KR20160128847A - A system of gathering excreta from eel skin with extract mucin from excreta - Google Patents

A system of gathering excreta from eel skin with extract mucin from excreta Download PDF

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KR20160128847A
KR20160128847A KR1020150060890A KR20150060890A KR20160128847A KR 20160128847 A KR20160128847 A KR 20160128847A KR 1020150060890 A KR1020150060890 A KR 1020150060890A KR 20150060890 A KR20150060890 A KR 20150060890A KR 20160128847 A KR20160128847 A KR 20160128847A
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eel
skin
secretion
mucin
water tank
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KR1020150060890A
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KR101676318B1 (en
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김태석
정춘옥
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주식회사 리온메디코스
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/461Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish

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  • Proteomics, Peptides & Aminoacids (AREA)
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Abstract

The present invention increases the amount of skin secretion by applying an artificial stress to the skin of an eel, then scraping the secretion from the skin of the eel by a mechanical method, returning the eel to the aquarium by necessity, The present invention relates to a system for extracting mucin from a skin ejaculate collected from an eel by collecting a large amount of secretion from an eel skin to be used for recycling eel, An atmospheric water tank for storing the first time at the first water temperature by the first step and the second time at the second water temperature by the second step; The eel that is stuck in the atmospheric water tank is installed on the moving path, and a stress portion that applies a stimulus to the skin of the eel for a third time period by one or more of illumination, electric stimulation, and ultrasonic waves, And a circular tunnel part for collecting the secretion from the skin of the eel in contact with the skin of the eel while passing through the skin of the eel, thereby generating a large amount of secretion from the skin of the eel and extracting secretions from the skin, So that eel can be recycled and the production cost of mucin can be reduced.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a system for extracting mucin from eel dermis secretions obtained by collecting large amounts of secretions from eel skin,

The present invention relates to a system for generating a large amount of secretion from the skin of an eel and extracting mucin from the secretion collected from the skin of the eel. More particularly, the present invention relates to a system for extracting mucin from the skin of an eel by applying an artificial stress to the skin of the eel, The eel from the skin of the eel is scraped mechanically and the eel collected from the eel is returned to the water tank as needed to be recycled or used for edible purposes to produce a large amount of secretion from the eel skin that is recycled. Lt; RTI ID = 0.0 > mucus < / RTI >

Mucin is a type of glycoprotein contained in mucilages or secretions distributed in animals (animal) and animal and vegetable, and has a structure in which a sugar chain is linked to a peptide of a large core protein Neutral mucin and the like depending on the pH concentration, and it is classified into mucin (mucin) in human saliva, gastric mucosal secretion fluid, snail mucus (secretion) It is known that the content is high.

Vegetative mushins are derived from horses. The main source of vegetative mushin, Ma, is native to China and is taken from Dioscorea opposita Thunb, a perennial vine plant belonging to the genus Dioscoreaceae. Rounded hairs grow in the mountains and fields, and the roots of the roots are large and flat. The roots are long vines with branches. The leaves are alternate phyllotaxis, the petiole is long, triangular or heart shaped, and the length and the butterfly range from 4 to 13 cm . Leaf edges are plain, unbroken, without hair, and jujube (secondary stem of small scale) is formed on the axilla. The flowers of the horsetail are bloomed in June to July with unisexed flowers, and 1 to 3 horses are hanging on the axilla. The peduncle is short and the flower buds are 6 pieces.

The roots of tuna are edible, used as tonic and branch agents, and can be cultivated all over the country.

Animal mucin is mainly extracted from snails, but it has a problem of very small amount of harvest. It is known that mucin is contained in a large amount of eel skin-producing substance, so it is necessary to develop a technique of collecting mucin from eel skin secretion have.

Eel is an Anguilla japonica and its body length is about 50 to 60 centimeters. It is thin and elongated and is freshwater fish belonging to eel. The eel has a lot of body light, black and light. The eel is white and has no ventral fin. It has been known that the scales are buried in the skin. They live in fresh water for 5 to 12 years and then go down to the deep sea and lay eggs.

Eels are known to have high mucin content in the skin secretions of eel, and they are distributed in Korea, China, and Japan. They have good taste, nutritive value and are cultured.

Mucins are known to have effects such as moisturizing, antioxidation, wrinkle improvement, and atopic improvement of the skin. Therefore, cosmetics for skin beauty have been produced by utilizing extracted mucin.

Korean Patent Application No. 10-2013-0101779 (2013.08.27.) Discloses a method for producing a vegetable mucin fermented from horse mackerel, and a method for producing the same, A cosmetic composition excellent in wound regeneration ".

1 is a flow chart illustrating a method of collecting mucin according to an embodiment of the prior art.

The method for collecting mucins according to the prior art comprises the steps of (a) pulverizing hemp, (b) filtering crushed hemp, (c) precipitating a filtrate to obtain a supernatant, ) The resulting product is filtered, and (e) the filtrate is subjected to a fermentation step.

When fermenting in step (e), the filtrate is inoculated with lactic acid bacteria of the genus Lactobacillus and fermented, and the filtration in step (d) proceeds at 25 to 50 ° C for 20 to 100 hours.

The prior art has a problem in that it can not be applied to the extraction of mucins from the skin secretions of animal adult eel because it is a technique of collecting mucin from vegetable phage.

Therefore, there is a need to develop a technique for extracting mucins from the skin of animal adult eel.

Korean Patent Application No. 10-1993-0012590 (July 22, 1993) " Method for producing cosmetic using mucin component extracted from snail " Korean Patent Application No. 10-2013-0101779 (2013.08.27.) "A method for producing vegetable mucin fermented from horse mackerel and a cosmetic composition excellent in collagen synthesis performance, moisturizing, skin protection and wound regeneration,

In order to solve the problems and necessities of the prior art as described above, the present invention has been developed to apply stress to the skin of animal adult eel so as to increase the amount of secretion generated from the skin and to collect a large amount of skin secretions of eel It is an object of the present invention to provide a system for extracting mucins from eel dermis secretions produced in large quantities of eel skin excreta.

In addition, the present invention allows the eel to be kept alive after collecting the secretions from the skin of the eel, so that the eel can be recycled for the collection of the skin secretion, and the mucin is extracted from the eel skin secretion It is the purpose of the system to provide.

It is another object of the present invention to provide a system for extracting mucin from a mucus from a large amount of eel dermis secretion obtained by extracting purified mucin using the secretion collected from eel.

In order to accomplish the above object, the present invention provides a system for extracting mucin from eel dermis secretion produced from a large amount of eel dermis secretion, comprising a plurality of eels having a width of about 1.5 * 0.7 * 1 An atmospheric water tank in which the water is stored in a water tank of a meter size for a first time at a first water temperature by the first step and then for a second time at a second water temperature by a second step; A stressor installed in a path along which the eel held in the atmospheric water tank travels to apply a stimulus to the skin of the eel for a third time period by at least one of illumination, electric stimulation, and ultrasound; And a circular tunnel part through which one of the eels passed through the stress part comes in contact with the skin of the eel while scraping off secretions from the skin of the eel; . ≪ / RTI >

Wherein the stress portion is provided at an intermediate portion of the path, and the mucin sensor measures the amount of secretion or mucin from the skin of the eel; As shown in FIG.

A high-speed centrifugal separator for separating foreign matter from the circular tunnel portion using a centrifugal separator operated at 1,000 to 15,000 rpm; A mucin concentration unit for collecting the concentrated mucins by separating the mucin in a concentrated state by adding the secretory standard ethanol passed through the high-speed centrifugal separator in the range of 45 to 55 wt%; And a freeze-drying unit for obtaining mucin in a powder state by spraying the concentrated mucin from the mucin concentration unit at a temperature of minus 120 ° C to freeze-dry the mucin. As shown in FIG.

The atmospheric water tank is provided with one or more discharge holes and an inflow hole in the water tank to change or adjust the height of the water in the water tank, and at least one or more holes through which water can pass are formed in the water tank, The stress part is a path of a length of 2 meters in which an eel moves, and a 100-watt incandescent lamp at a distance of 1 meter from the upper part of the path is spaced at intervals of 50 centimeters along the longitudinal direction And an electric stimulation needle and an ultrasonic transducer are installed on the bottom surface, and the circular tunnel portion is a 1-meter-long cylindrical eel tunnel formed in a direction in which the eel moves, and is in direct contact with the circumference of the eel One or more rubber material scratches are repeated 3 to 5 times at uniform intervals along the longitudinal inner surface of the eel tunnel A plurality of holes through which collected secretions are formed may be formed at the bottom of the eel tunnel, and at least one of an electric stimulation needle and an ultrasonic vibrator may be installed.

Wherein the first water temperature is any value selected from the range of 20 to 25 degrees Celsius and the second water temperature is any value selected from the range of 5 to 10 degrees Celsius and the first time is selected from the range of 4 to 6 hours The second time is a value selected from a range of 10 to 20 minutes, and the third time is a value selected from a range of 3 to 10 minutes.

Wherein the first step is to maintain the water height of the water tank in the range of 70 to 80 centimeters when the eel is kept at the first water temperature in the water tank for the first time, The water level of the water tank can be maintained at 30 to 40 centimeters when the eel is kept for the second time.

The present invention having the above-described structure is advantageous in that a large amount of secretions are generated from the skin of the eel and a large amount of eel dermis is collected because stress is applied to the skin of the eel by using illumination, electricity, and ultrasonic waves.

In addition, the present invention having the above-described structure has the effect of reducing the production cost of mucin by reducing the production cost of eels for collecting skin secretions since the eels are collected after collecting secretions from the skin of the eel.

Meanwhile, the present invention having the above-described structure is advantageous in that it can efficiently extract and purify purified mucins using secretions collected from eel.

1 is a flowchart illustrating a method of collecting mucin according to an embodiment of the prior art,
FIG. 2 is a functional block diagram of a system for extracting mucin from eel dermis secretion collected by collecting a large amount of secretions from eel skin according to an embodiment of the present invention,
FIG. 3 is a detailed functional configuration diagram of an atmospheric water tank according to an embodiment of the present invention,
4 is a detailed functional configuration diagram of a stress part according to an embodiment of the present invention,
And
5 is a detailed functional configuration diagram of a circular tunnel according to an embodiment of the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and will be described in detail in the detailed description. It should be understood, however, that the invention is not intended to be limited to the particular embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

Mucin is a mucin substance secreted from the mucous membranes of living organisms, called mucin or point, difficult to dissolve in water and neutral salt solution, precipitated in acidic, alkaline and viscous liquid phase.

Mucins are largely classified into animal mucins and vegetable mucins, but the following description will be made with respect to animal mucins.

The chemical main body of mucin is a glycoprotein, which contains several glycoproteins depending on the origin of mucus. By way of example, there is a submandibular mucin, but the structure of the pig, It is very different. Other examples include stomach mucin, small intestine mucin, angiomyosin, cervical mucin, and the like. Digestive mucin protects organs and acts as a lubricant for digestive motility, and gastric mucin is used for gastric hyperplasia and gastric ulcer.

In addition, mucin contains chondroitin, a natural complex substance such as collagen, elastin, and protein, so it is said that mucin prevents moisture loss from external harsh environment and prevents drying of skin and quick regeneration of scarred skin.

Thus, mucin is a new substance that gives acne scars, sensitive skin regeneration and elasticity.

FIG. 2 is a functional block diagram of a system for extracting mucin from eel dermis secretion collected and collected by generating a large amount of secretion from eel skin according to an embodiment of the present invention. FIG. 4 is a detailed functional configuration diagram of a stressor according to an embodiment of the present invention. FIG. 5 is a detailed functional configuration diagram of a circular tunnel according to an embodiment of the present invention. FIG. to be.

The system 100 for extracting mucin from the eel dermis secretion collected from the eel skin by collecting and collecting a large amount of secretion from the eel skin is composed of an atmospheric water tank 1000, a stressor 2000, A circular tunnel section 3000, a high-speed centrifuge section 4000, a mucin concentration section 5000, and a freeze-drying section 6000.

The atmospheric water tank 1000 includes a plurality of eels of at least one eel stored in a water tank having a height of 1.5 * 0.7 * 1 meter in a first stage at a first water temperature for a first time, (One or more than one inlet hole 1200, one or more outlet holes 1300) and a flat plate shape, and one or more holes (through holes) are formed in the water tank 1100, And a lift steel plate 1400 which is raised and lowered by the corresponding operation.

The water tank 1100 has a length, height, and height of 1 meter * 0.5 meters * 1 meter, and one or more inflow holes 1200 for introducing water into the water tank 1100 And one or more discharge holes 1300 for discharging the water in the water tank 1100 to the outside are formed in the lower part or the lower part.

Experiments were conducted by injecting 50 adult eels into the water tank 1100. The steel plate 1400 is formed in a flat plate shape and has one or more through holes (holes) of a size free from the passage of water or through which the eel can not pass. The steel plate 1400 is connected to a device such as a string or gear, Or falls.

The lifting plate 1400 is operated to pull out or discharge the adult eel introduced into the water tub 1100 to the outside of the water tub 100.

The material of the water tank 1100 can be any one of stainless steel, wood, and plastics, but stainless steel is preferably used for uniform result and overall maintenance.

The water tank 1100 is filled with water for eel to a height of 70 to 80 centimeters for a first time by a first water temperature by a first step and water for an eel is filled by a second step for a second time by a second water temperature of 30 to 40 centimeters The water for the eel is filled.

Wherein the first water temperature is any one value selected from the range of 20 to 25 degrees Celsius and the second water temperature is any one value selected from the range of 5 to 10 degrees Celsius.

Also, the first time is any value selected from the range of 4 to 6 hours, and the second time is any value selected from the range of 10 to 20 minutes.

On the other hand, the first water temperature is preferably set to 21 degrees Celsius, and it is confirmed by experiments that the eel is stressed when it is set to be less than 20 degrees or more than 26 degrees. It is preferable to set the first time to 5 hours. If it is set to be less than 4 hours, the stabilization ratio in which the eel is adapted to the environment and is gentle is 70% or less, and if it exceeds 6 hours, .

The second water temperature is preferably set at 6 degrees Celsius. If the water temperature is set to less than 5 degrees or more than 11 degrees, it is confirmed that the eel is overcome the stress environment because there is no significant difference in the stress state in which the eel is subjected to stress. It is preferable to set the second time to 12 minutes, and it is confirmed that when the setting is set to less than 10 minutes and the setting time is more than 21 minutes, there is not much change in the stress state of the eel, thereby overcoming the stress environment.

In the first step of stabilizing the eel, 75 centimeters of the water in the water tank is preferable, and when it is less than 70 centimeters, it takes a lot of time to stabilize, and when it exceeds 81 centimeters, there is not a big difference.

Therefore, in the first step, the water temperature, which is the temperature of the water, is set at 21 degrees in the water tank 1100 having a width * length * height of 1 * 0.5 * 1 meter and staying for 5 hours stabilizes the eel in the optimum state . When changing the size of the water tank 1100 and the number of adults in the eel, it is very natural that all the experimental results should be re-examined.

In the second step of applying the primary stress to the eels, when the water height in the water tank 1100 is maintained at 30 to 40 centimeters, it is confirmed that the eels are adjacent to each other and are stressed with each other, The most stressful cases are identified. On the other hand, it is confirmed that the height of the water in the water tank 1100 is remarkably adapted when the height is kept below 30 centimeters or when the height exceeds 41 centimeters.

The eel, which is located inside the water tub 1100 and has undergone the first stress by the second step, moves to the stress part 2000 by the operation of the lifting plate 1400.

The stress unit 2000 is installed in a path along which the eel moves, and is configured to apply a stimulus to the skin of the eel for a third time period by any one or more of illumination, electric stimulation, and ultrasound.

On the other hand, the stress unit 2000 includes a mucin sensor not shown in the figure at an intermediate portion of the path to detect (measure) the amount of secretion or amount of mucin from the skin of the eel and to do. The control unit analyzes the detected data and determines whether mucin or secretion corresponding to the amount equal to or higher than the first reference level is secreted from the skin (epidermis) of the eel.

If the appropriate amount (dose) of secretion or mucin is not detected from the skin (epidermis) of the eel by the control unit in an appropriate capacity available for commercial use or above the first reference level, a stronger stimulation signal So that stronger stress is applied to the skin of the eel so that secretions or mucins are secreted from the skin of the eel.

In addition, the control unit analyzes the data detected by the mucin sensor and determines that secretions or mucins are secreted from the skin of the eel below the second reference level, which is difficult to commercially use from the skin of the eel, And when it is determined that the eels are moving, the travel path switching unit is driven so that the eels are returned to the waiting water tank. Although not shown in the drawing, the movement path switching unit can use or generally adopt a movement path switching structure of an eel.

The eel returning to the atmospheric reservoir returns to the skin through the first and second steps after releasing the secretion to the skin with time, and the amount of secretion or mucin is measured from the mucin sensor.

On the other hand, the above-mentioned 3 hours consisted of any one value selected from the range of 3 minutes to 10 minutes, and it was confirmed that maintaining the 5 minutes gave the greatest amount of stress to the eel and the amount of the secretion was great. It is confirmed that the amount of secretion decreases.

The stress unit 2000 has a length of 2 meters as a whole and 100 watts (W) of incandescent lamps 2100 are installed at intervals of 50 centimeters at a position of 1 meter on the upper side of the path. One or more The electric stimulation needle 2300 and the ultrasonic vibrator 2200 of the ultrasonic vibrator 2200 are installed along the longitudinal direction.

The incandescent lamp 2100 is configured to repeatedly flicker at a time value selected from the range of 0.2 seconds to 1 second. When the flashing is repeated at intervals of 0.4 seconds, the adult eel is the most stressed and moves actively .

The ultrasonic transducer 2200 can use one or more of the same or similar circular ultrasonic transducers for a general humidifier as the maximum output state and repeat the transverse or longitudinal direction.

The electric stimulation needle (2300) is a module structure that is protruded by a length of 2-3 millimeters in a unit area of 5 centimeters in width and 5 centimeters in length, and each needle is disposed at intervals of 1 centimeter in the front, back, left and right.

Applying a pulse signal having a frequency of 500 to 1000 hertz to each needle of the electric stimulating needles 2300 at a voltage of 5 to 6 volts (V) and a current of 1 to 1.5 amperes (A) causes the stress of the adult eel to be stressed It is confirmed that many secretions are discharged from the skin.

If the frequency of the signal applied to each needle of the electrical stimulation needle 2300 exceeds the range of 500 to 1000 hertz, exceeds the voltage range of 5 to 6 volts, or exceeds the current range of 1 to 1.5 amperes, The amount of the secretion collected is not greatly changed and it is confirmed that the efficiency is a problem.

The ultrasonic transducer 2200 and the electric stimulation needle 2300 may be alternately installed along the longitudinal direction of the stress portion 2000 or may be repeatedly installed by any one of them or alternatively, As shown in FIG.

In addition, it is preferable to control the stress intensity to gradually weaken within the allowable range from the inlet portion where the eel enters the stress portion 2000 to the exit portion where the eel comes out, in order to advance the eel.

The circular tunnel part (3000) is constituted to pass through the adult eel and contact the skin of the eel and scrape off the secretion from the skin of the eel.

The circular tunnel portion 3000 includes an eel tunnel 3100, a scraping portion 3200, a hole 3300, an electric stimulation needle 3400, an ultrasonic oscillator 3500, a support plate 3600, .

The circular tunnel part 3000 is installed on the eel exit side of the stress part 2000 and one or more of them are disposed adjacent to each other to improve the productivity of collecting the eel skin.

The circular tunnel part 3000 includes a 1-meter-long cylindrical eel tunnel 3100 formed in a direction in which an eel is moved and one or more hard rubber material scratches 3200 in direct contact with the eel's body circumference, 3100) at a uniform interval along the longitudinal inner surface.

Scratches (3200) are scaly and are arranged to maintain an inclination angle of 5 per 100 minutes in the direction of the eel, which contacts the skin of the eel and scrape the secretions from the skin of the eel in a mechanical manner, And is collected by the collecting tray 3700 through the hole 3300 while falling downward by gravity.

The inner bottom surface of the eel tunnel 3100 is relatively flat and a plurality of holes through which collected secretions are formed and at least one of the electric stimulation needle 3400 and the ultrasonic transducer 3500 is disposed in the width direction or the longitudinal direction Respectively.

When one or more of the electric stimulating needles 3400 and the ultrasonic transducer 3500 are installed in the longitudinal direction, the same position as the installation position of the scratching part 3200 is provided in three to five stages like the scratching part 3200 Is preferable for collecting secretions from the eel skin.

The support plate 3600 is in the form of a flat plate, and a hole 3300, an electric stimulation needle 3400, and an ultrasonic vibrator 3500 are formed or installed. The collecting tray 3700 is installed in a drawer shape capable of being drawn out at the lower end of the hole 3300 formed in the receiving plate 3600, and collects collected collected matter.

The eel passed through the circular tunnel part 3000 is kept in the living state since the secretion is removed from the skin (epidermis) and then the water is returned to the water tank. After about 2 hours, the eel skin is restored to the normal state Is confirmed. That is, there is an advantage that the eel can be recycled.

The high-speed centrifugal separator 4000 is operated by any one value selected from the range of 1,000 to 15,000 rpm by inputting the collected secreted material from the circular tunnel part 3000 and has a generally known constitution, Can be separated and removed.

The mucin concentration unit 5000 adds ethanol in a range of 45 to 55 wt% based on the weight of the secretion passed through the high-speed centrifugal separator 4000, reacts in a state of being stirred for 30 minutes to 1 hour and 20 minutes, Lt; RTI ID = 0.0 > mucin < / RTI >

That is, the substance precipitated or floated by the mucin concentration unit 5000 is collected in a state in which the mucin is concentrated and is generally known. The addition of ethanol at 50 wt% is the most effective way to minimize the time required for the separation of mucin into a concentrated state.

The freeze-drying unit 6000 is configured to obtain a mushin in powder form by spraying the concentrated mucin at a temperature of minus 120 degrees Celsius and freeze-drying it, and has a generally known chamber configuration.

Therefore, there is an advantage of efficiently extracting mucin from the secretions collected from the skin of the eel.

It is quite obvious that the composition of the present invention as described above can be applied to all mollusk animals other than eel.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art.

100: A system for extracting mucin from eel skin secretions collected from a large amount of secretion from eel skin
1000: atmospheric water tank 1100: water tank
1200: Inlet ball 1300: Outlet ball
2000: Stress part 2100: Incandescent light
2200: ultrasonic transducer 2300: electric stimulation needle
3000: Circular tunnel part 3100: Eel tunnel
3200: Scratch portion 3300: Hole
3400: electric stimulation needle 3500: ultrasonic vibrator
3600: Base plate 3700: Collection tray
4000: High-speed centrifuge unit 5000: Mucin concentration unit
6000: Freeze dryer section

Claims (11)

An atmospheric water tank in which a plurality of eels of at least one eel are allowed to stay in a water tank for a first time at a first water temperature and then for a second time at a second water temperature in a second step;
A stressor installed in a path along which the eel held in the atmospheric water tank travels to apply a stimulus to the skin of the eel for a third time period by at least one of illumination, electric stimulation, and ultrasound; And
A circular tunnel part for passing through one of the eels passing through the stress part and scraping off secretions from the skin of the eel by contacting the skin of the eel; Wherein the mucus is extracted from the eel dermis secretion collected from a large amount of secretion from the eel skin.
The method according to claim 1,
Wherein the stress portion is provided at an intermediate portion of the path, and the mucin sensor measures the amount of secretion or mucin from the skin of the eel; Wherein the mucus is extracted from the eel dermis secretion collected from the eel skin to produce a large amount of secretion from the eel skin.
3. The method according to claim 1 or 2,
A high speed centrifugal separator for centrifugally separating the secretion collected from the circular tunnel portion to separate foreign matter; Wherein the mucus is extracted from the eel dermis secretion collected from the eel skin to produce a large amount of secretion from the eel skin.
The method of claim 3,
A mucin concentration unit for collecting the concentrated mucin by separating the mucin into a concentrated state by adding ethanol to the secretion passed through the high speed centrifugal separation unit; Wherein the mucus is extracted from the eel dermis secretion collected from the eel skin to produce a large amount of secretion from the eel skin.
5. The method of claim 4,
A freeze-drying unit for obtaining a mucin in a powder state by spraying the mucin concentrated from the mucin-concentrated portion under a temperature condition of freezing and freeze-drying; Wherein the mucus is extracted from the eel dermis secretion collected from the eel skin to produce a large amount of secretion from the eel skin.
The method according to claim 4 or 5,
The atmospheric water tank is provided with one or more discharge holes and an inflow hole in the water tank to change or adjust the height of the water in the water tank, and at least one or more holes through which water can pass are formed in the water tank, Wherein the mucus is extracted from the eel dermis secretion collected from the eel skin to produce a large amount of secretion from the eel skin.
The method according to claim 6,
Characterized in that the stress portion comprises a structure in which an incandescent lamp or the like is provided along the longitudinal direction at an upper portion of a path through which an eel travels and at least one of an electric stimulation needle and an ultrasonic vibrator is installed on a bottom surface thereof A system for extracting mucin from a large amount of secretion produced and harvested from the skin.
8. The method of claim 7,
The circular tunnel portion is a cylindrical eel tunnel formed in a direction in which an eel is moved, and one or more scratches directly contacting the eel of the eel are formed in three to five repetitions at uniform intervals along the longitudinal inner surface of the eel tunnel Wherein at least one of at least one of an electric stimulation needle and an ultrasonic vibrator is provided at the bottom of the eel tunnel and at least one or more holes through which the collected secretion is transmitted is formed. And extracts mucin from the collected eel skin secretions.
9. The method of claim 8,
In the water tank, length values of length * length * height are 1.5 * 0.7 * 1 meter,
The centrifuge operates in the range of 1,000 to 15,000 rpm,
Wherein the freeze-drying unit is driven at a temperature of minus 120 degrees Celsius. A system for extracting mucin from eel dermis secretion collected and harvested by generating a large amount of secretion from eel skin.
10. The method of claim 9,
Wherein the first water temperature is a value selected from the range of 20 to 25 degrees Celsius,
The second water temperature is any one value selected from the range of 5 to 10 degrees Celsius,
Wherein the first time is a value selected from the range of 4 to 6 hours,
The second time is a value selected from the range of 10 to 20 minutes,
And the third time is a value selected from the range of 3 to 10 minutes. A system for extracting mucin from eel dermis secretion obtained by collecting and collecting a large amount of secretion from eel skin.
11. The method of claim 10,
The first step
Wherein the water level of the water tank is maintained at 70 to 80 centimeters when the eel is kept in the water tank for the first time from the first water temperature,
The second step
Wherein the water level of the water tank is maintained at 30 to 40 centimeters when the eel is kept in the water tank for the second time from the water tank to the second water temperature. Lt; / RTI >

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109601437A (en) * 2019-01-25 2019-04-12 淮海工学院 The method that Mucilage of loach stress is secreted and collected
WO2021167311A3 (en) * 2020-02-17 2021-10-14 이성규 Enzyme food composition for ameliorating obesity and skin wrinkles, comprising fast-fermented rhynchosia nulubilis paste and eel mucus

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KR20130101779A (en) 2012-03-06 2013-09-16 (주)유성글로벌 Hybrid virtual disk service system

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Publication number Priority date Publication date Assignee Title
KR20130101779A (en) 2012-03-06 2013-09-16 (주)유성글로벌 Hybrid virtual disk service system

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109601437A (en) * 2019-01-25 2019-04-12 淮海工学院 The method that Mucilage of loach stress is secreted and collected
CN109601437B (en) * 2019-01-25 2021-06-15 淮海工学院 Method for stress secretion and collection of loach mucus
WO2021167311A3 (en) * 2020-02-17 2021-10-14 이성규 Enzyme food composition for ameliorating obesity and skin wrinkles, comprising fast-fermented rhynchosia nulubilis paste and eel mucus

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