KR20170002323A - Anti-aging cosmetic composition - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging. More specifically, the cosmetic composition for preventing skin aging contains collagen and wrinkle-amelioration peptide, and the collagen is made up of atelocollagen or succinylated atelocollagen collagen. The cosmetic composition for preventing skin aging shows excellent effectiveness in skin aging prevention, skin tissue regeneration, and skin wrinkle amelioration.

Description

ANTI-AGING COSMETIC COMPOSITION [0001]

The present invention relates to a cosmetic composition for preventing skin aging and improving skin wrinkles. More particularly, the present invention relates to a composition for preventing skin aging and improving skin wrinkles, which comprises atelocollagen or succinylated atelocollagen exhibiting an excellent effect on skin regeneration, To a cosmetic composition.

As the industry develops and the average life span of humans increases, there is a growing interest in the effects of aging on skin in relation to skin function and external beauty. Because the skin is directly in contact with the external environment, it is a complex organ composed of various cells and structures that play important barriers to protect the body from external factors including trauma, infection, and ultraviolet rays. The skin is largely composed of epidermis, dermis, subcutaneous tissue. The epidermis is composed of extracellular tissue proteins mainly composed of keratinocytes and pigmented melanocytes and Langerhans cells and the dermis is mainly produced by fibroblasts , And adipocytes (Sebocytes) are the framework of connective tissue. Type I collagen, the most abundant protein, has been shown to bind tissues with other types of extracellular tissue proteins besides Collagen, Elastin, Proteoglycan, Fibronectin, . The newly generated Type I procollagen is secreted into the extracellular space of the dermis and then undergoes fibrillogenesis to produce collagen that contributes elasticity and strength to the skin.

Skin aging is affected by genetic, environmental exposure (UV irradiation, mechanical shock), hormonal changes, metabolic processes (active oxygen species, reactive compounds such as sugars, aldehydes). These factors lead to accumulated changes in the structure, function, and appearance of the skin. Human skin aging is divided into internal aging that occurs over time according to two different biological processes, and external aging (photoaging) by environmental factors such as continuous light exposure, stress, and smoking. Clinically, it is characterized by thin, dry, pale, elastic, and fine wrinkles on internal aging skin, while skin with aging due to external environment such as light and smoking has deep wrinkles, spot coloration and rough skin And so on. Although chronologically aged skin and photoaged skin are morphologically distinct, their appearance is clearly distinguished, but recent research has shown that both aging promotes expression of collagenase (MMPs), decreased synthesis of procollagen, And signaling pathways such as damage to connective tissue. The environmental impacts are more obvious to the aesthetic quality or organization of the skin than the internal factors, the most lethal external factor being UV radiation. The coincidence of these molecular mechanisms suggests that UV irradiation is the chronological

Thereby accelerating important aspects of aging.

From a histological point of view, skin aging is caused by changes in the composition of extracellular matrix proteins that form the matrix in the dermis of the skin. Collagen proteins in the dermal extracellular tissues impart strength and tension to the skin, which protects the skin from external stimuli or forces. It accounts for 90% of the dermal layer. Collagen reduction is closely related to skin aging and wrinkle formation . The major cross-linking components of healthy dermis are type I collagen and type III collagen. However, in skin damaged by photoaging, the precursors of these two collagens are markedly reduced, and their reduction is associated with clinical severity. Collagen is degraded as it ages, but when it is exposed to ultraviolet rays, the biosynthesis of collagen degrading enzymes (MMPs) increases, collagen synthesis is reduced and wrinkles are formed.

Recently, a peptide derived from the C-terminal of human SNAP-25 protein, Faux-Tox, has been marketed as a skin wrinkle remedy, and peptides for improving wrinkles have been developed. However, since these peptides are too large in molecular weight and hydrophilic in many cases, they have many problems in transdermal absorption.

As a method for increasing the skin absorption of such a peptide wrinkle remedy, there is a method of grafting a long chain fatty acid such as palmitic acid at the terminal of the peptide in order to increase lipid solubility of the peptide (French Patent Application No. 2788058, WO 00/40611) was proposed, but its absorption enhancement effect was not significantly improved.

On the other hand, the inventors of the present invention have continued research on the development of cosmetics using atelocollagen by the Korean Patent Registration No. 10-828494.

International Patent Publication No. WO 00/40611 Korean Patent Registration No. 10-1068967 Korean Patent Registration No. 10-0828494

Accordingly, the present inventors have conducted intensive studies to further improve the skin wrinkle-improving effect by facilitating the skin absorption of peptides having the wrinkle-reducing effect, which has been conventionally developed. As a result, they have found that the use of atelocollagen It has been found that there is no skin irritation, the absorption power from the outer skin is enhanced, the skin is stable, and the effect of improving wrinkles in vivo is maximized. Thus, the present invention has been completed.

The object of the present invention is achieved by a cosmetic composition comprising atelocollagen or succinylated atelocollagen and a wrinkle-improving peptide.

The cosmetic composition of the present invention may further comprise hyaluronic acid. The cosmetic composition of the present invention may further comprise an amino acid.

The cosmetic composition of the present invention has excellent skin absorption rate and excellent wrinkle-reducing effect.

The atelocollagen used in the present invention is obtained by removing the telopeptide (Telo-petide) existing at both ends of the collagen by treating the type 1 natural collagen extracted from the animal with an enzyme (pepsin). The succinylated atelocollagen used in the present invention is obtained by reacting succinic anhydride with atelocollagen. In this case, the surface of the collagen can be said to be transformed into an electrically negative ion state.

By allowing anion to form in the collagen, the collagen dissolved in the acid solution can be dissolved in the neutral solution, which is a very important part of making cosmetics. Because cosmetics are applied to the human body, its acidity must be made close to neutrality. Therefore, collagen in which anion is formed has an advantage that it can be prepared in a neutral solution. It also has the advantage of increasing the penetration into the skin by forming ionic bonds with the skin. When hyaluronic acid is added to atelocollagen or succinylated atelocollagen, a synergistic effect with collagen can be obtained. Hyaluronic acid is a representative substance that forms a connective tissue together with collagen and chondroitin sulfate. For atelocollagen or succinylated atelocollagen, hyaluronic acid is added in a weight ratio of 0.1 to 2.0 per collagen.

The cosmetic composition of the present invention can be prepared by mixing atelocollagen or succinylated atelocollagen with a wrinkle-improving peptide. The wrinkle-improving peptide is added at a ratio of 0.01 to 0.5 in a weight ratio to collagen 1. [ The cosmetic composition of the present invention may contain a natural or synthetic amino acid which is effective for improving wrinkles. The kind of the amino acid is not limited, and a mixture of one or more kinds may be added. For example, lysine and adenosine may be added.

Adenosine, together with retinol, retinyl palmitate, and polyethoxylated retinamide, is the raw material for wrinkle improvement notices at the Korea Food and Drug Administration. This substance is a component of APT that is essential for the activity of a living organism. It is easily absorbed into cells and synthesized in vivo. It is an important substance that controls various metabolism. There is. Adenosine promotes DNA synthesis in dermal fibroblasts, increases protein synthesis, and increases cell size. Increases the synthesis of nucleic acids and activates protein metabolism to help maintain the skin's ability to survive in dying or depressed skin cells, improve cell function, and expand cell size to improve wrinkles due to destruction of dermal cells and aging It also gives vitality to the skin and enhances skin elasticity. In this case, the amount of amino acid added may be the same or different, and the total amount of amino acid is 0.01 to 0.5 for Atelocollagen 1. The cosmetic composition may be prepared in various formulations such as serum, essence, lotion, cream, lotion, etc., and may also be prepared in the form of a patch for a pack, for example.

In the case of preparing in the form of a patch, for example, after dissolving atelocollagen or succinylated atelocollagen in distilled water, separately preparing the porous layer and the hard layer, and then crosslinking the two collagen layers having different porosity, It can be manufactured in a lattice form which is not easily separated. The collagen membrane can be reinforced by mixing a dispersion of hyaluronic acid with a dispersion of atelocollagen or succinylated atelocollagen. By adding hyaluronic acid, it is possible to reinforce weak porous membranes and to improve cell migration upon binding with collagen fibers, thereby improving the efficacy. When the porous membrane thus prepared is used in place of the nonwoven fabric for pack to absorb the solution of the peptide and the amino acid and attach to the skin, the skin regeneration effect and the wrinkle reducing effect can be maximized.

Hereinafter, preferred embodiments of the cosmetic composition for wrinkle improvement according to the present invention will be described in detail as follows.

Example

1. Raw material mixing

0.39984 g of Atelocollagen, 79.968 g of 0.5% hyaluronic acid, and 19.59216 g of deionized water were placed in distilled water, and an impeller was placed and stirred for 24 hours. The pH was adjusted to 6.1 using 0.5 M NaOH and stirred for another 24 hours. Confirming that atelocollagen was dissolved, 0.0002 g of lysine, 0.04 g of adenosine and 0.04 g of wrinkle-improving peptide were added and stirred for 24 hours. The pH was adjusted to 6.1 using 0.5 M NaOH and the evaporated water was supplemented by addition of deionized water and stirred for an additional 15 minutes.

2. Wrinkle improvement performance evaluation

As a result of conducting the related test examples of the cosmetic composition for wrinkle improvement according to the present invention thus formed, it was found that there is no abnormality in the skin safety.

In the clinical evaluation, 20 women aged 30 or older who had normal eye wrinkles were selected and tested. The test was conducted twice a day (morning and evening) over 4 weeks, after cleansing, Were spread evenly over the entire area of the eyes and the whole face, and the wrinkles of the eyes were measured at 2 weeks and 4 weeks.

(1) wrinkle visual evaluation

At the evaluation stage of 2 weeks and 4 weeks after the product use, 20 testees were divided into 10 steps and the wrinkles of the right eye were evaluated and the average value was analyzed. 3.05% at 2 weeks after use, and 8.23% at 4 weeks after wrinkle reduction compared with before use.

(2) skin wrinkle measurement

Using PRIMOS compact at 2 weeks and 4 weeks after use, the skin wrinkles of the right eye were divided into four groups of Ra (arithmetic average roughness), Rmax (maximum height), Rz (maximum sectional height), and Rp (10 point average roughness) The average roughness and wrinkles of the skin were decreased. The roughness values Ra and Rz decreased by 4.59% and 8.23% after 2 weeks, respectively, and decreased by 16.21% and 22.85% after 4 weeks, respectively. The wrinkle was also significantly improved in Rp, which was 8.68% after 2 weeks and 19.16% after 4 weeks.

(3) Measurement of dermal density

Dermascopic densities of the right eye were measured using Dermascan C, an ultrasonic imaging device, at the evaluation points 2 weeks and 4 weeks after use. After 2 weeks of using the product, 5.11% improvement of the density of the dermal layer was observed at the evaluation point, and 9.89% at the evaluation point after 4 weeks.

(4) Analysis of skin deflection using facial contour image

As a result of analyzing the degree of angle change of the contour image, it was confirmed that it decreased by 7.23% after 2 weeks and by 9.33% after 4 weeks.

(5) Survey

Positive evaluation was increased in all items at 4 weeks after use. Especially, in 19 items, 95% of the total skin elasticity and elasticity improvement items were evaluated positively.

3. Skin safety evaluation

As a result of the safety evaluation, no stinging, erythema or keratin was observed in all 20 subjects.

The composition of the present invention prepared as described above is usually prepared in the form of serum, cream, lotion, essence and the like.

1. Serum

Raw material weight (% by weight)

Atelocollagen or succinylated atelocollagen 0.4

Hyaluronic acid 0.4

Cell regeneration peptide 0.04

Lysine 0.00002

Adenosine 0.04

Glycerin 7

Butylene glycol 5

Palmitoyl oligopeptide pentapeptide-32

Carbomer 0.2

Sodium hyaluronate 0.1

Panthenol 0.1

Allantoin 0.05

Squalane 5

Caprylic / Capric Triglyceride 4

PEG-60 Hydrogenated Castor Oil 2

Polysorbate 20 1.5

Sorbitan sesquioleate 0.8

Triethanolamine 0.2

Disodium EDTA 0.03

Antiseptic

Purified water balance

2. Cream

Raw material weight (% by weight)

Atelocollagen or succinylated atelocollagen 2

Hyaluronic acid 1

Cell regenerating peptide 1

Lysine 0.008

Adenosine 0.2

Natural amino acid 0.75

Carboxy polymer 0.3

1,3-butylene glycol 5

Glycerin 3

Jojoba oil 3

Stearic acid 2

Cetyl alcohol 2

Glyceryl monostearate 2

Polyoxyethylene sorbitan monostearate 0.5

Sorbitan sesquioleate 0.5

Glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate 1

Wax 1

Liquid paraffin 4

Squalane 2

Caprylic / Capric Triglyceride 3

Tocopheryl Acetate 0.2

Triethanolamine 0.5

Purified water balance

Claims (5)

Includes collagen and wrinkled paphed,
The cosmetic composition for preventing skin aging, wherein the collagen is composed of atelocollagen or succinylated atelocollagen.
The method according to claim 1,
The cosmetic composition for preventing skin aging further comprises hyaluronic acid.
The method according to claim 1 or 2,
The cosmetic composition for preventing skin aging further comprises an amino acid.
The method according to claim 1 or 2,
The cosmetic composition for preventing skin aging is prepared in one form selected from the group consisting of skins, creams, essences, serums, lotions and patches.
The method of claim 4,
Wherein the patch comprises an atelocollagen-based matrix.