KR20170083172A

KR20170083172A - 3'UTR engineering to improve soluble expression of foreign proteins in microbial cells

Info

Publication number: KR20170083172A
Application number: KR1020160001809A
Authority: KR
Inventors: 박진병; 송지원
Original assignee: 이화여자대학교 산학협력단
Priority date: 2016-01-07
Filing date: 2016-01-07
Publication date: 2017-07-18
Also published as: KR101769231B1

Abstract

The present invention relates to a method for increasing the water-soluble expression ratio relative to the total expression level of a target protein, an expression vector for increasing the water-soluble expression ratio to the total expression level of the target protein, and an aqueous expression ratio relative to the total expression level of the target protein The present invention relates to a transformant which can increase the transfection efficiency.
The activity of the enzyme can be controlled by increasing the expression rate of the water-soluble protein of the present invention, and the productivity of various chemical substances can be increased by using whole cell biotransformation.

Description

(3'UTR engineering to improve expression of foreign proteins in microbial cells via 3'UTR engineering)

The present invention relates to a method for increasing the water-soluble expression ratio relative to the total expression level of a target protein, an expression vector for increasing the water-soluble expression ratio relative to the total expression level of the target protein, and an expression vector, To a transformant capable of increasing the ratio.

Synthetic biology and system biology enable the development of a variety of chemicals through whole-cell biocatalysts and microbial fermentation. Large and complex metabolites (eg, polyphenols, carotenoids, terpenoids, plant oxylipins) as well as small substances can also be synthesized with the help of system metabolic engineering.

However, the chemical is not very productive due to many factors such as low expression level of the enzyme in the microbial catalyst. Above all, oxygenated enzymes (P450 monooxygenases (BVMO) and Bayer-Villiger monooxygneases (BVMO) are one of the key enzymes for the functionalization of oxidized hydrocarbons as well as renewable biomass, and the production of large and complex metabolites in fatty acids , But overexpression of functional forms (i. E., Water soluble forms) of these enzymes in bacterial cells is difficult.

Functional expression of heterologous proteins such as oxygenated enzymes in bacterial cells can be improved by a variety of methods. (Chalmers et al., 1990, Appl. Environ. Microbiol., 56, 104-111), as well as the concentration of the inducer, as well as the promoter, ribosome binding site, 5'- (5'-UTR), and codon usage, have also been extensively studied in order to enhance the aqueous expression of enzymes and proteins. In addition, molecular chaperons have been introduced, and water-soluble peptides and Protein expression and other protein engineering (e. G., Inducible evolution) often allow functional expression of external proteins and enzymes in bacterial cells (Ario de Marco et al., 2007, BMC Biotechnology, 7:32).

The 3'UTR is known to serve as a transcription terminator that contributes to the stability of mRNA in prokaryotes. Thereby, it is used to increase the stability of the transcriptionally activated mRNA by inserting a specific sequence (for example, REP sequence) as the 3'UTR. For example, it has been reported that the REP sequence as a 3'UTR is inserted to increase the stability of mRNA and thereby significantly increase the expression level of MalE protein in E. coli. However, studies have not yet been conducted to insert a nucleotide sequence containing an RNase E cleavage site into the 3 'UTR to decrease mRNA stability and thereby increase the functional expression (soluble expression) of the protein.

Under these circumstances, the present inventors have made extensive efforts to develop a new method for increasing the water solubility of the enzyme in E. coli. As a result, it has been found that a nucleotide sequence having an RNase E cleavage site in the 3 'terminal UTR of a gene encoding a desired water- To obtain and express the polynucleotide prepared by inserting the polynucleotide of the present invention into the expression level of the desired protein, thereby completing the present invention.

It is an object of the present invention to provide a method for increasing the water-soluble expression ratio to the total expression level of a desired protein.

Another object of the present invention is to provide an expression vector for increasing the water-soluble expression level relative to the total expression level of the desired protein.

It is another object of the present invention to provide a transformant which can introduce the expression vector and increase the water-soluble expression ratio relative to the total expression level of the desired protein.

The present inventors have conducted various studies to develop a new method for increasing the expression level of water-soluble proteins for the purpose of improving the activity of enzymes used in industry. In the course of carrying out various studies, it has been found that by inserting the RNase E cleavage site into the 3'UTR, To increase the rate of expression of soluble proteins. That is, in the case where Pseudomonas fluorescens ( Pseudomonas fluorescens) 　 fluorescens BmoF1, BVMO (Bayer-Villiger monooxygenase) derived from DSM50106 or Rhodococcus jostii ) In the overexpression of MO16 as a BVMO derived from RHA1 by a recombinant method, a nucleotide sequence (for example, a CAT sequence or the like) including an RNase E cleavage site which reduces the stability of mRNA is added to the 3 'end of the gene encoding the enzyme, The polynucleotide having the inserted UTR-linked polynucleotide was obtained. When the polynucleotide was expressed, the expression ratio of the water-soluble protein form to the total expression level of BmoF1 or MO16 was increased. Techniques for increasing the protein expression level by increasing the stability of mRNA by binding to the UTR at the 5 'or 3' end of the desired gene are known, but the RNase E cleavage site provided by the present invention is not limited to the stability of the mRNA To increase the expression rate of a water-soluble protein has not been known at all and has been developed for the first time by the present inventors.

To achieve the above object, one aspect of the present invention is a method for producing a water soluble protein comprising the steps of: (a) obtaining a polynucleotide to which an UTR (untranslated region) is bound to increase the mRNA stability at the 3 ' ; And (b) expressing the polynucleotide, wherein a nucleotide sequence containing an RNase E cleavage site, which lowers the stability of the mRNA, is inserted in the base sequence of the UTR, Thereby increasing the water-soluble expression ratio relative to the expression level.

Generally, when the water-soluble protein is expressed, the stability of the mRNA transcribed from the polynucleotide is improved by binding the UTR to the 5 'or 3' end of the polynucleotide encoding the protein, thereby increasing the synthesis level of the polypeptide On the other hand, this leads to an increase in the local concentration of the polypeptide before folding of the protein occurs. As the local concentration increases, the polypeptides aggregate with each other to form an inclusion body. As a result, the level of the protein expressed as a water-soluble form produced by normal folding is reduced. In order to solve the above problem, the present invention inserts a base sequence including an RNase E cleavage site into a 3'UTR to reduce mRNA stability and decrease the number of synthesized polypeptides, thereby reducing local concentration, Increased levels of water - soluble proteins were produced by increasing the expression level of water - soluble proteins.

In the present invention, the nucleotide sequence containing the RNase E cleavage site is inserted into the 3'UTR to reduce the mRNA stability, thereby controlling the amount of the protein expressed, thereby reducing the amount of protein expression by regulating the induction degree of the promoter, (5 'UTR or ribosome binding site (RBS)) to decrease the rate of translation at the mRNA level.

As a method for promoting the production of a water-soluble protein in E. coli, a recombinant protein expression vector may be constructed by expressing a desired protein with an amino acid having a high water-solubility, or by expressing it in a periplasm in connection with a signal sequence secretion), and a method of simultaneously expressing chaperones involved in protein folding are known. It is generally known that a low culture temperature is generally advantageous for the water-soluble expression of proteins. (Pilon et al., 1996, Biotechnol. Prog., 12, 331-337) used 15 amino acid sequences of ubiquitin as a fusion partner with 17 amino acid sequences to form 15 peptides ) Were expressed, and they were all expressed as water-soluble by applying heat shock. However, in comparison with the above method, the present invention is characterized in that a specific base sequence including a plurality of RNase E cleavage sites is inserted into the 3 'UTR to decrease the mRNA stability, thereby inducing normal folding of the polypeptide to increase the expression of the water- Respectively.

The term "desired water-soluble protein " as used herein refers to a protein that expresses both water-insoluble and water-soluble proteins, and particularly refers to a protein having significantly less water-soluble expression compared to the expression level when the protein is over-expressed. In the present invention, the desired water-soluble protein may be used in combination with the desired protein in the same sense.

In the present invention, the desired water-soluble protein may be a protein having an aqueous expression ratio of 50% or less with respect to the total expression level of the protein by over-expressing the protein when the over-expressed protein co-aggregates in the cell to form an inclusion body. Pseudomonas fluorescein sense (Pseudomonas 　 fluorescens derived from DSM 50106 or Rhodococcus jostii , RHA1 may be derived BVMO (Bayer-Villiger monooxygenase), and more particularly, Pseudomonas fluorescein sense (Pseudomonas 　 fluorescens DSM50106 derived BVMO BmoF1 or Rhodococcus jostii ) MO16, which is a BVMO derived from RHA1.

As used herein, the term "water soluble protein" refers to a protein that is transcribed and decoded by expression of the gene of interest, and then expressed in water by folding. In addition, the expression level of the water-soluble protein may be used in combination with the water-soluble expression of the protein in the same sense.

As used herein, the term "untranslated region " (UTR) is also referred to as an uninterpreted region, and refers to a portion of the mRNA that does not become a template for a protein gene, 3 'UTR (3' uninterpreted site), 3 'UTR means a transcription terminator that contributes to the stability of the mRNA in prokaryotes. For example, the REP sequence is inserted to increase the stability of transcriptionally activated mRNA. In the present invention, 3'UTR was used in order to increase the water-soluble expression ratio relative to the total expression level of the desired protein.

As used herein, the term "RNase E cleavage site" means a site where cleavage occurs by RNase E involved in the treatment and degradation of RNA in bacterial cells, and mainly cleavage occurs in the AU-rich region of a single strand. The RNase E cleavage site is present in the 3'UTR of BmoF1 or MO16, which is the desired water-soluble protein of the present invention. In the 3'UTR of BmoF1, 5 RNase E cleavage sites are included in the 5 ' have. In the present invention, the hilD nucleotide sequence or the CAT nucleotide sequence was used to insert the RNase E cleavage site into the 3 'UTR. Also, as the number of RNase E cleavage sites increases, the mRNA stability decreases, the expression level of the water soluble protein increases, and the biotransformation activity increases.

In the present invention, the RNase E cleavage site is inserted into the UTR and used as a means for lowering the stability of the mRNA. Specifically, the number of the RNase E cleavage sites inserted in the 3'UTR is 10 to 100 And more specifically, 12 to 30 can be inserted. More specifically, the number of RNase E cleavage sites included in the hilD base sequence, which is a model system, may be 12 to 16, and the number of RNase E cleavage sites included in the CAT nucleotide sequence as a model system is 13 to 30 number and, most specifically hilD nucleotide sequence is 14, the CAT257 base sequence comprising 257 nucleotides has CAT357 base sequence comprising 15, 357 nucleotides is CAT557 base sequence comprising 18, 557 nucleotides 26 The CAT657 base sequence may contain 28 RNase E cleavage sites.

In order to amplify a CAT nucleotide sequence fragment having a different number of RNase E cleavage sites, a CAT657 sequence containing 657 nucleotides was amplified by PCR using CAT_F and CAT_R primers corresponding to SEQ ID NO: 7 and SEQ ID NO: 8, respectively. In addition, a CAT257 sequence containing 257 nucleotides was amplified using CAT_F and CAT257_R primers corresponding to SEQ ID NO: 7 and SEQ ID NO: 9, 357 nucleotides were amplified using CAT_F and CAT357_R primers corresponding to SEQ ID NO: 7 and SEQ ID NO: Was obtained by amplifying the CAT557 sequence containing 557 nucleotides through PCR using the CAT_F and CAT557_R primers corresponding to SEQ ID NO: 7 and SEQ ID NO: 11, respectively.

In addition, as the number of RNase E cleavage sites is adjusted, the water-soluble expression ratio relative to the total expression level of the desired protein can be changed. Specifically, in the case of expression of BmoF1, as the number of RNase E cleavage sites increases, But may be to increase the rate of water-soluble expression relative to the level of expression.

In one embodiment of the present invention, the CAT nucleotide sequences (CAT257, CAT357, CAT557, CAT657) containing the number of other RNase E cleavage sites were inserted into the 3'UTR of BmoF1, a BVMO derived from Pseudomonas fluorescens DSM50106, E. coli BL21 (DE3) pET-BmoF1-3'UTR _CAT257 , pET-BmoF1-3'UTR _CAT357 , pET-BmoF1-3'UTR _CAT557 and pET-BmoF1-3'UTR _CAT657 were _prepared and the number of RNase E cleavage sites As a result of confirming the level of BmoF1-3'UTR _CAT mRNA, it was confirmed that the level of BmoF1-3'UTR _CAT mRNA decreased as the number of RNase E cleavage sites present in the nucleotide of the CAT nucleotide sequence increased. In addition, RNase E BmoF1-3'UTR _CAT results confirm the protein expression level, the more the increase in the number of RNase E cleavage site BmoF1-3'UTR Total _CAT protein expression levels according to the number of the cut is however reduced, the water-soluble BmoF1-3'UTR _CAT Protein expression levels were increased. This indicates that the number of RNase E cleavage sites affects mRNA stability and water-soluble protein expression level (FIG. 5).

The nucleotide sequence including the RNase E cleavage site is not particularly limited as long as it contains an RNase E cleavage site, and all or part of the hilD nucleotide sequence or the CAT nucleotide sequence may be used. (CAT257, CAT357, CAT557) are represented by SEQ ID NOs: 18 to 20, and the entire CAT nucleotide sequence (CAT657) is represented by SEQ ID NO: 21 to SEQ ID NO: Lt; / RTI >

The hilD nucleotide sequence contains 14 RNase E cleavage sites as a transcriptional regulator of the SPI1 gene. In the present invention, the RNase E cleavage site contained in the hilD nucleotide sequence is inserted into the 3'-terminal UTR of the desired water-soluble protein, And to increase the expression level of the water-soluble protein relative to the total expression level of the desired protein.

In the present invention, the hilD nucleotide sequence (SEQ ID NO: 17) was amplified by PCR using the hilD_F and hilD_R primers corresponding to SEQ ID NO: 1 and SEQ ID NO: 2, respectively, in order to amplify the hilD nucleotide sequence fragment.

In one embodiment of the invention the nucleotide sequence of hilD base sequence comprising RNase E cleavage site Pseudomonas fluorescein sense (Pseudomonas 　 fluorescens) DSM50106 derived BVMO of the recombinant E. coli BL21 (DE3) into the 3'UTR of BmoF1 pET-BmoF1-3'UTR _hilD RNase E has the nucleotide sequence of Pseudomonas hilD base sequence comprising a cleavage site fluorenyl sense (Pseudomonas fluorescens ) Compared with BL21 (DE3) pET-BmoF1-3'UTR _{native which} was not inserted in the 3'UTR of BmoF1 derived from DSM50106 derived BVMO, BmoF1-3'UTR _hilD mRNA level was lowered and RNase The insertion of the hilD nucleotide sequence containing the E-cleavage site influenced the stability of the desired water-soluble protein BmoF1 (Fig. 3A).

According to another embodiment of the invention the nucleotide sequence of hilD base sequence comprising RNase E cleavage site Pseudomonas fluorescein sense (Pseudomonas 　 fluorescens) DSM50106 derived BVMO of the recombinant E. coli BL21 (DE3) into the 3'UTR of BmoF1 pET-BmoF1-3'UTR _hilD RNase E has the nucleotide sequence of Pseudomonas hilD base sequence comprising a cleavage site fluorenyl sense (Pseudomonas fluorescens ) Compared with BL21 (DE3) pET-BmoF1-3'UTR _{native which} was not inserted in the 3'UTR of BmoF1, a BmOF1-3'UTR _hilD total protein expression level, but BmoF1-3'UTR _hilD It was found that the expression level of the water-soluble protein BmoF1, which is an intended water-soluble protein, can be improved by inserting the hilD nucleotide sequence containing the RNase E cleavage site into the 3'UTR of Escherichia coli (FIG. 3B).

The CAT (chloramphenicol acetyltransferase) base sequence refers to a sequence encoding an enzyme that acetylates chloramphenicol by an antibiotic substance isolated from E. coli. The above-mentioned chloramphenicol selectively binds to the 50S ribosome to inhibit the peptide transfer reaction, thereby inhibiting protein synthesis of the prokaryote. The acetylated chloramphenicol by the CAT enzyme can not bind to the 50S ribosome, and the protein synthesis inhibitory activity of the chloramphenicol is decreased do. For this reason, the CAT sequence is mainly used as a reporter gene used for detecting the expression activity by gene introduction. However, in the present invention, the CAT nucleotide sequence is intended to insert the RNase E cleavage site contained in the CAT nucleotide sequence into the 3'-terminal UTR of the desired water-soluble protein to reduce the mRNA stability and increase the water-soluble expression level of the desired protein Respectively.

In the present invention, as the length of the CAT nucleotide sequence increased, the number of RNase E cleavage sites contained in the nucleotide sequence increased.

In one embodiment of the invention the sequence of the base sequence CAT, which contains the RNase E cleavage site Pseudomonas fluorescein sense (Pseudomonas 　 fluorescens) DSM50106 derived BVMO the BmoF1 the nucleotide sequence of SEQ ID NO: CAT Pseudomonas fluorescein sense that the recombinant E. coli BL21 (DE3) pET-BmoF1-3'UTR _CAT inserted into the 3'UTR contains a cleavage site for RNase E (Pseudomonas fluorescens) Compared with recombinant Escherichia coli BL21 (DE3) pET-BmoF1-3'UTR _{native which} was not inserted into the 3'UTR of BmoF1 derived from DSM50106-derived BVMO, BmoF1-3'UTR _CAT mRNA level was reduced by about half, and RNase E Insertion of the CAT nucleotide sequence containing the cleavage site influenced mRNA stability (Fig. 5A).

According to another embodiment of the invention the CAT nucleotide sequence of the nucleotide sequence comprising the RNase E cleavage site Pseudomonas fluorescein sense (Pseudomonas 　 fluorescens) DSM50106 base sequence of base sequence CAT resulting BVMO of the recombinant E. coli BL21 (DE3) pET-BmoF1-3'UTR _CAT inserted into the 3'UTR of BmoF1 comprises an RNase E cleavage sites are Pseudomonas fluorescein sense (Pseudomonas fluorescens ) Compared to recombinant E. coli BL21 (DE3) pET-BmoF1-3'UTR _{native which} was not inserted into the 3'UTR of BmoF1, a BmOF1-3'UTR _CAT total protein expression level of DSM50106 derived BVMO, BmoF1-3 ' It was confirmed that the expression level of the UTR _CAT water-soluble protein was increased, and it was found that the CAT nucleotide sequence containing the RNase E cleavage site was inserted into the 3'UTR to improve the water-soluble protein expression level of BmoF1 B and C).

As used herein, the term "BVMO (Baeyer-Villiger monooxygenase)" refers to a kind of monooxygenase which is an enzyme capable of catalyzing various oxidation reactions including Baeyer-Villiger oxidation reaction in which ketones are oxidized to produce lactones or ester compounds it means. For the purpose of the present invention, the BVMO (Baeyer-Villiger monooxygenase (BVMO)), which is expressed in a transformant and exhibits an activity of catalyzing a reaction for producing a fatty acid derivative into which an ester group is introduced from a chitosan fatty acid ) Is not particularly limited, but specifically, a strain of Pseudomonas sp. sp . ), Rhodococcus sp . Brevibacterium sp . ), Eggplant komano sp (Comanonas sp.), Acinetobacter sp (Acinetobacter sp . ), Arthrobacter sp . , Brachymonas sp . sp . ) And the like can be a BVMO (Baeyer-Villiger monooxygenase) derived from microorganisms such as, and more specifically Pseudomonas fluorescein sense (Pseudomonas 　 fluorescens DSM 50106 or Rhodococcus jostii ) Can be BVMO (Baeyer-Villiger monooxygenase) derived from RHA1.

In the present invention, Pseudomonas fluorescein sense (Pseudomonas 　 fluorescens) DSM50106 to amplify the resulting BVMO BmoF1 the gene, by performing PCR with the primer of SEQ ID NO: 3 and SEQ ID NO: 4 was amplified BmoF1 gene.

By inserting the RNase E cleavage site present in the CAT nucleotide sequence in the present invention Pseudomonas fluorescein sense (Pseudomonas 　 fluorescens DSM50106-derived BmOF1 gene, the BmoF1 protein was found to increase the water-soluble protein expression of the BmOF1 protein. Therefore, it was confirmed that the BmOF1 protein had a homology of about 23.3% (Fig. 8) Rhodococcus jostii ) The expression level of the MO16 protein was confirmed by inserting the RNase E cleavage site in the CAT nucleotide sequence at the 3'UTR end of the MO16 gene, BVMO (Baeyer-Villiger monooxygenase) derived from RHA1.

In the present invention, in order to amplify the MO16 gene, PCR was performed with MO16_F and MO16_R primers corresponding to SEQ ID NO: 12 and SEQ ID NO: 13 to amplify the MO16 gene.

In order to amplify the CAT nucleotide sequence containing the RNase E cleavage site to be inserted into the 3'UTR of the MO16, MO16_CAT_F and MO16_CAT_R primers corresponding to SEQ ID NO: 14 and SEQ ID NO: 15 were used to amplify the CAT nucleotide sequence of MO16_CAT657 containing 657 nucleotides Was obtained by amplifying the base sequence using PCR, and was obtained by amplifying the MO16_CAT257 nucleotide sequence containing 257 nucleotides using the MO16_CAT_F and MO16_CAT257_R primers corresponding to SEQ ID NO: 14 and SEQ ID NO: 16, respectively.

In one embodiment of the present invention, the CAT nucleotide sequences (MO16_CAT257 and MO16_CAT657) including the RNase E cleavage site are introduced into Rhodococcus jostii ) RHA1 derived BVMO of the recombinant E. coli BL21 into the MO16 gene 3'UTR (DE3) pET22b-MO16-3'UTR _CAT257 and recombinant E. coli BL21 (DE3) pET22b-MO16-3'UTR _CAT657 of MO16-3'UTR _CAT257 and MO16 -3'UTR _CAT657 protein expression levels were compared with the recombinant E. coli BL21 (DE3) pET22b-MO16-3'UTR _native MO16-3'UTR _native protein level of expression, recombinant E. coli BL21 (DE3) pET22b-MO16-3 ' no protein expression was not at UTR _CAT657, recombinant E. coli BL21 (DE3) from pET22b-MO16-3'UTR _CAT257 MO16-3'UTR _CAT257 soluble protein expression the recombinant E. coli BL21 (DE3) pET22b-MO16-3'UTR _CAT657 of MO16 -3 ' _UTR CAT257. _Therefore , the number of RNase E cleavage sites present in the CAT nucleotide sequence in the MO16 enzyme can be adjusted to improve the expression of water-soluble proteins and the water-soluble expression of the MO16 protein is more sensitive than the aqueous expression of BmoF1 (Fig. 9).

In another embodiment of the present invention, a nucleic acid encoding a water-soluble protein is provided, wherein a multicloning site capable of introducing the UTR-linked polynucleotide, which can increase the mRNA stability, site and an UTR in which a base sequence containing an RNase E cleavage site, which binds to the 3 'end of the multiple cloning site and reduces the stability of the mRNA, is increased in proportion to the total expression level of the desired protein Lt; / RTI > expression vector.

The term "expression vector" as used herein refers to a gene construct comprising a gene insert that is capable of expressing a desired protein in an appropriate host cell, and an essential regulatory element operably linked to the expression of said gene insert . The expression vector includes expression control elements such as an initiation codon, a termination codon, a promoter, an operator, etc. The initiation codon and termination codon are generally regarded as part of the nucleotide sequence encoding the polypeptide, and when the gene product is administered, And must be in coding sequence and in frame. The promoter of the vector may be constitutive or inducible.

The expression vector is not particularly limited as long as it is capable of expressing a UTR-linked polynucleotide into which a base sequence including an RNase E cleavage site is inserted at the 3 'end of a gene encoding a desired water-soluble protein. Such as human, monkey, rabbit, rat, hamster, mouse, etc.), plant cells, yeast cells, insect cells or bacterial cells (for example, Escherichia coli) And / or may be a vector that is expressible, specifically operably linked to a suitable promoter so that the polynucleotide can be expressed in a host cell, and may be a vector comprising at least one selectable marker, Specifically, commercially available plasmids (pUC18, pBAD, pIDTSAMRT-AMP, etc.), E. coli-derived plasmids (pYG601BR322, pBR3 Derived plasmids (pUB110 and pTP5), yeast-derived plasmids (YEp13, YEp24 and YCp50), lambda-phage (Charon4A, Charon21A, EMBL3, EMBL4, lambda gt10, pUC118 and pUC119), Bacillus subtilis- lambda gt11 and lambda ZAP), retrovirus, adenovirus, vaccinia virus, baculovirus, and the like. Since the amount of expression of the protein and the expression of the expression vector are different depending on the host cell, it is preferable to select and use the host cell most suitable for the purpose.

In another aspect of the present invention, there is provided a transformant, wherein the expression vector is introduced to increase the water-soluble expression ratio relative to the total expression level of a desired protein.

As used herein, the term "transformant" means a cell or microorganism that has been mutated to express the desired protein after the polynucleotide encoding the desired protein has been introduced into the host using a vector . At this time, the polynucleotide introduced into the host cell may be in any form as long as it can be introduced into the host cell and expressed.

The transformant provided in the present invention is a transformant having an RNase E cleavage site at the 3 'end of a gene encoding MO16 which is BVMF1 or Rhodococcus jostii RHA1 derived BVMO derived from Pseudomonas fluorescens DSM50106 BVMO May be prepared by introducing into a host cell an expression vector capable of expressing a UTR-linked polynucleotide into which the nucleotide sequence containing the UTR is inserted. In this case, the host cell that can be used is not particularly limited. As an example, a culturable single-celled prokaryotic or eukaryotic cell suitable for application to the bioconversion process may be used, and E. coli, yeast, etc., As another example, Escherichia coli BL21 (DE3) cells can be used.

In addition, the transformation can be carried out by various methods. As long as a transformant exhibiting an effect of improving the expression level of a water-soluble protein can be produced, the method can be carried out by any of CaCl ₂ precipitation method, CaCl ₂ ₂ precipitation method using a reducing material called DMSO (dimethyl sulfoxide), an electroporation method, an electroporation method, a calcium phosphate precipitation method, a protoplast fusion method, an agitation method using silicon carbide fiber, an agrobacterium-mediated trait A transformation method using PEG, a dextran sulfate, a lipofectamine, and a dry / suppression-mediated transformation method.

Through the culturing process of the transformants provided in the present invention, the water-soluble expression ratio relative to the total expression level of the desired protein can be increased.

The term "cultivation" of the present invention means a process of growing microorganisms under an appropriately artificially controlled environmental condition.

In the present invention, the culturing can be understood as a process for growing the transformant to carry out a biotransformation process. Specifically, the culturing can be performed in a batch or continuous manner through a batch process, an implantation batch process, .

The culture medium used for the above cultivation should meet the requirements of a specific strain in an appropriate manner while controlling the temperature, pH and the like under aerobic conditions in an ordinary medium containing an appropriate carbon source, nitrogen source, amino acid, vitamin, and the like. Carbon sources that may be used include sugars, fats, fatty acids and glycerol such as glucose, sucrose, and lactose. These materials may be used individually or as a mixture. Nitrogen sources that may be used include inorganic sources such as ammonia, ammonium sulfate, ammonium chloride, ammonium phosphate; Amino acids such as glutamic acid, and organic peptides such as peptone, meat extract, yeast extract, malt extract, corn steep liquor, and casein hydrolyzate. These nitrogen sources may be used alone or in combination.

The method may further comprise the step of injecting oxygen or an oxygen-containing gas (e.g., air) into the culture to maintain the aerobic state during the culture, and the temperature of the culture may be in the range of 27 to 37 Lt; 0 > C, and the incubation time can be 10 to 100 hours.

The method of increasing the water-soluble expression ratio relative to the expression level of the target protein provided by the present invention can be applied to various industrial fields using water-soluble proteins. If the yield of water-soluble proteins used in the industrial field is increased, It can reduce costs and improve industrial competitiveness.

An example of an application method for such an industrial field is a biotransformation process using a transforming microorganism disclosed in Korean Patent No. 1500827 or Korean Patent No. 1534910.

In one embodiment of the present invention, the ester product of BmoF1 in recombinant Escherichia coli BL21 (DE3) expressing pCOLA-ADH and pET22b-BmoF1-3'UTR _hilD through whole cell biotransformation is pCOLA-ADH and pET22b-BmoF1-3 'UTR _Native Recombinant E. coli BL21 (DE3). It was found that insertion of the hilD nucleotide sequence containing the RNase E cleavage site at the 3'UTR also affected the water-soluble expression and bioconversion activity of the BmoF1 enzyme (Fig. 3C).

In another embodiment of the present invention, as the number of RNase E cleavage sites increases in recombinant E. coli BL21 (DE3) expressing pCOLA-ADH and pET22b-BmoF1-3'UTR _CAT variants through whole cell biotransformation, , Production rate and ester product increased, indicating that the number of RNase E cleavage sites also affected the water-soluble expression of BmoF1 enzyme and the bioconversion activity (FIGS. 6 and 7).

In another embodiment of the present invention, the catalytic activity of the recombinant Escherichia coli BL21 (DE3) expressing pET22b-MO16-3'UTR _CAT257 was compared with the activity of pET22b-MO16-3'UTR _native (RNase E) cleavage site in the CAT nucleotide sequence and the number of RNase E cleavage sites in the microorganism was confirmed to be 35% higher than that of the recombinant E. coli BL21 (DE3) Expression and bioconversion activity (Fig. 11A and B).

As used herein, the term " bioconversion activity "refers to a concept that includes the rate of bioconversion and the rate of yield or yield of a reactant that occurs upon conversion of whole cell biology.

The activity of the enzyme can be controlled by increasing the expression rate of the water-soluble protein of the present invention, and the productivity of various chemical substances can be increased by using whole cell biotransformation.

Figure 1 shows SDS-PAGE analysis of cell extracts isolated from the culture medium of recombinant E. coli BL21 (DE3) expressing pCOLADuet-ADH and pET22b-BmoF1. Lane M is a marker, Lane S is a soluble fraction of recombinant E. coli BL21 (DE3) expressing pCOLADuet-ADH and pET22b-BmoF1, Lane I is a water-insoluble fraction of recombinant E. coli BL21 (DE3) expressing pCOLADuet-ADH and pET22b- Fractions.
FIG. 2 is a graph showing the activity of Salmonella enterica 　 enterica ) and a CAT657 nucleotide sequence containing 657 nucleotides.
Figure 2 A shows Salmonella enterica 　 is a diagram showing the base sequence of hilD enterica).
FIG. 2B is a diagram showing a CAT657 nucleotide sequence containing 657 nucleotides. The CAT257 nucleotide sequence containing 257 nucleotides in the CAT 657 nucleotide is yellow, the CAT357 nucleotide sequence containing 357 nucleotides is yellow + gray, and the CAT557 nucleotide sequence containing 557 nucleotides is yellow + gray + dark green. Respectively. RNase E cleavage sites were shown in red.
Fig. 3 is a diagram showing mRNA level and protein expression level of BmoF1 in Escherichia coli and bioconversion of ricinoleic acid using BmoF1 enzyme.
Figure 3 A is a Bmof1 of recombinant E. coli BL21 (DE3) pET22b-BmoF1-3'UTR _native and pET22b-BmoF1-3'UTR _hilD recombinant E. coli _{BL21 (DE3) - 3'UTR native (} lane 1) and Bmof1 - 3 ' _Lt; RTI ID ₌ 0.0 > UTR _hilD (lane 2). & _{Lt; /} RTI > Recombinant Escherichia coli BL21 (DE3) pET22b-BmoF1-3'UTR _native and recombinant Escherichia coli BL21 (DE3) pET22b-BmoF1-3'UTR _hilD And the level of ihfB mRNA was used as a control.
B of FIG. 3 shows the results obtained from the wild-type Escherichia coli BL21 (DE3) (lane WS and WI), recombinant Escherichia coli BL21 (DE3) pET22b-BmoF1-3'UTR _native (lane 1 and 2), and recombinant Escherichia coli BL21 (DE3) pET22b- SDS-PAGE analysis of 3'UTR _hilD (lane 3 and 4) cell extracts. Lane M is a marker, Lane WS, 1 and 3 are water-soluble fractions, Lane WI, 2 and 4 are water-insoluble fractions.
Figure 3C shows recombinant E. coli BL21 (DE3) expressing pCOLADuet-ADH and pET22b-BmoF1-3'UTR _native (left panel), pCOLADuet-ADH and pET22b-BmoF1-3'UTR _hilD expressing recombinant Escherichia coli BL21 DE3) (lower panel), showing the conversion of whole cell biosyntheses from ricinoleic acid to an ester. The experiment was performed three times and the error bars were expressed as standard deviations. The symbols are respectively ricinoleic acid (?), 12-ketooleic acid (?), Ester (11) and 11-hydroxyundec-9-enoic acid ).
FIG. 4 is a diagram showing the number of RNase E cleavage sites of a 3'UTR _CAT mutant inserted with a CAT nucleotide sequence containing 257 to 657 nucleotides. The BmoF1 gene contains 5 RNase E, 15 CAT257, 18 CAT357, 26 CAT557, and CAT657 (the same as the CAT sequence without partial deletion). In addition, there are 19 hilD sequences and 10 RNase E cleavage sites in the existing UTR of the MO16 gene.
5 is an analysis of BmoF1-3'UTR _CAT mRNA level and protein expression level in recombinant E. coli BL21 (DE3) pET22b-BmoF1-3'UTR _CAT variants (CAT257, CAT357, CAT557, CAT657).
FIG. 5A is a graph showing the level of BmoF1-3'UTR _CAT mRNA in the recombinant E. coli BL21 (DE3) pET22b-BmoF1-3'UTR _CAT mutant. Samples recombinant E. coli _{BL21 (DE3) pET22b-BmoF1-3'UTR native} (lane 1), recombinant E. coli _{BL21 (DE3) pET22b-BmoF1-3'UTR CAT} -257 (lane 2), recombinant E. coli BL21 (DE3) pET22b- BmoF1-3'UTR _CAT _-357 (lane 3), recombinant E. coli _{BL21 (DE3) pET22b-BmoF1-3'UTR CAT} -557 (lane 4) , and recombinant E. coli _{BL21 (DE3) pET22b-BmoF1-3'UTR CAT} -657 (lane 5). The mRNA level of ihfB was used as a control.
Figure 5B shows SDS-PAGE (upper panel) and Western blot (lower panel) analysis of the BmoF1-3'UTR _CAT water soluble fraction in recombinant E. coli BL21 (DE3) pET22b-BmoF1-3'UTR _CAT variants to be. Samples recombinant E. coli _{BL21 (DE3) pET22b-BmoF1-3'UTR native} (lane 1), E. coli _{BL21 (DE3) pET22b-BmoF1-3'UTR CAT} -257 (lane 2), recombinant E. coli BL21 (DE3) pET22b-BmoF1 3'UTR _CAT- ₃₅₇ (lane 3), recombinant Escherichia coli BL21 (DE3) pET22b-BmoF1-3'UTR _CAT-557 (lane 4), and recombinant Escherichia coli BL21 (DE3) pET22b-BmoF1-3'UTR _CAT-657 (lane 5), and Lane M represents a marker.
FIG. 5C is an SDS-PAGE (upper panel) analysis of the BmoF1-3'UTR _CAT insoluble fraction in recombinant E. coli BL21 (DE3) pET22b-BmoF1-3'UTR _CAT variants. Samples recombinant E. coli _{BL21 (DE3) pET22b-BmoF1-3'UTR native} (lane 1), recombinant E. coli _{BL21 (DE3) pET22b-BmoF1-3'UTR CAT} -257 (lane 2), E. coli BL21 (DE3) pET22b-BmoF1 _{-3'UTR CAT-357 (lane 3)} , E. coli _{BL21 (DE3) pET22b-BmoF1-3'UTR CAT} -557 (lane 4), and E. coli _{BL21 (DE3) pET22b-BmoF1-3'UTR CAT} -657 (lane 5) was used, and Lane M represents a marker.
6 is a diagram showing biotransformation from ricinoleic acid to an ester using a mutant of recombinant E. coli BL21 (DE3) pET22b-BmoF1-3'UTR _CAT .
Figure 6 A to C of the recombinant E. coli _{BL21 (DE3) pET22b-BmoF1-3'UTR native} , Figure 6 is a recombinant E. coli B _{BL21 (DE3) pET22b-BmoF1-3'UTR CAT} -257, Figure 6 is a recombinant E. coli BL21 (DE3) pET22b-BmoF1-3'UTR _CAT- ₃₅₇ , FIG. 6D shows recombinant E. coli BL21 (DE3) pET22b-BmoF1-3'UTR _CAT-557 and FIG. 6E shows recombinant E. coli BL21 (DE3) pET22b- BtoF 3'UTR _CAT- ₆₅₇ , showing _{biotransformation} from ricinoleic acid to an ester. Experiments were performed three times and error bars were expressed as standard deviations. The symbols are respectively ricinoleic acid (?), 12-ketooleic acid (?), Ester (11) and 11-hydroxyundec-9-enoic acid ).

7 is a graph showing the relationship between the length of the CAT sequence (3'UTR _CAT ) inserted in the 3'UTR and the initial bioconversion activity with the number of RNase E cleavage sites.
7A is a diagram showing the relationship between the length of the CAT sequence inserted in the 3'UTR and the initial organism conversion rate. Was calculated on the basis of the concentration of the product measured every 30 minutes (t = 0.5h) by GC / MS. The zero of the X-axis contains five RNase E cleavage sites and is a pNAP-binding site that expresses pCOLADuet-ADH and pET22b-BmoF1-3'UTR _native Indicates the initial bioconversion rate of recombinant E. coli BL21 (DE3).
FIG. 7B is a graph showing the relationship between the number of RNase E cleavage sites in the CAT sequence inserted in the 3'UTR and the initial bioconversion activity. 5 on the X-axis shows the initial bioconversion activity of recombinant E. coli BL21 (DE3) containing five RNase E cleavage sites and expressing pCOLADuet-ADH and pET22b-BmoF1-3'UTR _native . The red star shape contains 19 potential RNase E cleavage sites and represents the initial bioconversion activity of recombinant E. coli BL21 (DE3) expressing pCOLADuet-ADH and pET22b-BmoF1-3'UTR _hilD .
Figure 8 is a schematic diagram of sequence homology of MO16 and BmoF1 and SDS-PAGE analysis of recombinant E. coli BL21 (DE3) expressing pCOLADuet-ADH and pET22b-MO16.
Figure 8A is a schematic diagram showing that the sequence homology of MO16 and BmoF1 is 23.3%. FIG. 8B shows SDS-PAGE analysis of recombinant E. coli BL21 (DE3) expressing pCOLADuet-ADH and pET22b-MO16. Lane M is a marker, Lane S is a soluble fraction of recombinant E. coli BL21 (DE3) expressing pCOLADuet-ADH and pET22b-MO16, and Lane I is a soluble fraction of recombinant E. coli BL21 (DE3) expressing pCOLADuet-ADH and pET22b- Water-soluble fraction.
FIG. 9 is a graph showing the effect of the recombinant Escherichia coli BL21 (DE3) (lane WS and WI), recombinant Escherichia coli BL21 (DE3) pET22b-MO16-3'UTR _native (lane 1 and 2) PAGE analysis of cell extracts isolated from the culture medium of _CAT-257 (lane 3 and 4), and recombinant E. coli BL21 (DE3) pET22b-MO16-3'UTR _CAT- ₆₅₇ (lanes 5 and 6) . Lanes WS, 1, 3, and 5 represent water-soluble fractions of cell extracts. Lanes WI, 2, 4, and 6 represent non-aqueous fractions of cell extracts. Lane M represents a marker.
10 is a diagram showing the biological conversion process of ricinoleic acid. Ricinoleate converted to resan 1 is ω- hydroxy-undec-9-Ino acid (ω-hydroxyundec-9-enoic acid, 5) and n- heptanoic acid (n -heptanoic acid, 4) through a multi-step enzyme reaction do.
11 is a diagram showing the result of biotransformation from ricinoleic acid to an ester for recombinant E. coli BL21 (DE3) pET22b-MO16-3'UTR _CAT _.
A in Fig. 11 is a recombinant E. coli _{BL21 (DE3) pET22b-MO16-3'UTR native} , 11 B of the recombinant E. coli _{BL21 (DE3) pET22b-MO16-3'UTR CAT} -257, Figure 11 C is a recombinant E. coli BL21 (DE3) pET22b-MO16-3 ' UTR _CAT -657, showing _{biotransformation} from ricinoleic acid to an ester.
12 is a diagram showing the biological conversion process of linoleic acid. Linoleic acid (6) is converted to the number of steps through enzymatic reaction ω- hydroxy-9-deck also Ino acid (ω-hydroxydodec-9-enoic acid, 11) and the hexanoic acid n- (n -hexanoic acid, 10) .
13 is a diagram showing biotransformation from 13-hydroxyoleic acid to an ester to recombinant Escherichia coli BL21 (DE3) pET22b-MO16-3'UTR _CAT .
C of FIG. 13 A is a recombinant E. coli BL21 (DE3) pET22b-MO16-3'UTR _native, B is a recombinant E. coli BL21 (DE3) in Fig. 13 pET22b-MO16-3'UTR _CAT _-257, Figure 13 is a recombinant E. coli BL21 (DE3) is a diagram showing the result of a biotransformation using an ester in the pET22b-MO16-3'UTR _CAT _-657, 13- hydroxy oleic acid. Experiments were performed three times and error bars were expressed as standard deviations. Symbols are 13-hydroxyoleic acid (?), 13-ketooleic acid,?, Ester, and 11-hydroxydodec-9-enoic acid, ▲).

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and the scope of the present invention is not construed as being limited by these embodiments.

Example One. hilD In E. coli using the nucleotide sequence, Of BmoF1 Aqueous expression

BmoF1, a BVMO (Bayer-Villiger monooxygenase) of Pseudomonas fluorescens DSM 50106, known to catalyze an oxidation reaction by inserting a position-specific oxygen into the carbon skeleton adjacent to a keto group of various compounds desired, is expressed in a soluble form in E. coli strain (Fig. 1).

According to a recent study, the stability of Salmonella enterica hilD mRNA in E. coli was significantly affected by the presence of a 3 'untranslated reong consisting of 310 nucleotides containing 14 RNase E cleavage sites (Fig. 2A). For example, removal of the 3'UTR increases hilD mRNA levels, thereby enhancing gene expression in E. coli.

In the present invention, the 3'UTR engineering as described above can affect the local concentration of the BmoF1 polypeptide newly synthesized in the cytoplasm by the change in BmoF1 mRNA stability, thereby increasing the expression of the BmoF1 water soluble protein To investigate, the following experiment was carried out.

Example 1.1: By 3'UTR hilD Insertion of base sequences ( hilD 3'UTR , 3'UTR _hilD )on Following BmoF1 mRNA level analysis

Example 1.1.1: hilD Nucleotide sequence gene Cloning

In order to insert the hilD nucleotide sequence into the 3 'UTR of BmoF1 gene, Salmonella enterica hilD mRNA was synthesized in COSMOJINTECH and PCR was carried out using hilD_F (5'-AAGCTTCATTTTTTGTATCTGTCACTTAAG-3 ', SEQ ID NO: 1) and hilD_R (5'-CTCGAGAATAAAATGCCGGCCTTAATCC-3', SEQ ID NO: 2) as a forward primer and reverse primer And amplified through PCR (reaction) to separate the hilD nucleotide sequence (SEQ ID NO: 17). The amplified hilD sequence fragment corresponds to the 3'UTR of the BmoF1 gene and inserted into the HindIII-XhoI restriction enzyme site of pET22b- _BmoF1 to prepare E. coli BL21 (DE3) pET22b-BmoF1-3'UTR _hilD .

Example 1.1.2: RNA extraction and Reverse transcription PCR reverse transcription PCR Analysis of mRNA levels according to

In order to confirm the BmoF1-3'UTR _hilD mRNA level of the E. coli BL21 (DE3) pET-BmoF1-3'UTR _hilD prepared above, RNA extraction and reverse transcription PCR were performed using the E. coli-grown cells to obtain E. coli BL21 (DE3 ) pET-BmoF1-3'UTR the BmoF1-3'UTR _hilD _hilD mRNA levels were compared with E. coli BL21 (DE3) pET-BmoF1-3'UTR _native BmoF1-3'UTR _native mRNA levels.

First, the 1 mL aliquot obtained from the incubated cells on the stationary phase was centrifuged at 13,000 rpm and 4 ^° C for 5 minutes. The centrifuged extract was extracted with the RNeasy extraction kit (Qiagen) according to the manufacturer's instructions . The extracted total RNA was suspended in 50 μl of RNase-free water, and the RNA concentration of the suspended solution was confirmed by ND-1000 spectrophotometer.

The reverse transcriptase-polymerase chain reaction (RT-PCR) was performed using a HiPi ™ One-step 5X RT-PCR primer according to the manufacturer's instructions, and 10 nmol RNA template and 5 pmol primers were RT- PCR premix tube and RNase-free distilled water was added until it was up to 20 [mu] l.

The RT-PCR reaction was performed as follows.

The cDNA synthesis was carried out for 30 minutes at 42 DEG C for 1 cycle and at 94 DEG C for 5 minutes. Amplification was carried out at 94 ° C for 30 seconds, 60 ° C for 30 seconds and 72 ° C for 45 seconds. The primer sequence for amplifying the portion corresponding to BmoF1 was 5'-ATGAATGCCCACAGTGATTCCATCGACATCGCCATCATCGGTTCGGGTTTTGGTTCGGGTTTTGCCG-3 '(forward primer, SEQ ID NO: 3) and 5'-TGAGAGGCTGCCTTCTGCCGTGGAGTGGGGCGCCGTGGCCGGACGGGGCGGCGCA-3' (reverse primer, SEQ ID NO: 4). The PCR primer sequence for ihfB amplification as a control gene was 5'-ATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCG-3 '(forward primer, SEQ ID NO: 5) and 5'-TTAACCGTAAATATTGGCGCGATCGCGCAGTTCTTTACCAGGTTTAAAGTGAGGA-3' (reverse primer, SEQ ID NO: 6). RT-PCR was performed with the above primers PCR products were analyzed by 1% agarose gel electrophoresis.

As a result, the recombinant E. coli BL21 (DE3) pET-BmoF1-3'UTR _hilD BmoF1-3'UTR _hilD mRNA level of recombinant E. coli BL21 (DE3) pET-BmoF1-3'UTR _native BmoF1-3'UTR _native mRNA levels of (FIG. 3, A).

Thus, it was found that the hilD nucleotide sequence containing the RNase E cleavage site inserted into the 3 'UTR in E. coli influences mRNA stability.

Example 1.2: By 3'UTR hilD Insertion of base sequences ( hilD 3'UTR , 3'UTR _hilD Analysis of protein expression level

In order to confirm the BmoF1-3'UTR _hilD protein expression level of the recombinant E. coli BL21 (DE3) pET-BmoF1-3'UTR _hilD prepared _above , SDS-PAGE analysis was performed to obtain recombinant Escherichia coli BL21 (DE3) pET-BmoF1-3 "BmoF1-3'UTR _hilD the protein level of expression of UTR _hilD E. coli BL21 (DE3) pET-BmoF1 - were compared with the _native 3'UTR protein expression level - BmoF1 the _native 3'UTR.

First, to produce a protein sample, the cells were sonicated 6 times for 15 seconds with an amplitude of 25% and a 5 second hold for each ultrasonic treatment. The soluble protein lysate was separated by centrifugation at 13000 rpm for 10 min at 4 ° C and 50 mM Tris-HCl buffer (pH 8.0) for the remaining non-water soluble protein was added to the same volume as the soluble protein lysate Was added. The water-soluble and non-water-soluble proteins were heat-denatured (at 5O < 0 > C for 5 min) and mixed with 5X sample buffer (Elpis Biotech, Korea). The water-soluble and non-water-soluble protein samples prepared by the above method were loaded on a 10% sodium dodecyl sulfate polyacrylamide gel (10% sodium dodecyl sulfate polyacrylamide gel) and then loaded onto Coomassie Brilliant Blue R-250 Lt; / RTI >

As a result, the recombinant Escherichia coli BL21 (DE3) pET-BmoF1-3'UTR _hilD BmoF1-3'UTR _hilD Total protein expression levels of recombinant E. coli BL21 (DE3) pET-BmoF1 - BmoF1 the _native 3'UTR - lower than the _native 3'UTR total protein expression levels, while the BmoF1-3'UTR _hilD The water-soluble protein expression level was found to be significantly higher than the water - soluble protein expression level of BmoF1-3'UTR _native (Fig. 3B).

In this way, the hilD nucleotide sequence containing the RNase E cleavage site was inserted into the 3'UTR to reduce the BmoF1 mRNA stability, thereby improving the level of soluble protein expression of BmoF1.

Example 1.3: By 3'UTR hilD Insertion of base sequences ( hilD 3'UTR , 3'UTR _hilD Comparison of catalytic activity of BmoF1 according to

Example 1.3.1: Whole cell Biotransformation

Since BmoF1 is known to be too unstable to separate out of the extracellular form, the catalytic activity of BmoF1 is to be verified by whole cell biotransformation. Micrococcus , which not only expresses BmoF1 but also produces a substrate for BmoF1 long-chain secondary alcohol-decomposing enzyme luteus NCTC2665 recombinant E. coli expressing the (long chain secondary alcohol dehydrogenase) ( E. Coli) BL21 (DE3) to prepare a pCOLA-ADH and pET22b-BmoF1-3'UTR _hilD a whole cell biotransformation Respectively.

First, the recombinant cells were cultured in a regenberg medium at 30 ° C, and expression of the target gene was induced with 0.1 mM IPTG at 0.6 OD600. Thereafter, the incubation temperature was reduced to 20 占 폚. When the culture reaches the stopper, the alcohol dehydrogenase (ADH) derived from Micrococcus luteus NCTC 2666, M. luteus NCTC 2666 and the Pseudomonas fluorescens DSM 50106 ( P. fluorescens DSM 50106) derived from Pseudomonas fluorescens DSM 50106 Recombinant E. coli BL21 (DE3) pCOLADuet-ADH expressing BVMO and pET22b-BmoF1 were obtained by centrifugation at 3500 g for 20 min at 4 ° C and 7.2 g dried cells (pH 8.0) in 50 mM Tris-HC buffer dry cell) / L was suspended. Bioconversion was initiated by the addition of 5 mM ricinoleic acid and 0.5 g / L Tween 80 to 50 mM Tris-HCl buffer (pH 8.0) and run in shaking incubator at 35 ° C and 200 rpm.

Example 1.3.2: GC / MS analysis

GC / MS analysis was carried out in order to confirm the change of the reactants upon conversion of whole cell biotransformation after the whole cell biotransformation was performed in the same manner as in Example 1.3.1.

The reaction medium was mixed with an amount corresponding to three times that of ethyl acetate containing 5 g / L palmitic acid as an internal standard. The organic phase was obtained after vigorous vortexing and then derivatized with N-methyl-N- (trimethylsilyl) trifluoroacetamide (TMS). The TMS derivative was analyzed using a Thermo Ultra Trace GC system connected to an ion trap mass detector. The derivatives were separated on a non-polar capillary column (30-m length, 0.25-μm film thickness, HP-5MS, Aligent). Linear temperature changes were programmed as follows (90 ° C, 5 ° C / min to 280 ° C). The injection port temperature was 230 ° C.

The mass spectra were obtained by electron impact ionization at 70 eV. The scan spectrum was obtained within the range of 100 to 600 m / z. Selected ion monitoring (SIM) was used for detection and fragmentation of reaction products. The concentrations of the reaction substrates and products were determined based on calibration curves determined using the products isolated in the laboratory or commercially available products.

Example 1.3.3: Comparative analysis of catalytic activity through whole cell bioconversion

The whole cell biotransformation was carried out by the methods described in Examples 1.3.1 and 1.3.2 above and the obtained product was measured by GC / MS to obtain recombinant Escherichia coli BL21 (pCOLA-ADH) expressing pCOLA-ADH and pET22b-BmoF1-3'UTR _hilD DE3) were compared with recombinant Escherichia coli BL21 (DE3) expressing pCOLA-ADH and pET22b-BmoF1-3'UTR _native .

Adding ricinoleate in the culture medium of recombinant E. coli BL21 (DE3) of the stopper expressing pCOLA-ADH and pET22b-BmoF1-3'UTR _hilD, expressing pCOLA-ADH and pET22b-BmoF1-3'UTR _hilD In the recombinant E. coli BL21 (DE3), the ester product by BmoF1 was significantly higher than the recombinant E. coli BL21 (DE3) expressing pCOLA-ADH and pET22b-BmoF1-3'UTR _native (FIG.

The above results show that insertion of the hilD nucleotide sequence containing the RNase E cleavage site at the 3'UTR reduces the stability of BmoF1 mRNA, which in turn decreases the level of BmoF1 total protein expression, but BmoF1 (SEQ ID NO: It was found that the expression of water - soluble protein was increased and the water - soluble expression and bioconversion activity of BmoF1 enzyme were also influenced.

Example 2. Protein using CAT nucleotide sequence Of BmoF1 Aqueous expression

As a result of inserting the hilD nucleotide sequence containing the RNase E cleavage site into the 3'UTR through the Example 1, it was confirmed that the stability of BmoF1 mRNA was decreased and the expression of the soluble protein of BmoF1 was increased in E. coli. To further investigate the effect of 3'UTR engineering, E. coli chloramphenicol acetyltransferase was coded and a CAT nucleotide sequence (Figure 2B) containing an RNase E cleavage site estimated at 28 The following experiment was carried out.

Example 2.1: By 3'UTR Insertion of CAT nucleotide sequence ( 3'UTR _CAT )In accordance mRNA Level and Protein Expression Level Analysis

Example 2.1.1: gene of CAT nucleotide sequence Cloning

The CAT nucleotide sequence was amplified from pACYCDuet-1 vector by PCR using CAT657_F (5'-AAGCTTATGGAGAAAAAAATCACTGGATAT-3 ', SEQ ID NO: 7) and CAT657_R (5'-CTCGAGGGCATCAGCACCTTGTCG-3', SEQ ID NO: 8) primers as forward primer and reverse primer Respectively. The amplified CAT nucleotide sequence is a CAT657 nucleotide sequence (SEQ ID NO: 21) containing 657 nucleotides and is inserted into the HindIII-XhoI restriction enzyme site of pET22b-BmoF1 in the 3'UTR of the BmoF1 gene to obtain recombinant Escherichia coli BL21 (DE3) pET22b-BmoF1-3'UTR _CAT was prepared.

Example 2.2.2: mRNA Level and Protein Expression Level Analysis

In order to confirm the BmoF1-3'UTR _CAT657 mRNA level of the recombinant E. coli BL21 (DE3) pET22b-BmoF1-3'UTR _CAT657 prepared above, RNA extraction and reverse transcription PCR were carried out using the cells cultured with the recombinant E. coli, the BL21 (DE3) pET-BmoF1-3'UTR BmoF1-3'UTR _CAT657 mRNA levels of _CAT657 were compared with BmoF1-3'UTR _native mRNA level of recombinant E. coli _{BL21 (DE3) pET-BmoF1-3'UTR native} .

RNA extraction and reverse transcription for mRNA level analysis was performed in the same manner as described in Example 1.1.2.

As a result, the recombinant E. coli BL21 (DE3) pET-BmoF1-3'UTR BmoF1-3'UTR _CAT657 mRNA levels of _CAT657 is recombinant E. coli BL21 (DE3) pET-BmoF1-3'UTR _native BmoF1-3'UTR _native mRNA levels of (A in FIG. 5).

This confirmed that the CAT nucleotide sequence containing the RNase E cleavage site affected mRNA stability and showed that the sequence could be used as an activated 3'UTR.

Next, the thus-prepared recombinant Escherichia coli BL21 (DE3) pET-BmoF1-3'UTR _CAT657 BmoF1-3'UTR _CAT657 In order to confirm the protein expression level, SDS-PAGE analysis and Western blot were performed to _determine the BmoF1-3'UTR _CAT657 (SEQ ID _NO : 1) of recombinant Escherichia coli BL21 (DE3) pET-BmoF1-3'UTR _CAT657 The protein expression levels of recombinant E. coli BL21 (DE3) pET-BmoF1 - were compared with the _native 3'UTR protein expression level - BmoF1 the _native 3'UTR.

SDS-PAGE analysis was performed in the same manner as described in Example 1.2, and western blotting was performed in the following manner.

The membranes were blocked overnight in PBS containing 5% skim milk and 0.5% Tween 20, and the anti-His primary antibody (IG Therapy Co., Ltd. Tokyo, Japan) diluted 1: 5000 in PBST-5% And incubated together at room temperature for 1 hour. After washing with PBS containing 0.5% Tween 20, the membranes were incubated with goat anti-mouse IgG (Abcam, Cambridge, UK) conjugated with alkaline phosphatase diluted 1: 500 with PBST-5% And incubated together at room temperature for 1 hour. The blot was developed using a BCIP / NBT solution substrate (Abcam).

After performing SDS-PAGE and Western blot, BmoF1-3'UTR _CAT657 total protein level of expression of the recombinant E. coli BL21 (DE3) pET-BmoF1-3'UTR _CAT657 is recombinant E. coli BL21 (DE3) pET-BmoF1 - 3'UTR total _native 3'UTR natatjiman than protein expression level (C in Fig. 5) water-soluble protein level of expression of BmoF1-3'UTR _CAT657 is BmoF1 - - BmoF1 of _native soluble protein level of expression of _native 3'UTR was significantly increased (Fig. 5 B).

This suggests that the insertion of the CAT nucleotide sequence containing the RNase E cleavage site reduced the stability of BmoF1 mRNA and increased the expression of soluble proteins of BmoF1.

Example 2.2: By 3'UTR Inserted RNase E Depending on the number of cutting sites mRNA Level and Protein Expression Level Analysis

Example 2.2.1: gene Cloning

To determine if the number of RNase E cleavage sites present in the CAT nucleotide sequence is associated with mRNA stability, a set of variants including partial deletion of the CAT nucleotide sequence (3'UTR _CAT ) inserted in the 3'UTR was constructed.

The partially deleted fragments of the CAT nucleotide sequence inserted in the 3'UTR were amplified by PCR using the forward primer CAT_F and the other reverse primer CAT257_R (5'-CTCGAGCGCCCCGCCCTGCCACTCATCG-3 ', SEQ ID NO: 9), CAT357_R (5'-CTCGAGTCCCATATCACCAGCTCA- CAT357 (SEQ ID NO: 19) and CAT557 (SEQ ID NO: 20) were amplified by PCR using CAT557_R (5'-CTCGAGTATGTGTAGAAACTGCCG-3 ', SEQ ID NO: -BmoF1. &Lt; / RTI >

As a result, mutants containing 257, 357, and 557 nucleotides from the 5 'region of the CAT nucleotide sequence (partially deleted fragments of the CAT nucleotide sequence inserted into the 3' UTR) increased in length as the length of the CAT sequence increased The number of RNase E cleavage sites was increased stepwise (as shown in FIG. 2B and FIG. 4).

Example 2.2.2: mRNA Level and Protein Expression Level Analysis

In order to confirm the change of mRNA stability according to the number of RNase E cleavage sites present in the CAT nucleotide sequence, RNA extraction and reverse transcription PCR were carried out to obtain recombinant Escherichia coli BL21 (DE3) pET22b-BmoF1 having different numbers of RNase E cleavage sites -3'UTR _CAT The BmoF1-3'UTR _CAT mRNA levels of mutants were compared and analyzed.

As a result, as the length of the CAT nucleotide sequence increased from 257 nucleotides to 657 nucleotides, the recombinant E. coli BL21 (DE3) pET22b-BmoF1-3'UTR _CAT The BmoF1-3'UTR _CAT mRNA levels of mutants gradually decreased (Fig. 5A)

These results indicate that the mRNA stability decreases as the number of RNase E cleavage sites in the CAT nucleotide sequence increases.

Next, in order to confirm the change of the protein expression level according to the number of RNase E cleavage sites existing in the CAT nucleotide sequence, SDS-PAGE analysis was carried out to obtain recombinant Escherichia coli BL21 (DE3 ) pET22b-BmoF1-3'UTR _CAT variants were compared and analyzed for their BmoF1-3'UTR _CAT protein expression levels.

As a result, as the number of RNase E cleavage sites in the CAT nucleotide sequence increased, recombinant E. coli BL21 (DE3) pET22b-BmoF1-3'UTR _CAT The BmoF1-3'UTR _CAT total protein expression level of the mutants was decreased (FIG. 5C), but the recombinant E. coli BL21 (DE3) pET22b-BmoF1-3'UTR _CAT The level of BmoF1-3'UTR _CAT- soluble protein expression of mutants increased (FIG. 5B).

As a result, the number of RNase E cleavage sites in the CAT nucleotide sequence increased and the expression of soluble protein increased.

Example 2.3: RNase E Depending on the number of cutting sites Biocatalyst Comparative analysis of changes in activity

PCOLA-ADH and pET22b-BmoF1-UTR _native , or pCOLA-ADH and pET22b-BmoF1-UTR _CATs , respectively, in order to investigate the effect of the number of RNase E cleavage sites inserted in the 3'UTR on the biocatalytic activity of BmoF1 in Escherichia coli The recombinant E. coli BL21 (DE3) variants expressing the mutants were subjected to whole cell biotransformation by reaction with ricinoleic acid.

The reaction conditions were the same as those shown in Fig. 3C, and whole cell biotransformation and measurement of reaction products were carried out in the same manner as in Examples 1.3.1 and 1.3.2.

As a result, recombinant Escherichia coli BL21 expressing pCOLA-ADH and pET22b-BmoF1-3'UTR _CAT257 /, pET22b-BmoF1-3'UTR _CAT357 / pET22b-BmoF1-3'UTR _CAT557 / pET22b-BmoF1-3'UTR _CAT657 mutants (DE3) showed more bioconversion activity than that of recombinant E. coli BL21 (DE3) expressing pCOLA-ADH and pET22b-BmoF1-3'UTR _native (Fig. 6 and Table 1).

Relative mRNA levels ^[a] Initial bioconversion activity
(U g CDW ^-1 ) ^[b] Product yield (%)
(%) ^[c] bmoF1 -3'UTR _native 1.0 2.26 31.2 bmoF1 -3'UTR _CAT _-257 0.86 3.59 61.4 BmOF1 -3'UTR _CAT _-357 0.79 4.23 76.8 bmoF1 -3'UTR _CAT _-557 0.54 5.05 78.0 bmoF1 -3'UTR _CAT _-657 0.50 5.32 82.6

[a] Relative mRNA levels were measured from the electrophoresis results as shown in FIG. 5 by a densitometer.

[b] The initial bioconversion activity was calculated based on the product concentration, which was measured every 30 minutes by GC / MS, and all experiments were performed three times.

[c] production rate was measured based on substrate consumption and product concentration, which was measured by GC / MS and all experiments were performed three times.

In addition, the ester concentration was measured every 30 minutes to confirm that the ester product was increased, and it was confirmed that there was a very high correlation between the initial bioconversion activity and the number of RNase E cleavage sites (FIG. 7). Therefore, it was confirmed that the initial bioconversion activity increased as the number of RNase E cleavage sites increased.

Also in Example 1.3, the initial bioconversion activity of recombinant E. coli BL21 (DE3) expressing pCOLA-ADH and pET22b-BmoF1-3'UTR _hilD was _shown to be related to the number of RNase E cleavage sites. , It can be concluded that the number of RNase E cleavage sites is more important for bioconversion activity than the 3'UTR sequence and that the level of BmoF1 soluble protein expression also depends on the number of RNase E cleavage sites in the 3'UTR.

Example 3. Using the CAT nucleotide sequence Rhodococcus jostii RHA1 origin Of BVMO Aqueous expression

Pseudomonas fluorescein sense (Pseudomonas 　 fluorescens Similar to BmoF1 as BVMO (Bayer-Villiger monooxygenase) of DSM50106, Rhodococcus jostii MO16, which is RHA1-derived BVMO (i.e., MO16, sequence homology with BmoF1: 23.3%, Fig. 8A), is very difficult to express a soluble form in E. coli cells (Fig. Therefore, SDS-PAGE analysis and whole-cell biotransformation were performed to examine the effect of MOE 16 on the expression of water-soluble proteins of E. coli according to the number of RNase E cleavage sites present in the CAT sequence at 3'UTR.

Example 3.1: gene Cloning

To make pET22b-MO16, The MO16 gene of Rhodococcus jostii was subjected to PCR using MO16_F (5'-CATATGTCACACACCGAGACCGCCGCCGA-3 ', SEQ ID NO: 12) and MO16_R (5'-GGATCCCGGGCGGCTGAAGGTCATGGCGTCGTCC-3', SEQ ID NO: 13) as forward and reverse primers And amplified from the pET-MO16 vector. The MO16_CAT nucleotide sequence is represented by SEQ ID NO: 16), MO16_CAT657_F (5'-AAGCTTATGGAGAAAAAAAATCACTGGATATA-3 ', SEQ ID NO: Lt; / RTI > vector. The MO16_CAT sequence fragment was subcloned into the HindIII-XhoI restriction enzyme site of pET22b- _MO16 to construct pET22b-MO16-3'UTR _CAT657 and pET22b-MO16-3'UTR _CAT257 , respectively. All prepared plasmid sequences were confirmed by Macrogen (Seoul, Korea).

Example 3.2: Analysis of protein expression level

(DE3) pET22b-MO16-3'UTR _CAT257 and recombinant Escherichia coli BL21 (DE3) pET22b (DE3) were performed by SDS-PAGE analysis to confirm protein expression levels according to the number of RNase E cleavage sites present in the CAT sequence -MO16-3'UTR _CAT657 was compared to the level of MO16-3'UTR _native protein expression of recombinant E. coli BL21 (DE3) pET22b-MO16-3'UTR _native .

As a result, MO16-3'UTR _CAT657 showed no expression in E. coli and showed almost no expression of MO16-3'UTR _CAT657 protein of recombinant E. coli BL21 (DE3) pET22b-MO16-3'UTR _{CAT657 on} SDS-PAGE analysis (Fig. 9). In contrast, recombinant E. coli BL21 (DE3) pET22b-MO16-3'UTR _CAT257 MO16-3'UTR _CAT257 protein expression recombinant E. coli BL21 (DE3) pET22b-MO16-3'UTR _native MO16-3'UTR _native protein expressed in the (Fig. 9). As shown in Fig.

It was also confirmed that the expression of water-soluble protein can be improved by using the number of RNase E cleavage sites present in the CAT nucleotide sequence in the MO16 enzyme. Further, the 3'UTR can be used to detect the RNase E cleavage site in the CAT nucleotide sequence The water soluble protein expression of MO16 was more sensitive than the BVMO soluble protein expression.

Example 3.3: Whole cell biotransformation

In order to investigate the effect of the number of RNase E cleavage sites inserted in the 3'UTR in the biocatalytic activity of BmoF1 in Escherichia coli, recombinant Escherichia coli BL21 (DE3) expressing pET22b-MO16-3'UTR _native , pET22b-MO16- Recombinant E. coli BL21 (DE3) expressing 3'UTR _CAT257 and recombinant Escherichia coli BL21 (DE3) expressing pET22b-MO16-3'UTR _CAT657 were reacted with ricinoleic acid to effect bioconversion.

Whole cell biotransformation and reaction product measurements were swim in the same manner as in Examples 1.3.1 and 1.3.2.

As a result, recombinant Escherichia coli BL21 (DE3) expressing pET22b-MO16-3'UTR _CAT657 had no ester formation in the reaction product with respect to MO16 in the culture medium (compound corresponding to 3 in Fig. 10), 12 -Ketooleic acid (compound corresponding to 2 in FIG. 10) accumulated in the culture medium, MO16 was hardly expressed in E. coli (FIG. 11).

However, recombinant E. coli BL21 (DE3) expressing pET22b-MO16-3'UTR _CAT257 formed an ester product at a 35% higher rate than recombinant E. coli BL21 (DE3) expressing pET22b-MO16-3'UTR _native To improve the catalytic activity of MO16 (FIG. 11).

This tendency was also observed in the conversion of 13-hydroxy oleic acid to whole cell biology (FIGS. 12 and 13).

Therefore, through the above results, the MO16 water-soluble protein was increased through the insertion of the RNase E cleavage site and the number of RNase E cleavage sites present in the CAT nucleotide sequence, thereby improving the water-soluble expression and biotransformation activity of the MO16 enzyme in the microorganism Could know.

<110> Ewha University - Industry Collaboration Foundation <120> 3'UTR engineering to improve soluble expression of foreign proteins in microbial cells <130> KPA151267-KR <160> 21 <170> Kopatentin 2.0 <210> 1 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> hilD_F primer <400> 1 aagcttcatt ttttgtatct gtcacttaag 30 <210> 2 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> hilD_R primer <400> 2 ctcgagaata aaatgccggc cttaatcc 28 <210> 3 <211> 55 <212> DNA <213> Artificial Sequence <220> <223> BmoF1 forward primer <400> 3 atgaatgccc acagtgattc catcgacatc gccatcatcg gttcgggttt tgccg 55 <210> 4 <211> 55 <212> DNA <213> Artificial Sequence <220> <223> BmoF1 reverse primer <400> 4 tgagaggctg ccttctgccg tggagtgggg cgccgtggcc ggacggggcg gcgca 55 <210> 5 <211> 55 <212> DNA <213> Artificial Sequence <220> <223> IhFB forward primer <400> 5 atgaccaagt cagaattgat agaaagactt gccacccagc aatcgcacat tcccg 55 <210> 6 <211> 55 <212> DNA <213> Artificial Sequence <220> <223> ihfB reverse primer <400> 6 ttaaccgtaa atattggcgc gatcgcgcag ttctttacca ggtttaaagt gagga 55 <210> 7 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> CAT657_F primer <400> 7 aagcttatgg agaaaaaaat cactggatat 30 <210> 8 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> CAT657_R primer <400> 8 ctcgagggca tcagcacctt gtcg 24 <210> 9 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> CAT257_R primer <400> 9 ctcgagcgcc ccgccctgcc actcatcg 28 <210> 10 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> CAT357_R primer <400> 10 ctcgagtccc atatcaccag ctca 24 <210> 11 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> CAT557_R primer <400> 11 ctcgagtatg tgtagaaact gccg 24 <210> 12 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> MO16 forward primer <400> 12 catatgtcac acaccgagac cgccgccga 29 <210> 13 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> MO16 reverse primer <400> 13 ggatcccggg cggctgaagg tcatggcgtc gtcc 34 <210> 14 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> MO16_CAT657_F primer <400> 14 aagcttatgg agaaaaaaat cactggatat 30 <210> 15 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> MO16_CAT657_R primer <400> 15 ctcgagcgcc ccgccctgcc actcatcg 28 <210> 16 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> MO16_CAT257_R primer <400> 16 ctcgagcgcc ccctgccact catcg 25 <210> 17 <211> 310 <212> DNA <213> Artificial Sequence <220> <223> recombinant hilD sequence <400> 17 cattttttgt atctgtcact taagtaaaga tttttattaa aattgtaata atttaaaatt 60 cagactgcgc attaacacgc tctatcagga tgggaggcta ttcaatatca ttgttctgtc 120 cggaagacag cttatactga tatctatggt aatttaaagt aaggctgatt atataacacg 180 atttttgtga acttgtcatc gctatgatga ctggtaaaac gatattgcct tattcacagc 240 gtaagaattc gtccagatga cactatctcc ttccggcttt aaccctgtgg attaaggccg 300 gcattttatt 310 <210> 18 <211> 257 <212> DNA <213> Artificial Sequence <220> <223> recombinant CAT257 sequence <400> 18 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 60 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat 120 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt 180 cacattcttg cccgcctgat gaatgctcat ccggagttcc gtatggcaat gaaagacggt 240 gagctggtga tatggga 257 <210> 19 <211> 357 <212> DNA <213> Artificial Sequence <220> <223> recombinant CAT357 sequence <400> 19 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 60 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat 120 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt 180 cacattcttg cccgcctgat gaatgctcat ccggagttcc gtatggcaat gaaagacggt 240 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa 300 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacata 357 <210> 20 <211> 557 <212> DNA <213> Artificial Sequence <220> <223> recombinant CAT557 sequence <400> 20 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 60 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat 120 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt 180 cacattcttg cccgcctgat gaatgctcat ccggagttcc gtatggcaat gaaagacggt 240 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa 300 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat 360 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag 420 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg 480 gccaatatgg acaacttctt cgcccccgtt ttcactatgg gcaaatatta tacgcaaggc 540 gacaaggtgc tgatgcc 557 <210> 21 <211> 657 <212> DNA <213> Artificial Sequence <220> <223> recombinant CAT657 sequence <400> 21 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 60 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat 120 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt 180 cacattcttg cccgcctgat gaatgctcat ccggagttcc gtatggcaat gaaagacggt 240 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa 300 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat 360 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag 420 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg 480 gccaatatgg acaacttctt cgcccccgtt ttcactatgg gcaaatatta tacgcaaggc 540 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat 600 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcg 657

Claims

(a) obtaining a polynucleotide bound to an untranslated region (UTR) that increases mRNA stability at the 3 'end of a gene encoding a desired water-soluble protein; And
(b) expressing the polynucleotide,
Wherein a base sequence including an RNase E cleavage site which lowers the stability of mRNA is inserted into the nucleotide sequence of the UTR to increase the water-soluble expression ratio relative to the total expression level of the desired protein.

[Claim 2] The protein according to claim 1, wherein when the desired water-soluble protein is over-expressed, the over-expressed protein co-aggregates in the cell to form an inclusion body, whereby the water-soluble expression ratio of the desired protein is 50% In method.

The method according to claim 1, wherein the desired water-soluble protein is Pseudomonas fluorescens DSM 50106 or Rhodococcus jostii RHA1-derived BVMO (Bayer-Villiger monooxygenase).

4. The method of claim 3 wherein the Pseudomonas fluorescein sense (Pseudomonas 　 fluorescens DSM50106 derived BVMO is BmoF1.

4. The method according to claim 3, wherein the Rhodococcus jostii ) Wherein the RHA1 derived BVMO is MO16.

2. The method according to claim 1, wherein the water-soluble expression ratio of the target protein with respect to the total expression level of the target protein is changed by adjusting the number of RNase E cleavage sites.

[Claim 7] The method according to claim 6, wherein when BmoF1 is expressed, the water-soluble expression ratio with respect to the total expression level of the desired protein is increased as the number of RNase E cleavage sites increases.

2. The method of claim 1, wherein the number of RNase E cleavage sites inserted into the UTR is between 10 and 100.

The method according to claim 1, wherein the base sequence comprising the RNase E cleavage site is all or part of a hilD base sequence, CAT base sequence.

10. The method of claim 9, wherein the number of RNase E cleavage sites included in the hilD base sequence is from 12 to 16.

10. The method according to claim 9, wherein the number of RNase E cleavage sites included in the CAT nucleotide sequence is 13-30.

A multicloning site into which the gene can be introduced so that the UTR (untranslated region) -binding polynucleotide that increases the mRNA stability at the 3 'end of the gene encoding the desired water soluble protein can be expressed, An increase in the water-soluble expression ratio relative to the total expression level of the target protein, including the UTR (untranslated region), which is inserted at the 3 'end of the multiple cloning site and inserted with a nucleotide sequence containing an RNase E cleavage site which lowers the stability of mRNA Expression vector.

13. A transformant, wherein the expression vector of claim 12 is introduced to increase an aqueous expression level relative to the total expression level of a desired protein.