only About 43.2% w / w of Example 1, 113.3% w / w more improved than Comparative Example 2 containing only hyaluronic acid. Therefore, it can be seen that the cosmetic composition containing the chondroitin sulfate-containing sea cucumber extract and the hyaluronic acid-containing extract of sea cucumber containing the chondroitin sulfate according to the present invention has remarkably improved the wrinkle-reducing effect more effectively than the sea cucumber processing by-product extract or the cosmetic composition containing only hyaluronic acid alone.

2. Visual Evaluation

In order to measure the skin wrinkle improvement effect (visual evaluation) of the cosmetic composition prepared by the example containing the extract of sea cucumbers containing chondroitin sulfate according to the present invention and the hyaluronic acid-containing composition, 30 female Age 48.5 years) were divided into three groups, A, B, and C, respectively, and the cosmetic compositions prepared according to the examples of Table 1 according to the present invention were divided into Group A, Example B, Comparative Example 1, and C Comparative Example 2 was applied to each group.

The subjects were divided into 6 groups according to the subjective evaluation of the subjects after the application (2 times / day) centered around the eyes with wrinkles in the groups A, B and C for 6 weeks. (? W) was measured. The results are shown in Tables 3 and 4 below.

<Evaluation criteria for skin wrinkle improvement>

-3: Extremely worse -2: worse -1: slightly worse

0: No change 1: Slight improvement 2: Improvement 3: Highly improved

Clinical effect of skin wrinkle improvement (objective evaluation of expert) Skin wrinkle improvement (ΔW) Example Comparative Example 1 Comparative Example 2 T0 T6 T0 T6 T0 T6 Subject 1 0 3 0 One 0 One Subject 2 0 3 0 2 0 2 Subject 3 0 3 0 2 0 0 Subject 4 0 2 0 2 0 2 Subject 5 0 3 0 One 0 2 Subject 6 0 2 0 2 0 One Subject 7 0 3 0 2 0 2 Subject 8 0 3 0 2 0 0 Subject 9 0 3 0 3 0 One Subject 10 0 2 0 2 0 3 DELTA W (T6-T0) 2.7 1.9 1.4

Clinical effect of skin wrinkle improvement (subject's subjective evaluation) Skin wrinkle improvement (ΔW) Example Comparative Example 1 Comparative Example 2 T0 T6 T0 T6 T0 T6 Subject 1 0 3 0 2 0 2 Subject 2 0 3 0 2 0 2 Subject 3 0 3 0 One 0 One Subject 4 0 2 0 One 0 2 Subject 5 0 3 0 2 0 2 Subject 6 0 3 0 One 0 One Subject 7 0 3 0 3 0 2 Subject 8 0 3 0 3 0 2 Subject 9 0 3 0 3 0 One Subject 10 0 2 0 2 0 One DELTA W (T6-T0) 2.8 2.0 1.6

As can be seen from Tables 3 and 4, in the example of the cosmetic composition containing the sea cucumber processing by-product extract and the hyaluronic acid containing chondroitin sulfate according to the present invention, the objective evaluation of the expert and the subjective evaluation of the subject were 2.7 , 2.8, and the subjective evaluation of the subject increased by 40.0% from the comparative example 1 and by 75.0% from the comparative example 2, respectively, in the objective evaluation of the skilled person by 42.1% and 92.9% Of chondroitin sulfate containing chondroitin sulphate and a cosmetic composition containing hyaluronic acid are more effective in improving wrinkles than cosmetic compositions containing only sea cucumber by-product extract or hyaluronic acid.

Experimental Example  2. Skin whitening effect ( From the Melano site  Inhibitory effect on melanin production)

The melanocyte was purchased from a mouse-derived B-16 melanoma (ATCC CRL 6323) cell line. Melanoma cell line is inoculated into DMEM medium containing 4.5 g / L glucose, 10% serum and 1% antibiotic and cultured in a 50 ml T-flask at 37 ° C. After culturing under 5% CO ₂ for 24 hours, the cells were treated with 0.05% trypsin containing 0.02% EDTA and inoculated into a 50 ml T-flask and cultured for 48 hours. At this time, the number of cells is 5.76 × 10 ⁶ cells / flask. The cultured melanoma cells were diluted in DMEM medium at 1, 2, 5, 10, 20, 50, and 100 μg / ml, respectively, and the extracts of the sea cucumber processing by-products containing the chondroitin sulfate of the present invention and hyaluronic acid were treated at 37 &Lt; / RTI &gt; for 5 days. After the incubation, the culture medium is removed, and the cells are separated by treating with 1 ml of saline-phosphate buffer solution (PBS) containing 0.02% EDTA and 0.05% trypsin, followed by centrifugation for 5 minutes to collect only the cells. 5% trichloroacetate (TCA) is treated and stirred, and the precipitated melanin is centrifuged and washed with saline-phosphate buffer solution. The precipitated melanin is treated by dissolving 1 N NaOH and the absorbance is measured at 475 nm. The melanin concentration was determined from a standard concentration curve of synthetic melanin (Sigma). The experimental results are shown in Table 5.

Melanin synthesis inhibitory effect in melanocyte density
(占 퐂 / ml) Melanin production inhibition rate (%) By-product extract of sea cucumber + hyaluronic acid By-product extract of sea cucumber Hyaluronic acid No treatment - - - One 10.0 8.6 0.9 2 21.3 18.5 2.9 5 32.2 28.9 8.3 10 35.5 33.9 9.5 20 47.8 37.5 9.9 50 50.1 38.9 12.4 100 68.9 49.5 13.5

As can be seen from Table 5, when the sea cucumber processing by-product extract containing chondroitin sulfate and hyaluronic acid were treated at the same concentration, the sea cucumber processing by-product containing the chondroitin sulfate of Comparative Example 1 The effect of suppressing melanin synthesis was higher than that of the hyaluronic acid alone treated with the extract of Example 2. When the extract of sea cucumber containing the chondroitin sulfate of the present invention and the hyaluronic acid were used together, the extract of sea cucumber and the extract of hyaluronic acid were used alone The melanin production of melanocytes was more effectively inhibited and the skin whitening effect was shown.

Experimental Example  3. Skin elasticity improvement effect

Comparative Example 1 containing cosmetic composition containing chondroitin sulphate of the present invention and cosmetic composition containing hyaluronic acid and sea cucumber processing by-product extract, skin elasticity improvement for cosmetic composition containing only hyaluronic acid Comparative Example 2 The effect was measured.

30 women (mean age 48.5 years) 30 years old or older under the conditions of temperature 24-26 DEG C and humidity 45% were divided into three groups A, B and C, respectively, and the cosmetic compositions The composition was applied to the A group, to the group B of the comparative example 1, to the group C of the comparative example 2, and to the group C of the skin of the comparative example 2 with a skin elasticity tester (Cutometer SEM 575, C + K Electronic Co., Germany). The test results are shown in the following Table 6 as ΔR (R (before use) -R8 (after use)) value of Cutometer SEM 575. The R value represents the property of viscoelasticity of the skin.

Skin elasticity (after 6 weeks) Skin viscoelasticity improvement degree (? R) Example Comparative Example 1 Comparative Example 2 Subject 1 0.49 0.40 0.42 Subject 2 0.44 0.39 0.50 Subject 3 0.43 0.38 0.32 Subject 4 0.59 0.31 0.51 Subject 5 0.37 0.35 0.33 Subject 6 0.51 0.29 0.34 Subject 7 0.52 0.21 0.39 Subject 8 0.53 0.33 0.32 Subject 9 0.41 0.30 0.30 Subject 10 0.67 0.30 0.40 △ R 0.50 0.33 0.38

As can be seen from the results in Table 6, the cosmetic composition containing chondroitin sulphate-containing sea cucumber by-product extract and hyaluronic acid showed 51.5% skin elasticity and only hyaluronic acid in comparison with Comparative Example 1 containing only sea cucumber- Compared to Comparative Example 2, it was 31.6% higher than that of the cosmetic composition containing the sea cucumber processing by-product extract containing chondroitin sulfate and the cosmetic containing only hyaluronic acid.

Experimental Example  4. Moisturizing effect

The cosmetic composition containing the chondroitin sulphate-containing sea cucumber extract of the present invention, the cosmetic ingredient containing the hyaluronic acid, and the cosmetic ingredient containing only the sea cucumber processing by-product extract Comparative Example 1, the skin moisturizing effect of the cosmetic composition containing only hyaluronic acid Were measured. Measurements were made after stabilizing the skin at 24-26 ° C, 45% relative humidity and 20-30 minutes in the absence of air flow. The cosmetic composition prepared according to the example of the present invention divided into A, B, and C groups of 30 women (mean age 48.5 years) older than 30 years were classified into Group A, the cosmetic composition prepared according to Comparative Example 1 In the group B, the cosmetic composition prepared in Comparative Example 2 was applied to the C group for 6 weeks (twice / day) around the eye area and then transdermalized using TEWAMETER TM 210 (C + K electronic GmbH, Germany) (TEWL value change ΔTEWL) with time, and the change in the electrical conductivity according to the moisture content of the skin using CORNEOMETER CM 820 PC (C + K electronic GmbH, Germany) The moisture content of the skin was quantified to measure the moisturizing effect. The test results are shown in Table 7 below.

Skin moisturizing effect Test Products △ TEWL Corneometer Value Example 5.9 128 Comparative Example 1 3.8 101 Comparative Example 2 4.3 112

As a result of the test, it can be seen that the ΔTEWL value of the example is 5.9 g / hm ² , which is very effectively improved after 6 weeks of transdermal water loss compared to Comparative Example 1 and Comparative Example 2.

Also, the corneometer value obtained by measuring the moisture content of the skin is 26.7% higher than that of Comparative Example 1 and 14.3% higher than that of Comparative Example 2, indicating that the skin is very moist.

Experimental Example  5. Stability test of formulation

The stability test of the formulation of the sea cucumber processing by-product extract containing chondroitin sulfate of the present invention and the cosmetic containing hyaluronic acid was carried out. In order to test the stability of the cosmetic, the examples of Table 1, Comparative Example 1, and Comparative Example 2 were placed in a thermostatic chamber kept at 4 ° C and 45 ° C in a transparent glass container for 4 weeks, The degree of discoloration and discoloration (specific stiffness) was measured comparatively. The results are shown in Table 8 below. At this time, the product separation, discoloration, and degree of detachment were classified into the following six grades and evaluated.

Product Discoloration Evaluation Criteria:

0: No change 1: Very little separation (discoloration, change)

2: little separation (discoloration, discoloration) 3: slight separation (discoloration, discoloration)

4: Severely separated (discolored, detached) 5: Severely separated (discolored, detached)

Degree of separation Temperature detach discoloration Takeover Example compare
Example 1 compare
Example 2 Example compare
Example 1 compare
Example 2 Example compare
Example 1 compare
Example 2 45 ° C 0 0 0 0 0 0 0 0 0 4 ℃ 0 0 0 0 0 0 0 0 0

As can be seen from the above Table 8, the sea cucumber processing by-product extract containing the chondroitin sulfate of the present invention, the example containing the hyaluronic acid and the comparative example 1 containing only the sea cucumber processing by-product extract and the comparative example containing only the hyaluronic acid Are all stable and no separation, discoloration, and exfoliation phenomenon.

Experimental Example  6. Safety Test of Formulation

The skin safety test was conducted on the cosmetic composition containing the chondroitin sulfate-containing sea cucumber processing by-product extract of the present invention and hyaluronic acid.

20 patients (mean age 26.7 years, age range 18 to 30 years old) were formulated according to Examples and Comparative Examples of the present invention in accordance with Table 1, and then subjected to skin patch test (Hayashi's Test Chamber) ). Patients with psoriasis, eczema, other skin lesions, pregnant, lactating or contraceptive, and antihistamines were excluded from the study. The test site was wiped with 70% ethanol and dried. After dropping 15 μg of each of the Example and Comparative Example 1 and Comparative Example 2 into the chamber, the sample was placed on the top of the test site and fixed. After applying the patch for 24 hours and removing the patch, mark the test area with a marking pen and observe the test area after 24 hours and 48 hours, respectively. The determination was made at 24 hours and 48 hours after the removal of the patch, and the skin reaction was judged according to the rules of the International Contact Dermatitis Research Group (ICDRG) of Table 9 below. The test results are shown in Table 10 below as skin stability.

sign Criteria evaluation Average score ±
+
++
+++
- Doubtful or slight reaction and erythema
Erythema + Induration
Erythema + Induration + Vesicle
Erythema + Induration + Bullae
Negative Small stimulus
Light stimulus
Moderate irritation
River stimulation
No stimulation 0 to 0.9
1.0 to 2.9
3.0 ~ 4.9
5.0 or higher
0

Elapsed time Example Comparative Example 1 Comparative Example 2 24 hours 0 0.0 0.0 48 hours 0 0.0 0.0

As can be seen from Table 10, skin safety test results show that the average stimulation degree of the Example, Comparative Example 1 and Comparative Example 2 is 0, which is a safe cosmetic without irritation to the skin.

Based on the results of the above experimental examples, cosmetic compositions containing chondroitin sulphate-containing sea cucumber processing by-product extract and hyaluronic acid are prepared and presented. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example  1. Flexible longevity (skin lotion)

Compounding ingredient Weight portion By-product extract of sea cucumber 3.0 Hyaluronic acid 0.1 1,3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenol-9 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example  2. Nourishing lotion ( Milk lotion )

Compounding ingredient Weight portion By-product extract of sea cucumber 5.0 Hyaluronic acid 0.5 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocopheryl acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia nut oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl polymer 1.0 Viechti 0.01 EDITEE-2 EN 0.01 Incense, preservative a very small amount Purified water to 100

Formulation Example  3. Nourishing Cream

Compounding ingredient Weight portion By-product extract of sea cucumber 10.0 Hyaluronic acid 2.0 Cetostearyl alcohol 2.0 Glyceryl stearate 1.5 Trioctanoin 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Liquid paraffin 3.0 Cyclomethicone 3.0 Viechti 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Xanthan gum 0.1 EDITEE-2 EN 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example  4. Massage Cream

Compounding ingredient Weight portion By-product extract of sea cucumber 3.0 Hyaluronic acid 0.3 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Wax 2.0 Tocopheryl acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl alcohol 2.0 Liquid paraffin 30.0 Carboxyvinyl polymer 0.5 Incense, preservative a very small amount Purified water to 100

Formulation Example  5. Pack

Compounding ingredient Weight portion By-product extract of sea cucumber 5.0 Hyaluronic acid 0.5 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl polymer 0.3 ethanol 7.0 Piggy-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Viechti 0.01 EDITEE-2 EN 0.01 Incense, preservative a very small amount Purified water to 100

Claims (5)

A cosmetic composition for improving wrinkles containing a sea cucumber processing by-product extract containing chondroitin sulfate and hyaluronic acid,
Wherein the cosmetic composition comprises 0.5 to 20% by weight of sea cucumber by-product extract and 0.1 to 2.0% by weight of hyaluronic acid based on the total weight of the cosmetic,
Wherein the weight ratio of the sea cucumber processing by-product extract containing chondroitin sulfate to hyaluronic acid is 5: 1 to 30: 1. 1. A whitening cosmetic composition comprising a sea cucumber processing by-product extract containing chondroitin sulfate and hyaluronic acid,
Wherein the cosmetic composition comprises 0.5 to 20% by weight of sea cucumber by-product extract and 0.1 to 2.0% by weight of hyaluronic acid based on the total weight of the cosmetic,
Wherein the weight ratio of the sea cucumber processing by-product extract containing the chondroitin sulfate to the hyaluronic acid is from 5: 1 to 30: 1. A moisturizing cosmetic composition comprising a sea cucumber processing by-product extract containing chondroitin sulfate and hyaluronic acid,
Wherein the cosmetic composition comprises 0.5 to 20% by weight of sea cucumber by-product extract and 0.1 to 2.0% by weight of hyaluronic acid based on the total weight of the cosmetic,
Wherein the weight ratio of the sea cucumber processing by-product extract containing chondroitin sulfate to hyaluronic acid is 5: 1 to 30: 1. CLAIMS 1. A cosmetic composition for enhancing skin elasticity comprising a sea cucumber processing by-product extract containing chondroitin sulfate and hyaluronic acid,
Wherein the cosmetic composition comprises 0.5 to 20% by weight of sea cucumber by-product extract and 0.1 to 2.0% by weight of hyaluronic acid based on the total weight of the cosmetic,
Wherein the weight ratio of the sea cucumber processing by-product extract containing chondroitin sulfate to hyaluronic acid is 5: 1 to 30: 1. 5. The method according to any one of claims 1 to 4,
Wherein the sea cucumber processing by-product extract is a hot-water extract of sea cucumber which is prepared by adding sea cucumber into water and heated at a temperature of 60 ° C to 100 ° C for 1 to 6 hours.