KR20170039870A

KR20170039870A - Cosmetic composition for skin comprising of embryonic stem cell extract of Eleutherococcus seoulensis

Info

Publication number: KR20170039870A
Application number: KR1020150138969A
Authority: KR
Inventors: 이경은; 강상구; 강호덕
Original assignee: 이경은
Priority date: 2015-10-02
Filing date: 2015-10-02
Publication date: 2017-04-12

Abstract

The present invention relates to a skin cosmetic composition containing a stem cell extract of Seoul Ogallippo embryo. The extract of Seoul Ogalli embryo stem cell of the present invention is derived from an endangered plant which is difficult to obtain in a natural state. Seoul Ogbia embryo stem cell extract can be useful as a skin cosmetic composition because it has whitening activity, antioxidative function, and cell stability. The cosmetic composition of the present invention effectively inhibited the enzymatic activity of tyrosinase by blocking the production of melanin and the whitening activity by containing the extract of Seoul Ogallippo embryonic stem cell. In addition, since it is very cytotoxic, it is more safe as a cosmetic composition than arbutin, which is a whitening cosmetic material. Seoul Ogphi Embryonic Stem Cell Extract is a result of the invention which can not be obtained in a natural state and is a useful cosmetic composition for maintaining the health and cleanliness of the skin.

Description

[0001] The present invention relates to a cosmetic composition containing an extract of a stem cell of an oak embryo of Seoul,

The present invention relates to a cosmetic composition comprising an extract from Seoul Ogbia embryo as an active ingredient. The extract of Seoul Ogalli Embryo Stem Cell of the present invention can be obtained only by a technique of inducing embryo of a rare plant Ogalli, which does not exist in a natural state. The active ingredient extracted therefrom has excellent antioxidative and whitening activity, and thus can be usefully used as a cosmetic composition that gives vitality to the skin.

The skin of the human skin is aged due to external environmental nutrients such as outdoor activity, UV exposure, pollutant exposure, and intrinsic natural aging by age. To solve these problems, functional cosmetics containing various substances have been prepared. Is focused on ultraviolet screening and whitening function. Therefore, it is necessary to relieve the tension of the skin nerve and to vitalize the skin through the cell restoration function.

The physiological activity of the skin is imparted with functionalities of cosmetics by adding a substance which imparts improved functionality to the cosmetic rather than general cosmetics. The cosmetic composition for whitening activity uses arbutin as an active ingredient thereof, but if it is contained in a large amount, it may cause side effects. For the purpose of improving wrinkles, there is a cosmetic composition containing adenosine and a skin cell growth factor (EGF).

The photoaging takes place in an inevitable daily life. In everyday life, degeneration of DNA due to UV exposure and reactive oxygen species (ROS) that cause UV are destructive to the skin histologically and clinically. Therefore, various functional antioxidants should be used to prevent collagen and erastin destruction. These antioxidants remove free radicals (FRs) in a variety of ways, so an appropriate combination is needed. Free active ion removal can be accomplished by either 1) neutralizing the free active ions, 2) diluting the peroxide concentration, or restoring the destroyed membrane, 3) inhibiting the formation of ROS by capturing ions, 4) And neutralizing ROS through short fatty acids and cholesterol esters. Therefore, antioxidant substances such as vitamins A, C, B3, niacinamide, vitamin B, adenosine and E are used as functional materials in the production of anti-aging cosmetics. As we have seen, many compounds and materials are currently being utilized in functional cosmetic compositions. However, users tend to demand cosmetic ingredients of natural natural substances such as green tea, for example Epigallocatechin-3-gallate (EGCG), rather than chemicals. In response to this, a composition containing natural and oriental herbal ingredients as raw materials for cosmetics has been developed. Recently, a composition of a cosmetic composition utilizing a delayed aging ability of human stem cells has been developed. However, since the safety of human stem cell extracts is generally unknown, cosmetics based on plant stem cell extracts are being developed.

We will look at the technology of stem cell cosmetics as a technical background. Since stem cells of animals and plants have differentiation and regeneration capabilities, many studies have been conducted in terms of proliferation and reproduction (Tapia et al., 2012; Nakano et al., 2012). In the case of plants, tissue culture technology utilizing the ability to differentiate from somatic embryos through callus proliferation was carried out very long ago. Stem cell technology, which has the ability to self-renew and to differentiate into various types of tissues and organs in undifferentiated cells, has been popularized using the best human stem cells. Stem cell technology is in the early stage of industrialization. In the case of animals, the technology of embryonic stem cells, adult stem cells and induced pluripotent stem cells (iPS) derived from embryos is utilized. In the case of plants, there are stem cells derived from seed mature or callus. When animal stem cells derived from animals including human beings are applied to foods and cosmetics, there is a negative tendency due to ethical problems concerning human dignity and uncertain safety. In this case, it is a problem of animal stem cell cosmetics. Most of cosmetic materials are plant extracts. Therefore, as shown in the case of the present invention, in the case of plant-derived stem cells, the extract sufficiently produces substances necessary for cell differentiation, proliferation, and development for rapid growth as a plant. Therefore, Can be overcome.

Araliaceae plants widely distributed on the Korean Peninsula are Aralia elata), Acanthopanax senticosus , Panax ginseng , Kalopanax pictus , Opulopanax elatus ) and the like. This Aralia genus is a medicinal plant which is widely used as a tonic, including ginseng and augar, and is a medicinal plant which is highly antioxidant, vital, and used as a material for various chronic nitrification treatments, and is used as an edible plant having long-lived function.

However, the present specification discloses that Korean native rare Panax spp., An endangered plant, Eleutherococcus The present invention relates to a plant embryonic stem cell cosmetic composition having a wrinkle-improving function and an antioxidative function, which are produced by mass-producing embryonic stem cells of Seoulensis and extracts thereof. As a reference, it is known that two trees are protected in the National Arboretum of Seoul, Korea ( Eleutherococcus seoulensis ). There are no cosmetic products such as essences, skins, creams and mask packs, which are extracted from the extract of Seoul Ogalli embryonic stem cells.

Accordingly, the present inventors have widely used Araliaceae plants as a material for functional cosmetic compositions, and most of them have been widely used. In particular, there has been a case where a callus inducing embryogenesis of the ogallium in Seoul has been induced (Kang Ho-Deok, Hong-Gyu Kwon, Korea Patent Registration No. 10-0969912). Therefore, the embryonic stem cells of Seoul ogphi ( Eleutherococcus seoulensis ) are not sufficiently harvested from nature, and by improving the conventional technology, inducing and mass-producing the embryonic stem cells of Seoul ogphi ( Eleutherococcus seoulensis ) The present invention is applied to a cosmetic composition for improving wrinkles and whitening, which is excellent in whitening activity and cell stability.

Accordingly, the present invention relates to the use of the rarely-endemic species of the genus Eleutherococcus The present invention also provides a cosmetic composition having skin cell activating function, which comprises an extract of embryonic stem cells of Seoulensis .

In order to solve the above-mentioned problems, the present invention provides a method for propagating stem cells and securing a sufficient material through culturing technology of Seoul ogapi.

In addition, the present invention provides an extract from stem cells of a large scale proliferating S. aureus, and has activity by investigating antioxidant function, tyrosine AIDS inhibition function, melanin formation inhibitory function, cell proliferation degree and the like.

Based on this, the present invention provides a novel cosmetic composition having a wrinkle-improving, whitening, and skin antioxidative function using an extract derived from a stem cell of Seoul olive embryo as an active ingredient.

The cosmetic composition of the present invention is characterized by containing an extract derived from Seoul Ogalli embryo-derived stem cells.

Specifically, the present invention relates to a cosmetic composition for skin improvement comprising an extract derived from a stem cell of Seoul Ogallippo embryo. As described in the present invention, the extract derived from Seoul Ogallippo embryonic stem cells has antioxidative, cell proliferative and inhibitory effects on MMP-1. Therefore, it is effective to inhibit melanin synthesis and inhibit tyrosinase activity, Cosmetics, skin moisturizing and atopic improvement, wrinkle improvement, whitening, prevention of hair loss, hair growth of hair, antibacterial effect of scalp and itching.

Fig. 1 shows that embryos of spherical, heart and torpedo types were formed as various types of embryos including differentiated Seoul augar embryonic stem cells. The present invention uses a substance extracted from Seoul Ogaki embryonic stem cells as a raw material for a new cosmetic composition.
Fig. 2 is a graph showing the results of the measurement of the content of Eleutherococcus a mass-production process through the suspension culture and bioreactor of somatic embryo (germ) of seoulensis).
Fig. 3 is an extract of Seoul ogphalus embryonic stem cell prepared by using water and 70% ethanol as a solvent.
Fig. 4 is a graph showing the antioxidant activity of the extract obtained from Seoul ogphi embryo.
FIG. 5 is a graph showing the results of measuring the degree of inhibition of tyrosinase activity by the extract of S. aureus ES cells.
Fig. 6 is a graph showing the results of testing the melanin synthesis inhibitory ability in the melanocyte B16F10 of the extract of S. aureus ES cells. In contrast to 0.1% arbutin 0.1% in Seoul Ogbia embryonic stem cell extract, melanin synthesis was inhibited to a significant extent.
FIG. 7 is a drawing of cells showing changes in the melanin chromaticity of melanocyte B16F10 treated with the extract of Seoul Ogaki embryo. Cells treated with 0.1% arbutin, 0.1% of the extract from Seoul Ogbia embryo, were similar in blackness.
FIG. 8 is a graph showing the results of testing the effect of the extract of Seoul Ogbpi embryo on cell activity and proliferation. There is no significant inhibition of cell growth and activity even when the stem cell extract of Seoul Ogaki embryo is increased. Dermal keratinocyte HaCaT cells were used.
FIG. 9 shows the results of RT-PCR showing the effect of the extract of Seoul ogphalus embryo on the gene expression of IL-1a and MMP-1 of human fibroblast CCD-986sk. Were used as a comparison group of the same RNA content.
FIG. 10 shows a cosmetic formulation of toner, essence, and cream containing the extract of Seoul Ogphi embryonic stem cells.

Hereinafter, the present invention will be described in detail with reference to examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

In the present invention, the extract derived from Seoul ogpei embryo stem cells is preferably an undifferentiated monocyte containing embryonic stem cells, a globular embryo (spherical embryo), a pear shaped embryo (Heart-shaped embryo) and torpedo breeds, immature belly and young seedlings. The embryonic stem cell-derived extract is a substance that can be easily obtained in a general extraction process.

Preferably, the extract is selected from the group consisting of distilled water, 1,3-butylene glycol, supercritical carbon number, alcohol of 1 to 4, ethyl acetate, and the like. Acetone, and the like. Preferably methanol or ethanol, and most preferably ethanol. After the extraction with the solvent, filtration can be further carried out, and the mixture can be pulverized by concentration under reduced pressure and / or lyophilization. The solvent for extraction may be, but not limited to, 10 to 10000 parts by weight based on 100 parts by weight of the extractant.

The extracts derived from S. aureus ES cells showed antioxidative effects in a concentration dependent manner. It was confirmed that the antioxidative effect was superior through the universal experiments measuring the degree of removal of active oxygen, that is, the DPPH experiment and the ABTS experiment. (See Example 4). The extracts derived from Seoul O. gangue embryo stem cells are particularly effective against B16F10 cells, which are melanocytes (see Example 5). In addition, , The melanin-removing effect showed similar whitening activity as compared with the whitening activity of arbutin (see Example 6). However, since human keratinocyte HaCaT, fibroblast CCD-986sk and melanocyte B16F10 do not have cytotoxicity, they are more safe than arbutin and can be used as a cosmetic composition raw material (see Example 7).

The cosmetic composition of the present invention comprises 0.001 to 20% by weight of the extract derived from Seoul Ogallippo embryonic stem cell, and the content thereof can be controlled by the content of other components contained in the formulation or cosmetic composition. However, when the content of the extract derived from Seoul ogpei embryonic stem cell in the cosmetic composition is less than 0.0001% by weight, it may be difficult to expect a substantial whitening effect in the cosmetic composition. If the content exceeds 20% by weight, none. Preferably, it contains 0.001 to 5.0% by weight of the extract derived from Seoul Ogallippo embryonic stem cell. The cosmetic composition may contain, in addition to the above extract, all kinds of ingredients which can be used in ordinary cosmetics such as excipients, diluents and the like.

The cosmetic composition of the present invention can be provided for use in basic cosmetics, makeup cosmetics, body cosmetics, hair cosmetics, scalp cosmetics, shaving cosmetics, or oral cosmetics. Examples of the basic cosmetics include a cream, a lotion, a pack, a massage cream, and a milky lotion. The makeup cosmetics include a foundation, a makeup base, a lipstick, an eye shadow, an eyeliner, a mascara, an eyebrow pencil, Hair cosmetics, shampoo, rinse, hair treatment, hair mousse, hair liquid, pomade, hair coloring agent, hair blotting agent, coloring agent, Rinse, and scalp cosmetics include hair tonic and scalp treatments. Shaving cosmetics include aftershave lotion and shaving cream. Oral cosmetics include toothpaste and mouth washer. The cosmetic composition of the present invention is not limited to the above, but is preferably used in ointments, essences, lotions, creams, gels, lotions, packs, massage creams, milky lotions, foundations, makeup bases, soaps, liquid cleaners, Sun oil, < / RTI > In order to improve the physical properties of the composition, a perfume, a colorant, a bactericide, an antioxidant, an antiseptic, a moisturizer, a thickening agent, an inorganic salt, a synthetic polymer material and the like may be further added.

For example, when applied as an external ointment for skin, it may be prepared so as to contain 50.0 to 97.0% by weight of petrolatum and 0.1 to 5.0% by weight of polyoxyethylene oleyl-ether phosphate, in addition to the extract derived from Seoul olive embryo-derived stem cells. In addition, when applied as a softening agent, it may be prepared to contain 1.0 to 10.0% by weight of polyhydric alcohols such as propylene glycol, glycerin, and 0.05 to 2.0% by weight of a surfactant such as polyethylene oleyl ether, polyoxyethylene hardened castor oil, and the like . In addition, when applied as a nutritional lotion or a nutritive cream, 5.0 to 20.0% by weight of an oil such as squalene, vaseline, and octyldodecanol and 3.0 to 15.0% by weight of a wax component such as ethanol, stearyl alcohol, . &Lt; / RTI > When it is applied as an essence, it may be prepared so as to contain 5.0 to 30.0% by weight of polyhydric alcohols such as glycerin and propylene glycol. In addition, when applied as a massage cream, it may be prepared so as to contain 30.0 to 70.0% by weight of an oil such as liquid paraffin, petrolatum, isononylisononanoate and the like in addition to the extract derived from Seoul Ogallippo embryonic stem cell, , A fill-off pack containing 5.0 to 20.0% by weight of polyvinyl alcohol or a general emulsified cosmetic may be prepared with a wash-off pack containing 5.0 to 30.0% by weight of pigment such as kaolin, talc, zinc oxide, have. The composition for improving skin of the present invention may be formulated by adding a conventional ingredient to be added to a general cosmetic such as oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a pigment, It is possible to do.

Example 1: Production and mass proliferation of oval germinal embryos of Seoul

In order to obtain stem cell extracts of Seoul Ogphi embryos, the seeds of immature seeds of Seoul Ogphi (Eleutherococcus seoulensis or Acanthopanax seoulense, the same name) were removed and shaking water was added to the sterilized water containing Tween 20 (0.03% After sterilization, surface sterilization was performed in sterile phase. The surface was sterilized by sterilization and sterilized water for 3 minutes in a 70% EtOH solution at 5-minute intervals, sterilized in a solution of 0.5% NaOCl and 0.05% Triton X-100 for 20 minutes, sterilized 5 times for 10 minutes After washing with water, they were transferred to sterilized filter paper to remove water and dentate to solid medium. As a result, the seeds were disinfected according to this method because the contamination degree was very low.

The sterilized seeds were dissected under a microscope and fixed on a solid medium. The callus induction medium was transferred to N6 medium supplemented with 2 mg / L 2,4-D and 1 g / L casein at 30 g / L sucrose and 0.8% agar at 24 ± 2 ℃ incubator to induce callus. For the somatic embryo induction, MS medium was supplemented with 3% sucrose, 0.3% gelite medium, and then with 1,000 mg / L L-glutamine and 1.0 mg / L 2,4- dichlorophenoxyacetic acid. The pH of the solid medium was 6. Eleutherococcus seoulensis grew very well and the induction rate of somatic embryo was also high. The somatic embryos were cultured by treatment with 20 g / L sucrose, 0.3% gelite, 0.10 mg / L ABA in 1 / 2MS medium and 24 hours / day for 16 hours (FIG.

<Example 2> Mass production of oval germinal embryonic stem cells using a cell culture machine

The differentiated embryo plants of Seoul Oagi were mass - produced by suspension culture in the bioreactor of somatic embryo culture media. The size of the embryo was 20 ~ 150um in diameter and 20L scale air - fed incubator was used. 10 ~ 15% of oxygen injection was injected into the culture medium. The large culture period was about 8 ~ 15 days. Seoul Ogalli embryos were produced in a 20 L biological incubator, washed thoroughly, dried and used for extraction (Figure 2).

&Lt; Example 3 >

For example, when 100 g of a sample is taken as an example, dried embryonic stem cells Washed with distilled water, finely cut or crushed, and then extracted with sterilized pure water and 95% ethanol equivalent to 10 times the sample weight. The hot water extraction was carried out at 80 ℃ for 24 hrs. The ethanol extraction was shaked for 24 hours at 25 ° C and 120 rpm. This procedure was repeated three times to obtain an extract. After extraction, it was filtered with filter paper (Advantec No. 2), concentrated using a rotary vacuum concentrator (N-1000, Eyela, Japan) and dried. All materials were stored at 4 ° C until use. In FIG. 3, the extracts of each extract were concentrated with 70% ethanol, dried in a freeze dryer, and powdered. Table 1 shows the extraction yields of 70% ethanol extract and hot water from 100 g of O. gangrene embryonic stem cells.

[Table 1] Items of concrete contents for carrying out the invention

Stem cell extract yield sample Sample weight (g) Extract weight (g) yield(%) Seoul Ogphi Embryonic Stem Cells Ethanol extraction 100 5.2 6.2 Hot water extraction 100 8.1 9.1

< Example 4> Radical scavenging activity of the extracts of oligo- embryonic stem cells from Seoul, Korea

Measurement of DPPH radical scavenging activity

The antioxidant capacity of DPPH radical scavenging activity was measured by modifying the Blois method and it is one of the most commonly used methods for measuring antioxidant activity. DPPH free radical scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical (Sigma, USA) For the experimental method, 250 μl of 0.2 mM DPPH solution dissolved in ethanol was dissolved in 500 μl of each concentration, mixed well with a shaker, allowed to react at room temperature for 30 min, and then subjected to UV / VIS spectrophotometer (Optizen 2120UV, Mecasys, Korea) And the absorbance at 517 nm was measured. As control, ethanol was used instead of DPPH. The activity inhibition rate of DPPH was calculated according to the following equation.

Measurement of ABTS radical scavenging activity

The radical scavenging activity of ABTS [2, 2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] was measured by modifying the Re method. ABTS (Sigma, USA) was dissolved in distilled water at a concentration of 7 mM, and 2.45 mM potassium persulfate was added thereto at a ratio of 1: 1. After reacting in a dark room at room temperature for 24 hours, ABTS radicals were formed and used. The ABTS solution with the radicals was diluted with ethanol and adjusted to an absorbance of 0.70 ± 0.02 at 734 nm. The cleavage efficiency was determined by mixing 400 mL of ABTS solution and 400 mL of sample and incubating for 6 min. After mixing with each concentration sample at 734 nm using UV / VIS Spectrophotometer (Optizen 2120UV, Mecasys, Korea) The percent inhibition of radical activity was calculated according to the following equation.

As a result,

DPPH radical scavenging activity was measured as 1.59% at 10 ㎍ / ㎖, 18.19% at 50 ㎍ / ㎖, 37.79% at 100 ㎍ / ㎖, 57.86% at 500 ㎍ / And 84.10% at 1000 ㎍ / ㎖, respectively.

The ABTS radical scavenging activity was measured at 20.02% at 10 ㎍ / ㎖, 87.35% at 100 ㎍ / ㎖, 98.41% at 100 ㎍ / ㎖, 99.30% at 500 ㎍ / ㎖, 99.50% and 99.70% radical scavenging activity at 1000 占 퐂 / ml (FIG. 4).

& Lt; Example 5 > Results of thyroxine AIDS measurement of embryonic stem cell extracts

Cell Test and Growth Used in the Invention Melanoma cell (B16F10, cell line number: KCLB80008) and human-derived keratinocyte cell (HaCAT) were used as the cells used for the in vivo melanin inhibition test Fibroblast CCD-986sk was purchased from Korea Cell Line Bank (KCLB) and cultured according to general animal cell culture method.

i vitro Tyrosinase inhibition assay

Tyrosinase inhibition assay was performed in vitro to determine the degree of inhibition of tyrosinase activity. In vitro Tyrosinase inhibition assay was performed by modifying the method of Mason et al. For the experiment, 250 μl of 0.1 M phosphate buffer (pH 6.5), 225 μl of diluted sample, 25 μl of mushroom Tyrosinase (2000 U / ml) (Sigma, USA) and 225 μl of 1.5 mM Tyrosine were added in order ℃ for 10 to 15 min. After completion of the reaction, the absorbance is measured at 490 nm using a UV / VIS Spectrophotometer (Optizen 2120UV, Mecasys, Korea). A 0.1M phosphate buffer solution (pH 6.5) was used instead of the sample solution. Arbutin was used as a positive control. The inhibition rate of tyrosinase activity was calculated according to the following equation.

a: Absorbance after reaction of blank

b: Absorbance after reaction of sample liquid

a ', b': absorbance measured by replacing with buffer

In the case of statistical processing,

All experiments were repeated 3 times. Statistical analysis was expressed as mean ± standard deviation. Student's t- test was used to determine statistical significance when the p-value was less than 0.05.

As a result, the extract from Seoul ogbia embryo stem cells significantly inhibited tyrosine AID activity (FIG. 5).

& Lt; Example 6 > To melanoma cells Melanogenesis inhibition test of hot-water extract of oak embryo stem cell in Seoul

B16F10 was cultured in 100 mm dish at 1x10 ^ 6 cells. Twenty-four hours later, the amount of the extract was determined for 72 hours in DMEM + FBS + antibiotics. B16F10 was collected, 1 ml of 10% DMSO in NaOH was added, and incubated in a constant temperature water bath at 37 ° C for 24 hours. Absorbance was recorded at 490 nm using a spectrophotometer to measure the degree of melanin inhibition. The inhibition rate of melanin formation was measured by the same method as above using Arbutin as a positive control. The degree of inhibition was measured using the following formula.

Melanogenesis (%) = [(A-B) / A] X 100

A: control (amount of melanin in cells without added sample)

B: Amount of melanin in cells to which sample is added

Increased concentrations of 0.001%, 0.01%, and 0.1% of water extracts from Seoul ogpei embryonic stem cells in B16F10 cells inhibited the production of melanin up to 56%. This was 43.4% when treated with 0.1% albutin, and there was no significant difference (Table 2). That is, the production of melanin was markedly suppressed. The degree of decolorization of melanin was similar to that of 0.1% of albutin. This indicates that the hot water extract of Seoul Ogalli embryo stem cell has an excellent whitening effect (FIGS. 4 and 5)

[Table 2] Items of concrete contents for carrying out the invention

Melanin production of hot-water extracts from olive embryos of Seoul density(%) 0 0.001 0.01 0.1 0.1 Melanin production rate (%) 100 ± 0.2 94.4 ± 2.1 82.8 ± 1.5 56 ± 3.0 43.4 ± 2.1

< Example 7> Cytotoxicity test and proliferation test of hot water extract of oligo- embryonic stem cell of Seoul

Cultures of HaCaT cells and CCD-986sk were cultured in DMEM (Dulbecco's modified Eagle's medium, Sigma) containing 10% FBS and 2% penicillin / streptomycin in a cell incubator maintained at 37 ° C and 5% The culture medium was exchanged every 2 to 3 days and subcultured. The effect of stem cell extracts from ogapi embryos on cytotoxicity and proliferation of HaCAT cells, a keratinocyte derived from human skin, was investigated. Specifically, the cells were cultured for 48 or 72 hours with various concentrations of the extracts of Seoul Ogalli embryo, followed by MTT assay and cell viability. The control group was supplemented with 0.1% DMSO only in the medium without the extract. As a result, cell survival rate was about 86% even at 0.1% increase in the hot water extract concentration of Seoul Ogbp embryo stem cell. In 0.05% cell survival rate was 95% (hot water extract) and 100% (ethanol extract). As a conclusion, the extracts of Ogakpi embryonic stem cells from Seoul showed no serious toxicity to the cells (Fig. 8).

< Example 8> Inhibition of gene expression of MMP- 1 and IL- 1α in hot water extract of olive embryo- derived stem cells from Seoul

Dermal fibroblast CCD-986sk was applied to a 100-mm dish at a density of 1x10 6 cells. Cells were treated with 0.001%, 0.01%, and 0.1% of hot water extracts of O. ganglionium embryos and cultured for 72 hours. After the cells were harvested, total RNA was extracted and cDNA was synthesized using the same amount of RNA. Expression of GAPDH, IL-1α, and MMP-1 was examined by RT-PCR using the synthesized cDNA. Table 2 shows the primers of each gene used. As a result, the expression level of IL-1a and MMP-1 was significantly lowered as the concentration increased. The expression of MMP-1 in the absence of the extract was 61% on average, but was significantly reduced to about 14% of the cells treated with 0.1% of the extract. In the case of IL-1a, the expression level of the gene was 66% when the extract was not treated, but significantly decreased to about 8% of the treated cells (FIG. 9). MMP-1 is involved in collagen degradation and is a UV-induced monolayer enzyme that nourishes skin aging. In addition, IL-1a is an indicator protein that is involved mainly when inflammation occurs. Therefore, the extract of Ogakpi embryos from Seoul can suppress the progression of aging of cells by inhibiting MMP-1 vesicle, and the low expression of IL-1a may inhibit inflammation of skin. Therefore, the extract of Ogaki embryo stem cell of Seoul can be used as a composition of anti-aging cosmetic composition and a composition of atopic skin cosmetic composition.

[Table 3] Items of specific contents for carrying out the invention

Primers sequences of genes used in the present invention for gene expression investigation Primer Direction 5'-oligonucleotide sequence-3 ' IL-1? forward CAT GTC AAA TTT CAC TGC TTC ATC C reverse GTC TCT GAA TCA GAA ATC CTT CTA TC MMP-1 forward AGC GTG TGA CAG TAA GCT AA reverse GTT TTC CTC AGA AAG AGC AGCAT GAPDH forward CGG AGT CAG CGG ATT TGG TCG TAT reverse AGC CTT CTC CAT GGT GGT GAA GAC

[Table 4] Items of concrete contents for carrying out the invention

Expression of IL-1α and MMP-1 in RT-PCR after Treatment of Seoul Ogbai Embryonic Stem Cell Extract with Dermal Fibroblast CCD-986sk density(%) 0 0.001 0.01 0.1 IL-1? 66.22 + 1.36 68.53 + - 2.88 45.36 ± 1.05 8.84 ± 1.80 MMP-1 61.13 + - 0.84 55.49 + - 0.37 45.87 + - 0.35 14.4 ± 0.20 GAPDH 57.88 + 1.89 61.96 + 1.34 58.49 + - 1.13 60.41 + - 2.62

&Lt; Preparation Example > Preparation of cosmetic composition

A composition of a cosmetic composition was prepared using the hot water extract of Seoul Ogalli embryo stem cell of the present invention. The composition of the present invention was prepared by formulating a general composition cream, a whitening cream, a moisturizing cream, an ultraviolet screening cream, an essence, a toner, and a body lotion composition and controlling the concentration of the extract of Seoul Ogbia embryo.

<Preparation Example 1> Formulation of cream made from Seoul O-gang embryo stem cell extract as a raw material

[Table 5] Items of concrete contents for carrying out the invention

Cream containing the extract of Ogaki embryo stem cells from Seoul NO INCI Name Ingredients Contents
(%) CAS No. Reference Function One Water Purified water 7732-18-5 ICID SOLVENT 2 Caprylic / Capric Triglyceride Caprylic / capric triglyceride 65381-09-1
73398-61-5 ICID SKIN-CONDITIONING AGENT 2 Butylene Glycol Butylene glycol 107-88-0 ICID SKIN-CONDITIONING AGENT 3 Squalane Squalane 111-01-3 ICID SKIN-CONDITIONING AGENT 8 Glyceryl Stearate Glyceryl monostearate 123-94-4
11099-07-3
31566-31-1
85666-92-8 ICID SKIN-CONDITIONING AGENT 5 Sodium Hyaluronate Sodium hyaluronate 9067-32-7 ICID SKIN-CONDITIONING AGENT Niacinamide Niacinamide 98-92-0 ICID SKIN-CONDITIONING AGENT 6 Coceth-7 Courtesy-7 - ICID EMULSIFYING AGENT 7 PPG-1-PPG9 Lauryl Glycol Ether Piperazine-9-laurylglycol ether - ICID EMULSIFYING AGENT 8 PEG-40 Hydrogenated Castor Oil PIGI-40 Hydrogenetite Castor Oil 61788-85-0
(Generic) ICID EMULSIFYING AGENT 9 Polyacrylamide Polyacrylamide 9003-05-08 ICID VISCOSITY INCREASING AGENTS 10 Phenoxyethanol Phenoxyethanol 122-99-6 ICID PRESERVATIVE 11 Ammonium Acryloyldimethyltaurate / VP Copolymer Ammonium acryloyldimethyltaurate / Vpicopolymer - ICID EMULSIFYING AGENT 12 Citric Acid Citric acid 77-92-9
5949-29-1 ICID pH ADJISTERS 13 Sophora Angustifolia Root Extract Gosam extract - ICID SKIN-CONDITIONING AGENT 14 MeliaAzadirachtaExtract Indian mulberry tree extract 90063-92-6 ICID SKIN-CONDITIONING AGENT 15 CitrusParadisi (Grapefruit) FruitExtract Grapefruit extract - ICID SKIN-CONDITIONING AGENT 16 Acetyl Hexapetide-3 (Argireline) Acetyl hexapeptide-3 (azirreline) - ICID SKIN-CONDITIONING AGENT 17 Adenine Adenosine 58-61-7 ICID SKIN-CONDITIONING AGENT 18 Disodium EDTA Disodium iodide 139-33-3 ICID CHELATING AGENTS 19 Aloe Barbadensis Leaf Extract Aloe vera leaf extract 85507-69-3
94349-62-9 ICID SKIN-CONDITIONING AGENT 20 Lavabdula Angustifolia (LavenderP Flower Extract Lavender flower extract - ICID SKIN-CONDITIONING AGENT 21 Chamomilla Recutita (Matricaria) Flower Extract Chamomile flower extract 84649-86-5
84082-60-0 ICID SKIN-CONDITIONING AGENT 22 Centella Asiatica Extract Centipede extract 84696-21-9
84776-24-9 ICID SKIN-CONDITIONING AGENT 23 Camellia Sinensis Leaf Extract Green tea extract 84650-60-2 ICID SKIN-CONDITIONING AGENT 24 Zanthoxylum Piperitum Fruit Extract Pine nut fruit extract 97404-53-0 ICID SKIN-CONDITIONING AGENT 25 Pulsatilla Koreana Extract Waxy extract - ICID SKIN-CONDITIONING AGENT 26 Usnea Barbata (Lichen) Extract Earthier extract 84696-53-7 ICID SKIN-CONDITIONING AGENT 27 Eleutherococcus Seoulensis embryonic cell extract Seoul Ogphi Embryonic Stem Cells extract 0.001 to 5 2-04-2015-3528 ICID ANTI-OXIDANT 28 Parfum Spices - ICID FRAGRANCE Sum ~ 100 -Total 27 items - All ingredients are listed in ICID

To prepare the cream, as shown in Table 5, the phase was prepared by separating according to the properties of the material during stirring, and the mixture was stirred at a suitable temperature while stirring. The above composition was finally stirred while being cooled to 35 to 40 ° C.

< Manufacturing Example 2> Seoul Ogphy Embryonic Stem Cell Extract Production of Toner Formulation Using Raw Material

[Table 6] Items of concrete contents for carrying out the invention

A toner containing the extract of Ogaki embryo cell of Seoul NO INCI Name Ingredients Contents
(%) CAS No. Reference Function One Water Purified water 7732-18-5 ICID SOLVENT 2 Dipropylene Glycol Dipropylene glycol 110-98-5
25265-71-8 ICID SKIN-CONDITIONING AGENT 3 Alcohol ethanol 64-17-5 ICID SOLVENT 4 Butylene Glycol Butylene glycol 107-88-0 ICID SKIN-CONDITIONING AGENT 5 Panthenol Panthenol 81-13-0
(D-Form)
16485-10-2 ICID SKIN-CONDITIONING AGENT 6 Betaine Betaine 107-43-7 ICID SKIN-CONDITIONING AGENT 7 Glucerin glycerin 56-81-5 ICID SKIN-CONDITIONING AGENT 8 Niacinamide Niacinamide 98-92-0 ICID SKIN-CONDITIONING AGENT 9 SodiumCitrate Sodium citrate 68-04-2
6132-04-3 ICID pH ADJISTERS 10 Phenoxyethanol Phenoxyethanol 122-99-6 ICID PRESERVATIVE 11 PEG-60 Hydrogenated Castor Oil PIGGY-60 Hydrogenated Castor Oil 61788-85-0 ICID EMULSIFYING AGENT 12 Citric Acid Citric acid 77-92-9
5949-29-1 ICID pH ADJISTERS 13 Sophora Angustifolia Root Extract Gosam extract - ICID SKIN-CONDITIONING AGENT 14 MeliaAzadirachtaExtract Indian mulberry tree extract 90063-92-6 ICID SKIN-CONDITIONING AGENT 15 CitrusParadisi (Grapefruit) FruitExtract Grapefruit extract - ICID SKIN-CONDITIONING AGENT 16 Trehalose Trehalose 99-20-7 ICID Humectant 17 Adenine Adenosine 58-61-7 ICID SKIN-CONDITIONING AGENT 18 Disodium EDTA Disodium iodide 139-33-3 ICID CHELATING AGENTS 19 Aloe Barbadensis Leaf Extract Aloe vera leaf extract 85507-69-3
94349-62-9 ICID SKIN-CONDITIONING AGENT 20 Lavabdula Angustifolia (LavenderP Flower Extract Lavender flower extract - ICID SKIN-CONDITIONING AGENT 21 Chamomilla Recutita (Matricaria) Flower Extract Chamomile flower extract 84649-86-5
84082-60-0 ICID SKIN-CONDITIONING AGENT 22 Centella Asiatica Extract Centipede extract 84696-21-9
84776-24-9 ICID SKIN-CONDITIONING AGENT 23 Camellia Sinensis Leaf Extract Green tea extract 84650-60-2 ICID SKIN-CONDITIONING AGENT 24 Zanthoxylum Piperitum Fruit Extract Pine nut fruit extract 97404-53-0 ICID SKIN-CONDITIONING AGENT 25 Pulsatilla Koreana Extract Waxy extract - ICID SKIN-CONDITIONING AGENT 26 Usnea Barbata (Lichen) Extract Earthier extract 84696-53-7 ICID SKIN-CONDITIONING AGENT 27 Eleutherococcus Seoulensis embryonic cell extract Seoul Ogphi Embryonic Stem Cells extract 0.001 to 5 2-04-2015-3528 ICID ANTI-OXIDANT 28 Parfum Spices - ICID FRAGRANCE Sum 100.0100 -Total 27 items - All ingredients are listed in ICID

As shown in Table 6, the toner was prepared by separating the phases according to the properties of the toner during stirring, and the toner was mixed with stirring at a proper temperature. The above composition was finally stirred while being cooled to 35 to 40 ° C.

< Manufacturing Example 3> Seoul Ogphy Embryonic Stem Cell Extract Manufacture of essence formulations made from raw materials

[Table 7] Items of concrete contents for carrying out the invention

Essence containing the extract of Ogaki embryo stem cell in Seoul NO INCI Name Ingredients Contents
(%) CAS No. Reference Function One Water Purified water 7732-18-5 ICID SOLVENT 2 Caprylic / Capric Triglyceride Caprylic / capric triglyceride 65381-09-1
73398-61-5 ICID SKIN-CONDITIONING AGENT 2 Butylene Glycol Butylene glycol 107-88-0 ICID SKIN-CONDITIONING AGENT 3 Squalane Squalane 111-01-3 ICID SKIN-CONDITIONING AGENT 8 Glyceryl Stearate Glyceryl monostearate 123-94-4
11099-07-3
31566-31-1
85666-92-8 ICID SKIN-CONDITIONING AGENT 5 Sodium Hyaluronate Sodium hyaluronate 9067-32-7 ICID SKIN-CONDITIONING AGENT Niacinamide Niacinamide 98-92-0 ICID SKIN-CONDITIONING AGENT 6 Coceth-7 Courtesy-7 - ICID EMULSIFYING AGENT 7 PPG-1-PPG9 Lauryl Glycol Ether Piperazine-9-laurylglycol ether - ICID EMULSIFYING AGENT 8 PEG-40 Hydrogenated Castor Oil PIGI-40 Hydrogenetite Castor Oil 61788-85-0
(Generic) ICID EMULSIFYING AGENT 9 Polyacrylamide Polyacrylamide 9003-05-08 ICID VISCOSITY INCREASING AGENTS 10 Phenoxyethanol Phenoxyethanol 122-99-6 ICID PRESERVATIVE 11 Ammonium Acryloyldimethyltaurate / VP Copolymer Ammonium acryloyldimethyltaurate / Vpicopolymer - ICID EMULSIFYING AGENT 12 Citric Acid Citric acid 77-92-9
5949-29-1 ICID pH ADJISTERS 13 Sophora Angustifolia Root Extract Gosam extract - ICID SKIN-CONDITIONING AGENT 14 MeliaAzadirachtaExtract Indian mulberry tree extract 90063-92-6 ICID SKIN-CONDITIONING AGENT 15 CitrusParadisi (Grapefruit) FruitExtract Grapefruit extract - ICID SKIN-CONDITIONING AGENT 16 Acetyl Hexapetide-3 (Argireline) Acetyl hexapeptide-3 (azirreline) - ICID SKIN-CONDITIONING AGENT 17 Adenine Adenosine 58-61-7 ICID SKIN-CONDITIONING AGENT 18 Disodium EDTA Disodium iodide 139-33-3 ICID CHELATING AGENTS 19 Aloe Barbadensis Leaf Extract Aloe vera leaf extract 85507-69-3
94349-62-9 ICID SKIN-CONDITIONING AGENT 20 Lavabdula Angustifolia (LavenderP Flower Extract Lavender flower extract - ICID SKIN-CONDITIONING AGENT 21 Chamomilla Recutita (Matricaria) Flower Extract Chamomile flower extract 84649-86-5
84082-60-0 ICID SKIN-CONDITIONING AGENT 22 Centella Asiatica Extract Centipede extract 84696-21-9
84776-24-9 ICID SKIN-CONDITIONING AGENT 23 Camellia Sinensis Leaf Extract Green tea extract 84650-60-2 ICID SKIN-CONDITIONING AGENT 24 Zanthoxylum Piperitum Fruit Extract Pine nut fruit extract 97404-53-0 ICID SKIN-CONDITIONING AGENT 25 Pulsatilla Koreana Extract Waxy extract - ICID SKIN-CONDITIONING AGENT 26 Usnea Barbata (Lichen) Extract Earthier extract 84696-53-7 ICID SKIN-CONDITIONING AGENT 27 Eleutherococcus Seoulensis embryonic cell extract Seoul Ogphi Embryonic Stem Cells extract 0.001 to 5 2-04-2015-3528 ICID ANTI-OXIDANT 28 Parfum Spices - ICID FRAGRANCE Sum 104.6300 All ingredients are listed in ICID

To prepare the essence, phases were prepared according to the properties of physical properties during stirring as shown in Table 6, and mixed at a suitable temperature while stirring slowly. The above composition was finally stirred while being cooled to 35 to 40 ° C.

< Manufacturing Example 4> Manufacture of body lotion formulation made from the extract of Ogaki embryo cell from Seoul

[Table 8] Items of concrete contents for carrying out the invention

Body lotion containing extract from Seoul Ogbia embryo stem cells NO INCI Name Ingredients Contents
(%) CAS No. Reference Function One Water Purified water 7732-18-5 ICID SOLVENT 2 Caprylic / Capric Triglyceride Caprylic / capric triglyceride 65381-09-1
73398-61-5 ICID SKIN-CONDITIONING AGENT 3 Glycerin glycerin 56-81-5 ICID SKIN-CONDITIONING AGENT 4 Cyclopentasiloxane Cyclopentasiloxane 541-02-6 ICID HAIR CONDITIONING AGENTS 5 Cyclohexasiloxane Cyclohexasiloxane 540-97-6 ICID HAIR CONDITIONING AGENTS 6 Polysorbate 60 Polysorbate 60 9005-67-8 (Generic) ICID EMULSIFYING AGENT 7 Cetearyl Alcohol Cetearyl alcohol 8005-44-5
67762-27-0 ICID VISCOSITY INCREASING AGENTS 8 Glyceryl Stearate Glyceryl monostearate 123-94-4
11099-07-3
31566-31-1
85666-92-8 ICID SKIN-CONDITIONING AGENT 9 Glyceryl Stearate Glyceryl stearate 123-94-4
11099-07-3
31566-31-1
85666-92-8 ICID SKIN-CONDITIONING AGENT 10 PEG-100 Stearate FAGE -100 stearate 9004-99-3 ICID SURFACTANT 11 Stearic Acid Stearic acid 1957-11-04 ICID EMULSIFYING AGENT 12 Biosaccharide Gum-1 Bai Osaka Ride Gum -1 - ICID SKIN-CONDITIONING AGENT 13 Moringa Oleifera Leaf Extract Drumstick leaf extract 93165-54-9 ICID SKIN-CONDITIONING AGENT 14 Butylene Glycol Butylene glycol 107-88-0 ICID SKIN-CONDITIONING AGENT 15 1,2-Hexanediol 1,2-hexanediol 6920-22-5 ICID SOLVENT 16 Caprylyl Glycol Caprylic glycol 1117-86-8 ICID SKIN-CONDITIONING AGENT 17 Tropolone Troponone - ICID CHELATING AGENTS 18 Sorbitan Sesquioleate Sorbitan sesquioleate 8007-43-0 ICID EMULSIFYING AGENT 19 Dimethicone Dimethicone 9006-65-9
9016-00-6
63148-62-9 ICID SKIN-CONDITIONING AGENT 20 Butyrospermum Parkii (Shea) Butter Sheer butter 194043-92-0 ICID SKIN-CONDITIONING AGENT 21 Phenoxyethanol Phenoxyethanol 122-99-6 ICID PRESERVATIVE 22 Carbomer Carbomer 9003-01-4
9007-16-3
9007-17-4
9062-04-8
76050-42-5 ICID VISCOSITY INCREASING AGENTS 23 Arginine Arginine 74-79-3
(L-Form)
7200-25-1 SKIN-CONDITIONING AGENT 13 VanillylButylEther Vanillyl butyl ether 82654-98-6 ICID SKIN-CONDITIONING AGENT 25 Trapa Japonica Fruit Extract Fruit extract - ICID SKIN-CONDITIONING AGENT 26 Sophora Angustifolia Root Extract Gosam extract - ICID SKIN-CONDITIONING AGENT 27 MeliaAzadirachtaExtract Indian mulberry tree extract 90063-92-6 ICID SKIN-CONDITIONING AGENT 28 CitrusParadisi (Grapefruit) FruitExtract Grapefruit extract - ICID SKIN-CONDITIONING AGENT 29 Eleutherococcus Seoulensis embryonic cell extract Seoul Ogphi Embryonic Stem Cells extract 0.001 to 5 2-04-2015-3528 ICID ANTI-OXIDANT 30 Allantoin Allantoin 97-59-6 ICID SKIN-CONDITIONING AGENT 31 Disodium EDTA Disodium iodide 139-33-3 ICID CHELATING AGENTS 32 Parfum Spices - ICID FRAGRANCE Sum 100.0100

To prepare the body lotion, as shown in Table 7, the phase was prepared by separating according to the properties of the physical properties upon stirring, and the mixture was stirred at a proper temperature while stirring. The above composition was stirred while being cooled to 35 to 40 占 폚.

<Experimental Example 1>

The appearance safety of the cosmetic composition of the present invention

To study the safety of developing cosmetics using the raw materials developed in the present invention, the test was conducted according to the Guideline for Safety Guideline of the Agency.

A part of the result obtained by preparing a cosmetic composition by adding a raw material to an extract of Seoul Ogbia embryo was used as an example (the temperature stability test of the base composition was performed by changing the contents of the cosmetic product according to the temperature conditions Observation items (appearance and change of incense): color difference, fading, streaks, color stain, foreign matter contamination, wounds, floatation, separation, sedimentation, sweating, excretion The measurement of pH, hardness, viscosity, turbidity, emulsion, etc. were made by measuring properties such as crack, gelation, transparency, caking, air bubbles, pinhole, floating, fungus, soaring, And the particle diameter of the powder particles, the amount of drying loss of water, the type of emulsification, the softening point, and the melting point.

Stability test result by temperature

As shown in Table 9 of the developed cosmetics using the extract of Seoul O-gang embryo stem cell developed in the present invention, the safety by temperature was very good (Table 9).

[Table 9] Items of specific contents for carrying out the invention

Stability test result by temperature extract
density 0% 0.01% 0.1% 1 ~
30 days Degree of change detach Sedimentation Fling discoloration detach Sedimentation Fling discoloration detach Sedimentation Fling discoloration 5 ℃ toner normal normal normal normal normal normal normal normal normal normal normal normal essence normal normal normal normal normal normal normal normal normal normal normal normal cream normal normal normal normal normal normal normal normal normal normal normal normal 25 ℃ toner normal normal normal normal normal normal normal normal normal normal normal normal essence normal normal normal normal normal normal normal normal normal normal normal normal cream normal normal normal normal normal normal normal normal normal normal normal normal 40 ℃ toner normal normal normal normal normal normal normal normal normal normal normal normal essence normal normal normal normal normal normal normal normal normal normal normal normal cream normal normal normal normal normal normal normal normal normal normal normal normal

As a result of the temperature cycling test of the cosmetics using the extract of Seoul O-gang embryo stem cell developed in the present invention, the state change was observed after storage for 24 hours at each temperature. As a result, no abnormality was found as shown in Table 10.

[Table 10] Items of concrete contents for carrying out the invention

Temperature cycling test result toner essence cream Extract concentration

Temperature (℃)
0%
0.01%
0.1%
0%
0.01%
0.1%
0%
0.01%
0.1%
-10
No change
Frozen state
Frozen state
It is a little hardened, and does not change, discoloration, separation, precipitation.
It is a little hardened, and does not change, discoloration, separation, precipitation. 0 clear clear clear 10 clear clear clear 25 clear clear clear 40 clear clear clear

<Experimental Example 2>

The safety investigation according to the pH change of the cosmetic composition of the present invention

The pH stability of the composition was investigated for 30 days after manufacturing the cosmetic formulation made from the extract of Seoul Oki embryo stem cell which was developed in the present invention. As shown in Table 11, there was no change in pH.

[Table 11] Items of concrete contents for carrying out the invention

The results of the pH change of the cosmetic formulations containing the extract of Ogaki embryo stem cell (25 ℃) toner essence cream Extract concentration 0% 0.01% 0.1% One% 0% 0.01% 0.1% One% 0% 0.01% 0.1% 1 day 5.53 5.50 5.36 4.59 5.91 5.88 5.78 4.90 5.96 5.79 5.19 3 days 5.52 5.40 5.37 4.60 5.92 5.89 5.80 5.00 5.96 5.81 5.20 5 days 5.52 5.50 5.36 4.59 5.91 5.88 5.78 4.90 6.00 5.79 5.19 7 days 5.53 5.40 5.36 4.59 5.91 5.89 5.78 4.90 5.96 5.79 5.19 15th 5.54 5.50 5.37 4.60 5.92 5.89 5.79 5.00 5.96 5.80 5.21 30 days 5.53 5.50 5.36 4.59 5.91 5.88 5.78 4.90 5.98 5.79 5.19 Average 5.53 2.76 2.68 2.30 5.91 2.95 2.89 2.46 5.96 2.90 2.60 Standard Deviation 0.008 3.882 3.789 3.239 0.005 4.151 4.086 3.458 0.017 4.087 3.669

<1-1> Stability of Toner

As shown in Tables 9, 10, and 11, the stability of the toner prepared with the extract from Seoul Ogbia embryo stem cell over time for 30 days showed no change or any specific physical change.

<1-2> Essence stability

As shown in Tables 9, 10, and 11, the essence prepared from the extract of Seoul Ogalli embryo stem cell was examined for safety over time for 30 days, and no denaturation or specific physical change was observed.

<1-3> Stability of Cream

As shown in Tables 9, 10, and 11, the safety of the cream prepared with the extract from Seoul Ogbia embryo stem cell after 30 days was not affected by degeneration or specific physical changes.

Claims

Seoul Ogphi Embryonic Stem Cell Extract ( Eleutherococcus Seoulensis embryonic extract. At that time, the scientific name of Seoul Ogalli was Eleutherococcus seoulensis Or the same scientific name Acanthopanax seoulensis .

The cosmetic composition according to claim 1, wherein the composition comprises the extract from Seoul Ogbia embryo.

2. The cosmetic composition according to claim 1, wherein the stem cells are extracted with at least one solvent selected from the group consisting of distilled water, 1,3-butylene glycol, alcohol having 1 to 4 carbon atoms, ethyl acetate and acetone .

The cosmetic composition according to claim 1, wherein the cosmetic composition is at least one selected from the group consisting of ointment, essence, lotion, cream, gel, lotion, pack, massage cream, milky lotion, foundation, makeup base, soap, liquid detergent, &Lt; / RTI > wherein the composition has a formulation selected from the group consisting of

The cosmetic composition according to claim 1, wherein the cosmetic composition contains 0.001 to 20% by weight of an extract of Seoul Ogallippo embryonic stem cell relative to the whole composition.