KR20170003439A - Cosmetic composition containing Meranzin hydrate - Google Patents

Abstract

The present invention relates to a cosmetic composition. More specifically, the present invention relates to a cosmetic composition containing meranzin hydrate, exhibiting anti-inflammatory effects owing to meranzin hydrate contained in the cosmetic composition.

Description

The present invention relates to a cosmetic composition containing Meranzin hydrate,

The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition containing melanin hydrate and having an anti-inflammatory effect.

Skin is a covering covering the body, which physically and chemically protects the body from the outside world, and performs the biochemical function to metabolism of the whole body. If the skin is exposed to ultraviolet rays, tobacco smoke, or other pollutants, inflammation will occur and skin damage will result. Inflammation reaction reduces the water content of the stratum corneum and makes it dry, causing the elastic fibers of the dermal layer to denature, promoting skin aging. Accordingly, various cosmetic compositions have been developed as shown in the following patent documents in order to alleviate the inflammatory reaction and minimize skin damage.

<Patent Literature>

Patent Publication No. 10-1077824 (Announcement of Oct. 28, 2011) "Cosmetic composition for improving atopy containing sunlight extract and preparation method thereof"

However, conventional compositions using ilite, which is a mica-group mineral belonging to the monoclinic system, do not provide any guarantee of stability by using minerals or the like, and compositions using natural materials extracted from various plants (for example, chlorella Japanese Patent JP 10-36283 using extracts, etc.), there is a problem that the effect of alleviating the inflammatory reaction is deteriorated by placing the point of cell proliferation.

SUMMARY OF THE INVENTION The present invention has been made to solve the above problems,

It is an object of the present invention to provide a cosmetic composition having an anti-inflammatory effect.

In order to achieve the above object, the present invention is implemented by the following embodiments.

According to one embodiment of the present invention, the cosmetic composition according to the present invention is characterized by having an anti-inflammatory effect by containing merocyanine hydrate as an active ingredient.

According to another embodiment of the present invention, in the cosmetic composition according to the present invention, the above-mentioned melanin hydrate is contained in an amount of 0.001 to 30% by weight based on the total weight of the cosmetic composition.

According to the present invention, the following effects can be obtained by this embodiment.

In addition, the present invention inhibits the production of inflammatory factors and does not have cytotoxicity and thus has an anti-inflammatory effect.

1 is a graph showing the evaluation of the inhibitory effect of nitric oxide formation according to Example 2. Fig.
Fig. 2 is a graph showing the evaluation of TNF-a production inhibitory effect according to Example 3. Fig.
3 is a graph showing the evaluation of the inhibitory effect of IL-6 production according to Example 3. Fig.
4 is a graph showing the evaluation of inhibitory effect on IL-1? Production according to Example 3;

Hereinafter, a cosmetic composition containing melanin hydrate according to the present invention will be described in detail with reference to the accompanying drawings. Unless defined otherwise, all terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs and, if conflict with the meaning of the terms used herein, It follows the definition used in the specification. Further, the detailed description of known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted. Throughout the specification, when an element is referred to as "comprising ", it means that it can include other elements, rather than excluding other elements, unless specifically stated otherwise.

The cosmetic composition according to one embodiment of the present invention comprises melanin hydrate. The Meranzin hydrate has a molecular formula of C ₁₅ H ₁₈ O ₅ and has a structure represented by the following formula (1), and the CAS Registry Number is 5875-49-0, which is commercially available. However, the anti-inflammatory effect on the above-mentioned merocyanine hydrate was not known at all.

The cosmetic composition according to the present invention has an effect of inhibiting the production of inflammatory factors found in atopic and acne, including melanin hydrate.

In the present invention, the melanin hydrate is preferably contained in an amount of 0.001 to 30.0% by weight, preferably 0.01 to 10.0% by weight, based on the total weight of the cosmetic composition.

The cosmetic composition of the present invention may further comprise a composition selected from the group consisting of water-soluble vitamins, oiliness vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

As the water-soluble vitamin, any of vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C and vitamin H can be used, And salts thereof (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbic acid-2-phosphate etc.) can also be used as water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

As the above-mentioned useful vitamins, any vitamins A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) , And derivatives thereof (such as palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbic acid, dl-alpha tocopheryl acetate, dl-alpha tocopherol nicotinic acid vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, Ethyl ether, etc.) and the like are also included in the usable vitamins used in the present invention. The usable vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in a cosmetic, and preferably collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms .

The polymeric polysaccharide may be any compound as long as it can be compounded in cosmetics, and examples thereof include hydroxyethylcellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

The sphingogly lipid may be any as long as it can be compounded in cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. The sphingogly lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by a conventional method or can be obtained by chemical synthesis.

The seaweed extract may be any of those which can be compounded in cosmetics, but preferably include algae extract, red tiger extract, green algae extract and the like, and furthermore, coloring guanine, arginic acid, sodium alginate , Potassium alginate, and the like are also included in the seaweed extract used in the present invention. The seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic composition of the present invention may contain other ingredients usually added to the cosmetic composition. For example, it is possible to use various additives such as a preservative, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a plant extract, a pH adjuster, Cold agents, antiperspirants, purified water, and the like.

Examples of the above-mentioned fat-soluble ingredients include ester-based fats, hydrocarbon-based fats, silicon-based fats, fluorine-based fats, animal fats and plant fats and the like. Examples of the ester-based oil include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, But are not limited to, ethylbutyl, butylbutyl, ethylbutylbutyl, butylbutyl, isobutylbutyl, isobutylbutyl, isobutylisobutyl, isobutylisobutyl, isobutylisobutyl, Trimethylol propane, triisostearic acid trimethylol propane, tetra-2-ethylhexanoic acid pentaerythritol tri (2-ethylhexanoic acid) But are not limited to, tallow, caprylic estersyl, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, myristine sasyl, stearyl stearate, decyl oleate, Examples of the isocyanates include isophorone diisostearate, isophorone diisostearate, isophorone diisostearate, isophorone diisostearate, isophorone diisostearate, isophorone diisostearate, isophorone diisostearate, Ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol , Propylene glycol dicaprylylate, dicapric acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated acid glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyl Dodecylsuccinic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, Polyglycerin isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, octyldecyl lactate, octyldecyl isostearate, polyglycerin oleic acid ester, But are not limited to, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, Propyl stearate, propyl stearate, propyl stearate, stearyl stearate, stearyl stearate, propyl stearate, stearyl stearate, Stearyl hydroxystearate, stearyl 12-stearoyl hydroxystearate, 12-stearoyl hydroxystearic acid, Allo one hydroxy stearic acid and the like esters such as cetearyl source. Examples of the hydrocarbonaceous oil include hydrocarbons such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline. Examples of the silicone-based oil include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethyl polysiloxane, decamethyl polysiloxane, dodecamethyl cyclosiloxane, dimethyl siloxane methyl cetyloxysiloxane copolymer, dimethyl siloxane methyl stearoxysiloxane copolymer, Alkyl-modified silicone oil, and amino-modified silicone oil. Examples of the fluorine-based oil include perfluoropolyether and the like. Examples of the animal or plant oil include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, The present invention relates to a process for producing a starch-containing starch, which is selected from the group consisting of seed oil, cottonseed oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, Animal or vegetable fats such as oil, can-pick wax, carnauba wax, liquid lanolin, and hardened castor oil.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers. Examples of the water-soluble low molecular weight moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Propylene glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like. Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol, cholesterol ester and the like. Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid salt, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, . Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the molient agent include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants. Examples of the nonionic surfactant include self emulsifying monostearic acid glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene), sorbitan fatty acid ester, POE sorbit fatty acid ester POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POEPOP (polyoxyethylene polyoxypropylene) copolymer, POEPOP alkyl ether, polyether modified silicone, lauric acid alkanolamide , Alkylamine oxides, hydrogenated soybean phospholipids, and the like. Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylallylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, alkyl Amide phosphate, alkyloyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkylsulfosuccinate, sodium alkylsulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester and the like . Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium bromide, Benzalkonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like. Examples of the amphoteric surfactant include carboxybetaine, amidebetaine, sulfobetaine, hydroxysulfobetaine, amidosulfobetaine, phosphobetaine, aminocarboxylate, imidazoline derivative, amideamine, etc. , And the like.

Examples of the organic and inorganic pigments include silicic acid, anhydrous silicic acid, magnesium silicate, talc, sericite, mica, kaolin, spinach, clay, bentonite, titanium coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, Inorganic pigments such as aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium oxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

Examples of the organic powder include metal soaps such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of the ultraviolet absorber include ultraviolet absorbers such as p-aminobenzoic acid, ethyl p-aminobenzoate, amyl paranobenzoate, octyl p-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, Benzyl, 2-ethoxyethyl methoxycinnamate, octyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl methoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Dihydroxymethoxybenzophenone sulfonic acid, dihydroxybenzophenone sulfonic acid, dihydroxybenzophenone sulfonic acid and its salt, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzo Phenanone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino-p- (carbo-2'-ethylhexyl- 1,3,5-triazine, 2- (2- And the like can be de-hydroxy-5-methylphenyl) benzotriazole.

Examples of the bactericide include antibiotics such as hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, No. 301, mononitro and eicoll sodium, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, any of the above components may be blended within the range not impairing the object and effect of the present invention, but is preferably 0.01 to 5% by weight based on the total weight, More preferably 0.01 to 3% by weight.

The cosmetic composition of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients conventionally used in cosmetic compositions in addition to the above-mentioned compounds, for example, conventional additives such as stabilizers, solubilizers, vitamins, And a carrier.

The cosmetic composition of the present invention can be prepared in any formulations conventionally produced in the art, and examples thereof include emulsions, creams, foundations, lotions, essences, and hair cosmetic compositions. Specifically, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a moisturizing lotion, a nutritional lotion, a massage cream, a nutrition cream, a hand cream, a moisturizing cream, an essence, a pack, a soap, a lotion, a milk lotion, Cleansing foams, body cleansers, astringents, and spray formulations. When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component . When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether. In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters. When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used. When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these are only for the purpose of illustrating the present invention in more detail, but the scope of the present invention is not limited thereto.

(MTT assay), Nitric oxide production inhibition assay, pro-inflammatory cytokines (TNF-α and IL-6), and anti-inflammatory effects of melanin hydrate in the following examples. Evaluation of the inhibitory effect of production is examined, and a human skin patch test is conducted. RAW 264.7 cells, which are murine macrophage cell lines used in the following examples, were purchased from ATCC (American Type Culture Collection, VA, USA).

&Lt; Example 1 > Cytotoxicity evaluation (MTT assay)

(1) Cell Culture (Cell Culture)

RAW 264.7 cells were cultured in a DMEM medium supplemented with 10% FBS and 100 units / ml penicillin-streptomycin in a incubator maintained at 37 ° C and 5% CO ₂ and subcultured three times a day.

(2) To determine the cytotoxicity of Meranzin hydrate, MTT assay was performed. Cultured RAW 264.7 cells were co-cultured with LPS (1 μg / mL) and sample (Meranzin hydrate) for 24 hours. After the incubation, 10 μL of MTT solution was added and incubated for 2 hours. The supernatant was removed, and DMSO was added. After shaking well using a plate mixer, absorbance was measured at 540 nm. The cytotoxicity was evaluated in comparison with the control group treated with the stimulant. As a result, it was confirmed that no cytotoxicity was observed at concentrations below 200 μM of sample (Meranzin hydrate) in RAW 264.7 cells.

&Lt; Example 2 > Evaluation of anti-inflammatory effect ((Evaluation of Nitric Oxide Production Inhibitory Effectiveness)

(1) NO assay was performed to investigate the effect of nitric oxide production, which is one of the active oxygen species and plays an important role in inflammation induction. RAW 264.7 cells were adjusted to 1.5 × 10 ⁵ cells / mL using DMEM medium, then inoculated in 96-well plates and cultured for 24 h at the same time with fresh medium containing samples (Meranzin hydrate) and LPS (1 μg / mL) Respectively. The amount of NO produced was measured in the form of NO ₂ ^- present in cell culture medium using Griess reagent. 100 μL of the cell culture supernatant and 100 μL of the glycated reagent were mixed and reacted for 10 minutes, and the absorbance was measured at 540 nm using an ELISA reader. The amount of NO produced was compared with sodium nitrite (NaNO ₂ ) as a standard, and the results are shown in FIG.

(1) Referring to FIG. 1, it was confirmed that as the concentration of Meranzin hydrate increases from 50 to 200 μM, the NO production decreases. The above results show that Meranzin hydrate inhibits the production of NO, an inflammatory factor, and has an anti-inflammatory effect.

<Example 3> Evaluation of anti-inflammatory effect (Evaluation of the inhibitory effect of pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β)

(1) RAW 264.7 cells (1.5 × 10 ⁵ cells / mL) were inoculated into 96-well plates using DMEM medium and cultured in a 5% CO ₂ incubator for 24 hours. The medium was then removed and fresh medium containing sample (Meranzin hydrate) and stimulant LPS (1 ug / mL) was treated at the same time and cultured under the same conditions as pre-culture. After 24 hours, the culture medium was centrifuged (12,000 rpm, 3 minutes), and the content of pro-inflammatory cytokines produced in the supernatant was measured. The results are shown in FIGS. Quantification of pro-inflammatory cytokines was quantitated using a mouse enzyme-linked immunosorbent assay (ELISA) kit (R & D Systems Inc., Minneapolis, MN, USA).

2) As shown in FIGS. 2 and 3, it was confirmed that the amount of TNF-α, IL-6 and IL-1β was decreased with increasing concentration of sample at a concentration of 200 μM or less of the sample (Meranzin hydrate). The above results show that Meranzin hydrate inhibits the production of TNF-α, IL-6 and IL-1β, which are inflammatory factors, and has an anti-inflammatory effect.

<Example 4> Evaluation of anti-inflammatory effect (skin patch test)

(1) A cosmetic composition was prepared in the composition ratio shown in Table 1 below.

content(%) ingredient Cosmetic composition 1 Cosmetic composition 2 Sample (Meran's Hydrate) 0.05 - Vitamin E 0.2 0.2 Liquid paraffin 5 5 maintain 6.0 6.0 Ultraviolet absorber 2.0 2.0 Camellia oil 3.0 3.0 Flowing isoparaffin 2.0 2.0 antiseptic 0.01 0.01 Spices 0.5 0.5 Purified water Balance Balance Sum 100 100

(2) The antiinflammatory effect was confirmed in 20 persons who were suffering from inflammatory skin diseases such as atopy and acne. Twenty patients were divided into two groups and cosmetic compositions 1 and 2 were applied twice daily for 4 weeks to skin disease sites. The subjective evaluation of each participant in the skin condition was collected and shown in Table 2. As the evaluation items, significant improvement, general improvement, no effect, worsening, and marked deterioration were used.

Percentage of responses (%) Significant improvement General improvement no effect worse Significantly worse Cosmetic composition 1 30 60 10 0 0 Cosmetic composition 2 0 30 60 10 0

(3) From Table 2, it can be seen that the skin condition is significantly improved when the cosmetic composition 1 is used, compared with the cosmetic composition 2, which is determined depending on whether or not the cosmetic composition contains the melanin hydrate. That is, when the cosmetic composition contains melanin hydrate, the anti-inflammatory effect is excellent.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, Should be interpreted as belonging to the scope.

Claims (2)

A cosmetic composition for antiinflammation comprising melanin hydrate as an active ingredient. The method according to claim 1,
Wherein the melanin hydrate is contained in an amount of 0.001 to 30% by weight based on the total weight of the cosmetic composition.

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