KR20170057448A - Production method for noodles, and noodle separation improver - Google Patents
Production method for noodles, and noodle separation improver Download PDFInfo
- Publication number
- KR20170057448A KR20170057448A KR1020177011779A KR20177011779A KR20170057448A KR 20170057448 A KR20170057448 A KR 20170057448A KR 1020177011779 A KR1020177011779 A KR 1020177011779A KR 20177011779 A KR20177011779 A KR 20177011779A KR 20170057448 A KR20170057448 A KR 20170057448A
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- South Korea
- Prior art keywords
- lipase
- amylase
- water
- noodles
- raw material
- Prior art date
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/109—Types of pasta, e.g. macaroni or noodles
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
- A21D2/36—Vegetable material
- A21D2/362—Leguminous plants
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D8/00—Methods for preparing or baking dough
- A21D8/02—Methods for preparing dough; Treating dough prior to baking
- A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
- A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/50—Polysaccharides, gums
- A23V2250/51—Polysaccharide
- A23V2250/5108—Cellulose
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Noodles (AREA)
Abstract
[문제] 본 발명은, 보존에 있어 면의 분리성이 감소하는 등의 품질저하를 억제하여 효율적으로 맛있는 면류, 그 면류를 제조하는 방법 및 면류의 분리 개량제를 제공하는 것을 목적으로 한다. [해법] 리파아제 중에서도 1,3-위치특이성을 갖는 리파아제 및 아밀라아제를 면에 첨가하는 것으로, 보존 중인 경우의 면의 분리가 나빠지는 것을 극적으로 개량할 수 있고, 수용성 헤미셀룰로오스를 추가로 병용하여 면의 분리성을 더욱 개량할 수 있다. [Problem] It is an object of the present invention to provide a delicious noodle, a method of producing the noodle efficiently, and a separation improving agent of noodle by suppressing quality deterioration such as deterioration in surface separation in preservation. [Solution] By adding lipase and amylase having 1,3-position specificity among lipases to the cotton, it is possible to dramatically improve the deterioration of the separation of the cotton in the case of preservation, and further, water-soluble hemicellulose is additionally used, The separability can be further improved.
Description
본 발명은 면류의 제조방법 및 면용 분리(Separation) 개량제에 관한 것이다.The present invention relates to a method for producing noodles and a separating agent for cotton.
최근 식품의 다양화로 인해, 가공식품에 대한 니즈가 증가하고 있다. 특히 면류는, 대량으로 가공·조리된 제품이 편의점, 슈퍼마켓 등에서 판매되어 많은 소비자가 먹기 시작했다.Recently, due to diversification of foods, needs for processed foods are increasing. In particular, noodles are sold in convenience stores, supermarkets, etc., and many consumers have begun eating them.
이러한 유통·시판되는 가공 식품에 있어서, 특히 문제가 되는 것이 가공의 편리성 및 시간 경과에 따른 품질 열화(劣化)이다. 면류는 일반적으로 전분을 포함하고 있어, 가공·조리시, 식품 표면으로 전분질이 흘러 나와 식품끼리 서로 결착(結着)하게 된다. 그 결과, 면끼리의 부착으로 인해, 1회분으로 소분하거나 섭취 시에 국물이나 소스와 섞거나 하는 것이 곤란해진다. 즉석면의 경우, 면이 결착된 부분이 뜨거운 물로 회복되기 어려워진다. 이러한 문제는 생산효율의 저하와 비용 증가를 초래하고, 뿐만 아니라 완성된 음식도 먹기 힘들고, 맛이 없게 되는 결과를 초래한다.In such distributed and commercially available processed food products, particular problems are processing convenience and quality deterioration with time. Noodles generally contain starch, and during processing and cooking, the starch flows out to the food surface, causing the food to bind to each other. As a result, due to the adhesion of the surfaces, it becomes difficult to subculture in one batch or to mix with broth or sauce at the time of ingestion. In the case of the instant surface, the portion to which the surface is bonded becomes difficult to recover by hot water. These problems result in a decrease in production efficiency and an increase in cost, as well as a difficulty in eating the finished food and a lack of taste.
이러한 식품끼리의 결착성을 없애고, 분리성을 개선하는 종래의 방법으로는, 면 가닥이 분리되는 기능을 갖는 액체를 첨가하는 방법이 알려져 있다. 예를 들어, 유지 또는 유화유지(乳化油脂)를 혼합하는 방법이 있지만(특허문헌 1), 유지를 분리제로 사용하는 경우 섭취 시 국물에 기름 부유가 발생하고, 소면이나 소바, 우동과 같은 면류에도 사용할 수 없다. 그 외, HLB가 높은 자당 지방산 에스테르(sucrose fatty acid ester)를 첨가하는 방법 (특허문헌 2), 유기산을 첨가하는 방법 (특허문헌 3), 기계적인 진동을 부여하면서 가공하는 방법 (특허문헌 4), 수용성 헤미 셀룰로오스를 사용하는 방법 (특허문헌 4) 등이 있지만, 어떤 경우에도, 충분한 분리성을 얻지 못하고, 유화제를 단독 사용하면 식감이 나빠지는 문제점이 있다.As a conventional method for eliminating the binding property between these foods and improving the separability, a method of adding a liquid having a function of separating the cotton strands is known. For example, there is a method of mixing oil or fat retaining oil (emulsified oil) (Patent Document 1). However, when oil is used as a separating agent, oil stagnation occurs in the broth during ingestion, Can not use it. In addition, a method of adding a sucrose fatty acid ester having a high HLB (Patent Document 2), a method of adding an organic acid (Patent Document 3), a method of processing while imparting mechanical vibration (Patent Document 4) , And a method using water-soluble hemicellulose (Patent Document 4). In any case, however, sufficient separability can not be obtained, and when the emulsifier is used alone, the texture is deteriorated.
종래부터, 면 반죽에 효소를 첨가하는 방법이 알려져 있다. 예를 들면, 특허문헌 5-9에는 리파아제를 면 반죽에 첨가하는 방법이 기재되어 있다. 어느 것에 있어서도, 식감이나 분리를 개선하는 효과는 확인되었지만, 그 효과는 충분하지 않다. Conventionally, a method of adding an enzyme to a cotton dough is known. For example, Patent Document 5-9 describes a method of adding lipase to cotton dough. In either case, the effect of improving texture and separation was confirmed, but the effect is not sufficient.
아밀라아제의 첨가에 대해서도 특허문헌 10에 기재되어 있지만, 분리성의 효과가 충분하지 않을 뿐만 아니라, α화된 면 가닥을 아밀라아제로 처리하고, 가열에 의해 비활성화하는 방법이고, 간편성이 결여되는 문제가 있다. The addition of amylase is also described in Patent Document 10, but the effect of separability is not sufficient, and the method of treating the α-amylase with amylase and deactivating it by heating is problematic in that it lacks simplicity.
본 발명은, 보존 시 분리가 나빠지는 등의 품질 저하를 억제하고, 효율적으로 맛있는 면류, 그 면류의 제조방법, 면류의 분리 개량제를 제공하는 것을 목적으로 한다. It is an object of the present invention to provide a noodle, a method of producing the same, and a separation improving agent for the noodle efficiently, by suppressing deterioration in quality such as deterioration in separation during preservation.
본 발명자들은 상기 문제점들을 고려하여, 예의 연구를 거듭한 결과, 1,3-위치 특이성을 갖는 리파아제와 아밀라아제를 면에 첨가함으로써, 면의 탄력성이나 찰기와 같은 식감이나 풍미에 영향이 없이, 보존 중에 분리성이 나빠지는 것이 극적으로 개량되는 것을 발견하였다. 또한, 수용성 헤미 셀룰로오스를 병용하는 것으로, 면의 분리가 한층 더 개량되는 것을 발견함으로써 본 발명을 완성하였다.DISCLOSURE OF THE INVENTION The inventors of the present invention have conducted intensive studies in view of the above problems. As a result, it has been found that lipase and amylase having 1,3-position specificity can be added to a cotton face to improve the elasticity and stickiness, It was found that the deterioration of the separability was dramatically improved. Further, the present inventors have completed the present invention by discovering that the separation of the surface is further improved by using water-soluble hemicellulose in combination.
즉, 본 발명은,That is,
(1) 원료 분말 1g당 1 내지 20unit의 1,3-위치 특이성을 갖는 리파아제와 원료 분말 1g당 0.1 내지 5unit의 아밀라아제를 반죽에 첨가하는 것을 특징으로 하는, 면류의 제조방법,(1) a process for producing noodles, which comprises adding 1 to 20 units of lipase having 1, 3-position specificity per 1 g of raw material powder and 0.1 to 5 units of amylase per 1 g of raw material powder to the dough,
(2) 아밀라아제는 α-아밀라아제인, (1) 기재의 면류의 제조방법,(2) The amylase is an? -Amylase, a method for producing the noodles described in (1)
(3) 수용성 헤미 셀룰로오스를 더 포함하는, (1) 또는 (2)에 기재된 면류의 제조방법,(3) The method for producing noodles according to (1) or (2), further comprising water-soluble hemicellulose,
(4) 수용성 헤미 셀룰로오스는 콩류 유래인, (3) 기재의 면류의 제조방법,(4) Water-soluble hemicellulose is derived from soybean, (3) the production method of noodles,
(5) 콩류는, 대두 또는 완두콩인, (4) 기재의 면류의 제조방법,(5) The legumes are soybeans or peas, (4) the production method of noodles,
(6) (1) 내지 (5) 중 어느 한 항에 기재된 제조방법에 의해 제조되는 면류,(6) The noodle produced by the production method according to any one of (1) to (5)
(7) 원료 분말 1g 당 1 내지 20unit의 1,3-위치 특이성을 갖는 리파아제 및 원료 분말 1g 당 0.1 내지 5unit의 아밀라아제를 반죽에 첨가하는 것을 특징으로 하는, 면류의 분리 개량방법,(7) A process for separating and improving noodles, characterized by adding 0.1 to 5 units of amylase per 1 g of raw material powder to lipase having 1,3-position specificity of 1 to 20 units per 1 g of raw material powder,
(8) 원료 분말 1g 당 1 내지 20unit의 1,3-위치 특이성을 갖는 리파아제 및 원료 분말 1g 당 0.1 내지 5unit의 아밀라아제를 포함하는 것을 특징으로 하는, 면용 분리 개량제, 이다.(8) lipase having 1,3-position specificity of 1 to 20 units per gram of raw material powder and 0.1 to 5 units of amylase per gram of raw material powder.
본 발명에 따르면, 1,3-위치 특이성을 갖는 리파아제 및 아밀라아제를 면에 첨가함으로써, 보존한 후에도 면류의 분리성을 개량할 수 있는 것과 동시에, 면의 탄력이나 찰기를 유지하는 효과도 가진다. 또한, 수용성 헤미 셀룰로오스를 병용함으로써 분리성이 더욱 개량된다.According to the present invention, lipase and amylase having 1,3-position specificity are added to the cotton to improve the separability of noodles even after preservation, and also maintain the elasticity and stickiness of the cotton. In addition, separability is further improved by using water-soluble hemicellulose in combination.
이하, 본 발명을 구체적으로 설명한다.Hereinafter, the present invention will be described in detail.
(면류)(noodles)
본 발명의 면류(Noodles)는 건면, 생면, 프라이(fried)면, 열풍건조 즉석면, 동결건조 즉석면 등이며, 구체적으로는 소바, 우동, 키시멘(子), 중화면, 파스타, 마카로니, 소면, 포(pho), 한국 냉면, 당면(春雨) 등이 있다.The noodles of the present invention are noodles, noodles, fried noodles, hot-air dried instant noodles, freeze-dried instant noodles, and the like. Specifically, noodles such as buckwheat noodles, udon noodles, kishimen noodles, Chinese noodles, pasta, macaroni, Pho, Korean noodles, and vermicelli.
이러한 면류에는, 가정에서 조리되는 것을 시작으로, 그 자리에서 먹는 것을 목적으로 하는 최종 상품 형태의 가공식품이나, 식사 시 조리가 필요한 반제품이 포함되고, 상온, 냉장, 냉동, 빙온 등의 방법으로 시장에 유통되고 있는 식품이 포함된다. Such noodles include processed foods of the final product type intended for eating on the spot, semi-finished products requiring cooking at the time of eating, starting from being cooked at home, and stored at room temperature, refrigeration, freezing, And the like.
(리파아제)(Lipase)
본 발명에서 사용하는 1,3-위치 특이성을 갖는 리파아제(Lipase)는, 세균, 곰팡이, 효모, 사상균, 방선균 등의 미생물 유래의 리파아제 외에, 췌장, 간장(肝臟), 우유, 타액 등의 동물 유래의 리파아제, 쌀겨, 피마자, 엔 보리, 밀, 유채씨, 땅콩 등의 식물 유래의 리파아제 등이 적용 가능하다. 한편, 상술한 리파아제는 정제된 리파아제 것 이외에, 이들 리파아제를 포함하는 조효소이어도 좋고, 예를 들면, 미생물 유래 효소의 경우에는 미생물을 배양한 배지 성분을 포함하는 리파아제 함유 수용액, 또는 이를 건조한 것을 포함한다. The lipase having 1,3-position specificity used in the present invention can be used as a lipase derived from a microorganism such as a bacterium, a fungus, a yeast, a filamentous fungus, a actinomycetes and the like as well as an animal origin such as pancreas, liver, milk, Of plant-derived lipase such as lipase, rice bran, castor, enbory, wheat, rapeseed, and peanut. On the other hand, the above-mentioned lipase may be a coenzyme containing the lipase in addition to the purified lipase. For example, in the case of an enzyme derived from a microorganism, a lipase-containing aqueous solution containing a medium component in which a microorganism is cultured, .
1,3-위치 특이성 리파아제의 기원은 췌장, 간장, 우유, 타액 등과 같은 동물 유래의 리파아제, 미생물에서는, 아스페르길루스(Aspergillus)속, 리조무코르(Rhizomucor)속, 더모마이시스(Theremomyces)속, 리조푸스(Rhizopus)속, 크로모박테리움(Chromobacterium)속, 무코르(Mucor)속, 사카로마이콥시스(Saccharomycopsis)속, 파이코마이시스(Phycomyces)속, 알칼리게네시스(Alcaligenes)속, 슈도모나스(Pseudomonas)이 바람직하다. 본 발명의 리파아제는, 리파아제 제제의 고형분 당 지질 분해 활성을 높이기 위해서, 예를 들면 일반적인 효소 정제법, 또는 리파아제를 고분비 생산시키는 것에 의해, 리파아제의 순도, 정제도를 높인 것을 이용하는 것이 바람직하다. 또한, 리파아제로서 유전자 조작된 것도 사용될 수 있다. The origins of 1,3-regiospecific lipase is the lipase, microorganisms of animal origin, such as the pancreas, liver, milk, saliva, Aspergillus (Aspergillus) in, Lee jomu Accor (Rhizomucor), An My Dermo System (Theremomyces) The genus Rhizopus , the genus Chromobacterium , the genus Mucor , the genus Saccharomycopsis , the genus Phycomyces , the genus Alcaligenes , a Pseudomonas (Pseudomonas) are preferred. It is preferable that the lipase of the present invention be obtained by increasing the purity and purification degree of the lipase by, for example, general enzyme purification method or production of lipase by high secretion in order to enhance the lipolytic activity per lipid of the lipase preparation. In addition, genetically engineered lipases can also be used.
리파아제 중 1,3-위치 특이성을 가지는지 아닌지는, 예를 들면 "리파아제의 위치 특이성을 정량적으로 표현하는 지수" (발효 공학 회지 : No 5, 405-421 (1988)) 에 기재된 시험 방법에 의해 평가할 수 있다. 시판되고 있는 효소로 말하면, 리파아제A "아마노(Amano)" 6, 리파아제G "아마노"50, 리파아제DF "아마노"15(Amano Enzyme Inc), 리파아제A-10D (Nagase ChemteX Corporation), 베이크자임(Bakenzyme) L80000B (DSM) 등이 예시된다. 한편, 1,3-위치 특이성을 갖지 않는 리파아제로 예를 들면, 리파아제AY"아마노" 30D-K (아마노엔자임), 리파아제MY, 리파아제OF (Meito Sangto Co,. Ltd.) 등이 있다. Whether or not 1,3-position specificity in lipase is judged by the test method described in, for example, "Index expressing quantitatively the position specificity of lipase" (Fermentation Engineering Journal: No. 5, 405-421 Can be evaluated. The commercially available enzymes include lipase A "Amano" 6, lipase G "Amano" 50, lipase DF "Amano Enzyme Inc.", lipase A-10D (Nagase ChemteX Corporation), Bakenzyme ) L80000B (DSM). On the other hand, lipases having no 1,3-position specificity include, for example, lipase AY "Amano" 30D-K (Amano Enzyme), lipase MY, and lipase OF (Meito Sangto Co, Ltd.).
본 발명의 1,3-위치 특이성을 갖는 리파아제는 면에 사용되는 원료 분말 1g에 대해 1 내지 20 unit의 범위에서 배합하는 것이 바람직하고, 더욱 바람직하게는, 2 내지 15 unit의 범위에서 배합한다. 1,3-위치 특이성을 갖는 리파아제의 함량이 너무 낮으면 면의 분리성이 불충분하게 되고, 1,3-위치 특이성을 갖는 리파아제의 함량이 너무 높으면 지방산 유래의 나쁜 풍미가 발생하는 경우가 있다. The lipase having 1,3-position specificity of the present invention is preferably blended in the range of 1 to 20 units, and more preferably 2 to 15 units, per 1 g of the raw material powder used for the cotton. If the content of the lipase having 1,3-position specificity is too low, the separability of the surface becomes insufficient. If the content of the lipase having 1,3-position specificity is too high, bad flavor derived from fatty acid may occur.
또한, 본 발명에서 말하는 1,3-위치 특이성을 갖는 리파아제의 1 unit은 JIS0601-1995 "공업용 리파아제의 활성측정방법"에 나타난 방법에 의해 측정한 것이다. 즉, 1unit이란, 특정 조건 하에서 기질(올리브유)에서 1분간 1μmol의 지방산을 유리시키는 효소량을 나타낸다.Furthermore, 1 unit of lipase having 1,3-position specificity according to the present invention is measured by the method shown in JIS0601 -1995 "method measuring the activity of the industrial lipase". That is, 1 unit represents the amount of enzyme which liberates 1 μmol of fatty acid in the substrate (olive oil) for 1 minute under a specific condition.
리파아제가 면의 분리에 기여하는 이유로서, 면 반죽에서 밀에 함유되는 유지를 분해하여 모노글리세라이드(monoglyceride) 및 디글리세라이드(diglyceride)가 생성하는 것을 들 수 있다. 생성된 모노글리세라이드 및 디글리세라이드는 면 반죽의 전분 등에 작용하여 분리성을 개선하고 있다고 추측된다. 1,3-위치 특이성을 갖는 리파아제에서는, 2 위치의 지방산이 절단되지 않아, 모노글리세라이드 상태에서 반응을 멈추는 것이 많지만, 램덤으로 작용하는 리파아제는 모든 위치의 지방산이 절단되기 때문에 분리가 일어나기 어렵고, 지방산 유래의 불쾌한 향이 나타나기 쉬운 것으로 추측된다.The reason why lipase contributes to the separation of cotton is that the fat contained in the wheat is decomposed in the cotton dough to produce monoglyceride and diglyceride. The resulting monoglyceride and diglyceride are supposed to act on the starch of the cotton dough and the like to improve the separability. In the lipase having 1,3-position specificity, the fatty acid at the 2-position is not cleaved and the reaction is stopped in the monoglyceride state. However, the lipase which acts randomly is difficult to separate because fatty acids at all positions are cleaved, It is presumed that an unpleasant fragrance derived from a fatty acid tends to appear.
(아밀라아제)(Amylase)
본 발명에서 사용하는 아밀라아제(Amylase)로는, 소·돼지의 췌장 등의 동물 유래의 것, 맥아, 보리, 밀, 고구마, 대두 등의 식물 유래의 것, 세균, 곰팡이, 효모, 사상균, 방선균 등의 미생물 유래의 것 등이 예시된다. 또한, 상술한 아밀라아제는 정제된 것 이외에, 이러한 아밀라아제를 포함하는 조효소이어도 좋고, 예를 들면 미생물 유래 효소의 경우 미생물을 배양한 배지 성분을 포함하는 아밀라아제 함유 수용액, 또는 이를 건조한 것도 포함한다. 미생물 유래의 경우, 바실러스(Bacillus)속, 아스페르길루스(Aspergillus)속 유래의 아밀라아제를 들 수 있고, α-아밀라아제, β-아밀라아제, 글루코아밀라아제(glucoamylase) 등을 예시할 수 있으며, 그 중에서도 α-아밀라아제가 가장 바람직하다. 이들은 단독으로 또는 2종 이상 혼합해 이용할 수 있다.Examples of the amylase used in the present invention include those derived from animals such as pancreas of small or pigs, those derived from plants such as malt, barley, wheat, sweet potato and soybean, bacteria, fungi, yeast, And those derived from microorganisms. The amylase described above may be a coenzyme containing such an amylase in addition to the purified one. For example, in the case of an enzyme derived from a microorganism, an amylase-containing aqueous solution containing a medium component in which a microorganism is cultured, or a dried product thereof. Examples of microorganism-derived microorganisms include amylase derived from genus Bacillus or Aspergillus, and examples thereof include? -Amylase,? -Amylase and glucoamylase. Of these,? - Amylase is most preferred. These may be used alone or in combination of two or more.
아밀라아제는 면에 사용되는 원료 분말 1g에 대하여 0.1 내지 5 unit/g의 범위에서 배합하는 것이 바람직하고, 더욱 바람직하게는, 0.2 내지 3 unit/g의 범위에서 배합한다. 아밀라아제의 함량이 너무 낮으면, 면의 분리성이 불충분하게 되고, 아밀라아제의 함량이 너무 높으면, 면이 너무 부드러워지는 경우가 있다. The amount of the amylase is preferably in the range of 0.1 to 5 unit / g, more preferably 0.2 to 3 unit / g, based on 1 g of the raw material powder used for the cotton. If the content of the amylase is too low, the separability of the surface becomes insufficient, and if the content of the amylase is too high, the surface may become too soft.
한편, 본 발명에서 말하는 아밀라아제 1 유닛(unit)이란, 기존 첨가물 자주 규격에 기재된 "α-아밀라아제의 활성 측정 방법"에 나타난 방법에 의해 측정한 것이다. 즉, 1 유닛(unit)이란 감자 전분을 기질로 하고, 정해진 조건에서 시험할 때 1분간 전분의 요오드에 의해 청색을 10% 감소시키는 효소량을 나타낸다. On the other hand, the term "unit of amylase" used in the present invention is measured by the method described in "Method for measuring the activity of α-amylase" described in existing additive reference materials. That is, 1 unit represents the amount of enzyme which reduces 10% of the blue color by starch iodine for 1 minute when the potato starch is used as a substrate and tested under specified conditions.
본 발명에서는, 원료분말은 면 반죽에 사용하는 것으로, 밀가루를 사용하는 것이 많지만, 보리, 호밀, 대두, 그 외의 콩류, 메밀, 쌀, 옥수수 등의 곡분을 포함해도 좋다. 또한, 전분, 가공처리분말, 당류, 수용성 식이섬유, 불용성 식이섬유, 두유분말, 대두 단백질, 유 단백질, 유장(乳漿), 난백, 난황, 콜라겐 등의 단백질, 및 단백질 분해물, 및 펩타이드를 포함해도 좋다. 본 발명에서 이들은 원료 분말이 된다. In the present invention, the raw material powder is used for kneading cotton, and many wheat flour is used. However, it may include barley, rye, soybean, other legumes, buckwheat, rice, corn and other flour. Also included are proteins such as starch, processed powders, saccharides, water soluble dietary fiber, insoluble dietary fiber, soybean milk powder, soy protein, milk protein, whey protein, egg white, egg yolk, collagen and proteolytic products and peptides Maybe. In the present invention, these are raw material powders.
(수용성 헤미셀룰로오스)(Water-soluble hemicellulose)
수용성 헤미셀룰로오스는 유량(油糧)종자(야자, 코코넛, 옥수수, 목화씨 등) 또는 곡류(쌀, 밀 등), 콩(대두, 팥, 완두콩 등)을 원료로 하여 얻을 수 있지만, 용해성이나 공업성 면에서, 본 발명에서는 콩류 유래가 바람직하고, 보다 바람직하게는 대두나 완두콩, 더욱 바람직하게는 대두이며, 그중에서도 자엽(子葉) 유래인 것이 바람직하다.Water-soluble hemicellulose can be obtained from raw materials such as oil seeds (palm, coconut, corn, cottonseed) or cereals (rice, wheat), soybeans (soybeans, red beans, peas etc.) In the present invention, soybean is preferably derived from soybean, more preferably soybean or pea, more preferably soybean, among which cotyledon is preferred.
수용성 헤미셀룰로오스는 헤미셀룰로오스를 포함하는 원료로부터 물 추출이나 열수 추출하는 방법, 산, 알칼리 조건 하에서 가열 추출하는 방법, 효소 분해에 의한 추출 방법 등을 이용해 얻을 수 있다.The water-soluble hemicellulose can be obtained by a method of extracting water or hot water from a raw material containing hemicellulose, a method of heating extraction under acidic or alkaline conditions, and an extraction method by enzymatic decomposition.
원료로서 대두를 이용한 경우의 수용성 헤미셀룰로오스 제조법의 바람직한 일례를 나타내면 이하와 같다. 이들의 원료를 산성 내지 알칼리성 조건 하, 바람직하게는 각 단백질의 등전점 부근의 pH, 바람직하게는 80℃ 이상 130℃ 이하, 보다 바람직하게는 100℃ 초과 130℃ 이하에서 가열분해 하고, 원심분리 등으로 수용성 획분을 분획한 후, 그대로 건조하거나, 예를 들면, 활성탄 처리 또는 수지 흡착처리 또는 에탄올 침전 처리하여 소수성 물질 또는 저분자 물질을 제거하여 건조하는 것에 의해서, 수용성 헤미셀룰로오스를 얻을 수 있다. A preferred example of the method for producing a water-soluble hemicellulose in the case of using soybean as a raw material is as follows. These raw materials are heated and decomposed under acidic to alkaline conditions, preferably at a pH near the isoelectric point of each protein, preferably 80 ° C or more and 130 ° C or less, more preferably 100 ° C or more and 130 ° C or less, Soluble hemicellulose can be obtained by fractionating the water-soluble fraction, drying it as it is, removing the hydrophobic substance or the low molecular substance by, for example, an activated carbon treatment, a resin adsorption treatment or an ethanol precipitation treatment and drying.
본 발명에 있어서의 수용성 헤미셀룰로오스의 바람직한 첨가량은 특별히 제한되는 것은 아니지만, 면에 사용되는 분말을 기준으로 0.01 내지 5중량% 이며, 더욱 바람직하게는 0.05 내지 3중량% 이다. The amount of the water-soluble hemicellulose to be added in the present invention is not particularly limited, but it is 0.01 to 5% by weight, more preferably 0.05 to 3% by weight, based on the powder used for the cotton.
(단백질)(protein)
본 발명에서 이용되는 단백질은 대두, 완두콩, 녹두 등의 콩류, 밀, 난백을 원료로 하는 열 응고성 단백질을 사용하는 것이 바람직하고, 보다 바람직하게는 대두, 완두콩, 녹두 등의 콩류의 단백질이다. 또한, 비용을 억제할 수 있는 점, 식감에 더욱 찰기와 탄력을 부여할 수 있다는 점에서 대두 단백질이 더욱 바람직하다. 대두 단백질로서, 분리 대두 단백질, 알칼리 토류금속 결합 분리 대두 단백질, 농축 대두 단백질, 전지두유, 전지두유 분말, 탈지두유, 탈지두유 분말, 탈지두유 또는 탈지두유 분말의 알칼리 토류금속 결합물 등을 예시할 수 있다. 이들 단백질은 1종 이상을 병용하여 사용할 수 있다.The protein used in the present invention is preferably a thermostable protein derived from soybean, pea, mung bean, wheat, wheat or egg white, and more preferably soybean protein, pea, mung bean, and the like. In addition, the soy protein is more preferable because it can suppress the cost and can impart more firmness and elasticity to the texture. Examples of the soybean protein include alkaline earth metal binders of soybean protein isolate, alkaline earth metal binding cleavage soybean protein, concentrated soybean protein, cell soymilk, cell soymilk powder, defatted soybean milk, defatted soybean milk powder, defatted soybean milk or defatted soybean milk powder . One or more of these proteins can be used in combination.
단백질의 제조법은 식품 제조에서 일반적인 방법이라면 특별히 제한되지 않으며, 가수(加水)추출, 등전점침전, 막 분리, 수세(水洗), 정제 및 탈당(脫糖) 등의 공정을 거쳐 제조된다. 필요에 따라서 이러한 단백질은 산, 알칼리, 효소 등으로 분해된 것을 사용하는 것도 가능하다.The method for producing a protein is not particularly limited as long as it is a general method in food production and is manufactured through processes such as hydrolysis extraction, isoelectric point precipitation, membrane separation, washing with water, purification and desalting. If necessary, these proteins may be decomposed with an acid, an alkali, an enzyme, or the like.
본 발명의 면류의 반죽에는 1,3-위치 특이성을 갖는 리파아제와 아밀라아제를 배합하는 것이 필수이지만, 수용성 헤미셀룰로오스 이외에 적절하게, 면질(麵質)을 조정하는 등의 목적에 의해 다른 첨가제와 병용할 수 있다. 다른 첨가제로서, 글루코오스 및 프럭토오스 등의 단당류, 수크로오스(sucrose), 말토오스(maltose), 락토오스(lactose), 라피노오스(raffinose), 말토트리오스(maltotriose), 트레할로스(trehalose), 스타키오스(stachyose), 말토테트라오스(maltotetraose)와 같은 올리고당, 및, 당 알코올, 덱스트린(dextrin), 후노란(funoran), 한천, 카라기난(carrageenan), 퍼셀러랜(furcelleran), 타마린드 종자 다당류(tamarind seed polysaccharides), 타라 검(tara gum), 카라야 검(karaya gum), 트라가칸트 검(tragacanth gum), 펙틴, 잔탄검(xanthan gum), 알긴산 나트륨, 구아검(guar gum), 가티 검(Gati gum), 로커스트빈검(locust bean gum), 풀루란(pullulan), 겔란 검(gellan gum), 아라빅 검(arabic gum), 커드란(curdlan), 결정 셀룰로오스, 메틸 셀룰로오스, 하이드록시프로필 메틸 셀룰로오스, 히알루론산, 사이클로덱스트린, 키토산, 카르복시 메틸 셀룰로오스(CMC), 알긴산프로필렌글라이콜에스테르, 가공 전분 등 각종 전분류 등의 다당류나, 이들 다당류의 가수분해물, 우유, 대두, 계란, 밀 등의 단백질, 젤라틴, 가용성 콜라겐, 칼슘 강화제 등의 염류, 아세트산, 구연산, 젖산, 아디프산 및 글루콘산과 같은 산미료, 모노 글리세린 지방산 에스테르, 유기산 모노 글리세린 지방산 에스테르, 레시틴 및 스테아로일 락테이트 염(stearoyl lactate salt)의 군에서 선택되는 1종 이상의 유화제를 병용하면, 면의 분리성과 면질이 개량되기 때문에 바람직하다. 그 외에, 수크로오스 지방산 에스테르(sucrose fatty acid ester), 폴리글리세린 지방산 에스테르(polyglycerol fatty acid ester), 소르비탄 지방산 에스테르(sorbitan fatty acid ester), 프로필렌 글리콜 지방산 에스테르(propylene glycol fatty acid ester), 폴리 소르베이트(polysorbate) 등의 유화제를 병용할 수도 있다. It is essential to blend lipase having 1,3-position specificity with amylase in the noodle of the present invention. However, in addition to water-soluble hemicellulose, it can be used in combination with other additives for the purpose of adjusting the quality of the surface, have. Other additives include monosaccharides such as glucose and fructose, sucrose, maltose, lactose, raffinose, maltotriose, trehalose, stachyose, stachyose, maltotetraose, and oligosaccharides such as sugar alcohols, dextrin, funoran, agar, carrageenan, furcelleran, tamarind seed, polysaccharides, tara gum, karaya gum, tragacanth gum, pectin, xanthan gum, sodium alginate, guar gum, Gati gum, locust bean gum, pullulan, gellan gum, arabic gum, curdlan, crystalline cellulose, methylcellulose, hydroxypropylmethylcellulose, hydroxypropylmethylcellulose, Hyaluronic acid, cyclodextrin, chitosan, carboxymethylcellulose Polysaccharides such as various starches such as lactose (CMC), propylene glycol esters of alginic acid and processed starches, hydrolysates of these polysaccharides, proteins such as milk, soybean, egg and wheat, gelatin, soluble collagen, Salts, acetic acid, citric acid, lactic acid, acidic acid such as adipic acid and gluconic acid, monoglycerin fatty acid ester, organic acid monoglycerin fatty acid ester, lecithin and stearoyl lactate salt When an emulsifier is used in combination, the separability and surface quality of the surface are improved. In addition, there may be mentioned sucrose fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, propylene glycol fatty acid esters, polysorbate esters, an emulsifier such as polysorbate may be used in combination.
1,3-위치 특이성을 갖는 리파아제 및 아밀라아제, 더욱 바람직하게는 수용성 헤미셀룰로오스를 면류에 첨가하는 방법은 특히 한정되지 않지만, 조리면, 즉석면 또는 파스타의 면 반죽에 반죽하는 방법으로는, (1) 밀가루 등에 분말을 혼합하여 첨가하는 방법, (2) 소금이나 견수, 조미료와 함께 반죽물에 용해시켜 첨가하는 방법이 예시될 수 있다. 이러한 방법은 제조 라인, 판매 형태 등 개개의 식품 특성에 의해 선택된다.The method of adding the lipase and the amylase having 1,3-position specificity, more preferably the method of adding water-soluble hemicellulose to the noodle is not particularly limited, but the method of kneading on the cooked surface, A method of adding powders to flour and the like, and (2) a method of dissolving in a kneaded water together with salt, cold water, and seasoning. This method is selected according to the individual food characteristics such as the production line and the sales form.
1,3-위치 특이성을 갖는 리파아제와 아밀라아제, 더욱 바람직하게는 수용성 헤미 셀룰로오스를 면 반죽 중에 효율적으로 작용시키기 위해, 면 반죽의 배합에 분리 개량제와 유지를 동시에 첨가하는 것이 가능하다. 첨가하는 유지는 특히 제한되는 것이 아니고, 동식물 유래의 유지를 사용할 수 있다. 돼지기름, 쇠기름, 유지방, 난황, 콩기름, 유채씨유, 카카오유, 쌀겨유, 해바라기씨유, 콘유, 면실유, 팜유, 야자유, 참기름, 올리브유, 들기름 등이 예시된다. 또는, 이들 유지를 경화, 분별, 에스테스 교환한 것도 사용할 수 있다. 이들 중 1종 또는 2종 이상의 유지를 조합하여 사용할 수 있다. It is possible to simultaneously add a separation improver and a preservative to the combination of cotton dough in order to efficiently operate lipase and amylase having 1,3-position specificity, and more preferably water-soluble hemicellulose, in cotton dough. The oil to be added is not particularly limited, and an animal or vegetable oil-based oil can be used. Soybean oil, rice bran oil, sunflower oil, konjoo oil, palm oil, palm oil, sesame oil, olive oil, perilla oil and the like. Alternatively, those obtained by hardening, fractionating and / or exchanging these oils may be used. One or more of these may be used in combination.
1,3-위치 특이성을 갖는 리파아제 및 아밀라아제, 더욱 바람직하게는 수용성 헤미셀룰로오스를 면류에 첨가하는 방법에 의해 얻은 면류는, 양호한 분리성을 가지고 있다. 따라서, 본 발명에 의해 얻은 면류는 매우 먹기 쉽고 맛있다. 또한, 예를 들면 조리면의 경우, 제조 후 1~2일 냉장 보관한 후에도 이러한 분리성이 유지되고, 면끼리의 결착이 현저하게 억제되어 면의 탄력이나 찰기가 유지되고 있다. 또한, 양호한 분리성이 부여됨으로써, 면류의 제조에 있어 혼합이 용이하고, 균열이 일어나기 어려워지는 등의 뛰어난 가공적정(加工適正)을 갖는다. The noodles obtained by the method of adding lipase and amylase having 1,3-position specificity, and more preferably, adding water-soluble hemicellulose to the noodle have good separability. Therefore, the noodles obtained by the present invention are very easy to eat and delicious. Further, for example, in the case of a cooked surface, such separability is maintained even after refrigerated storage for 1 to 2 days after the production, and binding of the surfaces is remarkably suppressed, so that elasticity and stickiness of the surface are maintained. In addition, by having good separability, it is possible to mix easily in the production of the noodle stream, and to have excellent processability such as less cracking.
(면용의 분리 개량제)(Separation improver for cotton)
본 발명의 면용 분리 개량제는 원료 분말 1g 당 1 내지 20 unit의 1,3-위치 특이성을 갖는 리파아제와 원료 분말 1g 당 0.1 내지 5 unit의 아밀라아제 를 포함하는 것을 특징으로 한다. 수용성 헤미셀룰로오스를 추가로 포함하는 경우, 면의 분리성이 향상되는 기능이 높아지므로 바람직하다. The cottonseed improving agent of the present invention is characterized by comprising 1 to 20 units of lipase having 1,3-position specificity per 1 g of the raw material powder and 0.1 to 5 units of amylase per 1 g of the raw material powder. When water-soluble hemicellulose is further contained, the function of improving the surface separability is enhanced, which is preferable.
본 분리 개량제의 면류에 대한 첨가량은, 면의 종류나 분리 개량제의 배합에 따라 다르지만, 밀가루 등의 원료 분말의 중량에 대해 바람직하게는 0.001 내지 5중량%, 보다 바람직하게는 0.002 내지 3중량%가 예시된다.The addition amount of the present separation improving agent to the noodle is preferably 0.001 to 5% by weight, more preferably 0.002 to 3% by weight based on the weight of the raw material powder such as wheat flour, etc., .
실시예Example
이하에서는, 실시예를 기재한다.Hereinafter, examples will be described.
한편, 예 중의 부(部) 및 %는 중량 기준을 의미한다.In the examples, parts and% in the examples mean weight basis.
(실시예 1)(Example 1)
밀가루 600g, 타피오카 전분 390g, 밀 글루텐 10g의 혼합물에 식염 20g, 1,3-위치 특이성을 갖는 리파아제로서, 리파아제A "아마노" 6 (Amano Enzyme: 60,000 unit/g) 0.1g, 아밀라아제로서 Sumizyme AS (Shin Nihon Chemical Industry Co., Ltd.: 1,500 unit/g)을 물 450g에 용해시킨 반죽물을 첨가하여 혼련(混練)하여 면 반죽을 만들었다. 반죽을 압연(壓延)하고, #20의 절단 날로 잘라 중화면을 얻어, 실시예 1로 하였다. 중화면을 80초간 삶은 후, 물로 헹군 다음, 100g씩 귀갑용기(龜甲容器)에 소분하여 4℃에서 2일간 보관하였다. 이 중화면에 대해, 분리성, 면질(탄력성, 경도)를 평가하였다. 20 g of sodium chloride was added to a mixture of 600 g of flour, 390 g of tapioca starch and 10 g of wheat gluten, 0.1 g of lipase A "Amano Enzyme" (60,000 unit / g) as a lipase having 1,3-position specificity and 0.1 g of Sumizyme AS Manufactured by Shin Nihon Chemical Industry Co., Ltd.: 1,500 unit / g) dissolved in 450 g of water was added and kneaded to prepare a cotton dough. The dough was rolled, cut with a cutting blade # 20 to obtain a neutralized surface, and the result was regarded as Example 1. The neutralized surface was boiled for 80 seconds, rinsed with water, and then subdivided into 100 grams of a glove box (container) and stored at 4 ° C for 2 days. Separability and surface quality (elasticity and hardness) of the neutralized surface were evaluated.
(실시예 2)(Example 2)
실시예 1에 기재된 방법 중, 1,3-위치 특이성을 갖는 리파아제로서 리파아제 DF "아마노" 15 (Amano Enzyme Inc. 제조: 150,000unit/g), 아밀라아제로서 Biozyme A (Amano Enzyme Inc.: 7500unit/g)을 이용하는 것 이외에는 마찬가지의 방법으로 중화면을 만들었다.Among the methods described in Example 1, lipase DF "Amano" 15 (Amano Enzyme Inc., 150,000 units / g) as the 1,3-position specific lipase, Biozyme A (Amano Enzyme Inc .: 7500 units / g ) Was used as the neutralizing agent.
(비교예 1)(Comparative Example 1)
실시예 1에 기재된 방법 중, 리파아제를 사용하지 않은 것 이외에는 마찬가지의 방법으로 중화면을 조제하였다.Among the methods described in Example 1, a neutralized surface was prepared by the same method except that no lipase was used.
(비교예 2)(Comparative Example 2)
실시예 1에 기재된 방법 중, 1,3-위치 특이성을 갖는 리파아제로서 리파아제 A "아마노" 6 (Amano Enzyme Inc. 제조: 60,000 unit/g)을 0.1g 사용하는 것 이외에는 마찬가지의 방법으로 중화면을 조제하였다. Except that 0.1 g of Lipase A "Amano" 6 (manufactured by Amano Enzyme Inc., 60,000 unit / g) was used as a lipase having 1,3-position specificity among the methods described in Example 1, Lt; / RTI >
(비교예 3)(Comparative Example 3)
실시예 1에 기재된 방법 중, 1,3-위치 특이성을 갖는 리파아제로서 리파아제 DF "아마노" 15 (Amano Enzyme Inc. 제조: 150,000unit/g)을 사용하는 것 이외에는 마찬가지의 방법으로 중화면을 조제하였다. A neutralized surface was prepared in the same manner as in Example 1 except that Lipase DF "Amano" 15 (manufactured by Amano Enzyme Inc., 150,000 units / g) was used as a lipase having 1,3-position specificity .
(비교예 4)(Comparative Example 4)
실시예 1에 기재된 방법 중, 아밀라아제로 Biozyme A (Amano Enzyme Inc.)을 사용하는 것 이외에는 마찬가지의 방법으로 중화면을 조제하였다. Among the methods described in Example 1, a neutralized surface was prepared by the same method except that Biozyme A (Amano Enzyme Inc.) was used as the amylase.
(비교예 5)(Comparative Example 5)
실시예 1에 기재된 방법 중, 효소로서 프로테아제인 Sumizyme LPL (Shin Nihon Chemical Industry Co., Ltd.: 500,000 unit/g)만을 사용한 것 이외에는 마찬가지의 방법으로 중화면을 조제하였다. A neutralized surface was prepared in the same manner as in Example 1 except that only the enzyme Sumizyme LPL (Shin Nihon Chemical Industry Co., Ltd., 500,000 unit / g) was used as the enzyme.
(비교예 6)(Comparative Example 6)
실시예 1에 기재된 방법 중, 리파아제로서 1,3-위치 특이성을 갖지 않는 리파아제 AY "아마노" 30 (Amano Enzyme Inc. 제조: 30,000 unit/g), 아밀라아제로서 Sumizyme AS를 사용하는 것 이외에는 마찬가지의 방법으로 중화면을 조제하였다. Except that lipase AY "Amano" 30 (Amano Enzyme Inc.: 30,000 unit / g) having no 1,3-position specificity as a lipase and Sumizyme AS as an amylase were used in the method described in Example 1, To prepare a neutralized surface.
(비교예 7)(Comparative Example 7)
실시예 1에 기재된 방법 중, 리파아제로서 1,3-위치 특이성을 갖지 않는 리파아제 MY (Meito Sangyo Co., Ltd. 제조: 32,000 u/g), 아밀라아제로서 Sumizyme AS를 사용하는 것 이외에는 마찬가지의 방법으로 중화면을 조제하였다. Among the methods described in Example 1, lipase MY (manufactured by Meito Sangyo Co., Ltd., 32,000 u / g) having no 1,3-position specificity was used as the lipase, and Sumizyme AS was used as the amylase, A neutralized surface was prepared.
○면의 평가 방법○ Evaluation method of cotton
2일간 보존한 면에, 면 국물을 첨가하고, 분리된 상태를 눈으로 관찰하였다. 분리성이 매우 좋은 것을 5점, 분리성이 매우 나쁜 것을 1점으로서 5단계로 평가하고, 패널 6명의 평가의 평균을 평가점으로 하였다. 평균값이 3.0점 이상이면 합격으로 하였다. Cotton juice was added to the surface preserved for 2 days, and the separated state was visually observed. Five points for very good separability and one point for very poor separability were evaluated in five levels, and the evaluation point was the average of the evaluation of six panelists. If the average value is 3.0 or more, the acceptance is made.
면의 식감에 대해서는, 탄력과 찰기에 대하여, 숙련된 패널 6명으로 평가하였다. 탄력, 찰기 모두 매우 좋은 것을 5점, 매우 나쁜 것을 1점으로 하여 5단계로 평가하고, 평균점을 산출했다. 평균값이 3.0점 이상이면, 합격으로 하였다. Regarding the texture of the surface, the elasticity and the stickiness were evaluated by six skilled panelists. 5 points for very good and 2 points for very bad ones. If the average value is 3.0 or more, the acceptance is made.
면의 풍미에 대해서는, 이상한 풍미를 느끼는 것을 경우 ⅹ, 그 이외를 ○로 하고, ○의 것을 합격으로 하였다.Regarding the flavor of the surface, in the case of feeling strange flavor x, the others were evaluated as ○, and those of ○ were regarded as acceptable.
면의 분리성, 식감 및 풍미 모두 합격인 것을 종합 평가에 ○로 하여 합격으로 했다. 면의 분리성, 식감, 풍미 중 어느 하나에서도 불합격인 것이 있는 경우, 종합 평가는 ⅹ로서 불합격으로 했다. It was accepted as ○ in the comprehensive evaluation that the separability of the cotton, the texture and the flavor were both acceptable. In the case where any one of the detachability, texture, and flavor of the face is not acceptable, the composite evaluation is determined to be rejected as x.
(리파아제의 활성 측정방법)(Method for measuring activity of lipase)
리파아제의 가수 분해 활성의 측정에 대해서는 JIS0601-1995 "공업용 리파아제의 활성 측정방법"에 따라 수행했다. Measurement of the hydrolytic activity of lipase was carried out in accordance with JIS0601 -1995 "Method for measuring activity of industrial lipase ".
(아밀라아제의 활성 측정방법)(Method for measuring the activity of amylase)
아밀라아제의 가수 분해 활성의 측정에 대해서는 기존 첨가물의 자주규격에 기재된 "α-아밀라아제의 활성 측정법"에 따라 수행했다.The measurement of the hydrolytic activity of amylase was carried out in accordance with the "method for measuring the activity of alpha -amylase "
실시예 1~2 및 비교예 1-7의 평가 결과를 표1에 나타내었다.The evaluation results of Examples 1 and 2 and Comparative Example 1-7 are shown in Table 1.
1,3-위치 특이성을 갖는 리파아제와 아밀라아제를 병용한 실시예 1, 2에서는 매우 양호한 분리와 식감을 얻을 수 있고, 1,3-위치 특이성을 갖는 리파아제나 아밀라아제를 각각 단독으로 첨가한 것보다도 분리성이 양호해졌다. 반면, 1,3-위치 특이성을 갖지 않는 리파아제를 사용한 경우, 중화면 (비교예 6 내지 7) 분리성이 불충분하고, 면에서 자극적이고 이상한 풍미가 느껴졌다. In Examples 1 and 2 in which lipase having 1,3-position specificity was used in combination with amylase, very good separation and texture could be obtained, and lipase or amylase having 1,3-position specificity alone Sex has improved. On the other hand, in the case of using lipase having no 1,3-position specificity, the neutralization surface (Comparative Examples 6 to 7) was inadequate in separability and irritating and unusual flavor was felt on the surface.
(리파아제 및 아밀라아제의 첨가량)(Addition amount of lipase and amylase)
(실시예 3 내지 12 및 비교예 7 내지 11)(Examples 3 to 12 and Comparative Examples 7 to 11)
실시예 1에 기재된 방법 중, 1,3-위치 특이성을 갖는 리파아제로서 리파아제 "아마노" 6, 아밀라아제로서 Biozyme A를 표 2에 나타난 것과 같이 첨가하여 얻은 중화면을 실시예 3 내지 12, 비교예 8 내지 11로 하였다. 평가한 결과는 표 2에 나타내었다.Among the methods described in Example 1, lipase "Amano" 6 as a lipase having 1,3-position specificity and Biozyme A as an amylase were added as shown in Table 2 to obtain neutralized surfaces obtained in Examples 3 to 12 and Comparative Example 8 To 11. The evaluation results are shown in Table 2.
1,3-위치 특이성을 갖는 리파아제가 1 내지 20 unit/g 의 범위, 아밀라아제가 0.1 내지 5 unit/g의 범위의 첨가량으로, 면의 양호한 풀림성, 식감, 풍미를 나타내었다. 1,3-위치 특이성을 갖는 리파아제나 아밀라아제의 첨가가 이러한 범위 외인, 비교예 8 내지 11은 풀림성이 나쁘거나, 풀림성이 양호한 경우에도 식감이 나빠졌다. The lipase having 1,3-position specificity was in the range of 1 to 20 unit / g, and the amylase was in the amount of 0.1 to 5 unit / g in the addition amount, thus showing good releasability, texture and flavor of the surface. Comparative Examples 8 to 11, in which the addition of lipase or amylase having 1,3-position specificity was out of this range, had a poor texture even when the loosening property was poor or the loosening property was good.
(실시예 13) 수용성 헤미셀룰로오스의 병용(Example 13) Combination of water-soluble hemicellulose
실시예 1에서, 1,3-위치 특이성을 갖는 리파아제, 아밀라아제와 함께 수용성 헤미셀룰로오스로서 수용성 대두 다당류 (상품명: Soyafibe-S, Fuji Oil Co., Ltd. 제조) 를 1g 첨가하는 것 이외에는 실시예 1과 마찬가지의 방법으로 중화면을 조제하여 실시예 13으로 하였다.Example 1 was repeated except that 1 g of water-soluble soybean polysaccharide (trade name: Soyafibe-S, manufactured by Fuji Oil Co., Ltd.) as water-soluble hemicellulose together with lipase having 1,3-position specificity and amylase was added in Example 1 A neutralized surface was prepared in the same manner as in Example 13.
(실시예 14) 수용성 완두콩 다당류의 병용(Example 14) Combination of water-soluble pea polysaccharide
실시예 1에서, 1,3-위치 특이성을 갖는 리파아제, 아밀라아제와 함께 수용성 헤미셀룰로오스로서 수용성 완두콩 다당류를 1g 첨가한 것 이외에는 실시예 1과 마찬가지로 중화면을 조제하여 실시예 14로 하였다.In Example 1, a neutralized surface was prepared in the same manner as in Example 1, except that 1 g of the water-soluble pea polysaccharide as the water-soluble hemicellulose together with the lipase having the 1,3-position specificity and the amylase was added.
한편, 수용성 완두콩 다당류는 이하의 방법으로 조제했다. On the other hand, the water-soluble pea polysaccharide was prepared by the following method.
완두콩 종자 50kg를 탈피한 후, 5배량의 물을 더하여 24시간 동안 침지하였다. 호모믹서 (5,000rpm, 30분간)에서 종자를 분쇄하여, 단백질 및 전분을 추출하였다. 원심여과기를 사용하여 1,500×g, 20분간 물에 분산되어 있는 단백질이나 전분 등의 성분을 제거하여 섬유질 물질을 회수하였다. 또한, 섬유질에 5배량의 물을 더하여 호모믹서 (3,000rpm, 30분)로 교반하고, 원심 여과 (1500×g, 20분간) 에 의해 섬유질 물질을 회수하였다. 이 조작을 2회 반복하고, 동결 건조하여 10kg의 완두콩 섬유를 얻었다. 완두콩 섬유 80부를 물 920부에 분산시키고, 염산을 이용해 pH 5로 조정한 후, 120℃에서 90분간 가열하여 수용성 완두콩 다당류를 추출하였다. 원심 분리 (5,000rpm, 30분간) 후, 상청(上淸)에 60중량%이 되도록 에탄올을 가하여 수용성 완두콩 다당류를 침전시키고, 90중량%의 함수 에탄올로 정제하여 얻은 침전을 풍건(風乾)하여 수용성 완두콩 다당류를 얻었다. After peeling 50 kg of pea seeds, 5 times of water was added and immersed for 24 hours. Seeds were ground in a homomixer (5,000 rpm, 30 minutes) to extract proteins and starches. The fibrous material was recovered by removing components such as protein and starch dispersed in water at 1,500 x g for 20 minutes using a centrifugal filter. Further, 5-fold amount of water was added to the fiber, stirred with a homomixer (3,000 rpm, 30 minutes), and the fibrous material was recovered by centrifugal filtration (1500 x g, 20 minutes). This operation was repeated twice and lyophilized to obtain 10 kg of pea fiber. 80 parts of pea fibers were dispersed in 920 parts of water, adjusted to pH 5 with hydrochloric acid, and then heated at 120 캜 for 90 minutes to extract the water-soluble pea polysaccharide. After centrifugation (5,000 rpm, 30 minutes), ethanol was added to the supernatant to 60 wt% to precipitate the water soluble pea polysaccharide. The precipitate was purified with 90% by weight aqueous ethanol and air dried to obtain a water- Pea polysaccharide was obtained.
(실시예 15) 대두 단백의 병용(Example 15) Combination of soybean protein
실시예 1에서 1,3-위치 특이성을 갖는 리파아제와 함께 대두 단백으로서 New Fuji Pro SEH (Fuji Oil Co., Ltd. 제조) 1g을 첨가한 것 이외에는 실시예 1과 마찬가지로 하여 중화면을 제조하여 실시예 15로 하였다.A neutralized surface was prepared and treated in the same manner as in Example 1 except that 1 g of New Fuji Pro SEH (manufactured by Fuji Oil Co., Ltd.) was added as a soybean protein together with lipase having 1,3-position specificity in Example 1 Example 15.
실시예 13~15의 평가 결과를 표 3에 나타내었다.The evaluation results of Examples 13 to 15 are shown in Table 3.
1,3-위치 특이성을 갖는 리파아제 및 아밀라아제에 더하여 추가로 수용성 헤미셀룰로오스를 병용하는 경우, 더욱 분리성이 향상되었다. 1,3-위치 특이성을 갖는 리파아제에 대두 단백을 병용하면 식감에 탄력이 부여되었다. Separation properties were further improved when water-soluble hemicelluloses were used in addition to lipase and amylase having 1,3-position specificity. The use of soy protein in lipase with 1,3-position specificity imparted elasticity to the texture.
○ 우동의 평가(실시예 16)○ Evaluation of noodles (Example 16)
밀가루 800g과 타피오카 전분 200g의 혼합물에, 식염 30g, 실시예 13의 배합으로 1,3-위치 특이성을 갖는 리파아제, 아밀라아제 및 수용성 헤미셀룰로오스를 첨가하고, 물 360g에 용해시킨 반죽물을 더하고 혼련하여 면 반죽을 만들었다. 반죽을 압연(壓延)하여 #10 의 절단 날로 잘라 우동으로 하였다. 우동을 10분간 삶아서 물로 헹구고, 100g씩 귀갑용기에 소분하고, 4℃에서 2일 동안 보존했다. To the mixture of 800 g of flour and 200 g of tapioca starch was added 30 g of salt and lipase, amylase and water-soluble hemicellulose having the 1,3-position specificity in the formulation of Example 13, and the kneaded product dissolved in 360 g of water was added, . The dough was rolled and cut into # 10 cutting noodles. The udon was boiled for 10 minutes, rinsed with water, subdivided into 100 g glove bags, and stored at 4 캜 for 2 days.
실시예 16의 우동은 2일간 보존한 후에도 양호한 분리성을 유지하고, 탄력 및 찰기를 갖는 양호한 식감이었다.The noodle of Example 16 remained good in separation even after storage for 2 days, and was a good texture with elasticity and stickiness.
Claims (11)
원료 분말 1g당 0.1 내지 5 unit의 아밀라아제를 반죽에 첨가하는 것을 특징으로 하는, 면류의 제조방법.
A lipase having 1 to 20 units of 1,3-position specificity per 1 g of raw material powder, and
Characterized in that 0.1 to 5 units of amylase per 1 g of raw material powder are added to the dough.
원료 분말 1g당 2 내지 15 unit의 1,3-위치 특이성을 갖는 리파아제를 반죽에 첨가하는, 면류의 제조방법.
The method according to claim 1,
A process for producing noodles, comprising adding 2 to 15 units of 1,3-position specificity lipase per gram of raw material powder to the dough.
원료 분말 1g당 0.2 내지 3 unit의 아밀라아제를 반죽에 첨가하는, 면류의 제조방법.
The method according to claim 1,
Wherein 0.2 to 3 units of amylase per 1 g of raw material powder are added to the dough.
원료 분말 1g당 2 내지 15 unit의 상기 1,3-위치 특이성을 갖는 리파아제, 및
원료 분말 1g당 0.2 내지 3 unit의 아밀라아제를 반죽에 첨가하는, 면류의 제조방법.
The method according to claim 1,
2 to 15 units of the above-mentioned 1,3-position specificity lipase per 1 g of raw material powder, and
Wherein 0.2 to 3 units of amylase per 1 g of raw material powder are added to the dough.
아밀라아제는 α-아밀라아제인, 면류의 제조방법.
5. The method according to any one of claims 1 to 4,
Wherein the amylase is? -Amylase.
수용성 헤미 셀룰로오스를 더 포함하는, 면류의 제조방법.
6. The method according to any one of claims 1 to 5,
A method for producing noodles, which further comprises water-soluble hemicellulose.
수용성 헤미 셀룰로오스는 콩류 유래인, 면류의 제조방법.
The method according to claim 6,
Wherein the water-soluble hemicellulose is derived from a legume.
콩류는, 대두 또는 완두콩인, 면류의 제조방법.
8. The method of claim 7,
Wherein the legumes are soybeans or peas.
A noodle obtained by the production method according to any one of claims 1 to 8.
원료 분말 1g당 0.1 내지 5 unit의 아밀라아제를 반죽에 첨가하는 것을 특징으로 하는, 면류의 분리 개량 방법.
A lipase having 1 to 20 units of 1,3-position specificity per 1 g of raw material powder, and
Characterized in that 0.1 to 5 units of amylase per 1 g of raw material powder are added to the dough.
원료 분말 1g당 0.1 내지 5 unit의 아밀라아제를 포함하는 것을 특징으로 하는, 면용 분리 개량제.
A lipase having 1 to 20 units of 1,3-position specificity per 1 g of raw material powder, and
Characterized in that it contains 0.1 to 5 units of amylase per gram of raw material powder.
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JP6905371B2 (en) * | 2017-03-29 | 2021-07-21 | 理研ビタミン株式会社 | Quality improver for instant noodles |
CN108719784A (en) * | 2018-05-08 | 2018-11-02 | 南阳师范学院 | A kind of production method of the nutrition fine dried noodles with weight losing function |
CN109938270A (en) * | 2019-04-03 | 2019-06-28 | 武汉轻工大学 | A kind of malt powder zheganmian and preparation method thereof |
JP7472528B2 (en) * | 2020-02-20 | 2024-04-23 | 味の素株式会社 | Method for producing cooked noodles with improved loosening and texture |
CN112617106B (en) * | 2020-12-18 | 2023-06-20 | 湖州草本连生物科技有限公司 | Selenium-enriched Jin Maimian |
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- 2015-09-25 JP JP2016522833A patent/JP6066016B2/en active Active
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Also Published As
Publication number | Publication date |
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JP6066016B2 (en) | 2017-01-25 |
CN106714573A (en) | 2017-05-24 |
JPWO2016056407A1 (en) | 2017-04-27 |
TW201613480A (en) | 2016-04-16 |
WO2016056407A1 (en) | 2016-04-14 |
TWI656845B (en) | 2019-04-21 |
KR102512181B1 (en) | 2023-03-21 |
CN106714573B (en) | 2020-09-29 |
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