KR101224519B1 - Cosmetic composition containing phellinus linteus mycelium ferment collagen filtrate for preventing skin aging and improving skin wrinkle - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Phellinus linteus mycelium-fermented collagen is provided to treat various aging phenomena and to maximize anti-wrinkling, whitening, and moisturizing effects. CONSTITUTION: A method for preparing Phellinus linteus mycelium-fermented collagen comprises: a step of mixing 1.0-30.0 wt% of collagen and 70.0-99.0 wt% of purified water and inputting the mixture into a fermentation culture tank; a step of sterilizing the mixture at 125-135 deg. C for 1-5 minutes; a step of quickly cooling the mixture at 20-25 deg. C and preparing a collagen natural medium; a step of inoculating 5.0-15.0 wt% of Phellinus linteus mycelium to the collagen natural medium; and a step of culturing and fermenting the mycelium-inoculated collagen natural medium at 24-34 deg. C for 3-10 days and filtering. A cosmetic composition for preventing skin aging and improving anti-wrinkling contains 0.01-95.0 wt% of the Phellinus linteus mycelium-fermented collagen.

Description

Situation mushroom mycelium for preventing skin aging and improving wrinkles Cosmetic composition for preventing skin aging and improving wrinkles containing fermented collagen {Cosmetic Composition containing Phellinus Linteus Mycelium Ferment Collagen Filtrate for preventing skin aging and improving skin wrinkle}

The present invention relates to a cosmetic composition containing the mushroom mushroom mycelium fermented collagen, and more particularly, to a cosmetic composition having an excellent effect of improving skin aging phenomenon by containing the mushroom mushroom mycelium fermented collagen.

One of the biggest areas of interest in cosmetics is wrinkles, pigmentation, decreased elasticity, skin drying and hair loss or hair gloss associated with aging. The skin undergoes various changes through aging. First, the components of the skin, such as the epidermis, the dermis, and the subcutaneous tissue, become thinner and the extracellular matrix (ECM), which gives elasticity to the skin, changes. Among them, 70-80% of the extracellular matrix (ECM) The collagen occupies rapidly decreases with age and is closely related to wrinkle formation. Collagen, elastin, proteoglycans, and glucose aminoglycans that form skin connective tissues (glucosaminoglycan), laminin (laminin), fibronectin (fibronectin), etc. are oxidized and lose their function, the skin loses its elasticity and wrinkles are excessively formed, turning into aged skin. The connective tissue fibers of the extracellular matrix (ECM) include glue fibers (collagen fibers, collagen fibers), reticular fibers, and elastic fibers (elastic fibers, elastic fibers). Collagen, which is occupied, is mostly formed in fibroblasts of the skin. As we age, collagen content in skin connective tissues decreases due to a decrease in collagen synthesis and accelerated degradation. Therefore, degradation of collagen biosynthesis and promotion of collagen degradation may be the main cause of skin wrinkle formation. Collagen biosynthesis process is controlled by many factors involved in transcription level and post-translation level, and the collagen breakdown is collagenase that breaks down collagen by UV light. The expression of matrix metalloproteases (MMP), such as Collagenase, promotes collagen breakdown, reducing collagen content. In addition, as the deformation of collagen is accelerated by the external environment, the skin becomes wrinkled and deepened. As a result, skin aging is manifested by cell homogenization, loss of elastin, destruction of collagen, reduction and delay in collagen synthesis. Thus, skin aging occurs in the skin epidermis, but rather in the dermis.

In particular, most people easily respond to the sun during the growing season, effectively protecting the skin from harmful elements such as ultraviolet rays during the day, but as the age increases, the skin tissue gradually changes, which lowers the ability to defend against the sun. Wrinkles, pigmentation, sagging of the skin, loss of skin elasticity, roughness of the skin, dryness of the skin, hair loss and reduced hair shine. The accumulation of these effects of sunlight is sometimes called photoaging.

Various cosmetic compositions have been studied to solve the problems of skin aging, and the wrinkle improvement effect of the skin has shown visible results in some. Retinoids are widely used to improve skin wrinkles, blemishes, and pigmentation. Among them, clinical results of skin wrinkle removal for retinol have been reported. Retinol-containing cosmetics have wrinkles or skin formed by sunlight. Sag and elasticity were effectively improved. In US Patent Nos. 4,603,146 and 4,877,805, there are cases of wrinkle improvement using retinol (vitamin A), which is effective in collagen synthesis and inhibition of collagen degradation by collagenase activity inhibition.

In addition, it is reported that a cosmetic composition containing L-ascorbic acid (vitamin C), which is essential for the collagen synthesis process, is effective for skin wrinkle improvement, sun protection, pigmentation of spots, freckles, and mushrooms. (US Patent No. 4,938,969 / US Patent No. 4,772,59 / European Patent No. 0533667)

In addition, aha (α-Hydroxy acid: AHA) containing cosmetic composition that is effective in exfoliation has also been widely used to improve the skin by promoting the activity of the cells with the exfoliation.

Recently, collagen itself has been used in cosmetics and has been widely used due to wrinkle improvement and elasticity effect of collagen, but the use of cosmetics has a high concentration, which has a practical effect, which lowers the feeling of use of cosmetics, and has a particular odor problem.

 In order to overcome the problems of the conventional collagen as described above, the present invention has a mechanism to improve the skin aging phenomenon of collagen, while using the collagen to overcome the problems of difficulty in use concentration and specific odor It is an object of the present invention to provide a method for producing fermented collagen of mushrooms from the mushroom mushroom mycelium obtained by fermentation.

 In addition, another object of the present invention is to provide a cosmetic composition that improves the skin aging phenomenon is not sticky and has no specific odor regardless of the concentration used by containing the situation mushroom mycelium fermented collagen.

An object of the present invention as described above, 1.0 to 30.0% by weight of collagen and 70.0 to 99.0% by weight of purified water was added to the fermentation culture tank and heat sterilized for 1 to 5 minutes at a temperature of 125 to 132 ℃, 20 to 25 ℃ Quenching to room temperature to prepare a collagen natural medium; Inoculating the collagen natural medium 5.0 to 15.0% by weight of the situation mushroom mycelium to the weight of the natural medium; And the cultured mushroom mycelium fermented collagen comprising the step of culturing and fermenting the natural collagen medium inoculated with the situation mushroom mycelium for 3 to 10 days at a temperature of 24 to 34 ° C. and aeration of 0.4 to 0.8 vvm It is achieved by the manufacturing method of.

When preparing the collagen natural medium in the above method, by heat sterilization for a short time of 1 to 5 minutes at a high temperature of 125 to 135 ℃, it can significantly reduce the specific odor of collagen.

Here, preferably, the collagen uses low molecular weight collagen having a molecular weight of 10,000 or less extracted from fish.

Preferably, the situation mushroom mycelium uses a mycelium obtained by culturing for 5 to 7 days at a temperature of 28 to 30 ℃, aeration amount of 0.5 to 0.6 vvm.

In addition, it is an object of the present invention to prevent the skin aging and skin containing the mushroom mushroom mycelium fermented collagen prepared by the production method 0.01 to 95.0% by weight, preferably 0.01 to 20.0% by weight relative to the total weight of the cosmetic composition It is achieved by a cosmetic composition for improving wrinkles.

Here, the formulation of the cosmetic composition containing the mushroom mushroom mycelium fermented collagen is softening longevity, astringent longevity, nourishing longevity, eye cream, serum, nutrition cream, massage cream, cleansing cream, cleansing lotion, cleansing foam, cleansing water, powder , Essence, pack, hair tonic, hair treatment, shampoo, rinse.

The cosmetic composition containing the situation mushroom mycelium fermented collagen of the present invention allows the collagen to overcome the difficulties of use due to the high concentration of use and the specific smell while improving the skin aging phenomenon. In addition, even at low concentrations it provides a cosmetic that can effectively improve various skin aging phenomena, such as skin wrinkle improvement, whitening, elasticity, moisturizing and hair loss prevention, hair flexibility enhancement.

The present invention relates to a cosmetic composition containing the situation mushroom mycelium fermented collagen relates to a cosmetic composition excellent in improving the skin aging phenomenon.

Fermented collagen of mycelium mushroom mycelium of the present invention is obtained by inoculating the mycelium mushroom mycelium on a natural medium containing collagen as a main ingredient as a substrate and culturing and fermenting it.

The collagen used in the present invention is low molecular weight collagen having a molecular weight of 10,000 or less extracted from fish. Collagen, also known as collagen, is a light protein that makes up the scales of fish in addition to bones, cartilage, teeth, and skin of animals. It is present as a fibrous solid and has a complicated horizontal pattern structure under an electron microscope. It is insoluble in alkali, but when boiled it becomes gelatin and dissolves. The constituent amino acids are proline, oxyproline, glycine, glutamic acid and the like, and among them, the content of oxyproline which is not present in other proteins is high. Trypsin and other proteolytic enzymes are hardly received, and are under the action of collagenase. Separation from tissues is performed by trypsin and hyaluronidase after organic solvent extraction, acid and alkali treatment to obtain insoluble matter as collagen. Collagen molecule is a collagen structure in which two polypeptide chains are wound and bonded to each other by hydrogen bond. The basic structural unit of collagen is tropocollagen, which has a triple helix structure consisting of three polypeptides of about 100,000 molecular weight. As a constituent amino acid, it contains a great deal of glycine and proline. Our body consists of about 60 trillion cells, and collagen is an important component that fills the cells. Collagen is also a protein that acts like an adhesive to keep cells and cells from falling inside your body. It is an important ingredient that makes up one third of the proteins that make up our body. Each part of our body is composed of collagen. Keratin, the tissue of the hair, is structurally similar to collagen, and the main component of the cornea and conjunctiva of the eye is collagen. 18% of teeth dentin and gums and the fascia are mainly collagen. 70% of the dermis in the skin is collagen, and keratin, the main ingredient of nails and toenails, is structurally similar to collagen. In addition, 50% of the cartilage that connects the bone is collagen, and 80% of the bone organic matter is collagen.

Collagen is deeply related to aging, and as the body ages, collagen biosynthesis ability decreases, causing a variety of aging phenomena. By the age of 18, biosynthesis starts to fall rapidly. At age 40, they are less than half of those at age 18, showing various metabolic difficulties due to lack of collagen content. This lack of collagen content in the human body first causes wrinkles on the face or decreases the elasticity of the skin, pigmentation occurs on the skin, dull skin tone, and speeds up hair loss. Like the skin, the hair contains collagen, so if the collagen is insufficient, the hair will fall out, and the proper supply of collagen will help prevent hair loss and keep the hair shiny. Therefore, many researchers use collagen to improve various skin aging phenomena, but there are difficulties in using due to the specific odor that collagen itself has, and especially the high concentration of collagen required for improving skin aging phenomenon of collagen. The present inventors have a problem by fermenting collagen with a situation mushroom mycelium to remove the specific odor of collagen as well as to enhance the effect.

Fermented collagen of the situation mushroom mycelium of the present invention is a natural medium containing 1.0 to 30.0% by weight of collagen as a substrate, inoculated with 5.0 to 15.0% by weight of the situation mushroom mycelium to the weight of the natural medium, the situation mushroom mycelium The inoculated collagen natural medium was cultured and fermented for 3 to 10 days at a temperature of 24 to 34 ℃, aeration 0.4 to 0.8vvm, and then filtered to prepare fermented collagen of the situation mushroom mycelium of the present invention. The situation mushroom mycelium was classified by identifying and identifying the Korean situation mushroom fruiting body was incubated for 7 days at 30 ℃ in PDA medium, mycelium from the culture was used as a spawn. The natural medium containing the collagen as a main component, mixed with 1.0 to 30.0% by weight of collagen and 70.0 to 99.0% by weight of purified water was added to the fermentation broth and mixed, and then heat sterilized for 1 to 5 minutes at a temperature of 125 to 132 ℃, 20 Prepared by quenching at room temperature to 25 ℃. In this way, by heat sterilization for a short time of 1 to 5 minutes at a high temperature of 125 to 135 ℃, the specific odor of collagen can be significantly reduced.

In order to achieve the desired effect in the present invention, the situation mushroom mycelium fermented collagen is contained in an amount of 0.01 to 95.0% by weight, preferably 0.1 to 20.0% by weight based on the total weight of the composition. In addition, the cosmetic composition containing the situation mushroom mycelium fermented collagen of the present invention is not particularly limited in the formulation, for example, supple cosmetics, astringent cosmetics, nourishing cosmetics, eye cream, serum, nutrition cream, massage cream, It may have a formulation such as cleansing cream, cleansing lotion, cleansing foam, cleansing water, powder, essence, pack, hair tonic, hair treatment, shampoo, rinse and the like. In addition, in the cosmetic composition of each formulation, other ingredients for further enhancing the efficacy of the situation mushroom mycelium fermented collagen in addition to the above-mentioned mushroom mushroom mycelium fermented collagen may be appropriately selected and blended by those skilled in the art without difficulty according to the formulation or purpose of use of other cosmetics. Can be.

Hereinafter, the configuration and effects of the present invention will be described in detail with reference to Examples. However, these examples are only provided to those skilled in the art to more clearly describe the present invention, and the present invention is not intended to be limited to these examples, embodiments according to the present invention are various It may be modified in other forms. For example, a composition containing the mushroom mushroom mycelium fermented collagen and the mushroom mushroom mycelium fermented collagen of the present invention and a composition containing collagen were prepared.

[ Example ]

◎ Method for preparing fermented collagen of S. mushroom mycelium

The situation mushroom mycelium fermented collagen used in the embodiment of the present invention is based on a natural medium containing 10.0% by weight of collagen as a substrate, and inoculated with 10.0% by weight of the situation mushroom mycelium relative to the weight of the natural medium, the situation mushroom mycelium The inoculated collagen natural medium was cultured and fermented at a temperature of 25 ° C. and aeration rate of 0.5 vvm for 5 days, followed by filtration to prepare fermented collagen of the situation mushroom mycelium of the present invention. The situation mushroom mycelium was classified by identifying and identifying the Korean situation mushroom fruiting body was incubated for 7 days at 30 ℃ in PDA medium, mycelium from the culture was used as a spawn. The natural medium containing the collagen as a main component was prepared by mixing 10.0% by weight of collagen and 90.0% by weight of purified water, adding the mixture to a fermentation broth, followed by heat sterilization at a temperature of 125 ° C. for 1 minute, and quenching at room temperature.

◎ Situation mushroom mycelia according to the present invention fermented collagen and containing collagen Cosmetics  Method of Preparation of the Composition

Situation mushroom mycelium fermented collagen (Example) and collagen (comparative example) using the collagen (comparative example) used in the manufacture of natural collagen medium prepared by the method described in Table 1, 2, 3 A cosmetic composition containing collagen was prepared. At this time, in order to make the collagen content in the collagen content in the fermented collagen (collagen weight%) of the example and the collagen content of the comparative example was prepared by adjusting the collagen content in the cosmetic composition by weight% unit. Table 1 is a cream formulation containing the situation mushroom mycelium fermented collagen, Table 2 is a hair shampoo, Table 3 is a hair treatment formulation.

Example  1: skin wrinkle improvement

1. Instrumental evaluation

In order to measure the skin wrinkle improvement effect (mechanical evaluation) for the cosmetic composition prepared by the embodiment according to the present invention, 20 women (20 years old or older) divided into two groups A and B, respectively, The cosmetic composition prepared according to the example of Table 1 according to the present invention was applied to the group A and the comparative example to the group B, respectively.

A group of A and B groups were applied to the area of the upper arm 2X2 cm 2 (2 times / day, 0.2 g / time) for 6 weeks, and a replica of the skin wrinkles was made using a transparent silicone solution. Skin wrinkles were measured by a wrinkle measuring instrument (SKIN VISIOMETER SV400, C + K Electronics GmbH. Germany). Three-dimensional analysis of the image of the replica with a CCD camera, wrinkles by the average wrinkle roughness (R _z ) of the following formula 1 which is the sum of the roughness (R _m : m is an integer of 1 or more) of each wrinkle divided by the number of wrinkles The improvement effect was analyzed. The test results are shown in Table 4 below.

[Equation 1]

[Table 4] Skin wrinkle improvement effect (6 weeks)

As can be seen in Table 4, after six weeks the embodiment according to the present invention has an average wrinkle roughness (R _z ) is 88.7㎛ (p <0.01), Comparative Example is 64.6㎛ (p <0.01), The cosmetics containing the situation mushroom mycelium fermented collagen according to the present invention improved about 37.3% more wrinkles than the comparative example.

Therefore, it can be seen that the cosmetic composition containing the situation mushroom mycelium fermented collagen according to the present invention has a very effective wrinkle improvement effect.

2. Visual Evaluation

In order to measure the skin wrinkle improvement effect (visual evaluation) on the cosmetic composition prepared by the embodiment according to the present invention, 20 women (20 years old or older) were divided into two groups, A and B, respectively. The cosmetic composition prepared according to the example of Table 1 according to the invention was applied to the group A and the comparative example to the group B, respectively.

After six weeks of application (2 times / day) around wrinkled eyes on each subject of group A and B, improvement of skin wrinkles was classified by evaluating subject's subjective evaluation and subject's subjective evaluation into the following six levels. Degree (ΔW) was measured. The results are shown in Tables 5 and 6 below.

<Evaluation criteria for skin wrinkle improvement>

-3: extremely badly -2: worsening -1: slightly worsening

0: no change 1: slightly improved 2: improved 3: highly improved

[Table 5] Clinical Effects of Skin Wrinkle Improvement (Objective Evaluation by Skilled Workers)

[Table 6] Clinical Effects of Skin Wrinkle Improvement Subject's  Subjective evaluation)

As can be seen in Table 5 and Table 6, in the embodiment containing the situation mushroom mycelium fermented collagen according to the present invention, the objective evaluation of the expert and the subjective evaluation of the subject were 2.5 and 2.6, respectively, It was found that 25.0% in the objective evaluation and 23.8% in the subjective evaluation of the subject showed that the cosmetics containing fermented collagen of mycelium mushroom mycelium improved the wrinkles more effectively than the cosmetics containing collagen.

Example  2: skin whitening effect ( From Melanosite  Inhibitory effect on melanin production)

Melanosite was used by purchasing a mouse-derived B-16 melanoma (ATCC CRL 6323) cell line. Melanoma cell lines were inoculated in DMEM medium containing 4.5 g / L glucose, 10% serum and 1% antibiotics and incubated at 37 ° C. in a 50 ml T-flask. 5% CO ₂ After culturing for 24 hours under the conditions, cells were isolated by treatment with 0.05% Trypsin containing 0.02% EDTA, and then inoculated in a 50 ml T-flask and incubated for 48 hours. At this time the cell number is 5.76 × 10 ⁶ cells / flask. Here, the mushroom fungus mycelium fermented collagen and collagen of the embodiment of the present invention in an appropriate concentration are diluted in DMEM medium and treated with cultured melanoma cells and cultured at 37 ° C. for 5 days. After incubation, all of the medium was removed, cells were treated with 1 ml of saline-phosphate buffer (PBS) containing 0.02% EDTA and 0.05% trypsin, and then cells were collected by centrifugation for 5 minutes. 5% trichloroacetate (TCA) is treated, stirred and centrifuged to wash the precipitated melanin with saline-phosphate buffer. 1N NaOH was dissolved in the melanin precipitated, and then the absorbance was measured at 475 nm. Melanin concentrations were determined from standard concentration curves of synthetic melanin (Sigma). The experimental results are shown in Table 7.

[Table 7] From Melanosite  Melanin Synthesis Inhibitory Effect

As can be seen in Table 7, the situation mushroom mycelium fermented collagen of the embodiment according to the present invention showed a higher melanin synthesis inhibitory effect than when treated with collagen of the comparative example, the situation mushroom mycelium fermented collagen of the present invention is melanin of melanocytes It can be seen that the skin whitening effect by inhibiting the production.

Example  3: improvement of skin elasticity

The skin elasticity improvement effect was measured for the cosmetic example containing the situation mushroom mycelium fermented collagen and the comparative example containing the collagen of the present invention.

In the temperature 24-26 ℃, humidity of 45% conditions 20 women over 20 years (average age 28.5 years) divided into two groups A and B, respectively, the cosmetic composition prepared by the Example of Table 1 according to the present invention Comparative Example to Group A was applied to Group B each eye for 6 weeks (2 times / day), and then skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., Germany). Measured. The test results are described in Table 8 below as the ΔR (R (before use) -R8 (after use)) values of the Cutometer SEM 575. R values indicate the nature of skin viscoelasticity.

Table 8 Skin Elasticity (After 6 Weeks)

As can be seen from the results in Table 8, the cosmetic example containing the situation mushroom mycelium fermented collagen increased the skin elasticity by 37.5% compared with the comparative example.

Example  4: moisturizing effect

The skin moisturizing effect of the cosmetic example containing the situation mushroom mycelium fermented collagen and the comparative example containing the collagen of the present invention was measured. The measurement was performed after stabilizing the skin for 20 to 30 minutes in a room having a temperature of 24-26 ° C., a relative humidity of 45%, and no air flow. Twenty women 20 years or older (average age 28.5 years) were divided into groups A and B, respectively, and the cosmetic composition prepared according to the embodiment of the present invention was in A group, and the cosmetic composition prepared in Comparative Example was in B group. After 6 weeks of application (2 times / day) around the eyes, TEWAMETER TM210 (C + K electronic GmbH. Germany) was used to change the transdermal moisture loss, that is, TEWL (Transepidermal Water Loss) Measured by △ TEWL, using the CORNEOMETER CM 820 PC (C + K electronic GmbH. Germany) to measure the moisturizing effect by quantifying the moisture content of the skin from 0 to 150 by the change of electrical conductivity according to the epidermal moisture content. The test results are shown in Table 9 below.

TABLE 9 Skin moisturizing effect

As a result of the test, it can be seen that the ΔTEWL value of the Example was 5.5g / hm ² , and the transdermal moisture loss amount was effectively improved after 6 weeks compared to the comparative example.

In addition, the corneometer value, which measures the moisture content of the skin, can be seen that the Examples are increased by 23.3% compared to the Comparative Examples, respectively, and the skin becomes very moist.

Example  5: stability of formulation and Peculiarity  exam

The stability test of the formulation for the cosmetic containing the situation mushroom mycelium fermented collagen was conducted. In order to test the stability of the cosmetics in Example 1 and Comparative Examples in Table 1 and 4 ℃ and 45 ℃ in a constant temperature bath kept in a transparent glass container for 4 weeks, respectively stored after separation, discoloration and odor (specific odor ) Degree was compared and measured. The results are shown in Table 10 below. At this time, the product separation, discoloration, and the degree of odor were classified into the following six ratings.

Product discoloration evaluation criteria:

0: No change

1: very little separation (color change, odor)

2: a little separation (color change, deodorization)

3: Slightly severe separation (discoloration, odor)

4: severely separated (color change, deodorant)

5: extremely severe separation (discoloration, deodorization)

[Table 10] Separation

As can be seen in Table 10, the embodiment containing the situation mushroom mycelium fermented collagen of the present invention can be seen that there is no separation, discoloration and discoloration (special odor) phenomenon is a stable base. On the other hand, the comparative example containing collagen has a slight discoloration symptom, and it can be seen that the odor (specific odor) phenomenon occurs a bit severely and has a problem in stability.

Example  6: hair loss prevention and hair softening effect

Hair loss prevention and hair flexibility test of the formulation for the cosmetic containing the situation mushroom mycelium fermented collagen was performed. In order to measure hair loss prevention and hair flexibility of cosmetics, 20 women over 20 who are suffering from hair loss through a questionnaire (average age 43.5 years) are divided into two groups A and B, respectively, the hair shampoo of Table 2 according to the present invention. The hair treatment examples of Table 3 and the comparative example in the group A were to be used for 6 weeks (once / day) in the group B, respectively. The method of use is to first wash the hair with a hair shampoo and then use the hair treatment to dry the hair and scalp properly.

After six weeks of application (one time / day) to the subjects of groups A and B, hair loss prevention and hair flexibility were measured by classifying the objective evaluation of the skilled person and the subjective evaluation of the subject into the following six levels. The results are shown in Tables 11 and 12 below.

<Evaluation Criteria>

-3: extremely badly -2: worsening -1: slightly worsening

0: no change 1: slightly improved 2: improved 3: highly improved

[Table 11] Clinical Effects of Hair Loss Prevention and Hair Flexibility (Objective Evaluation by Skilled Workers)

[Table 12] Clinical Effects of Hair Loss Prevention and Hair Flexibility (Subjective Evaluation of Tests)

As can be seen in Table 11 and Table 12, the objective evaluation of hair loss prevention and hair flexibility by the skilled person of the embodiment containing the situation mushroom mycelium fermented collagen according to the present invention is 100% hair loss prevention compared to the comparative example, hair Increasing flexibility 63.6%, subjective evaluation by subjects showed that hair loss prevention 150%, hair flexibility 90.9% increased, the condition mushroom mycelium fermentation collagen-containing cosmetics of the present invention improved the hair loss prevention and hair flexibility than cosmetics containing collagen It can be seen.

Hereinafter, based on the results of the above test examples, to present a cosmetic composition of the present invention containing the situation mushroom mycelium fermented collagen. However, the composition of the present invention is not intended to be limited to the following formulation examples.

[ Formulation Example  1] Flexible Cosmetic (Skin Lotion)

[ Formulation Example  2] Nutritional Cosmetics ( Milk lotion )

[ Formulation Example  3] Nutrition Cream

[ Formulation Example  4] Massage Cream

[ Formulation Example  5] pack

Claims (7)

1.0 to 30.0% by weight of collagen and 70.0 to 99.0% by weight of purified water were added to a fermentation vessel, and heat sterilized at a temperature of 125 to 132 ° C. for 1 to 5 minutes, followed by rapid cooling to room temperature of 20 to 25 ° C. to give a collagen natural medium. Preparing;
Inoculating the collagen natural medium 5.0 to 15.0% by weight of the situation mushroom mycelium to the weight of the natural medium; And
The situation mushroom, characterized in that it comprises a step of culturing and fermenting the natural collagen medium inoculated with the situation mushroom mycelium for 3 to 10 days at a temperature of 24 to 34 ℃, aeration amount of 0.4 to 0.8 vvm, and filtering Method for producing mycelium fermented collagen. The method of claim 1, wherein the collagen is a method for producing fermented collagen of the situation mushroom mycelium, characterized in that using a low molecular weight collagen with a molecular weight of 10,000 or less extracted from fish. The method of claim 1, wherein the mycelium mushroom mycelium prepared by the mycelium mushroom mycelium fermented collagen characterized in that the mycelium obtained by culturing for 5 to 7 days at a temperature of 28 to 30 ℃, aeration amount of 0.5 to 0.6 vvm using Way. Claims 1 to 3 of the situation mushroom mycelium mycelium fermented collagen prepared by the method of any one of the composition for the prevention of skin aging and wrinkle improvement, characterized in that it contains 0.01 to 95.0% by weight relative to the total weight of the cosmetic composition Cosmetic composition. For preventing skin aging and improving wrinkles, the fermented collagen of mushrooms prepared by the method according to any one of claims 1 to 3 contains 0.01 to 20.0% by weight based on the total weight of the cosmetic composition. Cosmetic composition. According to claim 4, wherein the formulation of the cosmetic composition containing the mushroom mushroom mycelium fermented collagen is softening longevity, astringent longevity, nutrient longevity, eye cream, serum, nutrition cream, massage cream, cleansing cream, cleansing lotion, cleansing foam, cleansing Water, powder, essence, pack, hair tonic, hair treatment, shampoo, rinse a cosmetic composition for preventing skin aging and wrinkles, characterized in that the formulation. According to claim 5, wherein the formulation of the cosmetic composition containing the mushroom mushroom mycelium fermented collagen is softening longevity, astringent longevity, nourishing longevity, eye cream, serum, nutrition cream, massage cream, cleansing cream, cleansing lotion, cleansing foam, cleansing Water, powder, essence, pack, hair tonic, hair treatment, shampoo, rinse a cosmetic composition for preventing skin aging and wrinkles, characterized in that the formulation.