KR20160137105A - Functional food composition having an antidote of nicotine and its preparation - Google Patents

Abstract

A nicotine-detoxifying functional food composition comprises, as natural herbal ingredients, a Saposhnikovia divaricata extract, a Verbena officinalis extract, a Saururus chinensis Baill extract, a Maximowiczia Chinensis Rupreart var. typica Nakai extract, a green tea extract, a Glycyrrhiza uralensis Fischer et. De Candolle extract, a Pueraria thunbergiana extract, and a Platycodon grandiflorum extract; and further comprises, as fruit ingredients: a pear extract, a peach extract, a kiwi extract, and an aloe extract. The composition further comprises, as vegetable ingredients: an onion extract and a radish extract. Particularly, the nicotine-detoxifying functional food composition comprises: 10-100 mg of the Saposhnikovia divaricata extract; 35-200 mg of the Verbena officinalis extract; 5-500 mg of the Saururus chinensis Baill extract; 5-300 mg of the Maximowiczia Chinensis Rupreart var. typica Nakai extract; 5-1,000 mg of the green tea extract; 15-500 mg of the Glycyrrhiza uralensis Fischer et. De Candolle extract; 5-50 mg of the Pueraria thunbergiana extract; 5-50 mg of the Platycodon grandiflorum extract; 5-100 mg of the pear extract; 2-100 mg of the peach extract; 5-2,000 mg of the kiwi extract; 1-100 mg of the aloe extract; 3-100 mg of the onion extract; and 5-100 mg of the radish extract. The nicotine-detoxifying functional food composition shows excellent nicotine-detoxifying effects.

Description

TECHNICAL FIELD The present invention relates to a functional food composition for detoxifying nicotine,

TECHNICAL FIELD The present invention relates to a functional food composition for detoxicating nicotine and a method for producing the functional food composition, and more particularly, to a functional food composition having a detoxifying action of nicotine and tar containing a natural plant extract and a process for producing the functional food composition.

The present invention relates to a functional food composition having a detoxifying action such as nicotine and tar. The production of nicotine, tar, carbon monoxide and the like by smoking induces various diseases including lung cancer of the human body directly or indirectly, It has been recognized.

Currently, smoking is known to be one of the six leading causes of death worldwide, with 6 million deaths worldwide, and 2030 deaths totaling more than 8 million deaths per year (Mpower, 2008, WHO report on the global tobacco epidemic, WHO).

The smoking rate in Korea, especially the smoking rate of adult males, is high compared with the world. In 2005, the smoking rate of Korean males decreased from 52.3% to 43.7% in 2012. However, 132 countries (OECD health data, 2006), which is the 13th highest among countries in the OECD (Mpower, 2008, WHO report on the global tobacco epidemic, WHO).

Major diseases caused by smoking include lung cancer, chronic obstructive pulmonary disease (COPD), coronary artery disease, apoplectic ictus, caridioplegia, aortic aneurysm and circulatory system (Aortic and circulatory disease), phargngolaryngeal cancer, oral cavity cancer, etc. (Yoon, Seok-joon, 2001, Disease burden of premature death due to smoking in Korea, Preventive Medicine , 34, No. 3).

Tobacco smoke contains 4,000 toxic substances such as carcinogens harmful to human body. Among them, harmful main ingredient is tar and nicotine which is a mixture of various toxic substances.

Tar contains 2,000 kinds of toxic substances, and 20 kinds of toxic substances such as dioxin, benzopyrene, and dimethylnitrosamine are known to be carcinogens (Shin, Dong-cheon, Toxic Chemicals in Tobacco Smoke Substance, Korea Smoking Cessation Movement Council, 1999).

Nicotine is a lung cancer inducer, nitrosamine-4- (methylnitrosamino) -1- (3-pyridyl) -1-butanone {nitosamine-4- (methylnitrosamino) As a precursor of nicotine inhalation, it causes acute symptoms such as pupil dilation, visual acuity, vomiting, dyspnea, increased secretion from saliva and respiratory organs, reduced heart rate, and chronic symptoms such as arteriosclerosis, hypertension, various circulatory system Cancer Epidemiol Biomarkers Prev. 6: 113-20 (1997); Wynder (1997); < RTI ID = 0.0 & , EL et al., Environ. Health Perspect, 103 Suppl 8: 143-148 (1995)).

There are a myriad of patents related to nicotine-detoxifying or smoking-aid-assistant functional agents known so far, but representative examples are as follows.

1) Korean Patent Laid-Open No. 10-2001-0014750 (published on Mar. 26, 2001, Registered on Mar. 23, 2004), Registration No. 10-0446641 discloses a nicotine- Nicotine-decomposable drinks consisting of natural foods such as bank, celery, lemon and apple are introduced,

2) Korean Patent Application No. 10-2002-0069129 (published on Aug. 29, 2002, Dec. 21, 2004, Registration No. 10-0464140) discloses a functional preparation for nicotine degradation and a method for producing the same, A functional food composition which is expected to accelerate decomposition of nicotine, inhibit carcinogens and antioxidant effects containing a plant extract comprising green tea leaf, top leaf, bank, celery, lemon, apple, dermis and licorice,

3) Korean Patent Publication No. 10-2004-0077035 (published on Apr. 4, 2004, registered on Jan. 23, 2009, Registration No. 10-0547495) discloses a functional food composition having a cotinine inducing and antioxidative effect as a nicotine metabolite , A functional food consisting of extracts of green tea leaves, artemisia wilt, parsley, acanthopanax, quinceae, persimmon, persimmon and onion has been introduced,

4) Korean Patent Laid-Open No. 10-2004-0102690 (published on Dec. 2004, 2004) discloses a composition for detoxifying nicotine comprising a natural food extract as an active ingredient, wherein the composition is selected from the group consisting of Omiza extract, Extracts, and combinations thereof. The present invention relates to a plant and pharmaceutical composition for the detoxification of nicotine through oxidation, which comprises, as an active ingredient, a natural extract selected from the group consisting of extracts,

5) Korean Patent Publication No. 10-2005-0024884 (published on Mar. 11, 2005, published on Oct. 31, 2005, Registration No. 10-0526760) discloses an agent for reducing nicotine degradation effect and toxicity of dioxin An invention relating to a composition of a herbal medicine extract has been disclosed, which comprises a herbal medicine composition comprising black bean, porcine bean, gold euer, saury,

6) Korean Patent Laid-open No. 10-2006-0033345 (published on April 19, 2006, Registered on Sep. 18, 2007, Registration No. 10-0761552) discloses a herbal medicine extract for removing harmful components caused by smoking, In the present invention, a functional food containing aloe, marigold, chamomilla, japanese, tea tree, narcissus, ginseng, ginseng, licorice and herbal extracts has been introduced.

7) Korean Patent No. 10-0494223 (registered on Jun. 16, 2005) discloses a composite natural composition for detoxification of nicotine and tar, which is characterized in that it contains at least one selected from the group consisting of oriental herb, forage, green tea, white mug, licorice, (DTC ₉ ), which is composed of wood vinegar, wood vinegar and chitosan as active ingredients.

8) In recent years, Korean Patent Laid-Open No. 10-2011-0057103 (published on May 31, 2011, registered on Mar. 30, 2012 No. 10-1134200) discloses that smoking toxic detoxification The present invention relates to a pharmaceutical composition for use in medicine, which comprises an effective ingredient as an extract of at least one selected from the group consisting of a tomato prepuce extract, a whitewater extract extract, a twig extract, a dill extract and a duckwood extract, Powder or extract of clover, powder or extract of clover, powder or extract of luciferase, powder or extract of licorice, licorice powder or extract, powder or extract of clover, clove powder or extract, powder or extract of clover, aloe powder or extract, Stevia powder or extract or the like has been introduced,

9) Korean Patent Laid-Open No. 10-2012-0077900 (published on Jul. 10, 2012, registered on Oct. 29, 2013, Registration No. 10-1325122) discloses a composition for smoking cessation assistance having a nicotine- The present invention relates to a combination of a physiologically active composition consisting of coffee extract and coriander, turmeric, yuangbu, milky white, Saururus chinensis, Angelica gigas, Golden, Hwangjeong and Mint, as well as a polyphenolic system additive and a composition for smoking cessation supplement containing tobacco oil .

As described above, various functional foods containing a crude drug as a main ingredient have been introduced. However, the nicotine detoxifying effect obtained in actual use is often lower than the results shown in the experimental table, and steady research is urgently required.

It is an object of the present invention to provide a functional food having a nicotine-detoxifying effect as well as a nicotine-preventing effect.

In order to achieve the above object, the nicotine detoxifying food composition of the present invention is characterized in that it comprises at least one of windshield extract, In addition, the present invention further provides a composition containing a pear extract, a peach extract, a kiwi extract and an aloe extract as a raw material for fruits, and an onion extract and a radish extract as a raw material for vegetables.

The composition of the present invention is a material in which all the ingredient materials are stable within an allowable dosage range based on natural plants, and the ingredients are easy to purchase, and the composition of the present invention has excellent nicotine detoxifying action.

FIG. 1 is a graph showing the measurement of nicotine resolution to examine the amount of cotinine after mixing nicotine with a functional agent.
FIG. 2 is a graph showing the nicotine resolving ability of a cell treated with nicotine and then treated with the functional agent for nicotine degradation of the present invention. FIG.
3 is a graph showing the nicotine resolving ability of the functional preparation for nicotine degradation of the present invention using animal experiments.

The natural ingredients of the nicotine-detoxifying functional food composition of the present invention are as follows: a natural oriental herb contains a windshield extract, a Shinseongchu extract, a Saururus chinensis extract, an omija extract, a green tea extract, a licorice extract, a Puerariae radix extract, In addition, it is a composition containing a pear extract, a peach extract, a kiwi extract and an aloe extract as a raw material of fruit, and an onion extract and a radish extract as a raw material of vegetables. In particular, a composition of 10-100 mg of a windshield extract, 5 ~ 500 mg of extract, 5 ~ 300 mg of Omija extract, 5 ~ 1,000 mg of green tea extract, 15 ~ 500 mg of licorice extract, 5 ~ 50 mg of Puerariae Radix extract and 5 ~ This extract contains 2 ~ 100 mg of extract, 5 ~ 2000 mg of kiwifruit extract, 1 ~ 100 mg of aloe extract, 3 ~ 100 mg of onion extract and 5 ~ 100 mg of radish extract.

The method for producing a nicotine-detoxifying food composition according to the present invention comprises homogeneously mixing a raw material powder of a windbreak extract, a Shinseong herb extract, a Saururus chinense extract, an Omija extract, a green tea extract, a licorice extract, A method for preparing a nicotine detoxifying food composition, which comprises preparing a nicotine detoxifying food composition, which comprises preparing a mixture of 10 to 100 mg of a windshield extract, 10 to 100 mg of a cinnamon extract, a peach extract, a kiwi extract powder, an aloe extract, an onion extract and a radish extract powder, A raw material powder of 35 to 200 mg of extract, 5 to 500 mg of Saururus chinensis extract, 5 to 300 mg of Omija extract, 5 to 1,000 mg of green tea extract, 15 to 500 mg of licorice extract, 5 to 50 mg of Puerariae Radix extract and 5 to 50 mg of Ganoderma lucidum extract Were mixed homogenously and then added with 5-100 mg of a pear extract, 2-100 mg of a peach extract, 5-2,000 mg of an extract of kiwi, 1-100 mg of an aloe extract, 3-100 mg of an onion extract, And a method for producing a nicotine decryption functional food composition, characterized in that the prepared homogeneous mixture was added to the extract powder of 5 ~ 100 mg a feature of the technology configuration.

The composition of the present invention may contain a sweetening agent, a flavoring agent, a vitamin or a mineral, and the like in addition to the powder of the natural plant extract of the active ingredient, and may contain preservatives, emulsifiers or thickeners.

Hereinafter, active active herbs constituting the composition of the present invention will be described.

The windshield (Saphoshikovia divaricata, or siler divaricata) is a new year-old seedling of the daphniaceae (umbelliferae). It grows in the northeastern part of China, Faye Bay and Inner Mongolia. It is known that coumarin-based materials such as imperatorin, psoralen and bergapten are the main components of the windshield, and after the roots of the leaves that have not emerged in spring and autumn are dried and dried, I am using it. It is used for perspiration in one room, antipyretic, analgesic, shine, etc., and is also applied to inflammatory diseases.

Vervena officinalis has been used as a "holyherb" on the altar of the gods since ancient times and has the most magical horsepower in Europe, and it is known that it contains the glycosylated form of barbellin in the whole aeration, which causes inflammation, hemostasis and sedation. come. In addition, it relieves stress, depression, and tension, soothing and calming the seizure. It also has sweating, tonic, diuretic, and booster effects. It is good for menstrual irregularities and is also used as a remedy for liver diseases. Leaves are used as disinfectants for upper body dermatoses. Recently, it has been found that there is an effect of improving antioxidation and cell activity.

Saururus chinensis Baill. Or Houttunia cordata Thunberg is a perennial herbaceous plant belonging to Saururacea. It grows near the wetlands of Jeju Island. It is called Saururus chinensis because its leaves, flowers and roots are all white. When the flower of Saururus chinensis is taken out, it is dried out in the shade. Chinese herbal medicine has been introduced to treat the species and each, to make the feces come out well, to cut off the sputum, to pierce the clogs, to loosen the hard lumps in the stomach and to treat the boils and swellings. In addition, it has been introduced that Chinese medicine pharmacology dictionary, Chinese medicine dictionary, original pharmacology, Yeongnam medicine liquor rock, promotion of heading, etc., also fixes various species and each one, and is beneficial to feces, antibacterial, detoxification and anti-inflammatory action. It contains about 0.005% of essential oil during the outpouring, and the specific odor is attributed to decanoyl acetaldehyde and its oxidation products, ketones and aldehydes.

Ominja (Maxinowiczia Chinensis Rupreart var. Typica Nakai) is a deciduous, shrub-like shrub that grows on a mountain belonging to the Schizandracea. Its leaves are egg-shaped, phyllotaxic, leading edge, and pointed end. In summer, red and white flowers bloom, and the fruit is red with a small spherical shape. It is cultivated in various places except the central part of Korea. Dried roasted fruit of omija, it is sour and contains organic acid such as malic acid and tartaric acid, and it is widely used for convergence, calming, nourishment,

Green tea extract is a powder obtained by hydrothermal treatment of green tea leaves with purified water followed by freeze drying. The main components contained in green tea are catechin caffeine, free amino acids, vitamin C, vitamin E, beta-carotene, fluorine, zinc and the like minerals. Catechin has various physiological functions. The catechin contains flavonol-3-ol as a basic skeleton such as catechin, epicatechin, epigallocatechin, epicatechin gallate, The components such as epigallocatechin gallate, gallo catechin, gallocatechin gallate and catechin gallate have been identified. They have antioxidant activity, anticancer activity, angiotensin It has various physiologically active effects such as inhibition of conversion enzyme, promotion of alcohol metabolism, antimutagenesis, antihypertensive, antibacterial, reactive oxygen scavenging, cholesterol synergism, whitening action, lipid metabolism, The bar has been found.

Licorice (Glycyrrhiza uralensis Fischer et De Candole) is a perennial herb, belonging to the leguminosae, 60-100 cm high and 1-2 m long. Leaves are alternate phyllotaxis with odd-numbered icicles, and lobules are 4 to 8 pairs of elliptical, leading edge. In summertime, it has a pointed end, a butterfly-shaped light purple flower, a brown circumference, and the seeds are kidney-shaped. Lycopersicon esculentum or its roots and rootstocks, or peeled and dried, glycyrrhizin glycyrrhizin, glycyrramarin saponin, glycyrrhinarin, liquiritin, a flavonoid glycoside, sugar, resin, starch, Gums, essential oils, tannins, etc., and glycyrrhizin is hydrolyzed to glycyrrhetinic acid and two molecules of glucuronic acid. Liquorice is made with alleviating, germane, jinhae, and acne medicinal powders, fungicides, alcoholic agents, etc., and licorice powder is used as a loin, excipient and sweetener. In addition, it acts like a hormone against the secretory disorder of the corticosteroids of Pusan, and it is known to be effective for the treatment of gastric ulcer as well as detoxification of glucosin.

Pueraria thunbergiana is a dried root of a perennial perennial herb that belongs to beans and molluscs. Its length is 10 meters and all vines are covered with coarse hairs of tan. The roots of the root are thick, the leaves are long, It is a three-folded leaf. It is mainly used as a root, and isoflavone is mainly used. It contains daidzein, daidzin, puerarin, β-sitosterol, arachidic acid and a large amount of starch. It is known to have efficacy such as fever, fever, dyspepsia and headache, anemia, dysentery, stomach cramps, colds, colds,

Gakyung (platycodon grandiflorum Nakai) is commonly called bellflower and is widely used for edible and medicinal purposes. It belongs to the campanulaceae, and is distributed in Korea, Japan, China, etc. and it grows in the mountains. In spring and autumn, take roots, eat raw or eat as herbs. The main efficacy component of the bellflower is saponin. The general components of roots are mostly moisture, but they contain many carbohydrates and fibrin. Major components are aliphatic and hydrocarbons, and more than 100 components such as sulfides, furans, and acids have been identified. It is widely used for removing seawater or sputum caused by pulmonary disease in the oriental medicine. Especially, it has been reported that it is effective for central inhibitory action, soothing, analgesic and antipyretic action, and has blood pressure lowering and anticholinergic action.

The composition of the present invention contains, in addition to natural plants, pear, peach, kiwi and aloe fruit extract.

Pear (Pear) is divided into Western, Chinese, and Oriental Oriental Pear, each with different appearance and taste. In Korea, the southern type oriental pear is cultivated mainly, and imperial pear, blue pear, long pear, long pear and long pear are spread. Alcoholic food is mainly composed of carbohydrate, sugar (fructose and sucrose) 10 ~ 13%, organic acids such as malic acid, tartaric acid, citric acid, vitamin B and C, fiber and fat. It is effective for bronchial diseases from old days, and it is good for cold, relieve, asthma, and helps defecation and diuretic action. Removes sputum and coughs, alleviates symptoms when the throat is resting, or when the stomach is cold and sick, and helps to treat the boils. It has been reported that it also has a detoxifying action to eliminate hangovers.

Peach (Prunnus Persica, Peach) is also known as ceramics, known as eclipses and whites. It is used for diarrhea, diarrhea and constipation in oriental medicine. It is used for menstrual irregularity and dysmenorrhea. Main ingredients are water and sugar, and contains organic acids such as tartaric acid, malic acid and citric acid, and vitamin A and formic acid, It contains acetic acid, especially amino acid asparaginic acid. Alkaline food enhances immunity. It is good for development defect and night blindness. Skin is detoxifying. Organic acid removes nicotine to eliminate toxicity. It has also been reported that it contains a component that inhibits production.

Kiwi fruit is a filamentous fungus belonging to the genus Chrysanthemum. It originates from the coast of the Yangtze River in China and has been introduced to New Zealand in the 20th century. Its fruit shape is covered with brown hairs, juicy, sweet and sour taste is suitable and refreshing taste. Vitamin C, Vitamin E, Folic Acid, and Potassium, Calcium and Phosphorous Minerals. It can be recommended for children who have a lot of rickets and dyspepsia because of their immunostimulating effect. Also, Glutaric acid And arginine, and is rich in folic acid, which is said to be good for pregnant women.

Aloe (Aloe) belongs to the lily family (Liliaceae) and is a perennial plant. The spiky spines on both sides of the long, pointed leaves, usually like spears, and yellow or orange flowers bloom. Aloe gel powder obtained by concentrating and pulverizing aloe gel, aloe juice which is an extract of aloe leaves, and aloe powder which is dried and powdered after removing part that can not be used for food in aloe leaves are known as health functional foods. More than 80 kinds of active ingredients have been detected. Alloin and aloeimodine are good for the digestive system. Aloe tin acts as an antibacterial and antifungal agent. Aloe ulcine acts on ulcer. Aloe tin acts as an antitumor agent. Polymer polysaccharides are known to improve body fluids and have anti-cancer effects.

The vegetables used in the present invention may contain an onion extract and a radish extract.

Onion is known to originate in West Asia or the Mediterranean, and is mainly edible in the scaly stem. The distinctive odor from the scales stem is due to propyl disulfide, allyl sulfide, etc., and promotes digestive juice secretion, And so on. In addition, the scales stem contains various vitamins and minerals such as calcium and phosphorus, and it is known to have the action to remove harmful substances in the blood.

Radish (Raphanus sativa, Radish) is one of the three major vegetables with Chinese cabbage and red pepper. Its origin is Mediterranean and has a large columnar root. It was cultivated in Korea from the Three Kingdoms period. Ingredients include dietary fat, diazotas, and are known to have efficacy as a topical, a gadam drug, and a birth control pill.

Hereinafter, examples and experimental examples according to the present invention will be described.

Example  1: Functional formulation for nicotine degradation

1-1. Manufacturing stage of fruit and vegetable filtrate

30 kg each of freshly washed pear, peach, and aloe and 100 kg of kiwi were put into a grinder and pulverized and juiced. After squeezing, 100 L of water was added to the sludge, and the mixture was stirred for 1 hour. Then, the juice was squeezed and combined with the first juice. The juice was filtered through a 100 mesh screen and stored at 4 ° C.

1-2. Manufacturing stage of herbal medicine concentrate

500 L of water was added to the 1-ton tank, and the temperature was raised to 100 ° C. by adding 5 kg of windshield, 5 kg of ginseng, 25 kg of Saururus chinensis, 10 kg of saccharinic acid, 15 kg of Omija, 25 kg of licorice, 50 kg of green tea, And then extracted for 2 hours. The first extraction solution was separately stored and extracted, 500 L of water was added to the sludge, and the temperature was raised to 80 ° C and the second extraction was performed for 30 minutes. The primary extract and the secondary extract were combined and filtered through a 100 mesh screen and concentrated.

1-3. Mixing and spray drying step

The fruit and vegetable extract concentrate was uniformly mixed well, and the precipitate was removed using a high-speed centrifuge to prepare a nicotine decomposition product by a spray drying method.

Example  2: Functional beverage for nicotine degradation

A functional beverage was prepared by mixing 100 mg of water with 100 mg to 500 mg of the nicotine-degrading functional agent of Example 1 above.

Experimental Example  1: Direct mixing ( direct mixing ) To measure nicotine resolution

In order to analyze the presence of the nicotine degrading substance in the functional preparation for nicotine degradation prepared in Example 1, the nicotine decomposition function was measured by directly mixing nicotine with water and the functional formulation for nicotine decomposition. The water is a control. 200 μl of nicotine (nicotine (-), catalog number N3876) and 200 μl of functional agent solution (0.2%) were mixed well in Eppendorf tube (1.5 ml) The amount of cotinine produced after 20, 30, 60 and 120 minutes was measured by the corntin determination method and is shown in Fig . The measurement temperature was 25 占 폚. Quantitation of cotinine was performed in 1987 by Barlow et al. (Barlow, RD, Stone, RB, Wald, NJ, and Puhakainen, EJ (1987) Clin. Chim. Acta., 165, 45-52) Respectively. The concrete method is as follows.

In a 1.5 ml polypropylene tube, 200 μl of the sample and a reference sample were placed in a buffer solution or distilled water. Three tubes were used per sample to ensure the reliability of the experimental results. To the sample to be measured, 100 μl of 4 mM acetate buffer (pH 4.7), 40 μl of 1.5 M KCN, and 40 μl of 0.4 mM MCl 3-T dissolved in 50 mM of acetonitrile in 78 mM 200 μl of barbituric acid was added in order and mixed well for 10 seconds. The mixture was reacted at room temperature (25 ° C) for 15 minutes and then 40 μl of 1 M sodium metabisulphite was added to the reaction Lt; / RTI > Absorbance was measured at 490 nm and compared with reference cotinine.

Experimental Example  2: Measurement of nicotine resolution using cell culture

Cells were treated with a functional agent for nicotine and nicotine degradation to measure the cotinine produced by nicotine degradation. Nicotine has a half-life of about 30 minutes, but it has a half-life of more than 15 hours in the case of cotinine, so it can stably measure the transition of nicotine to the detoxified product. HepG2 cells derived from human hepatocytes were cultured for 1 day and further cultured for 1 day in nicotine-supplemented medium (Dulbecco's modified eagle medium, DMEM supplemented with 10% FBS, 1 mM nicotine) for 1 day. Cells were collected at 10, 20, 30, 60, and 120 minutes, respectively, while the functional agent (0.2%) was added to the cultured cells. The collected cells were washed 3 to 4 times with PBS (phosphate buffered saline) and scraped off using a scraper. Cells were harvested using centrifugation, 100 μl of PBS at 4 ° C was added, and the cells were disrupted with an ultrasonic pulverizer for 30 seconds. Then, the production of cotinine was measured at a wavelength of 490 nm using DBA (Direct Barbituric Acid) method. The same procedure was performed using water instead of the functional agent solution as a control.

FIG. 2 is a graph showing the nicotine resolving ability of a cell treated with nicotine and then treated with the functional agent for nicotine degradation of the present invention. FIG. As a result, it was found that the amount of cotinine produced in the case of applying the functional agent for the decomposition of nicotine of the present invention was higher than that in the case of applying water of the control group.

Experimental Example  3: Measurement of nicotine resolution using animal experiments

To investigate the effect of nicotine resolution on functional agents, experiments were conducted on SD rats (250 g, male, Samutoco, Korea). First, 20 SD rats were orally administered with 100 mg / kg of the functional composition of Example 2 and the same amount of the same amount of water for 3 days, and then oral administration of nicotine 0.5 mg / kg was repeatedly administered to the test rats. And the plasma was separated.

Each sample pretreatment method for analyzing the separated plasma was as follows. First, as a sample pretreatment method for quantifying cotinine, 25 μl of 10 M NaOH was added to 0.5 ml of plasma, followed by alkalinizing, followed by addition of 4 ml of dichloromethane (CH ₂ Cl ₂ ), followed by thorough mixing for 10 minutes to extract . The solvent was removed by a vacuum concentrator, and the residue was mixed with 100 μl of mobile phase to prepare a sample. As a sample pretreatment method for quantitation of nicotine, 50 μl of 10 M NaOH was added to 1 ml of plasma, and the mixture was alkalized. 4 ml of dichloromethane (CH ₂ Cl ₂ ) was added thereto, and the mixture was thoroughly mixed for 10 minutes. After centrifugation at 1000 g, 25 μL of concentrated hydrochloric acid was added, and the solvent was removed using a vacuum concentrator. The residue was mixed with 100 μL of the mobile phase to prepare a sample.

Cotinine analysis was performed using HITACHI L-2000 (HITACHI Co., Japan). As the assay conditions, 7% CH ₃ OH, 2 mM NaH ₂ PO ₄ , 0.2% phosphoric acid and 1 mM heptane sulfonate sodium were used as the mobile phase for quantitation of nicotine. to _{2% CH 3 OH, 2 mM} NaH 2 PO 4, 0.1% phosphate and 1 mM heptane sulfonic acid sodium salt was used. 80 쨉 l of the prepared sample was injected and detected at a flow rate of 1 ml / min at UV 260 nm. Nicotine was quantified by comparison with the peak height of acetanilide, and cotinine was calibrated with a standard curve with cotinine standard ((-) - Cotinine, Sigma, USA, C-0430). As a result, as shown in Fig. 3 , the functional agent was found to have reduced nicotine content and increased cotinine content as compared with the control group.

Claims (4)

Extracts of green tea, extract of licorice, extract of Puerariae radix, and extract of Gilchung as a raw material of natural oriental herbs, extracts of pear, peach, kiwi and aloe extract , And a composition containing the onion extract and the radish extract as a vegetable ingredient. The method according to claim 1, wherein the extract of 10 to 100 mg of the windshield extract, 35 to 200 mg of the extract of Shinseongcho, 5 to 500 mg of Saururus chinense extract, 5 to 300 mg of the extract of Omija, 5 to 1,000 mg of the green tea extract, 15 to 500 mg of the licorice extract, 5 to 50 mg of extracts and 5 to 50 mg of Ganoderma extracts, 5 to 100 mg of a pear extract, 2 to 100 mg of a peach extract, 5 to 2,000 mg of a kiwi extract, 1 to 100 mg of an aloe extract, 3 to 100 mg of an onion extract, Wherein the composition contains 5 to 100 mg of the extract. After mixing homogeneously the raw material powders of natural oriental herb extracts, Sinseongchu Extract, Saury Chrysanthemum Extract, Omiza Extract, Green Tea Extract, Licorice Extract, Puerariae Radix Extract and Gakyung Extract, the extracts of Pear, Peach Extract and Kiwi Extract Aloe extract, onion extract and radish extract powder are added and homogenized to prepare a nicotine-detoxifying functional food composition. 4. The method according to claim 3, wherein 10 to 100 mg of a windshield extract, 35 to 200 mg of a Shinsei primate extract, 5 to 500 mg of a Saururus chinense extract, 5 to 300 mg of an Omija extract, 5 to 1,000 mg of a green tea extract, 5 to 50 mg of the extract and 5 to 50 mg of the ginseng extract are mixed homogeneously and then 5 to 100 mg of the extract of the pear extract, 2 to 100 mg of the peach extract, 5 to 2,000 mg of the kiwifruit extract and 1 to 100 mg of the aloe extract 3 to 100 mg of an onion extract and 5 to 100 mg of a radish extract, and adding the resulting mixture to homogeneously mixed. The method for producing a nicotine-detoxifying functional food composition according to claim 1,

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