KR20160117744A - Microalgae separation apparatus using ultrasonic - Google Patents

Microalgae separation apparatus using ultrasonic Download PDF

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Publication number
KR20160117744A
KR20160117744A KR1020150044805A KR20150044805A KR20160117744A KR 20160117744 A KR20160117744 A KR 20160117744A KR 1020150044805 A KR1020150044805 A KR 1020150044805A KR 20150044805 A KR20150044805 A KR 20150044805A KR 20160117744 A KR20160117744 A KR 20160117744A
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KR
South Korea
Prior art keywords
cuvette
space
main body
microalgae
fluid
Prior art date
Application number
KR1020150044805A
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Korean (ko)
Inventor
정상화
김성복
Original Assignee
조선대학교산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 조선대학교산학협력단 filed Critical 조선대학교산학협력단
Priority to KR1020150044805A priority Critical patent/KR20160117744A/en
Publication of KR20160117744A publication Critical patent/KR20160117744A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

Abstract

The present invention relates to an apparatus for separating microalgae using ultrasonic waves, comprising: a main body having a space portion formed along a longitudinal direction thereof and having an injection portion, an upper discharge portion, and a lower discharge portion communicating with the space portion; A cuvette inserted and mounted in the space, the cuvette having a channel formed therein so as to pass a fluid including microalgae; An ultrasonic wave generator installed on the outside of the cuvette and sending ultrasound waves toward the flow channel so as to aggregate microalgae contained in the fluid passing through the cuvette; A cooling unit provided in the main body to cool the ultrasonic wave generator; A supply pump provided on the injection unit side; And a suction pump installed on the side of the upper discharge portion.
The apparatus for separating microalgae using ultrasonic waves according to the present invention not only efficiently separates microalgae while continuously flowing a fluid but also continuously cooling an ultrasonic wave generator by an air cooling method through a cooling unit to improve the operation reliability of the ultrasonic generator have. In addition, since fine particles in a fluid can be controlled in a large amount during flow, it is possible to separate and purify fine particles, which is advantageous for application to various fields such as cell or DNA control and nanotechnology for bio and medical analysis .

Description

[0001] The present invention relates to a microalgae separation apparatus using ultrasonic waves,

The present invention relates to a micro-algae separation apparatus using ultrasonic waves, and more particularly, to a micro-algae separation apparatus using an ultrasonic wave that can control the movement of micro-algae in a culture liquid by using ultrasonic waves, .

Generally, applying ultrasonic waves to the particles contained in the medium can force the particles due to the acoustic radiation force. These techniques are capable of controlling fine particles having a size of several to several tens of micrometers or less floating along the medium, and thus are being actively applied to the analysis of organic particles such as cells and DNA. Especially, As industrial fields requiring precise control of fine particles are becoming diverse, much attention is being given to them.

The control process of such fine particles is classified into separation, purification, harvesting, trapping, and filtration according to their purpose. For this purpose, electromagnetic, mechanical, and optical methods Various methods are applied.

Conventional fine particle control methods are known such as natural sedimentation using gravity, centrifugal separation using centrifugal force, agglomeration using a flocculant, various filtration methods using a filter, and the like. However, There has been a problem in that the operation must be different according to the physical properties and the operation thereof is also complicated. Therefore, a new method for easily separating the particles regardless of the nature of the particles mixed in the solution is required.

Compared with the above-described conventional techniques, the method using ultrasonic waves has an advantage that a large amount of particles can be controlled at a high speed. Above all, since the size of the acoustic radiation force by ultrasonic waves is related to the mechanical properties of particles, It can be applied irrespective of the electrical and magnetic characteristics. Therefore, micro-algae control technology using ultrasonic waves is very applicable in various industrial fields including bio-engineering and nano-engineering.

A standing wave is a phenomenon in which two waves with the same wavelength are seen facing each other and overlapping when they meet. This wave appears as though waves are staying in place, and the same waves, which generally run in opposite directions, overlap each other .

At this time, there is a point where the sound pressure of the wave is always 0 and a point where the sound pressure shows the maximum / minimum according to time. The former is a sound pressure node and the latter is a sound pressure antinode.

When the particle is located in the space where the standing waves are formed, the acoustic radiation force acts toward the negative pressure or semi-negative pressure node of the standing wave depending on the relative mechanical properties of the particles. Because of the acoustic radiation acting on these particles, the particles move to the negative or semi-negative nodes of the standing wave, so that the position of the fine particles can be controlled by using standing waves.

KR 10-2010-0027390 A KR 10-2014-0076590 A KR 10-1036442 B1

SUMMARY OF THE INVENTION The present invention has been made in order to solve the conventional problems as described above, and it is an object of the present invention to provide a method of controlling a microalgae by applying a standing wave having a sound pressure node to microalgae contained in a fluid and controlling the microalgae to move to a position of a sound- The present invention has been made in view of the above problems, and it is an object of the present invention to provide an apparatus for separating microalgae using a supersonic wave.

In order to accomplish the above object, there is provided an apparatus for separating microalgae using ultrasound according to the present invention, wherein a space portion is formed along a longitudinal direction of the microalgae, and an injection portion, an upper discharge portion, Respectively; A cuvette inserted and mounted in the space, the cuvette having a channel formed therein so as to pass a fluid including microalgae; An ultrasonic wave generator installed on the outside of the cuvette and sending ultrasound waves toward the flow channel so as to aggregate microalgae contained in the fluid passing through the cuvette; A supply pump provided on the injection unit side; And a suction pump installed on the side of the upper discharge portion.

The main body includes an upper body and a lower body which are coupled or detachable to each other in a vertical direction. The upper body is opened at the lower part, the upper part is closed, the space is formed inside, And a lower space portion forming the space portion together with the upper space portion is formed in the lower portion and the lower space portion is formed in a lower portion of the lower portion, And an inflow portion for injecting fluid into the lower space portion is formed on one side of the cuvette, and a fluid introduced into the lower space portion through the infusion portion is introduced between the cuvette and the lower body, A first gasket for supporting a lower portion of the cuvette is provided, Has a shape corresponding to the space portion cross-sectional profile, characterized in that the second gasket is formed with a central through hole installed.

A cooling unit provided in the main body to cool the ultrasonic wave generator; And further comprising:

Wherein the cooling unit includes an air supply unit formed at one side of the main body so as to supply air to the space between the main body and the cuvette and air supplied to the space between the main body and the cuvette through the air supply unit to the outside of the main body And an air discharging portion formed on the other side of the main body to discharge the air.

The apparatus for separating microalgae using ultrasonic waves according to the present invention not only efficiently separates microalgae while continuously flowing a fluid but also continuously cooling an ultrasonic wave generator by an air cooling method through a cooling unit to improve the operation reliability of the ultrasonic generator have.

In addition, it is possible to control fine particles in the fluid in a large amount during flow, so that it is possible to separate and purify the fine particles, and it is possible to provide a method for controlling the environment such as cell or DNA control for bio and medical analysis, nanotechnology, It can be applied to various fields such as pollution measurement.

1 is a perspective view of an apparatus for separating microalgae using ultrasonic waves according to the present invention.
FIG. 2 is an exploded perspective view of the micro-algae separation apparatus using ultrasonic waves shown in FIG. 1. FIG.
3 is a cross-sectional view of the upper body shown in Fig.
4 is a cross-sectional view of the micro-algae separation apparatus using ultrasonic waves shown in Figs. 1 and 2. Fig.
FIG. 5 is a sectional view for explaining a process of separating microalgae using the ultrasonic wave micro-algae separation apparatus shown in FIGS. 1 to 4. FIG.
FIG. 6 is a sectional view for explaining a process of discharging microalgae settled using the ultrasonic wave micro-algae separation apparatus shown in FIGS. 1 to 4. FIG.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, a micro-algae separation apparatus using ultrasonic waves according to a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings.

1 to 6 show an apparatus for separating microalgae using ultrasonic waves according to the present invention. 1 to 6, an apparatus 1 for separating microalgae using ultrasonic waves according to the present invention comprises a body 10; A cuvette 20; An ultrasound generating unit 30; A cooling unit 40; A feed pump 50; And a suction pump (60).

The main body 10 is formed with a space portion along the longitudinal direction and an injection portion 122, an upper discharge portion 112 and a lower discharge portion 123 are formed to communicate with the space portion . The injection unit 122 is connected to the fluid supply pipe so as to extend the fluid to the space side, and the upper discharge unit 112 extends upward to discharge the fluid after the micro- And the lower discharge portion 123 extends downward so as to discharge micro-algae separated from the fluid and settled.

The main body 10 includes an upper body 110 and a lower body 120 formed to be vertically coupled or detachable to each other.

The upper body 110 is opened at its lower portion and closed at its upper portion. An upper cavity portion 111 through which the space portion is formed and a cuvette 20 to be described later is inserted is formed in the upper body 110, (Not shown). The upper body 110 is formed with three openings 115 which extend from the outer circumferential surface toward the upper hollow portion 111.

The lower body 120 has an upper portion opened and a lower portion closed. A lower space portion 121 forming the space portion together with the upper hollow portion 111 is formed in the lower body 120, and the lower discharge portion 123 are formed. An injection part 122 for injecting fluid into the lower space part 121 is formed on one side of the lower body 120.

The cuvette 20 is inserted into the space, and a flow path 21 is formed in the cuvette 20 so as to pass the fluid including the microalgae. The cuvette 20 is made of quartz and is formed into a square column shape. When the cuvette 20 is inserted into the main body 10, the two sidewalls facing each other are in close contact with the inner circumferential surface of the space, and the space of the main body 10 is spaced apart from the center space 20 occupied by the cuvette 20, And a left side space portion and a right side space portion located on both sides facing each other with respect to the center side space portion. The right side space defined by the cuvette 20 is communicated with the outside air by an opening 115 formed in the upper body 110 and can be seen through the opening 115 from the outside.

The ultrasound generating unit 30 is provided outside the cuvette 20 to transmit ultrasonic waves toward the flow path 21 to aggregate microalgae contained in the fluid passing through the cuvette 20 . The ultrasonic generator 30 includes an ultrasonic oscillator 31 for generating ultrasonic waves of a predetermined frequency and a reflection unit 32 for reflecting the ultrasonic waves transmitted from the ultrasonic oscillator 31. Alternatively, the ultrasonic generator 30 may include a pair of ultrasonic oscillators 31 generating the same frequency.

The ultrasonic wave generator 30 is disposed on one side of the four sides of the cuvette 20 facing the left side space and the right side space defined by the cuvette 20 and on the other side.

The cooling unit 40 is provided in the main body 10 to cool the ultrasound generating unit 30 in an air cooling manner and includes a space portion between the main body 10 and the cuvette 20, An air supply unit 41 formed at one side of the main body 10 to supply air to the space and air supplied to a space between the main body 10 and the cuvette 20 through the air supply unit 41 And an air discharging part 42 formed on the other side of the main body 10 so as to discharge the air to the outside of the main body 10.

The air supply part 41 is formed at a position corresponding to the position of the left space part so as to blow air into the left space part.

The air discharging part 42 is formed on the outer side of the upper body 110 and communicates with the left space part so that air flowing into the left space part through the air supplying part 41 flows into the main body 10, And discharged to the outside.

The cooling unit 40 may cool the ultrasonic wave generating unit 30 by blowing air into the space on the left side where the ultrasonic wave generating unit 30 is installed so that the operation failure or efficiency due to deterioration of the ultrasonic wave generating unit 30 It is possible to increase the separation efficiency of microalgae.

The supply pump 50 is installed on the injection unit 122 side to supply fluid from a storage tank for culturing microalgae or a storage tank storing the fluid to the injection unit 122.

The suction pump 60 is installed on the side of the upper discharge unit 112 so that micro vortices are generated at a set position, that is, at a sound pressure level or a semi-sound pressure level, by the acoustic radiation force of the ultrasonic waves generated in the above- So that the fluid which does not contain the microalgae in the precipitated state while being agglomerated is sucked from above and discharged from the main body 10.

The apparatus 1 for separating microalgae using ultrasonic waves according to the present invention is characterized in that a fluid flowing into the lower space part 121 through the injection part 122 is inserted between the cuvette 20 and the lower body 120, A first gasket 70 for guiding the cuvette 20 to flow into the flow path 21 of the cuvette 20 and supporting the lower portion of the cuvette 20, A second gasket 80 having a shape corresponding to the cross-sectional shape of the space portion and a through hole formed at the center thereof.

The first gasket 70 is formed in a disc shape so as to be seated in a mounting groove provided on the upper side of the lower body 120, and a through hole is formed at the center. The through hole formed in the first gasket 70 is formed to be smaller than the size of the flow passage 21 so that the entire lower surface of the cuvette 20 can be brought into contact with the upper surface of the first gasket 70.

The second gasket 80 is in close contact with the inner circumferential surface of the upper portion of the space and blocks the fluid from leaking to the outside of the cuvette 20, not through the flow path 21 of the cuvette 20. One side of the second gasket 80 is formed in the air passage hole at a position corresponding to the air supply unit 41 so that the air supplied from the air supply unit 41 can pass through. The through-holes formed in the second gasket 80 are formed to be smaller in size than the flow path 21 of the cuvette 20.

A process of separating microalgae from a fluid through the micro-algae branching apparatus using ultrasonic waves according to the present invention having the above-described structure will be described with reference to FIGS. 5 and 6. FIG.

First, when the supply pump 50 and the suction pump 60 are operated, the fluid is gradually introduced into the lower space portion 121 through the injection portion 122 by the supply pump 50 and flows into the lower space portion 121 The fluid flows into the flow path 21 of the cuvette 20 through the through hole of the first gasket 70 by the suction force of the suction pump 60 to fill the flow path 21. [

The ultrasonic wave generating unit 30 sends the ultrasonic waves toward the cuvette 20 while the fluid is filled in the flow path 21 of the cuvette 20 and the ultrasonic waves oscillated from the ultrasonic oscillator and the ultrasonic waves reflected from the reflecting unit 32 Standing waves occur as they overlap each other. In this standing wave, a sound pressure node having a sound pressure of zero and a sound pressure antinode indicating a maximum / minimum sound pressure according to time are displayed. As shown in FIG. 5, a position corresponding to the sound pressure node and the semi-sound pressure node The microalgae flocculate.

The microalgae agglomerated at the positions of the sound pressure of the standing wave formed by the ultrasonic oscillator and the reflection part 32 and the semi-sound pressure node are gradually deposited in the lower space part 121 with increasing weight, The micro-algae are removed from the fluid staying on the filter 21.

The micro-algae are discharged to the outside of the main body 10 through the upper discharge port by the suction force of the suction pump 60 and the micro-algae settled in the lower space part 121 are discharged to the main body 10 ), Thereby separating the fluid and the microalgae.

The process of separating the microalgae through the apparatus 1 for separating microalgae using the ultrasonic wave according to the present invention is performed continuously and the discharge of the fluid in the state of supplying the fluid and the microalgae and the discharge of the microalgae .

This process is performed by adjusting the flow rate of the fluid flowing into the lower space portion 121 by the supply pump 50 and the flow rate of the fluid discharged to the upper discharge portion 112 by the suction pump 60 .

During the operation of the ultrasonic generator 30, air is continuously introduced into the left space through the air supply unit 41. The introduced air absorbs the heat generated by the ultrasonic oscillator 31 and is discharged to the air outlet .

The micro-algae separating apparatus 1 using the ultrasonic wave according to the present invention as described above can continuously and easily separate the micro-algae from the fluid by using the ultrasonic waves. The ultrasonic wave generating unit 30, So that the operation reliability of the ultrasonic wave generator 30 can be improved.

While the present invention has been described with reference to exemplary embodiments thereof, it will be understood by those of ordinary skill in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. It will be appreciated that other embodiments are possible.

Therefore, the scope of the true technical protection of the present invention should be determined by the technical idea of the appended claims.

10: Body
110: upper body
120: Lower body
20: Cuvette
30: Ultrasonic wave generator
31: Ultrasonic generator
32:
40:
41:
42:
50: Feed pump
60: Suction pump
70: First gasket
80: second gasket

Claims (4)

A main body in which a space portion is formed along a longitudinal direction and an injection portion, an upper discharge portion and a lower discharge portion are formed so as to communicate with the space portion;
A cuvette inserted and mounted in the space, the cuvette having a channel formed therein so as to pass a fluid including microalgae;
An ultrasonic wave generator installed on the outside of the cuvette and sending ultrasound waves toward the flow channel so as to aggregate microalgae contained in the fluid passing through the cuvette;
A supply pump provided on the injection unit side;
And a suction pump installed on the side of the upper discharge unit. The method according to claim 1,
The main body includes an upper body and a lower body which are formed so as to be coupled to each other in a vertical direction,
Wherein the upper body is opened and the upper portion is closed, an upper space portion in which the space portion is formed and the cuvette is inserted and formed, and the upper discharge portion is formed on the upper side,
The upper body is opened and the lower part is closed. A lower space part forming the space part together with the upper space part is formed inside, a lower discharge part is formed on the lower side, a fluid is injected into the lower space part on one side, An injection section for forming the injection port is formed,
A first gasket is provided between the cuvette and the lower body to guide the fluid introduced into the lower space through the injection unit into the flow path of the cuvette while supporting a lower portion of the cuvette,
Wherein a second gasket is formed between the cuvette and the upper body, the second gasket having a shape corresponding to the cross-sectional shape of the space and having a through hole at the center thereof. The method according to claim 1,
And a cooling unit provided in the main body for cooling the ultrasonic wave generating unit. The method of claim 3,
The cooling unit
An air supply unit formed at one side of the main body so as to supply air to a space between the main body and the cuvette and an air supply unit for discharging air supplied to the space between the main body and the cuvette to the outside of the main body through the air supply unit And an air outlet formed on the other side of the main body.
KR1020150044805A 2015-03-31 2015-03-31 Microalgae separation apparatus using ultrasonic KR20160117744A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200182751A1 (en) * 2018-12-11 2020-06-11 Hitachi, Ltd. Analysis Sample Pretreatment Apparatus, Analysis Sample Pretreatment Method, and Analysis Sample Pretreatment System
CN111812121A (en) * 2020-06-29 2020-10-23 中国人民解放军军事科学院军事医学研究院 Multi-path high-power microwave composite biological irradiation system
KR20220011040A (en) * 2020-07-20 2022-01-27 한남대학교 산학협력단 Green algae removal device and flotation device

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100027390A (en) 2008-09-02 2010-03-11 삼성전자주식회사 Microfluidic cartridge for target molecule purification, molecule purification apparatus using the same, and molecule purification purification method
KR101036442B1 (en) 2009-04-06 2011-05-24 한국표준과학연구원 apparatus and method for collecting contaminated particles by using sonic waves
KR20140076590A (en) 2011-09-28 2014-06-20 어쿠솔트 에이비 System and method to separate cells and/or particles

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100027390A (en) 2008-09-02 2010-03-11 삼성전자주식회사 Microfluidic cartridge for target molecule purification, molecule purification apparatus using the same, and molecule purification purification method
KR101036442B1 (en) 2009-04-06 2011-05-24 한국표준과학연구원 apparatus and method for collecting contaminated particles by using sonic waves
KR20140076590A (en) 2011-09-28 2014-06-20 어쿠솔트 에이비 System and method to separate cells and/or particles

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200182751A1 (en) * 2018-12-11 2020-06-11 Hitachi, Ltd. Analysis Sample Pretreatment Apparatus, Analysis Sample Pretreatment Method, and Analysis Sample Pretreatment System
US11566978B2 (en) * 2018-12-11 2023-01-31 Hitachi, Ltd. System providing an ultrasonic pretreatment for separating particles from an analysis sample
CN111812121A (en) * 2020-06-29 2020-10-23 中国人民解放军军事科学院军事医学研究院 Multi-path high-power microwave composite biological irradiation system
KR20220011040A (en) * 2020-07-20 2022-01-27 한남대학교 산학협력단 Green algae removal device and flotation device

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