KR20160081178A - Skin external composition for moisturing skin or whitening skin comprising catechins and icariside b2 - Google Patents

Abstract

The present invention relates to a composition for external applications to the skin, comprising catechin and icariside B2 as active ingredients. The composition for external applications to the skin of the present invention exhibits excellent effects in whitening the skin and effects in moisturizing the skin in comparison with the case of each of the catechin and the icariside B2 used in a separate manner.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for external use for skin moisturizing or whitening comprising a catechin and an icaricide B2,

The present invention relates to a composition for external application for skin containing catechin and icaricide B2 and having excellent moisturizing and whitening effect.

Skin plays a very important role as a barrier function to protect individuals from the outside. The barrier function is a protection function for protecting against various stimuli (chemicals, air pollutants, dry environment, ultraviolet rays, etc.) from the outside and excessive divergence of water in the body through the skin. Such protection function is a function of protecting the stratum corneum The function can be maintained only when it is normally formed.

The outermost stratum corneum (horney layer) of the epidermis is formed from keratinocytes and consists of keratinocytes with complete differentiation and a surrounding lipid layer (Marcelo CL et al . , J. Invest . Dermatol . , 80 , pp 37-44, 1983).

The keratinocyte is a characteristic cell in which basal cells continuously proliferating in the lowest epidermis undergo a stepwise change in morphology and function and are elevated to the surface of the skin. After a certain period of time, And the new keratinocyte replaces its function. This repetitive sequence of changes is called "epidermal cell differentiation" or "keratinization".

In addition, during keratinization, keratinocytes form natural moisturizing factors (NMF) and intercellular lipids (ceramides, cholesterol, fatty acids) and form stratum corneum, which gives firmness and flexibility to the stratum corneum, barrier function.

Such stratum corneum can easily lose its function due to environmental factors such as excessive washing, bathing and other lifestyle factors, dry air pollutants, and endogenous diseases such as atopic skin and senile skin. In fact, Due to the increasing number of factors, dry skin symptoms and their complaints have been increasing recently.

Therefore, in order to maintain proper skin moisture, various studies have been made to supply water from the outside or to prevent water loss from the body. Various moisturizers having moisture retention ability have been developed. As a skin moisturizing agent, it is common to use a substance capable of increasing water retention in the stratum corneum such as ceramide and essential fatty acid and lipid complex (Rawlings AV et al . , J. Invest . Dermatol . , 5, pp731- 741, 1994).

However, recently, the risk factors for skin are increasing, and changes in dietary patterns slow down the generation and decline of the stratum corneum, and the function of the keratinocyte is reduced, so that the amount of the moisturizing factor and lipid in the stratum corneum is decreased, There is an increasing trend in people with skin that can not function as a skin barrier. Such breakdown of the skin barrier function causes various skin diseases such as dry skin, atopic dermatitis, contact dermatitis and psoriasis. Such diseases can be expected to be alleviated by moisturizing agents having a conventional moisture retention function but it is difficult to achieve fundamental healing.

In addition, human skin color is determined by various factors such as the activity of melanocyte making melanocyte, the distribution of blood vessels, the thickness of skin, and the presence or absence of pigment inside and outside the body, such as carotenoids and bilirubin. In particular, melanin, which is produced by the action of various enzymes such as tyrosinase in melanocytes, is the most important factor. The formation of melanin pigment is affected by environmental factors such as genetic factors, hormonal secretion, physiological factors such as stress, and ultraviolet irradiation. Melanin is present in the skin and protects the body from ultraviolet rays, but it is known that melanin is overproduced, thereby promoting pigmentation and aging of the skin and leading to skin cancer induction. (Ascorbic acid, kojic adcid, arbutin, hudroquinone) have been previously used to treat or relieve excessive melanin pigmentation caused by skin pigmentation abnormalities and ultraviolet exposure. Glutathione or a derivative thereof and a substance having inhibitory activity against tyrosinase have been used in cosmetics or medicines in combination with insufficient whitening effect, safety to the skin, Its use is restricted due to stability problems and so on.

On the other hand, the icari seed B2 is a perennial plant belonging to the fern-tail gosarit family ( Asplenium Interface Variable Name Carcinoid derived from a portion of scolopendrium) (isolated from terpenoid) component, or chemically synthesized can also (Natural Product Sciences, 14 (4 ): 265-268 (2008)). As to the icaricide compound icaride II (icariside II), whitening activity is already known (International Patent Publication No. WO 2008/035918, published on Mar. 27, 2008). On the other hand, the studies on icaricide B2, which is an icaricide compound, are insignificant, and there has not yet been reported remarkable research results on the efficacy shown in relation to the improvement of the skin condition and the synergistic effect through combination with other ingredients.

Korean Patent Laid-Open No. 10-2012-0083943 (published on July 27, 2012) International Patent Publication No. WO 2008/035918 (published on Mar. 27, 2008)

Natural Product Sciences, 14 (4): 265-268 (2008)

The present invention provides a composition for external application for skin moisturizing which contains catechin and icaricide B2 as an active ingredient and has excellent skin moisturizing effect and skin whitening effect.

To achieve the above object, the present invention provides a composition for external application for skin for moisturizing or whitening comprising catechin and icaricide B2 as an active ingredient.

The composition for external application for skin according to the present invention shows excellent skin moisturizing effect and skin whitening effect by containing low-concentration catechin and icaricide B2 as an active ingredient.

Hereinafter, the present invention will be described in more detail.

The catechins used in the present invention were extracted from green tea and mainly composed of EGCG, GCG, ECG and CG, but not limited to the above catechins.

Green tea ( Camellia sinensis ) is an evergreen tree of Camellia sinensis . It is generally called green tea because its shoots or leaves have not been fermented during the manufacturing process. It has been reported that green tea was drunk from the new agriculture of China in 2700 BC, and it is said that Korea drank tea from the time of Silla Dynasty. In addition, green tea contains catechin, flavonoid, theanine, GABA, caffeine, tannin, and various vitamins, which are used for anticancer, antioxidant, antibacterial, deodorant, blood pressure Depression, and immune function are known to have a variety of effects, such as recently reported to be effective in dieting. Particularly, catechin is a major functional ingredient of green tea which exhibits various pharmacological actions ranging from antioxidant, anticancer, antibacterial, and prevention of heart disease.

The catechins contained in green tea were catechin (CA), epicatechin (EC), epigallocatechin (EGC), EGCG, and epigallocatechin (EGC) have.

In the present invention, the catechin is selected from the group consisting of EGCG (epigallocatechin gallate), GCG (gallocatechin gallate), ECG (epicatechin gallate), CG (catechin gallate), EGC (epigallocatechin) ), (-) EC (epicatechin), (+) EC (epicatechin), (-) CA (catechin) and (+) CA. Green tea extract is preferably extracted from green tea leaves.

The composition according to the present invention may contain catechins in an amount of 0.001 to 20% by weight, preferably 0.1 to 10% by weight, based on the total weight of the composition. If the content of the catechin is less than 0.001% by weight, the effect and effect of the component are insignificant. If the content exceeds 20% by weight, there is a problem of skin safety or shape.

The icaricide B2 used in the present invention has a structure represented by the following formula (1).

[Chemical Formula 1]

The icaricide B2 of the present invention may be extracted from plants, synthesized according to methods known in the art, or commercially available ones may be used.

The composition according to the present invention may contain the icarissid B2 in an amount of 0.001 to 20% by weight, preferably 0.1 to 10% by weight, based on the total weight of the composition. If the content of the icaricide B2 is less than 0.001% by weight, the effect and effect of the ingredient are insignificant. If the content exceeds 20% by weight, there is a problem of skin safety or shape.

The composition for external application for skin according to the present invention can effectively improve symptoms such as wrinkles and elasticity by using catechin and icaricide B2 together.

The composition for external application for skin according to the present invention may be used for enhancing the skin barrier function,

And can be usefully used as a composition for external application for skin to prevent or ameliorate skin dryness, atopic dermatitis, contact dermatitis, psoriasis or the like caused by incomplete epidermal differentiation.

In addition, the composition for external application for skin according to the present invention has excellent ability to recover damaged skin barrier and increase skin moisture content, and can be used as a composition for skin regeneration.

In addition, the composition for external application for skin according to the present invention has excellent ability to recover damaged skin barrier and increase skin moisture content, and can be used as a composition for skin regeneration.

The above composition for external application for skin of the present invention can be formulated as a cosmetic composition, and can be formulated containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example as a solution, a gel, a solid, a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic form (liposome) In the form of ionic fibrin dispersions, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. It can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions may be prepared according to conventional methods in the art.

The cosmetic composition according to the present invention can be applied to cosmetics such as softening agents, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, And body essence.

In addition, the composition according to the present invention may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening agents, gelling agents, softening agents, antioxidants, suspending agents, stabilizing agents, Such as fillers, emulsifiers, emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, moisturizers, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics May contain adjuvants commonly used in the field of cosmetics or dermatology. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the skin improving effect.

Hereinafter, the present invention will be described in more detail with reference to examples and test examples, but the present invention is not limited to these examples.

[Referential Example 1]

Epigallocatechin (EGCG) was purchased from Sigma-Aldrich to test the efficacy of the composition of the present invention.

[Reference Example 2]

Icaricid B2 for testing the efficacy of the composition of the present invention was purchased from Simagchem Corporation (China).

[Test Example 1] Measurement of promoting effect of keratinocyte differentiation

In order to examine the differentiation promoting effect of the composition for external application for skin according to the present invention, the amount of corned envelope (CE) generated upon differentiation of keratinocytes was measured by absorbance as described below.

First, keratinocytes (HEK; Lonza Co., NHEK-Neonatal Normal Human Epidermal Keratinocytes, Pooled) isolated from epidermis of a newborn baby were placed in a culture flask and adhered to the bottom. (2 mg of catechin (2 mg of catechin diluted with 1 L of distilled water, concentration of 2.5 ppm; 2.5 mg of catechin diluted with 1 L of distilled water, concentration of 3 ppm; 3 mg of catechin was dissolved in 1 L of distilled water Diluted with 1 L of distilled water to a concentration of 2.5 ppm, 2.5 mg of icaricide B2 was diluted with 1 L of distilled water to a concentration of 3 ppm, 3 mg of icaricide B2 (concentration of 2 ppm of the icaricide B2 in Reference Example 2) (0.03 mM) and high calcium (1.2 mM) were treated with a negative control and a positive control, respectively, for 5 days until the cells were grown to 70 to 80% of the floor area The catechin of Reference Example 1 (concentration of 5 ppm; 5 mg of catechin was diluted with 1 L of distilled water) and icaricide B2 (5 ppm concentration of Reference Example 2, 5 mg of icaricide B2 was diluted with 1 L of distilled water). Then, the cultured cells were harvested and washed with PBS buffered saline) and 1 ml of a 10 mM Tris-HCl buffer (Tris-HCl, pH 7.4) containing 2% SDS (sodium dodecyl sulfate) and 20 mM DTT (Dithiothreitol) Boiling and centrifugation, and the precipitate was again suspended in 1 ml of PBS to measure the absorbance at 340 nm. Separately, a part of the solution after the sonication was taken to measure the protein content, and based on the evaluation of the degree of cell differentiation The results are shown in Table 1 below.

Test substance The ability to differentiate in keratinocytes (%) Low calcium (0.03 mM) solution
(Negative control) 100 High calcium (1.2 mM) solution
(Positive control group) 210 Catechin (2.5 ppm) +
Icaricid B2 (2.5 ppm) 306 Catechin (3 ppm) +
Icaricid B2 (2 ppm) 313 Catechin (2 ppm) +
Icaricid B2 (3 ppm) 323 Catechin (5 ppm) 289 Icaricid B2 (5 ppm) 224

As shown in Table 1, it was confirmed that when catechin or icaricide B2 alone was treated, the differentiation promoting effect of keratin-forming cells was present, and when catechin and icaricide B2 were treated together, It was confirmed that the effect of promoting differentiation of cells was excellent.

In addition, the treatment with catechin (3 ppm) + icaricide B2 (2 ppm) was superior to the treatment with catechin (2.5 ppm) + icaricide B2 (2.5 ppm) , Catechin (2 ppm) + icaricide B2 (3 ppm) was more excellent in promoting the differentiation of keratinocytes.

[Test Example 2] Measurement of skin barrier function recovery effect

In order to measure the effect of the composition for external application for skin according to the present invention on the recovery of damaged skin barrier function due to skin damage, the following experiment was conducted. Ten adult male and female adult skin was damaged by damaging the skin barrier using a tape stripping method, and while applying the four groups of Formulation Example 1 and Comparative Formulation Examples 1 to 3 prepared in the composition shown in Table 2 below, 7 (TWEL) was measured and compared with a Vapometer (Delfin, Finland) once a day for one day. Comparative Formulation Example 1 is a vehicle as a negative control, Comparative Formulation Example 2 is a comparative control containing the catechin of Reference Example 1, Comparative Formulation 3 is a comparative control containing the icaricide B2 of Reference Example 2 to be. The experimental results are shown in Table 3 below. The results in Table 3 were compared before treatment before barrier damage and after treatment before barrier damage on the basis of 100%.

Compounding ingredient Formulation Example 1 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 Purified water 69 70 69 69 Propylene glycol 30 30 30 30 Catechin 0.5 - One - Icari seed B2 0.5 - - One

Test group TWEL Change (%) Before processing 1 day 2 days 3 days 4 days 5 days 6 days Formulation Example 1 100 139 135 132 128 121 120 Comparative Formulation Example 1 100 121 112 98 70 62 43 Comparative Formulation Example 2 100 129 127 123 123 114 112 Comparative Formulation Example 3 100 124 119 112 111 109 106

As shown in Table 3, when catechin and icaricide B2 were treated together, the amount of transdermal water loss returned to normal at a faster rate than when catechin or icaricide B2 alone was treated by synergy, It can be confirmed that it is recovered.

[Reference Example 3] Formulation Examples 2 and 3 and Comparative Formulation Examples 4 to 6

Nutritive creams were prepared according to the composition of Table 4 below in a conventional manner (unit: wt%).

Compounding ingredient Formulation Example 2 Formulation Example 3 Comparative Formulation Example 4 Comparative Formulation Example 5 Comparative Formulation Example 6 Purified water To 100 To 100 To 100 To 100 To 100 EGCG 0.25 0.2 - 0.5 - Icari seed B2 0.25 0.3 - - 0.5 Vegetable hydrogenated oil 1.50 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 0.60 Glycerol stearate 1.00 1.00 1.00 1.00 1.00 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 Polyglyceryl-10pentastearate and behenyl alcohol and sodium stearoyl lactylate 1.00 1.00 1.00 1.00 1.00 Arachidyl behenyl alcohol and arachidyl glucoside 1.00 1.00 1.00 1.00 1.00 Cetylaryl Alcohol and Cetearyl Glucoside 2.00 2.00 2.00 2.00 2.00 PEG-100 Stearate & Glycerololate & Propylene Glycol 1.50 1.50 1.50 1.50 1.50 Caprylic / carboxy triglyceride 11.00 11.00 11.00 11.00 11.00 Cyclomedicone 6.00 6.00 6.00 6.00 6.00 Preservative, incense Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Triethanolamine 0.1 0.1 0.1 0.1 0.1

 [Test Example 4] Measurement of skin moisturizing effect

In order to measure the effect of the composition of the present invention on the skin moisturizing effect, Formulation Examples 2 and 3 and Comparative Formulation Example 6 were used and evaluated as follows.

50 men and women in the 40s and 50s classified as dry skin were divided into 5 groups of 10 persons for each of Formulation Examples 2, 3 and Comparative Formulation Examples 4 to 6, and the nutritional cream was applied twice daily for 4 weeks to the face . (24 ° C, relative humidity 40%) after 1 week, 2 weeks, and 4 weeks after application, and after 2 weeks (total 6 weeks) (Corneometer CM825, C + K Electronic Co., Germany). The results are shown in Table 5 below. The results in Table 5 are based on the skin moisture meter measured just before the start of the test, and the increase in the measured value after a certain period of treatment is expressed as a percentage.

Test group Moisture growth rate (%) 1 week Two weeks 4 weeks 6 weeks Formulation Example 2 46 44 42 40 Formulation Example 3 48 46 44 43 Comparative Formulation Example 4 30 32 32 15 Comparative Formulation Example 5 35 38 37 34 Comparative Formulation Example 6 32 35 34 32

The results of Table 5 show that when Comparative Formulation Example 4 was applied, the moisture increase rate was about 30% until 4 weeks of application, but after the application was stopped, the amount of skin water decreased sharply and EGCG alone , Comparative Formulation Example 6, which contained Comparative Formulation Example 5 or icaricide B2 alone, was coated with Formulation Example 2, which contained the EGCG and icaricide B2 together, although the skin moisture increase rate remained mostly at about 30% In one case, it can be confirmed that the syneresis effect shows a skin moisture increase rate of 40% or more even after stopping application. Thus, it can be seen that the composition of the present invention has excellent skin moisturizing effect.

 [Test Example 5] Inhibitory effect on melanin formation by B16 / F10 melanoma cells

The B16 / F10 melanoma used in this test example is a mouse-derived cell line, and is a cancer cell caused by malignant cell which secretes a melanin pigment called melanin. During the artificial incubation of these cells, the test material was treated to compare the degree of decrease in melanin production. The B16 / F10 melanoma used in this test was purchased from Korean Cell Line Bank (KCLB No: 8008).

Melanin biosynthesis inhibitory effect of B16 / F10 melanoma was measured as follows. B16 / F10 melanoma cells were seeded in a 6-well plate at 5 × 10 ⁵ cells / well and cultured in DMEM containing 10% FBS (fetal bovine serum) for 24 hours in a 5% CO ₂ incubator (MCO-20AIC, Dulbecco ' s Modified Eagle ' s Medium). The test material was added to fresh DMEM medium without FBS and aged for 72 hours. The test materials used at this time are as shown in Table 6 below. After treatment with trypsin-EDTA, the cells were separated by centrifugation, and melanin was dissolved at 95 ° C in 1N NaOH containing DMSO. Absorbance was measured at 405 nm using an ELISA reader (DIbiotech, Korea). Cells to which the test substance was not added were used as a control group, and the melanin formation inhibition degree of each test substance was measured by comparing with the melanin content in the control group. The inhibition rate of melanin production was calculated according to Equation (1) and the results are shown in Table 6.

[Equation 1]

Test substance Melanin inhibition rate (%) Control group (no addition) 0 Kojic acid 53 EGCG (0.04% by weight) + icariside B2 (0.06% by weight) 65 EGCG (0.06% by weight) + icariside B2 (0.04% by weight) 61 EGCG (0.05% by weight) + icariside B2 (0.05% by weight) 58 EGCG (0.1% by weight) 51 Icaricid B2 (0.1% by weight) 39

In the results of Table 6 above, it can be confirmed that when EGCG and icaricide B2 are used together, the inhibition of melanin production is increased, and in particular, when they are used in combination according to the present invention, the melanin production inhibitory rate is remarkably improved by the synergistic effect.

 [Test Example 6] Skin tone improvement effect

In order to examine the effect of the composition of the present invention on improving the skin tone, 50 male and female adults in the 20s to 50s were divided into 4 groups of 10 individuals for each of the 5 groups of Formulation Examples 2 and 3 and Comparative Formulations 4 to 6, (Morningx, Japan) using Facial Stage DM-3 (Moritex, Japan) after using Formulation Examples 2 and 3 and Comparative Formulation Examples 4 to 6 (once a day / . The improvement rate of the skin tone was determined by determining the brightness and color change value of the skin from the brightness and color measurement values of the skin, and the average value of the results was obtained and is shown in Table 7 below. The larger the value of brightness and color change, the better the skin tone.

Test substance Skin tone improvement rate (%) brightness Color Formulation Example 2 21 19 Formulation Example 3 24 20 Comparative Formulation Example 4 10 9 Comparative Formulation Example 5 17 15 Comparative Formulation Example 6 14 12

The results of Table 7 show that Comparative Formulation Example 4, which does not contain either EGCG or icaricide B2, shows no significant skin tone improving effect, whereas Comparative Formulations 5 and 6, which contain EGCG or icaricide B2, The skin tone is improved. In the case of Formulation Examples 2 and 3 which simultaneously contain these two components, the skin tone is remarkably improved by the synergy effect.

Hereinafter, formulation examples of the composition according to the present invention will be described. However, the pharmaceutical composition and the cosmetic composition can be applied to various formulations, and the present invention is not limited thereto but is specifically described.

[Formulation Example 1] Lotion

Lotions are prepared according to a conventional method with the composition shown in Table 8 below.

Content (% by weight) Catechin 1.0 Icari seed B2 1.0 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 PEG 12 nonyl phenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 2] Nourishing cream

Nutrition cream is prepared according to a conventional method with the composition shown in Table 9 below.

Content (% by weight) Catechin 1.0 Icari seed B2 1.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 PEG 60 hardened castor oil 2.0 Liquid paraffin 10.0 Squalane 5.0 Caprylic / caproic triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 3] Massage cream

A massage cream is prepared according to a conventional method with the composition shown in Table 10 below.

Content (% by weight) Catechin 0.5 Icari seed B2 0.5 Wax 10.0 Polysorbate 60 1.5 PEG 60 hardened castor oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / capric triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 4] Pack

A pack is prepared according to a conventional method with the composition shown in Table 11 below.

Content (% by weight) Catechin 0.5 Icari seed B2 0.5 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 PEG 12 nonyl phenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 5] Gel

A gel is prepared according to a conventional method with the composition shown in Table 12 below.

Content (% by weight) Catechin 0.25 Icari seed B2 0.25 Ethylenediamine sodium acetate 0.05 glycerin 5.0 Carboxyvinyl polymer 0.3 ethanol 5.0 PEG 60 hardened castor oil 0.5 Triethanolamine 0.3 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 6] Ointment

Ointment was prepared by a conventional method with the composition shown in Table 13 below.

Content (% by weight) Catechin 0.5 Icari seed B2 0.5 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 7] Soft capsule

A soft capsule was prepared by mixing 0.0025 g of catechin, 0.0025 g of icaricide B2, 0.0025 g of vitamin C, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellow radish and 6 mg of lecithin and filling the mixture with 400 mg per capsule according to a conventional method.

[Formulation Example 8] Tablets

0.0025 g of catechin, 0.0025 g of icaricide B2, 0.0025 g of vitamin C, 100 mg of glucose, 96 mg of starch and 4 mg of magnesium stearate were mixed and 40 mg of 30% ethanol was added thereto to form granules. The granules were then dried at 60 ° C, Tablets were tableted.

[Formulation Example 9]

150 mg of catechin, 150 mg of icaricide B2, 150 mg of vitamin C, 100 mg of glucose, and 600 mg of starch were mixed and 100 mg of 30% ethanol was added to form granules, followed by drying at 60 ° C to form granules. The final weight of the contents was 1 g.

[Formulation Example 10]

0.0025 g of catechin, 0.0025 g of icaricide B2, 0.0025 g of vitamin C, 10 g of glucose, 2 g of citric acid and 187.8 g of purified water were mixed and filled in a bottle. The final volume of the contents was adjusted to 200 ml.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (7)

A composition for external application for moisturizing or whitening skin comprising catechin and icaruside B2 represented by the following formula (1) as an active ingredient.
[Chemical Formula 1]

The catechin according to claim 1, wherein the catechin is at least one selected from the group consisting of EGCG (epigallocatechin gallate), GCG (gallocatechin gallate), ECG (epicatechin gallate), and CG Wherein the composition is an external preparation for skin. The composition for external application for skin according to claim 1, wherein the catechin content is 0.001 to 20% by weight based on the total weight of the composition. The composition for external application for skin according to claim 1, wherein the content of the icaricide B2 is 0.001 to 20% by weight based on the total weight of the composition. The composition for external application for skin according to claim 1, wherein the composition is for enhancing skin barrier function. The composition for external application for skin according to claim 1, wherein the composition is for enhancing skin barrier function. The pharmaceutical composition according to claim 1, wherein the composition is for improving color and skin tone.