KR20170105806A - The sauce using fermented ginger soybean and method for preparation thereof - Google Patents
The sauce using fermented ginger soybean and method for preparation thereof Download PDFInfo
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- KR20170105806A KR20170105806A KR1020160028919A KR20160028919A KR20170105806A KR 20170105806 A KR20170105806 A KR 20170105806A KR 1020160028919 A KR1020160028919 A KR 1020160028919A KR 20160028919 A KR20160028919 A KR 20160028919A KR 20170105806 A KR20170105806 A KR 20170105806A
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- South Korea
- Prior art keywords
- ginger
- powder
- miso
- weight
- sauce
- Prior art date
Links
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Classifications
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- A23L11/20—
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/10—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L23/00—Soups; Sauces; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/50—Soya sauce
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/3262—Foods, ingredients or supplements having a functional effect on health having an effect on blood cholesterol
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/334—Foods, ingredients or supplements having a functional effect on health treating the effects of consuming alcohol, narcotics or other addictive behavior, e.g. treating hangover or reducing blood alcohol levels
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Seasonings (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a method for producing soy sauce, soybean paste, hot pepper paste, and a method for producing the same, which comprises aging a mixture of ginger meju, medicinal herb, salt, ginger powder and water using ginger Meju, And the effect of eliminating and purifying blood is improved.
Description
The present invention relates to soy sauce, soybean paste, kochujang, and a process for producing the soy sauce, soybean paste, and kochujang, which are improved in immunity and capable of improving hangover and enhancing the effect of green tea.
In general, Meju is one of the most important foods that should be indispensable to Korean dietary culture, because it is a source of various kinds of soy sauce, soybean paste, kochujang and so on after boiling soybeans, barley, wheat, and rice.
The beans used in the manufacture of meju contain not only very good nutrition but also good quality because they contain moisture 8.6%, protein 40%, fat 18%, fiber 3.5%, ash 4.6%, pentanoic acid 4.4% and sugar 7% Because it contains a large amount of vegetable protein, atherosclerosis, can be eaten by people who are concerned about heart disease, and smooth blood flow is also very functional food.
For example, genistein among isoflavones in soybean reduces cancer cell proliferation and exhibits anticancer activity that promotes normal cell division. Iriflavone, a derivative of isoflavone, Inhibit reabsorption and increase bone density to prevent osteoporosis.
These active ingredients in beans are also present in the process of fermentation with soybean paste and soy sauce. In addition, proteins are decomposed by the action of Aspergillus oryzae to produce various amino acids, and some amino acids and sugars A new antioxidant substance such as a substance on the melanoidin is produced. Polyphenols in soybean and melanoidin substances produced during fermentation process prevent oxidation of lipids such as linoleic acid in soy sauce and soybean paste to prevent rancidity and increase the content of unsaturated essential fatty acids.
As such, various active ingredients of traditional fermented foods such as soy sauce, soybean paste, and kochujang have been identified as meju, and they have been newly established as health foods. However, since they are different from the taste of modern westernized Korean people, Consumption is decreasing.
Among the fermented foods using meju, doenjang is a fermented soybean fermented food in Northeast Asia such as Korea, China, and Japan. It is a source of essential amino acids derived from proteins contained in soybean, Antioxidant efficacy, antimutagenic effect, anticancer efficacy, blood pressure lowering effect and anti-thrombogenic effect of doenjang extract have been reported as well as excellent nutrient It is an excellent traditional food that combines functional factors.
It is an object of the present invention to provide a method for producing soy sauce, soybean paste, and kochujang using ginger meju which is improved in immunity, can relieve hangover and improve the effect of green tea.
Another object of the present invention is to provide a method for producing ginger miso sauce using ginger miso, which is improved in immunity, can relieve hangover, and enhance the effect of green blood.
It is another object of the present invention to provide a method for manufacturing ginger kochujang using ginger miso so as to increase immunity, improve hangover resolution, and enhance the effect of green blood.
It is still another object of the present invention to provide ginger soy sauce using ginger Meju so that the immune system can be improved and the hangover can be relieved and the encephalopathy effect can be improved.
It is still another object of the present invention to provide ginger miso using ginger Meju so that the immune system is improved and the hangover is eliminated and the effect of blue blood is improved.
Still another object of the present invention is to provide a ginger miso sauce using ginger miso so that the immune system can be improved, hangover can be eliminated, and the effect of blue blood can be improved.
Still another object of the present invention is to provide ginger hot pepper paste using ginger miso so that the immunity is increased, the hangover is eliminated, and the effect of green tea is improved.
According to an aspect of the present invention, there is provided a method for manufacturing a sauce using ginger meju, comprising the steps of: (A) mixing ginger powder, high-malt barley and boiled soybeans and fermenting the ginger meju; (B) aging the mixture of the ginger bean, medicinal herb, salt, ginger powder and water; And (C) separating the aged aged material into liquid and solid.
In step (A), the ginger bean may be prepared by fermenting ginger powder at 10 to 25% by weight, gingerbread at 3 to 10% by weight and boiled beans at 70 to 85% by weight at 25 to 40 ° C.
In the step (B), the mixture may contain 30 to 40% by weight of ginger meju, 5 to 15% by weight of herb medicine, 2 to 10% by weight of salt, 3 to 5% by weight of ginger powder and 40 to 50% ; The medicinal herbs are mixed with Angelica gigas and licorice at a weight ratio of 1: 1 to 5.
In the step (C), the liquid may be ginger soy sauce, and the solid may be ginger miso.
According to another aspect of the present invention, there is provided a method of manufacturing a ginger miso sauce, wherein the ginger miso is mixed with a fermentation broth at a weight ratio of 1: 0.1 to 0.9.
The fermentation broth may be a mixture of 10 to 30% by weight of ginger powder, 10 to 30% by weight of fruits, 10 to 20% by weight of saccharides and 20 to 50% by weight of water; Wherein the fruit is at least one fruit selected from the group consisting of apple, pear, mandarin orange and persimmon, and the skin is removed; The saccharide may be at least one selected from the group consisting of sugar, fructose, honey, starch syrup, oligosaccharide and starch syrup.
According to another aspect of the present invention, there is provided a method of manufacturing ginger kochujang, wherein the ginger miso, fermentation broth, red pepper powder and ginger powder can be mixed.
The ginger powder used in the present invention may be dried after being steamed for 0.5 to 2 hours.
According to another aspect of the present invention, there is provided a ginger soy sauce comprising ginger meju, medicinal herb tea, salt, ginger powder, and water obtained by aging and then removing solid matter.
According to another aspect of the present invention, there is provided a ginger miso, which is obtained by aging ginger meju, herb medicine, salt, ginger powder and water, and then removing the liquid.
According to another aspect of the present invention, there is provided a ginger miso sauce wherein the ginger miso is mixed with a fermentation broth at a weight ratio of 1: 0.1 to 0.9.
According to another aspect of the present invention, there is provided a ginger hot pepper paste comprising ginger miso, fermented beverage, red pepper powder, and ginger powder.
The ginger miso, ginger soy sauce, ginger miso sauce, and ginger hot pepper paste using the ginger meju of the present invention show an excellent effect in prevention of a cold and prevention of diseases by enhancing the immune system, and it is effective for hangover, fatigue and liver disease when consuming alcohol The kidneys are not harmful to the body even when ingested by patients with bad kidneys.
In addition, when cooked together with vegetables such as spinach and parsley, the blood purifying effect that cleanse the blood is further improved, and it is excellent in sensibility, so that it can be enjoyed regardless of whether it is male,
The present invention relates to soy sauce, soybean paste, kochujang, and a process for producing the soy sauce, soybean paste, and kochujang, which are improved in immunity and capable of improving hangover and enhancing the effect of green tea.
Hereinafter, the present invention will be described in detail.
The method for producing soy sauce and soybean paste using ginger meju according to the present invention comprises the steps of: (A) preparing ginger meju by mixing ginger powder, high-malt barley, and boiled soybeans; (B) aging the mixture of the ginger bean, medicinal herb, salt, ginger powder and water; And (C) separating the aged aged material into a liquid and a solid.
First, in step (A), ginger powder, high-malt barley and boiled soybeans are mixed and fermented using rice straw at 25 to 40 ° C to prepare ginger meju.
As the ginger powder, the bitterness and odor peculiar to ginger are removed, and the powder is dried by heating at 100 to 150 ° C. for 0.5 to 2 hours in order to facilitate fermentation with boiled soybeans. When the ginger powder is used directly without adding the ginger powder, the bitterness and odor peculiar to the ginger can not be removed and the hangover resolution and the effect of the blue blood can not be improved.
The ginger powder is used in an amount of 10 to 25% by weight, preferably 15 to 20% by weight. If the content of the ginger powder is less than the lower limit value, a desired effect can not be obtained. If the content is higher than the upper limit value, the ginger powder content may be lowered.
In addition, the above-mentioned gomul broth is used as a powder to help produce ginger meju of excellent quality when boiled beans are mixed with soybean.
The high-malt barley is 3 to 10% by weight, preferably 5 to 8% by weight. If the content of goryeoborib is out of the above range, the taste may be deteriorated.
The above-mentioned boiled soybean is used as 70 to 85% by weight as a main ingredient of meju. Meju may not be produced when the content of soybeans is below the lower limit, and taste and functionality may be lowered when the content exceeds the upper limit.
The ginger meju produced in the step (A) is fermented for 2 to 4 months and used in the next step.
Next, in step (B), a mixture of the ginger bean, Chinese herbal medicine, salt, ginger powder and water is aged.
The ginger bean is used in an amount of 30 to 40% by weight, preferably 35 to 40% by weight, as prepared in the step (A). When the content of ginger bean is less than the above lower limit, a source having the desired superior functionality can not be obtained. If the content exceeds the upper limit, the taste may be lowered.
The medicinal herb manna is mixed with ginger meju and ginger powder to further improve functional and functional properties. In addition, it is possible to remove harmful components of ginger, which is a problem of ginger, .
The medicinal herb is mixed with Angelica gigas L. and licorice root at a weight ratio of 1: 1 to 5, preferably 1: 1 to 3. If the content of licorice is lower than the lower limit based on Angelica gigas, the functionality may not be improved. If the content exceeds the upper limit, toxicity may be caused.
The medicinal herbal composition is used in an amount of 5 to 15% by weight, preferably 8 to 10% by weight. If the content of the medicinal herb is less than the lower limit, the functional and functional properties may not be improved. If the content exceeds the upper limit, the functional and functional properties may be lowered.
The ginger powder is the same as the ginger powder used in step (A), and is used together with ginger meju and herbal medicine moon to further improve the immunity, which is a characteristic of the ginger itself.
When the content of ginger powder is less than the lower limit, the effect generated when the ginger powder is added may not be generated. When the content of ginger powder is higher than the upper limit, .
It is preferable that the salt is used in an amount of 2 to 10 wt% and the water is used in an amount of 40 to 50 wt% in order to separate into a liquid and a solid in the course of aging.
Next, in the step (C), when the mixture is aged (left for 4 to 8 months) in the step (B), the solid content is divided into a supernatant which is a liquid and a solid which is solid. Respectively.
Specifically, the liquid as the supernatant is used as ginger soy sauce, and the solid as the solid is made into ginger miso.
The present invention also provides a ginger miso sauce by mixing ginger miso and fermentation broth.
In the method of manufacturing the ginger miso sauce of the present invention, the ginger miso separated in the step (C) is mixed with the fermentation broth and fermented at 30 to 35 ° C for 1 to 30 days.
The fermentation liquid completely removes the bitter taste, odor and harmful components of the ginger, and is used with the ginger miso to further enhance the functionality and functionality.
The ginger sole is mixed with the fermentation broth at a weight ratio of 1: 0.1 to 0.9, preferably 1: 0.3 to 0.7. If the content of the fermentation broth is less than the lower limit based on the ginger miso, the improved effect can not be obtained to the target. If the content exceeds the upper limit, the functionality may be lowered.
The fermentation broth comprises 10 to 30% by weight, preferably 15 to 20% by weight, of ginger powder; 10 to 30% by weight, preferably 15 to 20% by weight of fruits; 10 to 20% by weight, preferably 10 to 15% by weight, of saccharides; And 20 to 50% by weight of water are mixed and fermentation is carried out at 30 to 35 ° C for 2 to 6 months until the sweetness disappears.
The ginger powder used in the fermentation liquid is the same as the ginger powder used in the step (A), and is used to improve the functionality of the ginger in the fermentation broth. When the content of the ginger powder is less than the lower limit value, the functionality of the ginger may not be improved. If the content of the ginger powder is higher than the upper limit, the functionality may be lowered.
When the content of the fruits is less than the lower limit, the sensory properties may not be improved. When the content of the fruit is higher than the upper limit, sweetness tends to be strong. Therefore, The taste of the sauce may not be revealed.
The fruits include at least one fruit selected from the group consisting of apple, pear, mandarin orange, and persimmon, and the fruit is preferably removed from the surface of the fruit.
The saccharide is a component necessary for fermentation. When the content of the saccharide is less than the lower limit value, fermentation may not be performed. If the sugar content is above the upper limit value, the sweet taste is strong and the taste of sauce such as meju, miso, soy sauce, etc. may not be revealed .
Although the fermentation is not performed for a certain period of time during the production of the ginger miso sauce, if the fermentation is performed, the ginger miso can be obtained which has a better effect.
In addition, the present invention provides ginger hot pepper paste by mixing ginger miso, red pepper powder, fermentation liquid and the like.
The method of manufacturing ginger kochujang of the present invention comprises: 5 to 20% by weight of ginger miso separated in step (C), 10 to 30% by weight of fermentation broth, 30 to 45% by weight of red pepper powder, 5 to 20% To 10% by weight based on the total weight of the composition.
Ginger soybean paste is used to enhance the functionality and functionality of kochujang. When the content is out of the above range, both functionality and functionality may be lowered.
In addition, the fermentation broth can be used with the fermented broth as the fermentation broth used in the ginger miso sauce, thereby enhancing the functionality and removing the fishy flesh during ingestion with the ginger miso.
If the content of the fermentation broth is less than the lower limit value, the functionality and the functionality may be deteriorated. If the content is above the upper limit value, the functionality may be deteriorated.
Also, the ginger powder is the same as the ginger powder used in the step (A), and can be used with ginger miso to remove the fishy flesh of the fish during ingestion and kill the microorganisms contained in the fish.
If the content of ginger powder is less than the above lower limit, fish can not be killed and microorganisms can be killed. If the ginger powder is above the upper limit, the taste may be deteriorated.
The red pepper powder is a main ingredient for producing red pepper paste. If the content is below the lower limit, the red pepper paste may not be produced. If the content is above the upper limit, the red pepper powder may taste.
The salt is also a main ingredient for producing kochujang. When the content is less than the lower limit, the storage period can be shortened. If the salt is above the upper limit, salty taste can be strong.
As described above, according to the present invention, when the steps (A) to (C) are carried out, ginger soy sauce and ginger miso can be obtained. When the ginger miso is mixed with the fermentation broth, ginger miso sauce can be obtained, It is possible to obtain ginger kochujang by mixing with fermentation broth and red pepper powder.
It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.
Production Example 1. Preparation of fermentation broth
20% by weight of ginger powder, 10% by weight of peeled apple, 10% by weight of peeled mandarin, 15% by weight of crude wax and 45% by weight of water were mixed and fermented at 30 ° C for 6 months to prepare a fermented liquid Respectively.
Production Example 2. Preparation of fermentation broth
The fermentation broth was prepared in the same manner as in Preparation Example 1 except that the ginger powder was omitted and 20% by weight of the peeled apple, 20% by weight of the peeled mandarin, 15% by weight of crude white rice and 45% by weight of water were mixed.
Example 1. Preparation of ginger liver
Manufacture of ginger meju
15% by weight of ginger powder, 8% by weight of high-malt barley, and 77% by weight of boiled beans were fermented at 30 占 폚 to prepare ginger meju.
Ginger soy sauce manufacturing
10% by weight of ginseng herb, 10% by weight of salt, 5% by weight of ginger powder and 40% by weight of water were mixed with 35% by weight of ginger mussel and 6% The supernatant was separated into a liquid phase supernatant and a solid solid phase. Only the supernatant except for the solid content was separated to obtain ginger liver.
Example 2. Preparation of ginger miso
Except that the solid content except for the supernatant was separated to obtain ginger miso.
Example 3: Preparation of ginger miso sauce
The fermentation broth prepared according to Example 2 was mixed with the fermented broth prepared according to Preparation Example 1 at a weight ratio of 1: 0.5 and fermented at 30 ° C for 1 day to prepare a ginger miso sauce.
Example 4. Preparation of ginger hot pepper paste
A ginger kochujang was prepared by mixing 20% by weight of the ginger mustard of Example 2, 20% by weight of the fermentation broth prepared according to Preparation Example 1, 40% by weight of red pepper powder, 10% by weight of ginger powder and 10%
Comparative Example 1. Korean Traditional Medicine
The ginger miso sauce was prepared by omitting the Chinese medicinal moon character when manufacturing the ginger miso.
Comparative Example 2. Using common meju
The bean sauce was prepared according to the above Example 3 except that the bean sauce was prepared using 10 wt% of goryongbori rice and 90 wt% of boiled beans, without using ginger powder.
Comparative Example 3. Omission of ginger powder
The bean sauce was prepared according to the above Example 3 except that the ginger powder was omitted when preparing the ginger miso.
Comparative Example 4.
A fermented liquid prepared according to Production Example 2 was used to prepare a ginger miso sauce according to the above Example 3.
< Test Example >
Test Example One. Anti-complement Activity evaluation
Antibody activity was evaluated to confirm immunostimulatory effect. The complement fixation test method based on the degree of erythrocyte hemolysis due to the residual complement after complement consumption by the sample (10-fold dilution) using the Mayer method was used to determine the activity of the complement system. , And PSK (polysaccharide-K) derived from a commercially available immunoglobulin-active polysaccharide, Mince mushroom, was used as a positive control. As a negative control, distilled water without addition of a sample was used. Activation level of each sample was confirmed by setting ITCH 50 0% in the negative control group, and it is shown in Table 1 below.
As shown in Table 1, it was confirmed that the soy sauce, miso, doenjang sauce, and kochujang produced according to Examples 1 to 4 of the present invention had better immunity enhancing effects than Comparative Examples 1 to 4.
Test Example 2. Assessment of cytokine production capacity
Macrophages regulate the immune response by secretion of various cytokines in the course of phagocytosis and elimination of germs and foreign substances, and play a pivotal role in the immune function to the antigen. They are related to the antigen presentation and nonspecific immune action of lymphocytes , And exhibit direct injury activity against tumor cells. In addition, TLR (toollike receptor) -responsive substances activate macrophages and produce cytokines such as IL-1, IL-6, IL-10, IL-12 and TNF-α for proliferation and phagocytosis of T cells and B cells It is known to produce cain.
In general, IL-1, IL-6 and TNF-α are representative cytokines induced by macrophages, which play a pivotal role in the inflammatory response to bacterial infection and are known to increase in inflammatory lesions.
Sources (10-fold dilutions) prepared according to Examples and Comparative Examples were evaluated for cytokine (TNF-a) production stimulation by sandwich ELISA using a macrophage obtained from abdominal cavity of C57B6 mice , Which is shown in Table 2 below. Negative control was measured without any addition, and cytokine (TNF-a) production stimulation was measured by adding lipopolysaccharide (LPS) as a positive control.
As shown in Table 2 above, soy sauce, miso, miso sauce, and kochujang prepared according to Examples 1 to 4 of the present invention show higher TNF-α production stimulating ability than Comparative Examples 1 to 4, .
Test Example 3. Alcohololytic enzyme Acetaldehyde ( ADH ) Activity measurement
The ADH activity measurement was performed according to the method provided using an acetaldehyde assay kit. Dilute the source (experimental group) and the control (purified water) in a digestion buffer and add 50 μl each to 96 multi-well plates. Mix the mixture with 82 μL of degradation buffer, 8 μL of developer, and 10 μL of isopropanol. Lt; / RTI > After incubation at 37 ° C for 3 minutes, the reaction was carried out and the amount of ADH produced was measured. The absorbance was measured at 450 nm using an ultraviolet spectrophotometer.
After 30 minutes of incubation, the amount of ADH produced was measured and the alcoholysis activity was determined using the two equations given in the kit.
When the absorbance of the solvent control was taken as 1, the relative absorbance of the composition was indicated, the control group was using purified water and the activity of the composition was expressed as a relative ratio to the control group. The results are shown in Table 3 below.
As shown in Table 3 above, the soy sauce, miso, doenjang sauce, and kochujang produced according to Examples 1 to 4 of the present invention were found to have higher ADH activity than Comparative Examples 1 to 4. It can be seen that the sources of Examples 1 and 2 have the toxic effect of acetaldehyde which causes hangover, fatigue and liver disease when consumed with alcohol. That is, since the effect of accelerating the activity of the alcoholase, ADH, is excellent, ethanol is rapidly decomposed into acetic acid, which is the final metabolite, so that prevention and improvement of various diseases caused by alcohol consumption can be expected.
Test Example 4. Sensory Test
Samples prepared in the Examples and Comparative Examples were stored for 1 day and then tested by 20 professional panelists. Then, the sensory evaluation was carried out using the 9-point scale method (as the degree was increased to 9 points) 4].
- Incense, taste, color and overall likelihood: 1 = very bad, 9 = very good
As shown in Table 4, it was confirmed that the soy sauce, miso, miso sauce, and red pepper sauce prepared according to Example 1 of the present invention were superior in flavor, taste, color and overall acceptability to those of Comparative Examples 1 to 4.
Claims (16)
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| KR20240092653A (en) | 2022-12-14 | 2024-06-24 | 농업회사법인주식회사해피피플 | Manufacturing method of red pepper paste containing peanut sprout powder |
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