KR20170018532A - Method for producing cultured ginseng containing plenty of germanium and selenium - Google Patents
Method for producing cultured ginseng containing plenty of germanium and selenium Download PDFInfo
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- KR20170018532A KR20170018532A KR1020150112290A KR20150112290A KR20170018532A KR 20170018532 A KR20170018532 A KR 20170018532A KR 1020150112290 A KR1020150112290 A KR 1020150112290A KR 20150112290 A KR20150112290 A KR 20150112290A KR 20170018532 A KR20170018532 A KR 20170018532A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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Abstract
Description
The present invention relates to a method for producing ginseng root containing germanium and selenium, and more particularly to a method for producing ginseng root containing germanium and selenium by using sericite and sodium selenate.
Minerals are the five essential nutrients that are essential for life activities that control all the metabolism of the human body and are divided into inorganic minerals and organic minerals.
Recently, a lot of studies have shown that organic germanium and organic selenium play an important role in the body's immune system and their roles.
The germanium (Ge) is one of the carbon-group elements belonging to the 4B family in the periodic table of the elements. It was discovered by Winkler in Germany around 1886, and it was named Germanium after the name of its motherland. This germanium was first discovered in 1930 in the natural spring water of Lourdes, France, after the clinical report that it has therapeutic effect against various incurable diseases, and the germanium medical effect was found for the first time as a result of the composition analysis of spring water. In 1912, Nobel Prize laureate Alexis Karel After studying the spring water of the Lourdes region, the doctor also reported that the miracle of the Lourdes fountain was due to the presence of germanium
The germanium is classified into inorganic germanium and organic germanium. Inorganic germanium can not be consumed as toxic materials. It is widely used as a key material for future high-tech industries such as electronic industrial fields such as rectifier transistors and military missiles. It is also widely used in health care products. It was also reported that organic germanium is contained in plants such as ginseng, Ganoderma lucidum, garlic, aloe, comfrey, and myriamy. (In vitro culture and growth of germanium content of the genus Angelica). Wonkwang University Ph.D. )
This organic germanium is effective in lung cancer, bladder cancer, breast cancer, neurosis, asthma, diabetes, hypertension, etc. It is effective in the maintenance of nutritional balance in the body, weak alkalization of body fluids and oxygen supply, It was found to be effective. Therefore, it has recently been widely used for medical anticancer drugs, adult diseases prevention and treatment.
Organic germanium has been synthesized by Dr. Asai of Japan in the presence of a trace amount of germanium [GeCh₂CH2COOH] ₂O₃, which is called Ge-132, because it plays an important role in proper metabolism and health. , And then published a number of studies including biosynthetic organic germanium using yeast
On the other hand, selenium (Se) belongs to the 6B family in the Periodic Table of the Elements and was first discovered by Vercellius in Sweden in 1817. The World Health Organization (WHO) and the Food and Agriculture Organization of the United Nations (FAO) recognize that selenium is an essential nutrient, and the recommended daily dose is 50-200 μg per adult. However, the daily intake of Koreans is 43 μg I'm crazy. Therefore, people in Korea should take additional selenium. In particular, the recommended daily intake for cancer prevention and treatment purposes is over 500 μg. These selenium are contained in beer yeast, seaweed, seafood garlic, mushroom, tuna, bread, ham, algae, flour food, beef, chicken, milk and eggs but they are low in vegetables and fruits.
Selenium is a component of glutathione peroxidase, which is a powerful antioxidant enzyme that is distributed in the liver, kidney heart, and spleen of the body and eliminates harmful oxygen in the body. It lowers or eliminates the concentration of peroxides in cells , Immunity enhancing effect, anti-circulatory effect, and antiviral effect. In addition, it has the effect of prevention and treatment of cancer, liver regeneration of liver cirrhosis, delay of aging, etc., and functions to remove or decrypt heavy metals such as arsenic, mercury, lead and cadmium.
The selenium exists mainly in the form of seleno amino acid such as selenomethionine and selenocysteine, and organic selenium has a higher absorption rate in the body than inorganic selenium. The plant uses selenium absorbed from the soil to store the selenomethionine in the protein after biosynthesis. Since animals can not biosynthesize methionine, they take the vegetable and convert the selenomethionine from the liver to selenocysteine, which is used for protein synthesis or excreted out of the body. Selenium can reduce the body's intake of vitamin E and its antioxidant power is 2.000 times that of vitamin E. The deficiency of selenium mainly affects children and women of childbearing age, and can lead to heart disease, cancer immunity, erythropoiesis, myalgia, myocardial degeneration, and pancreatic function degeneration. In a report from the University of Arizona Cancer Center in the United States, research shows that selenium has a 37% reduction in cancer incidence and a 49% reduction in cancer mortality in the US elderly.
Accordingly, various prior arts for cultivating and producing plants having a high content of germanium (Ge) and selenium (Se) are disclosed.
First, Korean Patent No. 10-0413613 entitled " Method for mass production of germanium-containing plants by plant tissue culture method ", which comprises culturing the roots, stems and leaves of plants and growing them in germanium-containing medium, A method of producing a plant containing a large amount of germanium was proposed.
Korean Patent No. 10-0718720 entitled " Method for producing ginseng or ginseng adventitious roots containing organic germanium by cultivation with inorganic germanium addition liquid ", induces callus from tissue sections of ginseng or wild ginseng to form adventitious roots, A method of inoculating the proliferated irregular root into a predetermined medium to which inorganic germanium has been added and shaking the culture is proposed.
In Korean Patent No. 10-0481301 entitled " Method for growing crops containing plant-derived natural organic germanium ", water or an aqueous alkali solution is added to organic germanium or inorganic germanium at a ratio of 100: 1 to 10: , And the mixture is stirred at a temperature of 35 to 120 ° C. for 30 minutes to 24 hours to liquefy germanium in a state where the plants can be directly absorbed, and a step of adding sodium hypophosphite or Sodium hydroxide is added at a ratio of 1: 0.5 to 2 based on the amount of germanium, and the stabilized germanium solution is applied to the cultivar at a concentration of 2 to 50 ppm for about 1 to 20 times in the rhizosphere layer or the leaf surface of the soil .
Korean Patent Laid-Open Publication No. 10-2015-0052437 discloses a method for cultivating leaf vegetables using selenium-containing culture liquid, which comprises culturing at least one of sodium selenite and its hydrate, sodium selenate, 6.0 g, suggesting that the selenium component is organically incorporated into the plant tissue for 2 ~ 8 weeks at temperature 18 ~ 30 ℃, carbon dioxide concentration 600 ± 50ppm, humidity 60 ~ 75% Respectively.
Korean Patent Registration No. 10-0799652 entitled " Medium Composition for Production of Pleurotus oocyte Containing Selenium and Germanium and Method for Cultivating Nutri-Mushroom Using the Selenium and Ge-containing Composition of the Present Invention " refers to a mixed medium of selenium compound and organic germanium (Ge- To provide a technique to grow oyster mushrooms.
Korean Patent No. 10-0866587, entitled " Liquid fertilizer manufactured from the method of producing germanium and selenium-containing liquid fertilizer and cultivation method of crops using the same, " discloses a method of cultivating germanium and selenium-containing liquid fertilizer using germanium and SeO2 of clay species, And a method of cultivating the crops using the same.
However, some of the above-mentioned prior arts have a disadvantage in that the production cost increases due to the use of expensive organic germanium or that the prior art which does not use expensive organic germanium has a small effect of germanium or selenium.
Therefore, cultivation of germanium and selenium-rich crops is required without increasing the production cost.
Accordingly, it is an object of the present invention to provide a method for producing a ginseng root containing a large amount of germanium and selenium.
Another object of the present invention is to propose an eco-friendly cultivation method in which a ginseng cultivation germ containing a large amount of germanium and selenium is cultivated indoors without increasing the production cost, so that there is no residual pesticide component or heavy metal.
In order to accomplish the above object, the present invention provides a method for producing ginseng root containing germanium and selenium, comprising the steps of: preparing a 4/5 MS medium prepared with a nutrient medium; and pulverizing the sericite containing germanium, Or an aqueous sulfuric acid solution to ionize the germanium contained in the sericite and then adjusting the pH to 5.0 to 6.0 to prepare an ionized germanium solution; preparing a selenium component; Mixing 30-500 mg / l of the prepared ionized germanium solution and 30-500 mg / l of the prepared selenium component in the culture medium, and culturing the mixed culture medium with the ginseng cultured muscle tissue, .
The step of preparing the ionized germanium solution comprises the steps of: pulverizing the sericite containing germanium to 200 to 400 mesh; injecting a wood vinegar solution or an aqueous sulfuric acid solution into the powdered sericite; heating to 80 to 90 캜; stirring And aging ionizing for 3 to 5 days; and adjusting the pH to 5.0 to 6.0 by adding sodium hydroxide to the ionized solution.
The step of preparing the selenium component is characterized by preparing sodium selenate or sodium selenite pentahydrate.
The step of introducing the cultured ginseng cultured muscle tissue into the mixed culture medium comprises culturing the ginseng cultured muscle tissue by inducing kelosin from the wild ginseng sample and forming adventitious roots from the induced kelos, The cell tissue is added to the mixed medium by 5 to 20 g / L and cultured in an air incubator for 60 to 75 days.
According to the present invention, it is possible to supply ginseng cultured muscle having a high content of germanium and selenium at low cost, and it is applicable to various fields such as foods and cosmetics, thereby improving the immunity of users and preventing diseases.
In addition, there is an advantage that the reliability of the food can be enhanced by producing the safe wild ginseng culture root from pesticides and heavy metals.
Hereinafter, the present invention will be described in detail.
The present invention relates to a method for mass-producing ginseng culturing germs containing a large amount of germanium and selenium, and culturing the ginseng cultivation germ as the liquid culture medium.
That is, the present invention relates to a method for preparing a 4/5 MS medium prepared with a nutrient medium, a step of pulverizing the sericite containing germanium and then ionizing the germanium contained in the sericite by adding a vinegar solution or an aqueous sulfuric acid thereto, Preparing an ionized germanium solution by adjusting the pH of the prepared germanium solution to 5.0 to 6.0, preparing a selenium component, and adding 30 to 500 mg / l of the prepared ionized germanium solution to the prepared 4/5 MS medium, , And 30 to 500 mg / L of the components; and culturing the mixed culture medium by adding the culture medium of the ginseng cultured muscle cells.
Hereinafter, the present invention will be described in detail by steps.
Preparation of cell tissue of cultured ginseng root.
First, in the present invention, kelos is induced in the wild ginseng roots, i.e., wild ginseng samples, and adventitious roots are formed from the induced kelros to prepare ginseng cultured muscle tissue. Such a method for producing the cell tissue of the cultured muscle is in accordance with the Korean Registered Patent No. 10-0710857, which is a registered trademark of the present inventor.
In detail, first, the ginseng root, that is, the ginseng sample, is sterilized. As the disinfection method, the wild ginseng sample is placed in a beaker, and sterilized water and 2% sodium hypochlorite (NaOCl) are added thereto, stirred for about 10 minutes in a stirrer, and then washed three times or more with sterilized water. Was placed in a vacuum decompression cleaner in a clean bench, 70% ethanol was added, and the microbes and other contaminants existing between the roots were completely removed while generating air bubbles by vacuum decompression for about 15 minutes. Then, the ginseng samples were taken out and sterilized Use more than one cleaning method.
In addition, 3/4% of sucrose and 0.8% of agar were added to the culture media of MS ginseng samples, and the growth hormone of IBS 1ppm and / or 0.5g / l of coconut milk are further added, sterilized, and then 20 ml of Petri-dish is sterilized in a clean bench.
In addition, the ginseng samples were placed in a sterilized glass dish in a clean bench, and the husks of the sterilized methos roots were slightly peeled off. The pieces were cut into 2 to 3 mm size, and 4 to 5 teeth were placed in a petri dish with a culture medium. (parafilm), and incubated dark (dark) in an incubator at room temperature 23 ° C.
The primary identification and re-disinfection of the petri dishes of the wild ginseng samples is carried out at about 3 to 5 days after removal of the petri dishes cultivated in the incubator and the contamination status of bacteria and fungi by the naked eye is checked.
Next, a sample of a freshly cultured ginseng sample with no contamination is taken out, sterilized by immersing it in a 2% sodium hypochlorite solution for about 10 minutes, washed with sterilized water three times or more, incubated again in a new medium, and incubated in an incubator During the induction of the callus, the cultured petri dishes are taken out and the ultrasound is induced at 3 days intervals for 15 to 20 minutes at 68 KHz or higher for 3 to 4 weeks while inducing callus.
Then, the ginseng samples derived from callus (Miocyte differentiated cell mass) were taken out from the incubator to select good calli, and the selected calli were cut into less than 0.1 cm in sterilized meshes in a clean bench, and then inoculated into 4/5 MS medium . Or in liquid suspension medium, 2 ~ 3 weeks after inoculation, 4/5 MS medium and liquid suspension medium were inoculated. It is inoculated into a glass container for 2 liter of suspension medium.
After 2 weeks, the root of the wild ginseng root was removed from the Petri dish, and the superior culture medium was removed from the petri dish using a sterilized scalpel. The yellow part was cut to a length of 0.5 cm or less and further cultured in a 4/5 MS medium. And the cell tissue of the cultured ginseng root is used.
The compounding element names of the 4/5 MS medium used in the present invention are shown in Table 1 below.
Preparing 4/5 MS medium prepared with nutrient medium.
Next, 4/5 MS medium prepared with nutrient medium is prepared.
The mixing elements of the 4/5 MS medium are the same as those in Table 1 above. This 4/5 MS medium is a medium generally used for culturing the ginseng cultured muscle, and a detailed description thereof will be omitted. The production of the 4/5 MS medium is also in accordance with the present invention patent. The plant growth regulator IBA 1ppm and / or coconut milk 0.5g / l may be added, and if necessary, 0.05-0.5g / l of chitosan or chitooligosaccharide, and 0.05 to 0.1 g / l of chlorella.
Preparing a germanium ionized germanium solution by adjusting the pH to 5.0 to 6.0 after ionizing the germanium contained in the sericite by adding a vinegar solution or an aqueous sulfuric acid solution to the germanium-containing sericite powder;
Next, an ionized germanium solution is prepared.
The ionized germanium solution is prepared by: pulverizing sericite containing germanium at 200 to 400 mesh; injecting a wood vinegar solution or an aqueous sulfuric acid solution into the powdered sericite; heating to 80 to 90 ° C and stirring; Aging ionization for 5 days; and adjusting the pH to 5.0 to 6.0 by adding sodium hydroxide to the ionized solution.
That is, sericite (sericite) containing a germanium component is pulverized in a pulverizer to 200 to 400 mesh, most preferably 300 to 320 mesh, and the purified wood vinegar or 30% aqueous sulfuric acid solution is added to the powder. At this time, the amount of the purified wood vinegar solution or the aqueous 30% sulfuric acid solution is not limited, and may be about 1: 1 to 10 weight ratio of sericite, wood vinegar or aqueous sulfuric acid solution.
Then, the mixture is agitated at 80 to 90 ° C by heating in an agitator, and aged for 3 to 5 days to ionize germanium. Then, an ionized germanium solution is prepared by adding sodium hydroxide (NaOH) to the ionized solution to adjust the pH to 5.0 to 6.0.
The sericite used in the present invention is composed of about 60 natural minerals clay. The sericite used in the present invention does not contain heavy metals such as arsenic, lead, cadmium, and mercury and contains about 4 (mg / Kg) of germanium and releases anions and far-infrared rays. It shares the physical adsorption power and the polarity of chemical ions (+ -), which is washed with water, dried and then used.
Preparing the selenium component.
And prepare selenium components. Wherein the selenium component as may be used with commercially available sodium selenite (Na 2 SeO 4) or sodium selenite pentahydrate (Na 2 SeO 4 · 5 ( H 2 O)).
Mixing the prepared ionized germanium solution 30-500 mg / L and the prepared selenium component 30-500 mg / L in the prepared 4/5 MS medium.
Next, the prepared ionized germanium solution and the prepared selenium component, preferably sodium selenate or sodium selenate pentahydrate, are mixed in the prepared 4/5 MS medium. At this time, it is preferable to mix 30 to 500 mg / L of ionized germanium solution and 30 to 500 mg / L of selenium component. If the amount of germanium and selenium components contained in the produced ginseng culture muscle is low, And the amount of germanium and selenium contained in the wild ginseng culture muscle does not further increase even if the amount thereof is 500 mg / L or more, which is not economical.
And culturing the mixed ginseng cultured muscle tissue with the mixed medium.
Finally, 5-20 g / L of the ginseng cultured muscle tissue is added to the mixed medium and cultured in an air incubator for 60-75 days to finally produce ginseng cultured muscle.
At this time, the condition of the air incubator is not limited to the culture conditions of the known acid cultured muscle.
Hereinafter, specific examples of the present invention will be described.
(Example 1)
Preparation of the ginseng cultured muscle tissue.
Samples of wild ginseng were placed in a beaker, and sterilized water and 2% sodium hypochlorite (NaOCl) were added thereto. The mixture was stirred in a stirrer for about 10 minutes and then washed three times with sterilized water. Then, 4 / 5MS medium was prepared and 3% of sugar and 0.8% of agar were added. Then, 1 ppm of IBA and 0.5 g / l of coconut milk were further added, sterilized, and then 20 ml of Petri-dish was dispensed in a sterilized clean bench.
Next, sterilized wild ginseng samples were placed in a sterilized glass dish in a clean bench, and the husks of the sterilized methos roots were slightly peeled off. The pieces were cut into 2-mm pieces, and five teeth were placed in a Petri dish containing the culture medium to prepare parafilm ), And then incubated dark (dark) in an incubator at room temperature of 23 占 폚. For the first identification and re-disinfection of the ginseng samples, the petri dishes were taken out from the incubator for about 4 days, and the contamination status of germs and fungi was observed with the naked eye.
Then, samples of uncontaminated wild ginseng were taken out and dipped in 2% hypochlorous acid soda solution for about 10 minutes, washed with sterilized water three times or more, and then incubated again in a new medium and incubated in an incubator. During the callus induction process, the cultured petri dishes were taken out, and the callus was induced by ultrasonically vibrating for 3 to 4 weeks at 68 KHz or more for 15 to 20 minutes at intervals of 3 days.
Next, a sample of wild ginseng derived from callus (Miocyte differentiated cell mass) was taken out from the incubator to select good callus, and the callus was finely cut into 0.05 cm with a sterilized scalpel in a clean bench, and then inoculated on a 4/5 MS medium Respectively. After 2 weeks, the root of the wild ginseng root was removed from the Petri dish, and the superior culture medium was removed from the petri dish using a sterilized scalpel and cut into 0.5 cm length of the yellow part, followed by incubation for 2 weeks in 4/5 MS medium Cells of superior ginseng cultured muscle were used.
Preparation of mixed media.
4/5 MS medium is prepared with the same composition as that of the cell tissue. Then, the sericite containing germanium in Chungbuk area was thoroughly cleaned, dried, and pulverized into 300mesh. Then, 30% sulfuric acid aqueous solution was added at a weight ratio of 1: 2. The mixture was heated to 80 to 90 ° C and agitated for 3 days. Then, sodium hydroxide was added thereto to adjust the pH to 6.0. Here, the sericite used did not discharge heavy metals as shown in Table 2, and the content of germanium was 4 mg / kg.
Next, sodium selenite pentahydrate was prepared, and then the ionized germanium solution (200 mg / l) and sodium selenate sodium hydrate (200 mg / l) were mixed in 4 / 5MS medium.
culture
Finally, 20 g / L of the ginsenoside cultured muscle tissue was added to the mixed medium, and then cultured in an air incubator for 65 days.
(Comparative Example 1)
The same procedure as in Example 1 was carried out except that the ionized germanium solution and the sodium selenate pentahydrate were not used.
(Test Example 1)
Production and sensory evaluation of cultured ginseng root
The cultured muscles cultured through Example 1 and Comparative Example 1 were collected and the production amount and sensory evaluation of the cultured ginseng cultured muscle were carried out. The results are shown in Table 3 below.
/ Average weight (g)
As can be seen from the above Table 3, it was confirmed that the cultured ginseng cultured muscle of Example 1 was significantly higher than the cultured ginseng cultured muscle of Comparative Example 1. In the sensory test, it was also found that the wild ginseng root of Example 1 was darker in root color than the wild ginseng root of Comparative Example 1, had strong aroma, and had a bitter taste after taste. The sensory test was performed by five skilled sensual agents and the opinions of five sensual agents were combined.
(Test Example 2)
Measurement of crude saponin content of cultured ginseng
The crude saponin contents of Example 1 and Comparative Example 1 were measured. The results are shown in Table 4 below.
At this time, the crude saponin content was measured in accordance with the saponin test method for the food and drug safety notification, and the specific test method was as follows.
1 ~ 2 g of specimen is precisely weighed, placed in Erlenmeyer flask, dissolved in 60 ml of water, transferred to separatory funnel, washed with 60 ml of ether, and extracted three times with 60 ml of saturated butanol water. The extracts are combined and washed with 50 ml of water. Concentrate the water-saturated butanol layer in a con- centrated flask and concentrate it under reduced pressure. Dry it at 105 ° C for 20 minutes. Then cool it for 30 minutes in a desiccator and weigh the weight of the ginseng component (crude saponin).
Ginseng ingredient content (mg / g) = (A-B) / S
A: Weight (mg) of the flask after concentration and drying of the water saturated butanol layer
B: weight of an empty flask (mg)
S: Amount of sample (g)
However, in order to confirm the incorporation of components similar to ginseng components, the concentrated concentrate is dissolved in methanol and measured under the following conditions.
(Example of Measurement Conditions of High Performance Liquid Chromatography)
Column: for carbohydrate analysis
Solvent: acetonitrile, water, butanol (80:20:15)
Detector: RI
As can be seen from the above Table 4, it was confirmed that the crude saponin content of Example 1 was higher than the saponin content of Comparative Example 1.
(Test Example 3)
Measurement of Ge and Se contents in cultured ginseng cultivars
The Ge and Se contents of Example 1 and Comparative Example 1 were measured. The results are shown in Table 5 below. The Ge and Se contents were measured by the ICP-MS method of the Korea Food Research Institute.
As can be seen from the above Table 5, it can be confirmed that the Ge content and the Se content of Example 1 are significantly higher than those of Comparative Example 1.
As a result, the wild ginseng culture root according to the present invention is excellent in sensory evaluation such as color, taste and aroma, and also contains a great amount of crude saponin, germanium and selenium, and is cultivated indoors, It is safe for heavy metals and can be supplied at a low price, so that it can be utilized in various fields and has a merit of high added value.
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.
Claims (4)
Preparing an ionized germanium solution by adjusting the pH to 5.0 to 6.0 after ionizing the germanium contained in the sericite by adding a vinegar solution or an aqueous sulfuric acid solution to the germanium-containing sericite powder,
Preparing a selenium component,
Mixing the prepared ionized germanium solution (30-500 mg / L) and the prepared sodium selenate (30-500 mg / L) in the prepared 4/5 MS medium,
Culturing the mixed ginsenoside cultured cell tissue in a culture medium containing the ginseng and selenium; and culturing the cultured ginseng cultured muscle tissue.
Wherein preparing the ionized germanium solution comprises:
Pulverizing the sericite containing germanium to 200 to 400 mesh;
Adding a wood vinegar solution or an aqueous sulfuric acid solution to the powdered sericite, heating and agitating the mixture at 80 to 90 DEG C, aging and ionizing the mixture for 3 to 5 days,
And adding sodium hydroxide to the ionized solution to adjust the pH to 5.0 to 6.0. ≪ Desc / Clms Page number 24 > 6. A method for producing ginseng culture roots containing germanium and selenium.
Wherein preparing the selenium component comprises:
Characterized in that sodium selenate or sodium selenite pentahydrate is prepared.
The culturing of the mixed culture medium with the prepared ginseng cultured muscle tissue,
The ginseng cultured muscle tissue was prepared by inducing kelos in the wild ginseng samples and forming adventitious roots from the induced kelos. The prepared ginseng cultured muscle tissue was added to the mixed medium at a dose of 5-20 g / l, Lt; / RTI > culture medium containing germanium and selenium for 60 to 75 days in an air incubator.
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