KR20160125131A - Method for increasing tyrosine content in peanut sprout using light emitting diode irradiation - Google Patents

Abstract

The present invention relates to a method for increasing tyrosine content in peanut sprouts in comparison to a control group, a preparation method of peanut sprouts having increased tyrosine content in comparison to a control group, peanut sprouts having increased tyrosine content prepared by the method, and to a processed food containing the peanut sprouts. More particularly, the method for increasing tyrosine content in peanut sprouts in comparison to a control group comprises the following steps of: (a) germinating peanut seeds by soaking the peanut seeds in a dark condition; and (b) radiating a light emitting diode (LED) having a far-red light wavelength to the germinated peanut seeds obtained from the step (a).

Description

FIELD OF THE INVENTION [0001] The present invention relates to a method for increasing the tyrosine content of a peanut sprout using LED irradiation,

(A) germinating the peanut seeds by soaking them in a dark condition; And (b) irradiating germinated peanut seeds of step (a) with a light emitting diode (LED) having a primary red light wavelength. The method for increasing the tyrosine content of peanut sprouts as compared to the control, This invention relates to a process for the production of peanut sprouts, to peanut sprouts with increased tyrosine content produced by said process, and to processed foods containing said peanut sprouts.

As consumers' interest in life has increased with recent improvement in living standards, the tendency to prefer foods with more functionality in the selection and ingestion of foods is becoming stronger. Recently, germplasm has been increasingly consumed as germination plants have been reported to be effective in improving adult diseases.

Although peanuts contain abundant nutrients, they are recognized as nutritionally bad due to high fat and cholesterol content. Recently, due to the importation of low-priced peanuts from China and other countries, the cultivation area of domestic peanuts has reached 9.4 thousand ha (1995 Year) to 3.3 thousand hectares (2007), and the production volume is continuously decreasing from 17.2 thousand tons (1995) to less than 6 thousand tons (2007).

When the peanut grows as a sprout vegetable rather than the seed, it can be confirmed that the content of niacinamide, pyridoxine and thiamine is increased as well as the content of vitamin C which helps iron absorption by reducing antioxidant active enzyme and collagen synthesis and nonheme iron, In particular, it contains an excellent aspartic acid as an alcohololytic enzyme and arginine, methionine and glutamic acid which affect the immunity enhancement.

The LED, invented around 1960, is a semiconductor device that emits light when a voltage is applied in a forward direction by using the characteristics of a compound semiconductor. The luminescent color of the LED can be manufactured to emit light in the ultraviolet region, the visible region and the infrared region depending on the material used. The lifetime of LEDs is considerably longer than that of incandescent bulbs, with lower energy consumption and very few environmentally harmful substances.

In addition, while the conventional illumination has a wide wavelength band region, LEDs are used for the promotion of growth of plants and prevention of pests due to the accurate and very narrow wavelength range. US NASA used LEDs to grow plants in space, and now these LEDs are available in mass production and are used in a variety of fields in agriculture. In Japan, since the 1990s, non-chemical high quality vegetables and flowers have been provided to consumers through LED plants. LEDs are also used for mass production of high-quality crop seedlings, industrial material plant production, and medicinal and medical plant raw materials. In particular, LEDs can provide a stable source of light for 365 days regardless of climate change, for example in the case of plant factories or green house cultivation, which is an example of a typical clean technology.

Korean Patent No. 1238708 discloses a method of increasing the vitamin C of safflower sprouts by LED light source conditions and Korean Patent Laid-Open Publication No. 2014-0123212 discloses a method of increasing the ginsenoside content of ginseng roots using LED irradiation However, this method is different from the method of increasing tyrosine content of peanut sprouts using the LED irradiation of the present invention.

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned needs, and it is an object of the present invention to provide a method for efficiently and eco-friendly LEDs of germinated peanut seeds by irradiating specific wavelengths of LEDs to increase specific amino acid contents such as tyrosine of peanut sprout The present invention has been completed.

In order to solve the above problems, the present invention provides a method for producing peanut seeds, comprising the steps of: (a) germinating peanut seeds by soaking in dark conditions; And (b) irradiating germinated peanut seeds of step (a) with a red light emitting diode (LED). The present invention also provides a method for increasing the tyrosine content of peanut sprouts.

The present invention also provides a method for producing peanut seeds, comprising the steps of: (a) germinating peanut seeds in a dark condition; And (b) irradiating a germinated peanut seed of the step (a) with a red light emitting diode (LED) to the peanut sprout.

The present invention also provides peanut sprouts with increased tyrosine content produced by the method.

The present invention also provides a processed food containing the peanut sprouts.

The present invention is a very useful invention that can improve the food value of peanut sprouts by improving the content of useful amino acids such as tyrosine as compared with existing peanut sprouts, thereby greatly improving the added value in terms of economy.

FIG. 1 is a photograph showing the growth process of peanut buds for 14 days according to light treatment conditions.
FIG. 2 is a photograph showing a comparison of growth patterns of peanut buds according to light treatment conditions.
FIG. 3 is a graph showing the effect of glutamic acid (Glu), serine (Ser), glycine (Gly), histidine (His), cysteine (Cys2), arginine (Arg), alanine (Ala), proline Tyrosine (Tyr) content as a relative value to the control.
The number under the graph in FIG. 3 means the area where the amino acid analyzer measured the amount of amino acid.
1 to 3 Dark: dark condition, White: white light, Blue: blue light, Red: red light, and FR: far red light.

In order to achieve the object of the present invention,

(a) germinating the peanut seeds by soaking in dark conditions; And

(b) irradiating germinated peanut seeds of step (a) with a light emitting diode (LED) having a primary red light wavelength, wherein the amino acid content of the peanut bud is increased compared to the control.

In the method according to an embodiment of the present invention, the term " LED "refers to a light emitting diode, and the LED lighting apparatus emits light of the optimum wavelength A plurality of types of LEDs are combined at an appropriate ratio and a plurality of such LEDs are arranged in a member such as a panel. The LED is a far-red light (730 ± 20 nm) wavelength But is not limited thereto.

The term " irradiation "refers to exposing the plant surface to LED light by illuminating the light on the surface.

In addition, in the method according to an embodiment of the present invention, the control means treating germinated peanut seeds with a dark condition instead of an LED irradiation.

In addition, in the method according to an embodiment of the present invention, the amino acid may be tyrosine, but is not limited thereto.

The method of increasing the tyrosine content of peanut sprouts compared to the control of the present invention is more specifically

(a) germinating the peanut seeds by soaking them in dark conditions at 22 to 28 DEG C for 12 to 16 days; And

(b) irradiating the germinated peanut seed of step (a) with a far-red light emitting diode (LED) at 22 to 28 ° C for 20 to 28 hours,

More specifically,

(a) seeding the peanut seeds by soaking them in dark conditions at 25 占 폚 for 14 days; And

(b) irradiating germinated peanut seeds of step (a) with a far-red light emitting diode (LED) at 25 ° C for 24 hours.

The present invention also relates to

(a) germinating the peanut seeds by soaking them in dark conditions at 22 to 28 DEG C for 12 to 16 days; And

(b) irradiating the germinated peanut seed of step (a) with a red light emitting diode (LED) at 22 to 28 ° C for 20 to 28 hours, wherein the tyrosine content is higher than that of the control Thereby providing a method for manufacturing an increased peanut sprout.

The method for producing peanut sprouts increased in tyrosine content compared to the control of the present invention is more specifically

(a) seeding the peanut seeds by soaking them in dark conditions at 25 占 폚 for 14 days; And

(b) irradiating germinated peanut seeds of step (a) with a far-red light emitting diode (LED) at 25 ° C for 24 hours.

The present invention also provides peanut sprouts with increased tyrosine content produced by the method.

The present invention also provides a processed food containing the peanut sprouts. There is no particular limitation on the kind of the processed food. Examples of the foods to which the peanut sprouts can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, soups, drinks, tea, , An alcoholic beverage and a vitamin complex, and includes all processed foods in a conventional sense.

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Peanut bud LED  process

The peanuts were washed with 70% ethanol, then diluted 50 times with Lax and sterilized for 30 minutes. After incubation at 25 ° C for 14 days in the incubator, the light source was installed at a height of 30 cm and treated with light for 24 hours. The light treatment conditions were 450 nm of blue light, 650 nm of red light, 730 nm of red light, 365 nm of UV-A, 302 nm of UV-B and 254 nm of UV-C, respectively.

Example  One: In light processing  Of peanut bud growth

Peanut buds began to appear on the 6th day after rooting for 14 days in dark condition. On the 10th day, the hypocotyl began to appear outside the cotyledon, and from the 12th day, the height of the upper axis of the hypocotyl and root rapidly progressed (Fig. 1).

The growth of peanut buds during 14 days of light treatment was not different from that of cancer treatment until 4th day, but there was difference in hypocotyl growth and leaf height after 12 days from the hypocotyl growth after 6 days, it started. Changes in the growth of peanut sprouts during 14 days showed that the light treatment had a negative effect on the growth of peanut top and bottom hypocotyls and roots (Fig. 1). Therefore, it was determined that cultivation of peanut buds is the most favorable cultivation condition, and cultivation of peanut buds using light treatment for 1 day after cancer treatment is most preferable (Fig. 2).

Example  2: Amino acid content assay

The contents of glutamic acid (Glu), serine (Gly), histidine (His), arginine (Arg), alanine (Ala) and proline (Pro) Although not shown, the tyrosine (Tyr) content was most significantly enhanced in the case of F-red treatment (FIG. 3).

Claims (6)

(a) germinating the peanut seeds by soaking in dark conditions; And
(b) irradiating germinated peanut seeds of step (a) with a light emitting diode (LED) having a primary red light wavelength, wherein the amino acid content of the peanut bud is increased compared to the control. 2. The method of claim 1, wherein the amino acid is tyrosine. The method according to claim 1,
(a) germinating the peanut seeds by soaking them in dark conditions at 22 to 28 DEG C for 12 to 16 days; And
(b) irradiating the germinated peanut seeds of step (a) with a red light emitting diode (LED) at 22-28 ° C for 20-28 hours, compared to a control comprising peanut sprout tyrosine ) Content. (a) germinating the peanut seeds by soaking them in dark conditions at 22 to 28 DEG C for 12 to 16 days; And
(b) irradiating the germinated peanut seed of step (a) with a red light emitting diode (LED) at 22 to 28 ° C for 20 to 28 hours, wherein the tyrosine content is higher than that of the control A method for manufacturing an increased peanut bud. A peanut bud with increased tyrosine content produced by the method of claim 4. Processed food containing the peanut bud of paragraph 5.

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