KR20170029459A - Anchovy calcium products of senior-friendly using oriental medicinal herb - Google Patents

Abstract

The present invention relates to a senior-friendly and high-quality anchovy calcium product using medicinal herbs, capable of preventing calcium-related diseases (osteoporosis, atherosclerosis, heart diseases, colorectal cancer, and others), and minimizing side effects from calcium intake through the intake of the anchovy calcium product mixed with eco-friendly medicinal herbs. The present invention can provide senior-friendly functional food having improved functionality through the mixing with the anchovy calcium as an excellent calcium supply source, by comprising anchovy calcium powder and a medicinal herb extract of any one of Rehmannia glutinosa, Angelica gigas Nakai, Drynariae Rhizoma, Lycium chinense, Eucommia ulmoides, Achyranthes japonica, Japanese cinnamon, Phlomis Radix, Glycyrrhiza uralensis, and Cibotium barometz, or a medicinal herb extract of a mixture thereof.

Description

Anchovy calcium products of senior-friendly using oriental medicinal herb

The present invention relates to an age-friendly, high-quality anchovy calcium agent using a herbal material, and more particularly to an ingestion of anchovy calcium supplemented with an environmentally friendly herbal ingredient for diseases (osteoporosis, atherosclerosis, heart disease, colon cancer, etc.) To an aging-friendly high quality anchovy calcium agent using a herbal ingredient which prevents calcium related diseases and minimizes side effects on calcium intake.

Calcium is involved in the growth and maintenance of bones and teeth, blood clotting, and muscle contraction and relaxation. Inadequate intake of calcium leads to changes in skeletal and dental tissues, decreases growth, and causes bone malformations.

According to the results of the nutrition survey of Koreans, calcium intake is still below the recommended amount. According to the Korean Nutrition Survey (2010, Korean Nutrition Society), 700 mg (over 50 years old) for adults and 750 mg (for women aged 49 to 69 years) (Over the age of 50). In adolescents (15-18 years), men and women are required to consume 900 mg and 800 mg more than adults, but actual calcium intake is present in almost all age groups. In particular, osteoporosis, which is closely related to calcium deficiency, is becoming a serious problem for middle-aged women. The risk factors for osteoporosis are decreased body weight / height, white or Asian, family history, decreased calcium intake, lack of exercise, nulliparity, alcoholism, smoking, caffeine overdose, premature menopause, amenorrhea, Fracture history, thyroid gland, disease, cancer, liver, large intestine, kidney disease, etc.

Calcium is the most abundant minerals in the body and usually contains about 1, 500 to 1,500 g of body weight, 1.52%. About 99% of the body calcium forms the skeleton and teeth, and only about 1% of the body regulates physiological activity such as contraction and relaxation of muscle, coagulation of regular heartbeat blood, enzyme activity, delivery of intracellular stimulation and excitement (Allen and Wood, 1994).

As such, calcium having a variety of physiological activity control functions should be maintained at a constant concentration in the body. If a long-term intake of calcium is insufficient, osteoporosis, rickets, and deficiency such as tetany may be induced, Its importance is emphasized.

Recently, calcium is closely related to the growth period, post-adulthood skeletal disease, circulatory system disease, and bowel disease. However, because of the fact that sufficient calcium is not obtained only by the normal diet, the demand for calcium-containing foods is increasing. And calcium-containing foods are commercially available. However, the most widely used bones are banned from importing due to safety problems caused by mad cow disease, and it is urgent to develop new calcium materials.

In addition, osteoporosis, atherosclerosis, heart disease, and colon cancer, which frequently occur in aged people in the aging society, are deeply related to calcium, and in women, menopause causes secretion of estrogen, a female hormone, Because of the decrease in body absorption, women's osteoporosis rates are about four times higher than that of men, accounting for 13% of the total population. The dietary habits of increased fat and phosphate intake compared to the past, Studies have shown that intake of calcium is inhibited.

Recently, as interest in health has increased, various kinds of health supplements have been used, and consumption of calcium supplements has also been increasing, and various calcium supplements have been imported and marketed. However, the calcium component has a very low solubility, so there are many restrictions on the production of the product. Abuse and misuse of the calcium supplements may cause side effects such as absorption of other minerals, gastrointestinal disorders, constipation, and sickling.

One of the major sources of calcium, anchovies ( Engraulis japonicus ) is a multipurpose, small, red fleshy fish having a body length of 1 to 15 cm, distributed evenly throughout the world, but is a major offshore fish resource produced throughout the year on the Korean coast. The temperature of the anchovy is in the range of 13 ~ 23 ℃, and it is distributed in all coastal areas of East Sea, West Sea and Namhae in Korea. However, the catch is high in Busan, Gyeongnam and Chonnam regions. Seasonally, It is being harvested as a source of income for major offshore fishermen by being caught in the Kwonhyeon network,

In Korea, annual catch of anchovy is about 20 ~ 30 thousand tons per year, which is very large as a single species. However, since the raw fish is easily decomposed, it has to be processed into a product that is storable immediately after harvest. The existing anchovy processing methods are consumed in the form of anchovy products with economical efficiency of only 30 ~ 40% of the total catches, and the anchovy catches of 60 ~ 70% level are suitable for temporary mass processing, Of salted fish products.

On the other hand, anchovy fish generally contain a large amount of calcium, so it tends to be recognized only as a typical high calcium food material. In addition to calcium, it contains a high-quality protein, a relatively large amount of fat including EPA and DHA, And minerals and nucleic acids, as well as nutritive and palatable characteristics of the raw materials of processed foods, which have a very high potential value for use.

However, since the decay rate is faster than other fish species, most of the catches are used as raw materials for fermented seafood and fish sauce (Fig. It is necessary to develop a calcium supplement to maximize calcium nutrition in growing period and popularity by utilizing the byproducts which are made from anchovy sauce and are currently being used as feed or the like through various disposal processes.

One example of such anchovy and herbaceous techniques is described in documents 1 and 2 below.

For example, as shown in FIG. 1, an anchovy is pulverized into a powder having a size of 30 to 60 mesh, and anchovy powder is placed in a 2 to 10 times weight ratio Drying the dried anchovy powder at 40 to 50 ° C for 1 to 3 hours, filtering the dried anchovy powder with citric acid, malic acid, lactic acid, Acetic acid in an amount of 0.5 to 1.5% of organic acid, immersing the mixture at 20 to 50 DEG C for 30 minutes to 2 hours, and then drying at 40 to 50 DEG C for 1 to 3 hours. Lt; / RTI >

In addition, Patent Document 2 discloses that an anchovy preparation for eliminating fishy smell is applied to anchovy processing, and a functional anchovy processing solution is prepared by hot water extraction of the herbal medicine materials according to the prescription of the high grade excipients of the fragments, , Dried and dried anchovy naturally, dried in an green tea extract to remove the intestine and increase the flavor of naturally dried anchovy, dried to less than 10% of moisture for commercialization, A functional anchovy according to a herbal medicine prescription which can prevent the fever (tidal fever), self-sweating, diarrhea, seawater and dandruff caused by the lack of gye blood is disclosed and a processing method thereof .

Korean Registered Patent No. 10-0705790 (Registered on Apr. 03, 2007) Korean Registered Patent No. 10-0655148 (Registered on December 01, 2006)

However, in the conventional techniques as described above, only the technique of extracting only calcium from anchovy or adding a herbal ingredient has been disclosed, and no efficient extraction of calcium from anchovy has been disclosed.

In addition, studies on the absorption of calcium in the human body have not been disclosed in the above-mentioned conventional techniques, and no studies have been disclosed about inhibiting the aging activity such as antioxidant activity and bone formation.

The object of the present invention is to solve the above-mentioned problems, and it is an object of the present invention to establish an optimal extraction process for a functional ingredient from a natural herbal material and to provide an anchovy powder excellent in calcium source And an aging-friendly anchovy calcium agent.

Another object of the present invention is to confirm the safety and usability of the material and to improve the absorption rate of calcium in the living body, and also, based on the principle of oriental medicine, a high-quality anchovy Calcium.

In order to achieve the above object, the aging-friendly high-quality anchovy calcium agent using the herbal material according to the present invention is an aging-friendly high quality anchovy calcium agent using a herbal material, Wherein the herbal extract comprises 10 to 20% by weight of Sulfuric Acid, 10 to 20% by weight of Angelica gigantis, 10 to 15% by weight of bone turnover, 10 to 20% by weight of Gojiji, Wherein the anchovy powder comprises 10 to 15% by weight of mulberry, 3 to 7% by weight of wax, 3 to 7% by weight of broth, 3 to 7% by weight of speed, 3 to 7% by weight of licorice and 3 to 7% The dried anchovy powder contained 3.3 wt% of moisture, 26.6 wt% of crude protein, 1.6 wt% of crude fat, 66.0 wt% of crude fat and 2.5 wt% of carbohydrate. The dried anchovy powder was dried, The herbal medicine extract had a moisture content of 18.1% by weight, 7.5% by weight of crude protein, 0.9% by weight of crude fat, 5.5% by weight of whey protein and 68.0% by weight of carbohydrate.

In the anchovy calcium preparation according to the present invention, the herbal medicine extract was prepared by mixing 1 kg of a mixture of the above-mentioned Sukjipu, Angelica keiskei, Bombyx mori, Gugija, Bukchon, Wusu, broiler, The mixture was extracted at 105 ± 2 ° C for 7 hours, and then concentrated under reduced pressure in a rotary evaporator.

In the anchovy calcium preparation according to the present invention, the herb extract and the anchovy powder are mixed at a weight ratio of 1: 1.

As described above, according to the age-friendly high-quality anchovy calcium agent using the herbal material according to the present invention, an optimal extraction process for functional ingredients from a natural herbal material is established, and based on the herbal extract obtained from the herbal extract, It is possible to improve the functionality by mixing with an excellent anchovy powder and to provide an aging-friendly functional food.

According to the age-friendly high-quality anchovy calcium preparation using the herbal material according to the present invention, considering an entry into an aging society, a high morbidity rate of bone disease, and a high degree of interest of consumers, a high quality anchovy calcium preparation It is also possible to contribute to the public health and contribute to the increase of jobs through income generation of farmers and fishermen.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart of a conventional method of producing anchovy powder,
FIG. 2 is a view showing bilateral ovarian resection for artificially inducing osteoporosis by changing an experimental animal to a postmenopausal hormone secretion state in order to apply the present invention;
FIG. 3 is a graph showing changes in body weight in the Sham group, the OVX group, the OVX-AnCa group, and the OVX-AnCa-OM group when the 8-week body weight was measured at a constant time every day,
FIG. 4 is a graph showing bone mineral density in the Sham group, the OVX group, the OVX-AnCa group and the OVX-AnCa-OM group as measured by 8 SD-rats in each experimental group,
FIG. 5 shows the results of ANCA and ANCA-B in the femur weight (A), femoral ash (B), and femur calcium (C and D) of experimental rats in the Sham group, OVX group, OVX-AnCa group and OVX- Graph showing the effect of OM supply,
FIG. 6 is a graph showing osteocalcin concentration in serum according to administration of anchovy powder and combination herbal medicine extract in Sham group, OVX group, OVX-AnCa group and OVX-AnCa-OM group,
FIG. 7 is a graph showing APL concentration in serum of experimental rats of Sham group, OVX group, OVX-AnCa group and OVX-AnCa-OM group,
FIG. 8 is a graph showing the calcium concentration status in serum of experimental rats of Sham group, OVX group, OVX-AnCa group and OVX-AnCa-OM group,
9 is a graph showing the results of measurement of GOT and GPT in the sera of experimental mice of Sham group, OVX group, OVX-AnCa group and OVX-AnCa-OM group,
FIG. 10 is a graph showing HDL (A), LDL (B) and triglyceride (C) in the blood of experimental rats of Sham group, OVX group, OVX-AnCa group and OVX-AnCa-OM group.

These and other objects and novel features of the present invention will become more apparent from the description of the present specification and the accompanying drawings.

The age-friendly and high-quality anchovy calcium agent using the herbal material according to the present invention can be obtained by extracting herbal medicine extracts (herbal extract) of any one of medicinal herb extracts such as Seokjanghwang, Angelica gigas, Boksoobo, Gugija, And anchovy powder.

Ingredients of the anchovy ( Engraulis japonicus ) used for the calcium material in the aged-friendly high-quality anchovy calcium preparation using the herbal material applied to the present invention were purchased from Pukyong National Fisheries, Busan in October 2013. The purchased material residue was subjected to the processes of cooking, washing and dehydrating 35 times to remove foreign substances and sodium. Dried in a hot air drier, pulverized to a particle size of 500 mesh by a pulverizer, and packed in an experiment.

The oriental materials used for the analysis were oriental oriental materials which can help the geriatric osteoporosis based on the prescription of Oriental Medical College of Dong - Eui University. The herbal extracts were extracted with 1 kg of herbal ingredients and 4 L of water using a water-cooled cooling circulation extractor DM-1000 (Daehan Median Co., LTD, Seoul, Korea) and extracted at 105 ± 2 ° C for 7 hours. The extracts were concentrated under reduced pressure on a rotary evaporator Respectively.

The general components were measured according to the commercial method. That is, moisture was measured by atmospheric pressure heating drying method, semimicro Kjeldahl method for crude protein, Soxhlet method for crude fat, and dry method for inquiry. Carbohydrates were calculated as 100% of the total, excluding moisture, crude protein, crude fat and ash content.

The coliform count was calculated by counting the number of colonies forming bubbles around the red colonies produced after incubation at 35 ± 2 ℃ for 24 ± 2 ℃ in 3M medium for coliform counting and diluting multiples And the number of E. coli strains was calculated.

As for the determination of heavy metals, for the analysis of lead and mercury, Hazardous Substance Test Method ', the test solution was prepared by dry method and analyzed by inductively coupled plasma method. For the analysis of arsenic, the decomposition of the samples was carried out in foodstuffs' 7. Hazardous Substance Test Method ', the test solution was prepared using the wet method and analyzed by microwave method.

The quantitative determination of magnesium was carried out by wet decomposition using nitric acid (HNO ₃ ), and then by inductively coupled spectrophotometer (SPECTROBLUE ICP-OES (Radial, Axial, Twin Interface) The amount of sodium was determined by using ICP and SPECTROBLUE ICP-OES after the test solution was prepared by using the dry method of the sample, and the amount of phosphorus was analyzed by the molybdenum blue colorimetric method.

The calcium content was measured as follows.

Experimental animals were sacrificed 8 weeks after the administration of the sample and the femur was cut. The fat and surrounding tissues remaining in the femur were removed, washed with physiological saline, dried at 105 ± 5 ° C and weighed to calculate the body weights of the animals.

The dried femur was placed in the ash crucible and thoroughly stirred at 550 to 600 ° C for 12 hours. After 30 minutes of cooling, the ash was weighed. Concentrated ash was dissolved in 1N HCl, diluted with 1% La ₂ O ₃ , and calcium content was measured with an atomic absorption spectrometer (Perkin Elmer Co., Santa Clara, CA, USA).

In addition, the animals used for the experiment in the present invention were female Sprague-Dawley (SD) rats of 4 weeks old, purchased from Central Experimental Animals (Seoul, Korea).

The animals were acclimated for one week at the experimental animal facility of the Center for Development and Practical Use of Blue Bio Materials at Dong - Eui University and the normal symptoms were observed and healthy animals without abnormalities were selected.

The animals were kept at a temperature of 23 ± 2 ° C, a relative humidity of 50 ± 10%, a ventilation frequency of 10 to 18 times / hour, a lighting time of 12 hours (8:00 am to 8:00 pm) And sterilized radioactive sterilized animal feed and high pressure sterilized primary distilled water.

Animal experiments were conducted in accordance with the provisions of the Animal Experimental Ethics Committee under the prior deliberation of the Ethical Committee of Animal Experiments at Dong-Eui University.

Ovariectomy (OVX) was performed to artificially induce osteoporosis by changing experimental animals to postmenopausal hormone secretion. After sterilization, the skin and muscles and peritoneum of both sides were treated with 10% povidone iodine and the ovaries were exposed. The ovaries were removed and the resection site Were ligated and sutured with absorbable sutures.

The administration dose setting and the administration method were carried out as follows.

The animals were fasted for 4 hours before the oral administration and then weighed and divided into 8 experimental rats of 32 male Sprague-Dawley rats according to the random method according to the weight range. The dose of anchovy powder was determined with reference to the previous report (Kim, Hyang-sook, etc., 2013). The dose of the herbal extracts was 1: 1, And the dose of anchovy powder sample was set at 100 mg / day.

The dose determined according to the experimental group was administered orally using a stainless steel sonde for oral administration. Feeds were restricted for 2 hours after the test substance once daily, and negative water was continuously supplied without limitation. Anchovy powder (100 mg / day) was suspended in sterilized physiological saline and OVX-AnCa-OM group (2), anchovy powder and herbal medicinal herb extracts, were given in Table 3-4 The same dose was administered to 1 mL of physiological saline and suspended. The same amount of saline was administered to Sham group and OVX group. When the animals were unable to be administered at one time according to the individual characteristics of the animals, they were divided into two doses on the same day.

In the present invention, blood sampling and autopsy were carried out as follows.

The animals were fasted for 12 hours before sacrifice, and anesthetized with an appropriate amount of diethyl ether in a desiccator. The lower abdomen was opened and blood was collected by a cardiac syringe using a sterile syringe. After blood sampling, lesions of major internal organs were visually observed in each experimental group and control group to confirm the abnormality.

The sensory test was carried out as follows.

There were 6 items of texture, flavor, color, taste, feeling of irritation, and overall acceptance. The degree of preference was 1, the highest was 5 The results were evaluated by the range of the maximum 5 points and the minimum 1 point. Samples were cut into a size of 1.5 x 1.0 x 0.7 cm ^{3 in the} size, width, length and height in a disposable dish. The sample was evaluated 24 hours after the preparation.

The mean value of the results obtained by the above-mentioned experiment was compared with the mean standard deviation (mean SD). Statistical analysis was performed by Student's t-test and the P-value was judged to be significant when the value was less than 0.05.

The general components of the anchovy powder according to the present invention are 3.3% by weight of moisture, 26.6% by weight of crude protein, 1.6% by weight of crude fat, 66.0% by weight of ash and 2.5% by weight of carbohydrate.

Anchovy powder showed the highest content of ash content (66.0%) as the source of minerals, followed by crude protein (26.9%), Which is expected to increase the inorganic content. On the other hand, the content of crude protein was high, indicating that the anchovy powder could be used as a food material and utilized as a protein source.

As shown in the following Table 2, the extracts of Oriental herbal medicine extracts were 18.1% by weight of water, 7.5% by weight of crude protein, 0.9% by weight of crude fat, 5.5% by weight of ash and 68.0% by weight of carbohydrate.

The contents of carbohydrate were 68.0%, followed by moisture, crude protein, crude fat and crude fat.

Table 3 shows the results of analyzing the mineral content of anchovy powder.

Calcium, 24.4g / 100g ash, phosphorus 13.3g / 100g ash, and sodium, potassium and magnesium were contained in a small amount as 0.10.5g / 100g ash. In the case of heavy metals, lead (0.1 mg / kg ash), arsenic (0.1 mg / kg ash) and mercury (not detected 0.0 mg / kg ash) The anchovy was caught in the southern coast known as the clean sea area, and it was judged that the pollution degree was small.

Table 4 shows the results of analyzing the mineral content of the herbal extracts.

It was composed of calcium 4.3mg / 100g ash and phosphorus 11.2mg / 100g ash though it was a small amount compared with calcium powder, and magnesium contained 8.1mg / 100g ash in trace amount. In the case of heavy metals, lead (0.1mg / kg ash) and mercury (0.0mg / kg ash not detected) were not detected, so it was analyzed that the safety of raw materials was not a problem at all. It is considered that there is no big problem in food safety because it selects the herbal materials which passed both the domestic and imported standards.

Lead (Pb) lead contamination is accumulating due to contamination of soil by insecticide pesticides (Kim Duk-woong, 2005). Lead poisoning is known to cause nervous, smooth muscle disorders and hemoglobin in red blood cells, leading to anemia, causing chronic symptoms such as acute toxic symptoms such as anemia, cerebral palsy, kidney disorder and pale skin, headache, and loss of appetite Concon, 1988; Choi, 1995; Conor, 1991). It was detected as 3 times lower than the standard value as 0.3 mg / kg, which is the limit value of lead in calcium given in the standard of health functional foods.

Arsenic (As: Arsenic) is especially found in soils, water, and plants and animals, but the form contained in foods is known to be mostly toxic organic arsenic (Yu, Chuncheol et al., 2005). Ingestion may cause vomiting, hematuria, diarrhea and prolonged exposure may cause anorexia, weight loss, facial edema, obstructive jaundice, neuritis or skin keratosis (Kim, Mi Hye et al., 2004.). Anchovy powder and herbal extracts showed low level contents.

In the case of mercury (Hg: Mercury), metals used in cosmetics, medicines, and paints are contaminated in mercury mines, chemical plants, amalgam, thermometers, and mercury batteries. Today, contamination sources of agricultural products include mercuric pesticides Soil contamination, industrial wastewater, and urban sewage into farmland. Minamata disease, an organic mercury poisoning in Japan, is known to cause central nerve paralysis due to fish and shell contamination. According to the WTO data, the mercury content present in the form of an inorganic substance in food is less than 20 g / kg (Concon, 1988; Choi, 1995, Conor, 1991). The anchovy powder and the herbal extract applied to the present invention were not detected, and the results of the analysis were satisfactory enough to satisfy the problem of safety by heavy metals.

The coliform group was negative in both herbal extracts and anchovy powder, and the safety criteria of coliform group was secured by satisfying the criteria set forth in the standard of calcium in the functional food of the health food.

Next, the establishment of an optimal extraction method of the functional ingredient of the herbal material according to the present invention will be described.

Based on data from Dong Eui University and Blue Bio Material Development and Practical Area Innovation Center, we selected herbal ingredients that can help with osteoporosis. The herbal extracts were extracted with 1 kg of herbal ingredients and 4 L of water using a water-cooled circulation extractor (DM-1000, Daehan Median CO., LTD, Seoul, Korea) and extracted at 105 ± 2 ° C for 7 hours. Respectively. The oriental herbal materials used were mixed with oriental herb, which is known for the prevention and treatment of bone disease, such as Sukjunghwang, Angelica gigas, Bogwoji, Gugija, Giugi, Daejang,

The method of preparing the herbal extract and anchovy powder according to the present invention is as follows.

In the present invention, since postmenopausal osteoporosis has a direct relationship with the physical phenomenon of menopause, approach to the treatment of osteoporosis in postmenopausal women was approached from the macro perspective of aging.

Menopause is the complete termination of menstruation, and menopause is usually around age 49. In menopause, the uterus or ovaries are removed artificially. 75% of menopausal women have no symptoms, but in about 25%, symptoms of vasomotor system such as flushing, burning sensation, insomnia, Urticaria, osteoporosis, genitourinary atrophy, and fatigue due to imbalance of the autonomic nervous system, depression, dizziness, flushing, insomnia, discomfort, loss of concentration, dyspnea, emotional anxiety, and tension.

In medical treatment, estrogen is preferentially prescribed in the absence of special contraindications such as estrogen dependent tumors, breast cancer, unexplained uterine bleeding, uterine cancer, stroke, arteriosclerosis, thromboembolic disease, and the like. The prevalence of osteoporosis is estimated to be about 75 out of 1,000 women in their 40s who did not receive estrogen, but to about 25 after the administration of estrogen.

Estrogen therapy involves a risk, which is the carcinogenicity of estrogen. Although progestin therapy has been recommended several years ago, it has recently been reported to exacerbate breast cancer (Schairer et al., 2000; Stephens et al., 2000).

Therefore, in Oriental gynecology, osteoporosis is regarded as a type of chronic menopausal disorder and is dealt with in the category of postmenopausal disease. Therefore, it has been reported that when the kidney and liver are weakened due to the general physical changes of menopause, gynecologic diseases come to be used with the addition of the Korean herbivorous ginseng and the feathers.

In addition, a combined herbal extract recipe such as a table was prepared by collecting reports on the use of single or two herbal medicines and calcium, and published papers, patents, and reports on osteoporosis.

Extract powder was prepared by blending medicinal herbs with main ingredients such as Sukjanghwang, Angelica gigas, Bokseongbo, Gugija, Quail, Wassle, Broiler, Velvet, Licorice, The specific compounding ratios are shown in Table 5.

Sukjipheong belongs to the genus Scrophulariaceae ( Rehmannia There is glutinosa . purpurea), which has been shown to be effective for osteoporosis, which has lost its reproductive function and aging, has been used as a herb. It has been known that tyrosine, ajugol, 8-epi-Loganic acid, Alanine, Aucubin, Catalpol, Glutamic acid, Isoleucine, Rehmaionoside Among these, aucubin promotes collagen synthesis.

Angelica is a herbaceous plant belonging to the family, and can be used effectively as a substitute for windsurfing for women. Roots are used as medicinal materials. The main components of the roots are pyranocoumarin type decursin and decursinol, AChE inhibitory action, and aromatic essential oil, saccharides and vitamin E. Niwa et al. (2001) reported that angelfish brefeldin A inhibited osteoclastic bone resorption by inducing osteoclast apoptosis by a p53-dependent mechanism.

It is said to be effective in relieving inflammation such as inflammation, tonic action, bruise, etc. It suppresses pain and strengthens muscles and bones by drying roots of gonorrhoeae. Yamaguchi et al. (1999) reported that bone grafting significantly inhibited bone loss in osteoporotic osteoporotic mice (Yamaguchi K., 1999). It is also known that the hernia can be strengthened not only in the genitourinary system but also in the muscularis by viewing Xinyang as a medicinal herb.

The Gugija belongs to the family Gugija ( Lycium chinense , L. barbarum ) ( Lycii fructus ). In herbal medicine, phenol and sterols, enol, betaine, diosgenin, kukoamine A, lyciumamide, lyciumin, stigmasterol, sugiol, beta-amyrin and beta-sitosterol have been tested as chemical components. There are studies on hepatocyte protection and radiation protection, all of which have been shown to be due to the regenerative action of damaged and senescent cells.

It is a dried bark of Eucommia ulmoides , which belongs to the genus Duchamp . It protects the liver and kidneys, and has the effect of preventing musculoskeletal diseases, so it plays an important role in maintaining skeletal function and hormone function. It has been found that the chemical components are ajugoside, aucubin, betulin, caffeic acid, eucommioside III, genipin, geniposide, geniposidic acid, syringin, syringoside, alyposide, ligustrin and beta-Sitosterol. Liu et al. (1998) reported that geniposidic acid and aucubin (Monoterpenoid) act to strengthen muscles and bones by promoting collagen synthesis.

The whistle is called the Achyranthes bidentata , A. japonica ). The efficacy is efficacious for aging bone disease by eliminating eosinophilia, analgesic effect, protection of liver and kidney, and efficacy to prevent musculoskeletal diseases. The chemical components are tyrosine, arginine, aspartic acid, glutamic acid, inokosterone, leucine, oleanolic acid, phenylalanine, proline and serine. Xie et al. (2001) reported that SOD activity was increased and ALT and AST were lowered to remove oxygen radicals, and thus, it has antioxidant and anti-aging activity, and promotes blood circulation and eosinophilia to restore chronic liver damage.

Broiler cabbage belongs to the camphoraceae family, and it is a medicine that dried the bark and warms the stomach to strengthen muscles and bones, and it has the effect of improving absorption of all medicines through improvement of blood flow. Broilers also have a pharmacological effect such as central suppression, sedation, analgesia, fever, increased blood flow to the coronary arteries, and weakness in the back and knee.

The genus is the root of the conifers belonging to the Lamiaceae family, and it is defined as Phlomis umbrosa in the Korean herbal medicine standard collection. The chemical component is betonicine, which has anti-inflammatory activity and contains succinic acid and asperosaponin FH1, cantleyine, daucosterol, venoterpine, and -sitosterol. It has efficacy in prevention of urogenital dysfunction caused by aging, weakening of skeleton, fracture.

Glycyrrhiza belonging to the soybean is the main component of licorice, Glycyrrhiza is the major component of the triterpenoid saponin, glycyrrhizin and the main component of degradation products, such as glucuronic acid has the effects of detoxification, antioxidant, antiinflammatory and corticosteroid hormones, It can be used as a material to minimize burdens and side effects.

It has long been reported that the roots are long and have many twigs so that they strengthen the back and legs with the same name as the spine bones of the dogs and facilitate the movement of the joints and thus have efficacy for back pain, arthritis and lower incompetence (Jong Hee Park, 2009).

Next, the skeletal metabolism of the herbal extract and anchovy powder mixture according to the present invention was evaluated.

(1) in vivo using animals Sprague-Dawley (SD) rat induced osteoporosis in ovariectomized test

1) Experimental animals

The animals used in the experiment were female Sprague-Dawley (SD) rats of 4 weeks old, purchased from Central Laboratory Animals Co., Ltd. (Seoul City, Korea).

The animals were acclimated for one week at the experimental animal facility of the Center for Development and Commercialization of Blue Bio Materials at Dong - Eui University and the normal symptoms were observed.

The animals were kept at a temperature of 23 ± 2 ° C, a relative humidity of 50 ± 10%, a ventilation frequency of 10 to 18 times / hour, a lighting time of 12 hours (8:00 am to 8:00 pm) And sterilized radioactive sterilized animal feed and high pressure sterilized primary distilled water.

Animal experiments were conducted in accordance with the provisions of the Animal Experimental Ethics Committee under the prior deliberation of the Ethical Committee of Animal Experiments at Dong-Eui University.

2) Ovarianectomy and classification

In order to artificially induce osteoporosis by changing the experimental animals to postmenopausal hormone secretion state, ovariectomy (OVX) was performed as shown in FIG. FIG. 2 is a view showing a bilateral ovariectomy for artificially inducing osteoporosis by changing an experimental animal to a postmenopausal hormone secretion state in order to apply the present invention.

As shown in FIG. 2, after sterilization, the surgical site and surrounding area were disinfected with 10% povidone iodine, and then skin, muscle, and peritoneal incisions were performed on both sides of the ovary to expose both ovaries The ovaries were removed and the ablation site was ligated and sutured with an absorbable suture.

(OVX-AnCa), ovariectomized group (OVX-AnCa), ovariectomized group (OVX-AnCa), ovariectomized group (OVX-AnCa), and ovariectomized group were excluded from ovariectomy. After ovariectomy, as shown in Table 6 and Table 7, they were divided into four groups such as anchovy powder + herbal medicine group (OVX-AnCa-OM).

3) Extraction and preparation of test materials

The medicinal herbs used in the experiment were purchased from Korean herbal medicine products (Busan city). The same dry weight as that of Table 5 was added, and 4 times of distilled water was added, followed by hot extraction at 105 ± 2 ° C. for 7 hours. The extract solution was a nonwoven fabric and Whattman paper No. 1. And then concentrated under reduced pressure. After lyophilization using a freeze drier (FDU-2000, EYELA, Japan), the herbal extract powder was obtained. The anchovy powder was supplied by JISPI Co., Ltd. Anchovy powder and herbal extracts were mixed uniformly at a ratio of 1: 2 (g / g) and stored in a freezer at -80 ℃ for use as a sample.

4) Setting dose and method of administration

The mice were fasted for 4 hours before the forced oral administration and the body weights were measured. Then, the female Sprague-Dawley (SD) rats were randomly divided into 8 experimental animals of 32 animals each according to the weight range Respectively.

The dose of anchovy powder was determined with reference to the previous report (Kim, Hyang-sook, etc., 2013). The dose of the herbal extracts was 1: 1, The dose of 200 mg / day anchovy powder was set at 100 mg / day. The dose determined according to the experimental group was orally administered using a stainless steel sonde for oral administration. Feeds were restricted for 2 hours after the test substance once daily, and negative water was continuously supplied without limitation. Anchovy powder (100 mg / day) was suspended in sterilized physiological saline and OVX-AnCa-OM group 2 was administered to the experimental group 1 OVX-AnCa group. Was suspended in 1 mL of physiological saline and administered. The same amount of saline was administered to Sham group and OVX group. When the animals were unable to be administered at one time according to the individual characteristics of the animals, they were divided into two doses on the same day.

5) General symptom observation and weight measurement

On the day of dosing, the presence or absence of abnormal symptoms was observed continuously for 30 minutes immediately after administration. Changes in the general state, manifestation of poisoning symptoms, and the like were observed at a certain time once a day from day 1 to day 14, Presence of dead animals and other abnormal symptoms were observed.

Observations of changes in body weight were performed on all animals for 12 hours and then measured once a week using a laboratory animal scale at the normal time of day.

The 8-week body weight was measured at regular intervals over a period of time. As a result, the body weight of the Sham group was increased normally while the OVX group, which was ovariectomized and ovariectomized, continued to gain weight. The OVX-AnCa group and the OVX-AnCa-OM group, which were two experimental groups after OVX treatment, showed a significant difference in weight loss as shown in FIG. 3 as compared to the OVX-AnCa-OM group . FIG. 3 is a graph showing changes in body weight in the Sham group, the OVX group, the OVX-AnCa group, and the OVX-AnCa-OM group as a result of measuring the body weight for 8 weeks at a constant time every day.

The ovariectomized experimental animals are known to have an overall increase in body weight due to estrogen deficiency (Mobasseri et al., 2004). The weight gain of the OVX group, which has been induced by ovariectomy to induce osteoporosis, is consistent with the phenomenon of weight gain in these menopausal women. On the other hand, relative weight loss in the two experimental groups disproved the improvement in osteoporosis associated with these menopausal years.

6) Blood collection and autopsy

The animals were fasted for 12 hours before sacrifice, and then anesthetized with an appropriate amount of diethyl ether in a desiccator. The lower abdomen was opened and blood was collected by a cardiac syringe using a sterile syringe.

After blood sampling, lesions of major internal organs were visually observed in each experimental group and control group to confirm the abnormality.

No significant abnormalities were observed in the major internal organs in all four experimental groups, but visceral fat was relatively higher in the OVX, OVX-AnCa, and OVX-AnCa-OM groups than in the Sham group.

Next, bone mineral density was measured.

After 8 weeks of administration, the experimental animals were sacrificed and both femurs were separated and removed. The muscle, ligament and fat attached to the skeleton were removed, washed with physiological saline, and dried at 50 ° C to prepare a femur bone sample.

The bone mineral density of the femur was measured using a pDEXA X-ray bone densitometer (Norland medical systems Inc., USA) for small animals. The resolution was 1.0 × 1.0 mm, the speed was 20 mm / sec, the length of the sample was 1.60 cm, and the width was 3.90 cm.

In the experimental group, 8 SD-rats showed relatively low bone density in the Sham group and OVX group. In the two experimental groups such as OVX-AnCa and OVX-AnCa-OM, 9 and Fig. 4, the increase in bone density was statistically significant. FIG. 4 is a graph showing the bone mineral density in Sham group, OVX group, OVX-AnCa group and OVX-AnCa-OM group as measured by 8 SD-rats in each experimental group.

Sham group had 0.186 g / ㎠ and OVX group with osteoporosis showed average 0.170 g / ㎠. However, t-test showed significant difference. This suggests that ovariectomy results in osteoporosis in the OVX group and a significant decrease in femur bone mineral density.

On the other hand, mean OVX-AnCa group showed 0.270 g / ㎠ bone density and OVX-AnCa-OM group showed 0.310 g / ㎠ bone density. The OVX-AnCa-OM group showed the highest bone mineral density compared to the OVX group in both experimental groups.

Other reports of effects on osteoporosis, such as isoflavones, osteoblasts, osteoblasts, gelatins, etc., show a decrease in bone mineral density relative to the normal group in the case of osteoporosis-induced experimental animals, The results of this study are as follows: (1) In the case of an experimental group, the trend is consistent with the increasing phenomenon (Kim, Kee-Yup, et al., 2009;

Next, bone calcium content was measured.

Experimental animals were sacrificed 8 weeks after the administration of the sample and the femur was cut. The fat and surrounding tissues remaining in the femur were removed, washed with physiological saline, dried at 105 ± 5 ° C and weighed to calculate the body weights of the animals.

The dried femur was placed in the ash crucible and thoroughly stirred at 550 to 600 ° C for 12 hours. After 30 minutes of cooling, the ash was weighed. Concentrated ash was dissolved in 1 N HCl, diluted with 1% La ₂ O ₃ , and calcium content was measured with an atomic absorption spectrometer (Perkin Elmer Co., Santa Clara, CA, USA).

Dry weight of the femur was significantly lower in the OVX group and slightly higher than that of the Sham group in the two experimental groups. This return of the femur weight to the pre-osteoporosis state is consistent with previous reports of a similar phenomenon in the supply of calcium lactate, bovine calcium, and calcium carbonate.

Changes in the ash content of the femur were 299.6 mg in the OVX group and 317.8 mg in the Sham group. On the other hand, as shown in Table 10 and FIG. 5, in anchovy powder administration group or mixed herbal medicine powder administration group, 323.0 mg and 319.6 mg were increased compared with OVX group and Sham group, which showed a tendency to be proportional to femur dry weight. FIG. 5 shows the results of ANCA and ANCA-B in the femur weight (A), femoral ash (B), and femur calcium (C and D) of experimental rats in the Sham group, OVX group, OVX-AnCa group and OVX- As a graph showing the effect of OM supply, all values are average SD.

The calcium content in the femoral ash was also similar in the OVX group, compared with 141.8 mg in the normal group Sham group and 144.2 mg and 46.3 mg in the 2 experimental group, respectively. This was similarly observed in the ratio of calcium weight in the ash to the dry weight of the femur as a ratio, and it was proved that the two samples used in the present invention are effective for femur weight and calcium increase.

Next, a biochemical analysis of skeletal metaplasia-related indicators by anchovy calcium according to the present invention was carried out .

Osteocalcin is an acidic protein in the skeleton that is produced only in osteoblasts and ovarian cells. Osteocalcin concentration in serum is known to reflect the activity of osteoblasts (Jayachandran and Miller, 2002). Osteocalcin is tightly bound to calcium in the bone structure, which, when forming new bone, also serves as an indicator of bone formation that indirectly reflects the activity of the bone cell. It has been reported that ovariectomy results in a somewhat reduced serum content (Duda et al., 1998).

In the present invention, the osteocalcin content in the serum of SD-rats was measured using a 96-well plate and an automatic biochemical analyzer (BS-390, USA) with the Rat Gla-Osteocalcin High Sensitive EIA Kit (Cat # MK126, Clontech Laboratories, And were measured by immunoassay using automated biochemistry analyzer, Medista, Korea).

In the OVX group, the lowest level was 1.23 ng / mL, and the Sham group was 1.57 ng / mL, which was significantly higher than the OVX group. The experimental groups OVX-AnCa and OVX-AnCa-OM were increased compared to OVX but lower than Sham. These results indicate that administration of anchovy powder and administration of the herbal medicine extract may increase the concentration of osteocalcin in the serum. As shown in Table 11 and FIG. 6, it is presumed that the sample administration according to the present invention activates the osteoblasts in osteoporosis caused by ovariectomy and decreases the activity of bone destruction cells. 6 is a graph showing the concentration of osteocalcin in serum according to administration of anchovy powder and combination herbal medicine extract in Sham group, OVX group, OVX-AnCa group and OVX-AnCa-OM group.

Osteocalcin is a protein that is specifically present in bone and dentin, and is known to be most sensitive to osteoblast activity. Osteocalcin is produced in osteoblasts, some of which are accumulated in bone matrix and some are released into the blood (Price et al , 1980). During osteogenesis, osteoblast activity is increased and serum osteocalcin concentration is used as an index of bone formation. In addition, the concentration of osteocalcin is increased after menopause and is used to predict the rate of bone loss after menopause (Ghattahan et al., 2006). In other words, the increase in serum osteocalcin concentration is interpreted as a positive aspect of increased bone formation in younger age group, but it is reported to have a negative correlation with bone mineral density after menopause, which means an increase in bone turnover rate.

The results of the experiment according to the present invention show that administration of anchovy powder and administration of the herbal medicine extract can increase the concentration of osteocalcin in the serum. It is presumed that the administration of the sample of the present invention activates the osteoblast in osteoporosis bone caused by ovariectomy and decreases the activity of bone destruction cell. In the anchovy powder administration group, it was similar to that of normal group Sham group but increased in the experimental group treated with anchovy powder and herbal medicine extract similar to the normal value. This indicates that synergistic effects can be obtained when the herb extract is administered together.

Next, the physiological activity of the herbal extract and anchovy powder mixture according to the present invention was evaluated.

(1) Analysis of biological function index in blood

Alkaline phosphatase (ALP), calcium, GOT, and GPT in the blood of experimental animals were analyzed.

The blood collected from the heart of the experimental animals was centrifuged at 3,000 rpm at 4 ° C for 15 minutes (Avanti centrifuge J-20XP, Beckman coulter, USA).

ALP assay kit (Asan Co. Ltd., Korea) was used for serum isolated from blood and quantified at 500nm using spectrophotometer.

HDL-cholesterol, LDL-cholesterol, triglyceride, GOT, GPT and Ca were measured and analyzed by immunoassay using an automatic biochemistry analyzer (Medista, Korea).

 ALP is the most commonly used bone formation marker in clinical practice, and is a glycoprotein enzyme that is produced during osteoblast formation and some of which is secreted into the blood. After ovariectomy, the activity of ALP was reported to increase (Morris, 1993). Serum ALP levels were higher in the ovariectomized OVX group than in the Sham group after 8 weeks of treatment. Meanwhile, as shown in Table 12 and FIG. 7, in the experimental group in which the anchovy powder and the herbal medicine extract were administered, the values were 110.27 U / L and 108.81 U / L, respectively, which were lower than those of the Sham group. FIG. 7 is a graph showing APL concentration in serum of experimental mice of Sham group, OVX group, OVX-AnCa group and OVX-AnCa-OM group.

A change in serum calcium concentration is also one of the bone resorption indexes. Calcium in the bone affects calcium concentration in the serum, and changes in serum calcium concentration are related to bone resorption (James et al., 1994). Calcium content in serum was significantly decreased in OVX group compared to Sham group. In the experimental group administered with the test substance, the value increased compared to the OVX group and the Sham group. This means that, as shown in Table 12 and FIG. 8, the bone regeneration activity is relatively active in the experimental group. FIG. 8 is a graph showing the calcium concentration status in serum of experimental rats of Sham group, OVX group, OVX-AnCa group and OVX-AnCa-OM group.

GOT and GPT are abundant in the liver and heart, and are enzymes that degrade amino acids. When hepatocytes are damaged, they are released into the blood, and these levels are elevated, which is indicative of a hepatic disease (Kim, Hansoo, 2010). In the experiment of the present invention, GOT and GPT in the serum were measured to determine the effect of the herbal medicine extract on the long-term intake of the herbal extract. As a result, the value of GOT in the general control group was 120.61 U / L and GPT were 48.29 U / L, respectively. The OVX group in ovariectomized ovariectomized rats was 242.63 U / L and 59.63 U / L, respectively. The GOT was doubled and the GPT was increased by 20% . On the other hand, both GOT and GPT values in the OVX-AnCa and OVX-AnCa-OM groups were significantly decreased in the two experimental groups fed the experimental samples. Although the 8-week administration did not reduce the Sham group to that of the normal group, as shown in Table 12 and FIG. 9, the OVX group was significantly lower than the OVX group, and statistically significant Respectively. FIG. 9 is a graph showing the results of measurement of GOT and GPT in sera from experimental rats of Sham group, OVX group, OVX-AnCa group and OVX-AnCa-OM group. These results are interpreted as a result of lowering the number of livers of osteoporotic animals, although the experimental samples may have an effect on the liver depending on the concentration.

Next, the parameters of HDL-, LDL-cholesterol, and triglyceride among the lipid components in the blood of the experimental animals were analyzed.

The characteristics of lipid components in blood of osteoporosis-induced rats fed with anchovy powder and herbal medicine extracts are shown in Table 13 and FIG. FIG. 10 is a graph showing HDL (A), LDL (B) and triglyceride (C) in the blood of experimental rats of Sham group, OVX group, OVX-AnCa group and OVX-AnCa-OM group.

The ovariectomized OVX group showed a significant increase in HDL-cholesterol concentration compared with that of the normal group Sham, and was also significantly increased in the test group administered with anchovy powder and herbal medicine extract. LDL-cholesterol concentration was decreased in the two experimental groups. This resulted in elevation of serum HDL-cholesterol concentration in the ovariectomized rats by feeding the swine gelatinase hydrolyzate (Park, Jeong Eun et al., 2012).

Triglyceride showed a significantly increased value (92.24 mg / dL) in the OVX group compared to the Sham group. In the experimental group, the decrease was not significant but the decrease was significant in the group treated with herbal medicine extracts. In contrast, the ovariectomized group showed a tendency to decrease compared with the normal group (Tang et al., 2008; Park, Chan-soo, 2008) .

Next, the antioxidant activity of the herbal medicinal herb extracts was analyzed by in vitro assay.

1) DPPH radical scavenging activity measurement

DPPH has chemically stable free radicals. When it comes into contact with a substance with antioxidant activity, the radical is extinguished by giving electrons and the color changes from purple to yellow. The antioxidative capacity of the herbal extracts was measured using DPPH radical scavenging ability.

0.8 mL of 0.4 mM DPPH solution dissolved in ethanol was added to 0.2 mL of the sample, followed by shaking for 5 seconds with a vortex mixer, followed by 10 minutes of reaction in a dark place. Absorbance was measured at 517 nm using the following microplate reader. The DPPH radical scavenging activity was expressed as a percentage of the absorbance reduction of the sample solution added and the non-added sample.

Compared with ascorbic acid, a vitamin C antioxidant, the herb extracts of the present invention exhibited antioxidative activities of about 46 to 54% and showed a tendency to increase in a concentration-dependent manner. On the other hand, as shown in Table 14, the anchovy powder showed slight activity.

Compared with the conventional anchovy powder alone, the extracts of the herbal medicine extracts showed a strong antioxidative activity of more than 40% at 2 mg / mL, and the increasing tendency was concentration - dependent.

 Compared with anchovy powder alone, the antioxidant activity was increased by about 30% when the antioxidant activity was compared with the ratio of 1: 2 administered in the present study. The antioxidant activity of the herbal extracts was lowered by about 15% than that of the herbal extracts alone, but it was analyzed to be due to the small absolute amount administered to the ratio of 1: 2.

2) Measurement of SOD-like activity

It is an abbreviation of superoxide dismutase (enzyme) that removes active oxygen. It is an experiment to see if there is any substance that acts to neutralize this excess oxygen when it is necessary.

Add 0.2 mL of Tris-HCl buffer (50 mM tris-containing 10 mM EDTA, pH 8.5, 3 mL) and 7.2 mM pyrogallol (0.2 mL) to each 0.2 mL of each concentration and incubate at 25 for 10 min. Add 1 N HCl After stopping the reaction, the absorbance at 420 nm was measured and expressed as a percentage.

As in the case of DPPH radical scavenging, as shown in Table 15, the SOD results showed that the herbal extract extract had a concentration-dependent antioxidative activity and about 5053% of the ascorbic acid activity.

Also, when compared with the conventional anchovy powder alone, the antioxidant activity was more than 30% at 2 mg / mL, and the increasing tendency was concentration dependent. Anchovy powder alone showed a slight SOD - like activity.

When the antioxidant activity was compared at the ratio of 1: 2 administered in the present invention, the antioxidant activity was improved by about 20% or more as compared with the case of anchovy powder alone. The antioxidant activity of the herbal extracts was lowered by about 10% than that of the herbal extracts alone.

In view of the fact that osteoporosis is a disease that comes with aging phenomenon in recent medical reports and oriental women's science, it is anticipated that the herbal medicine extract having antioxidant activity will have anti-aging activity in addition to its own activity to overcome osteoporosis. do. In addition, synergistic effects on the prevention and improvement of osteoporosis can be expected in combination with anchovy powder having high absorption rate.

Next, prototype preparation and quality inspection of the herbal extract and anchovy powder mixture according to the present invention were carried out.

(1) Prototype production and sensory evaluation

Based on the above results, prototypes were prepared and sensory tests were conducted. In order to evaluate the effect of the anchovy calcium supplementation on the sensory evaluation, the subjects were selected from the students of Dong - They should be familiar with the purpose and detail information of the sensory evaluation experiment and evaluate the texture, flavor, color, taste, feeling of irritation, and overall acceptance Respectively. After sampling one sample, the sample was rinsed with water and then another sample was sampled and evaluated. The test method is classified into "very good (5 points), good (4 points), normal (3 points), poor (2 points) and very poor (1 point) I found the condition.

As shown in Table 16, the herbal extracts of the present invention were highly evaluated in the calcium preparations which were mixed with the calcium compounds rather than the conventional calcium compounds in all ages. Especially, it was highly evaluated by the elderly age group, and it was judged as a result of comfortable recognition and familiarity with oriental herbal materials.

Based on the evaluations received from all ages, it will be possible to develop functional materials suitable for the age group and develop differentiated formulations, differentiated packaging methods and designs.

Based on the positive evaluation of the herbal materials according to the present invention, it is expected to create various added value for the oriental herbal materials, and development of functional materials is expected with the positive image of food.

Although the present invention has been described in detail with reference to the above embodiments, it is needless to say that the present invention is not limited to the above-described embodiments, and various modifications may be made without departing from the spirit of the present invention.

By using the age-friendly high-quality anchovy calcium agent using the herbal material according to the present invention, an excellent high-function calcium agent can be provided.

Claims (3)

As an aged-friendly, high-quality anchovy calcium agent using herbal ingredients,
Anchovy powder and an extract of medicinal herbs consisting of a medicinal herb, a medicinal herb, a medicinal herb,
The medicinal plant extract according to claim 1, wherein the medicinal herb extract comprises 10 to 20% by weight of Sulfuric Acid, 10 to 20% by weight of Angelica keiskei, 10 to 15% by weight of bone marrow, 10 to 20% by weight of Gugija, 10 to 15% 3 to 7% by weight, 3 to 7% by weight, 3 to 7% by weight licorice and 3 to 7% by weight,
The anchovy powder was obtained by drying the anchovy residue after lyophilization, washing, dehydration, and pulverization at 500 mesh,
The anchovy powder comprises 18.1% by weight of moisture, 7.5% by weight of crude protein and 0.9% by weight of crude fat. The anchovy powder contains 3.3% by weight of water, 26.6% by weight of crude protein, 1.6% by weight of crude fat, 66.0% by weight of query and 2.5% by weight of carbohydrate. By weight, 5.5% by weight of whey protein, and 68.0% by weight of carbohydrate. The method of claim 1,
The herbal medicine extract was prepared by adding 1 kg of a mixture of Sukjunghwang, Angelica keiskei, Bombyx mori, Gugija, Jugwort, Wusu, broiler, herbaceous, licorice and cormorant and 4 L of water at a temperature of 105 ± 2 ° C for 7 hours using a water- Wherein the anchovy calcium concentrate is concentrated under reduced pressure in a post-rotary concentrator. 3. The method of claim 2,
Wherein the herbal medicine extract and the anchovy powder are mixed at a weight ratio of 1: 1.

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