KR20160093349A - Mineral-peptides comprising minerals from ligneous clay, their preparation method and their use - Google Patents

Abstract

The present invention relates to mineral-peptides using minerals from ligneous clay and a production method and usage thereof. The present invention provides the mineral-peptides obtained by reacting a mineral extract with oligopeptide having amino acid residues 2-10 or with protein, wherein the mineral extract is obtained by using an acidic aqueous solution or an aqueous alkaline solution to obtain minerals from ligneous. The mineral-peptides of the present invention constitutes a stable condition and is easy to use and is easily absorbed, thereby replacing conventional sources of minerals or protein such as amino acid or a peptide to be used as an effective supply source of natural minerals and protein in various areas such as cosmetic products, animal feeds, food additives and foods.

Description

Mineral peptides using minerals of woody clay, their preparation methods and their uses, and their use < RTI ID = 0.0 >

The present invention relates to a method of using minerals of woody clay and a use thereof, and more particularly to the production of mineral peptides by using minerals of woody clay and its application to various uses.

Kaolin is mainly composed of kaolinite and halloysite, and feldspar is formed by chemical weathering with carbonic acid and water. The layered kaolin resulting from the transportation and deposition of such kaolin is called woody clay. In the present invention, "ligneous clay" is meant to include all kaolin including layered kaolin unless specifically limited.

Woody clay is used not only as a raw material for ceramics but also as a fertilizer or a livestock feed for supplementing nutrients such as minerals in the soil due to high organic and mineral content (Korean Patent Laid-Open Publication No. 10-2007-0046526, 2004-0088785, Laid-open Patent Publication No. 10-2010-0006051). When woody clay is used in its natural state, woody clay has a high mineral content and generally contains a large amount of organic matter, but it takes a long time to be decomposed and used by microorganisms in the soil. Moreover, even if minerals are absorbed and utilized, only a very small portion of the active ingredients of the woody clay is utilized, and since a large amount of woody clay is applied to the ground and only a small amount is used, the woody clay is used as fertilizer It is very uneconomical.

Accordingly, methods for separating and using minerals in woody clay have been proposed. Korean Patent Publication Nos. 10-2013-0119754, 10-2005-0038317 and 10-2011-0003905 disclose a method for using minerals separated from kaolin in cosmetics and health foods. However, when the minerals are simply separated from kaolin, the stability of the mineral is low and it is easily lost when applied to the soil, so the effect is not continuous, the utilization rate to the crop is low compared with the input amount, Is not high.

Korean Patent Publication No. 10-2007-0046526 Korean Patent Publication No. 10-2004-0088785 Korean Patent Publication No. 10-2010-0006051 Korean Patent Publication No. 10-2013-0119754 Korean Patent Publication No. 10-2005-0038317 Korean Patent Publication No. 10-2011-0003905

If you can effectively extract minerals from woody clay, you can use them as a source of natural minerals in various fields. Furthermore, since the extracted minerals are combined with the oligopeptides to produce a mineral peptide, it is possible to maintain a stable state. Therefore, it is possible to supply effective minerals in a small amount and to supply minerals and peptides with high availability which can be absorbed and utilized. .

It is an object of the present invention to provide a method for producing a mineral peptide by extracting minerals contained in woody clay, and to provide the mineral peptide thus prepared and its use. In particular, it is an object of the present invention to provide a mineral peptide obtained by extracting and separating minerals from woody clay and binding it to an oligopeptide, and a method for producing the same, and also to a method for producing a mineral peptide using the mineral peptide, And to provide a mineral peptide product of the present invention.

In the present invention,

There is provided a mineral peptide obtained by reacting 2 to 10 oligopeptides or proteins of amino acid residues with a mineral extract obtained by extracting minerals of woody clay with an acidic aqueous solution or an alkaline aqueous solution.

A method for producing a mineral peptide of the present invention using minerals of woody clay,

(a) dissolving the woody clay in an acidic aqueous solution or an aqueous alkali solution having a weight of 3 to 30 times by weight at 40 to 60 ° C for 2 to 20 hours, and then separating and removing the solid content to obtain a mineral extract;

(b) adjusting the pH of the mineral extract obtained in the step (a) to 8.5 to 11, adding 2 to 10 amino acid residues of the oligopeptide at a temperature of 60 to 100 ° C, and reacting the mineral extract for 1 to 5 hours to obtain a mineral peptide .

Another method for producing a mineral peptide of the present invention comprises:

(a) dissolving the woody clay in an acidic aqueous solution or an aqueous alkali solution having a weight of 3 to 30 times by weight at 40 to 60 ° C for 2 to 20 hours, and then separating and removing the solid content to obtain a mineral extract;

(b) the mineral extract obtained in step (a) is selected from collagen, milk peptide, keratin, gelatin, vegetable protein, animal protein and marine protein while adjusting the pH of the mineral extract solution to 8.5 to 11 and maintaining the temperature at 50 to 60 ° C. Adding one or more proteins and a protein hydrolyzing enzyme and reacting to obtain a mineral peptide.

In the present invention, mineral peptide products such as fertilizers, cosmetics, feeds, food additives and foods containing the mineral peptides are provided as active ingredients for supplying minerals and proteins (peptides, amino acids).

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to obtain a wood-based clay-derived mineral peptide in which an effective amount of an inorganic substance is effectively extracted from wood clay and bound to a low molecular weight peptide. Since the mineral peptide of the present invention has a stable state and is easy to use and absorb, it can be used as an effective source of natural minerals and proteins in a variety of fields such as fertilizer, cosmetics, feed, food additive, food, ) Circle.

Fig. 1 shows the result of analyzing the mass of the oligopeptide obtained in Example 1. Fig.
Fig. 2 shows the result of mass analysis of the mineral peptide obtained in Example 1. Fig.

Although the term used in the present invention is a term that is generally used in the technical field as much as possible, in some cases, there is a term in which the applicant arbitrarily defines the meaning thereof. In this case, The meaning should be understood in consideration of the meaning written or used throughout the specification.

In the present invention, the term "mineral peptide" means a complex of minerals and oligopeptides derived from woody clay.

The mineral peptide of the present invention is a complex of minerals and oligopeptides extracted from woody clay, and is a complex in which a mineral extracted from an acidic or alkaline aqueous solution is formed by bonding a peptide having about 2 to 10 amino acid residues with a chelate or the like.

Typical components of woody clay are silicate, aluminum trioxide, calcium oxide, iron oxide, magnesium oxide and other oxides. Woody clay is a soil mineral composed of these oxides. The woody clay has a two-story or three-story structure, and contains Zeolite, Bentonite, Kaolin, biotite and sericite, and is mainly composed of kaolinite group minerals. The chemical formula is Al2Si2O5 (OH), and a representative kaolinite crystal structure is composed of one Si4O10 tetrahedral layer and one Al4O4 (OH) 4 octahedral layer. According to the data analyzed by the Korea Testing and Research Institute, more than 57.2% is composed of silicate compounds and the aluminum nitrate compound is more than 14.3%. Table 1 below shows the compositional analysis of woody clay powder analyzed by the Korea Testing and Research Institute.

[Table 1]

Table 2 shows the analysis results of the harmful components of the samples (woody clay dry powder) disclosed as fertilizer.

[Table 2]

Ingredients Standards: Rural Development Administration Notification No. 2012-22 List of Eco-Friendly Organic Material List Disclosure Standards and Quality Standards (ppm)

Although woody clay contains a lot of minerals, very small amounts of minerals are detected when extracting at the highest temperature of 40 ℃ where the crops grow using neutral agricultural water, usually near pH 5.5 ~ 8.5 . However, according to the present invention, in case of extracting minerals derived from an organism, the obtained minerals exhibit a high extraction efficiency such that the total concentration reaches 20%. Important minerals (insoluble), which are generally not extracted by agricultural water, can also be used in the extraction of organic acids and peptides of the present invention. According to the woody clay extraction method of the present invention described below, the woody clay is not completely dissolved completely. Components that are insoluble in the woody clay or present in a form that the inorganic form can not dissolve in the acid and alkaline regions remain as residues. The woody clay residue remaining after the extraction can be used separately as a cosmetic pack or a cover for farmland, and can also be used as a material for making onggi and porcelain, which is the original use of kaolin.

In the present invention, the oligopeptide bound to the minerals derived from woody clay to form a mineral peptide has about 2 to 10 amino acid residues, and more preferably about 2 to about 6 amino acid residues. Alternatively, the oligopeptide may be added to the aqueous solution of minerals derived from woody clay. Alternatively, the protein and the protein hydrolyzing enzyme may be added in place of the oligopeptide to bind to the mineral while decomposing into an oligopeptide.

The method for preparing a mineral peptide using the minerals of woody clay according to the present invention comprises the steps of obtaining a mineral extract from largely clay, and adding a peptide or protein to the mineral extract and reacting to obtain a mineral peptide. Hereinafter, each step will be described in detail.

Step of obtaining mineral extract from woody clay

In this step, minerals are extracted by dissolving the woody clay in an acidic aqueous solution or an aqueous alkali solution for a certain period of time, and then the solid content is separated and removed to obtain a mineral extract.

Preferably, the woody clay is dissolved in an acidic aqueous solution of 3 to 30 times by weight or an aqueous alkali solution, more preferably 5 to 20 times by weight of an acidic aqueous solution or an aqueous alkaline solution. At this time, the temperature of the acidic aqueous solution or the aqueous alkaline solution is preferably 40 to 60 ° C. The time for dissolving in the above temperature range is preferably about 2 to 20 hours, more preferably 4 hours or more.

The separation of solid components after dissolution can be performed by centrifugation or sedimentation separation, but is not limited thereto.

The acid in the acidic aqueous solution is preferably an organic acid. The acid is more preferably at least one selected from the group consisting of citric acid, acetic acid and hydrochloric acid. Particularly suitable acids may be chosen according to the final desired mineral peptide product, with citric acid being a particularly suitable example for cosmetics, and acetic acid being particularly suitable for foods. After extraction in an acidic aqueous solution, it may be neutralized with alkali to use minerals derived from woody clay alone as minerals.

In the alkali aqueous solution, the alkali is preferably at least one of KOH, NaOH, and Ca (OH) ₂ alkaline amino acids. After extraction in an aqueous alkaline solution, it is neutralized with an organic acid, so that minerals derived from woody clay can be used singly for minerals.

In the process of extracting or neutralizing with an acidic aqueous solution or an alkaline aqueous solution as described above, the content of minerals such as K, Na, Ca may be adjusted to a desired level as necessary. That is, the content in the mineral extract obtained by appropriately controlling the amount of KOH, NaOH, Ca (OH) _2, etc. used in the extraction or neutralization can be controlled.

Steps to Obtain Mineral Peptides

The step of obtaining the mineral peptide from the mineral extract thus obtained can be largely performed in two ways.

The first method is to add the oligopeptide to the mineral extract solution obtained above and to react. At this time, the pH of the mineral extract is adjusted to 8.5 to 11, and 2 to 10 amino acid residues of the oligopeptide are added while maintaining the temperature of the solution at 60 to 100 ° C, and the reaction is carried out for 1 to 5 hours to obtain mineral peptides. More preferably, the temperature of the mineral extract solution is adjusted to 70 ° C or higher, and the oligopeptide is mixed and allowed to react with stirring for 2 hours or more. Preferably, the pH of the mineral extract is adjusted to 8.5 to 11 by adding at least one of KOH, NaOH, Ca (OH) ₂ , arginine and lysine.

In the second method, the protein and the protein hydrolyzing enzyme are added to the mineral extract solution obtained above and reacted. First, the pH of the mineral extract is adjusted to 8.5 to 11, and the temperature of the solution is maintained at 50 to 60 ° C., and at least one protein selected from collagen, milk peptide, keratin, gelatin, vegetable protein, animal protein, Hydrolysis enzymes are added and reacted. The preferred reaction time in the temperature range is 2 to 24 hours. The reaction time is related to the molecular weight of the peptide. When the peptide having an average molecular weight of about 1000 (mole weight) is used, the reaction can be preferably carried out for 8 to 12 hours.

The protein hydrolyzing enzyme can be used without limitation as long as it is capable of hydrolyzing the protein, and preferably includes any one of papain, trypsin alkalase-derived protease, and protease.

This method uses a filter press, centrifugation, sedimentation, etc. for separation after the reaction, but the concentration of the solution is lowered to obtain a smooth effect. Therefore, the amount obtained is somewhat higher than the first method, but the concentration is lowered.

Since the quality of the desired mineral peptide may vary depending on the desired mineral peptide product, one of ordinary skill in the art would be able to choose a suitable method according to the purpose of the above methods.

The mineral peptide obtained according to the present invention can be used as an active ingredient for supplying minerals and proteins in various fields. The mineral peptide of the present invention can be included as an active ingredient for supplying minerals and proteins to fertilizers, cosmetics, feeds, food additives, foods (including health foods) in particular.

[Example]

Hereinafter, the present invention will be described in more detail with reference to specific examples. However, these embodiments are only for describing the present invention more specifically, and the scope of the present invention is not limited by these embodiments.

≪ Example 1 >

1. Preparation of mineral extract

200 g of the dried woody clay was dispersed in 1.8 kg of a 0.3% citric acid solution, and the mixture was stirred at a temperature of 60 캜 for 6 hours to extract minerals. Then, the sample was neutralized with NaOH, diluted 10 times, and the content of minerals was measured by ICP. An inductively coupled plasma mass spectrometer was used for the analysis, and the results are shown in Table 3 below.

[Table 3]

* The conversion ratio is 10% (10 times dilution) and the dilution factor is 10 times, so it corresponds to 100 times dilution as a whole and converted to the measurement concentration 100 times.

**% The total mineral concentration corresponds to 11.85%. That is, the concentration of minerals that can be dissolved and extracted from the total dry weight of the woody clay.

2. Preparation of Mineral Peptides

(1) oligopeptide

The enzyme was hydrolyzed at 55 ° C for 24 hours to adjust the pH to 10 with KOH 20% solution using an alkaline (proteolytic enzyme) as an enzyme in whey gelatin (Sammi Industries) to obtain oligopeptides. The mass of the obtained oligopeptide was analyzed by the National Institute of Chemical Technology of Japan. The results are shown in Fig.

(2) Mineral peptides

The pH of the mineral extract obtained in 1 was adjusted to about 10 by adding an aqueous NaOH solution. Then, the oligopeptide prepared above was added while maintaining the temperature of the solution at about 80 ° C, and the reaction was carried out with stirring for about 3 hours to obtain a mineral peptide. The mass of the obtained mineral peptide was analyzed by the National Institute of Chemical Technology of Japan and the results are shown in FIG.

≪ Example 2 >

Ineffectiveness of Mineral Peptides on the Growth of Common Crops

Lettuce, and cucumber as experimental crops, the ineffectiveness and the ineffectiveness of the mineral peptides obtained in Example 1 on the growth of the crops were tested.

One. How to grow

1) Disclosed crops - lettuce (growth of leaf and crop growth), cucumber (comparative measurement)

2) Measurement contents - Leaf number, leaf length, leaf width, chlorophyll content

3) Growth process and observation

   Growth process - 3 - 4 leaves of lettuce and cucumber were planted.

   - Sowing on October 10 and observing in greenhouse on October 25.

4) Cultivated yields were selected and cultivated by selecting 10 minerals according to supply condition of minerals.

5) Feeding the same amount of water according to the feeding period,

- dry woody clay diluted in general agricultural water (groundwater) 5g / L

- Mineral peptide obtained in Example 1 0.1 g / L: 5 g / L Mineral extract + Peptide

(The concentration of the mineral extract of 5 g / L is 0.5925 g / L - 592.5 ppm in terms of the content of the total minerals contained)

6) Classification of mineral supply conditions

- No processing: a

- Dilute dry woody clay to general agricultural water (groundwater): b

- Mineral peptide supply of Example 1: c

≪ RTI ID = 0.0 > oligopeptide < / RTI > obtained in Example 1: d

2. Cultivation Result

1) Effect on leaf number - Lettuce

 2) Effect on leaf area - lettuce

3) Effect on leaf width - Lettuce

 4) Effect on chlorophyll content - lettuce

 * Chlorophyllometer: SPAD-502 chlorophyll meter

 5) Compared to the test result - Cucumber

* Compared to 0 ~ 5

3. Evaluation of results

1) Experimental group (c) to which the mineral peptide of the present invention was fed in the growth promoting effect of the present invention was an experimental group (b) in which a minerals extract solution was fed as well as an experimental group (d).

2) Vegetables grew in a soft state at the beginning of the growth process, and after a certain period of time, the luster and thickness of the leaves became better as the harvester entered the harvesting season, and the appearance was fresh. Sensory observations also showed that the groups that supplied the woody clay extract had superior leaf thickness and luster. The chlorophyll content analyzed above also supports this sensory result.

Claims (17)

(a) dissolving the woody clay in an acidic aqueous solution or an aqueous alkali solution having a weight of 3 to 30 times by weight at 40 to 60 ° C for 2 to 20 hours, and then separating and removing the solid content to obtain a mineral extract;
(b) adjusting the pH of the mineral extract obtained in the step (a) to 8.5 to 11, adding 2 to 10 amino acid residues of the oligopeptide at a temperature of 60 to 100 ° C, and reacting the mineral extract for 1 to 5 hours to obtain a mineral peptide step
≪ / RTI > wherein the method comprises the steps of: The method according to claim 1,
Wherein the mineral clay is dissolved in an acidic aqueous solution having a weight of 3 to 30 times by weight at 40 to 60 ° C for 2 to 20 hours to obtain a mineral extract solution. In step (b), the pH of the mineral extract solution is adjusted to 8.5 - 11. The method for producing a mineral peptide using the mineral of woody clay according to claim 1, 3. The method of claim 2,
Wherein the acidic aqueous solution is an aqueous solution of at least one organic acid selected from the group consisting of citric acid, acetic acid and hydrochloric acid. 3. The method of claim 2,
Wherein the pH of the mineral extract is adjusted to 8.5 to 11 by adding at least one of KOH, NaOH, Ca (OH) ₂ , arginine and lysine in the step (b) Gt; The method according to claim 1,
Wherein the mineral clay is dissolved in an alkali aqueous solution having a weight of 3 to 30 times by weight at 40 to 60 ° C for 2 to 20 hours to obtain a mineral extract solution in the step (a). 6. The method of claim 5,
Wherein the alkali aqueous solution is an aqueous solution of at least one of KOH, NaOH, and Ca (OH) ₂ alkaline amino acids. 7. The method according to any one of claims 1 to 6,
Further comprising, following the step (b), neutralizing the obtained mineral peptide with an organic acid or an alkali, thereby producing a mineral peptide using the minerals of woody clay. (a) dissolving the woody clay in an acidic aqueous solution or an aqueous alkali solution having a weight of 3 to 30 times by weight at 40 to 60 ° C for 2 to 20 hours, and then separating and removing the solid content to obtain a mineral extract;
(b) the mineral extract obtained in step (a) is selected from collagen, milk peptide, keratin, gelatin, vegetable protein, animal protein and marine protein while adjusting the pH of the mineral extract solution to 8.5 to 11 and maintaining the temperature at 50 to 60 ° C. Adding one or more proteins and a protein hydrolyzing enzyme and reacting to obtain a mineral peptide
≪ / RTI > wherein the method comprises the steps of: 9. The method of claim 8,
Wherein the protein hydrolyzing enzyme comprises any one of papain, protease derived from trypsin alkalase, and protease, and a method for producing a mineral peptide using the minerals of woody clay. A mineral peptide obtained by reacting 2 to 10 amino acid residues of an oligopeptide or protein with a mineral extract obtained by extracting the minerals of woody clay with an aqueous acid solution or alkali solution. 11. The method of claim 10,
Wherein the acidic aqueous solution is an aqueous solution of at least one organic acid selected from the group consisting of citric acid, acetic acid and hydrochloric acid. 11. The method of claim 10,
Wherein the alkali aqueous solution is an aqueous solution of at least one of KOH, NaOH, and Ca (OH) ₂ alkaline amino acids. 11. The method of claim 10,
Wherein the protein hydrolase comprises any one of papain, trypsin alkalase-derived protease, and protease. 14. A fertilizer composition comprising the mineral peptide of any one of claims 10 to 13 as an active ingredient for the supply of minerals and proteins. 14. A feed composition comprising the mineral peptide of any one of claims 10 to 13 as an active ingredient for minerals and protein feed. 14. A cosmetic composition comprising the mineral peptide of any one of claims 10 to 13 as an active ingredient for the supply of minerals and proteins. 14. A food composition comprising the mineral peptide of any one of claims 10 to 13 as an active ingredient for the supply of minerals and proteins.

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