KR20160059606A - Composition for preventing or treating cavity and periodontal disease comprising Robinia pseudoacacia extract or its fraction as effective component - Google Patents

Abstract

The present invention relates to an antibacterial composition against streptococcus or Porphyromonas gingivalis, comprising a Robinia pseudoacacia extract or fractions thereof as an active component; a pharmaceutical composition comprising a Robinia pseudoacacia extract or fractions thereof as an active component for preventing or treating cavity or periodontal diseases; and a healt food composition comprising a Robinia pseudoacacia extract or fractions thereof as an active component for preventing or treating cavity or periodontal diseases.

Description

[TECHNICAL FIELD] The present invention relates to a composition for preventing or treating periodontal diseases and cavities comprising an extract of Robinia pseudoacacia extract or a fraction thereof as an active ingredient,

The present invention relates to a composition for prevention or treatment of periodontal disease and cavities comprising an extract of the Arabidopsis thaliana or its fractions as an active ingredient. More particularly, the present invention relates to a composition for preventing or treating periodontal disease or periodontal disease, A pharmaceutical composition for preventing or treating periodontal disease or periodontal disease comprising an antimicrobial composition, an extract of Robinia pseudo-acacia var. Umbraculifera or a fraction thereof as an active ingredient, and a composition for preventing or ameliorating cavities or periodontal disease Functional food composition.

A tooth is a tooth that is worm-wormed. The tooth's hard tissue is eroded and it is called a dental caries. Tooth decay is an infectious bacterial disease that causes part of the hard calcified tissue of the tooth to dissolve and break down and ultimately lose teeth. It is the most common periodontal disease.

Periodontitis is a chronic inflammatory disease caused by bacteria and toxins in the microbial plaque, which is caused by inflammation of the surrounding tissues of the teeth. Periodontal tissue that supports the teeth is gradually destroyed, causing the tooth to shake and eventually fall out. Periodontitis is an idiom and is often referred to as a periodontal disease. It is also called periodontal disease or periodontal disease. When periodontal disease occurs, the gingiva falls and the tooth root appears and the symptoms of syenin appear.

Dental medicine Various oral diseases including tooth decay, gingivitis and periodontal disease can be caused by various causes. The cause is divided into systemic cause and local cause, and the most important cause is local cause. The most important local cause is bacteria that reside in the mouth, that is, pathogenic microorganisms.

It is known in the human oral cavity that about 300 kinds of microorganisms are resident on the surface of the teeth, between the teeth and gums of the root portion, and on the surface of the tongue, and the presence of such microorganisms, Phenomenon. However, if proper oral hygiene activities are not performed, various oral diseases such as tooth decay, halitosis, gingivitis and periodontal disease may be caused by pathogenic microorganisms among the above-mentioned microorganisms, and in severe cases, teeth may be lost.

More specifically, a pathogenic microorganism resident in the oral cavity attaches to the surface of the tooth and forms a bacterial population called a plaque after a few hours. At first, a pathogenic microorganism attaches However, as progressive progression occurs, plaque is formed on the surface of the tooth under the gingiva. When the plaque is formed on the tooth surface, the pathogenic microorganism produces an acid by using the sugar introduced into the oral cavity, and this acid may cause tooth decay by demineralizing the main ingredient of the tooth.

Examples of the microorganisms that play the most important role in inducing oral diseases include Streptococcus mutans , Porphyromonas gingivalis , and the like. In particular, Streptococcus mutans is a causative organ of tooth decay and causes various oral diseases.

Conventionally, various kinds of antimicrobial agents including antibiotics having sterilization and bacteriostatic action against the microorganisms have been developed as inhibitors and remedies for cavity, periodontal disease or gingivitis. Conventional materials include fluorine and chlorine-containing compounds such as sodium copper chlorophyllin, sodium fluoride or benzethonium chloride, benzoic acid, , Allantoin, tocopheryl acetate, antiplasmic agents or antibiotics, and the like. However, in the case of the fluorine-chlorine-containing compound, safety of the human body is problematic. Antibiotics can cause systemic side effects, including diarrhea and vomiting, in the whole body, It is difficult to use it for a long period of time because it can induce homozygosity, so that it can be used only as a therapeutic agent. In addition, in the case of treatment by surgical treatment or mechanical therapy, there is a disadvantage that treatment is not easy.

Therefore, it is easy to prevent or treat as compared with surgical therapy or mechanical therapy, and there is no problem of tolerance or stability such as antibiotics even when used for a long time, and there is no problem of side effects such as a compound containing fluorine and chlorine components There is an urgent need to develop a composition for the prevention or treatment of oral diseases derived from natural materials, and there is an increasing demand for developing a composition capable of preventing or treating oral diseases from natural materials.

Korean Patent No. 1280868 discloses a pharmaceutical composition and a food composition for preventing or treating periodontal diseases and cavities containing an extract of YAKON as an active ingredient, Korean Patent Laid-Open No. 2011-0103267 discloses a composition comprising &Quot; oral cavity composition for prevention and treatment of periodontal disease ", which is an effective ingredient, is disclosed. However, no description has been made on a tooth cavity and a composition for the prevention or treatment of periodontal disease containing the extract of the present invention or its fraction as an active ingredient.

SUMMARY OF THE INVENTION The present invention has been made in view of the above needs, and it is an object of the present invention to provide an antibacterial and antimicrobial agent for preventing and treating pathogens such as Streptococcus mutans, which is a carrot bacteria, and Porphyromonas gingivalis, Activity, the present invention has been completed.

In order to solve the above-described problems, the present invention provides a composition for antibacterial activity against a decubitus bacteria or periodontal bacteria containing an extract of the Arabidopsis thaliana or a fraction thereof as an active ingredient.

The present invention also provides a pharmaceutical composition for prevention or treatment of tooth decay or periodontal disease, which comprises an extract of Saplings of Aspergillus or fractions thereof as an active ingredient.

Further, the present invention provides a health functional food composition for preventing or ameliorating tooth decay or periodontal disease, which comprises an extract from Sapporo tree or a fraction thereof as an active ingredient.

Since the composition containing the extract of Robinia pseudo-acacia var. Umbraculifera or its fractions according to the present invention shows excellent antimicrobial activity against Streptococcus mutans bacteria, which are carrot bacteria and Porphyromonas gingivalis bacteria, which are periodontal bacteria, And tooth decay bacteria, and a health functional food composition for preventing or ameliorating tooth decay and periodontal disease.

Fig. 1 shows the results of inhibiting the growth inhibition of Streptococcus mutans strains of the methanol extract of A. thunbergii and its fractions. MeOH ex, methanol extract; Butanol fractions of BuOH fr, methanol extract; CHCl ₃ fr, chloroform fraction of methanol extract; EtOAc fr, ethyl acetate fraction of methanol extract; Hexane fr, hexane fraction of methanol extract.
Fig. 2 shows the results of inhibiting the growth inhibition rate of P. falciparum methanol extract and fractions thereof against P. fluorescens. MeOH ex, methanol extract; Butanol fractions of BuOH fr, methanol extract; CHCl ₃ fr, chloroform fraction of methanol extract; EtOAc fr, ethyl acetate fraction of methanol extract; Hexane fr, hexane fraction of methanol extract.

In order to accomplish the object of the present invention, the present invention provides a composition for antimicrobial treatment against a decubitus or periodontal bacteria containing an extract of Saplings of Aspergillus or fractions thereof as an active ingredient.

In the antimicrobial composition according to an embodiment of the present invention, the cavity can be Streptococcus mutans , Streptococcus sobrinus and Lactobacillus salivarius , But are not limited to, Streptococcus mutans , and the periodontal bacteria may be selected from the group consisting of Porphyromonas gingivalis , Tannerella forsythia , Fusobacterium, nucleatum , Actinobacillus actinomycetemcomitans , and the like, preferably, but not limited to, Porphyromonas gingivalis .

In the antimicrobial composition according to one embodiment of the present invention, the Abacus crispa extract may be an extract using leaves or stem parts of the plant, but is not limited thereto.

The extract may be water, methanol, ethanol, propanol, butanol or a mixed solvent thereof. Preferably, the extract may be methanol extract of dried bamboo trees. Specifically, Dried at a temperature of 30 to 50 ° C for 2 to 4 days, 75 to 85% of methanol was added to the dry weight of 400 to 600 g, extracted with an ultrasonic apparatus, and filtered have.

In the antimicrobial composition according to one embodiment of the present invention, the fractions of the above-described extracts of the family Acacia var. Umbraculifera are prepared by mixing 75 to 85% of methanol with 400 to 600 g of dried acacia tree, extracting them using an ultrasonic device, filtering, The methanol extract from which the solvent has been removed is dissolved in water, and then chloroform or hexane (n-hexane) is added.

The present invention also provides a pharmaceutical composition for prevention or treatment of tooth decay or periodontal disease, which comprises an extract of Saplings of Aspergillus or fractions thereof as an active ingredient.

The term 'periodontal disease' is often referred to as a style, depending on the degree of disease gingivitis (gingivitis) and periodontitis (periodontitis) is divided into. It is a relatively light and fast-acting periodontal disease called gingivitis that is limited to gingiva or soft tissue, and it is called periodontitis when the inflammation progresses to the gums and the gums.

The extracts or fractions of the present invention may comprise suitable carriers, excipients and diluents conventionally used in the manufacture of pharmaceutical compositions.

The pharmaceutical dosage forms of the extracts or fractions of the present invention may be used in the form of their pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds as well as in suitable aggregates.

The extract or fraction according to the present invention can be formulated in the form of powders, granules, tablets, capsules, oral preparations such as suspensions, emulsions, syrups and aerosols, external preparations, suppositories and sterilized injection solutions according to a conventional method Can be used. Examples of carriers, excipients and diluents that can be contained in the extract or fraction include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, , Methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, and the like. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose, (sucrose), lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. As a suppository base, witepsol, macrogol, tween 60, cacao paper, laurin, glycerogelatin and the like can be used.

The preferred dose of the extract or fraction of the present invention will vary depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. However, for the desired effect, the extract or fraction of the present invention is preferably administered at a daily dose of 0.0001 to 100 mg / kg, preferably 0.001 to 100 mg / kg. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The extracts or fractions of the present invention can be administered to mammals such as rats, mice, livestock, humans, and the like in a variety of routes. All modes of administration may be expected, for example, by oral administration or by injection.

Further, the present invention provides a health functional food composition for preventing or ameliorating tooth decay or periodontal disease, which comprises an extract from Sapporo tree or a fraction thereof as an active ingredient.

INDUSTRIAL APPLICABILITY The bark extract of the present invention or its fractions exhibits an excellent antimicrobial activity against Streptococcus mutans bacteria, which are carrot bacteria and Porphyromonas gingivalis bacteria, which are periodontal bacteria, and thus they are useful for preventing or improving periodontal diseases such as tooth decay or periodontal disease .

When the extract or fraction of the present invention is used as a food additive, the extract or fraction may be added as it is or may be used together with other food or food ingredients, and may be appropriately used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, the extract or fraction of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on the raw material, when the food or beverage is produced. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. Examples of the food to which the extract or fraction can be added include dairy products such as meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, Alcoholic beverages, and vitamin complexes, all of which include health functional foods in a conventional sense.

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Experimental Method

Production Example 1. Preparation of an extract of Abakashi tree

Extracts of Robinia pseudoacacia were obtained from the upper part of the roots of acacia trees. Leaves and stem parts were used. The leaves and stems of the Arabidopsis thaliana, which were confirmed by the plant taxonomist, were cut evenly into 2.54 cm by using a cutting blade and then dried in a drying oven at 40 ° C for 3 days. Using a plant weight of about 500 g, 80% methanol was extracted using an ultrasonic apparatus. The liquid was filtered through a membrane filter (0.2 μm), and the solvent was removed in a vacuum to leave a methanol extract. Through this process, cellulose in the components of the Arabidopsis thaliana is filtered through a purification process, and the extract of the Arabidopsis thaliana is a methanol extract containing mainly trace components of the Arabidopsis thaliana. All solvents used were analytical grade, purchased from Sigma-Aldrich (USA) or Merck (USA).

Production Example 2. Preparation of fractions of the extracts of Acanthopanax species

The fractions of F. acacia var. Umbraculifera were fractionated using butanol, chloroform (CHCl ₃ ), ethyl acetate or hexane (n-hexane). The fractionated sample was passed through a membrane filter (0.2 μm) before lyophilization and the solvent was removed under vacuum to leave only each fraction. These fractions were stored in a -20 ° C freezer until used. All solvents used were analytical grade, purchased from Sigma-Aldrich (USA) or Merck (USA).

Inhibition of growth of oral pathogens

The antimicrobial activity of the extracts of Acacia var. Umbraculifera and fractions against oral pathogens was examined. Briefly, 1 × 10 ⁶ cfu / ml of Streptococcus mutans UA159 (ATCC 700610) or Porphyromonas gingivalis W83 (ATCC BAA-1703) were added to 96-well plates , The methanol extract of B. thunbergii was treated at a concentration of 0.2 and 0.6 mg / ml, and the acacia tree fractions were treated at a final concentration of 0.008, 0.04 and 0.2 mg / ml, respectively. After incubation at 37 ° C for 24 hours while maintaining the anaerobic state, the growth inhibition rate of oral pathogens was analyzed.

Example 1. Measurement of antimicrobial activity against Cavendishes of the extracts and fractions of Asahi tree

The antimicrobial activities of the methanol extracts and fractions of A. thunbergii were confirmed in the methanol extracts of A. thunbergii, chloroform and hexane fractions (FIG. 1). In the case of butanol and ethyl acetate fractions, no antimicrobial activity was observed against the mildew. In the case of hexane fraction, the growth inhibition rate was over 50% at the concentration of 0.2 mg / ml. In the case of chloroform fraction, And the methanol extract showed a similar inhibition rate of growth at a concentration of 0.6 mg / ml higher than that of the hexane fraction.

Example 2: Antimicrobial activity measurement of periodontal bacteria of the extracts and fractions of Asahi tree

The antimicrobial activity of the methanol extracts and fractions of B. thunbergii on periodontal bacteria was confirmed in methanol extracts of A. thunbergii, chloroform, ethyl acetate and hexane fractions (FIG. 2). Butanol fraction showed no inhibitory activity against periodontal bacteria. In the case of chloroform, ethyl acetate and hexane fractions, the growth inhibition rate was over 80% at all at a concentration of 0.2 mg / ml. Particularly in the case of hexane fraction 0.04 < / RTI > mg / ml. Methanol extracts of A. falciparum showed higher antimicrobial activity against periodontal bacteria than the fractions.

From the above results, it was found that the methanol extract and the chloroform or hexane fraction of Robinia pseudoacacia var. Thus, it was confirmed that the extract or fraction can be used for prevention or treatment of tooth decay or periodontal disease.

Claims (7)

Robinia The present invention relates to an antimicrobial composition for a decubitus or periodontal bacteria containing an extract or a fraction thereof as an active ingredient. The antimicrobial composition according to claim 1, wherein the decidua is Streptococcus mutans . The antimicrobial composition according to claim 1, wherein the periodontal bacteria is Porphyromonas gingivalis . The antimicrobial composition according to claim 1, wherein the bark extract is a water extract of dried bark, methanol, ethanol, propanol, butanol or a mixed solvent thereof. The antimicrobial composition according to claim 1, wherein the fractions of the extract of Robinia pseudo-acacia var. Umbraculifera are chloroform or hexane fractions obtained by adding chloroform or n-hexane to a methanol extract of Robinia pseudo-acacia tree. A pharmaceutical composition for prevention or treatment of tooth decay or periodontal disease, comprising an extract of Acanthopagaceae or a fraction thereof as an active ingredient. A health functional food composition for preventing or ameliorating tooth decay or periodontal disease, which comprises an extract of Acacia var. Umbraculifera or a fraction thereof as an active ingredient.

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