KR20170109801A

KR20170109801A - Cosmetic composition comprising liposome complex containing gamma polyglutamic acid and gamma oligopeptide

Info

Publication number: KR20170109801A
Application number: KR1020160033847A
Authority: KR
Inventors: 최종완; 김태원; 임미진; 박진우
Original assignee: 주식회사 한국화장품제조
Priority date: 2016-03-22
Filing date: 2016-03-22
Publication date: 2017-10-10
Also published as: KR101845517B1

Abstract

The present invention relates to a cosmetic composition comprising a liposome complex containing polygamma glutamic acid and gamma oligopeptide. The cosmetic composition of the present invention contains a polygamma glutamic acid and has a characteristic of moisturizing effect and a wrinkle-improving effect by containing a gamma-oligopeptide. Also, it is characterized by high content of polygamma glutamic acid and gamma-oligopeptide stably contained through liposomization. Thus, the liposomal complexes have the advantage of moisturizing and wrinkle-reducing effects by adding an appropriate amount to W / O, W / Si or anhydrous formulations. Therefore, the cosmetic composition of the present invention may be applied variously as a cosmetic product.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition comprising a liposome complex containing polygamma glutamic acid and gamma-oligopeptide,

The present invention relates to a cosmetic composition comprising a liposome complex containing polygamma glutamic acid and a gamma oligopeptide. More specifically, the present invention relates to a cosmetic composition comprising a liposome complex containing polygamma glutamic acid and gamma oligopeptide stably without precipitation, And to a cosmetic composition having an improving effect.

Polygamma glutamic acid (Formula 1)

[Chemical Formula 1]

Glutamic acid is an anionic macromolecule peptide linked to gamma-peptide bonds, and is produced by fermentation of Bacillus subtilis chungkookjang, a patented microorganism (Korean Patent Laid-open No. 10-2002-0079889). It also has many potential uses ranging from food to medicines to water treatment. Currently, it is widely used in drug delivery systems in cancer therapy, and studies are underway on its application to type 1 diabetes treatment and its potential use in the production of AIDS vaccines.

On the other hand, polygamma glutamic acid and gamma oligopeptide are raw materials having a moisturizing and wrinkle-improving function, respectively, but they can function as a cosmetic ingredient by containing an appropriate content to exhibit the effect. Since they exist as water-soluble powders, they have difficulty in containing a proper amount especially in W / O formulations, W / Si and anhydrous formulations.

Korean Patent Publication No. 10-2002-0079889

In order to solve the problems of the prior art, it has been found that the effect of improving the moisturizing and wrinkling of w / o, w / Si or waterless form without precipitation and separation of polygamma glutamic acid and gamma oligopeptide And a cosmetic composition containing the same.

In order to solve the above problems, the present invention provides a cosmetic composition comprising a liposome complex containing polygamma glutamic acid and gamma-oligopeptide.

In one embodiment, the content of polygamma glutamic acid and gamma oligopeptide contained in the liposome complex may be 0.01 to 8 wt% based on the total content of the liposome complex.

Another embodiment may comprise 4 to 6% by weight of polygamma glutamic acid, and 2 to 4% by weight of gamma oligopeptide, based on the total content of the liposome complex.

Another embodiment may be that the liposome complex is comprised between 0.1 and 10% by weight based on the total content of the cosmetic composition.

In another embodiment, the cosmetic composition may be for moisturizing or wrinkle-improving.

In another embodiment, the cosmetic composition may be formulated into an oil-in-water (O / W) type, a water-in-oil type (W / O) type, a silicone water-in-water type (W / Si) type and a water-free type.

The present invention also provides a cosmetic comprising the cosmetic composition, wherein the cosmetic is characterized by excellent moisturizing and wrinkle-reducing effects.

The present invention also provides a cosmetic method comprising the step of applying the cosmetic composition to the skin.

The cosmetic composition of the present invention contains a polygamma glutamic acid and has a characteristic of moisturizing effect and a wrinkle-improving effect by containing a gamma-oligopeptide. Also, it is characterized by high content of polygamma glutamic acid and gamma-oligopeptide stably contained through liposomization. Thus, the liposomal complexes have the advantage of moisturizing and wrinkle-reducing effects by adding an appropriate amount to W / O, W / Si or anhydrous formulations. Therefore, the cosmetic composition of the present invention may be applied variously as a cosmetic product.

Fig. 1 shows the results of an evaluation test for the availability and precipitation of poly (gamma glutamic acid) according to the concentration of Test Example 1. Fig.
Fig. 2 shows the results of evaluation of the availability and precipitation of gamma-oligopeptide according to the concentration of Test Example 2. Fig.
FIG. 3 is a graph showing the results of an experiment for evaluating the blending ratio of polygamma glutamic acid and gamma-oligopeptide within the 8% by weight limit, which is the maximum content of the cosmetic composition of Test Example 3 contained in the liposome complex.
Fig. 4 shows the results of the evaluation of the application and separation of liposome complex contents for each formulation according to the cosmetic composition of Test Example 3. Fig.

The present invention relates to a cosmetic composition comprising a liposome complex containing polygamma glutamic acid and a gamma oligopeptide.

The cosmetic composition of the present invention has a moisturizing effect by polygamma glutamic acid and a wrinkle-reducing effect by gamma-oligopeptide, and can be added to W / O, O / W or anhydrous formulations using a liposome complex, It is an advantage that the gamma-oligopeptide does not precipitate and shows moisturizing and wrinkle-reducing effects.

Hereinafter, the present invention will be described in detail.

The present invention provides a cosmetic composition comprising a liposome complex containing polygamma glutamic acid and gamma oligopeptide.

The liposome complex refers to a multi-layered spherical or ellipsoidal structure in which a lipid bilayer structure composed of a lipophilic substance and a hydrophilic substance is continuously formed. In a region where a hydrophilic substance of the liposome complex exists, the polygamma glutamic acid and the gamma oligopeptide .

The polygamma glutamic acid is coated on the skin to prevent moisture from escaping and exhibits a moisturizing effect. It is known that the above polygamma glutamic acid can capture 2.5 to 4 times more moisture even in comparison with hygroscopicity with hyaluronic acid and moisturizing.

The Gamma Oligopeptide has a low molecular weight and high skin permeability, inhibits the activity of hyaluronic acid degrading enzyme and increases the concentration of hyaluronic acid below the skin layer. In addition, the gamma-oligopeptide functions to increase the intracellular collagen synthesis or to inhibit the activity of collagenase, an enzyme that degrades collagen, thereby maintaining the elasticity of the skin and ultimately improving wrinkles.

The content of the polygamma glutamic acid and the gamma oligopeptide contained in the liposome complex is preferably 0.01 to 8 wt% based on the total content of the liposome complex. If the content is less than 0.01% by weight, it may be difficult to achieve the aimed wrinkle-improving function. If the content exceeds 8% by weight, separation of liposome complex may occur.

Based on the total content of the liposome complex, 4 to 6% by weight of polygamma glutamic acid and 2 to 4% by weight of gamma oligopeptide. When the content is within the above range, the desired effects can be achieved without separating and precipitating the polygamma glutamic acid and the gamma oligopeptide.

The liposome complex is preferably contained in an amount of 0.1 to 10% by weight based on the total amount of the cosmetic composition. If the content is less than 0.1% by weight, it may be difficult to achieve a desired moisturizing and wrinkle-improving function. If the content is more than 10% by weight, it is difficult to uniformly disperse the cosmetic formulation and the liposome complex, have.

The cosmetic composition may additionally comprise a "dermatologically acceptable carrier" as known in the art. But are not limited to, purified water, oils, waxes, fatty acids, fatty acid alcohols, fatty acid esters, surfactants, moisture absorbers, thickeners, antioxidants, viscosity stabilizers, chelating agents, buffers, preservatives, lower alcohols, Types and concentrations vary. If necessary, the cosmetic composition may contain a whitening agent, a moisturizing agent, an anti-inflammatory agent, an antibacterial agent, an ultraviolet screening agent, an antibiotic, a perfume, and a dye, and these may be included in cosmetic compositions according to the present invention in amounts commonly used in cosmetics.

Specific examples of the oil include hydrogenated vegetable oil, pharmacopoeia, cottonseed oil, olive oil, palm oil, jojoba oil, and avocado oil. Examples of the wax include wax, wax, carnauba, candelilla, montan, ceresin, liquid paraffin , Lanolin may be used. As the fatty acid, stearic acid, linoleic acid, linolenic acid and oleic acid may be used. As the fatty acid alcohol, cetyl alcohol, octyldodecanol, oleyl alcohol, panthenol, lanolin alcohol, stearyl alcohol and hexadecanol may be used , Isopropyl myristate, isopropyl palmitate, butyl stearate and the like can be used as the fatty acid ester, but the present invention is not limited thereto.

Examples of the surfactant include anionic surfactants such as sodium stearate, sodium cetyl sulfate, polyoxyethylene lauryl ether phosphate, sodium N-acyl glutamate; Cationic surfactants such as stearyldimethylbenzylammonium chloride and stearyltrimethylammonium chloride; Amphoteric surfactants such as alkylaminoethylglycine hydrochloride and lecithin; Glycerin monostearate, sorbitan monostearate, propylene glycol monostearate, polyoxyethylene oleyl ether, polyethylene glycol monostearate, polyoxyethylene sorbitan monopalmitate, polyoxyethylene coconut fatty acid monoethaolarnide ), Polyoxypropylene glycol, polyoxyethylene castor oil, nonionic surfactants such as polyoxyethylene lanolin, and the like.

As the moisture absorbent, glycerin, 1,3-butylene glycol, propylene glycol may be used, and as the lower alcohol, ethanol and isopropanol may be used. Examples of thickeners include sodium alginate, sodium caseinate, gelatin agar, xanthan gum, starch, cellulose ethers (e.g., hydroxyethylcellulose, methylcellulose, carboxymethylcellulose, hydroxypropylmethylcellulose), polyvinylpyrrolidone, But are not limited to, polyvinyl alcohol, polyethylene glycol, and sodium carboxymethylcellulose. As the antioxidant, butylated hydroxytoluene, butylated hydroxyanisole, propyl gallate, citric acid, and ethoxyquin are usable. As the chelating agent, disodium edetate, ethanhydroxy diphosphate As the buffering agent, citric acid, sodium citrate, boric acid, borax, disodium hydrogen phosphate can be used. As the preservative, methyl parahydroxybenzoate, ethyl parahydroxybenzoate, dihydro Acetic acid, salicylic acid, benzoic acid, but are not limited thereto

The cosmetic composition may be applied to various formulations known in the art. For example, it may be formulated into a form of oil-in-water (O / W) type, w / o type, silicon w / Si type, ) Type, silicone water (W / Si) type and a water-free type, and the formulations are not particularly limited thereto.

Hereinafter, the present invention will be described in more detail with reference to test examples. However, the following test examples are provided for illustrating the present invention, and the present invention is not limited by the following test examples, and can be variously modified and changed.

Test Example One. Of poly-gamma glutamic acid (y-PGA) Solubility check and Precipitation evaluation

In order to evaluate the solubility and precipitation of poly (gamma glutamic acid), Examples 1 to 5 and Comparative Example 1 were prepared with the compositions and contents (unit: wt%) shown in Table 1 below.

Specifically, under the condition of 80 캜, the polar gum glutamic acid was dispersed in purified water until it became colorless transparent, cooled to room temperature again, and filled in a transparent glass container.

division Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Polygamma glutamic acid 4 8 10 12 14 0 Purified water 96 92 90 88 86 100

The purified water of Examples 1 to 5 and Comparative Example 1 was subjected to centrifugation at 3,000 rpm for 30 minutes using a centrifuge to determine the precipitation and dissolution of poly (gamma glutamic acid) and the presence or absence of precipitation of poly (gamma glutamic acid) 0 ° C, 25 ° C, 45 ° C, 55 ° C) for 3 months. The results are shown in Table 2 and FIG.

In Table 2, when the solution state is opaque depending on the degree of dissolution and precipitation, "⊚" indicates that the precipitation phenomenon occurs, and "?" Indicates that the solution is completely dissolved and not precipitated.

division Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Degree of dissolution . . . . . .

As shown in Table 2 and Fig. 1, it was confirmed that both of Examples 1 to 5 and Comparative Example 1 did not completely dissolve and precipitate poly-gamma glutamic acid transparently.

Test Example 2. Gamma-oligopeptide Solubility check and Precipitation evaluation

In order to evaluate the solubility and precipitation of gamma-oligopeptide, Examples 6 to 10 were prepared with the compositions and contents (unit: wt%) shown in Table 3 below, and Comparative Example 1, which is purified water, was used as a comparative example.

Specifically, under the condition of 80 캜, the gamma-oligopeptide was dispersed in the purified water until it became colorless transparent, cooled again to room temperature, and filled in a transparent glass container.

division Example 6 Example 7 Example 8 Example 9 Example 10 Comparative Example 1 Polygamma glutamic acid 4 8 10 12 14 0 Purified water 96 92 90 88 86 100

The purified water of Examples 6 to 10 and Comparative Example 1 was centrifuged at 3,000 rpm for 30 minutes using a centrifuge, and after the precipitation and dissolution of the gamma-oligopeptide were confirmed after the centrifugation, the presence or absence of precipitation of the gamma- 0 ° C, 25 ° C, 45 ° C, 55 ° C) for 3 months. The results are shown in Table 4 and FIG. 2.

In Table 4, when the solution state is opaque depending on the degree of dissolution and precipitation, "⊚" indicates that the precipitation phenomenon has occurred, and "?" Indicates that the solution is completely dissolved and not precipitated.

division Example 6 Example 7 Example 8 Example 9 Example 10 Comparative Example 1 Degree of dissolution . . . . . .

As shown in Table 4 and FIG. 2, it was confirmed that the gamma-oligopeptides in Examples 1 to 5 and Comparative Example 1 were completely dissolved completely and were not precipitated.

Test Example 3. Polygamma glutamic acid And Gamma oligopeptide Depending on the content Liposome Complex Cosmetics The preparation of the composition and Precipitation evaluation

Analysis of Test Examples 1 and 2 confirmed that the polygamma glutamic acid and gamma oligopeptide could be dissolved up to 14% by weight, respectively. In order to determine the optimum content for the preparation and stabilization of the liposome complex, Examples 11, 12, 13 and Comparative Example 2. For reference, the content of the base for preparing the liposome complex is limited, and the range in which the polygamma glutamic acid and the gamma oligopeptide can be applied is limited to 0 to 8 wt%.

(Unit: wt%) division Example 11 Example 12 Example 13 Comparative Example 2 Polygamma glutamic acid 4 6 2 0 Gamma oligopeptide 4 2 6 0 Liposome base 88.5 88.5 88.5 88.5 Other ingredients 3.5 3.5 3.5 11.5 Sum 100 100 100 100

In the same manner as in Test Examples 1 and 2, the presence or absence of polygamma glutamic acid and gamma oligopeptide was evaluated. The results are shown in Table 6 and FIG. In Table 6, when separation occurred, it was marked as "", and when separation did not occur, it was marked as". "

division Example 11 Example 12 Example 13 Comparative Example 2 Degree of separation . . . .

As shown in Table 6 and FIG. 3, it was confirmed that the polygamma glutamic acid and the gamma oligopeptide were not separated in Examples 11, 12 and Comparative Example 2, and the separation phenomenon occurred in the liposomized base in Example 13 . It is considered that this is a result of different effects on liposomization depending on the solubility, and when polygamma glutamic acid and gamma oligopeptide are blended to 4 wt% and 4 wt% respectively or 6 wt% and 2 wt% , It was confirmed that a stable liposome complex was formed.

Test Example 4. Polygamma glutamic acid And Gamma-oligopeptide Containing Liposome Complex Cosmetics Evaluation of skin stability of composition

In order to evaluate the skin safety of Examples 11 and 12 and Comparative Example 2 of Test Example 3 in which the separation of polygamma glutamic acid and gamma oligopeptide was not observed, a human skin patch test was conducted. After spraying the formulations of Examples 11 and 12 and Comparative Example 2 of Test Example 3 prescribed in the inside of the upper arm of 30 healthy adults who had no history of allergic contact dermatitis or atopic dermatitis and were found to have no skin disease at present, After the lapse of time, the patches were removed, and skin conditions immediately after the removal of the patches, 1 hour, and 24 hours were visually observed and evaluated according to the intensity of the stimulus. The results are shown in Table 7 below.

In Table 7, "+++" indicates severe stimulation, "++" indicates moderate stimulation, "+" indicates slight stimulation, and "-" indicates no stimulation depending on degree of stimulation.

division Example 11 Example 12 Comparative Example 2 Degree of stimulation - - -

As shown in Table 9, all of Examples 11, 12 and Comparative Example 2 proved to be a safe cosmetic composition without skin irritation due to no stimulation.

Test Example 5. Polygamma glutamic acid And Gamma-oligopeptide Containing Liposome Complex Cosmetics Evaluation of Skin Moisture of Composition

In order to compare the moisturizing effects of Examples 11 and 12 and Comparative Example 2 of Test Example 3 in which the polygamma glutamic acid and gamma oligopeptide liposome complexes were not separated, the skin conductivity was measured using a skin moisture meter (Corneometer) . In order to measure skin moisture, the inside of the arms of five test subjects were used as the measurement sites under the conditions of constant temperature and humidity (room temperature 20 to 25 ° C, relative humidity 40 to 60%), and Examples 11 and 12 and Comparative Example 2 of Test Example 3 were applied And measured by time. One measurement site was measured five times, and the average value was calculated by calculating the relative humidity increase rate (%) after use after use, and the results are shown in Table 8 below.

Time (minutes) Increase in skin moisturizing power (%) Example 11 Example 12 Comparative Example 2 Before use 0 0 0 30 minutes 33 39 28 1 hours 24 34 16 3 hours 19 26 10 5 hours 13 18 8

As shown in Table 8, in order to confirm the moisturizing effect when the liposome complex containing different amounts of polygamma glutamic acid and gamma oligopeptide was applied to the skin, the increase rate of skin moisturizing power was measured. As a result, By confirming that the increase rate of skin moisturizing power was increased in Examples 11 and 12, it was observed that both of Examples 11 and 12 were effective in increasing skin moisturizing power. Also, it was confirmed that the skin moisturizing ability was higher in Example 12.

Test Example 6. Polygamma glutamic acid And Gamma-oligopeptide Containing Liposome Complex Cosmetics Of the composition Measurement of collagen biosynthesis

In order to investigate the wrinkle-reducing effects of Examples 11 and 12 and Comparative Example 2 of Test Example 3 in which polygamma-glutamic acid and gamma-oligopeptide liposome complexes were not separated, a collagen biosynthesis accelerated test (Sircol ^TM Soluble Collagen Assay Kit ).

Cultured human fibroblasts were divided into 12-well culture dishes at a density of ¹ x 10 ⁵ cells / well in a medium containing 5% FBS, cultured for 24 hours, diluted by treatment concentration in a medium containing 2% FBS, And cultured for 48 hours. To confirm that the synthesis of collagen was actually increased in the fibroblast, a Sircol ^TM Soluble Collagen Assay Kit (Biocolor) was purchased and the amount of collagen was measured according to the experimental manual of the product manual. The Sircol ^™ dissolved collagen assay kit is a quantitative method that can be confirmed by the combination of collagen and staining reagent secreted into the culture medium during cell culture. Specifically, 1 ml of a dye reagent (a solution containing Sirius red reagent in Cicric acid) was added to 100 μl of the culture supernatant, and the mixture was allowed to react for 30 minutes. Sirius red was an anionic dye specifically bound to collagen. After reaction for 30 minutes, the reaction solution was centrifuged at 12,000 × g or more for 10 minutes to precipitate a collagen-dye complex. 1.0 ml of the alkali reagent was added to the precipitate and dissolved at room temperature for 5 minutes. The absorbance of the solution was measured at 540 nm using an absorption spectrophotometer (UVmax, Molecular Device, USA) and is shown in Table 9 below. The absorbance of the non-test group was compared to evaluate the promoting effect of collagen biosynthesis.

Measurement of collagen biosynthesis Average OD
(% of control) Test liquid concentration (W / W%) Control group 0.01 0.05 Example 11 0.471
(100) 0.518
(109.97) 0.563
(119.53) Example 12 0.471
(100) 0.511
(108.49) 0.552
(117.19)
Comparative Example 2
0.471
(100) 0.477
(101.27) 0.489
(103.63)

As shown in Table 9, it was observed that Examples 11 and 12 had excellent collagen biosynthesis effects in Examples 11 and 12 as compared with Comparative Example 2. Also, Example 11 showed better collagen biosynthesis than Example 12, but the difference from Comparative Example 2 was similar. Therefore, it can be seen that Example 12 of Test Example 3 has a blending ratio of poly (gamma glutamic acid) and gamma-oligo peptide which are more effective for improving the moisturizing power and wrinkle.

Test Example 7. Polygamma glutamic acid And Gamma-oligopeptide Containing Liposome Stability evaluation of cosmetics including complexes

A liposome complex (Example 12) was prepared as in Test Example 3 for application to water-based cosmetic formulations of polygamma glutamic acid and gamma-oligo peptide. Thereafter, application tests of polygamma glutamic acid and gamma-oligopeptide liposome complexes were conducted in W / O, W / Si, a non-aqueous cosmetic formulation, that is, a non-aqueous cosmetic formulation, which is a major concern of the present invention.

In Examples 14 to 16, 10% by weight of the liposome complex of Example 12 was applied to each cosmetic formulation, and the liposome complex was not applied to Comparative Examples 3 to 5. Examples 14 to 16 and Comparative Examples 3 to 5 were subjected to centrifugation at 3,000 rpm for 30 minutes using a centrifugal separator, and the stability was evaluated by evaluating the stability. The results are shown in Table 10 and FIG.

In Table 10, if separation occurred, it was marked as "", and if separation did not occur, it was marked as".

Fig. 4 is a graph showing the results of comparison between the results of Example 14 (W / O formulation + liposome), Example 15 (W / Si formulation + liposome), Example 16 (anhydrous formulation + liposome) (W / Si formulation) and Comparative Example 5 (anhydrous formulation) after centrifugation.

division Example 14
(W / O) Example 15
(W / Si) Example 16
(myriad) Comparative Example 3
(W / O) Comparative Example 4
(W / Si) Comparative Example 5
(myriad) Degree of separation . . . . . .

As shown in Table 10 and FIG. 4, it was confirmed that the polygamma glutamic acid and the gamma oligopeptide were not separated in the formulations of the cosmetic products of Examples 14 to 16.

Claims

A cosmetic composition comprising a liposome complex comprising polygamma glutamic acid and gamma oligopeptide.

The method according to claim 1,
Wherein the content of the polygamma glutamic acid and the gamma oligopeptide contained in the liposome complex is 0.01 to 8% by weight based on the total content of the liposome complex.

The method according to claim 1,
Based on the total content of the liposome complex,
4 to 6% by weight of polygamma glutamic acid, and
And 2 to 4% by weight of a gamma-oligopeptide.

The method according to claim 1,
Wherein the liposome complex is contained in an amount of 0.1 to 10% by weight based on the total amount of the cosmetic composition.

The method according to claim 1,
Wherein the cosmetic composition is for moisturizing or improving wrinkles.

The method according to claim 1,
Wherein the cosmetic composition is formulated into an oil-in-water (O / W) type, a water-in-oil type (W / O) type, a silicone water-in-water type (W / Si) type and a water-free type.