KR20170000603U

KR20170000603U - Agarose gel for multi-channel pipette enabled gene electrophoresis

Info

Publication number: KR20170000603U
Application number: KR2020150005266U
Authority: KR
Inventors: 이강일
Original assignee: (주)바이오세상
Priority date: 2015-08-07
Filing date: 2015-08-07
Publication date: 2017-02-15

Abstract

The present invention proposes an agarose gel for gene electrophoresis capable of using a multi-channel pipette and reduces the number of electrophoresis of the gene on the agarose gel for electrophoresis by 12 times to 1, In the same way, the experiment time is reduced and the effect is simplified. Agarose gels for gene electrophoresis, which can use multi-channel pipettes that can reduce the number of user pipetting operations when loading a sample, provide user convenience (FIG. 2).

Description

Agarose gel for multi-channel pipette-enabled gene electrophoresis capable of using a multi-channel pipette [

The present invention relates to a loading baby rose for electrophoresis capable of using a multi-channel pipette, more specifically, to a well on an agarose gel for electrophoresis A multi-channel pipette can be used to prepare agarose gel wells, which can perform electrophoresis by loading 12 samples on a gel once, thereby improving the inconvenience of users who need to load 12 times. The present invention relates to an agarose gel for gene electrophoresis capable of using a pipette.

Gene electrophoresis is an experiment in which charged DNA is transferred to opposite polarity in an electric field using a chemical property of a gene having a negative charge (DNA) to separate the gene on the agarose. The rate at which the gene travels depends on several factors, including the size and shape of the gene, the pH and viscosity of the solution, the concentration of other electrolytes in the solution, the intensity of the ions, and the type of support. Thus, the rate of transfer of charged DNA in a solution in which DNA is dissolved is determined by the nature of the molecule itself. Therefore, the electrophoresis method is used as a very effective means for separating or analyzing charged substances such as genes, amino acids, nucleotides, etc. Such an electrophoresis apparatus largely consists of a standard electrophoresis apparatus and a gel electrophoresis apparatus.

In the standard electrophoresis apparatus, there is a gelcaster which is a standard type and can make and use an agarose gel well, and the size and the interval of the gel can not be controlled.

As described above, a gel electrophoresis apparatus required for electrophoresis of a gene such as DNA or RNA can be constructed by using an agarose gel, a buffer tank for electrophoresis, A pipette, a buffer for electrophoresis, and a gel loading buffer are required in addition to the above.

The agarose gel is prepared by adding an agarose powder to an electrophoresis buffer (TBE buffer or TAE buffer) according to the required amount and dissolving it by heating, then adding Ethidium Bromide (EtBr) And the mixture is poured into a gel cast and plugged with a well forming comb (not shown). The thus prepared agarose gel plate has a well capable of loading a sample , And the sample is loaded thereon. Alternatively, EtBr may be used by adding EtBr to a gel loading buffer or an electrophoresis tank buffer without adding it to the gel.

On the other hand, for the electrophoresis of the sample, a method of loading the sample on the agarose gel is as follows. A small amount of a gel loading buffer is loaded on a loading film, the sample is mixed with a pipette, and the sample is immersed in an electrophoresis buffer tank Of agarose gel

And then an electric field is applied. Such a gel electrophoresis apparatus is composed of a gel container and a cover. Two opposing electrodes are provided parallel to each other on both sides of the lower end of the gel container, and a gel bed is disposed at the bottom between the electrodes. A gel is placed on the gel bed. On the upper surface of the gel, a number of wells are formed in a direction perpendicular to the straight line connecting the electrodes.

The operation of the above-described gel electrophoresis apparatus will be described. A sample is placed in a gel well and the gel container is filled with a buffer (buffer). Then, a voltage is applied to both electrodes. The voltage is applied to the sample through the buffer solution, and the charged sample particle moves toward the electrode opposite to the charge through the buffer solution. At this time, the movement distance and the movement speed of the sample are different depending on the physical properties of the sample.

For example, a larger DNA molecule can not move farther from the gel well, and a smaller DNA molecule moves away from the gel well to form a DNA band of a larger size. Upon completion of the electrophoresis, the gel is irradiated with ultraviolet light to inspect the DNA band.

In the prior art relating to such an electrophoresis apparatus, U.S. Patent No. 6,379,519 discloses a disposable electricity specially designed for pre-cast polyacrylamide gels that are used simultaneously with electrophoresis of protein and sample (nucleic acid) Discloses a device for electrophoresis, but the design is not an agarose electrophoresis but a design for electrophoresis of a polyacrylamide gel.

U.S. Patent No. 4,391,688 relates to an electrophoresis apparatus using a horizontal slab gel and has a vessel filled at both ends with a buffer for electrophoresis. The buffer is filled in both vessels and a plate gel US Patent No. 4,207,166 discloses a device for electrophoresis comprising a membrane coated with an electrophoretic material such as agarose gel, plate. U.S. Patent No. 5,843,295 discloses a well-forming and loading-guiding comb that can be easily made into a well when making a gel for electrophoresis and can be easily loaded.

These patents relate to an apparatus and method for loading a sample into a well on an agarose gel in an electrophoresis buffer to electrophoresis. Such conventional agarose gels can not be processed using a multi-channel pipette because wells for loading a sample must be formed at predetermined intervals on an agarose gel using a well forming comb, and more than several thousand genes are electrophoretically analyzed In the field of genetic diagnosis, there is a difficulty for the operator to manipulate the pipet as many as the number of samples.

In order to solve the above-mentioned problem, the present invention has modified comb widths 30 and 30a of the wells for loading genes to prepare combs used for well formation (FIG. 2, comb).

Therefore, the object of the present invention is to prepare an agarose gel capable of using a multi-channel pipette, reduce the number of electrophoresis of the gene on the agarose gel for electrophoresis by 12 times to 1, And to provide an agarose gel which can simplify the production and reduce the number of pipetting operations of the user at the time of loading of the sample (Fig. 2).

In order to achieve the above object, the agarose gel for electrophoresis of the present invention is designed to have a comb shape with a width and an interval of a conventional comb so that a multi-channel pipette can be used (FIG. 1) (Fig. 2).

As shown in FIG. 1, the conventional combs for 12 well agarose gel preparation have a well and a well of 2.0 mm (FIGS. 1 and 20) and a well of 7.0 mm (FIG. However, the width of a conventional comb-forming agarose gel well is 7.0 mm, and it is impossible to use a multi-channel pipette (FIG. 3) if 12 wells are formed at a total agarose gel width of 10.7 cm.

However, the agarose gel for gene electrophoresis capable of using a multi-channel pipette according to the present invention has a well and an interval of 2.0 mm (Figs. 2 and 20a), and a width of 6.4 mm (Figs. 2 and 30a) To prepare agarose gel, 12-wells are formed at a total agarose gel width of 10.7 cm to use a multi-channel pipette (FIG. 3).

According to the present invention, an agarose gel capable of using a multi-channel pipette is manufactured, and the number of electrophoresis of the gene on the agarose gel for electrophoresis is reduced from 12 to 1, but the number of the electrophoresis genes is the same It has the effect of reducing the experiment time and simplifying it. It is possible to provide a user's convenience by providing an agarose gel which can reduce the number of pipetting operations of a user upon loading a sample (Fig. 2).

Fig. 1 shows the relationship between the agarose gel and the size
Fig. 2: Composition for preparing agarose gel according to the present invention and the size of the present invention agarose gel
3 A multi-channel pipette for use in the present invention agarose gel electrophoresis,
Fig. 4 shows the results of genetic electrophoresis using agarose gels and agarose gels prepared conventionally. When agarose gels prepared using conventional (Comb) combs were used, Electrophoresis However, when using the designed agarose gel, six gene electrophoresis is possible with one pipetting operation.

As shown in FIG. 1, the present invention is a well-known 12 well agarose gel preparation comb having a well and an interval of 2.0 mm (FIGS. 1 and 20) and a well width of 7.0 mm And the well interval is 2.0 mm (FIGS. 2 and 20a), and the width of the well is 6.4 mm (FIG. 2, 30a).

Agarose gel for gene electrophoresis capable of using multichannel pipettes was prepared by adding agarose powder to a buffer (TBE buffer or TAE buffer) according to the required amount and dissolving it through heating, and then adding ethidium (Ethidium Bromide, EtBr), a fluorescent sample for gene staining, etc., and then poured into a gel cast, followed by plugging a comb (not shown) according to the present invention, A gel plate for agarose gel for electrophoresis capable of using this designed multichannel pipette is provided with a well capable of loading 12 samples at a time, will be. Alternatively, a sample for gene staining may be used by adding a gene-staining sample such as EtBr to a gel loading buffer or an electrophoresis tank buffer without adding it to the gel. When the agarose gel for gene electrophoresis capable of using a multichannel pipette is manufactured, it is possible to use a multi-channel pipette (FIG. 3) by forming 12 wells in a total agarose gel width of 10.7 cm.

In conventional agarose gels, agarose gels having a predetermined width on agarose gels must be prepared using conventional well-forming combs (FIG. 1) for loading a sample (gene), so that operation using multi-channel pipettes is not possible However, in the present invention, in the case of genetic diagnosis in which thousands of genes are electrophoresed using agarose gel for gene electrophoresis capable of using a multi-channel pipette, a worker is required to experiment with only one-twelfth pipette operation And can be used effectively in the gene diagnosis industry by providing the convenience of saving the user's time and reducing the number of pipetting operations.

10 Agarose gel prepared by conventional comb
10a Agarose gel for gene electrophoresis capable of using a multi-channel pipette manufactured according to the present invention
20 Conventional agarose gel wells and wells
20a Well and well spacing of agarose gel for gene electrophoresis capable of using a multi-channel pipette manufactured according to the present invention
30 Well Width of Conventional Agarose Gel
30a The well width of agarose gel for gene electrophoresis capable of using a multi-channel pipette manufactured according to the present invention

Claims

(Fig. 2, 20a) and a width of 6.4 mm (Fig. 2, 30a) in a well and a well for a gel electrophoresis gel electrophoresis

The agarose gel for gene electrophoresis according to claim 1, which is capable of using a multi-channel pipette manufactured using Comb for preparing agarose gel for gene electrophoresis capable of using the multi-channel pipette