KR20160067374A - A method for preparing an anti-microbial food packaging film added with the extract of at least one herb consisting of bamboo, hinoki tree and propolis showing preventive effect from food spoilage and the anti-microbial film prepared thereby - Google Patents

Abstract

The present invention relates to a producing method of an anti-microbial film for packaging foods, having excellent food spoilage preventing function, and to an anti-microbial film produced therefrom and, more specifically, to a producing method of an anti-microbial film for packaging foods adding at least one herb medicine extract selected from the group consisting of bamboo, Hinoki cypress and propolis as an anti-microbial additive, which can maintain the freshness of the foods for a long time and can inhibit bacterial decomposition, and to an anti-microbial film produced thereby.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing an antimicrobial film for food packaging, in which at least one herbal medicine extract selected from the group consisting of bamboo, cottonwood, and propolis is added as an antimicrobial additive and an antimicrobial film prepared therefrom The present invention relates to a bamboo, a hinoki tree, and a propolis composition comprising at least one herb consisting of bamboo,

The present invention relates to a process for producing an antimicrobial film for food packaging, in which at least one herbal medicine extract selected from the group consisting of bamboo, whitening wood and propolis having anti-food decay function is added as an antimicrobial additive, and an antibacterial film produced therefrom.

[Patent Document 1] Korean Patent Laid-Open Publication No. 1999-0079669

[Patent Document 2] Korean Patent Laid-Open Publication No. 1999-0031265

[Patent Document 3] Korean Patent Publication No. 2000-0032538

[Literature 4] Information < / RTI > S et al., Illustrated Dictionary of Contemporary Art, Yeonglim Publishing House, p204-208, 1998

[Literature 5] Kuo, Y.-H. et al. (September 2001). "Three novels and one new lignan, chamaecypanonas, B, obtulignolide and isootobanone from the heartwood of Chamaecyparis obtusa var. Tetrahedron Lett. 42 (38): 67316735. doi: 10.1016 / S0040-4039 (01) 01272-2)

[Literature 6] Krauze-Baranowska, M .; Pobłocka, L .; El-Hela, AA (SeptemberOctober 2005). "Biflavones from Chamaecyparis obtusa". Z. Naturforsch. C 60 (910): 679685. PMID 16320608

The present invention relates to a method for producing an antimicrobial film for food packaging, in which at least one herbal medicine extract selected from the group consisting of bamboo, cottonwood and propolis is added as an antimicrobial additive, and to a freshness-maintaining and bacterial decay-inhibiting antimicrobial film produced therefrom, More particularly, the present invention relates to a method for producing an antibacterial film for food packaging, which is capable of maintaining the freshness of food for a long period of time by advancing the degree of corruption very slowly, and suppressing bacterial decay, Freshness maintenance, and antibacterial film for inhibiting bacterial decay.

In general, one of the most important issues in the food processing industry is the development of technologies that can be preserved for a long time by increasing the shelf life of the food. In other words, the indispensable food for human life is more important than any other product, and it is the most important in the field of food related technology to develop the technology that can preserve its quality for a long time.

The preservation period of these foods is determined by various factors such as oxygen, microorganisms, pests or moisture. In order to improve the shelf life of foods, preservatives, which are artificial synthetic additives, have been directly or indirectly added to foods Since these preservatives are harmful substances to the human body, they have a bad influence on the health of the user and adversely affect the taste and flavor inherent to the food. Therefore, a film or a film for food packaging that can replace the preservative is being developed .

In this regard, Korean Patent Laid-Open Publication No. 1999-0079669 discloses an antibacterial polyester film comprising a core layer of a polyester film and a surface layer of an antimicrobial composition. This is because the transparency of the film and the antibacterial property .

These food wrapping films contain many antimicrobial substances such as zeolite, silver nano, silica, or Imazalil, which are synthesized by substituting silver with yellow, oval, biotite, white oak, sapwood, zeolite and sodium Packaging film and antimicrobial agent Benomyl, Nisin, organic acid Propionate, benzoate (polyvinyl alcohol), polyvinyl alcohol (polyvinyl alcohol) (Benzoate), sorbate, wasabi extract, chitosan, and the like.

Korean Patent Laid-Open Publication No. 1999-0031265 discloses a synthetic resin product containing quartzite and a method for producing the same, and is characterized in that one or two of a quartz stone, a biotite, a sericite, a biotite, Although the mixed minerals are mixed with plastic products to produce a somewhat improved food storage ability as compared with general plastic products, their efficacy is insufficient. In addition, Korean Patent Publication No. 2000-0032538 discloses an antibacterial bioceramic packaging film A bioceramics packaging film was prepared by mixing iron oxide, manganese oxide, zeolite, ceramics powder, paraffin, terpin resin, silicone oil, and the like in polyethylene. However, although this exhibited somewhat improved preservation ability of food, Its efficacy was insufficient.

Bamboo refers to Phyllostachys bambusoides Sieb, Phyllostachys nigra Munro, Phyllostachys pubescens Mazel, and copepods belonging to Bambusaceae, which is a deciduous tree, It is known that the inorganic compounds such as fat, carbohydrate, phosphorus, calcium and iron have anti-inflammatory effects.

The hinoki cypress is a Japanese Chamaecyparis obtusa Sieb belonging to Cupressaceae, and contains chamaecypanones A and B, obtulignolide and isootobanone ) And lignans components (Kuo, Y.-H. et al, (September 2001). "Three novels and one new lignan, chamaecypanones A, B, obtulignolide and isootobanone from the heartwood of Chamaecyparis obtusa var. Tetrahedron Lett. 42 (38): 67316735. doi: 10.1016 / S0040-4039 (01) 01272-2) and sciadopitysin, ginkgetin, isoginkgetin, Podocarpus flavone B, 7,7 "-O-dimethylamentoflavone, bilobetin, podocarpus flavone A, 7-O-methylamentoflavone, Containing a biflavone component such as amentoflavone and hinokiflavone, Known and (Krauze-Baranowska, M .; Pobłocka, L .; El-Hela, A. A. (SeptemberOctober 2005) "Biflavones from Chamaecyparis obtusa" Z. Naturforsch C 60 (910):... 679685. PMID 16320608)

Propolice is a substance made by using various bees' various beeswax collected from various trees such as fir and willow and the secretion of beeswax and its beeswax in pollen collected from flowers. , 50 to 55% of resin (resin), 25 to 35% of beeswax, 10% of oily components such as essential oil, 5% of pollen, various organic substances and 5% And flavonoids (flavonoids, flavonols, etc.) are known to be the main ingredients. Propolis plays a major role in antiinflammation, antimicrobial activity, and improvement of blood circulation. (Naver Knowledge Backgammon, Power Food Superfood, Blue happiness)

     However, in any of the above-mentioned documents, the antimicrobial film for food preservation for freshness maintenance and bacterial decay is characterized by being made of a synthetic resin for food packaging, in which at least one herbal medicine extract selected from the group consisting of bamboo, cottonwood and propolis is added as an antimicrobial additive. None of the methods for preparing the same are taught or disclosed.

Accordingly, the present inventor has found an antibacterial film for food packaging containing one or more herbal medicine extracts selected from the group consisting of novel bamboo, whitening wood and propolis, and a method for producing the same, and the antibacterial film for food packaging prepared by the above- The present inventors have completed the present invention by confirming that the antimicrobial film for food packaging is provided by solving the problems in the prior art by confirming that the freshness of the food can be maintained for a long time and the antimicrobial property capable of suppressing bacterial decay is obtained through the sensory test method .

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems, and an object of the present invention is to provide a method for preventing microbial growth of foods, which can maintain the freshness of foods for a long time by advancing the degree of corruption very slowly, And to provide a freshness-retaining and bacterial anti-decay antibacterial film produced therefrom.

Accordingly, the present invention provides an antimicrobial film for freshness maintenance and bacterial decay prevention food packaging, which is produced from a synthetic resin for food packaging, in which at least one herbal medicine extract selected from the group consisting of bamboo, cottonwood and propolis is added as an antimicrobial additive do.

The herbal medicine extract as defined herein preferably comprises a blend consisting of bamboo, cottonwood and propolis, more preferably a blend weight ratio (w / w) of bamboo, cottonwood and propolis of 0.01-100: 0.01-1 (W / w), more preferably 1-30 parts by weight, and most preferably 1-30 parts by weight (w / w) ). ≪ / RTI >

The extract as defined herein is an extract which is soluble in water, a C ₁ to C ₄ lower alcohol or a mixed solvent thereof, preferably water or a water and ethanol mixed solvent, more preferably a water and ethanol mixed solvent, more specifically (W / v) weight of the sample, preferably 1 to 10 times (w / v) weight of the sample, by washing the bamboo, whitening wood and propolis, , Methanol, ethanol or a mixed solvent thereof, preferably water and an ethanol mixed solvent at 50 to 120 ° C, preferably about 80 to 100 ° C for 1 hour to 5 hours, preferably 2 hours to 4 hours A first step of performing a cold extraction method or an ultrasonic extraction method, preferably a hot water extraction method; A second step of filtering the extract obtained in the above step with a filter paper to obtain a filtrate; A third step of subjecting the filtrate to lyophilization, room temperature drying or hot air drying, preferably lyophilization to obtain individual herbal extracts in a dry state; The dry single herbal medicine extract is blended in a blending ratio of 0.01-100: 0.01-1: 1-100 parts by weight, more preferably 0.1-50: 0.1-1: 1-50 parts by weight of bamboo, (W / w), and still more preferably 1-30: 1: 1-30 parts by weight of the herb extract of the present invention.

Herb medicine extracts such as bamboo, cottonwood, and propolis are antimicrobial additives exhibiting strong antimicrobial activity. They are used in an amount of 0.01 to 20.0% by weight, preferably 0.1 to 10.0% by weight, more preferably 0.1 to 10.0% by weight, 1 to 5.0% by weight.

When the content of the synthetic resin usable in the present invention exceeds 99.9% by weight or the content of the antimicrobial additive is less than 0.01% by weight, the antibacterial activity of the film for food packaging is lowered because the content of the antimicrobial additive is relatively smaller than the content of the synthetic resin When the content of the synthetic resin is less than 80.0 wt% or the content of the antimicrobial additive is more than 20.0 wt%, the antibacterial activity of the film for food packaging is improved. However, since the bonding force between the chains of the synthetic resin is lowered as the content of the antimicrobial additive is increased, There is a problem that the stretching force of the wrapping film is insufficient and the tensile strength, which is a mechanical property, is lowered and the economical efficiency is lowered.

The synthetic resin for food packaging as defined herein includes but is not limited to polyethylene (PE), polypropylene (PP), polyamide (PA), polycarbonate (PC), polyvinyl chloride polyvinylchloride, polyvinylidene chloride (PVDC) resin, polyurethane, acrylic polymer, polystyrene (PS), polyethylene terephthalate (PET) Polyethylene (PE), polypropylene (PP), and polyethylene terephthalate (PET) -based synthetic resins such as ethylene-vinyl acetate (EVAL) At least one synthetic resin selected from the group consisting of synthetic resins, more preferably linear low density poly More preferably at least one synthetic resin selected from the group consisting of linear low-density polyethylene and low-density polyethylene (LPE) -based synthetic resins, more preferably 40.0 to 90.0 weight percent of a linear low-density polyethylene resin %, And 10.0 to 60.0% by weight of low density polyethylene (LPE) polyethylene.

The present invention is a method for uniformly dispersing and distributing antimicrobial additive particles in a film for food packaging, wherein the microparticles of the synthetic resin film are preliminarily mixed with the dry powder particles of the antibacterial additive evenly.

As described above, since the preliminarily mixed primary raw materials are mixed with the synthetic resin at a certain ratio and extruded according to a general film production method to produce a film for food packaging, the size of the film for food packaging Not only industrial mass production is possible but also a film excellent in stretching force and tensile strength can be maintained.

The synthetic resin used in the present invention may contain known additives such as heat stabilizers, antioxidants, light stabilizers, nucleating agents, flame retardants, pigments, dyes, curing agents, polycondensation catalysts, dispersants, electrostatic agents, , A nucleating agent, an anti-blocking agent and the like may be added within a range not to impair the effects of the present invention.

The molecular weight of the synthetic resin used in the composition of the present invention is 1,000 to 100,000 g / mol, preferably 5,000 to 50,000 g / mol. The melting point of the synthetic resin is usually 150 to 185 ° C, preferably 155 to 180 ° C, and the glass transition temperature of the synthetic resin is usually 40 to 120 ° C, preferably 50 to 90 ° C.

In order to produce a polyethylene film which is the base layer of the polyethylene antimicrobial film according to the present invention, the polyethylene resin is dried under a vacuum condition at a temperature of 150 DEG C for 3 hours to remove moisture inside. The dried material is melted / mixed / transferred through an extruder heated to 270 ° C to 290 ° C. Preferable conditions for the extruder to be used at this time are: a small variation in the amount of extrusion; a good kneading and dispersing ability; a uniform extrusion capable; a low temperature extrusion capable of not causing deterioration of the resin properties; It is preferable that bulk extrusion capable of satisfying the above requirements is possible. If the extrusion amount exceeds 1 ton per hour, the increase of the resin temperature becomes large in the single-screw extruder, deteriorates the resin and deteriorates the moldability, thereby deteriorating the quality. In this case, a tandem extruder is used.

Generally, in the polyethylene film, an electrostatic pinning method is used in which an electrostatic charge is applied to a molten resin to adhere to a cooling roll. In the production of the polyethylene film, generally a continuous stretching method is adopted, and stretching is performed in the order of longitudinal / transverse direction. In the longitudinal stretching step, the master is heated in a roll group to stretch the main yarn in the longitudinal direction between the other rolls, and the molecular orientation is imparted to improve the mechanical properties in the longitudinal direction. The preheating part absorbs the thermal expansion of the sheet and prevents slippage. In the stretching part, elongation is suppressed to suppress the necking in order to maintain the uniformity of the physical properties in the width direction and the precision of the thickness.

Since the longitudinal stretching temperature of the polyethylene film is 90 to 110 ° C, an infrared heater is used for the stretching part or a non-sticking roll is used because it can be adhered to the chrome plating roll in the stretching temperature range. Further, in order to increase the molding speed, it is possible to employ a multi-step method. In the transverse stretching step, the sheet is clamped at its ends with clips, and the sheet is uniformly preheated by the hot air in the upper and lower sides and is stretched in the width direction at a constant magnification according to the clip guide rails set in a predetermined pattern. The heating chamber is structured so that hot air contacting the film is uniformly heated and the independence of each chamber can be maintained. The film coming from the transverse stretching machine is taken, and both edges are trimmed and wound. With the widening of the film and the higher production speed, the role of the winding machine is very important. The polyethylene film is preferably subjected to corona treatment on the surface thereof in order to improve the adhesion, and is preferably a biaxially stretched film for the purpose of improving mechanical properties.

The present invention also relates to a process for producing an antimicrobial film for food storage, which can be used as a food packaging material or an antimicrobial material which requires freshness maintenance by improving antimicrobial properties, transparency and liveness while maintaining the basic properties of conventional synthetic resins for food storage ≪ / RTI >

Specifically, the present invention provides a method for preparing a herbal medicine comprising: (1) preparing a powdery sample of at least one herbal medicine extract selected from the group consisting of bamboo, cottonwood and propolis; (2) The powdery sample is added in an amount of 0.01 to 20.0% by weight, preferably 0.1 to 10.0% by weight, more preferably 1 to 5.0% by weight, based on 100% by weight of synthetic resin for food packaging, and stirred to prepare a resin mixture A second step; (3) a third step of adding a two-stage mixture to a hopper of an extrusion molding machine and heating and melting the cylinder and the dice while stirring to produce a film extrudate in the form of a bubble-forming tube; ; (4) cooling the film extrudate with a cooling chiller; (5) removing air in the film extrudate to produce a flat plate-like master batch flat plate film; (6) a step of corona treatment or plasma treatment so that the flat film can be printed or dried, and a sixth step of producing an antibacterial film product for food storage, To provide a manufacturing method for manufacturing a film product.

In the first step of the manufacturing process,

(W / v) weight of the sample, preferably 1 to 10 times (w / v) weight of the sample, by washing the dried herbal materials such as bamboo, v) by weight of water, methanol, ethanol or a mixed solvent thereof, preferably water and ethanol mixed solvent at 50 to 120 ° C, A first step of performing a hot water extraction method, a cold extraction method or an ultrasonic extraction method, preferably a hot water extraction method, for 4 hours; A second step of filtering the extract obtained in the above step with a filter paper to obtain a filtrate; A third step of subjecting the filtrate to lyophilization, room temperature drying or hot air drying, preferably lyophilization to obtain individual herbal extracts in a dry state; The blending ratio of each individual herb medicine extract in the dry state or the combination ratio of bamboo, whiteness and propolis is 0.01-100: 0.01-1: 1-100 parts by weight, more preferably 0.1-50: 0.1-1: 1- 50 to 50 parts by weight (w / w), and even more preferably 1-30: 1: 1-30 parts by weight.

In the second step of the manufacturing process,

Further, as an additive which can be added to the synthetic resin for food packaging and constitute a master batch, a plasticizer such as dioctyl adipate (DOA) or dioctyl phthalate (DOP, Dioctyl Phthalate) A heat stabilizer such as a bis organosilicate stabilizer such as di-n-octyltin bis (isooctyl thioglycolic acid ester) which can act as a lubricant such as soybean oil or prevent thermal decomposition can be added.

In the third step of the manufacturing process,

Cylinders and dice are usually 150 to 185 DEG C, preferably 155 to 180 DEG C, which is the melting point of the synthetic resin. The glass transition temperature of the synthetic resin is usually 40 to 120 DEG C, preferably 50 to 90 DEG C And heating.

In a fourth step of the manufacturing process,

The cooling chiller is characterized by using a cooling chiller using gas, a water-cooling chiller using water, or an air chiller using air, preferably air chiller using air.

In a sixth step of the manufacturing process,

The corona treatment method refers to a surface treatment method by high frequency electric discharge, and the plasma treatment method refers to a surface treatment method in which Ar (argon) or N2 (nitrogen) gas is injected under vacuum or atmospheric pressure and plasma is generated by electric discharge.

The antimicrobial film for food packaging prepared according to the above production method has an antibacterial test and a sensory test to show that the freshness of the food can be maintained for a longer period of time as compared with the conventional antimicrobial food film and exhibits more excellent antibacterial properties for inhibiting bacterial decay It is possible to provide an antimicrobial film for food packaging which solves the problems in the prior art.

Accordingly, the present invention provides an antibacterial film product for food packaging excellent in antibacterial activity produced by the above production method.

Hereinafter, the specific structure of the present invention will be described in detail with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to the description of these embodiments.

The antimicrobial film for food packaging for freshness and bacterial decay prevention, which is produced from a synthetic resin for food packaging, which is obtained by adding at least one herbal medicine extract selected from the group consisting of bamboo, whitening wood and propolis of the present invention as an antimicrobial additive, The antibacterial film for food packaging was solved by confirming that the freshness of the food can be maintained for a longer period of time than the conventional antimicrobial food film and the antimicrobial property which can inhibit bacterial decay is confirmed through the sensory test method, Available.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a diagram showing a manufacturing process for producing the antimicrobial film of the present invention.

Hereinafter, the present invention will be described in detail with reference to the following examples and examples.

However, the following examples and experimental examples are illustrative of the present invention, and the content of the present invention is not limited by the following examples, reference examples and examples.

Example  1. Mixed herbal extract Manufacturing example

(1) Preparation of bamboo extract

- 1 kg of dried leaves (Kyungdong market, Seoul) were pulverized and pulverized, and then 6 liters of 95% ethanol was added and ultrasonically extracted three times for 90 minutes, followed by filtration and concentration under reduced pressure. The concentrated filtrate was lyophilized in a freeze dryer (ScanVac, Labogene) under reduced pressure to obtain 70 g (7.0%) of an extract powder (hereinafter referred to as PBE)

(2) Preparation of extracts

- 5 kg of 95% ethanol was added to 1 kg of dried white rice bran (Kyungdong market, Seoul) and ultrasonically extracted twice for 90 minutes, filtered and concentrated under reduced pressure. The concentrated filtrate was lyophilized with a freeze dryer (ScanVac, Labogene) under reduced pressure to obtain 54.0 g (5.4%) of an extract powder (hereinafter referred to as COE)

(3) Preparation of propolis extract

- 5 kg of 95% ethanol was added to 10 kg of dried propolis ( Kyungdong market, Seoul), sonicated twice for 90 minutes, filtered, and concentrated under reduced pressure. The concentrated filtrate was lyophilized in a freeze dryer (ScanVac, Labogene) under reduced pressure to obtain 600 g (6.0%) of an extract powder (hereinafter referred to as POE)

(4) Mixed herbal medicine extract Manufacturing example  One

- 10 mg of dried bamboo, unbleached wood and propolis extract obtained in the above step were respectively weighed and mixed with a mixer (Vortex genie-2, scientific industries, USA) to obtain a mixed herbal extract (hereinafter referred to as "bnkb1" The next step was used as an antimicrobial additive.

(5) Mixed herbal medicine extract Manufacturing example  2

- The dried bamboo (10 mg), Prunus mume (20 mg) and propolis extract (10 mg) were each weighed and mixed with a mixer (Vortex genie-2, scientific industries, USA) to obtain a mixed herbal extract (hereinafter referred to as "bnkb2" ) Was used as an antimicrobial additive in the next step.

Example  2-8. Production of antibacterial film (Fig. 1)

(1) Feeding and melting step

The antimicrobial antibacterial film according to the present invention was tested for its antibacterial antibacterial film as shown in the following Table 1 at a stirring speed of 2,000 rpm at room temperature using a high-speed powder mixer (HKSM-600, Korean Powder System) After mixing for 10 minutes with stirring, the mixed composition was introduced into a hopper of an extrusion molding machine (HSE 45, Korea Em, length 2.8 m, die hole diameter 2 mm equipped with a single screw having a diameter of 10 cm) and the cylinder and die were heated to 150 5 Melted by heating, and extruded to obtain an extrudate in the form of a tube in the form of a bubble.

Single or mixed herbal extracts and various synthetic resins * Example 2 Example 3  Example 4  Example 5 Example 6 Example 7 Example 8 Synthetic resin
(100%) PE series resin
The mixture (LLDPE: 70% + LDPE: 30%) PE series resin
mixture
(LLDPE: 50% + LDPE: 50%) PP series resin
(weight) PET-based resin
(weight) PE series resin mixture (LLDPE: 70% + LDPE: 30%) PP series resin
(weight) PET-based resin
(weight) additive bnka1
(One%) bnka2
(2%) bnka1
(5%) bnka2
(2%) PBE
(2%) COE
(2%) POE
(2%) * PE; (polyethylene), PP; (polypropylene), PET; polyethylene terephthalate;
LLDPE: Linear low-density polyethylene, LDPE; 도동

(2) cooling and plate forming steps

After the extrudate of the above step is cooled in a cooling chiller (CHL-003, negative freezing industry) and then cut to produce a polyethylene master batch, the tubular product is air- .

(3) Corona treatment and product processing steps

Corona processing was performed with a corona apparatus (AGF-010, Dyne C & T) so that printing or drying operation could be performed on the flat film of the above-mentioned step, and then processed into a final product through winding and cutting operations.

Comparative Example 1. Preparation of comparative film (1)

In the production of the film described in Example 2-8, the same process as that of the film production method described in Example 2-8 was carried out, and the PE-based resin mixture (LLDPE: 70% + LDPE: 30%) (Polyethylene) series comparative film (1) was prepared and used for comparison in the following experimental examples.

Comparative Example 2. Preparation of comparative film (2)

In the production of the film described in Example 2-8, the same processes as those of the film production method described in Example 2-8 were carried out to produce a PP (polypropylene) type comparative film (2) without the additives described in Table 1, The sample was used for comparison.

Comparative Example 3. Production of comparative film (3)

In the production of the films described in Examples 2-8, the same processes as those of the film production method described in Example 2-8 were carried out to obtain PET without the additives shown in Table 1; ( 3) composed of polyethylene terephthalate was prepared and used for comparison in the following experimental examples.

Experimental Example 1. Antibacterial Experiment

(Bae JH, Korean J. Nutr., 36, 147-153) using an antimicrobial film according to the above-described example,

1-1. Bacterial culture

Propionibacterium As a liquid culture medium for acnes culture, gifu anaerobic medium (GAM) was used, Escherichia coli and Staphylococcus Nutrient broth (NB) was used as a liquid medium for epidermidis , and tryptic soy broth (TSB) was used as a liquid medium for Staphylococcus aureus . The solid medium was used in this experiment by adding agar to the liquid medium. Propionibacterium acnes were incubated in CO ₂ incubator and other strains were incubated at 37 ° C in BOD incubator.

1-2. Measurement of growth zone (Clear zone)

The antimicrobial activity was measured by paper disc method ^{8 )} . Namely, each strain cultured on a plate medium was inoculated into 10 mL of the liquid medium, 0.1 mL of the bacterial solution, and cultured for 3 to 6 hours. After culturing for 3 to 6 hours, 1 plate The cells were inoculated at a concentration of about 1 x 10 < ⁷ > cells and uniformly plated with a sterilized cotton swab. After sterilized filter paper discs (8 mm, Tokyo, Japan) were placed on a solid plate medium, the samples were absorbed to concentrations of 0.05 mL / disc and incubated at 37 ° C for 18-24 hours to obtain clear zone Diameter was measured.

The comparative films of Comparative Examples 1 to 3 exhibited relatively low antibacterial activity, whereas the antibacterial food films of Examples 2 to 8 were staphylococcus aureus , Escherichia coli and Staphylococcus As a result of observation of clear zone formation against epidermidis , it was found that the antimicrobial activity was strong as shown in Table 2.

Antimicrobial activity on several microorganisms Strains Clear Zone on Plate  ( mm ) ^One) Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Comparative Example 1 Comparative Example 2 Comparative Example 3 Propioni bacterium acnes KCTC 3314 12 12 18 13 10 11 12 9 9 11 Escherichia coli
KCTC 1039 15 15 20 13 13 14 12 11 11 12 Staphylococcus epidermidis KCTC 1917 17 16 23 15 15 15 14 12 10 11 Staphylococcus
aureus KCTC 1621 19 22 21 17 16 18 15 13 15 13 1) Diameter
2) Example 2: PE-based resin mixture (LLDPE: 70% + LDPE: 30%) + bnkb1 (1%
3) Example 3: PE-based resin mixture (LLDPE: 50% + LDPE: 50%) + bnkb2 (2%
4) Example 4: PP series resin + bnkb1 (5%)
5) Example 5: PET-based resin + bnkb2 (2%)

Experimental Example 2: Food preservation experiment (fish food)

Experimental Example 2 is to confirm the effect of extending the shelf-life of food using the film according to the above-described embodiment. For this purpose, the effects of mackerel fillet (Fillet) on microbial growth during storage were investigated. The mackerel fillets having good lines were closely packed with the films according to Examples 2 to 8, packed, and stored at 10 캜 to examine the growth pattern of the microorganisms over time. The number of initial microorganisms was 1.3 x E2 / g. Here, "E2" represents "10 ² ".

5 days 10 days 15th 20 days Example 2 2.7 x E2 / g 1.5 x E3 / g 2.5 x E5 / g 1.2 x E7 / g Example 3 1.3 x E2 / g 3.4 x E3 / g 4.2 x E5 / g 2.5 x E7 / g Example 4 3.5 x E2 / g 2.9 x E3 / g 6.9 x E5 / g 3.7 x E7 / g Example 5 4.3 x E2 / g 4.5 x E4 / g 8.1 x E6 / g 9.2 x E9 / g Example 6 6.7 x E2 / g 1.3 x E4 / g 6.5 x E6 / g 8.3 x E8 / g Example 7 7.9 x E2 / g 3.7 x E4 / g 1.8 x E6 / g 6.3 x E8 / g Example 8 6.8 x E2 / g 3.0 x E4 / g 9.2 x E6 / g 1.9 x E9 / g Comparative Example 1 2.2 x E3 / g 1.3 x E6 / g 6.5 x E8 / g 8.3 x E13 / g Comparative Example 2 1.3 x E3 / g 4.2 x E6 / g 3.5 x E8 / g 4.1 x E13 / g Comparative Example 3 2.4 x E3 / g 3.9 x E5 / g 1.1 x E8 / g 7.3 x E10 / g

As can be seen from Table 3, in the case of the sample packed with the antibacterial film for food packaging according to the embodiment of the present invention, the number of microorganisms reached 2.5 x E5 / g to 8.1 x E6 / g after 15 days of storage, It can be seen that the antimicrobial film for food packaging according to the present invention exhibits an excellent microorganism growth inhibition effect as compared with a film using only general PE, PP and PET films according to the comparative examples.

Experimental Example 3: Food preservation test (fruit food)

In Experimental Example 3, commercially available drop tomatoes were packed in the films according to the above-described Examples and Comparative Examples, and stored in a thermostatic chamber at 20 ° C and 30 ° C for 5 days, 7 days, and 10 days, The degree of rotting of the tomatoes was visually observed and the results are shown in Table 4 below. In Table 4, the degree of corruption is visually confirmed to indicate the degree of corruption in Table 4, "O" means a good state, " DELTA "means slightly corrupted state, and" X "means" corrupt state.

5 days  7 days  10 days 20 ℃ 30 ℃ 20 ℃ 30 ℃ 20 ℃ 30 ℃ Example 2  ○  ○ ○ ○ ○ △ Example 3  ○  ○ ○ ○ ○ △ Example 4  ○  ○ ○ ○ ○ △ Example 5  ○  ○ ○ ○ ○ △ Example 6  ○  ○ ○ △ △ △ Example 7  ○  ○ ○ △ △ △ Example 8  ○  ○ ○ ○ ○ △ Comparative Example 1  △  X X X X X Comparative Example 2  △  △ X X X X Comparative Example 3  △  X X X X X

As can be seen from the above Table 4, in the case of the drop tomato which was packed in the antimicrobial film for food packaging according to the embodiment of the present invention and kept in a thermostatic chamber at 20 ° C for about 7 days, the freshness was maintained even after about 7 days And it was found that it was slightly corrupted when 10 days passed. In case of the drop tomato preserved for about 5 days in a thermostat of 30 ° C, freshness was maintained even after about 5 days, and when about 7 days passed It can be seen that the film used as the general film without the additive according to the comparative example shows much corruption in the thermostatic chamber at 20 ° C and 30 ° C within five days.

Therefore, as shown in Tables 1 to 4, the antimicrobial film for food packaging according to the present invention has excellent microbial growth inhibiting effect on food and can proceed the degree of corruption very slowly, so that freshness of food can be maintained for a long time, It can be understood that it has an excellent effect of suppressing corruption.

Claims (11)

Wherein the antimicrobial film is prepared from a synthetic resin for food packaging containing at least one herbal medicine extract selected from the group consisting of bamboo, cottonwood, and propolis as an antimicrobial additive. The method according to claim 1,
Wherein the herbal medicine extract is a combination of bamboo, cottonwood, and propolis. 3. The method of claim 2,
Wherein the weight ratio (w / w) of bamboo, cottonwood and propolis is 0.01-100: 0.01-1: 1-100 parts by weight (w / w). The method according to claim 1,
Wherein the extract is an extract which is soluble in water, C ₁ to C ₄ lower alcohols or a mixed solvent thereof. The method according to claim 1,
Wherein the antibacterial additive is added in an amount of 0.01 to 20.0% by weight based on 100% by weight of synthetic resin for food packaging. The method according to claim 1,
The synthetic resin for food packaging may be selected from the group consisting of polyethylene (PE), polypropylene (PP), polyamide (PA), polycarbonate (PC), polyvinylchloride (PVC) Polyvinylidene chloride (PVDC) resin, polyurethane, acrylic polymer, polystyrene (PS), polyethylene terephthalate (PET), and ethylene vinyl acetate (EVAl) Wherein the synthetic resin is at least one synthetic resin selected from the group consisting of synthetic resins based on polylactic acid. The method according to claim 1,
Wherein the synthetic resin for food packaging is at least one synthetic resin selected from the group consisting of polyethylene (PE), polypropylene (PP), and polyethylene terephthalate (PET) synthetic resin. (1) a first step of preparing a powdery sample of at least one herbal medicine extract selected from the group consisting of bamboo, cottonwood and propolis; (2) a second step of adding the powdery sample in an amount of 0.01 to 20.0% by weight based on 100% by weight of synthetic resin for food packaging and stirring to prepare a resin mixture; (3) a third step of adding a two-stage mixture to a hopper of an extrusion molding machine and heating and melting the cylinder and the dice while stirring to produce a film extrudate in the form of a bubble-forming tube; ; (4) cooling the film extrudate with a cooling chiller; (5) removing air in the film extrudate to produce a flat plate-like master batch flat plate film; (6) A method for producing an antimicrobial film product for foodstuff, comprising the steps of: performing corona treatment or plasma treatment to enable printing or drying on the flat film; and A method of manufacturing a film product. 9. The method of claim 8,
In the third step of the manufacturing process, the cylinder and the dice are heated to a temperature of usually from 150 to 185 캜, which is the melting point of the synthetic resin, and the glass transition temperature of the synthetic resin is from 40 to 120 캜. Way. 9. The method of claim 8,
Wherein in the fourth step of the manufacturing process, the cooling chiller is a cooling chiller using gas, a water-cooling chiller using water, or an air chiller using air. An antimicrobial film product for food packaging, wherein the production method of claim 8 is produced with excellent antibacterial activity.

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