KR20170121927A - Multi-purposed apparatus of treating biomass for producing biomass-derived useful products and method for producing useful products using the same - Google Patents
Multi-purposed apparatus of treating biomass for producing biomass-derived useful products and method for producing useful products using the same Download PDFInfo
- Publication number
- KR20170121927A KR20170121927A KR1020160050803A KR20160050803A KR20170121927A KR 20170121927 A KR20170121927 A KR 20170121927A KR 1020160050803 A KR1020160050803 A KR 1020160050803A KR 20160050803 A KR20160050803 A KR 20160050803A KR 20170121927 A KR20170121927 A KR 20170121927A
- Authority
- KR
- South Korea
- Prior art keywords
- reactor
- biomass
- solvent
- pretreatment
- heat exchanger
- Prior art date
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/02—Apparatus for enzymology or microbiology with agitation means; with heat exchange means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/26—Inoculator or sampler
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/12—Bioreactors or fermenters specially adapted for specific uses for producing fuels or solvents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/20—Heating; Cooling
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Processing Of Solid Wastes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
TECHNICAL FIELD The present invention relates to a technique for treating biomass to obtain various useful products, and more specifically, to a biomass treatment method for treating biomass introduced in a single reactor to produce biomass-derived useful products including biomass pretreatment products, glycosylated products and bioethanol To a multipurpose biomass treatment apparatus for producing useful products derived from biomass and a method for producing useful products using the biomass treatment apparatus.
A number of techniques for biomass pretreatment have been studied for the purpose of producing a substrate that can be hydrolyzed quickly and efficiently to form fermentable sugars from biomass. Generally, the conventional biomass pretreatment processes include steam explosion, alkali treatment, sulfur dioxide treatment, hydrogen peroxide treatment, supercritical ammonia treatment, weak acid treatment, and ammonia freeze-thawing. Among these pretreatment methods, weak acid pretreatment methods and steam explosion pretreatment methods have been extensively studied and are now considered as excellent pretreatment methods. Both processes can effectively dissolve hemicellulose, but it is somewhat ineffective for lignin removal.
On the other hand, there are many problems in the enzymatic hydrolysis of the pretreated biomass. That is, the conditions necessary for the pretreatment of biomass are different from the optimal conditions for enzymatic hydrolysis. The enzymatic hydrolysis is usually carried out at a pH of about 4 to 6.5 and a temperature condition of about 30 to 55 ° C, but the biomass pretreatment method is generally too acidic and too hot compared to enzymatic hydrolysis conditions. The yeast-based enzyme reaction is typically carried out at a temperature of, for example, about 28 ° C to 40 ° C, and the thermophilic bacterium-based enzyme hydrolysis can proceed at a temperature as high as about 80 ° C and is based on mesophilic bacteria Enzymatic hydrolysis occurs at a temperature of about 20 째 C to about 45 째 C.
Moreover, conventional reactors used for continuous hydrolysis of the enzymes (in which there is an injection stream and a discharge stream and the reaction vessel is kept constant) require a large tank with an expensive and powerful impeller to mix the enzyme with the slurry , Enzymatic hydrolysis or liquefaction of biomass requires tens of hours (typically more than 24 hours) when mixed in large tanks.
In addition, the pretreated biomass must be cooled to a temperature suitable for enzymatic hydrolysis. For example, the pretreated biomass may be removed from the high temperature (e.g., 100 占 폚) to substantially the cooling system temperature (e.g., 20 占 폚 to 65 占 폚) during discharge of the pretreatment vessel prior to entering the reaction vessel for enzymatic hydrolysis Lt; 0 > C).
Due to these various problems, it is almost impossible to obtain a useful product with high efficiency by glycation and fermentation successively after pretreatment of biomass according to the conventional biomass treatment devices.
The inventors of the present invention have completed the present invention by developing a technique for obtaining a pre-treatment biomass by pretreatment of a biomass, followed by saccharification and fermentation sequentially to obtain useful products with higher efficiency.
Accordingly, an object of the present invention is to provide a biomass treatment method capable of mass-producing biomass by mass-producing a useful product by saccharification and fermentation in the same reactor in which pretreatment has been performed without transferring pretreated biomass obtained by pretreating biomass to a saccharification tank or the like In addition, it is an object of the present invention to provide a multi-purpose biomass treatment apparatus with a novel structure capable of obtaining useful products obtained from biomass with high efficiency.
Another object of the present invention is to provide a multipurpose biomass treatment method capable of obtaining at least one of pre-treated biomass, glycosylated product, and fermented product, which are useful products obtained by treating biomass through a multipurpose biomass treatment apparatus .
The object of the present invention is not limited to the above-mentioned objects, and although not explicitly mentioned, the object of the invention which can be recognized by a person skilled in the art from the description of the detailed description of the invention .
In order to accomplish the objects of the present invention, first, the present invention provides a reactor comprising: a reactor in which biomass and a solvent are charged and processed; A heat exchanger connected to the reactor and the first connecting member to heat or cool the solvent introduced into the reactor to provide the heated or cooled solvent to the reactor; A circulator which is installed between the reactor and the heat exchanger and circulates the solvent heated or cooled by the heat exchanger along the first connection member at a heat exchange knob with the reactor; And a cooler connected to the reactor and the second connecting member to collect the gaseous solvent vaporized in the reactor and to liquefy the collected solvent to provide the reactor to the reactor.
In a preferred embodiment, the reactor comprises a body defining a closed rectangular interior space; A lid provided on an upper end or a side wall of the main body to open and close the inner space; A plurality of discharge ports formed on a lower portion or a side wall of the main body including a first discharge port connected to the first connection member and a second discharge port connected to the second connection member; And a plurality of injection ports formed on an upper portion or a side wall of the main body including a first injection port connected to the first connection member and a second injection port connected to the second connection member.
In a preferred embodiment, the main body further includes an injury preventing means installed on the inner space so that the biomass floating on the solvent is immersed in the solvent.
In a preferred embodiment, the float prevention means includes a plurality of fixing members protruding in parallel with the ground on the side wall of the main body and formed at a predetermined interval; And a porous member fixed to cover the horizontal section of the inner space detachably to the fixing member.
In a preferred embodiment, the flotage prevention means comprises a flotage prevention member having the same shape as the horizontal cross-section of the inner space, the flotation prevention member having a size and a porosity capable of moving up and down in the inner space; And a height adjusting member whose one end is fixed to the central portion of the upper surface of the flotation preventing member and whose other end is fixed to the center side of the body upper end portion and is adjustable in the vertical direction in parallel with the side wall.
In a preferred embodiment, the apparatus further comprises a filtering member for filtering the solvent discharged from the reactor to the first connecting member through the first outlet.
In a preferred embodiment, the first connecting member includes a first tubular member connecting a first outlet for discharging the solvent to the outside of the reactor and the heat exchanger, and a solvent having passed through the heat exchanger and the heat exchanger are injected into the reactor And a second tubular member connecting the first injection port.
In a preferred embodiment, the first tubular member is further provided with a first opening / closing member which is installed in proximity to a portion connected to the heat exchanger and can open / close the first tubular member.
In a preferred embodiment of the present invention, at least one of the first tubular members is provided at a front end of a portion where the first opening and closing member is provided, and at least one discharge member discharging the solvent through the first tubular member to the outside.
In a preferred embodiment, the discharge member comprises a first discharge member for discharging the saccharification product or the fermentation product from the solvent passing through the first tubular member, a second discharge member for discharging the pre-treatment solvent out of the solvent passing through the first tubular member, A discharge member, and a third discharge member for discharging the wash water in the solvent passing through the first tubular member.
In a preferred embodiment, the first discharge member is connected to a final product recovery tank for obtaining the saccharified product or the fermented product, and the second discharge member is connected to a pretreatment solvent tank for storing the pretreatment solvent, 3 discharge member is connected to a waste tank or a purifier for treating the wash water.
In a preferred embodiment, the apparatus further comprises pumping means for retransmitting the pretreatment solvent from the pretreatment solvent tank to the heat exchanger through the first tubular member.
In a preferred embodiment, the discharge member is a tubular member connected to the first tubular member and provided on one end side adjacent to the first tubular member, and includes an opening / closing control valve for regulating opening / closing with the first tubular member .
In a preferred embodiment, the apparatus further comprises a wash water storage tank or a water pipe connected to the inlet of the reactor to supply wash water into the reactor.
In a preferred embodiment, the apparatus further comprises an enzyme storage tank connected to the inlet of the reactor to supply the enzyme into the reactor, and a buffer storage tank for supplying the buffer.
In a preferred embodiment, the apparatus further comprises a yeast storage tank connected to the inlet of the reactor to supply yeast into the reactor.
In a preferred embodiment, a third tubular member connecting the second outlet to the outside with the cooler, and a fourth tubular member connecting the cooler and the second injection port into which the solvent cooled by the cooler is injected, .
In a preferred embodiment, a flow rate control means for controlling the flow of the solvent discharged or introduced through the discharge port or the injection port formed in the reactor is further provided outside the reactor adjacent to the discharge port or the injection port.
In a preferred embodiment, when the biomass treatment conditions including the reaction temperature, the solvent amount, and the reaction time are set according to the biomass introduced into the reactor, the reactor, the heat exchanger, the circulator, the cooler, And a controller for controlling each component including the means for processing the biomass.
In a preferred embodiment, the biomass introduced into the reactor is treated to obtain at least one of a pretreatment biomass, a biomass saccharification product and a biomass fermentation product.
The present invention also provides a process for producing a biodegradable polymer, comprising: introducing a biomass and a pretreatment solvent into a reactor; Transporting the pretreatment solvent to a heat exchanger along a first connecting member connecting the reactor and the heat exchanger and raising the temperature inside the reactor to a reaction temperature through a circulation process of re-transporting the pretreated solvent heated in the heat exchanger to the reactor ; A pretreatment step of pre-treating the biomass with a pretreatment solvent at the reaction temperature; Removing the pretreatment solvent through the first outlet of the reactor and recovering the pretreatment solvent to the pretreatment solvent storage tank when the pretreatment reaction is completed; And washing or recovering the pretreated biomass obtained in the reactor.
In a preferred embodiment, the step of washing the pretreatment biomass obtained in the reactor comprises the steps of: supplying wash water to the reactor from which the pretreatment solvent has been discharged; Circulating the supplied washing water between the reactor and the heat exchanger to wash the pretreated biomass; And discharging the washing water from the reactor when the washing of the pretreatment biomass is completed, and transporting the washing water to a waste tank or a purification tank.
In a preferred embodiment, the method comprises the steps of: supplying an enzyme and a buffer to a reactor in which the washed pretreatment biomass is present; The enzyme and the buffer solution are transported to the heat exchanger along the first linking member and the enzyme and buffer heated in the heat exchanger are transported to the reactor again to increase the temperature inside the reactor to the reaction temperature, Glycation reaction of the pretreatment biomass; And discharging the saccharified product from the reactor when the saccharification reaction is completed, thereby obtaining a biomass saccharified product into a final product recovery tank.
In a preferred embodiment, the method comprises the steps of: supplying the enzyme, buffer and yeast to a reactor in which the washed pretreatment biomass is present; The temperature of the inside of the reactor is raised to the reaction temperature by circulating the enzyme, the buffer and the yeast mixed solution through the first connecting member to the heat exchanger, and circulating the enzyme, buffer and yeast mixture heated in the heat exchanger to the reactor again And simultaneously subjecting the pretreated biomass to the simultaneous saccharification fermentation reaction while maintaining the reaction temperature; And discharging the biomass fermentation product from the reactor when the simultaneous saccharification fermentation reaction is completed, thereby obtaining a biomass fermentation product as a final product recovery tank.
According to the above-described multipurpose biomass treatment apparatus of the present invention, the pretreated biomass obtained by pretreating the biomass can be saccharified and fermented in the same reactor in which the pretreatment has been carried out without moving the biomass separately to the saccharification tank or the like, Not only biomass treatment is possible but also useful products obtained from the biomass can be obtained with high efficiency.
According to the multi-purpose biomass processing method of the present invention, at least one of the pre-processed biomass, glycation product and fermentation product, which are available products obtained by processing the biomass through a multi-purpose biomass processing apparatus of a new structure, Can be obtained.
These technical advantages of the present invention are not limited to the above-mentioned technical scope, and even if not explicitly mentioned, the effect of the invention which can be recognized by a person skilled in the art from the description of the concrete contents for carrying out the invention Of course.
1 is a schematic view of a multipurpose biomass processing apparatus for biomass processing according to an embodiment of the present invention.
2A and 2B are specific examples of the reactor of the multipurpose biomass processing apparatus shown in FIG.
FIG. 3 is a flowchart showing a process for obtaining a biomass saccharified product or a biomass fermentation product from a biomass as a final product in the multipurpose biomass processing apparatus shown in FIG. 1;
FIG. 4 is a graph showing a process in which the temperature of the reactor is gradually increased by operating the heat exchanger during the pretreatment reaction in the multi-purpose biomass treatment apparatus shown in FIG. 1, and then gradually cooled.
FIG. 5 is a graph of results obtained in the pretreatment as in Example 1 using the multipurpose biomass treatment apparatus shown in FIG.
While the present invention has been described in connection with what is presently considered to be the most practical and preferred embodiment, it is to be understood that the invention is not limited to the disclosed embodiments. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Accordingly, the terms used in the present invention should not be construed to be mere terms, but should be interpreted based on the ordinary meanings of the terms and contents described throughout the specification of the present invention. In particular, where the use of the terms " about ", "substantially" or " about " is used, it can be interpreted that the manufacturing and material tolerances inherent in the stated meanings are used in the numerical value or in close proximity to the numerical value .
Hereinafter, the technical structure of the present invention will be described in detail with reference to the accompanying drawings and preferred embodiments.
However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Like reference numerals used to describe the present invention throughout the specification denote like elements.
The technical feature of the present invention is that the pretreated biomass obtained by pretreating the biomass is not separately transported to a saccharification tank and the saccharification and fermentation process is performed in the same reactor in which the pretreatment has been carried out to produce fermentation products including glycosylated products and bioethanol The present invention relates to a multipurpose biomass treatment apparatus and a multipurpose biomass treatment method having a structure capable of mass production of biomass-derived useful products.
Accordingly, the multipurpose biomass treatment apparatus of the present invention comprises a reactor in which biomass and a solvent are introduced and processed; A heat exchanger connected to the reactor and the first connecting member to heat or cool the solvent introduced into the reactor to provide the heated or cooled solvent to the reactor; A circulator which is installed between the reactor and the heat exchanger and circulates the solvent heated or cooled by the heat exchanger along the first connection member at a heat exchange knob with the reactor; And a cooler connected to the reactor and the second connecting member to collect the gaseous solvent vaporized in the reactor and liquefy the collected solvent to the reactor.
In addition, the method for treating a multipurpose biomass of the present invention comprises the steps of injecting a biomass and a pretreatment solvent into a reactor; Transporting the pretreatment solvent to a heat exchanger along a first connecting member connecting the reactor and the heat exchanger and raising the temperature inside the reactor to a reaction temperature through a circulation process of re-transporting the pretreated solvent heated in the heat exchanger to the reactor ; A pretreatment step of pre-treating the biomass with a pretreatment solvent at the reaction temperature; Removing the pretreatment solvent through the first outlet of the reactor and recovering the pretreatment solvent to the pretreatment solvent storage tank when the pretreatment reaction is completed; And washing or recovering the pretreatment biomass obtained in the reactor.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS A detailed description will be given of specific embodiments according to the present invention with reference to the drawings.
FIG. 1 is a schematic view of a multipurpose biomass processing apparatus for biomass processing according to one aspect of the present invention. FIGS. 2a and 2b are views showing a concrete implementation of a reactor of the multipurpose biomass processing apparatus shown in FIG. FIG. 3 is a flow chart showing the process of obtaining a biomass saccharified product or a biomass fermentation product as a final product from the biomass in the multipurpose biomass processing apparatus shown in FIG. 1, and FIG. 4 is a flowchart showing the multipurpose biomass processing apparatus shown in FIG. It is a measurement graph showing the process in which the heat exchanger is operated during the pretreatment reaction in the biomass treatment device to raise the temperature of the reactor and then gradually cool down. FIG. 5 is a graph of results obtained in the pretreatment as in Example 1 using the multipurpose biomass treatment apparatus shown in FIG.
Referring to FIG. 1, the multipurpose
The
The
If necessary, the
As another embodiment of the injury preventing means, an injury preventing means including the
The
The outlet is a component that performs a function of discharging various reactants obtained in the course of performing the pretreatment, saccharification and fermentation of the biomass in the
The injection port is a component that performs a function of injecting a substance necessary for performing the pretreatment, saccharification and fermentation of biomass into the
The
The
The
The cooler 140 is connected to the
The
At this time, the first
The
The
As shown in FIG. 1, the
The apparatus may further include a pumping means for retransmitting the pretreated solvent recovered and stored from the pretreatment
The second connecting
If the biomass treatment conditions including the reaction temperature, the solvent amount, and the reaction time are set according to the type and content of the biomass to be fed into the
A multipurpose biomass treatment method capable of obtaining a pretreatment biomass, a biomass glycosylation product, and a biomass fermentation product from biomass using the multipurpose biomass treatment apparatus having the above-described configuration will be described.
As shown in FIG. 3, the biomass and the pretreatment solvent are introduced into the reactor 110 (S1). The feed of biomass and pretreatment solvent into the
The pretreating solvent introduced into the
When the inside of the
After the pre-treatment reaction is completed, the first connecting member is closed through the first opening / closing member (omitted in FIG. 152) provided in the first connecting
When the pretreatment solvent is recovered, only the pretreatment biomass remains in the
When washing of the pretreatment biomass is completed, a saccharification reaction for the pretreated biomass washed in the
In some cases, when the washing of the pretreatment biomass is completed, the simultaneous saccharification fermentation reaction to the pretreated biomass washed in the
Example 1
20 kg of oak wood chips and 80.9 kg of pre-treatment solvent were charged into the reactor of the multipurpose biomass treatment apparatus and the pre-treatment reaction was performed within 2 hours as shown in FIG. 5 to obtain 43.0 kg (water content 71.0%) of the pretreated biomass 1, . At this time, the recovery rate of biomass was 62.4%.
Example 2
20 kg of pine chips as a biomass and 80.9 kg of a pretreatment solvent were added to a reactor of a multipurpose biomass treatment apparatus and subjected to a pretreatment reaction for 2 hours and 30 minutes to obtain 53.1 kg (water content 74.1%) of
Example 3
20 kg of rice straw and 80.9 kg of pretreatment solvent were added to the reactor of the multipurpose biomass treatment apparatus and pretreated for 2 hours and 30 minutes to obtain 65.3 kg of pre-treatment biomass 3 (water content 76.6%). At this time, the recovery rate of biomass was 76.4%.
Experimental Example 1
The chemical components of the pretreatment biomass 1 to 3 obtained in Examples 1 to 3 were analyzed and the results are shown in Table 1.
Table 1 shows that the lignin content in the pretreatment biomass obtained after pretreatment of the herbaceous system biomass as well as the tree system was reduced in the biomass of the present invention, .
Example 4
After the pretreatment biomass 1 obtained in Example 1 was washed, an enzyme and a buffer were added to the reactor, and the saccharification process was performed to obtain the enzyme hydrolyzate 1. At this time, the enzyme input was treated with 10 FPU / g biomass of cellulase and 200 IU / g biomass of xylanase.
Example 5
The enzyme hydrolyzed
Example 6
The same procedure as in Example 4 was carried out except that the pretreatment biomass 3 obtained in Example 3 was used to obtain an enzyme hydrolyzed glycoside 3.
Experimental Example 2
The contents of the biosugars contained in the enzymatic hydrolysates 1 to 3 obtained in Examples 4 to 6 were analyzed, and the results are shown in Table 2.
Example 7
After washing the pretreatment biomass 1 obtained in Example 1, the enzyme, the buffer and the yeast were added to the reactor, and the simultaneous saccharification step was performed to obtain the co-saccharified fermentation broth 1. At this time, the enzyme input was treated with 10 FPU / g biomass of cellulase and 200 IU / g biomass of xylanase, and 10% of total yeast saccharification fermentation broth was added to the yeast culture.
Example 8
The simultaneous
Example 9
A simultaneous saccharified fermentation broth 3 was obtained in the same manner as in Example 7 except that the pretreatment biomass 3 obtained in Example 3 was used.
Experimental Example 3
The content of bioethanol obtained in the simultaneous saccharified fermentation broths 1 to 3 obtained in Examples 7 to 9 was analyzed and the results are shown in Table 3. [
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation, Various changes and modifications will be possible.
100: Multipurpose biomass processing unit
110: Reactor 111: Body
112:
113b:
114b: second inlet port 115: filtration member
116: fixing member 117: porous member
118: height adjustment member
120: heat exchanger 130: circulator
140: cooler 150: first connecting member
151: first tubular member 152: first opening / closing member
153: discharge member 153a: connection pipe
153b: opening /
153d: 2
154: pumping means 155: second tubular member
160: second connecting member 161: third tubular member
162: fourth tubular member 170: flow rate adjusting means
191: Final product yield tank
192: Pretreatment solvent tank 193: Waste tank
194: Washing water storage tank 195: Enzyme storage tank
196: Buffer storage tank 197: Yeast storage tank
Claims (24)
A heat exchanger connected to the reactor and the first connecting member to heat or cool the solvent introduced into the reactor to provide the heated or cooled solvent to the reactor;
A circulator which is installed between the reactor and the heat exchanger and circulates the solvent heated or cooled by the heat exchanger along the first connection member at a heat exchange knob with the reactor; And
And a cooler connected to the reactor and the second connecting member to collect gaseous solvent vaporized in the reactor and liquefy the collected solvent to the reactor.
The reactor comprises a body defining a closed rectangular interior space; A lid provided on an upper end or a side wall of the main body to open and close the inner space; A plurality of discharge ports formed on a lower portion or a side wall of the main body including a first discharge port connected to the first connection member and a second discharge port connected to the second connection member; And a plurality of injection ports formed on an upper portion or a side wall of the main body, the main injection port including a first injection port connected to the first linking member and a second injection port connected to the second linking member Device.
Wherein the main body further comprises injury preventing means installed on the inner space so that the biomass floating on the solvent is immersed in the solvent.
Wherein the flotation preventing means comprises a plurality of fixing members protruding in parallel to the paper surface on the side wall of the main body and formed at a predetermined interval; And a porous member fixed to cover the horizontal section of the inner space in a detachable manner to the fixing member.
Wherein the flotage prevention means comprises: a flotage prevention member having the same shape as the horizontal cross-section of the inner space, the flotation prevention member having a size and a porosity that allows a vertical movement in the inner space; And a height adjusting member fixed at a center portion of the upper surface of the flotage preventing member and the other end fixed to the center of the upper end of the body and being adjustable in the vertical direction in parallel with the side wall Wherein the biomass treatment device is a multi-purpose biomass treatment device.
Further comprising a filtration member for filtering the solvent discharged from the reactor to the first connection member through the first discharge port.
The first connection member connects a first tubular member connecting the heat exchanger with a first discharge port for discharging the solvent to the outside of the reactor, and a first injection port through which the solvent passed through the heat exchanger and the heat exchanger is injected into the reactor Wherein the first tubular member comprises a second tubular member.
Further comprising a first opening / closing member installed adjacent to a portion of the first tubular member connected to the heat exchanger and capable of opening and closing the first tubular member.
Further comprising at least one discharge member provided at a front end of a portion of the first tubular member where the first openable and closable member is provided to discharge the solvent passing through the first tubular member to the outside, Device.
Wherein the discharging member comprises a first discharging member for discharging the saccharified product or the fermentation product in the solvent passing through the first tubular member, a second discharging member for discharging the pretreatment solvent in the solvent passing through the first tubular member, And a third discharge member for discharging the washing water in the solvent passing through the one tubular member.
Wherein the first discharge member is connected to a final product recovery tank for obtaining the saccharified product or fermented product and the second discharge member is connected to a pretreatment solvent tank for storing the pretreatment solvent, To a waste tank or a purification device for treating the biomass.
Further comprising a pumping means for retransferring the pretreatment solvent from the pretreatment solvent tank to the heat exchanger through the first tubular member.
Wherein the discharge member is a tubular member connected to the first tubular member and provided at one end side adjacent to the first tubular member for controlling opening and closing with the first tubular member. Mass processing device.
And a washing water storage tank or a water pipe connected to an inlet of the reactor to supply washing water into the reactor.
An enzyme storage tank connected to an inlet of the reactor to supply an enzyme into the reactor, and a buffer storage tank for supplying a buffer solution.
Further comprising a yeast storage tank connected to an inlet of the reactor to supply yeast into the reactor. ≪ Desc / Clms Page number 19 >
Wherein the second connecting member comprises a third tubular member connecting a second outlet for discharging the solvent vaporized in the reactor to the outside of the reactor and a third tubular member connecting the cooler and a second tubular member connected to the second tubular member for discharging the solvent cooled in the cooler, And a fourth tubular member connecting the injection port.
Wherein the flow rate regulating means for controlling the flow of the solvent discharged or introduced through the discharge port or the injection port formed in the reactor is further provided outside the reactor adjacent to the discharge port or the injection port.
When the biomass treatment conditions including the reaction temperature, the solvent amount, and the reaction time are set according to the biomass introduced into the reactor, the biomass treatment conditions are set according to the preset conditions, and each component including the reactor, heat exchanger, circulator, cooler, Further comprising a controller for controlling the biomass to control the biomass.
Wherein the biomass introduced into the reactor is treated to obtain at least one of a pretreatment biomass, a biomass glycosylation product, and a biomass fermentation product.
Transporting the pretreatment solvent to a heat exchanger along a first connecting member connecting the reactor and the heat exchanger and raising the temperature inside the reactor to a reaction temperature through a circulation process of re-transporting the pretreated solvent heated in the heat exchanger to the reactor ;
A pretreatment step of pre-treating the biomass with a pretreatment solvent at the reaction temperature;
Removing the pretreatment solvent through the first outlet of the reactor and recovering the pretreatment solvent to the pretreatment solvent storage tank when the pretreatment reaction is completed; And
And washing or recovering the pretreated biomass obtained in the reactor.
The step of washing the pretreatment biomass obtained in the reactor comprises:
Supplying washing water to the reactor in which all of the pretreatment solvent has been discharged;
Circulating the supplied washing water between the reactor and the heat exchanger to wash the pretreated biomass; And
And discharging the washing water from the reactor when the washing of the pretreatment biomass is completed, and transporting the washing water to a waste tank or a purification tank.
Supplying an enzyme and a buffer to a reactor in which the washed pretreatment biomass is present;
The enzyme and the buffer solution are transported to the heat exchanger along the first linking member and the enzyme and the buffer heated in the heat exchanger are re-transported to the reactor, the temperature inside the reactor is raised to the reaction temperature, And allowing the pre-treatment biomass to be saccharified; And
And discharging the biomass saccharified product from the reactor after completion of the saccharification reaction to obtain a biomass saccharified product as a final product recovery tank.
Supplying an enzyme, a buffer and a yeast to a reactor in which the washed pretreatment biomass is present;
The temperature of the inside of the reactor is raised to the reaction temperature by circulating the enzyme, the buffer and the yeast mixed solution through the first connecting member to the heat exchanger, and circulating the mixed enzyme, buffer and yeast mixture heated in the heat exchanger to the reactor And simultaneously subjecting the pretreated biomass to the simultaneous saccharification fermentation reaction while maintaining the reaction temperature; And
And discharging the biomass fermentation product from the reactor after completion of the simultaneous saccharification fermentation reaction to obtain a biomass fermentation product as a final product recovery tank.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020160050803A KR101888078B1 (en) | 2016-04-26 | 2016-04-26 | Multi-purposed apparatus of treating biomass for producing biomass-derived useful products and method for producing useful products using the same |
PCT/KR2017/003342 WO2017188611A1 (en) | 2016-04-26 | 2017-03-28 | Multi-purpose biomass treatment apparatus for production of biomass-derived useful product and method for production of useful product using same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020160050803A KR101888078B1 (en) | 2016-04-26 | 2016-04-26 | Multi-purposed apparatus of treating biomass for producing biomass-derived useful products and method for producing useful products using the same |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020180089375A Division KR101969059B1 (en) | 2018-07-31 | 2018-07-31 | Multi-purposed apparatus of treating biomass for producing biomass-derived useful products |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20170121927A true KR20170121927A (en) | 2017-11-03 |
KR101888078B1 KR101888078B1 (en) | 2018-08-13 |
Family
ID=60159810
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020160050803A KR101888078B1 (en) | 2016-04-26 | 2016-04-26 | Multi-purposed apparatus of treating biomass for producing biomass-derived useful products and method for producing useful products using the same |
Country Status (2)
Country | Link |
---|---|
KR (1) | KR101888078B1 (en) |
WO (1) | WO2017188611A1 (en) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH1097721A (en) | 1997-09-01 | 1998-04-14 | Olympus Optical Co Ltd | Information reader |
KR20010048482A (en) * | 1999-11-26 | 2001-06-15 | 손재익 | Methods and equipments of ammonia recycled percolation for biomass treatment |
KR100977721B1 (en) * | 2010-01-25 | 2010-08-24 | 주식회사 남보 | Biomass pretreatment method using cyclic lignin extraction reactor system and ammonia |
KR20150041666A (en) | 2012-09-10 | 2015-04-16 | 안드리츠 인코포레이티드 | Method and apparatus for cooling pretreated biomass prior to mixing with enzymes |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101122452B1 (en) * | 2008-07-09 | 2012-02-29 | 지에스칼텍스 주식회사 | Method for manufacturing bio-fuel |
KR101037708B1 (en) * | 2010-04-30 | 2011-05-30 | 전남대학교산학협력단 | Process for the pre-treatment of lignocellulosic biomass by popping method combined with microwave and process for the production of saccarification and bio-ethanol using the same |
KR101554874B1 (en) * | 2013-10-28 | 2015-10-22 | 한국세라믹기술원 | Manufacturing Method Of Saccharides Using Lignocellulose Biomass |
-
2016
- 2016-04-26 KR KR1020160050803A patent/KR101888078B1/en active IP Right Grant
-
2017
- 2017-03-28 WO PCT/KR2017/003342 patent/WO2017188611A1/en active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH1097721A (en) | 1997-09-01 | 1998-04-14 | Olympus Optical Co Ltd | Information reader |
KR20010048482A (en) * | 1999-11-26 | 2001-06-15 | 손재익 | Methods and equipments of ammonia recycled percolation for biomass treatment |
KR100977721B1 (en) * | 2010-01-25 | 2010-08-24 | 주식회사 남보 | Biomass pretreatment method using cyclic lignin extraction reactor system and ammonia |
KR20150041666A (en) | 2012-09-10 | 2015-04-16 | 안드리츠 인코포레이티드 | Method and apparatus for cooling pretreated biomass prior to mixing with enzymes |
Non-Patent Citations (2)
Title |
---|
Biotechnology for Biofuels, Vol. 8. pp. 228(1-11) (2015.12.24.) * |
RSC Adv., Vol. 4, pp. 55318-55327 (2014.) * |
Also Published As
Publication number | Publication date |
---|---|
WO2017188611A1 (en) | 2017-11-02 |
KR101888078B1 (en) | 2018-08-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103627621B (en) | Solid-state vertical type fermentation, self-circulation cooling, distillation integrated apparatus and method | |
US10538447B2 (en) | Optimised energy efficiency hydrothermal carbonization method and device | |
US5525229A (en) | Process and apparatus for anaerobic digestion | |
US9238827B2 (en) | Biomass hydrothermal decomposition apparatus and method | |
US10266422B2 (en) | Method for continuous thermal hydrolysis with recirculation of recovered steam | |
EP3415472B1 (en) | Process and integrated plant for biogas production from organic wastes, nutrients recovery, and purification of produced biogas | |
US20170210625A1 (en) | Hydrothermal carbonization method and device with optimised sludge and steam mixing | |
CN106010662A (en) | Two-step organic waste treatment system and method based on supercritical/subcritical water technology | |
CN1240492C (en) | Method for processing waste products and corresponding processing plant | |
KR20130098082A (en) | Biogas production system using thermal hydrolysis and method for treating organic material using the biogas production system | |
KR101969059B1 (en) | Multi-purposed apparatus of treating biomass for producing biomass-derived useful products | |
BR102013021902A2 (en) | Ethanol and biogas production method, and ethanol installation for ethanol and biogas production | |
CN205603586U (en) | Crop straw enzymolysis tank | |
KR20110079849A (en) | A biomass treatment process and system | |
KR101888078B1 (en) | Multi-purposed apparatus of treating biomass for producing biomass-derived useful products and method for producing useful products using the same | |
CN107021600A (en) | The technique and device of the recovery of biogas slurry ammonia nitrogen and methane purification after pig manure anaerobic fermentation | |
KR101085270B1 (en) | System and method for treating organic waste of menbrane-method using buffer reactor | |
CN110407411A (en) | A kind of livestock and poultry feces anaerobic digestion biogas slurry pyrohydrolysis reclaiming system and method | |
KR101978428B1 (en) | System for energizing organic waste and method thereof | |
KR20180128060A (en) | Methane fermentation device | |
CN107697969A (en) | A kind of system and method for reducing biogas slurry ammonia nitrogen concentration | |
CN206199301U (en) | A kind of batch preparation facilities of hydro-thermal method synthetic catalyst | |
CN112320937A (en) | Efficient anaerobic treatment system and efficient anaerobic treatment method | |
KR100553231B1 (en) | Continuous anaerobic hydrogen-fermenting process for treating organic waste and method of producing hydrogen gas | |
CN205662438U (en) | Biochemical sludge decrement integrated device |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
E902 | Notification of reason for refusal | ||
E902 | Notification of reason for refusal | ||
E701 | Decision to grant or registration of patent right | ||
A107 | Divisional application of patent | ||
GRNT | Written decision to grant |