KR20170041375A - A Microneedle skin patch functionalized by Aptamer-coated carbon nanotube for early diagnosis of various diseases - Google Patents
A Microneedle skin patch functionalized by Aptamer-coated carbon nanotube for early diagnosis of various diseases Download PDFInfo
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- KR20170041375A KR20170041375A KR1020150140741A KR20150140741A KR20170041375A KR 20170041375 A KR20170041375 A KR 20170041375A KR 1020150140741 A KR1020150140741 A KR 1020150140741A KR 20150140741 A KR20150140741 A KR 20150140741A KR 20170041375 A KR20170041375 A KR 20170041375A
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- aptamer
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- carbon nanotube
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/44—Detecting, measuring or recording for evaluating the integumentary system, e.g. skin, hair or nails
- A61B5/441—Skin evaluation, e.g. for skin disorder diagnosis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0002—Remote monitoring of patients using telemetry, e.g. transmission of vital signals via a communication network
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/05—Detecting, measuring or recording for diagnosis by means of electric currents or magnetic fields; Measuring using microwaves or radio waves
- A61B5/053—Measuring electrical impedance or conductance of a portion of the body
- A61B5/0531—Measuring skin impedance
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
- A61B5/14507—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue specially adapted for measuring characteristics of body fluids other than blood
- A61B5/1451—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue specially adapted for measuring characteristics of body fluids other than blood for interstitial fluid
- A61B5/14514—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue specially adapted for measuring characteristics of body fluids other than blood for interstitial fluid using means for aiding extraction of interstitial fluid, e.g. microneedles or suction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/68—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
- A61B5/6801—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be attached to or worn on the body surface
- A61B5/683—Means for maintaining contact with the body
- A61B5/6832—Means for maintaining contact with the body using adhesives
- A61B5/6833—Adhesive patches
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/68—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
- A61B5/6846—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive
- A61B5/6847—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive mounted on an invasive device
- A61B5/685—Microneedles
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- Heart & Thoracic Surgery (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
Description
The present invention relates to a micro needle skin patch functionalized with an aptamer-coated carbon nanotube for early diagnosis of various diseases.
Skin diseases represent a major healthcare challenge in the world today. Along with more than one million new skin cancers diagnosed annually in the US (National Cancer Institute, www.cancer.gov), predicting and diagnosing skin disorders is important in its management. Current diagnostic methods rely mainly on visual observation and biopsy. However, detection methods that rely on visual observations are not necessarily effective in diagnosing a skin condition or disease, and do not detect a risk or disease until clinical manifestations occur. In addition, invasive methods such as biopsy increase the likelihood of infection as well as trauma to the test subject. In addition, the method should be performed by a physician in order to be safely performed, and usually does not provide a rich cell sample on the surface of the skin, which is generally a cell involved in the response.
Non-invasive methods for diagnosing and monitoring skin conditions and diseases therefore represent an important means for the management of patients and for evaluating the efficacy of existing and new treatments, skin care products and skin care regimens. In addition, the method can provide important information about specific genetic changes based on the skin condition of the test subject, as well as the genetic susceptibility of the test subject to the occurrence of skin disease. Identifying the genetic alteration may be important in identifying potential drug targets and preventative measures and determining whether a person actually responds to a particular therapeutic agent, skin care product or regimen. In addition, detection and diagnostic methods are important in assessing the safety of such treatments, products and measures.
In addition, it has been reported that the composition of skin material changes in various disease states as well as in local skin diseases. It is known that various substances such as lipids, structural proteins, inflammatory substances, nucleic acids, metabolites and the like are variously detected in the skin depending on the disease state. In addition to atopic dermatitis, melanoma and bacterial inflammation of the skin, biomarker analysis of skin has been performed in various diseases such as Alzheimer's disease, Parkinson's disease, breast cancer, cardiovascular disease, diabetes, drug addiction and the like. However, in most cases, a very aggressive skin biopsy is being used. In the noninvasive method, Iontophoresis, Microdialysis, Tape stripping, Ultrasound, and Microneedle are used, but they are very low in efficiency (Paliwal et al., 2013 Diagnostic opportunities based on biomarkers. Federation for Pharmaceutical Sciences 50: 546-556).
[Prior Patent Literature]
Korean Patent Publication No. 1020120006945
SUMMARY OF THE INVENTION The present invention has been made in view of the above problems, and it is an object of the present invention to provide a patch for use in early diagnosis of various diseases.
In order to achieve the above object, the present invention provides an application in which a microneedle is functionalized with a carbon nanotube field effect transistor (CNT-FET) coated with an aptamer to specifically react with a biomarker existing in the cell epilepsy in the skin It provides a diagnostic skin patch that enables real-time detection of electrical conductivity changes when combined with a tamer, amplifies it, and effectively transmits it to external devices to diagnose the disease early.
In an embodiment of the present invention, the biomarker is preferably a protein, a peptide, DNA, or RNA, but is not limited thereto.
In one embodiment of the present invention, the external device is a smart phone, a mobile phone, or a wireless communication device, but is not limited thereto.
Hereinafter, the present invention will be described.
Biomarkers of various diseases present in the epidermis layer below the stratum corneum can be detected in real time using a micro needle skin patch functionalized with an aptamer-coated carbon nanotube field effect transistor (CNT-FET) to be used for early diagnosis of various diseases It is an object of the invention.
It has been reported that the composition of skin material changes not only in local skin diseases but also in various disease states. It is known that various substances such as lipids, structural proteins, inflammatory substances, nucleic acids, metabolites and the like are variously detected in the skin depending on the disease state. In addition to atopic dermatitis, melanoma and bacterial inflammation of the skin, biomarker analysis of skin has been performed in various diseases such as Alzheimer's disease, Parkinson's disease, breast cancer, cardiovascular disease, diabetes, drug addiction and the like.
The present invention relates to a method for manufacturing a carbon nanotube field effect transistor (CNT-FET) coated with an aptamer-coated carbon nanotube field effect transistor (CNT-FET) having a length of about 200 μm that penetrates the stratum corneum of the skin and reaches the skin layer. It is a diagnostic skin patch that detects the change of electrical conductivity when a variety of biomarkers are combined with an aptamer that reacts specifically with it, amplifies it, and effectively transmits it to an external device so that various diseases can be diagnosed early. .
As can be seen from the present invention, the patch of the present invention senses the change in electrical conductivity when a variety of biomarkers existing in the intercellular epidermis bind to an aptamer specifically reacting thereto, So that various diseases can be diagnosed early.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a carbon nanotube extramammetic field effect transistor formed by attaching a linker to the surface of a carbon nanotube and fixing the extruder to the linker. In this case, CDI-tween 20 was used as a linker.
FIG. 2 is a graph showing the results obtained by attaching a carbon nanotube field effect transistor coated with various types of aptamers on a microneedle surface, amplifying a difference in electrical conductivity when an aptamer is combined with a specific biomarker, To monitor in real time.
FIG. 3 is a graph showing the relationship between the electric conductivity of a carbon nanotube field effect transistor coated with various types of aptamers on a channel inside a microneedle, To monitor in real time.
The present invention will now be described in more detail by way of non-limiting examples. The following examples are intended to illustrate the invention, but the scope of the invention is not to be construed as being limited by the following examples.
Example One. Aptamer Coated carbon nanotubes Field effect transistor making
A carbon nanotube sensor using aptamer as a molecular recognition material was first reported by the Korean Chemical Research Institute in 2005 (Journal of the American Chemical Society 127: 11906-11907). An electrode and an insulating layer were formed on the carbon nanotube grown directly on the substrate. Photolithography was used to form an electrode and an insulating layer. To detect a target substance on the surface of the carbon nanotube, CDI-Tween 20 was coated as a linker, (Figure 1).
Aptamer is a method of detecting a specific substance by using a three-dimensional structure of single strand DNA or RNA. It is similar to an antigen-antibody reaction, but its size is much smaller than that of antibody (10-15 nm) It has the advantage of being able to implement a field effect transistor much more effectively when coupled to a tube.
In addition, various types of substances can be detected simultaneously by attaching individual carbon nanotubes coated with various kinds of aptamers to various types of biomarkers (similar to a gene chip) by attaching them to a micro needle (Multiplexing).
Example 2. Microneedle tip on the surface Aptamer Attach coated carbon nanotubes
The carbon nanotubes coated with the aptamer were attached to the surface of each microneedle.
Example 3. Microneedle On the internal channel Aptamer Attach coated carbon nanotubes
Carbon nanotubes coated with an aptamer were attached to the channels inside each micro needle.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020150140741A KR20170041375A (en) | 2015-10-07 | 2015-10-07 | A Microneedle skin patch functionalized by Aptamer-coated carbon nanotube for early diagnosis of various diseases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020150140741A KR20170041375A (en) | 2015-10-07 | 2015-10-07 | A Microneedle skin patch functionalized by Aptamer-coated carbon nanotube for early diagnosis of various diseases |
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| Publication Number | Publication Date |
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| KR20170041375A true KR20170041375A (en) | 2017-04-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| KR1020150140741A KR20170041375A (en) | 2015-10-07 | 2015-10-07 | A Microneedle skin patch functionalized by Aptamer-coated carbon nanotube for early diagnosis of various diseases |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020069567A1 (en) | 2018-10-02 | 2020-04-09 | WearOptimo Pty Ltd | Electrode arrangement |
| WO2020069565A1 (en) | 2018-10-02 | 2020-04-09 | WearOptimo Pty Ltd | Measurement system |
| US11891652B2 (en) | 2017-11-24 | 2024-02-06 | Queen Mary University Of London | Biosensor device and assembly methods |
-
2015
- 2015-10-07 KR KR1020150140741A patent/KR20170041375A/en unknown
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11891652B2 (en) | 2017-11-24 | 2024-02-06 | Queen Mary University Of London | Biosensor device and assembly methods |
| WO2020069567A1 (en) | 2018-10-02 | 2020-04-09 | WearOptimo Pty Ltd | Electrode arrangement |
| WO2020069565A1 (en) | 2018-10-02 | 2020-04-09 | WearOptimo Pty Ltd | Measurement system |
| US12048558B2 (en) | 2018-10-02 | 2024-07-30 | WearOptimo Pty Ltd | System for determining fluid level in a biological subject |
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