KR20170123079A - Method for improving growth and phytochemicals of Crepidiastrum plants using various LED lights - Google Patents

Method for improving growth and phytochemicals of Crepidiastrum plants using various LED lights Download PDF

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KR20170123079A
KR20170123079A KR1020160052279A KR20160052279A KR20170123079A KR 20170123079 A KR20170123079 A KR 20170123079A KR 1020160052279 A KR1020160052279 A KR 1020160052279A KR 20160052279 A KR20160052279 A KR 20160052279A KR 20170123079 A KR20170123079 A KR 20170123079A
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KR101861217B1 (en
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오명민
박송이
배지훈
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충북대학교 산학협력단
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    • A—HUMAN NECESSITIES
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Abstract

The present invention relates to a method for increasing bioactive substances and growth of Crepidiastrum denticulatum using various light-emitting diodes (LEDs). By securing an optimized LED technology to increase the bioactive substances and growth of Crepidiastrum denticulatum which is a medicinal crop used as a raw material for drugs, the method of the present invention can be applied as a technology to produce highly functional plant raw materials.

Description

다양한 LED를 이용한 고들빼기속 작물의 생장 및 생리활성 물질 증진 방법{Method for improving growth and phytochemicals of Crepidiastrum plants using various LED lights}FIELD OF THE INVENTION [0001] The present invention relates to a method for enhancing the growth of plants and a physiologically active substance using various LEDs,

본 발명은 식물공장에서 고기능성 고들빼기 속 작물 이고들빼기의 생장과 생리활성 물질 증대 기술 개발을 위하여, 다양한 LED 광질을 이용하여 이고들빼기의 최적 광질 조건을 구명하고 인공광 환경제어 기술을 확립한 것에 관한 것이다. The present invention relates to a high-performance grape seedling plant in a plant, and to develop a technology for increasing the amount of bioactive substances and extracting the seeds, .

식물이 광합성을 하는 경우 생육을 위해 사용하는 빛은 특정 파장 영역으로 제한되어 있다. 그러나 인공조명의 대부분은 인간의 시감을 위해 개발되었기 때문에 같은 양의 밝기라도 정착 식물에 유효한 빛은 아주 적은 편이다. 예를 들어 최근에 도시화 농업을 이끌고 있는 식물공장에 도입되는 인공조명은 지구 온난화의 주범인 온실가스 저감을 위해 에너지 소비를 최소화하고 식물 생육활동에 있어 광합성 유효파장의 최대효율 및 광 형태 형성에 관여하는 광색 밸런스로 나타내는 파장 대역을 내는 인공 광원이 필요하다. 최근에는 그동안 사용되어 온 백열등 또는 형광등에 비하여 특정 파장역만을 선택적으로 사용할 수 있는 광효율이 높은 친환경 녹색조명인 LED(Light Emitting Diode) 조명을 활용한 식물 생육 환경을 구현하고 있다.When plants are photosynthetic, the light used for growth is limited to specific wavelengths. However, since most of the artificial lighting was developed for human vision, the same amount of light is very light available to the settled plants. For example, artificial lighting, which has recently been introduced into plant factories leading to urbanization agriculture, minimizes energy consumption for greenhouse gas reduction, which is the main cause of global warming, and is involved in the maximum efficiency of photosynthesis effective wavelength and formation of optical form in plant growth activity An artificial light source that emits a wavelength band represented by a light color balance is required. In recent years, a plant growth environment utilizing LED (Light Emitting Diode) lighting, which is an eco-friendly green light with high light efficiency, which can selectively use only a specific wavelength region compared to an incandescent lamp or a fluorescent lamp which has been used in the past, is implemented.

LED(light emitting diodes)는 백열등에 비해 전환이 빠르며, 낮은 에너지소비, 긴 수명, 소형, 내구성 및 신뢰성 등의 여러 가지 장점을 지니고 있어서, 식물의 광 형태 형성 및 생장을 조절하기 위한 광원으로 사용하고자 하는 많은 연구가 진행되고 있다(Heo, 2002 Plant Growth Regulation 38 : 225-230).Light emitting diodes (LEDs) have advantages over light incandescent lamps, low energy consumption, long lifetime, compactness, durability and reliability, so that they can be used as a light source to control the formation and growth of optical shapes of plants. (Heo, 2002 Plant Growth Regulation 38: 225-230).

식물의 생육에 영향을 미치는 광 환경을 보자면 광도(light intensity), 광질(light quality) 및 일장(daylength)이 있다. 광합성은 빛의 광도(light intensity)에 영향을 받으며, 적색광 및 청색광의 파장대가 식물의 생육 등에 효과적이다. 식물은 자외선이나 가시광선 영역 중 특정 파장에 의하여 광합성이 촉진되기도 하고 형태적인 형성이 이루어지기도 한다. 특히 청색광(400-500nm)은 광합성을 촉진하고 줄기 신장을 억제하며 적색광(600-700nm)은 광합성 촉진, 개화 및 줄기신장에 관여하고 녹색광(500-600nm)은 광합성의 보조역할을 한다고 알려져 있다. 그리고 원적색광(700-800nm)은 개화, 줄기신장 촉진, 종자 발아조절에 관여하고 자외선 A 및 B는 피토토케이컬(Phytochemicals) 합성에 관여한다고 밝혀졌다. 또한 식물은 일장에 따라 개화시기가 결정되기도 한다.There are light intensity, light quality and daylength in terms of the optical environment that affects plant growth. Photosynthesis is influenced by the light intensity of light, and the wavelength range of red light and blue light is effective for the growth of plants. Plants may be stimulated by photosynthesis or morphologically formed by specific wavelengths in the ultraviolet or visible range. In particular, blue light (400-500nm) promotes photosynthesis and suppresses stem elongation, while red light (600-700nm) is involved in photosynthesis promotion, flowering and stem elongation, and green light (500-600nm) is known to be a secondary function of photosynthesis. It has been found that the red light (700-800 nm) is involved in flowering, promoting stem elongation, controlling seed germination, and ultraviolet rays A and B are involved in the synthesis of phytochemicals. Plants are also determined by the time of flowering time.

이고들빼기(Crepidiastrum denticulatum)는 쌍떡잎식물 합판화군 초롱꽃목 국화과의 한해살이 또는 두해살이풀로 산과 들의 건조한 곳에서 자라며, 높이 30∼70㎝이고, 줄기는 가늘고 자줏빛이다. 가지가 퍼지며 자르면 즙이 나온다. 뿌리에 달린 잎은 주걱 모양이며 꽃이 필 때 쓰러지고 줄기에 달린 잎은 어긋나며 잎자루가 없다. 잎 길이는 6-11㎝, 나비는 3-7㎝이며 끝은 둔하다. 밑부분은 귀처럼 되어 줄기를 반쯤 감싸고, 가장자리에 이 모양의 톱니가 드문드문 있다. 꽃은 8-9월에 노란색으로 피고 두화는 지름 15㎜ 정도로서 산방꽃차례로 달리는데, 꽃이 필 때는 곧게 서고 진 다음 밑으로 처진다. 총포는 좁은 통처럼 생기고 총포조각은 긴 타원 모양 바소꼴로서 2줄로 늘어서고, 안조각은 줄 모양이며 8개이다. 열매는 수과(瘦果)로서 갈색이나 검은색이며 12개의 능선이 있다. 관모는 흰색이며 길이 약 3.5 ㎜이며, 어린순을 나물로 먹고, 한국·일본·중국·인도차이나에 분포하며 다양한 기능성 물질을 포함하고 있다고 알려져있다. Crepidiastrum denticulatum ) is a perennial plant or a biennial plant of the dicotyledonous plants of the dicotyledonous plants. It grows in the dry places of the mountains and is 30-70 cm high. The stem is thin and purple. Branches spread and juice comes out. The leaves on the roots are shaped like spatulas and fall when flowers are bloomed. The leaves on the stem are alternate and have no petiole. Leaf length is 6-11 ㎝, butterfly is 3-7 ㎝, end is dull. The lower part is like an ear, and half of the stem is wrapped, and the saw teeth on the edge are sparsely scattered. The flower blooms in August-September with yellow color. The flower is 15㎜ in diameter and runs in the flower bed. When the flower is used, it stands straight and then falls down. The gun guns are like narrow cylinders, and the gun sculptures are long oval-shaped bar-shaped ones arranged in 2 lines. Fruits are brown or black with 12 ridges. The tubular is white and has a length of about 3.5 ㎜. It is known that it is distributed in Korea, Japan, China, Indochina and contains various functional materials.

한국 공개 특허 2013-0051846Korean Patent Publication No. 2013-0051846

이에 본 발명자들은 의약기반 원료로 사용하는 약용작물인 이고들빼기의 생장과 생리활성 물질을 증대시키기 위한 방법을 연구하던 중, 최적의 LED 기술을 이용하여 재배하면 고기능성 식물 원료를 생산할 수 있다는 것을 확인함으로써 본 발명을 완성하였다.Accordingly, the inventors of the present invention have been studying a method for enhancing the growth and physiologically active substance of a medicinal plant used as a medicinal-based raw material, and it has been reported that cultivation using an optimal LED technology can produce a high- The present invention has been completed.

따라서 본 발명의 목적은 LED(Light Emitting Diode) 광을 이용한 이고들빼기(Crepidiastrum denticulatum)의 생장 및 생리활성 물질 증진 방법을 제공하는 것이다.Accordingly, an object of the present invention is to provide a method for growing Crepidiastrum denticulatum and promoting physiologically active substances using LED (Light Emitting Diode) light.

또한, 본 발명의 다른 목적은 상기 방법을 이용하여 생장 및 생리활성 물질이 증진된 이고들빼기를 제공하는 것이다. It is a further object of the present invention to provide a method of growing and physiologically active substances using the method, wherein the growth and the bioactive substance are enhanced.

상기와 같은 본 발명의 목적을 달성하기 위하여, 본 발명은 LED(Light Emitting Diode) 광을 이용한 이고들빼기(Crepidiastrum denticulatum)의 생장 및 생리활성 물질 증진 방법을 제공한다. According to an aspect of the present invention as described above, the present invention is used and the subtraction (the Crepidiastrum (Light Emitting Diode) LED light denticulatum ) and a method for promoting bioactive substances.

본 발명의 일실시예에서, 상기 LED 광은 White(W), Red(R), Green(G) 또는 Blue(B)이고, 상기 LED 광은 R:B 6:4, R:B 7:3, R:B 8:2, R:B 9:1, RGB 5:1:4, RGB 6:1:3, RGB 7:1:2, RGB 8:1:1, RGB 9:1:0, RWB 7:1:2, RWB 8:1:1, RWB 6:2:2, RWB 7:2:1 및 RWB 8:2:0으로 이루어진 군에서 선택된 1종 이상의 혼합 LED 광 일 수 있다.In one embodiment of the present invention, the LED light is white (W), red (R), green (G) or blue RGB 6: 1: 3, RGB 7: 1: 2, RGB 8: 1: 1, RGB 9: 1: 0, R: B 8: 2, R: B 9: RWB 7: 2: 1, and RWB 8: 2: 0. The LED light may be at least one selected from the group consisting of RWB 7: 1: 2, RWB 8: 1: 1, RWB 6: 2: 2, RWB 7:

본 발명의 일실시예에서, 근적외선을 추가적으로 조사할 수 있다. In one embodiment of the present invention, near infrared rays can be additionally inspected.

본 발명의 일실시예에서, 근적외선에 대한 적생광에 대한 비율(R/FR)은 0.7, 1.2, 4.1 및 8.6으로 이루어진 군에서 선택된 1종 이상인 것일 수 있다. In one embodiment of the present invention, the ratio (R / FR) to the red light for near infrared rays may be at least one selected from the group consisting of 0.7, 1.2, 4.1 and 8.6.

본 발명의 일실시예에서, 생장은 생체중, 건물중, 엽수, 엽면적, 엽장 및 엽폭으로 이루어진 군에서 선택된 1종 이상이 증가하는 것일 수 있다. In one embodiment of the present invention, the growth may be one or more selected from the group consisting of fresh weight, dry weight, leaf number, leaf area, leaf length and leaf width.

본 발명의 일실시예에서, 생리활성 물질은 페놀 화합물, 치커리산(chicoric acid), 카프타릭산(caftaric acid), 클로로제닉산(chlorogenic acid), 3,5-DCQA(3,5-O-dicaffeoyl-epi-quinic acid) 및 카페익산(caffeic acid)으로 이루어진 군에서 선택된 1종 이상이 증가하는 것일 수 있다. In one embodiment of the present invention, the physiologically active substance is selected from the group consisting of phenolic compounds, chicoric acid, caftaric acid, chlorogenic acid, 3,5-DCQA (3,5-O-dicaffeoyl -epi-quinic acid, and caffeic acid may be increased.

또한, 본 발명은 상기 방법을 이용하여 생장 및 생리활성 물질이 증진된 이고들빼기를 제공한다.The present invention also provides a method for growing and physiologically active substances using the method, wherein the growth and the bioactive substance are enhanced.

본 발명은 의약기반 원료로 사용하는 약용작물인 이고들빼기의 생장과 생리활성 물질을 증대시키기 위한 최적의 LED 기술을 확립하였으므로 고기능성 식물원료 생산 기술로 활용될 수 있다. The present invention can be utilized as a high-functional plant material production technology since it is a medicinal crop used as a medicament-based raw material and has established an optimal LED technology for growing and extracting physiologically active substances.

도 1은 다양한 LED 광질에 따른 이고들빼기 속 작물의 외형적인 비교사진이다.
도 2는 17가지의 LED 광질을 처리한 이고들빼기 지상부의 생체중(shoot fresh weight)과 건물중(shoot dry weight)을 측정한 결과를 나타낸 그래프이다.
도 3은 17가지의 LED 광질을 처리한 이고들빼기의 엽수, 엽면적, 엽장, 엽폭을 측정한 결과를 나타낸 그래프이다.
도 4는 17가지의 LED 광질을 처리한 이고들빼기의 페놀화합물과 항산화도를 측정한 결과를 나타낸 그래프이다.
도 5는 17가지의 LED 광질을 처리한 이고들빼기의 치커리산과 페닐프로파노이드 물질을 측정한 결과를 나타낸 그래프이다.
도 6은 본 발명에서 이용한 근적외선광 LED(RB 8:2 LED 혼합광, RB 8:2 LED 혼합광에 far-red LED를 추가하여 4가지의 R/FR 비율을 조절한 광)을 나타낸 그래프이다.
도 7은 근적외선광 LED를 각각 처리한 이고들빼기의 지상부의 생체중과 건물중, 엽면적, 엽장, 엽폭 및 엽수를 측정한 결과를 나타낸 그래프이다.
도 8은 근적외선광 LED를 각각 처리한 이고들빼기의 항산화능과 총 페놀화합물을 측정한 결과를 나타낸 그래프이다.
도 9는 나타낸 근적외선광 LED를 각각 처리한 이고들빼기의 치커리산과 페닐프로파노이드 물질을 측정한 결과를 나타낸 그래프이다. FIG. 1 is a photograph of a comparison of the appearance of various crops according to various LED qualities.
FIG. 2 is a graph showing the results of measurement of shoot fresh weight and shoot dry weight of the above-ground portions of 17 light-emitting LEDs. FIG.
FIG. 3 is a graph showing the results of measuring leaf number, leaf area, leaf length, and leaf width of leaves of 17 kinds of LED light quality treated.
FIG. 4 is a graph showing the results of measurement of phenol compounds and antioxidant levels of 17 kinds of LED light treated and removed. FIG.
FIG. 5 is a graph showing the results of measurement of chicarboxylic acid and phenylpropanoid material in 17 light-treated LEDs.
6 is a graph showing a near-infrared light LED (RB 8: 2 LED mixed light and RB 8: 2 LED mixed light with a far-red LED added thereto to adjust four R / FR ratios) used in the present invention .
FIG. 7 is a graph showing the result of measuring the fresh weight, the weight of the building, the leaf area, the leaf area, the leaf width and the leaf number of the ground portion of each of the grains treated with the near infrared ray LED.
8 is a graph showing the results of measuring the antioxidant ability and the total phenol compound of the extracts treated with the near-infrared light LEDs.
FIG. 9 is a graph showing the results of measurement of chicory acid and phenylpropanoid material in the case of treating each of the near-infrared light LEDs shown in FIG.

이하, 본 발명을 상세히 설명한다. Hereinafter, the present invention will be described in detail.

본 발명은 LED(Light Emitting Diode) 광을 이용한 이고들빼기(Crepidiastrum denticulatum)의 생장 및 생리활성 물질 증진 방법을 제공함에 그 특징이 있다. The present invention is characterized by providing a method of growing Crepidiastrum denticulatum and enhancing a physiologically active substance using LED (Light Emitting Diode) light.

본 명세서에서“이고들빼기(Crepidiastrum denticulatum)”는 쌍떡잎식물 합판화군 초롱꽃목 국화과의 식물을 의미한다. 이는 한해살이 또는 두해살이풀로 산과 들의 건조한 곳에서 자라고, 높이 30∼70 ㎝이며, 줄기는 가늘고 자줏빛이다.In this specification, " Crepidiastrum " denticulatum "refers to a plant of the dicotyledonous plant of the dicotyledonous plant. It is an annual or biennial plant that grows in the dry places of mountains and is 30 to 70 cm high. The stem is thin and purple.

본 발명에 있어서, 상기 LED 광은 White(W), Red(R), Green(G) 또는 Blue(B)이고, 상기 LED 광은 R:B 6:4, R:B 7:3, R:B 8:2, R:B 9:1, RGB 5:1:4, RGB 6:1:3, RGB 7:1:2, RGB 8:1:1, RGB 9:1:0, RWB 7:1:2, RWB 8:1:1, RWB 6:2:2, RWB 7:2:1 및 RWB 8:2:0로 이루어진 군에서 선택된 1종 이상의 혼합 LED 광인 것이 바람직하나, 이에 한정되지 않는다.In the present invention, it is preferable that the LED light is at least one selected from the group consisting of R: B6: 4, R: B7: 3, R: RGB 8: 1: 1, RGB 9: 1: 0, RWB 7: 1: 2, RGB 7: But is not limited to, at least one mixed LED light selected from the group consisting of 1: 2, RWB 8: 1: 1, RWB 6: 2: 2, RWB 7: 2: 1 and RWB 8: 2: .

본 발명에 있어서, 근적외선(far red light)을 추가적으로 조사할 수 있다. In the present invention, a far red light can be additionally irradiated.

본 발명에 있어서, 근적외선에 대한 적생광에 대한 비율(R/FR)은 0.7, 1.2, 4.1 및 8.6로 이루어진 군에서 선택된 1종 이상인 것이 바람직하나, 이에 한정되지 않는다. In the present invention, the ratio (R / FR) of the near infrared rays to the red light is preferably at least one selected from the group consisting of 0.7, 1.2, 4.1 and 8.6, but is not limited thereto.

본 발명에 있어서, 생장은 생체중, 건물중, 엽수, 엽면적, 엽장 및 엽폭으로 이루어진 군에서 선택된 1종 이상이 증가하는 것일 수 있다. In the present invention, the growth may be one or more selected from the group consisting of live weight, dry weight, leaf number, leaf area, leaf area and leaf width.

본 발명에 있어서, 생리활성 물질은 페놀 화합물, 치커리산(chicoric acid), 카프타릭산(caftaric acid), 클로로제닉산(chlorogenic acid), 3,5-DCQA(3,5-O-dicaffeoyl-epi-quinic acid) 및 카페익산(caffeic acid)으로 이루어진 군에서 선택된 1종 이상이 증가하는 것일 수 있다.In the present invention, the physiologically active substance is a phenolic compound, chicoric acid, caftaric acid, chlorogenic acid, 3,5-DCQA (3,5-O-dicaffeoyl-epi- quinic acid, and caffeic acid may be increased.

또한, 본 발명은 상기 방법을 이용하여 생장 및 생리활성 물질이 증진된 이고들빼기를 제공한다.The present invention also provides a method for growing and physiologically active substances using the method, wherein the growth and the bioactive substance are enhanced.

상기 이고들빼기는 LED 광 및 LED 혼합광을 조사한 후, 추가적으로 근적외선 광을 조사하여 생체중, 건물중, 엽수, 엽면적, 엽장 및 엽폭이 증가하고, 생리활성물질인 페놀 화합물, 치커리산(chicoric acid), 카프타릭산(caftaric acid), 클로로제닉산(chlorogenic acid), 3,5-DCQA(3,5-O-dicaffeoyl-epi-quinic acid) 및 카페익산(caffeic acid)이 증가 되어 고기능성 식물 원료로 유용하게 이용될 수 있다. The above and the subtraction can be performed by irradiating the LED light and the LED mixed light and then irradiating the near infrared light to increase the weight of the living body, the number of leaves in the building, the leaf area, the leaf area, the leaf width and the leaf width, and the phenolic compound, chicoric acid, , Caftaric acid, chlorogenic acid, 3,5-DCQA (3,5-O-dicaffeoyl-epi-quinic acid) and caffeic acid have been increased, Can be usefully used.

이하, 실시예를 통하여 본 발명을 보다 상세히 설명하고자 한다. 이들 실시예는 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

<< 실시예Example 1> 다양한 LED1> Various LED 광질에In light 따른  Following 이고들빼기의And subtract 생장 확인 Growth Confirmation

온도 20℃, 습도 60%, 이산화탄소 농도 1000ppm, 200μmol/m2/s PPFD 조건의 밀폐형 식물 생산 시스템에서 이고들빼기는 6주 동안 재배되었다. 광질의 조건은 red(R, 654 nm), green(G, 518 nm), blue(B, 455 nm) 또는 red와 blue의 조합, red, green, blue의 조합, 또한 red, white(456 nm+558 nm), blue의 조합된 17가지의 다양한 비율로 혼합한 혼합 LED에서 재배되었다(도 1 참조). 정식 후 6주차에 17가지 처리구의 지상부의 생체중과 건물중, 엽수, 엽면적, 엽장, 엽폭을 측정하였다. 또한, 총 페놀 화합물의 농도, 항산화능력, 치커리산(chicoric acid), 카프타릭산(caftaric acid), 클로로제닉산(chlorogenic acid) 및 3,5-DCQA(3,5-di-O-caffeoylquinic acid)를 분석하였다. In a closed plant production system with a temperature of 20 ° C, a humidity of 60%, a carbon dioxide concentration of 1000 ppm, and a 200 μmol / m 2 / s PPFD condition, draining was grown for 6 weeks. The combination of red, green and blue and red, white (456 nm +), red (blue), and blue (B, 455 nm) 558 nm), and blue (see Fig. 1). On the 6th week after the planting, the fresh weight, the number of leaves, leaf area, leaf area, leaf width and leaf width of the above - The concentration of total phenolic compounds, antioxidant capacity, chicoric acid, caftaric acid, chlorogenic acid and 3,5-di-O-caffeoylquinic acid (3,5-DCQA) Respectively.

그 결과, 정식 후 6주차 단색광에서 지상부의 생체중과 건물중은 green과 blue광에서 증가했으며 RGB 혼합광에서는 RGB 6:1:3 처리구는 가장 높은 값을 보였다(도 2 참조). As a result, in the monochromatic light at the 6th week after the planting, the live weight and the weight of the ground were increased in the green and blue light, and the RGB 6: 1: 3 treatment was the highest value in the RGB mixed light (see FIG.

또한, 엽수는 RWB 6:2:2에서 가장 많았고 엽면적은 지상부의 생체중과 건물중 결과와 비슷하게 단일광에서는 green과 blue, RGB 혼합광에서는 RGB 6:1:3에서 가장 높았다(도 3 참조). The number of leaves was the highest at RWB 6: 2: 2, and the leaf area was the highest at green and blue for single light and 6: 1: 3 for RGB mixed light, similar to the live weight and building results at the ground level (see FIG.

또한, 총 페놀화합물의 농도와 항산화도는 형광등과 RGB 5:1:4에서 가장 높았지만 식물체 당 계산한 총 페놀화합물의 함량과 총 항산화도는 RGB 처리구에서 대체로 높은 경향을 보였다(도 4 참조). In addition, the total phenolic compound concentration and antioxidant activity were the highest in fluorescent and RGB 5: 1: 4, but the total phenolic compound content and total antioxidant activity per plant were generally higher in the RGB treatment (see FIG. 4) .

또한, 이고들빼기의 주요 생리활성물질 중 타겟 물질인 치커리산(chicoric acid)의 함량은 생장도 좋았던 RGB 6:1:3 처리구에서 가장 증가하였다. 또한, 주요 생리활성물질인 치커리산(chicoric acid), 카프타릭산(caftaric acid), 클로로제닉산(chlorogenic acid) 및 3,5-DCQA(3,5-di-O-caffeoylquinic acid)의 총 함량도 RGB 6:1:3 처리구에서 가장 높았다(도 5 참조). In addition, the content of chicoric acid, which is a target substance, was the highest in the RGB 6: 1: 3 treatment which was also good growth. The total content of chicoric acid, caftaric acid, chlorogenic acid and 3,5-di-O-caffeoylquinic acid (3,5-DCQA) And the highest in the RGB 6: 1: 3 treatment (see FIG. 5).

따라서, 다양한 LED 광질 처리에서 이고들빼기의 생장과 타겟물질인 치커리산(chicoric acid)의 함량은 RGB 6:1:3 혼합광에서 증대되었고, 이러한 기술은 밀폐형 식물 생산 시스템인 식물공장에서 의약 기반 식물 원료를 균일하고 안정적으로 대량생산이 가능할 것으로 의미한다. Therefore, in the various LED light quality treatments, the growth of the extract and the content of chicoric acid, which is the target substance, were increased in the RGB 6: 1: 3 mixed light, It means that the plant raw material can be mass-produced uniformly and stably.

<< 실시예Example 2>  2> 근적외선광Near-infrared light LED에 따른 Depending on the LED 이고들빼기의And subtract 생장 확인 Growth Confirmation

다양한 근적외선광 LED를 이용하여 이고들빼기의 생장 및 유용 물질을 확인하였다. 식물체의 파이토크롬은 적색광(R)에 의해 불활성 상태에서 활성 상태, 근적외선광(FR)에 의해 활성 상태에서 불활성 상태로 전환된다. 이러한 파이토크롬의 전환은 근적외선광에 대한 적색광의 비율(R/FR)에 따라 식물의 생육과 형태학적인 변화를 발생시킨다. 따라서 본 발명은 RB 8:2 혼합광 LED의 동일한 광도(PPFD) 130μmol/m2/s에서 far-red LED를 추가하여 4가지의 R/FR 비율을 조절하고 추가하지 않은 RB 8:2 LED 혼합광에서 온도 20℃, 이산화탄소 농도 1000ppm, 상대습도 60% 환경의 밀폐형 식물 생산 시스템에서 6주간 재배하였다. Various near-infrared light LEDs were used to identify the growth and useful substances of the extracts. Phytochrome in the plant is converted from the active state to the inactive state by the red light (R) in an inactive state and by the near-infrared light (FR). This conversion of phytochrome causes the growth and morphological changes of the plant depending on the ratio of red light to near-infrared light (R / FR). Accordingly, the present invention can be used to control four R / FR ratios by adding a far-red LED at 130 μmol / m 2 / s of the same light intensity (PPFD) of RB 8: The plant was cultivated for 6 weeks in a closed plant production system with a temperature of 20 ℃ in light, 1000 ppm of carbon dioxide concentration and 60% relative humidity.

Far-Red 처리 후 6주차에 각 광원 별 지상부의 생체중과 건물중, 엽면적, 엽장, 엽폭 및 엽수를 측정하였다. 또한 총 페놀 화합물과 치커리산(chicoric acid), 클로로제닉산(chlorogenic acid) 및 카페익산(caffeic acid)의 농도와 함량, 항산화 능력을 분석하였다. At the 6th week after Far-Red treatment, the fresh weight, the leaf area, the leaf area, the leaf length, the leaf width and the leaf number of the upper part of each light source were measured. The concentration, content and antioxidant capacity of total phenolic compounds, chicoric acid, chlorogenic acid and caffeic acid were also analyzed.

그 결과, 지상부의 생체중과 건물중, 엽면적, 엽장, 엽폭은 FR이 포함되지 않은 대조구와 상업적으로 사용되는 광원(IS)에서 보다 R/FR 비율이 낮은, 즉 FR LED의 비율이 높았던 0.7과 1.2 처리구에서 유의적으로 높았다. 특히, 생육이 가장 좋았던 R/FR 1.2 비율의 처리구에서 대조구와 비교하여 엽수에서는 유의적인 차이를 나타내지 않았으나, 생체중은 2.4배, 건물중은 1.9배 증가하였고 엽장과 엽폭이 크게 증가하여 이에 따른 엽면적 또한 증가하였다(도 7 참조). As a result, the live weight, the leaf area, the leaf area, the leaf area and the leaf width of the above ground were 0.7 and 1.2, which were lower in the R / FR ratio than in the FR Respectively. In particular, the R / FR 1.2 ratio, which was the best growth, did not show a significant difference in the number of leaves compared to the control, but the fresh weight increased 2.4 times and the dry weight increased 1.9 times. The leaf area and leaf width increased significantly, (See FIG. 7).

또한, 이고들빼기 지상부의 단위 건물중 당 총 페놀 농도는 모든 처리구에서 유의적인 차이를 나타내지 않았기 때문에 지상부의 생육이 월등히 좋았던 R/FR 0.7과 1.2 처리구에서의 총 페놀 함량이 가장 높게 나타났고 항산화 능력 또한 유사한 경향을 보였다(도 8 참조). The total phenol concentration in the digestion of the soil was not significantly different in all treatments. Therefore, the total phenol content of R / FR 0.7 and 1.2 treatments, Showing a similar tendency (see FIG. 8).

또한, 주요 생리 활성 물질인 치커리산(chicoric acid), 클로로제닉산(chlorogenic acid) 및 카페익산(caffeic acid)의 농도는 대조구와 비교하여 R/FR 비율이 낮은 0.7과 1.2에서 약간 감소하는 경향을 보였으나 이고들빼기 하나의 식물체에서 생산할 수 있는 함량은 0.7과 1.2에서 높게 나타났다(도 9 참조). The concentrations of chicoric acid, chlorogenic acid and caffeic acid, which are major physiologically active substances, tended to decrease slightly at 0.7 and 1.2, respectively, when the R / FR ratio was lower than that of the control The content that could be produced in one plant was 0.7 and 1.2, respectively (see FIG. 9).

따라서, 본 발명은 약용작물인 이고들빼기의 생육을 증가시키고 기능성 물질 함량을 증대 시키는 적정 R/FR 비율을 규명하여 밀폐형 식물 생산 시스템에서 고품질의 이고들빼기 생산량을 증대시킬 것이다. Thus, the present invention will identify high R and FR yields in closed plant production systems that are medicinal crops and identify the appropriate R / FR ratios that increase the growth of extracts and increase the functional material content.

이제까지 본 발명에 대하여 그 바람직한 실시예들을 중심으로 살펴보았다. 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 특허청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다.The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (7)

LED(Light Emitting Diode) 광을 이용한 이고들빼기(Crepidiastrum denticulatum)의 생장 및 생리활성 물질 증진 방법.Growth of Crepidiastrum denticulatum by using LED (Light Emitting Diode) light and a method for promoting bioactive substances. 제 1항에 있어서, 상기 LED 광은 White(W), Red(R), Green(G) 또는 Blue(B)이고, 상기 LED 광은 R:B 6:4, R:B 7:3, R:B 8:2, R:B 9:1, RGB 5:1:4, RGB 6:1:3, RGB 7:1:2, RGB 8:1:1, RGB 9:1:0, RWB 7:1:2, RWB 8:1:1, RWB 6:2:2, RWB 7:2:1 및 RWB 8:2:0으로 이루어진 군에서 선택된 1종 이상의 혼합 LED 광인 것을 특징으로 하는, LED 광을 이용한 이고들빼기의 생장 및 생리활성 물질 증진 방법.The LED light source according to claim 1, wherein the LED light is at least one of red (R), red (R), green (G) RGB 8: 1: 1, RGB 9: 1: 0, RWB 7: 1: 2, RGB 7: 1: Wherein the LED light is at least one mixed LED light selected from the group consisting of: 1: 2, RWB 8: 1: 1, RWB 6: 2: 2, RWB 7: 2: And a method for promoting growth of physiological active substances. 제 1항에 있어서, 근적외선(far red light)을 추가적으로 조사하는 것을 특징으로 하는 LED 광을 이용한 이고들빼기의 생장 및 생리활성 물질 증진 방법.The method according to claim 1, further comprising the step of irradiating far-infrared light with the LED light. 제 3항에 있어서, 상기 근적외선에 대한 적생광에 대한 비율(R/FR)은 0.7, 1.2, 4.1 및 8.6으로 이루어진 군에서 선택된 1종 이상인 것을 특징으로 하는 LED 광을 이용한 이고들빼기의 생장 및 생리활성 물질 증진 방법.4. The method according to claim 3, wherein the ratio (R / FR) of the near-infrared light to the red light is at least one selected from the group consisting of 0.7, 1.2, 4.1 and 8.6. A method for promoting a physiologically active substance. 제 1항에 있어서, 상기 생장은 생체중, 건물중, 엽수, 엽면적, 엽장 및 엽폭으로 이루어진 군에서 선택된 1종 이상이 증가하는 것을 특징으로 하는 LED 광을 이용한 이고들빼기의 생장 및 생리활성 물질 증진 방법. The method according to claim 1, wherein the growth is increased by at least one selected from the group consisting of live weight, building weight, leaf number, leaf area, leaf length and leaf width. Way. 제 1항에 있어서, 상기 생리활성 물질은 페놀 화합물, 치커리산(chicoric acid), 카프타릭산(caftaric acid), 클로로제닉산(chlorogenic acid), 3,5-DCQA(3,5-O-dicaffeoyl-epi-quinic acid) 및 카페익산(caffeic acid)으로 이루어진 군에서 선택된 1종 이상의 물질이 증가하는 것을 특징으로 하는 LED 광을 이용한 이고들빼기의 생장 및 생리활성 물질 증진 방법.The method of claim 1, wherein the physiologically active substance is selected from the group consisting of phenolic compounds, chicoric acid, caftaric acid, chlorogenic acid, 3,5-DCQA (3,5-O-dicaffeoyl- epi-quinic acid, and caffeic acid. The method of claim 1, wherein the at least one substance selected from the group consisting of epi-quinic acid and caffeic acid is increased. 제 1항의 방법을 이용하여 생장 및 생리활성 물질이 증진된 이고들빼기.Use of the method of claim 1 to enhance and remove the growth and bioactive material.
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