KR20170025806A - C Composition for Kimchi Enriched Vitamin C and having Anti-Oxidant Whitening Effect of Kimchi Producing Method Using Thereof - Google Patents
C Composition for Kimchi Enriched Vitamin C and having Anti-Oxidant Whitening Effect of Kimchi Producing Method Using Thereof Download PDFInfo
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- KR20170025806A KR20170025806A KR1020150122786A KR20150122786A KR20170025806A KR 20170025806 A KR20170025806 A KR 20170025806A KR 1020150122786 A KR1020150122786 A KR 1020150122786A KR 20150122786 A KR20150122786 A KR 20150122786A KR 20170025806 A KR20170025806 A KR 20170025806A
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- kimchi
- vitamin
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Abstract
Description
본 발명은 비타민C를 강화시킨 김치조성물 및 이를 이용한 항산화활성 및 미백효과의 기능성김치의 제조방법에 관한 것이다. 보다 상세하게는 황태 육수와 청국장에 비타민C, 청각, 녹차분말, 및 고춧가루를 섞어 비타민C를 강화시킨 김치조성물을 만든 다음, 숙성시키는 단계와, 새우젓, 멸치액젖, 마늘, 생강, 양파, 기능성당 및 상기의 비타민C를 강화시킨 김치조성물을 혼합하여 김치양념을 만드는 단계와, 절인배추에 상기의 비타민C를 강화시킨 김치조성물이 혼합된 김치양념으로 기능성김치 담기하는 단계와, 상기의 기능성김치를 숙성 및 발효하는 단계로 구성되는 황태육수와 비타민C를 포함하는 김치조성물 및 이를 이용한 기능성김치의 제조방법에 관한 것이다.The present invention relates to a kimchi composition reinforced with vitamin C, and to a method for producing functional kimchi having antioxidative activity and whitening effect using the composition. More specifically, the present invention relates to a method of preparing a kimchi composition comprising vitamin C, hearing, green tea powder, and red pepper powder mixed with vitamin C in Hwangtae juice and chungkukjang, followed by aging, and a step of fermenting shrimp, anchovy milk liquor, garlic, Preparing a kimchi sauce by mixing the above-mentioned vitamin C-enhanced kimchi composition, adding a functional kimchi to the pickled Chinese cabbage in a kimchi sauce mixed with the above-mentioned vitamin C-enhanced kimchi composition, A fermented kimchi composition comprising fermented broth and vitamin C, and a method for producing a functional kimchi using the same.
본 발명의 비타민C가 강화된 기능성김치는 김치의 시어짐과 연부 현상을 방지할 수 있으며, 상큼하고 시원한 맛의 김치를 제공할 수 있다. 또한 항산화활성과 미백효과를 제공한다. The functional vitamin K-enhanced functional Kimchi of the present invention can prevent the sagging and the edge phenomenon of the kimchi and provide a fresh and cool taste of kimchi. It also provides antioxidant activity and whitening effect.
김치(Kimchi)는 무, 배추 및 오이 등을 소금에 절여서 고추, 마늘, 파, 생강, 젓갈, 당류, 소금 등의 양념을 버무린 후, 김치를 담는다. 김치는 숙성중에 발효에 의해 젖산균이 젖산을 비롯한 각종 유기산을 생성시키므로 상큼하고 새콤한 맛있는 김치가 만들어진다. 김치는 발효식품으로 각종 무기질과 비타민이 풍부해 영양학적으로 우수하며, 칼로리가 적고, 섬유질이 많으며, 김치 속 양념재료 중 고추의 주성분인 캡사이신에 의해 체내에서 지방질을 분해하여 제거하는 효과가 알려지면서 기능성 식품으로 알려져 있다. 젓갈은 아미노산으로 분해되어 단백질 공급원이 되고, 채소는 칼슘, 구리, 인, 철분, 소금 등의 무기질이 풍부하여 비타민 B의 흡수를 돕는다. 김치의 채소류는 즙과 식염등의 복합작용으로 장내를 깨끗하게 해주고, 위장내의 단백질 분해효소인 팩틴 분비를 촉진시키며, 장내 미생물분포를 정상화시킨다. 또한, 섬유소는 변비를 예방하고 장염이나 결장염 같은 질병을 예방해 준다. 젖산균에 의해 정장작용을 하고 소화를 도와주며, 식욕을 증진시키는 역할을 한다. 익은 김치는 유기산,알코올,에스텔을 생산하여 식욕을 증진시키며, 김치가 익을 때 나오는 젖산균은 해로운 세균의 작용을 억제하며 새콤한 맛을 낼 뿐 아니라 창자속의 다른 균을 억제하여 이상 발효를 막아주고, 병원균을 억제한다. 김치의 효능은 항균활성, 정장작용, 항돌연변이, 암예방 및 항암효과, 항동맥경화, 면역력 증강 및 비만억제효과 등이 알려져 있다. 그러나 김치는 숙성과정에서 유산균의 증식으로 상큼하고 신맛이 좋아지는 반면에 시간이 경과하게 되면 잡균이 생육하게 되어 김치가 물러져 연부현상이 나타나 김치의 식감과 저장성을 떨어 뜨리게 된다. Kimchi picks radishes, cabbages and cucumbers into salt and mixes spices such as red pepper, garlic, parsley, ginger, salted fish, sugar and salt, and then puts in kimchi. Kimchi is fermented during fermentation, lactic acid bacteria and lactic acid to produce various organic acids, so fresh and sour, delicious kimchi is made. Kimchi is a fermented food, rich in various minerals and vitamins, rich in nutrients, low in calories, and high in fiber. It is known that capsaicin, which is the main component of red pepper in kimchi, It is known as a functional food. Salted seafood is decomposed into amino acids and becomes a protein source. Vegetables are rich in minerals such as calcium, copper, phosphorus, iron, and salt, which helps to absorb vitamin B. Kimchi 's vegetables cleans the intestines by the combined action of juice and salt, promotes the secretion of protein, the protease in the stomach, and normalizes intestinal microbial distribution. Fibers also prevent constipation and prevent diseases such as enteritis and colonitis. Lactic acid bacteria by the action to help the digestion, and improve appetite role. Rice Kimchi produces organic acids, alcohol, and estel to improve appetite. Lactic acid bacteria that come out when kimchi is ripe inhibit the action of harmful bacteria and not only produce a sour taste, but also inhibit abnormal fermentation by inhibiting other bacteria in the intestines. . The efficacy of Kimchi is known to be antibacterial activity, suicide action, antimutagenic effect, cancer prevention and anti - cancer effect, anti - arteriosclerosis, immunity enhancement and obesity inhibitory effect. However, when the kimchi is fermented during the fermentation process, the fermentation of lactic acid bacteria will produce fresh and sour taste, but when the time passes, the bacteria will grow and the kimchi will disappear and the softness of kimchi will deteriorate the texture and storage stability.
황태(黃太)육수는 황태를 끓여서 얻는 육수로서, 메치오닌, 트립토판, 라이신이 많이 들어 있어 인체의 세포 발육과, 피로회복, 혈압조절에도 효과가 있다. 또한 비타민A가 많이 들어 있어 눈에 좋다. Hwangtae (黃 太) Hwangsu is a boiled seaweed, which is rich in methionine, tryptophan and lysine. It has effects on cell development, fatigue and blood pressure. It also contains a lot of vitamin A and is good for eyes.
황태는 알래스카 등지에서 어획한 명태를 겨울철에 일교차가 큰 강원도 산악지역의 덕장에 걸어 차가운 바람을 맞으며 얼고 녹기를 20회 이상 반복해서 말린 북어로서, 자연의 에너지를 이용한 동결 건조된 명태를 말한다. 황태는 동결과 해동을 반복하면서 건조되어 명태의 단백질이 분해되어 아미노산이 많이 함유되어 있고, 색깔이 약간 누렇게 되고 육질이 연하고 부드러우면서 쫄깃한 깊은 맛이 난다. 황태는 숙취해소와 간장해독, 노폐물제거 등의 효능을 갖고 있으며, 무침, 구이, 찜, 국, 찌개 등으로 요리하여 이용된다. 강원도 산간지역의 황태덕장에서 건조하는 황태들은 밖에 거치하는 즉시 얼기 때문에 단백질의 부패가 없으므로 생선 비린내가 나지 않는다. 황태의 영양적인가치는 황태 가수분해물로부터 활성 peptide의 분리정제와 기능특성에 관한 연구 (조산순 박사학위논문, 공주대학교, 2008년)에 의하면, 황태 가수분해물로부터 활성 peptide를 분리정제하고 그 기능적 특성들을 측정한 결과 일반성분 분석에서 수분 함량은 12.5±0.2%, 단백질 함량은 43.3±0.2%, 조지방 함량은 0.4±0.1% 그리고 조회분 함량은 3.8±0.3 %였다. 아미노산 분석 결과 황태는 전체 아미노산 구성 중 free amino acid 구성 비율이 생태의 경우보다 24배 증가하였으며 황태의 PER 값은 생태에 비하여 약 1.6배 증가하였다. 황태에서의 불포화 지방산의 비율은 58%로 생태 43% 보다 증가하였으며 DHA 등의 오메가 불포화지방산이 함유되어 있었다.Dae-tae is a frozen-dried pollinate that uses nature's energy to dry polished rice roasted in Alaska and other places over 20 times in cold weather with cold winds by walking in the valley of Gangwon-do mountainous region in winter. It is dried with repeated freezing and thawing, and the protein of the pollack is decomposed to contain a lot of amino acids. The color is slightly yellowish, and the meat is soft, soft and has a deep flavor. It has the effect of removing hangover, detoxifying soy sauce, removing wastes, etc. It is used by cooking with grilled, steamed, steamed soup, steamed soup. In the mountains of Gangwon-do, the dried fish are free from frost as they are frozen immediately after they are placed outside, so there is no protein spoilage. According to the study on the separation and purification of the active peptide from the hydrolyzate of Prunus thermophilus, the active peptide was isolated and purified from the hydrolyzate of Prunus mume The moisture content, protein content and crude fat content were 12.5 ± 0.2%, 43.3 ± 0.2%, 0.4 ± 0.1% and 3.8 ± 0.3%, respectively. As a result of amino acid analysis, the free amino acid composition ratio of the total amino acid composition of Hwangtae was 24 times higher than that of the ecological case. The proportion of unsaturated fatty acids in the leaves was 58%, which was higher than that of 43% in the eco - system, and contained omega - unsaturated fatty acids such as DHA.
비타민C(Vitamin C)는 아스콜빈산으로 불리며, 강한 환원제로서 콜라겐의 합성 효소 활성화 등 인체에 있어 필수적인 성분 중 하나로서, 인체에서 비타민C가 결핍되면 괴혈병이 발생한다. 따라서 사람의 체내에서는 자체적으로 비타민C를 만들 수 없으므로 반드시 채소나 과일을 통해서 섭취해야만 한다. 비타민 C는 수용성의 항산화 물질로 신체를 활성산소로부터 보호하여 암, 동맥경화, 류머티즘 등을 예방해 주며, 면역 체계도 강화시킨다. 결합조직과 지지조직의 형성에 가담하여 피부와 잇몸의 건강을 지켜준다. 즉 비타민 C가 세포의 산화를 방지하므로 암과 각종 만성 질환을 예방 치료하고, 노화도 억제한다는 논문들이 발표됐다. 그러나 비타민C를 많이 함유한 부재료를 김치양념 조성물로 사용할 때는 유산균의 배지가 되므로 영양분과 수분의 조화를 잘 맞추어야 하며, 또한 유산균이 생성하는 유기산이 김치맛을 좌우하므로 엄선하여 사용해야 한다. Vitamin C , called as ascorbic acid, is a strong reducing agent and is one of the essential components of the human body, such as the activation of collagen synthesis enzymes. When vitamin C is deficient in the human body, scurvy occurs. Therefore, it can not make vitamin C in the human body itself, so it must be consumed through vegetables or fruits. Vitamin C is a water-soluble antioxidant that protects the body against active oxygen to prevent cancer, arteriosclerosis, rheumatism, and strengthens the immune system. It participates in the formation of connective tissue and supportive tissue to protect the health of skin and gums. In other words, Vitamin C prevents oxidation of cells, preventing and curing cancer and various chronic diseases, and suppressing senescence. However, when a sub ingredient containing a large amount of vitamin C is used as a kimchi seasoning composition, the nutrients and water must be harmonized because it becomes a medium for lactic acid bacteria. Also, the organic acid produced by lactic acid bacteria influences the taste of kimchi.
청국장은 주로 볏짚에서 생육하는 고초균(Bacillus subtilis)을 이용하여 삶은 콩을 실내에서 띄워 발효과정 중에 고초균이 생산하는 효소에 의해서 콩의 전분질과 단백질에서 유래된 점질물의 당류, 아미노산, 고분자 핵산, 갈변물질, 단백질 분해효소(혈전용해제), 폴리글루타메이트 등의 혼합물질을 생성하면서 독특한 맛과 냄새를 낸다. Chungkukjang mainly uses rice bacillus (Bacillus subtilis) grown in rice straw to boil the beans in the indoor space. During the fermentation process, the enzymes produced by Bacillus subtilis cause fermentation of sugars, amino acids, macromolecule nucleic acids and browning substances , Proteolytic enzymes (thrombolytic agents), and polyglutamates while producing a unique taste and smell.
청국장은 칼슘과 비타민 A·B의 중요한 공급원으로서 대두의 섬유질이 청국장 발효 균주와 섬유질의 복합적인 정장효과에 의해 장을 건강하게 할 수 있으며, 콩에서 기인된 이소플라본, 피트산, 사포닌, 트립신 저해제, 토코페롤, 불포화지방산, 식이섬유, 올리고당, γ-글루타밀트랜스펩티다제 등의 각종 생리활성물질, 항산화물질을 다량 함유하고 있는 기능성 식품이다. 또한 혈전용해 효소 및 항암물질이 풍부하여 암 예방효과가 있다. 청국장의 소화흡수율이 높다. Chungkukjang is an important source of calcium and vitamin A · B. Soybean fiber can make the intestines healthier by the complex effect of fermentation of Chongkukjang fermentation strain and fiber, and isoflavones, peptic acid, saponin, trypsin inhibitor , Tocopherol, unsaturated fatty acids, dietary fiber, oligosaccharide,? -Glutamyltranspeptidase and the like, and antioxidant substances. In addition, there is abundant thrombolytic enzyme and anticancer substance, and it has cancer prevention effect. The digestion and absorption rate of Cheonggukjang is high.
청각(sea staghorn, 靑角)은 바닷속에 사는 해조류로서 사슴뿔 모양으로 자라며, 비타민C가 풍부하게 함유되어 있고, 칼슘과 철분이 다량 함유되어 골다공증 예방과 빈혈 예방에도 좋다. 고추의 캡사이신과 더불어 김치의 신맛을 방지하면서 아삭한 김치맛을 유지해 준다. 끓는 물에 청각을 살짝 데치고 찬물로 헹궈주면 싱싱한 녹색빛이 난다. 적당한 크기로 절단하여 김치속의 조성물로 사용하여 김치의 맛을 내는데 이용된다. 김치를 담글 때 넣으면 젓갈이나 생선의 비린내, 마늘 냄새를 중화시켜 뒷맛을 개운하게 한다. 구충 성분이 있어 예전에는 회충약으로 쓰이기도 하였으며, 수용성 추출물은 세균에 대해 강한 항생 작용을 가지고 있다고 알려져 있다 The sea staghorn ( sea staghorn) is a seaweed living in the sea. It grows in the shape of a deer horn. It is rich in vitamin C, and it contains calcium and iron in large amounts, so it is good for prevention of osteoporosis and prevention of anemia. In addition to capsaicin of pepper, it keeps the taste of crispy kimchi while preventing the sourness of kimchi. Rinse your hearing in boiling water and rinse with cold water to get a fresh green light. It is used to cut Kimchi into a suitable size and use it as a Kimchi composition to taste Kimchi. When you put Kimchi in soaking, you can neutralize the smell of salted fish, fish smell, and garlic to make your aftertaste fresh. It has also been used as a pesticide in the past because of its antiparasitic properties, and its water-soluble extract is known to have strong antibiotic action against bacteria
청각은 혈액내 독소를 제거하고 재생세포를 활성화하는 효능이 알려져 있다. 무기질과 비타민이 풍부한 알카리성 식품으로 칼슘과 인이 풍부하여 어린이의 뼈발육에 좋고, 철분이 많아 빈혈예방에도 좋다. 또한 항암 성분의 베타카로틴을 함유하고 있어 면역력과 암세포 성장을 억제하는 효능이 있다. Hearing is known to be effective in removing blood toxins and activating regenerative cells. Alkaline food rich in minerals and vitamins, rich in calcium and phosphorus, good for the development of children's bones, iron is good for the prevention of anemia. It also contains anti-cancer beta-carotene, which has the potential to inhibit immune system and cancer cell growth.
녹차Green Tea , 綠茶 ) 분말은 녹차 잎을 즉시 가열하여 산화효소를 파괴시켜 녹색을 유지시키는 동시에, 수분을 증발시켜 잎을 어느 정도 바삭바삭하게 만든다. 녹차에 단맛과 상쾌한 맛을 전해주는 데아닌(theanine)은 머리를 맑게 해주는 작용도 해준다. 녹차의 함유물 가운데 데아닌이라는 물질이 별다른 부작용 없이 알파(α)파를 유의하게 증가시킨다는 사실을 확인했음을 밝혔다. 섭취 후 약 30분 이내에 뇌에 도달하며, 40분 정도면 뇌에서 알파파를 생성했다. 알파파는 암기력 향상과 스트레스 회복 촉진 등에 영향을 미치는 것으로 알려져 있으며, 숙면을 취하는 데에도 도움을 준다. 이러한 효과를 내는 데아닌은 차에만 함유되어 있는 고유한 성분으로, 현미 등의 성분을 첨가한 혼합 녹차보다는 100% 순수 녹차를 마실 때 더 큰 효과를 기대할 수 있다. 녹차 중의 폴리페놀류는 항암작용, 혈중 콜레스테롤 저하작용, 혈관신생억제 작용 및 혈당 저하 효과 등이 있는 것으로 알려져 있다. 또한 녹차는 열량이 지닌 성분이 거의 없고 지방 축적 억제작용이 있어서 다이어트 효과가 있다. Green tea Tea, 綠茶 ) powder instantly heats the green tea leaves to destroy the oxidizing enzyme to keep the green, while at the same time evaporating the water makes the leaves crispy to some extent. Theanine, which imparts sweetness and refreshing taste to green tea, also helps to clear the hair. And that the substance called denier in the green tea content significantly increased the alpha (alpha) wave without any side effects. Approximately 30 minutes after ingestion, reaching the brain, and about 40 minutes, the brain produced alfa. Alpha waves are known to affect the improvement of memory and stress recovery, and also help to take a good night's sleep. This effect is a unique ingredient contained only in the tea. It can be expected to have a greater effect when drinking 100% pure green tea than mixed green tea with ingredients such as brown rice. It is known that polyphenols in green tea have anticancer effect, blood cholesterol lowering effect, angiogenesis inhibitory action and hypoglycemic effect. Green tea has little calorie content and has a fat-suppressing action, so it has a diet effect.
고춧가루(hot pepper powder)는 비타민 A·B·C가 풍부한 데, 그 중에서 비타민 C는 감귤의 9배, 사과의 18배나 될 정도로 매우 풍부하다. 고추의 매운맛은 캡사이신(Capsaicine)과 디히드로캡사이신(dihydrocapsaicin)이라는 물질로서 품종과 재배환경에 따라 큰 차이가 난다. 고춧가루는 김치 담기나, 고추장 제조에 쓰이고, 각종 요리류의 조미료 등으로 사용한다. Hot pepper powder is rich in vitamins A, B, and C, among which vitamin C is very abundant, nine times as high as citrus fruits and 18 times as high as apples. The spicy taste of red pepper is a substance called capsaicine (dihydrocapsaicin) and dihydrocapsaicin. Red pepper powder is used for making kimchi and for making red pepper paste, and it is used for seasoning of various kinds of dishes.
캡사이신은 소화액 분비를 자극하여 소화기능을 촉진하며, 고춧가루는 매운맛을 내면서도 단맛이 나고, 색소는 강렬하면서 비타민 C함량이 높으며, 젓갈, 소금 등과 어우러져 효소를 생성하여 몸의 지방질을 분해하는 역할을 하는 것에 특징이 있다. 고추의 색깔은 50종 이상의 카로테노이드로 구성되어 있으며 캅산틴(0.41%, 색소의 35%), 캅소루빈, β-카로텐(0.06%, 색소의 12%) 등이 주요 색소이다. 고추는 감기나 기관지염, 가래 제거에 효과가 있으며 더불어 지방을 분해하는 기능이 밝혀져 비만증을 방지할 수 있다는 연구결과도 있다.Capsaicin promotes the digestive function by stimulating digestive juice secretion. Red pepper powder has a sweet taste while spicy, has a strong coloring, high vitamin C content, and is formed with enzymes such as seafood, salt, etc. to decompose body fat . The color of red pepper is composed of more than 50 kinds of carotenoids. Major coloring matters are capsanthin (0.41%, 35% of pigment), capsorubin, β - carotene (0.06%, 12% of pigment). Studies have shown that red pepper is effective in removing colds, bronchitis, and sputum, and it also has the ability to break down fat to prevent obesity.
김치는 숙성과정에서 유산균이 증식되면서 1-2주일 정도 경과시에 상큼하고 맛좋은 신맛이 최고조에 달하는 반면에, 온도가 상승하거나 숙성시간이 경과하게 되면 배추김치가 물러져 연부현상이 나타나 김치의 식감과 저장성을 떨어 뜨리게 된다. 이러한 원인은 김치 속에 유산균의 먹이가 되는 배지 중에 잔류하는 설탕 등의 탄수화물 농도가 높거나, 배추의 섬유질을 이루고 있는 팩틴(Pectin)과 셀루로스(Cellulose)가 팩틴분해효소와 섬유질분해효소의 작용을 받아 김치 고유의 식감인 조직감과 씹힘성이 떨어지게 된다. Kimchi has a fresh and tasty sour taste when the lactic acid bacteria are proliferated during the fermentation process, but when the temperature rises or the aging time elapses, the Chinese cabbage kimchi is withdrawn, The storage stability is lowered. The reason for this is that the concentration of carbohydrates such as sugar remaining in the medium that feeds on lactic acid bacteria is high in kimchi, or that pectin and cellulose, which form the fibers of Chinese cabbage, have the action of pectinase and fibrotic enzyme And the texture and chewiness of Kimchi are reduced.
또한 김치의 맛을 좋게 하기 위하여 사용하는 설탕과 MSG로 인하여 유산균과 잡균의 증식이 빨라지게 되어 김치가 쉽게 시어지고 맛이 나빠지게 된다. 또한 건강을 추구하는 소비자로부터 설탕과 MSG를 사용하지 않는 김치를 구입하려는 추세로 바뀌고 있다.Also, because of the sugar and MSG used to improve the taste of kimchi, the proliferation of lactic acid bacteria and germs is accelerated and the kimchi is easily sour and the taste is deteriorated. It is also turning into a trend to buy kimchi that does not use sugar and MSG from health-conscious consumers.
본 발명은 이러한 문제점을 해결하기 위하여 연구를 거듭한 끝에 김치양념으로 사용하는 당류나 전분질의 사용량을 유산균이 증식에 필요로 하는 최소량만을 사용하고, 대신에 기능성당을 사용한다. 그리고 청국장을 첨가하여 바실러스균으로 하여금 당을 소비하도록 하여 유산균의 증식을 억제시키도록 하는데 있다. 또한, 종래에 배추의 팩틴과 본 발명은 이러한 문제점을 해결하기 위하여 연구를 거듭한 끝에 김치양념으로 사용하는 당류나 전분질의 사용량을 유산균이 증식에 필요로 하는 최소량만을 사용하고, 대신에 기능성당을 사용한다. 그리고 청국장을 첨가하여 바실러스균으로 하여금 당을 소비하도록 하여 유산균의 증식을 억제시키도록 하는데 있다. 또한, 종래에 배추의 팩틴과 섬유질 분해효소의 생육을 억제시키기 위하여 글리신 또는 알라닌 등의 아미노산을 첨가하는 대신에, 황태육수를 사용하므로써 팩틴과 섬유질분해효소의 활성을 억제시켜 김치의 조직감과 씹힘성 및 아미노산이 첨가되어 더욱 맛있는 김치를 제공할 수 있으므로 상미기간을 연장시킬 수 있다. 황태육수는 냉동과 건조 과정을 반복하면서 단백질이 각종 아미노산으로 분해되어 있으며, 값싸고 손쉽게 만들어 사용할 수 있다. 더욱이 종래 김치담기에 사용하던 수분 대신에 황태육수를 사용하므로서 아미노산이 첨가되어 더욱 맛있는 김치를 제공할 수 있다. In order to overcome such problems, the present invention uses only a minimum amount of saccharide or starch to be used in kimchi sauce for the proliferation of lactic acid bacteria, and uses a functional sugar instead. In addition, the addition of Chungkukjang to consume the sugar bacillus bacteria to inhibit the growth of lactic acid bacteria. Conventionally, pectin of Chinese cabbage and the present invention have been studied in order to overcome such a problem, and it has been found that the use amount of saccharides or starches used in kimchi sauce is used only in the minimum amount required for lactic acid bacteria to multiply, use. In addition, the addition of Chungkukjang to consume the sugar bacillus bacteria to inhibit the growth of lactic acid bacteria. In addition, in order to suppress the growth of pectin and fibrotic enzymes of Chinese cabbage, instead of adding amino acids such as glycine or alanine, the activity of pectin and fibrolytic enzymes is inhibited by using potato broth, and the texture and chewiness Amino acids are added to provide more delicious kimchi, so you can prolong the steak period. Protein is decomposed into various amino acids by repeating the freezing and drying process, and it is cheap and easy to use. Furthermore, in place of the water used in the conventional kimchi addition, amino acid is added by using the fresh spring water to provide more delicious kimchi.
본 발명은 황태 육수와 청국장에 비타민C, 청각, 녹차분말, 및 고춧가루를 섞어 비타민C를 강화시킨 김치조성물을 만든 다음, 숙성시키는 단계와, 새우젓, 멸치액젖, 마늘, 생강, 양파, 설탕 및 상기의 비타민C를 강화시킨 김치조성물을 혼합하여 김치양념을 만드는 단계와, 절인배추에 상기의 비타민C를 강화시킨 김치조성물이 혼합된 김치양념으로 기능성김치 담기하는 단계와, 상기의 기능성김치를 숙성 및 발효하는 단계로 구성되는 비타민C를 강화시킨 김치조성물 및 이를 이용한 본 발명은 비타민C를 강화시킨 김치조성물 및 이를 이용한 항산화활성 및 미백효과의 기능성김치의 제조방법에 관한 것이다.The present invention relates to a method of preparing a kimchi composition comprising vitamin C, hearing, green tea powder, and red pepper powder mixed with the Hwangtae juice and chungkukjang to enhance vitamin C, Preparing a kimchi sauce by mixing the kimchi composition with vitamin C, and adding the functional kimchi to the pickled Chinese cabbage in a kimchi sauce mixed with the above-mentioned vitamin C-enhanced kimchi composition; Fermenting vitamin C, and a method of manufacturing a functional kimchi having antioxidant activity and whitening effect using the composition.
본 발명의 황태육수를 포함한 김치조성물로 만든 김치는 일반김치에 비교하여 총 아미노산이 3주일간 숙성시 152%, 6주일간 숙성시 156% 증가한다. 특히 맛에 관여하는 글루타민산과 글루타민이 증가하므로 MSG를 첨가하지 않더라도 맛있는 김치를 제공할 수 있다. 또한 알라닌 또는 글리신 등의 함량이 높아 배추의 팩틴분해효소와 섬유질분해효소의 활성을 억제시킬 수 있으므로 김치의 조직감과 씹힘성이 우수한 맛있는 김치를 제공할 수 있다.The kimchi composition of the present invention containing kimchi composition containing Hwangyu soup water has a total amino acid content of 152% when fermented for 3 weeks and 156% when fermented for 6 weeks. Especially, since glutamic acid and glutamine which are involved in the taste are increased, delicious kimchi can be provided without addition of MSG. Also, since the content of alanine or glycine is high, the activity of picinase and fibrolytic enzyme of Chinese cabbage can be inhibited, so that a delicious kimchi having excellent texture and chewiness of kimchi can be provided.
본 발명은 항산화활성 및 미백효과의 기능성을 제공할 수 있다.The present invention can provide antioxidant activity and functionality of whitening effect.
종래에 배추의 팩틴과 섬유질 분해효소의 생육을 억제시키기 위하여 글리신 또는 알라닌 등의 아미노산을 첨가하는 대신에, 황태육수를 사용하므로써 팩틴과 섬유질분해효소의 활성을 억제시켜 김치의 조직감과 씹힘성 및 아미노산이 첨가되어 더욱 맛있는 김치를 제공할 수 있으므로 상미기간을 연장시킬 수 있다.Conventionally, instead of adding amino acids such as glycine or alanine in order to inhibit the growth of pacific and fibrotic enzymes of Chinese cabbage, the activity of pectin and fibrotic enzymes is inhibited by using the radish juice, so that the texture and chewiness of kimchi, It is possible to add more delicious kimchi and thus to prolong the freshness period.
본 발명의 황태육수를 포함한 김치조성물로 만든 김치는 일반김치에 비교하여 총 아미노산이 3주일간 숙성시 152%, 6주일간 숙성시 156% 증가한다. 특히 맛에 관여하는 글루타민산과 글루타민이 증가하므로 MSG를 첨가하지 않더라도 맛있는 김치를 제공할 수 있다. 또한 알라닌 또는 글리신 등의 함량이 높아 배추의 팩틴분해효소와 섬유질분해효소의 활성을 억제시킬 수 있으므로 김치의 조직감과 씹힘성이 우수한 맛있는 김치를 제공할 수 있다.The kimchi composition of the present invention containing kimchi composition containing Hwangyu soup water has a total amino acid content of 152% when fermented for 3 weeks and 156% when fermented for 6 weeks. Especially, since glutamic acid and glutamine which are involved in the taste are increased, delicious kimchi can be provided without addition of MSG. Also, since the content of alanine or glycine is high, the activity of picinase and fibrolytic enzyme of Chinese cabbage can be inhibited, so that a delicious kimchi having excellent texture and chewiness of kimchi can be provided.
본 발명의 비타민C가 강화된 김치는 김치의 시어짐과 연부 현상을 방지할 수 있으며, 상큼하고 시원한 맛의 김치를 제공할 수 있다. 또한 본 발명은 항산화활성 및 미백효과의 기능성을 제공할 수 있다.The vitamin C-enriched kimchi of the present invention can prevent the sagging and the edge phenomenon of the kimchi and provide a fresh and cool taste of kimchi. The present invention can also provide antioxidant activity and whitening effect functionality.
도 1은 본 발명의 제조공정도이다.1 is a manufacturing process diagram of the present invention.
본 발명은 황태 육수와 청국장에 비타민C, 청각, 녹차분말, 및 고춧가루를 섞어 비타민C를 강화시킨 김치조성물을 만든 다음, 숙성시키는 단계와, 새우젓, 멸치액젖, 마늘, 생강, 양파, 설탕 및 상기의 비타민C 강화용 조성물을 혼합하여 김치양념을 만드는 단계와, 절인배추에 상기의 비타민C 강화용 조성물이 혼합된 김치양념으로 김치담기하는 단계와, 상기의 김치를 숙성 및 발효하는 단계로 구성되는 비타민C를 강화시킨 김치조성물 및 이를 이용한 김치의 제조방법에 관한 것이다.The present invention relates to a method of preparing a kimchi composition comprising vitamin C, hearing, green tea powder, and red pepper powder mixed with the Hwangtae juice and chungkukjang to enhance vitamin C, Preparing a kimchi sauce by mixing a vitamin C composition for enhancing vitamin C, adding kimchi to a pickled Chinese cabbage with a kimchi sauce mixed with the above vitamin C enhancing composition, and aging and fermenting the kimchi A vitamin C-enriched kimchi composition, and a method for producing a kimchi using the same.
<제조예 1>; 비타민C를 강화시킨 김치조성물을 이용한 기능성김치 담기≪ Preparation Example 1 > Add functional kimchi with vitamin C-enhanced kimchi composition
1) 황태 육수1)
황태 육수는 단백질이 분해되어 메치오닌, 트립토판, 라이신이 많이 들어 있어 시원하고 개운한 맛을 주는 역할을 하고, 인체의 세포 발육과 피로회복, 혈압조절에도 효과가 있다. 또한 비타민A가 많이 들어 있어 눈에 좋다. 황태덕장(인제군 용대리)에서 동결건조하여 말린 황태를 500g을 구입(가락동시장)하여 황태 1마리 또는 황태 머리 5 개에 정제수 1리터를 붓고 100℃ 이상에서 0.5∼2시간 동안 황태살이 흐물해질 때 까지 끓이고 고아서 0.5리터가 되도록 조림한다. 황태 조림을 으깨고 분쇄하여 거즈나 사랑천에 걸러서 황태 육수 0.5ℓ를 얻는다. 절인배추 2kg에 대하여 황태육수 0.25ℓ를 사용한다. Hwang Tae Sung water is a protein decomposition that contains a lot of methionine, tryptophan and lysine, it plays a role of giving a cool and refreshing taste, and is also effective in regulating the cell development, fatigue and blood pressure of the human body. It also contains a lot of vitamin A and is good for eyes. It is freeze-dried in Han-Taegyok-dong (Inje-gun, Yongda-ri) and purchased 500g of Dried Chrysanthemum (Kang-dong market), and 1 liter of purified water is poured into 1 chrysanthemum or 5 heads of Chrysanthemum and it is heated to 100 ℃ or more for 0.5 ~ 2 hours Boil, boil and simmer to 0.5 liter. It crushes and crushes the dwarf simmered bean, and it is filtered by gauze or love cloth to obtain 0.5 liters of Hwang Tae broth. For 2kg of marinated Chinese cabbage, 0.25ℓ of Hwang Tae broth is used.
2) 고춧가루2) red pepper powder
고춧가루는 비타민C가 풍부하게 함유되어 있고, 캡사이신의 매운맛과 어우러져 맵고 시원하면서 단맛을 내는 상승효과를 제공해 준다. 국내산으로 괴산, 음성, 영양, 청양 등지에서 수확한 맵고 단맛이 나는 태양초 햇고추가루(괴산농협)를 구입하여 사용한다. 절인배추 2kg에 대하여 고춧가루 120∼140중량부를 사용한다. 고춧가루의 입도분포가 35~45mesh이고, 수분함량 12~13%의 고춧가루를 사용한다. The red pepper powder is rich in vitamin C, and it is combined with the spicy taste of capsaicin, giving it a spicy, cool, sweetening synergy. Purchase and use the sweet and sour chili powder (Goesan Nonghyup) harvested from Goesan, Voice, Nutrition and Cheongyang in Korea. 120 to 140 parts by weight of red pepper powder is used for 2 kg of pickled cabbage. Red pepper powder with a particle size distribution of 35 ~ 45 mesh and a water content of 12 ~ 13% is used.
3) 비타민C 3) Vitamin C
비타민C는 인체에서 항바이러스제이고 면역력을 높여 주며, 혈관을 깨끗하게 만들고 면역력을 높인다고 알려져 있다. 또한 피부의 기저막을 형성해 피부를 좋게 하는 효과도 있다. 비타민C는 아스콜빈산을 구입(신원무역상사) 절인배추 2kg에 대하여 4∼6중량부의 아스콜빈산을 사용한다.Vitamin C is an antiviral agent in the body, enhances immunity, cleanses blood vessels and improves immunity. It also has the effect of improving the skin by forming a basement membrane of the skin. Vitamin C uses 4 to 6 parts by weight of ascorbic acid per 2 kg of pickled cabbage (purchased from Shin Won Trading Co.) for purchasing ascorbic acid.
4) 청국장4) Cheonggukjang
비타민C가 풍부하게 함유되어 있는 청국장을 구입(청국장 순창김옥희할머니)하여 절인배추 2kg에 대하여 청국장 40∼60중량부를 사용한다. 본 발명의 김치조성물에서 청국장은 미세한 분말형태로 김치에 첨가되는 것이 바람직하므로 50∼100mesh 크기의 입자를 청국장을 사용하는 것이 김치 속에 고르게 분산시킬 수 있다.Purchase of Chungkukjang, which is rich in vitamin C (Chungkukjang, Sunchang, Kim, Ok-hee), 40 to 60 parts by weight of chungkukjang are used for 2kg of pickled cabbage. In the kimchi composition of the present invention, it is preferable that the chungkukjang be added to the kimchi in the form of fine powder, so that the particles having a size of 50 to 100mesh can be evenly dispersed in the kimchi using the chungkukjang.
5) 청각5) Hearing
청각은 바닷속에 사는 해조류로서 비타민C가 풍부하게 함유되어 있고, 칼슘과 철분이 다량 함유되어 골다공증 예방과 빈혈 예방에도 좋다. 고추의 캡사이신과 더불어 김치의 신맛을 방지하면서 아삭한 김치맛을 유지해 준다. 청각은 완도산을 구입하여 500g을 구입하여 100℃ 끓는 물에 청각을 살짝 데치고 찬물로 헹궈주면 싱싱한 녹색빛이 난다. 5mm 크기로 절단한 청각을 절인배추 2kg에 대하여 8∼12중량부를 사용한다.Hearing is a seaweed living in the sea, rich in vitamin C, and contains a large amount of calcium and iron to prevent osteoporosis and prevent anemia. In addition to capsaicin of pepper, it keeps the taste of crispy kimchi while preventing the sourness of kimchi. Purchase 500g for the hearing, buy Wando, pick up the hearing in 100 ℃ boiling water and rinse with cold water. 8 to 12 parts by weight of hearing aid cut into a size of 5 mm is used for 2 kg of pickled Chinese cabbage.
6) 녹차 분말6) Green tea powder
녹차는 보성녹차 100g을 구입하여 비타민C가 풍부하게 함유되어 있으며, 녹차 중의 폴리페놀류는 항암작용, 혈중 콜레스테롤 저하작용, 혈관신생억제 작용 및 혈당 저하 효과 등이 있는 것으로 알려져 있다. 또한 녹차는 열량이 지닌 성분이 거의 없고 지방 축적 억제작용이 있어서 다이어트 효과가 있다. 절인배추 2kg에 대하여 녹차 분말 4∼6중량부를 사용한다. The green tea is rich in vitamin C by purchasing 100 g of Boseong green tea. It is known that polyphenols in green tea have anticancer effect, blood cholesterol lowering effect, angiogenesis inhibitory action and hypoglycemic effect. Green tea has little calorie content and has a fat-suppressing action, so it has a diet effect. 4 to 6 parts by weight of green tea powder is used relative to 2 kg of pickled cabbage.
그 밖의 비타민C를 많이 함유한 소재를 조성물에 포함시킬 수도 있으나, 김치속 조성물로 사용했을 때, 맛과 수분의 조화를 위하여 엄선하여 사용한다.Other vitamin C-containing materials may be included in the composition, but when used as a kimchi composition, they are carefully selected for harmony of taste and moisture.
영양분과 수분은 유산균의 배지로서 김치맛을 좌우하는 중요한 요소이다.Nutrients and moisture are important factors that influence the taste of kimchi as a medium for lactic acid bacteria.
7) 김치조성물의 혼합 및 숙성7) Mixing and Aging of Kimchi Composition
절인배추 2kg에 대하여 상기의 6가지 원부재료인 고춧가루 120∼140중량부, 비타민C 4∼6중량부, 청국장 40∼60중량부, 청각 8∼12중량부, 녹차분말 4∼6중량부, 황태 육수 0.4∼0.6리터를 섞어 이들 조성물을 반죽한 다음, 3∼5℃에서 8∼11일간 숙성시킨다. 2 kg of the pickled cabbage were mixed with 120-140 parts by weight of red pepper powder, 4-6 parts by weight of vitamin C, 40-60 parts by weight of chungkukjang, 8-12 parts by weight of hearing, 4-6 parts by weight of green tea powder, 0.4 to 0.6 liters of broth are kneaded, and these compositions are kneaded and aged at 3-5 DEG C for 8 to 11 days.
8) 찹쌀풀8) glutinous rice paste
찹쌀 풀은 양념 혼합물에 첨가되는 채소류의 풋내와 젓갈류의 냄새를 없애주고 숙성이 되면 양념의 점성을 향상시키는 역할을 한다. 절인배추 2kg에 대하여 찹쌀풀 만들기는 찹쌀가루 40∼60중량부에 황태 육수 1리터를 용기에 넣고, 40∼50℃로 가열하면서 바닥이 ??지 않도록 휘저어 찹쌀 풀을 만든다. The glutinous rice paste removes the smell of salted and salted fishes added to the seasoning mixture and improves the viscosity of the seasoning when it is aged. About 2kg of marinated Chinese cabbage, glutinous rice paste is prepared by adding 1 liter of Hwangseo broth to 40 ~ 60 parts of glutinous rice flour and stirring at 40 ~ 50 ℃ to make glutinous rice paste.
김치 양념은 절인 배추에 섞이게 되면 배추 고유의 맛과 양념의 다양한 맛이 조화되어 김치의 맛과 품질 및 선호도를 향상시키는 역할을 하게 된다.When kimchi seasoning is mixed with pickled cabbage, it tries to harmonize the flavor of original cabbage and various flavors of seasoning to improve taste, quality and preference of kimchi.
9) 새우젓은 소화 효소뿐만 아니라 비타민 B1, 나이아신 등의 영양분이 풍부하여 구내염, 신경증 등을 예방하면서 감칠맛을 내는 역할을 하며 절인배추 2kg에 대하여 70∼90중량부를 사용한다. 9) Salted shrimps are rich in nutrients such as vitamin B1, niacin, etc., as well as digestive enzymes, so as to prevent the stomatitis and neurosis, and to give a rich taste. 70 to 90 parts by weight of 2 kg of pickled cabbage is used.
10) 멸치액젖은 젓갈원료의 육내에 존재하는 단백질분해효소와 미생물의 활성에 의한 자가소화로 인하여 고분자의 펩티드가 디펩티드, 트리펩티드 및 유리 아미노산으로 분해되어 양념혼합물에 사용시 특유의 맛을 내는 역할을 하며 절인배추 2kg에 대하여 50∼70중량부를 사용한다. 10) Anchovy Liquor is an enzyme that decomposes the peptides of peptides into dipeptides, tripeptides and free amino acids due to self-digestion of proteolytic enzymes and microorganisms present in the meat of the raw materials, And 50 to 70 parts by weight based on 2 kg of pickled Chinese cabbage.
11) 마늘은 김치의 발효를 촉진시키고, 살균 및 향균작용을 향상시키는 역할을 하며 절인배추 2kg에 대하여 40∼60중량부를 사용한다. 11) Garlic promotes the fermentation of kimchi and improves bactericidal and antibacterial activity, and 40-60 parts by weight of 2 kg of pickled Chinese cabbage is used.
12) 생강은 식욕을 돋워주면서 소화를 돕고 살균작용을 하는 것으로 양념혼합물에 어우러져 양념의 맛을 향상시키는 역할을 하며 절인배추 2kg에 대하여 8∼11중량부를 사용한다. 12) Ginger enhances appetite, helps digestion, and acts as a sterilizing agent. It improves the taste of seasoning by mixing with seasoning mixture. 8-111 parts by weight of 2kg of pickled cabbage is used.
13) 양파는 포도당, 과당, 맥아당이 함유되어 단맛을 내고, 비타민 B1,B2,C 등이 풍부하며, 소화액의 분비를 촉진하고 비타민 B1의 흡수를 도우며 신진대사를 활발하게 하며 절인배추 2kg에 대하여 40∼60중량부를 사용한다. 13) Onion is rich in glucose, fructose, and maltose, rich in vitamins B1, B2, and C. It promotes secretion of digestive juice, helps absorption of vitamin B1 and stimulates metabolism. 40 to 60 parts by weight are used.
14) 기능성당은 올리고당, 당알코올, 사이클로덱스트린 중에서 선택할 수 있다. 올리고당(oligosaccharides)은 말토올리고당, 이소말토올리고당, 프락토올리고당, 가락토올리고당 및 자일로올리고당 중에서 선택할 수 있다. 올리고당은 양념혼합물에 함유되는 각 성분들의 강한 향을 중화시키는 역할을 하며 절인배추 2kg에 대하여 35∼45중량부를 사용한다. 14) Functional sugar can be selected from oligosaccharides, sugar alcohols, and cyclodextrins. Oligosaccharides can be selected from maltooligosaccharides, isomaltooligosaccharides, fructooligosaccharides, maltooligosaccharides and xyloglucose. The oligosaccharide serves to neutralize the strong fragrance of the components contained in the seasoning mixture, and 35 to 45 parts by weight of 2 kg of pickled Chinese cabbage is used.
상기와 같이 비타민C가 강화된 김치조성물을 숙성시킨 후, 김치양념 7종과 혼합하여 김치속을 만든다. After the vitamin C-enriched kimchi composition is aged as described above, it is mixed with seven kinds of kimchi seasoning to make kimchi.
16) 김치담기16) Add Kimchi
절인배추 1포기(2kg)에 상기의 김치속을 켜켜이 집어넣고 버무리어 김치를 담구었다. 그 밖에 김치속 부속물은 철에 따라 갓, 부추, 대파, 쪽파, 무, 생새우 등을 사용할 수 있다.I put the above kimchi in a pickle (1kg) of pickled cabbage and put the kimchi in a batter. In addition, it is possible to use ginseng, leek, green onion, green onion, radish, raw shrimp and so on.
17) 숙성 및 저장17) Aging and storage
상기와 같이 담근 김치를 3∼5℃에서 1∼16주간 숙성시킨다. The kimchi thus dipped is aged at 3 to 5 DEG C for 1 to 16 weeks.
<실시예 1-6> ≪ Example 1-6 >
절임배추에 김치속의 사용량과 비타민C 강화용 조성물의 사용량에 따라 다음의 표 1과 같은 배합비로 구성하였다. 절인 배추 2kg에 김치속을 켜켜이 넣고 버무리어 김치를 제조한 후, 김치냉장고에 넣고 4℃에서 1∼6주일간 숙성시켰다.The composition of the pickled Chinese cabbage was as shown in Table 1 according to the amount of Kimchi used and the amount of vitamin C-containing composition used. Kimchi was added to 2kg of marinated Chinese cabbage, and the kimchi was put into a kimchi refrigerator and aged at 4 ℃ for 1 to 6 weeks.
강화
김치
조성물Vitamin C
reinforce
Kimchi
Composition
양념Kimchi
seasoning
(상기에서 IMOS는 이소말토올리고당이다.) (In the above, IMOS is isomaltooligosaccharide.)
상기의 실시예 1 내지 6에서 비타민C 강화 김치조성물 중의 황태육수는 100중량부를 동일하게 사용하고, 나머지 고춧가루, 비타민C, 청국장, 청각, 녹차가루는 숙성기간이 길수록 첨가량을 늘려서 사용하였다. 정성적인 관능검사를 통하여 실시예 3와 실시예 5에서 맛, 기호성, 조직감이 우수한 것으로 나타났다.In the above-mentioned Examples 1 to 6, 100 parts by weight of radish juice in the vitamin C-enriched kimchi composition was used in the same manner, and the remaining red pepper powder, vitamin C, chungkukjang, hearing aid and green tea powder were used in an increasing amount as the aging period was longer. The taste, palatability and texture were excellent in Examples 3 and 5 through qualitative sensory test.
<비교예>; 일반김치 담기≪ Comparative Example > Add a generic kimchi
배추 100중량부에 대하여 고춧가루 5중량부, 마늘 4중량부, 생강 4중량부, 양파 6중량부, 새우젓 5.5중량부, 멸치액젖 4중량부, 설탕 7중량부, 대파 5중량부, 부추 5중량부, 참깨 3중량부, 찹쌀풀 10중량부, 무우채 15중량부를 혼합하여 김치양념을 만들었다. 절인배추에 상기의 김치양념을 켜켜이 넣고 일반김치를 담구었다. 4℃에서 3주일간 숙성시켰다. 5 parts by weight of red pepper powder, 4 parts by weight of garlic, 4 parts by weight of ginger, 6 parts by weight of onion, 5.5 parts by weight of shrimp, 4 parts by weight of anchovy fluidized milk, 7 parts by weight of sugar, 3 parts by weight of sesame, 10 parts by weight of glutinous rice paste and 15 parts by weight of radish were mixed to prepare a kimchi sauce. I put the kimchi sauce on the pickled cabbage and dipped it in a regular kimchi. And aged at 4 캜 for 3 weeks.
비타민C 강화시킨 김치조성물과 김치양념으로 담근 김치와 일반김치의 이화학적인 특성 및 관능적인 특성을 알아 보기 위하여 다음의 시험예와 같이 실시하였다.In order to investigate the physicochemical and sensory characteristics of kimchi and kimchi dipped with vitamin C and kimchi sauce, the following test examples were conducted.
<시험예 1>; 이화학적 특성≪ Test Example 1 > Physicochemical properties
1) 아미노산 함량1) Amino acid content
본 발명은 배추의 팩틴과 섬유질 분해효소의 활성을 억제시키고자, 아미노산을 사용하는 대신에, 황태육수를 사용하여 이들 효소의 활성이 억제되어 아미노산이 함유된 맛있는 김치를 제공하며, 김치의 조직감과 씹힘성 및 상미기간을 연장시킬 수 있다. 본 발명의 김치(실시예6)와 일반김치를 5주일간 숙성시킨 후, 각각의 아미노산함량을 분석하여 다음의 표 2에 나타냈다.In order to inhibit the activity of pacific and fibrotic enzymes of Chinese cabbage, the present invention provides a delicious kimchi containing amino acids by inhibiting the activity of these enzymes by using potato broth instead of using amino acids, The chewiness and the shelf life can be prolonged. The kimchi of the present invention (Example 6) and the common kimchi were aged for 5 weeks, and their amino acid contents were analyzed and shown in Table 2 below.
아미노산 분석은 김치 10g을 동결건조시킨 후 이를 물로 추출하고, 0.45㎛ 멤브레인필터를 사용하여 여과한 후 에탄올/증류수/트리에틸아민/페닐이소시아네이트로 유도체화시켰다. 시료를 완전히 건조시킨 후 시험관에 넣고, 6N HCl 5㎖를 가하여 탈기한 후 밀봉하여 110℃에서 24시간 가수분해시킨 다음, 그 가수분해물을 증류수로 세척한 후, 건조시켜 염산을 제거한 다음 시료 희석용 완충액(0.2N sodium citrate buffer, pH2.2)으로 용해하였다. 이 용액을 여과한 후, 상기의 필터로 재차 여과하여 아미노산 자동분석기(Beckman system 6300, USA)로 다음과 같은 조건에서 분석하였다.(컬럼; Li 10cm column No.338051 ion exchange(Bekman), 이온교환수지; #2619, 분석시간; 150분, 완충액유속; 20㎖/hr, 닌하이드린 유속; 10㎖/hr, 컬럼압력; 1,380psi, 최적 샘플양; 50㎕, 질소가스압력; 40psi)For amino acid analysis, 10 g of kimchi was lyophilized, extracted with water, filtered using a 0.45 μm membrane filter, and then derivatized with ethanol / distilled water / triethylamine / phenyl isocyanate. The sample was thoroughly dried, placed in a test tube, degassed with 5N HCl 6N, sealed, and hydrolyzed at 110 ° C for 24 hours. The hydrolyzate was washed with distilled water, dried to remove hydrochloric acid, And dissolved in buffer (0.2 N sodium citrate buffer, pH 2.2). This solution was filtered and then filtered again with the above filter and analyzed with an amino acid automatic analyzer (Beckman system 6300, USA) under the following conditions (column: Li 10 cm column No. 338051 ion exchange (Bekman) 50 μl, Nitrogen gas pressure: 40 psi) at a flow rate of 20 ml / hr, a flow rate of ninhydrin of 10 ml / hr, a column pressure of 1,380 psi,
상기 표 2에서 나타난 바와 같이 황태육수김치는 일반김치에 비교하여 총 아미노산이 3주일간 숙성시 152%, 5주일간 숙성시 156%의 증가를 보였다. As shown in the above Table 2, the total amino acid content of Hwangtae seawater kimchi was 152% when fermented for 3 weeks and 156% when fermented for 5 weeks.
특히 맛에 관여하는 글루타민산과 글루타민이 증가하므로 MSG를 첨가하지 않더라도 맛있는 김치를 제공할 수 있다. 또한 알라닌 또는 글리신 등의 함량이 높아 배추의 팩틴분해효소와 섬유질분해효소의 활성을 억제시킬 수 있으므로 김치의 조직감과 씹힘성이 우수한 맛있는 김치를 제공할 수 있다.Especially, since glutamic acid and glutamine which are involved in the taste are increased, delicious kimchi can be provided without addition of MSG. Also, since the content of alanine or glycine is high, the activity of picinase and fibrolytic enzyme of Chinese cabbage can be inhibited, so that a delicious kimchi having excellent texture and chewiness of kimchi can be provided.
2) pH 및 총산도 2) pH and total acidity
본 발명의 김치와 일반김치 각각 10g을 증류수 50㎖를 가하여 분쇄한 후, 상기의 김치분쇄용액에 증류수를 보충하여 100㎖ 시료로 사용하였다. pH는 pH 메터기(pHM-210)를 이용하여 실온에서 측정하였다. 산도는 시료 10ml를 10배 희석하여 pH 8.3이 되도록 0.1N NaOH로 적정한 다음 젖산(%) 환원법으로 계산하였다. 10 g of each of the kimchi and common kimchi of the present invention was pulverized by adding 50 ml of distilled water, and the kimchi crushing solution was supplemented with distilled water and used as a 100 ml sample. The pH was measured at room temperature using a pH meter (pHM-210). The acidity was calculated by titrating 10 ml of the sample 10 times with 0.1 N NaOH to pH 8.3 and then by lactic acid (%) reduction method.
3) 미생물시험3) Microbiological test
본 발명의 김치와 일반김치를 담구어 숙성기간에 따라서 미생물의 총균수, 유산균수 및 효모곰팡이수를 식품공전의 미생물시험법(10-3-1)의 방법에 따라 측정하였다.The kimchi and general kimchi of the present invention were immersed and the total number of microorganisms, the number of lactic acid bacteria and the number of yeast fungi were measured according to the method of Microorganism Test Method (10-3-1) of Food and Drug Administration according to the aging period.
미생물 총균의 분석은 전체 생균계수(total counts), 효모곰팡이(yeast and mold) 및 유산균군(Leuconostoc 및 Lactobasillus)을 조사하였다. Total microbial counts, yeast and mold, and lactobacilli (Leuconostoc and Lactobasillus) were analyzed for microbial germs.
각각의 시료 10g과 살균한 0.1% 펩톤(peptone)수 90ml를 스토마커 백(stomacher bag)에 넣고 스토마커(stomacher; Lab blender, interscience, France)를 사용하여 2분 동안 균질화한 후, 펩톤수에 단계 희석하여 일반 세균은 PCA(plate count agar; DIFCO) 플레이트, 효모ㆍ곰팡이는 PDA(potato dextrose agar; DIFCO) 플레이트 및 유산균은 MRS( Lactobacilli MRS Broth; DIFCO) 플레이트와 m-LBS(modified-LBS ;DIFCO) 플레이트에 각각 0.1ml를 분주한 후, 도말하였다. PCA 플레이트는 35℃에서 24시간 배양하여 콜로니를 계수하여 측정하였고, PDA 플레이트는 25℃에서 48시간 배양 후 콜로니를 계수하였으며, MRS 플레이트(Lactobacilli species 배양배지)는 37℃에서 48시간 배양후 콜로니를 계수하였고, m-LBS 플레이트는 30℃에서 72시간 배양 후 콜로니를 계수하여 log CFU/g으로 나타내었다.10 g of each sample and 90 ml of sterile 0.1% peptone were placed in a stomacher bag and homogenized for 2 minutes using a stomacher (Lab blender, interscience, France) (DFTCO) plates, and Lactobacilli MRS Broth (DIFCO) plates and m-LBS (modified-LBS) plates were used as standard bacterial strains and PCA (plate count agar; DIFCO) plates, yeast fungus and potato dextrose agar DIFCO) plates were dispensed, and then plated. PCA plates were cultured at 35 ° C for 24 hours, and colonies were counted. PDA plates were cultured at 25 ° C for 48 hours, and colonies were counted. MRS plates (Lactobacilli species culture medium) The m-LBS plate was counted in log CFU / g after counting colonies after culturing at 30 ° C for 72 hours.
강화김치vitamin
Enhanced Kimchi
일반김치Control group
General kimchi
상기의 결과로부터 본 발명의 김치는 pH의 저하 속도와 산도의 증가 속도가 완만하게 나타나는 것을 알 수 있다. 또한 미생물의 증가 속도 중에서 특히 젖산균의 생육 증가 속도가 완만하게 진행되는 것을 알 수 있다. 그러나 일반김치는 pH의 저하 속도와 산도의 증가 속도가 빠르고, 미생물의 증식 속도도 빠르게 나타나는 것을 알 수 있다. 따라서 본 발명은 김치의 산패가 지연되고 상미기간이 증가되어 맛있는 김치를 제공할 수 있다. From the above results, it can be seen that the rate of decrease in pH and the rate of increase in acidity are gentle in the kimchi of the present invention. In addition, it can be seen that the growth rate of lactic acid bacteria grows slowly, especially in the increasing rate of microorganisms. However, in the case of ordinary kimchi, the rate of decrease of pH and the rate of increase of acidity are fast, and the growth rate of microorganisms is also rapidly appeared. Therefore, the present invention can provide a delicious kimchi by delaying the rancidity of Kimchi and increasing the freshness period.
<시험예 2>; 기능성 평가≪ Test Example 2 > Functional evaluation
1) 시료 전처리 1) Sample preparation
본 발명의 김치와 일반김치 각각 10 g을 80% 에탄올 100 ㎖을 첨가한 후 상온에서 12시간 교반 추출하였다. 여과한 후, 폴리페놀 함량을 측정하였으며, 항산화 활성은 여과액을 동결건조하여 사용하였다.10 g of each of the kimchi and ordinary kimchi of the present invention was added with 100 ml of 80% ethanol, followed by stirring at room temperature for 12 hours. After filtration, the content of polyphenols was measured. Antioxidant activity was determined by freeze drying the filtrate.
2) 총폴리페놀 함량 분석2) Total polyphenol content analysis
추출물 0.1 ㎖에 folin-ciocalteu reagent 50 ㎕를 첨가하여 혼합한 후 4분간 반응시킨 뒤, 20% sodium carbonate anhydrous 포화용액 1.5 ㎖을 첨가하였다. 2분간 반응 후 microplate reader (VERSAmax, Molecular Device, CA, USA)를 이용하여 760 nm에서 흡광도를 측정하였다. 총폴리페놀 함량은 chlorogenic acid를 이용하여 작성한 표준곡선으로부터 산출하였다.50 μl of folin-ciocalteu reagent was added to 0.1 ml of the extract, followed by reaction for 4 minutes and then 1.5 ml of 20% sodium carbonate anhydrous saturated solution was added. After 2 minutes, absorbance was measured at 760 nm using a microplate reader (VERSAmax, Molecular Device, CA, USA). Total polyphenol content was calculated from the standard curves prepared with chlorogenic acid.
본 발명의 김치가 대조구의 일반김치에 비하여 총 폴리페놀함량이 1.75배 높은 것으로 나타났다.The total polyphenol content of the inventive kimchi was 1.75 times higher than that of the control kimchi.
3) 항산화활성(DPPH radical 소거능)3) Antioxidant activity (DPPH radical scavenging ability)
메탄올에 0.4 mM의 농도로 용해한 DPPH 용액 160 ㎕와 시료 40 ㎕를 첨가하여 암소에서 30분간 방치한 다음 microplate reader (VERSAmax, Molecular Device, CA, USA)를 이용하여 515 nm에서 흡광도를 측정하였다. 양성 대조군으로 ascorbic acid를 사용하였다. 시료를 50,000 ㎍/㎖의 농도로 희석하여 항산화 활성을 측정한 결과 일반김치는 16%이고, 본 발명의 김치는 39%를 나타내었다. 따라서 일반김치에 비하여 본 발명의 기능성 김치는 2.4배 이상의 황산화활성을 나타낸다.After incubating for 30 minutes in a dark place, the absorbance was measured at 515 nm using a microplate reader (VERSAmax, Molecular Device, CA, USA). As a positive control, ascorbic acid was used. As a result of measuring antioxidative activity by diluting the sample to a concentration of 50,000 / / ml, the general kimchi was 16% and the kimchi of the present invention was 39%. Therefore, the functional kimchi of the present invention exhibits a sulfation activity of 2.4 times or more as compared with general kimchi.
상기의 결과로부터 본 발명의 김치는 6가지 원부재료인 고춧가루, 비타민C, 청국장, 청각, 녹차분말, 황태 육수 등 비타민을 강화시킨 김치조성물을 사용하여 담은 김치이므로 항산화활성이 우수한 것으로 나타났다.From the above results, the kimchi of the present invention showed excellent antioxidative activity because it was a kimchi prepared by using six kinds of raw materials, red pepper powder, vitamin C, chungkukjang, hearing aid, green tea powder,
4) 미백활성(Tyrosinase 저해활성)4) Whitening activity (tyrosinase inhibitory activity)
가. 분석방법 end. Analysis method
0.5M 인산완충용액(pH 6.5) 100 ㎕에 10 mM L-DOPA에 녹인 기질액에 40 ㎕ 및 시료용액 20 ㎕의 혼합액에 mushroom tyrosinase(110 U/㎖) 40 ㎕를 첨가하여 25℃에서 2분간 반응시켜 반응액 중에 생산된 dopachrome을 475 nm에서 측정하였다. 양성 대조군으로 kojic acid를 사용하였다.40 μl of mushroom tyrosinase (110 U / ml) was added to 100 μl of a 0.5 M phosphate buffer solution (pH 6.5) and 40 μl of the substrate solution and 10 μl of the sample solution were dissolved in 10 mM L-DOPA and incubated at 25 ° C. for 2 minutes And the resulting dopachrome was measured at 475 nm. Kojic acid was used as a positive control.
나. 분석결과I. Analysis
시료를 10,000~50,000 ㎍/㎖의 농도로 희석하여 측정한 결과, 시료의 색이 분석결과에 영향을 미치기 때문에 1,250~5,000 ㎍/㎖의 농도로 희석하여 측정하였다. 시료를 5,000 ㎍/㎖의 농도로 희석하여 측정한 결과 대조구의 일반김치는 23%의 미백활성을 나타내었으며, 본 발명의 기능성김치는 약 41%의 미백 활성을 나타내었다. 따라서 대조구의 일반김치에 비하여 기능성김치는 1.78배의 미백활성을 나타낸다. The samples were diluted to a concentration of 10,000 to 50,000 μg / ml, and the color of the sample was diluted to a concentration of 1,250 to 5,000 μg / ml in order to affect the analysis results. The samples were diluted to a concentration of 5,000 μg / ml. The results showed that the control kimchi had a whitening activity of 23%, and the functional kimchi of the present invention had a whitening activity of about 41%. Therefore, functional kimchi shows 1.78 times of whitening activity compared to normal kimchi of control.
이상과 같이 본 발명의 기능성김치는 항산화활성과 미백활성을 나타낼 뿐만 아니라, 아미노산의 함량이 높아 김치의 물성을 좋게 하면서, 김치의 산도를 낮게 유지시키며, 미생물이 장기간 동안 생육하므로 맛있는 김치를 제공한다. As described above, the functional kimchi of the present invention not only exhibits antioxidant activity and whitening activity, but also maintains the physical properties of kimchi with high amino acid content, maintains low acidity of kimchi, and provides a delicious kimchi since the microorganism grows for a long time .
<시험예 3>; 관능 검사≪ Test Example 3 > Sensory test
본 발명의 비타민C를 강화시킨 기능성 김치조성물로 만든 김치와 일반김치를 대상으로 조직감, 숙성도, 전체적인맛, 냄새, 기호도를 대상으로 5점척도법(5점; 아주우수, 4점; 우수, 3점; 보통, 2점이하; 미흡)으로 평가하여 아래의 표 6에 나타냈다. Panel은 식품에 조예가 깊은 대학생, 직장인 및 주부를 대상(20대, 30대, 40대, 50대 남녀 8명)으로 선발하였다.The 5-point scale method (5 points: excellent, 4 points: excellent, 3 points: excellent, 3 points: excellent, 3 points: excellent, Point, normal, 2 points or less; insufficient), and is shown in Table 6 below. The panel was selected for students (20s, 30s, 40s, and 8 boys and girls) who were proficient in food, college students, workers and housewives.
상기의 결과로부터 숙성기간이 길어질수록 전체적으로 품질은 떨어지는 것으로 나타났다. 그러나 일반김치에 비하여 그 속도가 지연됨으로써 맛있는 상미기간이 오랫동안 유지될 수 있다는 것을 알 수 있다. From the above results, the longer the ripening period, the lower the overall quality. However, it can be seen that the delicacy period can be maintained for a long time by delaying the speed compared with the ordinary kimchi.
종래에 배추의 팩틴과 섬유질 분해효소의 생육을 억제시키기 위하여 글리신 또는 알라닌 등의 아미노산을 첨가하는 대신에, 황태육수를 사용함으로써 팩틴과 섬유질분해효소의 활성을 억제시켜 김치의 조직감과 씹힘성 및 아미노산이 첨가되어 더욱 맛있는 김치를 제공할 수 있으므로 상미기간을 연장시킬 수 있다. 또한 항산화활성 및 미백효과의 기능성을 제공할 수 있다.Conventionally, instead of adding amino acids such as glycine or alanine in order to inhibit the growth of pectin and fibrotic enzymes of Chinese cabbage, the activity of pectin and fibrolytic enzymes is inhibited by using the radish juice to improve the texture and chewiness of kimchi, It is possible to add more delicious kimchi and thus to prolong the freshness period. It can also provide antioxidant activity and functionality of whitening effect.
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KR101946777B1 (en) | 2017-11-24 | 2019-02-12 | 한국식품연구원 | A method of reducing the color change of Kimchi and Kimchi with reduced color change |
KR102471921B1 (en) * | 2021-11-11 | 2022-11-29 | 윤임정 | Manufacturing method of kimchi using kombucha |
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KR100384309B1 (en) | 2000-12-27 | 2003-05-14 | 주식회사 효원메디푸드 | Functional Kimchi added green tea and process for preparation thereof |
KR100419666B1 (en) | 2003-07-11 | 2004-02-25 | 정화자 | Kimchi comprising fermented soybeans and making method thereof |
KR100820139B1 (en) | 2007-05-07 | 2008-04-08 | 신동식품(주) | A dressing materials containg walleye pollock gravy for kimchi and kimchi manufactured using the same |
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KR100384309B1 (en) | 2000-12-27 | 2003-05-14 | 주식회사 효원메디푸드 | Functional Kimchi added green tea and process for preparation thereof |
KR100419666B1 (en) | 2003-07-11 | 2004-02-25 | 정화자 | Kimchi comprising fermented soybeans and making method thereof |
KR100820139B1 (en) | 2007-05-07 | 2008-04-08 | 신동식품(주) | A dressing materials containg walleye pollock gravy for kimchi and kimchi manufactured using the same |
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Publication number | Priority date | Publication date | Assignee | Title |
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KR101946777B1 (en) | 2017-11-24 | 2019-02-12 | 한국식품연구원 | A method of reducing the color change of Kimchi and Kimchi with reduced color change |
KR102471921B1 (en) * | 2021-11-11 | 2022-11-29 | 윤임정 | Manufacturing method of kimchi using kombucha |
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