KR20160128847A - A system of gathering excreta from eel skin with extract mucin from excreta - Google Patents
A system of gathering excreta from eel skin with extract mucin from excreta Download PDFInfo
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- KR20160128847A KR20160128847A KR1020150060890A KR20150060890A KR20160128847A KR 20160128847 A KR20160128847 A KR 20160128847A KR 1020150060890 A KR1020150060890 A KR 1020150060890A KR 20150060890 A KR20150060890 A KR 20150060890A KR 20160128847 A KR20160128847 A KR 20160128847A
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- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
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Abstract
The present invention increases the amount of skin secretion by applying an artificial stress to the skin of an eel, then scraping the secretion from the skin of the eel by a mechanical method, returning the eel to the aquarium by necessity, The present invention relates to a system for extracting mucin from a skin ejaculate collected from an eel by collecting a large amount of secretion from an eel skin to be used for recycling eel, An atmospheric water tank for storing the first time at the first water temperature by the first step and the second time at the second water temperature by the second step; The eel that is stuck in the atmospheric water tank is installed on the moving path, and a stress portion that applies a stimulus to the skin of the eel for a third time period by one or more of illumination, electric stimulation, and ultrasonic waves, And a circular tunnel part for collecting the secretion from the skin of the eel in contact with the skin of the eel while passing through the skin of the eel, thereby generating a large amount of secretion from the skin of the eel and extracting secretions from the skin, So that eel can be recycled and the production cost of mucin can be reduced.
Description
The present invention relates to a system for generating a large amount of secretion from the skin of an eel and extracting mucin from the secretion collected from the skin of the eel. More particularly, the present invention relates to a system for extracting mucin from the skin of an eel by applying an artificial stress to the skin of the eel, The eel from the skin of the eel is scraped mechanically and the eel collected from the eel is returned to the water tank as needed to be recycled or used for edible purposes to produce a large amount of secretion from the eel skin that is recycled. Lt; RTI ID = 0.0 > mucus < / RTI >
Mucin is a type of glycoprotein contained in mucilages or secretions distributed in animals (animal) and animal and vegetable, and has a structure in which a sugar chain is linked to a peptide of a large core protein Neutral mucin and the like depending on the pH concentration, and it is classified into mucin (mucin) in human saliva, gastric mucosal secretion fluid, snail mucus (secretion) It is known that the content is high.
Vegetative mushins are derived from horses. The main source of vegetative mushin, Ma, is native to China and is taken from Dioscorea opposita Thunb, a perennial vine plant belonging to the genus Dioscoreaceae. Rounded hairs grow in the mountains and fields, and the roots of the roots are large and flat. The roots are long vines with branches. The leaves are alternate phyllotaxis, the petiole is long, triangular or heart shaped, and the length and the butterfly range from 4 to 13 cm . Leaf edges are plain, unbroken, without hair, and jujube (secondary stem of small scale) is formed on the axilla. The flowers of the horsetail are bloomed in June to July with unisexed flowers, and 1 to 3 horses are hanging on the axilla. The peduncle is short and the flower buds are 6 pieces.
The roots of tuna are edible, used as tonic and branch agents, and can be cultivated all over the country.
Animal mucin is mainly extracted from snails, but it has a problem of very small amount of harvest. It is known that mucin is contained in a large amount of eel skin-producing substance, so it is necessary to develop a technique of collecting mucin from eel skin secretion have.
Eel is an Anguilla japonica and its body length is about 50 to 60 centimeters. It is thin and elongated and is freshwater fish belonging to eel. The eel has a lot of body light, black and light. The eel is white and has no ventral fin. It has been known that the scales are buried in the skin. They live in fresh water for 5 to 12 years and then go down to the deep sea and lay eggs.
Eels are known to have high mucin content in the skin secretions of eel, and they are distributed in Korea, China, and Japan. They have good taste, nutritive value and are cultured.
Mucins are known to have effects such as moisturizing, antioxidation, wrinkle improvement, and atopic improvement of the skin. Therefore, cosmetics for skin beauty have been produced by utilizing extracted mucin.
Korean Patent Application No. 10-2013-0101779 (2013.08.27.) Discloses a method for producing a vegetable mucin fermented from horse mackerel, and a method for producing the same, A cosmetic composition excellent in wound regeneration ".
1 is a flow chart illustrating a method of collecting mucin according to an embodiment of the prior art.
The method for collecting mucins according to the prior art comprises the steps of (a) pulverizing hemp, (b) filtering crushed hemp, (c) precipitating a filtrate to obtain a supernatant, ) The resulting product is filtered, and (e) the filtrate is subjected to a fermentation step.
When fermenting in step (e), the filtrate is inoculated with lactic acid bacteria of the genus Lactobacillus and fermented, and the filtration in step (d) proceeds at 25 to 50 ° C for 20 to 100 hours.
The prior art has a problem in that it can not be applied to the extraction of mucins from the skin secretions of animal adult eel because it is a technique of collecting mucin from vegetable phage.
Therefore, there is a need to develop a technique for extracting mucins from the skin of animal adult eel.
In order to solve the problems and necessities of the prior art as described above, the present invention has been developed to apply stress to the skin of animal adult eel so as to increase the amount of secretion generated from the skin and to collect a large amount of skin secretions of eel It is an object of the present invention to provide a system for extracting mucins from eel dermis secretions produced in large quantities of eel skin excreta.
In addition, the present invention allows the eel to be kept alive after collecting the secretions from the skin of the eel, so that the eel can be recycled for the collection of the skin secretion, and the mucin is extracted from the eel skin secretion It is the purpose of the system to provide.
It is another object of the present invention to provide a system for extracting mucin from a mucus from a large amount of eel dermis secretion obtained by extracting purified mucin using the secretion collected from eel.
In order to accomplish the above object, the present invention provides a system for extracting mucin from eel dermis secretion produced from a large amount of eel dermis secretion, comprising a plurality of eels having a width of about 1.5 * 0.7 * 1 An atmospheric water tank in which the water is stored in a water tank of a meter size for a first time at a first water temperature by the first step and then for a second time at a second water temperature by a second step; A stressor installed in a path along which the eel held in the atmospheric water tank travels to apply a stimulus to the skin of the eel for a third time period by at least one of illumination, electric stimulation, and ultrasound; And a circular tunnel part through which one of the eels passed through the stress part comes in contact with the skin of the eel while scraping off secretions from the skin of the eel; . ≪ / RTI >
Wherein the stress portion is provided at an intermediate portion of the path, and the mucin sensor measures the amount of secretion or mucin from the skin of the eel; As shown in FIG.
A high-speed centrifugal separator for separating foreign matter from the circular tunnel portion using a centrifugal separator operated at 1,000 to 15,000 rpm; A mucin concentration unit for collecting the concentrated mucins by separating the mucin in a concentrated state by adding the secretory standard ethanol passed through the high-speed centrifugal separator in the range of 45 to 55 wt%; And a freeze-drying unit for obtaining mucin in a powder state by spraying the concentrated mucin from the mucin concentration unit at a temperature of minus 120 ° C to freeze-dry the mucin. As shown in FIG.
The atmospheric water tank is provided with one or more discharge holes and an inflow hole in the water tank to change or adjust the height of the water in the water tank, and at least one or more holes through which water can pass are formed in the water tank, The stress part is a path of a length of 2 meters in which an eel moves, and a 100-watt incandescent lamp at a distance of 1 meter from the upper part of the path is spaced at intervals of 50 centimeters along the longitudinal direction And an electric stimulation needle and an ultrasonic transducer are installed on the bottom surface, and the circular tunnel portion is a 1-meter-long cylindrical eel tunnel formed in a direction in which the eel moves, and is in direct contact with the circumference of the eel One or more rubber material scratches are repeated 3 to 5 times at uniform intervals along the longitudinal inner surface of the eel tunnel A plurality of holes through which collected secretions are formed may be formed at the bottom of the eel tunnel, and at least one of an electric stimulation needle and an ultrasonic vibrator may be installed.
Wherein the first water temperature is any value selected from the range of 20 to 25 degrees Celsius and the second water temperature is any value selected from the range of 5 to 10 degrees Celsius and the first time is selected from the range of 4 to 6 hours The second time is a value selected from a range of 10 to 20 minutes, and the third time is a value selected from a range of 3 to 10 minutes.
Wherein the first step is to maintain the water height of the water tank in the range of 70 to 80 centimeters when the eel is kept at the first water temperature in the water tank for the first time, The water level of the water tank can be maintained at 30 to 40 centimeters when the eel is kept for the second time.
The present invention having the above-described structure is advantageous in that a large amount of secretions are generated from the skin of the eel and a large amount of eel dermis is collected because stress is applied to the skin of the eel by using illumination, electricity, and ultrasonic waves.
In addition, the present invention having the above-described structure has the effect of reducing the production cost of mucin by reducing the production cost of eels for collecting skin secretions since the eels are collected after collecting secretions from the skin of the eel.
Meanwhile, the present invention having the above-described structure is advantageous in that it can efficiently extract and purify purified mucins using secretions collected from eel.
1 is a flowchart illustrating a method of collecting mucin according to an embodiment of the prior art,
FIG. 2 is a functional block diagram of a system for extracting mucin from eel dermis secretion collected by collecting a large amount of secretions from eel skin according to an embodiment of the present invention,
FIG. 3 is a detailed functional configuration diagram of an atmospheric water tank according to an embodiment of the present invention,
4 is a detailed functional configuration diagram of a stress part according to an embodiment of the present invention,
And
5 is a detailed functional configuration diagram of a circular tunnel according to an embodiment of the present invention.
BRIEF DESCRIPTION OF THE DRAWINGS The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and will be described in detail in the detailed description. It should be understood, however, that the invention is not intended to be limited to the particular embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.
Mucin is a mucin substance secreted from the mucous membranes of living organisms, called mucin or point, difficult to dissolve in water and neutral salt solution, precipitated in acidic, alkaline and viscous liquid phase.
Mucins are largely classified into animal mucins and vegetable mucins, but the following description will be made with respect to animal mucins.
The chemical main body of mucin is a glycoprotein, which contains several glycoproteins depending on the origin of mucus. By way of example, there is a submandibular mucin, but the structure of the pig, It is very different. Other examples include stomach mucin, small intestine mucin, angiomyosin, cervical mucin, and the like. Digestive mucin protects organs and acts as a lubricant for digestive motility, and gastric mucin is used for gastric hyperplasia and gastric ulcer.
In addition, mucin contains chondroitin, a natural complex substance such as collagen, elastin, and protein, so it is said that mucin prevents moisture loss from external harsh environment and prevents drying of skin and quick regeneration of scarred skin.
Thus, mucin is a new substance that gives acne scars, sensitive skin regeneration and elasticity.
FIG. 2 is a functional block diagram of a system for extracting mucin from eel dermis secretion collected and collected by generating a large amount of secretion from eel skin according to an embodiment of the present invention. FIG. 4 is a detailed functional configuration diagram of a stressor according to an embodiment of the present invention. FIG. 5 is a detailed functional configuration diagram of a circular tunnel according to an embodiment of the present invention. FIG. to be.
The
The
The
Experiments were conducted by injecting 50 adult eels into the
The
The material of the
The
Wherein the first water temperature is any one value selected from the range of 20 to 25 degrees Celsius and the second water temperature is any one value selected from the range of 5 to 10 degrees Celsius.
Also, the first time is any value selected from the range of 4 to 6 hours, and the second time is any value selected from the range of 10 to 20 minutes.
On the other hand, the first water temperature is preferably set to 21 degrees Celsius, and it is confirmed by experiments that the eel is stressed when it is set to be less than 20 degrees or more than 26 degrees. It is preferable to set the first time to 5 hours. If it is set to be less than 4 hours, the stabilization ratio in which the eel is adapted to the environment and is gentle is 70% or less, and if it exceeds 6 hours, .
The second water temperature is preferably set at 6 degrees Celsius. If the water temperature is set to less than 5 degrees or more than 11 degrees, it is confirmed that the eel is overcome the stress environment because there is no significant difference in the stress state in which the eel is subjected to stress. It is preferable to set the second time to 12 minutes, and it is confirmed that when the setting is set to less than 10 minutes and the setting time is more than 21 minutes, there is not much change in the stress state of the eel, thereby overcoming the stress environment.
In the first step of stabilizing the eel, 75 centimeters of the water in the water tank is preferable, and when it is less than 70 centimeters, it takes a lot of time to stabilize, and when it exceeds 81 centimeters, there is not a big difference.
Therefore, in the first step, the water temperature, which is the temperature of the water, is set at 21 degrees in the
In the second step of applying the primary stress to the eels, when the water height in the
The eel, which is located inside the
The
On the other hand, the
If the appropriate amount (dose) of secretion or mucin is not detected from the skin (epidermis) of the eel by the control unit in an appropriate capacity available for commercial use or above the first reference level, a stronger stimulation signal So that stronger stress is applied to the skin of the eel so that secretions or mucins are secreted from the skin of the eel.
In addition, the control unit analyzes the data detected by the mucin sensor and determines that secretions or mucins are secreted from the skin of the eel below the second reference level, which is difficult to commercially use from the skin of the eel, And when it is determined that the eels are moving, the travel path switching unit is driven so that the eels are returned to the waiting water tank. Although not shown in the drawing, the movement path switching unit can use or generally adopt a movement path switching structure of an eel.
The eel returning to the atmospheric reservoir returns to the skin through the first and second steps after releasing the secretion to the skin with time, and the amount of secretion or mucin is measured from the mucin sensor.
On the other hand, the above-mentioned 3 hours consisted of any one value selected from the range of 3 minutes to 10 minutes, and it was confirmed that maintaining the 5 minutes gave the greatest amount of stress to the eel and the amount of the secretion was great. It is confirmed that the amount of secretion decreases.
The
The
The
The electric stimulation needle (2300) is a module structure that is protruded by a length of 2-3 millimeters in a unit area of 5 centimeters in width and 5 centimeters in length, and each needle is disposed at intervals of 1 centimeter in the front, back, left and right.
Applying a pulse signal having a frequency of 500 to 1000 hertz to each needle of the electric stimulating
If the frequency of the signal applied to each needle of the
The
In addition, it is preferable to control the stress intensity to gradually weaken within the allowable range from the inlet portion where the eel enters the
The circular tunnel part (3000) is constituted to pass through the adult eel and contact the skin of the eel and scrape off the secretion from the skin of the eel.
The
The
The
Scratches (3200) are scaly and are arranged to maintain an inclination angle of 5 per 100 minutes in the direction of the eel, which contacts the skin of the eel and scrape the secretions from the skin of the eel in a mechanical manner, And is collected by the collecting
The inner bottom surface of the
When one or more of the electric stimulating
The
The eel passed through the
The high-speed
The
That is, the substance precipitated or floated by the
The freeze-
Therefore, there is an advantage of efficiently extracting mucin from the secretions collected from the skin of the eel.
It is quite obvious that the composition of the present invention as described above can be applied to all mollusk animals other than eel.
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art.
100: A system for extracting mucin from eel skin secretions collected from a large amount of secretion from eel skin
1000: atmospheric water tank 1100: water tank
1200: Inlet ball 1300: Outlet ball
2000: Stress part 2100: Incandescent light
2200: ultrasonic transducer 2300: electric stimulation needle
3000: Circular tunnel part 3100: Eel tunnel
3200: Scratch portion 3300: Hole
3400: electric stimulation needle 3500: ultrasonic vibrator
3600: Base plate 3700: Collection tray
4000: High-speed centrifuge unit 5000: Mucin concentration unit
6000: Freeze dryer section
Claims (11)
A stressor installed in a path along which the eel held in the atmospheric water tank travels to apply a stimulus to the skin of the eel for a third time period by at least one of illumination, electric stimulation, and ultrasound; And
A circular tunnel part for passing through one of the eels passing through the stress part and scraping off secretions from the skin of the eel by contacting the skin of the eel; Wherein the mucus is extracted from the eel dermis secretion collected from a large amount of secretion from the eel skin.
Wherein the stress portion is provided at an intermediate portion of the path, and the mucin sensor measures the amount of secretion or mucin from the skin of the eel; Wherein the mucus is extracted from the eel dermis secretion collected from the eel skin to produce a large amount of secretion from the eel skin.
A high speed centrifugal separator for centrifugally separating the secretion collected from the circular tunnel portion to separate foreign matter; Wherein the mucus is extracted from the eel dermis secretion collected from the eel skin to produce a large amount of secretion from the eel skin.
A mucin concentration unit for collecting the concentrated mucin by separating the mucin into a concentrated state by adding ethanol to the secretion passed through the high speed centrifugal separation unit; Wherein the mucus is extracted from the eel dermis secretion collected from the eel skin to produce a large amount of secretion from the eel skin.
A freeze-drying unit for obtaining a mucin in a powder state by spraying the mucin concentrated from the mucin-concentrated portion under a temperature condition of freezing and freeze-drying; Wherein the mucus is extracted from the eel dermis secretion collected from the eel skin to produce a large amount of secretion from the eel skin.
The atmospheric water tank is provided with one or more discharge holes and an inflow hole in the water tank to change or adjust the height of the water in the water tank, and at least one or more holes through which water can pass are formed in the water tank, Wherein the mucus is extracted from the eel dermis secretion collected from the eel skin to produce a large amount of secretion from the eel skin.
Characterized in that the stress portion comprises a structure in which an incandescent lamp or the like is provided along the longitudinal direction at an upper portion of a path through which an eel travels and at least one of an electric stimulation needle and an ultrasonic vibrator is installed on a bottom surface thereof A system for extracting mucin from a large amount of secretion produced and harvested from the skin.
The circular tunnel portion is a cylindrical eel tunnel formed in a direction in which an eel is moved, and one or more scratches directly contacting the eel of the eel are formed in three to five repetitions at uniform intervals along the longitudinal inner surface of the eel tunnel Wherein at least one of at least one of an electric stimulation needle and an ultrasonic vibrator is provided at the bottom of the eel tunnel and at least one or more holes through which the collected secretion is transmitted is formed. And extracts mucin from the collected eel skin secretions.
In the water tank, length values of length * length * height are 1.5 * 0.7 * 1 meter,
The centrifuge operates in the range of 1,000 to 15,000 rpm,
Wherein the freeze-drying unit is driven at a temperature of minus 120 degrees Celsius. A system for extracting mucin from eel dermis secretion collected and harvested by generating a large amount of secretion from eel skin.
Wherein the first water temperature is a value selected from the range of 20 to 25 degrees Celsius,
The second water temperature is any one value selected from the range of 5 to 10 degrees Celsius,
Wherein the first time is a value selected from the range of 4 to 6 hours,
The second time is a value selected from the range of 10 to 20 minutes,
And the third time is a value selected from the range of 3 to 10 minutes. A system for extracting mucin from eel dermis secretion obtained by collecting and collecting a large amount of secretion from eel skin.
The first step
Wherein the water level of the water tank is maintained at 70 to 80 centimeters when the eel is kept in the water tank for the first time from the first water temperature,
The second step
Wherein the water level of the water tank is maintained at 30 to 40 centimeters when the eel is kept in the water tank for the second time from the water tank to the second water temperature. Lt; / RTI >
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CN109601437A (en) * | 2019-01-25 | 2019-04-12 | 淮海工学院 | The method that Mucilage of loach stress is secreted and collected |
WO2021167311A3 (en) * | 2020-02-17 | 2021-10-14 | 이성규 | Enzyme food composition for ameliorating obesity and skin wrinkles, comprising fast-fermented rhynchosia nulubilis paste and eel mucus |
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KR20130101779A (en) | 2012-03-06 | 2013-09-16 | (주)유성글로벌 | Hybrid virtual disk service system |
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KR20130101779A (en) | 2012-03-06 | 2013-09-16 | (주)유성글로벌 | Hybrid virtual disk service system |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109601437A (en) * | 2019-01-25 | 2019-04-12 | 淮海工学院 | The method that Mucilage of loach stress is secreted and collected |
CN109601437B (en) * | 2019-01-25 | 2021-06-15 | 淮海工学院 | Method for stress secretion and collection of loach mucus |
WO2021167311A3 (en) * | 2020-02-17 | 2021-10-14 | 이성규 | Enzyme food composition for ameliorating obesity and skin wrinkles, comprising fast-fermented rhynchosia nulubilis paste and eel mucus |
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