KR20160116307A - Cell extracting decive and cell extracting method - Google Patents

Abstract

The present invention relates to an apparatus and a method of extracting cells. More specifically, the apparatus comprises: a washing unit washing tissues; a separation unit rotating the washed tissues and separating cells; an additive providing unit providing a predetermined amount of an additive to the tissues; and a transportation module transporting the tissues, the extracted cells and separated residues. The separation unit comprises a centrifuge. The centrifuge performs the steps of: washing cells with a cell extraction apparatus and washing liquid and transporting the same to the separation unit, wherein the cell extraction apparatus has a cutter in a container to break the tissues during centrifugation; performing separation on the tissues by using the separation unit; and discharging cells and residues separated from the tissues. The separation step comprises a first separation step. The first separation step comprises a first centrifugation step driving the centrifuge for a predetermined time to enable the same to be driven at a predetermined speed; and a first gravity separation step of stopping the centrifuge for a predetermined time to perform a gravity separation process.

Description

[0001] The present invention relates to a cell extracting apparatus and a cell extracting method,

The present invention relates to a cell extracting apparatus and a cell extracting method, and more particularly, to a cell extracting apparatus and a cell extracting method. A separator for separating the cells by rotating the washed tissue; An additive providing unit for providing a predetermined additive to the tissue; And a delivery module for delivering the tissue, the extracted cells, and the separated remainder, wherein the separator comprises a centrifuge, wherein the centrifuge separates the tissue from the vessel, A cell extractor configured to incorporate a cutter, and using the wash liquor to clean and transfer tissue to a separator; Performing separation of the tissue using the separator; Wherein the separating step includes a first separating step in which the centrifuge is operated for a predetermined period of time to remove cells and residues separated from the tissue at a predetermined rate And a first gravity separation step of performing gravity separation by stopping the centrifuge for a predetermined period of time.

BACKGROUND ART [0002] Along with the development of medical technology, a lot of operations and procedures have been developed for separating tissues from one part of the body and extracting predetermined cells from tissues for reconstruction and transplantation of lost body tissues, beauty or the like. For this purpose, predetermined extraction devices and methods are used to separate specific cells, substances, etc. from a predetermined tissue such as adipose tissue.

Such cell extracting apparatuses and methods are usually carried out through washing of tissue, extraction of tissue, mixing with predetermined enzyme addition, centrifugation, and washing.

However, a large amount of cells are lost in the process of using the extracting apparatus and extracting method, resulting in a very small amount of cells to be obtained. In addition, since the size of cells formed in the tissue is not uniform, aggregation may occur in the extraction process, resulting in deterioration of extraction efficiency and degradation of quality of extracted products.

Therefore, it is necessary to develop a cell extracting apparatus and a method capable of overcoming such difficulties, improving cell extraction efficiency, and improving the quality of the extraction result.

Patent No. 10-1127015

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a cell extracting apparatus for extracting cells in a tissue. A separator for separating the cells by rotating the washed tissue; An additive providing unit for providing a predetermined additive to the tissue; And a delivery module for delivering the tissue, the extracted cells, and the separated remainder, wherein the separator comprises a centrifuge, wherein the centrifuge separates the tissue from the vessel, And to provide a cell extracting apparatus having a built-in cutter.

In order to achieve the above object, a cell extracting apparatus according to the present invention is a cell extracting apparatus for extracting cells in a tissue, comprising: a washing unit for washing tissue; A separator for separating the cells by rotating the washed tissue; An additive providing unit for providing a predetermined additive to the tissue; And a delivery module for delivering the tissue, the extracted cells, and the separated remainder, wherein the separator comprises a centrifuge, wherein the centrifuge separates the tissue from the vessel, And a cutter is built therein.

Preferably, the additive comprises an active stabilizer, wherein the active stabilizer comprises at least one of albumin, elastin, PRP, Human serum, Cell Culture media, Cell Conditioned Media, Bovine surum, and hyaluronic acid.

The method for extracting cells in a tissue using the cell extracting apparatus according to the present invention comprises the steps of washing the tissue using a washing solution and moving the tissue to a separating section; Performing separation of tissue using the separator; Wherein the separating step includes a first separating step in which the centrifuge is operated for a predetermined period of time to remove cells and residues separated from the tissue at a predetermined rate And a first gravity separation step of performing gravity separation by stopping the centrifuge for a predetermined time.

Preferably, the first centrifugal separation step is repeatedly performed with a predetermined period of time in which the centrifugal separator is operated and stopped. In the first gravity separation step, the centrifugal separator is stopped for a predetermined period of time and gravity separation is performed Wherein the first centrifugal separation step is repeated for 1 minute to 2 minutes while the centrifuge is rotated for 3 seconds to 5 seconds at a speed of 500 rpm and then for 3 seconds to 5 seconds for a period of 1 minute to 2 minutes, In the separation step, the centrifuge is stopped for 3 minutes to 5 minutes.

Preferably, the discharging step includes a first discharging step of transferring the separated washing liquid to the collection device after the first separation step.

Preferably, an additive administration step of administering the additive in the centrifugal separator after the first discharge step; Wherein the additive comprises an active stabilizer, wherein the active stabilizer comprises at least one of albumin, elastin, PRP, Human serum, Cell Culture media, Cell Conditioned Media, Bovine surum, and hyaluronic acid.

Preferably, the separation step includes a second separation step, wherein the second separation step comprises a second centrifugation step of rotating the centrifuge dispensed with the additive for a predetermined period of time and rotating at a predetermined speed, and And a second gravity separation step of performing gravity separation by stopping the centrifuge for a predetermined time.

Preferably, the second centrifugal separation step is repeatedly performed with a predetermined period of time in which the centrifugal separator is operated and stopped. In the second gravity separation step, the centrifugal separator is stopped for a predetermined time, Wherein the second centrifugal separation step comprises the steps of rotating the centrifuge at a speed of 500 rpm for 3 seconds to 5 seconds and then stopping for 3 seconds to 5 seconds for 15 minutes to 20 minutes, And a second centrifugation step wherein the centrifuge rotates at a speed of 1000 rpm to 3000 rpm for 3 minutes to 5 minutes, and wherein the second gravity separation step comprises the step of centrifuging the centrifuge for 5 to 10 minutes do.

In the cell extracting apparatus according to the present invention, the centrifugal separator has a predetermined cutter, so that the cells are prevented from being clustered, and consequently, the tube and the like, in which the tissue and the extracted cells are moved, can be prevented from being closed.

In addition, when the tissue is divided and the surface area is enlarged due to its small volume, when it is desired to separate and react with various enzymes by using a predetermined enzyme or the like, the reaction efficiency is improved due to the enlarged surface area and the reaction time is shortened, Separation can be achieved.

Further, as a certain active stabilizer is added to tissues during the cell extraction process, cell stabilization and activation of degradation promotion are promoted during cell separation, and thus, rapid isolation, degradation and cell extraction can be achieved.

The cell extracting method according to the present invention comprises a first separating step, wherein the first separating step includes a first centrifuging step of repeating rotating and stopping at a low speed, thereby causing a large damage to the cells in the initial stage It is possible to separate foreign matter which is relatively easy to separate and avoids giving. That is, it is possible to achieve cell extraction while avoiding the loss of the cells to be extracted as much as possible by separating the foreign substance which can be sufficiently separated by centrifugation at a low speed at an early stage.

In addition, as the active stabilizer is added to tissues during the cell extraction process, the cell extraction method according to the present invention facilitates cell stabilization and activation of degradation promoting activity during cell separation, and thus, rapid isolation, degradation, and cell extraction can be achieved.

The separating step further includes a second separating step, wherein the second separating step includes a second centrifuging step, and the second centrifuging step is repeatedly performed while the centrifuge is operated and stopped repeatedly . However, as the low-speed rotation and the medium-speed rotation are performed separately, the loss of the cell to be extracted is minimized and a large amount of cells can be obtained.

1 is a conceptual diagram illustrating a structure of a cell extracting apparatus according to an embodiment of the present invention.
2 is an illustration of a centrifuge used in a cell extraction device according to an embodiment of the present invention.
Figure 3 is a cross-sectional view of the centrifugal separator of Figure 2;
FIGS. 4 and 5 are views showing a partition wall of the centrifugal separator of FIG. 2. FIG.
6 is an enlarged view of K in Fig.
FIGS. 7 to 10 are views showing steps of a cell extracting method according to an embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. The present embodiments are not intended to be limiting.

BRIEF DESCRIPTION OF THE DRAWINGS The advantages and features of the present invention, and the manner of achieving them, will be apparent from and elucidated with reference to the embodiments described hereinafter in conjunction with the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. To fully disclose the scope of the invention to those skilled in the art, and the invention is only defined by the scope of the claims. Like reference numerals refer to like elements throughout the specification.

The terms spatially relative, "below", "beneath", "lower", "above", "upper" May be used to readily describe an element or component and other element or components of the correlation. Spatially relative terms should be understood to include, in addition to the orientation shown in the drawings, terms that include different orientations of members during use or operation. For example, "upper" can be interpreted as "lower" when a member shown in the drawing is inverted. The members can also be oriented in different directions, so that spatially relative terms can be interpreted according to orientation.

The terminology used herein is for the purpose of illustrating embodiments and is not intended to be limiting of the present invention. In the present specification, the singular form includes plural forms unless otherwise specified in the specification. &Quot; comprises, "and / or" comprising ", as used herein, unless the recited element, step, operation and / Or additions.

Unless defined otherwise, all terms (including technical and scientific terms) used herein may be used in a sense commonly understood by one of ordinary skill in the art to which this invention belongs. Also, commonly used predefined terms are not ideally or excessively interpreted unless explicitly defined otherwise.

FIG. 1 is a conceptual diagram showing the structure of a cell extracting apparatus 1 according to an embodiment of the present invention. FIG. 2 is a schematic view of a centrifugal separator 210 used in a cell extracting apparatus 1 according to an embodiment of the present invention. FIG. 3 is a cross-sectional view of the centrifugal separator 210 of FIG. 2, FIGS. 4 and 5 are views showing partition walls of the centrifugal separator 210 of FIG. 2, Fig.

A cell extracting apparatus (1) according to the present invention is a cell extracting apparatus (1) for extracting cells in a tissue, comprising: a washing unit (100) for washing tissue; A separation unit 200 for separating the cells by rotating the washed tissue; An additive preparation part (300) for providing a predetermined additive to the tissue; And a transport module 400 for transporting the tissue, the extracted cells, and the separated residues, wherein the separator 200 includes a centrifuge 210 and the centrifuge 210 , And a cutter is built in the container so as to perform the crushing of the tissue during centrifugation.

The cleaning portion 100 is configured to perform cleaning of the obtained tissue. The cleaning unit 100 may perform cleaning of tissue using a predetermined cleaning liquid. At this time, the tissue may be, for example, a predetermined biological tissue isolated from the body, but is not limited thereto.

The separation unit 200 is provided so that the tissues cleaned through the cleaning unit 100 are collected and collected, and the cells desired to be extracted through the rotation of the tissues can be separated and extracted from the tissues. At this time, the cell to be extracted may be, for example, an adipocyte, but is not limited thereto.

In order to achieve the above object, the separation unit 200 may include a predetermined centrifugal separator 210. The centrifuge 210 may accommodate the tissue in a predetermined container and then apply rotation to separate the components within the tissue.

2 is a view showing an example of a centrifugal separator 210 used in the cell extracting apparatus 1 according to the present invention. The centrifugal separator 210 shown in FIGS. 2 to 6 may be constituted of a so-called centrifugal bowl in which the tissue is received and cells in the tissue are separated by a predetermined rotational force.

Referring to FIG. 2, the centrifugal separator 210 used in the cell extracting apparatus 1 according to the present invention includes a container 30 having a receptacle in which a tissue is received and rotated according to application of a rotational force; Wherein the receiving space is divided into a radially inward space (32) and an outer space (34), the space being spaced a predetermined distance from the bottom surface of the container (30) (40) formed with a gap capable of flowing between the partition walls (34); And a tube portion (50) disposed in the inner space (32) and through which tissue enters and exits; (40) is provided with a depression (38) formed at the center of the bottom surface of the container (30), and a plurality of And the cutters 44 are spaced apart from each other along the circumferential direction of the partition wall 40 by a predetermined distance.

The container (30) is constituted by a barrel having a receiving portion composed of a predetermined receiving space, and is configured to be rotatable according to the application of rotational force. The receiving part may be provided with a predetermined tissue for centrifugation, and may be filled with a predetermined cell tissue such as fat and a material such as water. A predetermined lid 36 may be disposed on the upper portion of the container 30 so as to cover the receptacle formed in the container 30. The lid 36 is provided with connecting means for applying the rotation described above But not limited to,

Preferably, a depression 38 is formed at the center of the bottom surface of the container 30, through which a tissue injected through the tube 50 is adhered. The depressed portion 38 is configured to be depressed downwardly as compared with the bottom surface of the container 30, and can be configured so that separated tissues are piled up by centrifugal separation.

The partition wall 40 is disposed in the receiving portion. As shown in FIG. 4, the partition wall 40 includes a cylindrical body 210. As the partition wall 40 is disposed in the accommodating portion, the accommodating portion is located in the partition wall 40, And an outer space 34 formed on the outer side of the partition wall 40 and radially outward of the partition wall 40.

A gap having a predetermined gap H may be formed between the lower portion of the partition wall 40 and the bottom surface of the container 30 so that the tissue can move along the centrifugal separation. That is, the lower portion of the partition wall 40 may be spaced apart from the bottom surface of the container 30 by a predetermined distance so that the tissue flows through the gap.

A tube portion (50) is disposed in the inner space (32). The tube portion 50 is composed of a predetermined tube extending in the vertical direction, and the upper portion of the tube portion 50 is connected to a predetermined access portion 60 so that tissues and water can be inserted and removed.

A cutter 44 may be provided at a lower portion of the partition 40 to cut and divide the tissue.

After the tissue is injected through the tubular portion 50, when the container 30 is rotated, the tissue is subjected to centrifugal force and pushed outward. At this time, as described above, the bottom surface of the container 30 and the lower portion of the partition wall 40 are spaced from each other and a gap having a predetermined gap H is formed, so that the structure and various materials contained in the container 30 And is discharged into the outer space (34) divided through the partition (40). At this time, the tissue and various materials are pushed to the upper part of the outer space 34 according to the rotation, and are separated from each other according to various peculiar factors such as weight. On the other hand, when centrifugal separation is completed and the rotation of the container 30 is stopped, the tissue poured into the outer space 34 drops and is accumulated in the depressed portion 38 of the bottom surface of the container 30, And the separation of various substances in the tissue can be performed.

That is, in the centrifugal separation process, the tissue moves between the inner space 32 and the outer space 34 through the gap, so that the plurality of cutters 44 provided at the lower portion of the partition wall 40 passes do. Cutting and dividing of the tissue can be accomplished as it passes through the cutter 44, thereby preventing the tissue from becoming too large to form a mass, and the tissue can be easily extracted through the tube 50.

The centrifugal separator 210 of the cell extracting apparatus 1 according to the present invention is provided with a cutter 44 having the above cutter 44 to prevent the aggregation phenomenon as described above, Closure can be prevented.

In addition, when the tissue is divided and the surface area is enlarged due to its small volume, when it is desired to separate and react with various enzymes by using a predetermined enzyme or the like, the reaction efficiency is improved due to the enlarged surface area and the reaction time is shortened, Separation can be achieved.

In addition, in the case of the cell extracting apparatus 1 using the centrifugal separator 210 having such a configuration, as the operation efficiency of the centrifugal separator 210 is increased as described above, the operating efficiency of the cell extracting apparatus 1, Cell extraction efficiency can be improved.

The cutter 44 may be a beam connected to a lower portion of the partition wall 40 and extending outwardly of the partition wall 40. That is, each of the cutters 44 may have a structure such as a predetermined rod or beam connected to the partition wall 40 and extending in a radially outward direction. Accordingly, the plurality of cutters 44 may have a radial structure about the center of the partition wall 40, and the number and spacing thereof are not limited.

On the other hand, a stopper 34 may be disposed on the inner surface of the container 30 in at least one of the spaces between the cutters 44. The stopper 34 contacts the cutter 44 and is configured as a predetermined engaging portion so that the container 30 and the partition 40 can rotate together. Accordingly, the cutter 44 provided in the partition wall 40 also rotates together to cut the tissue flowing through the gap under the partition wall 40.

Preferably, the cutter 44 is configured to have a blade 230 formed on at least one side thereof to perform the division of the tissue.

That is, as shown in FIG. 6, the cutter 44 is relatively sharply polished so as to achieve the division of the tissue, so that the blade 230 is configured to have a sharp end . Accordingly, the division of the tissue passing through the cutter 44 can be achieved more easily. 9, the blade 46 is formed on one side of the cutter 44, but the blade 46 can be formed on any portion of the cutter 44, May have any form.

Meanwhile, the additive supplier 300 may be provided to provide a predetermined additive to the tissue collected in the separator 200.

The additive supplier 300 may be connected to the separator 200 through a predetermined conduit to provide a predetermined additive to the separator 200 as needed. The additive supplier 300 may include a filling container filled with a predetermined additive and a predetermined injection device for injecting the filled additive to the outside. At this time, the kind of the filler to be filled is not limited and may be an enzyme. Preferably, the additive includes an activity stabilizer, and the activity stabilizer is albumin, elastin, PRP, Human serum, Cell Culture media, Cell Conditioned Media, Bovine surum, and hyaluronic acid.

As the active stabilizer is added to tissues during the cell extraction process, activation of cell stabilization and degradation is promoted during cell separation, and thus rapid isolation, degradation and cell extraction can be achieved.

Meanwhile, a transport module 400 for carrying the tissue, the extracted cells, and the separated residues may be provided. The transport module 400 collectively refers to all structures and members capable of carrying the tissue, the extracted cells, and the separated residues, and includes, for example, a predetermined conduit, and a pump capable of transporting the material through a conduit Such as a < / RTI >

Hereinafter, a cell extracting method according to the present invention will be described with reference to FIGS. 7 to 10. FIG.

The cell extracting method according to the present invention comprises the steps of washing a tissue using a washing solution and moving the tissue to a separating part 200; Performing separation of tissue using the separation unit (200); Wherein the separating step comprises a first separating step, wherein the first separating step comprises moving the centrifuge 210 for a predetermined period of time to remove the cells and the residue from the tissue, , And a first gravity separation step for performing gravity separation by stopping the centrifuge 210 for a predetermined time.

First, the obtained tissue is washed. At this time, the tissue is cleaned by the cleaning unit 100, and the cleaning liquid and the tissue can move to the separation unit 200 as shown in FIG. 7 (A).

Then, the separating unit 200 is used to separate the tissue. The separation unit 200 can separate predetermined cells from the tissue through centrifugal separation using the centrifugal separator 210 described above. At this time, since the centrifugal separator 210 includes a predetermined cutter, the cell extracting method according to the present invention can further improve cell extraction efficiency.

The separated cells and the residue are separated and discharged through the separator 200. For example, when the fat cells are to be extracted, the extracted fat cells are collected and stored in a predetermined collection unit 600, and the separated residues may be moved to the rejection unit 500 and discarded.

Meanwhile, a predetermined transport module 400 may be provided for transporting the tissue, cells, and separated residues, and the transport module 400 may include a predetermined pump 410, a valve 420, . At this time, the pump 410 may be a solenoid pump, a peristaltic pump, or the like, but is not limited thereto.

Advantageously, said separating step may comprise a plurality of steps.

First, the separating step includes a first separating step, wherein the first separating step includes a first centrifuging step of rotating the centrifugal separator 210 for a predetermined time to rotate at a predetermined speed, 210) for a predetermined period of time to perform gravity separation.

In this case, preferably, the first centrifugal separation step is repeatedly performed with a predetermined period of time in which the centrifugal separator 210 is operated and stopped. In the first gravity separation step, So that gravity separation can be performed.

In the first centrifugal separation step, the centrifuge 210 rotates at a speed of 500 rpm for 3 seconds to 5 seconds and then stops for 3 seconds to 5 seconds. The rotation and the stop are alternately repeated for 1 minute to 2 minutes, The first gravity separation step may include the step of stopping the centrifuge 210 for 3 to 5 minutes.

As the first centrifugation step is repeatedly rotated and stopped at a low speed, it is possible to prevent foreign matter from being damaged in the initial stage and to separate foreign matter that is relatively easy to separate. That is, it is possible to achieve cell extraction while avoiding the loss of the cells to be extracted as much as possible by separating the foreign substance which can be sufficiently separated by centrifugation at a low speed at an early stage.

Preferably, the discharging step includes a first discharging step of transferring the separated washing liquid to the collection device after the first separation step. That is, as shown in FIG. 8 (B), after the first centrifugal separation step and the first gravity separation step are performed according to the first separation step, the cleaning liquid separated from the tissue can be moved to the collection device and discarded.

Preferably, as shown in FIG. 9C, an additive administration step of administering an additive into the centrifugal separator 210 may be performed, and the additive administration step may be performed after the first discharge step. The active agent may include at least one of albumin, elastin, PRP, human serum, cell culture media, cell conditioned media, bovine serum, and hyaluronic acid.

As the active stabilizer is added to tissues during the cell extraction process, activation of cell stabilization and degradation is promoted during cell separation, and thus rapid isolation, degradation and cell extraction can be achieved.

Preferably, the separating step includes a second separating step, and the second separating step includes a second centrifugal separation step of rotating the centrifugal separator 210 to which the additive has been administered for a predetermined period of time to rotate at a predetermined speed, And a second gravity separation step of performing gravity separation by stopping the centrifuge 210 for a predetermined period of time.

Preferably, the second centrifugation step is repeatedly performed with the predetermined period of time in which the centrifuge 210 is operated and stopped, and the centrifuge 210 rotates at a speed of 500 rpm for 3 seconds to 5 seconds And then the rotation and stopping for 3 to 5 seconds to be repeated are repeated for 15 to 20 minutes and the centrifugal separator 210 is rotated for 3 to 5 minutes at a speed of 1000 rpm to 3000 rpm And a tea centrifugation step.

As described above, since the low-speed rotation and the medium-speed rotation are applied differently, the loss of the cell to be extracted is minimized and a large amount of cells can be obtained.

Meanwhile, in the second gravity separation step, the centrifugal separator 210 stops for a predetermined time to perform gravity separation, and preferably the centrifugal separator 210 can stop for 5 minutes to 10 minutes.

The cells separated through the separation step and the additive administration step as described above may be collected into a predetermined collection unit 600 as shown in FIG. 10D. The transport of the cells may be performed by the transport module 400 described above.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, It should be understood that various modifications may be made by those skilled in the art without departing from the spirit and scope of the invention.

1: cell extractor
20: Centrifuge
30: container
32: My space
34: Outside space
36: Cover
38: depression
40:
42: Body
44: Cutter
50: tube
60: Access
100:
200:
210: Centrifuge
300: additive preparation
400: Transport module
410: pump
420: Valve
500: Rejected
600: collecting section

Claims (5)

A cell extracting apparatus for extracting cells in a tissue,
A cleaning unit for performing cleaning of tissue;
A separator for separating the cells by centrifuging the washed tissue;
An additive providing unit for providing a predetermined additive to the tissue;
A transport module for carrying the tissue, the extracted cells, and an isolated residue;
Rejection of substances that are separated from the tissue and are discarded; And
And a collecting part for collecting and storing the extracted cells,
Wherein the separator includes a centrifugal separator,
Wherein the centrifugal separator is constructed such that a tissue is accommodated and a cutter is embedded in a rotating container so that the tissue is crushed during centrifugation,
The container has a receiving portion in which the tissue is received and is rotated in response to application of a rotational force,
In the centrifugal separator,
A partition wall which is disposed in the receiving portion and is divided into a radially inner space and an outer space, the partition being spaced from the bottom surface of the container by a predetermined distance so that the tissue can flow between the inner space and the outer space; And
A tube portion disposed in the inner space and through which the tissue enters and exits; Wherein a plurality of cutters are provided at a bottom of the bottom wall of the container so as to divide the tissue flowing through the gap at a lower portion of the partition wall, Are spaced apart from each other at a predetermined interval along the circumferential direction of the partition wall. The method according to claim 1,
Wherein the additive comprises an active stabilizer,
The active-
Wherein the cell extracting apparatus comprises at least one of albumin, elastin, PRP, Human serum, Cell Culture media, Cell Conditioned Media, Bovine surum, and hyaluronic acid. The method according to claim 1,
Wherein the transport module comprises:
A conduit for connecting the washing unit, the separation unit, the additive supply unit, the rejection unit, and the collecting unit,
A valve provided in the conduit and connected to the washing unit, the additive supply unit, the rejection unit, and the collecting unit,
A cell extraction device comprising a pump. A cleaning unit for performing cleaning of tissue;
A separator for separating the cells by centrifuging the washed tissue;
An additive providing unit for providing a predetermined additive to the tissue;
A transport module for carrying the tissue, the extracted cells, and an isolated residue;
Rejection of substances that are separated from the tissue and are discarded; And
And a collecting part for collecting and storing the extracted cells,
Wherein the separator includes a centrifugal separator,
In the centrifugal separator,
Wherein the tissue is accommodated and a cutter is built in the rotating container so that the tissue is broken during centrifugation,
Wherein the transport module comprises:
A pipe connecting the washing unit, the separation unit, the additive supply unit, the rejection unit, and the collecting unit, and a valve provided in the channel and connected to the washing unit, the additive supply unit, the rejection unit, A method for extracting cells in a tissue using a cell extracting apparatus comprising:
Washing the tissue filled in the cleaning part with a cleaning solution;
Moving the tissue in the cleaning unit and the cleaning liquid to the separation unit through a channel between the cleaning unit and the separation unit;
A first separating step of separating the tissue and the washing liquid using the separating unit;
A discharging step of discharging the washing liquid separated from the tissue in the separating unit through the channel between the separating unit and the rejection unit to the rejection unit;
An additive administration step of administering the additive from the additive providing portion into the separation portion through a channel between the additive providing portion and the separation portion;
And a second separation step of separating the tissue to which the additive is administered by using the separation section,
Wherein the first separation step comprises a first centrifugation step and a first gravity separation step,
Wherein the second separation step comprises a second centrifugation step and a second gravity separation step,
Wherein the second centrifugation step comprises a primary centrifugation step and a secondary centrifugation step,
Wherein the first centrifugal separation step comprises:
The centrifuge is rotated for 3 to 5 seconds at a speed of 500 rpm and then for 3 to 5 seconds, and the rotation and stopping are repeated for 1 to 2 minutes,
Wherein the first gravity separation step comprises:
The centrifuge is stopped for 3 to 5 minutes to perform gravity separation,
Wherein the primary centrifugation step comprises:
The centrifuge is rotated for 3 to 5 seconds at a speed of 500 rpm and then for 3 to 5 seconds for rotation and stopping for 15 to 20 minutes,
Wherein the secondary centrifugation step comprises:
The centrifuge is allowed to rotate for 3 to 5 minutes at a speed of 1000 to 3000 rpm,
Wherein the second gravity separation step comprises:
Wherein the centrifugal separator is stopped for 5 to 10 minutes to perform gravity separation. 5. The method of claim 4,
Wherein the additive comprises an active stabilizer, wherein the active stabilizer comprises at least one of albumin, elastin, PRP, Human serum, Cell Culture media, Cell Conditioned Media, Bovine surum, and hyaluronic acid.

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