KR20170105722A - An Aptamer-coated microneedle -Based Patch for detecting specific material - Google Patents

An Aptamer-coated microneedle -Based Patch for detecting specific material Download PDF

Info

Publication number
KR20170105722A
KR20170105722A KR1020160028648A KR20160028648A KR20170105722A KR 20170105722 A KR20170105722 A KR 20170105722A KR 1020160028648 A KR1020160028648 A KR 1020160028648A KR 20160028648 A KR20160028648 A KR 20160028648A KR 20170105722 A KR20170105722 A KR 20170105722A
Authority
KR
South Korea
Prior art keywords
aptamer
patch
skin
present
microneedle
Prior art date
Application number
KR1020160028648A
Other languages
Korean (ko)
Inventor
손인식
Original Assignee
주식회사 넥스모스
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 주식회사 넥스모스 filed Critical 주식회사 넥스모스
Priority to KR1020160028648A priority Critical patent/KR20170105722A/en
Publication of KR20170105722A publication Critical patent/KR20170105722A/en

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • A61K9/0021—Intradermal administration, e.g. through microneedle arrays, needleless injectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
    • A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
    • A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
    • A61M2037/0023—Drug applicators using microneedles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/20—Dermatological disorders

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medical Informatics (AREA)
  • Anesthesiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)

Abstract

The present invention relates to an aptamer-coated microneedle-based patch for detecting a specific material. The patch of the present invention is much smaller in size than that of an antibody material, and can couple a number of aptamers to a relatively large number of microneedle tip surfaces. In addition, since aptamers for various types of bio-markers can be attached at one time, various types of materials can be detected (multiplexing), and a microneedle tip-based skin patch can be used as a protein chip using the aptamer.

Description

앱타머 코팅된 마이크로니들 기반 특정 물질 검출용 패치{An Aptamer-coated microneedle -Based Patch for detecting specific material}An aptamer-coated microneedle-based Patch for detecting specific material based on an aptamer-

본 발명은 앱타머 코팅된 마이크로니들 기반 특정 물질 검출용 패치에 관한 것이다.The present invention relates to a patch for detecting an aptamer-coated micro-needle-based specific substance.

피부 질환은 오늘날 세계에서 주요 건강 관리 도전을 대표한다. 미국(국립암연구소, www.cancer.gov)에서 해마다 새로이 진단되는 백만 건 이상의 피부암과 더불어, 피부 질환을 예측 및 진단하는 것은 그것의 관리 측면에서 중요하다. 현재의 진단 방법은 시각적인 관찰 및 생검에 주로 의존한다. 그러나, 시각적인 관찰에 의존하는 검출 방법은 피부 상태 또는 질환을 진단하는데 반드시 효과적인 것은 아니며, 임상적인 징후가 나타날 때까지는 위험(risk) 또는 질환을 검출하지 못한다. 게다가, 생검과 같은 침습성 방법은 시험 대상에 대한 외상뿐만 아니라, 감염의 가능성도 증가시킨다. 또한 상기 방법은 안전하게 실시되기 위해서 의사에 의해 수행되어야 하며, 일반적으로 반응에 관여하는 세포인 피부 표면에 있는 풍부한 세포 샘플을 보통 제공하지 않는다.Skin diseases represent a major healthcare challenge in the world today. Along with more than one million new skin cancers diagnosed annually in the US (National Cancer Institute, www.cancer.gov), predicting and diagnosing skin disorders is important in its management. Current diagnostic methods rely mainly on visual observation and biopsy. However, detection methods that rely on visual observations are not necessarily effective in diagnosing a skin condition or disease, and do not detect a risk or disease until clinical manifestations occur. In addition, invasive methods such as biopsy increase the likelihood of infection as well as trauma to the test subject. In addition, the method should be performed by a physician in order to be safely performed, and usually does not provide a rich cell sample on the surface of the skin, which is generally a cell involved in the response.

따라서 피부 상태 및 질환을 진단 및 모니터링하는 비침습성 방법은 환자의 관리를 위한, 그리고 기존 및 새로운 치료제, 피부 관리 제품 및 피부 관리 섭생의 효능을 평가하기 위한 중요한 수단을 나타낸다. 게다가, 상기방법은 피부 질환의 발생에 대한 시험 대상의 유전적 소인뿐만 아니라, 시험 대상의 피부 상태를 기초로 하는 특이적인 유전적 변화에 관하여 중요한 정보를 제공할 수 있다. 상기 유전적 변화를 동정하는 것은 잠재적인 약물 타겟 및 예방적 조치를 동정하고 특정 치료제, 피부 관리 제품 또는 섭생에 대해 사람이 실제적으로 반응을 하는지를 결정하는데 중요할 수 있다. 뿐만 아니라, 검출 및 진단 방법은 그러한 치료, 제품 및 조치의 안전성을 평가하는데 중요하다.Non-invasive methods for diagnosing and monitoring skin conditions and diseases therefore represent an important means for the management of patients and for evaluating the efficacy of existing and new treatments, skin care products and skin care regimens. In addition, the method can provide important information about specific genetic changes based on the skin condition of the test subject, as well as the genetic susceptibility of the test subject to the occurrence of skin disease. Identifying the genetic alteration may be important in identifying potential drug targets and preventative measures and determining whether a person actually responds to a particular therapeutic agent, skin care product or regimen. In addition, detection and diagnostic methods are important in assessing the safety of such treatments, products and measures.

또한, 국소적인 피부질환은 물론 다양한 질병상태에서 피부의 물질의 조성이 바뀌는 것이 보고되고 있다. 지질, 구조단백질, 염증성물질, 핵산, 대사산물 등 여러물질이 질병상태에 따라 피부에서 다양하게 검출되는 것으로 알려져있다. 현재 아토피성 피부염, 흑색종, 피부의 세균성 염증 외에도 알츠하이머병, 파킨슨씨병, 유방암, 심혈관계 질환, 당뇨병, 약물 중독등의 다양한 질환에서 피부의 바이오마커 분석이 이루어지고 있다. 그러나, 대부분의 경우 대단히 침습적인 피부생검(Skin Biopsy)이 사용되고 있다. 비침습적 방법으로 Iontophoresis, Microdialysis, Tape stripping, Ultrasound, Microneedle등이 사용되고 있으나, 매우 효율은 낮은 편이다 (Paliwal et al., 2013 Diagnostic opportunities based on skin biomarkers. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 50:546-556). In addition, it has been reported that the composition of skin material changes in various disease states as well as in local skin diseases. It is known that various substances such as lipids, structural proteins, inflammatory substances, nucleic acids, metabolites and the like are variously detected in the skin depending on the disease state. In addition to atopic dermatitis, melanoma and bacterial inflammation of the skin, biomarker analysis of skin has been performed in various diseases such as Alzheimer's disease, Parkinson's disease, breast cancer, cardiovascular disease, diabetes, drug addiction and the like. However, in most cases, a very aggressive skin biopsy is being used. In the noninvasive method, Iontophoresis, Microdialysis, Tape stripping, Ultrasound, and Microneedle are used, but they are very low in efficiency (Paliwal et al., 2013 Diagnostic opportunities based on biomarkers. Federation for Pharmaceutical Sciences 50: 546-556).

[선행 특허 문헌][Prior Patent Literature]

대한민국 특허공개번호 제1020120006945호Korean Patent Publication No. 1020120006945

본 발명은 상기의 문제점을 해결하고 필요성에 의하여 안출된 것으로서 본 발명의 목적은 다양한 물질의 검출에 이용하기 위한 패치를 제공하는 것이다. DISCLOSURE OF THE INVENTION The present invention has been made in view of the above problems, and it is an object of the present invention to provide a patch for use in the detection of various substances.

상기의 목적을 달성하기 위하여 본 발명은 a) 앱타머에 아민기를 결합시키는 단계;b) 마이크로니들 팁 표면을 프라즈마 산화(plasma oxidation)로 실라놀(silanol)을 붙인 후, 그 하이드록실(hydroxyl)기에 3-glycidoxypropyltrimethoxysilane (3-GPTMS)으로 실란처리(silanizing)한 후, 상기 3-GPTMS에 포함된 에폭시(epoxy)기에 상기 앱타머에 결합된 아민기를 결합시키는 단계를 포함하는 앱타머가 코팅된 마이크로니들 기반 특정 물질 검출용 패치 제조방법을 제공한다.In order to achieve the above object, the present invention provides a method for preparing a metal oxide nanoparticle comprising the steps of: a) bonding an amine group to an aptamer, b) attaching a silanol on the surface of a micro needle tip by plasma oxidation, Silane treatment with 3-glycidoxypropyltrimethoxysilane (3-GPTMS) in the 3-GPTMS, followed by binding an amine group bonded to the aptamer to the epoxy group contained in the 3-GPTMS. Based specific substance detection method.

또 본 발명은 상기 본 발명의 방법에 의하여 제조된 앱타머가 코팅된 마이크로니들 기반 특정 물질 검출용 패치를 제공한다.In addition, the present invention provides a patch for detecting a specific material based on an aptamer coated micro-needle produced by the method of the present invention.

본 발명의 일 구현예에 있어서, 상기 피부 진단 패치는 마이크로니들 팁 표면에 앱타머가 부착되고 상기 앱타머의 말단에는 진단 가능한 성분이 부착될 수 있는 것을 특징으로 하나 이에 한정되지 아니한다. 상기 진단 가능한 성분은 생리 활성 물질, 단백질, 펩타이드, DNA 또는 RNA인 것이 바람직하나 이에 한정되지 아니한다.In an embodiment of the present invention, the skin diagnosis patch is characterized in that an aptamer is attached to the surface of the micro needle tip and a diagnostic component is attached to the end of the aptamer. The diagnosable component is preferably a physiologically active substance, a protein, a peptide, DNA or RNA, but is not limited thereto.

본 발명의 앱타머가 코팅된 마이크로니들 제작과 관련하여서는 Regarding the production of the aptamer coated micro needle according to the present invention

Brain-machine interfaces (BMIs)나 피부를 통한 효과적인 약물의 delivery를 위해 그간 여러 종류의 microneedle이 개발되었고 실제 두피를 통한 뇌파의 측정등에 테스트 되었다.Several microneedles have been developed to deliver effective drug delivery through brain-machine interfaces (BMIs) and skin, and have been tested for measurement of brain waves through the scalp.

한 예로 2011년에 발표된 논문(Ami, Y et al, J of Micro/Nanolithography, MEMS, and MOEMS. 10 (1), 011503, March 2011)을 참고하면 Polydimethylsiloxane (PDMS)를 재료로 두께 50 um x 길이 200 um로 끝이 뾰족한 스킨침투용 마이크로니들을 성공적으로 개발한 것을 볼 수 있다.(도 1).As an example, reference is made to a paper published in 2011 (Ami, Y et al, J of Micro / Nanolithography, MEMS, and MOEMS.10 (1), 011503, March 2011). Polydimethylsiloxane (PDMS) It can be seen that microneedles for skin infiltration with a sharp point of 200 μm in length have been successfully developed (FIG. 1).

본 발명의 앱타머가 코팅된 마이크로니들 제조의 한 예로는 Microneedle을 구성하는 PDMS의 표면을 Plasma oxidation을 통해 Silanol (SiOH)을 형성한다. 이 Hydroxyl 기를 epoxy기를 가지고 있는3-glycidoxypropyltrimethoxysilane (3-GPTMS) 로 silanizing후이 expoxy기에 앱타머에 결함 시켜둔 amine기 (도 2) 를 붙입으로서 Microneedle의 표면에 앱타머를 붙일 수 있다 (도 3).One example of the production of the aptamer coated micro needle according to the present invention is to form silanol (SiOH) through plasma oxidation of the surface of PDMS constituting microneedle. This hydroxyl group is silanized with 3-glycidoxypropyltrimethoxysilane (3-GPTMS) having an epoxy group, and an aptamer can be attached to the surface of Microneedle by attaching an amine group (FIG. 2) deficient in the aptamer to the expoxy group (FIG.

본 발명을 통하여 알 수 있는 바와 같이, 본 발명에서 앱타머라는 single strand DNA나 RNA의 삼차원구조를 이용해 특정 물질을 검출하는 방법으로 항원-항체 반응과 비슷하나 물질의 사이즈가 훨씬 작고 상대적으로 많은 수의 마이크로니들 팁 표면에 많은 수의 앱타머를 결합시킬 수 있다는 장점이 있다. As can be seen from the present invention, in the present invention, aptamera is a method of detecting a specific substance using a three-dimensional structure of single strand DNA or RNA, which is similar to an antigen-antibody reaction, but the size of the substance is much smaller and relatively large A large number of aptamers can be bonded to the surface of the micro needle tip.

또한 여러 종류의 바이오 마커에 대한 앱타머를 한꺼번에 붙일 수 있으므로 동시에 여러 종류의 물질을 검출할 수 있어서(Multiplexing), 마이크로니들 팁 기반 스킨패치도 앱타머를 이용한 단백질 칩으로 사용할 수 있다.In addition, since a variety of biomarkers can be attached at the same time, various types of materials can be detected at the same time (Multiplexing), and a micro needle tip-based skin patch can also be used as a protein chip using an aptamer.

도 1은 PDMS를 이용한 microneedle의 제작과 피부의 층에 맞추어 본 구조.
도 2는 Amine (NH2-)기를 결합시킨 앱타머 그림,
도 3은 Microneedle tip 표면을 plasma oxidation 으로 silanol을 붙인후, hydroxyl 기에 3-GPTMS로 silanizing후 3-GPTMS에 포함된epoxy기에 앱타머에 결합된 amine 기를 결합시키는 방법.Fig. 1 shows the fabrication of the microneedle using PDMS and the structure according to the skin layer.
FIG. 2 is a schematic view showing an aptamer binding with an Amine (NH 2 -) group,
FIG. 3 shows a method of silanizing a microneedle tip surface by plasma oxidation, silanizing 3-GPTMS in a hydroxyl group, and then bonding an amine group bonded to an aptamer to an epoxy group contained in 3-GPTMS.

이하 비한정적인 실시예를 통하여 본 발명을 더욱 상세하게 설명한다. 단 하기 실시예는 본 발명을 예시하기 위한 의도로 기재한 것으로서 본 발명의 범위는 하기 실시예에 의하여 제한되는 것으로 해석되지 아니한다.The present invention will now be described in more detail by way of non-limiting examples. The following examples are intended to illustrate the present invention and the scope of the present invention is not to be construed as being limited by the following examples.

본 발명의 앱타머가 코팅된 마이크로니들 제작과 관련하여서는 Regarding the production of the aptamer coated micro needle according to the present invention

Brain-machine interfaces (BMIs)나 피부를 통한 효과적인 약물의 delivery를 위해 그간 여러 종류의 microneedle이 개발되었고 실제 두피를 통한 뇌파의 측정등에 테스트 되었다.Several microneedles have been developed to deliver effective drug delivery through brain-machine interfaces (BMIs) and skin, and have been tested for measurement of brain waves through the scalp.

한 예로 2011년에 발표된 논문(Ami, Y et al, J of Micro/Nanolithography, MEMS, and MOEMS. 10 (1), 011503, March 2011)을 참고하면 Polydimethylsiloxane (PDMS)를 재료로 두께 50 um x 길이 200 um로 끝이 뾰족한 스킨침투용 마이크로니들을 성공적으로 개발한 것을 볼 수 있다.(도 1).As an example, reference is made to a paper published in 2011 (Ami, Y et al, J of Micro / Nanolithography, MEMS, and MOEMS.10 (1), 011503, March 2011). Polydimethylsiloxane (PDMS) It can be seen that microneedles for skin infiltration with a sharp point of 200 μm in length have been successfully developed (FIG. 1).

본 발명의 앱타머가 코팅된 마이크로니들 제조의 한 예로는 Microneedle을 구성하는 PDMS의 표면을 Plasma oxidation을 통해 Silanol (SiOH)을 형성한다. 이 Hydroxyl 기를 epoxy기를 가지고 있는3-glycidoxypropyltrimethoxysilane (3-GPTMS) 로 silanizing후이 expoxy기에 앱타머에 결함 시켜둔 amine기 (도 2) 를 붙입으로서 Microneedle의 표면에 앱타머를 붙일 수 있다 (도 3).One example of the production of the aptamer coated micro needle according to the present invention is to form silanol (SiOH) through plasma oxidation of the surface of PDMS constituting microneedle. This hydroxyl group is silanized with 3-glycidoxypropyltrimethoxysilane (3-GPTMS) having an epoxy group, and an aptamer can be attached to the surface of Microneedle by attaching an amine group (FIG. 2) deficient in the aptamer to the expoxy group (FIG.

Claims (4)

a) 앱타머에 아민기를 결합시키는 단계;b) 마이크로니들 팁 표면을 프라즈마 산화(plasma oxidation)로 실라놀(silanol)을 붙인 후, 그 하이드록실(hydroxyl)기에 3-glycidoxypropyltrimethoxysilane (3-GPTMS)으로 실란처리(silanizing)한 후, 상기 3-GPTMS에 포함된 에폭시(epoxy)기에 상기 앱타머에 결합된 아민기를 결합시키는 단계를 포함하는 앱타머가 코팅된 마이크로니들 기반 특정 물질 검출용 패치 제조방법.a) attaching an amine group to an aptamer; b) attaching silanol to the surface of the tip of the micro needle tip by plasma oxidation, and then adding 3-glycidoxypropyltrimethoxysilane (3-GPTMS) to the hydroxyl group Silanizing and then binding an amine group bonded to the aptamer to an epoxy group contained in the 3-GPTMS. 제1항의 방법에 의하여 제조된 앱타머가 코팅된 마이크로니들 기반 특정 물질 검출용 패치.A patch for detecting a specific material based on an aptamer coated micro-needle produced by the method of claim 1. 제2항에 있어서, 상기 패치는 마이크로니들 팁 표면에 앱타머가 부착되고 상기 앱타머의 말단에는 진단 가능한 성분이 부착될 수 있는 것을 특징으로 하는 마이크로니들 기반 특정 물질 검출용 패치. 3. The patch of claim 2, wherein the patch comprises an aptamer attached to the surface of the micro needle tip and a diagnostic component attached to the end of the aptamer. 제3항에 있어서, 상기 진단 가능한 성분은 생리 활성 물질, 단백질, 펩타이드, DNA 또는 RNA인 것을 특징으로 하는 마이크로니들 기반 특정 물질 검출용 패치. [4] The patch according to claim 3, wherein the diagnosable component is a physiologically active substance, a protein, a peptide, DNA, or RNA.
KR1020160028648A 2016-03-10 2016-03-10 An Aptamer-coated microneedle -Based Patch for detecting specific material KR20170105722A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020160028648A KR20170105722A (en) 2016-03-10 2016-03-10 An Aptamer-coated microneedle -Based Patch for detecting specific material

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020160028648A KR20170105722A (en) 2016-03-10 2016-03-10 An Aptamer-coated microneedle -Based Patch for detecting specific material

Publications (1)

Publication Number Publication Date
KR20170105722A true KR20170105722A (en) 2017-09-20

Family

ID=60033704

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020160028648A KR20170105722A (en) 2016-03-10 2016-03-10 An Aptamer-coated microneedle -Based Patch for detecting specific material

Country Status (1)

Country Link
KR (1) KR20170105722A (en)

Similar Documents

Publication Publication Date Title
US20190328938A1 (en) Method for fabricating microneedle-based diagnostic skin patch coated with aptamer and patch
Altuna et al. SU-8 based microprobes for simultaneous neural depth recording and drug delivery in the brain
Coffey et al. Rapid and selective sampling of IgG from skin in less than 1 min using a high surface area wearable immunoassay patch
JP7479726B2 (en) Minimally invasive atopic dermatitis testing method using a microneedle patch, and a minimally invasive atopic dermatitis testing kit including a microneedle patch
EP3757226A3 (en) A multi-protein biomarker assay for brain injury detection and outcome
TW201039798A (en) Switch probe for multiple electrode measurement of impedance
Linh et al. Bioinspired plasmonic nanoflower-decorated microneedle for label-free intradermal sensing
US20210321916A1 (en) Actuator system
Bandopadhyay et al. Recent developments in diagnosis of epilepsy: scope of MicroRNA and technological advancements
CN102525421A (en) Detecting micro-needle with strengthened Raman and fluorescence signal and preparation method thereof
Seeni et al. Iron oxide nanoparticle-powered micro-optical coherence tomography for in situ imaging the penetration and swelling of polymeric microneedles in the skin
Chang et al. Wearable devices for single-cell sensing and transfection
CN107912020B (en) Method for detecting pulsatile dynamics of optic nerve sheath and apparatus therefor
US20210386373A1 (en) Analyte detection system
Shaikh et al. Portable pen-like device with miniaturized tactile sensor for quantitative tissue palpation in oral cancer screening
Pedrosa et al. In-service characterization of a polymer wick-based quasi-dry electrode for rapid pasteless electroencephalography
Lu et al. Microneedle-based device for biological analysis
KR20170041375A (en) A Microneedle skin patch functionalized by Aptamer-coated carbon nanotube for early diagnosis of various diseases
WO2017007271A1 (en) Aptamer-coated microneedle-based diagnostic skin patch
KR20170105722A (en) An Aptamer-coated microneedle -Based Patch for detecting specific material
US20200054256A1 (en) Method for fabricating microneedle-based diagnostic skin patch coated with aptamer and patch
US20220346679A1 (en) Analyte measurement system
US20220361803A1 (en) System and methods for impedance-based non-invasive intracranial monitoring
Mamun et al. In-Plane Si Microneedles: Fabrication, Characterization, Modeling and Applications
Wang et al. Advances in microneedles for transdermal diagnostics and sensing applications