KR20160031484A - Manufacturing methods of grain processed food products with improving preservation and health benefits using Korean red pine bark, and the grain processed food products thereby - Google Patents

Manufacturing methods of grain processed food products with improving preservation and health benefits using Korean red pine bark, and the grain processed food products thereby Download PDF

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KR20160031484A
KR20160031484A KR1020160028803A KR20160028803A KR20160031484A KR 20160031484 A KR20160031484 A KR 20160031484A KR 1020160028803 A KR1020160028803 A KR 1020160028803A KR 20160028803 A KR20160028803 A KR 20160028803A KR 20160031484 A KR20160031484 A KR 20160031484A
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processed food
grain processed
food
bark
rice
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KR101681475B1 (en
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한완택
한선정
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뉴트라팜주식회사
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    • A23L1/3002
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D2/00Treatment of flour or dough by adding materials thereto before or during baking
    • A21D2/08Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
    • A21D2/36Vegetable material
    • A23L1/10
    • A23L1/16
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463Organic compounds; Microorganisms; Enzymes
    • A23L3/3472Compounds of undetermined constitution obtained from animals or plants

Abstract

The present invention relates to a method for manufacturing grain processed food with improved preservability and health functionality using a pine tree bark and, specifically, to a method wherein the grain processed food is manufactured by adding pine tree bark powder to food raw materials, thereby lowering the degree of microorganism pollution and preventing the degree of rancidity, leading to the improvement in preservability, and offering functionality beneficial to the human body, such as anti-oxidative activity. According to the present invention, the pine tree bark components is added to suppress the growth of harmful microorganisms and reduce the degree of fat rancidity, thereby preserving the grain processed food for a long period of time, enhancing health functionality, such as anti-oxidative activity, due to a natural polyphenol component, and improving sensory acceptability of the grain processed food. In addition, the pine tree bark components are conveniently introduced to a conventional food process, thereby mass-producing well-being type grain processed food, leading to a very high chance of success in commercialization. Therefore, the development of various grain processed foods containing the pine tree bark components can make a great contribution to an increase in the added value of pine trees, an increase in the income of forest farms, and the development of food industry. Specifically, the natural polyphenol component of the pine tree bark exhibits efficacies of strong anti-oxidative activity, blood circulation improvement, anti-inflammation, antibacterial activity, skin beauty improvement, and endurance enhancement, and thus the grain processed food containing the natural polyphenol component is expected to be effective in the prevention and alleviation of various adult diseases, such as diabetes, hypertension, dementia, atherosclerosis, and hyperlipidemia. The active utilization of the grain processed food can contribute to the promotion of the health of consumers, and export differentiated well-being type functional food abroad, thereby greatly contributing the improvement in the international balance of payments.

Description

소나무 수피를 사용하는 보존성과 건강기능성이 강화된 곡류가공식품 제조방법{Manufacturing methods of grain processed food products with improving preservation and health benefits using Korean red pine bark, and the grain processed food products thereby}[0001] The present invention relates to a method for producing a processed food having enhanced preservability and health function using pine bark,

본 발명은 소나무 수피를 사용하는 보존성과 건강기능성이 강화된 곡류가공식품 제조방법에 관한 것으로, 구체적으로 식품원료에 소나무 수피 분말을 첨가하여 곡류가공식품을 제조함으로써 미생물 오염도를 낮추고 산패를 방지하여 보존성을 개선하고, 항산화 활성과 같은 인체에 유익한 기능성을 갖도록 하는 방법에 관한 것이다.The present invention relates to a method for producing a processed food with enhanced preservability and health function using pine bark. More specifically, the present invention relates to a method for producing a processed food by processing pine bark powder by adding a pine bark powder to a food raw material to reduce microbial contamination, And to have a beneficial function to the human body, such as antioxidant activity.

곡류가공식품은 곡류를 가공하여 제조한 식품으로 식품공전에 의하면 빵, 떡류 및 면류 등이 포함된다. 곡류가공식품은 제조과정을 거처 유통 또는 저장하는 동안 제품 자체의 특성과 제품이 노출된 환경의 물리적, 화학적 및 미생물학적 요인에 의하여 품질이 좌우된다. 대표적인 물리적인 요인으로는 온도, 습도, 압력 등이 있고, 화학적인 요인으로는 빛, pH, 산소 등이 있으며, 미생물학적 요인으로는 미생물의 종류와 초기 미생물의 오염농도 등이 있다.Grain-processed foods are processed foods such as bread, rice cake, and noodles. Grain-processed foods are quality-driven by the physical, chemical and microbiological factors of the product itself and the environment in which it is exposed during the process of distribution or storage. Typical physical factors include temperature, humidity, and pressure. Chemical factors include light, pH, and oxygen. Microbiological factors include microbial species and initial microbial contamination levels.

일부 곡류가공식품은 수분을 다량 함유하고 있으므로 특히 유해 미생물의 번식에 의한 변질 가능성이 높고, 장기간 저장할 경우 지방의 산패 등에 의한 관능적 품질의 저하를 가져온다. 이러한 문제를 해결하기 위하여 일부 곡류가공식품에는 합성 방부제 또는 보존제가 사용되고 있다. 그 예로 빵 및 떡류 제품에는 프로피온산 및 그 염, 소르빈산과 그 염 등의 합성 보존료가 사용되고 있고 각종 산도조절제 등도 첨가제로 사용된다. 또한 산도조절제에 포함되어 있는 황산알루미늄칼륨(명반)과 같은 알루미늄 화합물은 인체 내 알루미늄 축적의 원인이 되고 인산염은 미네랄 흡수를 저해할 수 있으므로 장기간 섭취하면 인체에 해로운 것으로 알려져 있다. 면류에는 합성 보존료의 사용이 전면 금지되어 있으므로 칼국수, 생면 등 수분이 다량 함유된 제품은 특히 유해 미생물의 성장에 의한 변질 가능성이 매우 높다. 이에 따라 일부 제품에는 미생물 성장을 억제하기 위하여 주정이 사용되고 있으나, 주정은 제품의 지방산패 방지에는 매우 제한적이며 면의 조리 시 증발 및 세척에 의하여 대부분 제거되므로 제품 자체에 대한 건강기능성의 강화에는 아무런 도움을 주지 못한다. 따라서 보다 효과적으로 곡류가공식품의 보존성을 향상시킬 수 있는 기술의 개발이 요구되고 있다.Some processed cereal grains contain a large amount of water, so there is a high possibility of deterioration due to the propagation of harmful microorganisms, and when stored for a long time, the sensory quality is deteriorated due to rancidity of the fat and the like. To solve these problems, synthetic preservatives or preservatives have been used in some cereal processed foods. For example, synthetic preservatives such as propionic acid and its salts and sorbic acid and its salts are used in bread and rice products, and various acidity regulators are also used as additives. In addition, aluminum compounds such as aluminum potassium sulfate (alum) contained in the acidity regulator cause accumulation of aluminum in the human body and phosphate may inhibit the absorption of minerals. Therefore, long-term ingestion is known to be harmful to human body. Since the use of synthetic preservatives is totally prohibited in the noodles, products containing a large amount of water, such as noodles and noodles, are highly likely to be altered by growth of harmful microorganisms. As a result, some products are used to inhibit microbial growth. However, alcohol is very limited to prevent fatty acid corrosion of products, and most of them are removed by evaporation and washing when cooked. I can not give. Therefore, development of a technology capable of improving the preservability of cereal-processed foods more effectively is required.

이와 관련하여, 대한민국 등록특허 제10-0505786호에 유기산으로 처리한 세척수로 쌀을 세척하고 유기산을 첨가한 침지수로 세척된 쌀을 침지하여 미생물을 제거하고 성장을 억제함으로써 장기보존이 가능한 떡을 제조하는 방법이 개시되어 있다. 그러나 이 방법은 유기산을 다량 사용하고 사용된 유기산이 대부분 폐수로 폐기됨에 따라 원가부담이 높고 오염물질이 다량 함유된 폐수의 방류가 불가피하며, 유기산이 제품에 혼합됨에 따라 제품의 관능성이 저하되는 문제점이 있다. 대한민국 등록특허 제10-0877505호에는 변성전분과 정백당을 사용하여 떡을 제조하고 성형한 떡을 진공 포장하여 보존기간을 향상하는 방법이 개시되어 있는데, 이 기술은 일부 호기성 미생물의 성장을 억제할 수 있지만 포자를 형성하는 혐기성 미생물의 성장억제에는 매우 제한적이며 제품에 대한 지방산패 감소 및 건강기능성 향상 여부는 알 수 없다는 문제가 있다.In this regard, in Korean Patent No. 10-0505786, rice was washed with organic acid-treated washing water, rice was washed with an organic acid-added soaked rice to remove microorganisms, and growth was inhibited. A method for producing the same is disclosed. However, this method has a high cost burden due to the use of a large amount of organic acids and disposal of organic acids used as waste water. Therefore, it is inevitable to discharge wastewater containing a large amount of contaminants. When the organic acid is mixed with the product, There is a problem. Korean Patent Registration No. 10-0877505 discloses a method of improving the preservation period by vacuum-packing rice cake, which is prepared by using modified starch and white sugar, and molding the rice cake. This technology is capable of inhibiting the growth of some aerobic microorganisms However, the inhibition of the growth of anaerobic microorganisms forming spores is very limited, and there is a problem that it is impossible to know whether the decrease in lipid peroxidation and the improvement in health functional properties of the product are unknown.

일반적으로 소나무 수피(bark)란 소나무줄기의 코르크 형성층보다 바깥 조직 즉 검붉은색의 겉껍질 부분을 나타내며, 속껍질은 구황식품으로 알려진 흰색 또는 옅은 갈색 부분으로 이를 송기라고도 한다. 소나무 겉껍질과 속껍질의 성분조성과 특성은 매우 상이한 것으로 알려져 있다. 겉껍질은 주로 리그닌과 폴리페놀 성분으로 구성되어 있고 속껍질은 주로 섬유소 성분으로 구성되어 있다. 소나무 속껍질은 사부(phloem)와 코르크 형성층(cambium)으로 구성되며 이들 조직의 세포분열로 인하여 나무의 비대성장이 이루어지는 부분이므로 겉껍질을 제거한 후에야 얻을 수 있고, 그 수율도 겉껍질에 비하여 매우 낮아서 원료의 대량 확보가 어렵다. 또한 속껍질은 높은 수분 함량으로 인하여 변질의 가능성이 높아서 건조과정이 필요하다.In general, pine bark refers to the outer part of the pine tree stem, ie, the outer part of the pine tree, ie, the reddish brown skin. The inner part of the pine bark is white or light brown, also known as the pungent food. The composition and characteristics of pine crust and mantle are very different. The crust is mainly composed of lignin and polyphenol components, and the inner skin consists mainly of fibrin components. The pine tree trunk is composed of phloem and cambium. It is the part where the growth of the tree occurs due to the cell division of these tissues. Therefore, it can be obtained only after removing the crust, and the yield is also very low as compared with the crust. It is difficult to secure a large amount of In addition, the inner skin has a high possibility of deterioration due to its high moisture content, thus requiring a drying process.

대한민국 공개특허 제10-2012-0060709호에 송기, 솔잎, 물푸레나무 추출액을 이용한 송기떡의 제조방법이 개시되어 있는데, 여기서 송기는 주로 섬유소를 제공하고 솔잎과 물푸레나무추출액은 항균성분을 제공한다. 또한 대한민국 공개특허 제10-1997-0032453호에 송기분말과 송엽을 함유한 면류의 제조방법이 개시되어 있다. 이들 발명에서 송기를 첨가하는 것은 송기의 영양성분을 섭취할 수 있도록 하기 위한 것으로, 이로 인해 보존성이 향상되거나 산패방지 효과가 있는지 알 수 없고, 위에서 설명한 바와 같이 송기를 사용함에 따른 문제점이 있다.Korean Patent Laid-Open Publication No. 10-2012-0060709 discloses a method for producing a rice paddy rice cake using a paddy rice pine, pine needles and an ash rice extract, wherein the rice paddy provides mainly cellulose, and the pine needles and ash tree extract provide an antibacterial component. Korean Patent Laid-Open Publication No. 10-1997-0032453 also discloses a method for producing a noodle containing a powdery feed and a bark. In the present invention, the addition of the air-feeding device is intended to allow the nutrients of the air-feeding device to be consumed. As a result, it is impossible to know whether the preservation property is improved or the effect of preventing rancidity.

소나무는 주로 목재, 펄프원료, 연료 등으로 사용되어 왔고 수피는 부산물로 발생되어 대부분 폐기되고 있으며 일부는 연료, 퇴비, 원예용, 장식용으로 사용되고 있다. 옛날 춘궁기에 식량이 부족할 경우 농촌지역에서는 살아있는 소나무의 속껍질을 벗겨서 식용으로 사용된 적도 있었다. 그러나 소나무 수피를 위생적으로 가공하여 분말로 제조한 후 이를 식품제조 중에 혼합하여 식품의 보존성을 높이고 수피에 함유된 천연 폴리페놀로 인한 건강기능성이 강화된 곡류가공식품은 아직 알려진 바 없다. 특히 수피에 풍부하게 함유된 천연 폴리페놀 성분은 활성산소를 제거하는 항산화 효능뿐만 아니라 혈행 개선, 항염증, 항균, 피부미용 효과, 운동능력 증진, 탈취 효능 등 다양한 생리활성을 지니고 있으므로 곡류가공식품에 혼합하여 가공하게 되면 자연친화적인 웰빙형 기능성 식품을 제공할 수 있는 강점을 지닌다.Pine has mainly been used as wood, pulp raw material, fuel, etc., and the bark is generated as a by-product and is mostly discarded, and some of it is used for fuel, compost, gardening and decorative purposes. In the past, there was a shortage of food in rural areas, where peeled live pine trees were used for food. However, there is no known cereal processed food having enhanced health function due to the natural polyphenol contained in the bark, by enhancing the preservation of the food by mixing the pine bark with hygienically prepared powder and mixing it with the food. Especially, the natural polyphenol ingredient rich in bark has various physiological activities such as antioxidant activity to remove active oxygen, anti-inflammation, anti-inflammation, antibacterial, skin care effect, exercise capacity enhancement and deodorizing effect. It is possible to provide a natural-friendly, well-being type functional food.

본 발명자는 곡류가공식품의 보존성을 향상시키면서 인체에 유익한 성분이 다량 함유되도록 하여 이에 따른 기능성을 부여하고 본연의 관능성을 유지하거나 개선하기 위하여 다양한 연구를 시도하였다. 이의 결과, 곡류가공식품을 제조할 때 소나무 수피 분말을 첨가하면 제조된 곡류가공식품의 미생물 오염도가 줄어들고 지방산패가 방지되며 소나무 수피에 함유된 폴리페놀 등의 다양한 성분으로 인해 인체에 유익한 기능성을 나타낼 수 있음을 확인하고 본 발명을 완성하게 되었다.The present inventors have conducted various studies to improve the preservability of cereal processed foods, to ensure that a large amount of beneficial ingredients are contained in the human body, to thereby impart functionality thereto and to maintain or improve the inherent functionality. As a result, when pine bark powder is added to the processed foods, the microbial contamination of the processed cereal products is reduced, fatty acid loss is prevented, and various components such as polyphenols contained in the pine bark can exhibit beneficial functions to the human body And the present invention was completed.

대한민국 등록특허 제10-0505786호Korean Patent No. 10-0505786 대한민국 등록특허 제10-0877505호Korean Patent No. 10-0877505 대한민국 공개특허 제10-2012-0060709호Korean Patent Publication No. 10-2012-0060709 대한민국 공개특허 제10-1997-0032453호Korean Patent Publication No. 10-1997-0032453

따라서 본 발명의 주된 목적은 보존성이 우수하면서 인체에 유익한 성분이 다량 함유되어 기능성을 나타내며 관능성 또한 우수한 곡류가공식품의 제조방법을 제공하는데 있다.Accordingly, it is a main object of the present invention to provide a method for producing a processed cereal product which is excellent in preservability and contains a large amount of components beneficial to the human body, and exhibits functionality and functionality.

본 발명의 다른 목적은 상기 제조방법으로 제조되어 기능성 및 보존성을 갖는 우수한 품질의 곡류가공식품을 제공하는데 있다.It is another object of the present invention to provide a cereal processed food of excellent quality which is produced by the above production method and has functionality and preservability.

본 발명의 한 양태에 따르면, 본 발명은 물을 제외한 재료 100중량부에 대해 소나무 수피 분말 0.1 내지 20중량부를 첨가하여 가공하는 것을 특징으로 하는 항산화 활성을 갖고 보존성이 개선된 곡류가공식품 제조방법을 제공한다.According to an aspect of the present invention, there is provided a process for preparing a processed food having an improved antioxidant activity and improved preservability, which comprises adding 0.1 to 20 parts by weight of a pine bark powder to 100 parts by weight of a material excluding water, to provide.

본 발명에서 소나무 수피 분말은 소나무에서 수피를 채취하여 통상의 분말화 공정을 통해 제조할 수 있다. 소나무 수피는 소나무줄기의 코르크 형성층보다 바깥 조직 즉 검붉은색의 겉껍질 부분을 의미하는 것으로, 사부와 코르크 형성층으로 구성되는 송기(소나무 속껍질)와 구별된다. 본 발명에서는 이 소나무 수피 분말이 식품에 직접 함유되므로 세척 및 살균과정을 거치는 것이 바람직하다. 또한, 100 ~ 500매시(mesh)로 분말화하여 제조된 식품을 섭취할 때 이물감을 느끼지 않도록 하고 식품 전체에 골고루 분산될 수 있도록 하는 것이 바람직하다.In the present invention, the pine bark powder can be produced through a conventional pulverization process by collecting bark from pine trees. Pine bark refers to the outer part of the pine tree stem, ie, the outer part of the pine tree stem, which is distinguished from the pine bark (pine bark), which consists of bamboo and cork layers. In the present invention, since the pine bark powder is directly contained in the food, it is preferable that the pine bark powder is washed and sterilized. In addition, it is preferable that when the food prepared by powdering at 100 to 500 mesh is consumed, it is not sensed as a foreign body and can be dispersed evenly throughout the food.

본 발명에서 곡류가공식품은 곡류를 주원료로 가공하여 제조한 식품 모두를 의미하며, 빵, 과자, 스프, 만두, 떡, 면, 음료, 술 등과 이와 유사하게 가공한 제품을 포함한다.In the present invention, cereal processed food refers to all foods prepared by processing cereal as a main raw material and includes bread, pastry, soup, dumpling, rice cakes, cotton, beverage, liquor, and the like.

상기 재료는 최종 곡류가공식품에 그대로 또는 가공에 의해 변형된 채로 포함되는 원재료를 의미하는 것으로, 예를 들어 곡류가공식품이 빵인 경우 재료는 밀가루, 소금, 설탕, 식용유, 효모 등이 될 수 있고, 떡인 경우 쌀가루가 될 수 있으며, 면인 경우 밀가루, 소금 등이 될 수 있다.For example, when the cereal processed food is bread, the material may be wheat flour, salt, sugar, edible oil, yeast, and the like. Rice can be rice flour if it is rice, flour and salt if it is rice.

통상의 방법으로 빵을 제조할 수 있으며 빵 제조에 사용되는 밀가루 등 원재료 사용량 100중량부에 대하여 소나무 수피 분말의 사용량은 0.1 ~ 10중량부로 하는 것이 바람직하다. 사용량이 이보다 적으면 수피성분에 의한 보존성 증대, 산패 억제 및 건강기능성 부여 등의 효과가 떨어지게 되며, 사용량이 많으면 반죽의 글루텐 형성이 저해되어 제빵 품질이 저해될 우려가 있고 색상이 짙은 적갈색으로 변하여 관능성이 저하될 우려가 있다.The amount of the pine bark powder to be used is preferably 0.1 to 10 parts by weight based on 100 parts by weight of the raw material such as wheat flour used for bread production. If the amount is less than the above range, the effects such as increase in preservability by the bark components, inhibition of rancidity and health functionalities are deteriorated, and if the amount of the bark is too large, the gluten formation of the dough is inhibited and the baking quality may be deteriorated. There is a fear that the property may deteriorate.

통상의 방법으로 떡을 제조할 수 있으며 떡 제조에 사용되는 쌀 등 원재료 사용량 100중량부에 대하여 소나무 수피 분말의 사용량은 0.1 ~ 20중량부로 하는 것이 바람직하다. 사용량이 이보다 적으면 수피성분에 의한 보존성 증대, 산패 억제 및 건강기능성 부여 등의 효과가 떨어지게 되며, 사용량이 이보다 많으면 떫은맛이 강하게 되어 떡 고유의 풍미가 저하되고 색상이 짙은 적갈색으로 변하여 관능성이 저하될 될 우려가 있다.It is preferable that the amount of the pine bark powder to be used is 0.1 to 20 parts by weight based on 100 parts by weight of the raw material such as rice used for producing rice cakes. If the amount is less than the above range, the effects such as increase in preservability by the bark components, inhibition of rancidity and impartation of health functionalities are deteriorated. If the amount of the bark is lower than the above range, the flavor of rice cake becomes lower and the color becomes dark reddish brown There is a possibility of becoming.

통상의 방법으로 면을 제조할 수 있으며 면 제조에 사용되는 밀가루 등 원재료 사용량 100중량부에 대하여 소나무 수피 분말의 사용량은 0.1 ~ 10중량부로 하는 것이 바람직하다. 사용량이 이보다 적으면 수피성분에 의한 보존성 증대, 산패 억제 및 건강기능성 부여 등의 효과가 떨어지게 되며, 사용량이 이보다 많으면 밀가루 반죽의 글루텐 형성이 저해되어 제면 품질이 저하되고 색상이 짙은 적갈색으로 변하여 관능성이 저하될 우려가 있다.The amount of the pine bark powder to be used is preferably 0.1 to 10 parts by weight based on 100 parts by weight of the raw material such as wheat flour used in the production of cotton. If the amount is less than the above range, the effects such as increase in preservability by the bark components, inhibition of rancidity and the impartation of health functionalities become ineffective. If the amount is larger than this range, gluten formation in the pastry dough is inhibited and the quality of the noodles is deteriorated and the color becomes dark reddish brown. May be deteriorated.

보다 바람직하게는 빵, 떡, 면 제조 시 상기 소나무 수피 분말을 0.5 내지 4중량부로 첨가하는 것이 좋다. 소나무 수피 분말의 첨가량에 따른 각 빵, 떡, 면의 관능성을 비교한 결과, 첨가량 0.5중량부와 4중량부를 기점으로 비교적 큰 차이가 나타났다. 따라서 관능성을 고려하여 0.5 내지 4중량부로 첨가하는 것이 보다 바람직하다.More preferably, the pine bark powder is added in an amount of 0.5 to 4 parts by weight when preparing bread, rice cakes, and cotton. As a result of comparing the functionalities of each bread, rice cakes and cotton according to the addition amount of the pine bark powder, a relatively large difference was observed from 0.5 parts by weight and 4 parts by weight. Therefore, it is more preferable to add 0.5 to 4 parts by weight in consideration of the functionality.

본 발명에 따르면 소나무 수피성분을 첨가함으로써 유해 미생물의 성장 억제, 지방산패도 감소 등으로 인하여 곡류가공식품을 장기간 보존할 수 있고, 천연 폴리페놀 성분으로 인하여 항산화활성 등 건강기능성을 강화하고, 또한 곡류가공식품의 관능성을 향상할 수 있다.According to the present invention, by adding pine bark components, it is possible to preserve processed foods for a long period of time due to the inhibition of growth of harmful microorganisms and decrease in fatty acid pavement, and the natural polyphenol component enhances health functionalities such as antioxidative activity, The functionality of the food can be improved.

또한 소나무 수피성분을 기존 식품공정에 간편하게 도입하여 웰빙형 곡류가공식품을 대량 제조하는 것이 가능하므로 실용화 성공가능성이 매우 높다. 이에 따라 소나무 수피 성분이 함유된 다양한 곡류가공식품을 개발한다면 소나무의 부가가치증대, 임산농가의 소득증대, 식품산업의 발전에도 크게 기여할 수 있다. 특히 소나무 수피의 천연 폴리페놀 성분이 강력한 항산화 활성, 혈행 개선, 항염증, 항균, 피부미용 개선, 지구력 증진 등의 효능을 나타내어 이를 함유한 곡류가공식품은 당뇨, 고혈압, 치매, 동맥경화, 고지혈증 등의 각종 성인병에 대한 예방과 개선에 효과적일 것으로 기대된다. 이를 적극 활용한다면 소비자의 건강 증진에 기여할 수 있고, 차별화된 웰빙형 기능성 식품을 해외로 수출함으로써 국제수지의 개선에도 크게 기여할 수 있을 것이다.In addition, it is possible to mass-produce well-being type cereal processed food by easily introducing the pine bark component into existing food processing, so that the possibility of commercialization is very high. Accordingly, if a variety of cereal processed foods containing pine bark ingredients are developed, it can contribute to increase of value added of pine trees, income increase of forest farmers and development of food industry. In particular, the natural polyphenol component of pine bark exhibits potent antioxidative activity, blood circulation improvement, anti-inflammation, antibacterial, improvement of skin beauty and endurance enhancement, and the cereal processed foods containing the same have diabetes, hypertension, dementia, arteriosclerosis, Is expected to be effective in preventing and improving various adult diseases. By actively using it, it can contribute to the health promotion of consumers, and by exporting differentiated well-being type functional foods to overseas, it can contribute to the improvement of the international balance.

도 1은 일반 국수(좌)와 본 발명에 따른 국수(우, 적송수피분말 2% 첨가)를 비교한 사진이다.
도 2는 일반 쌀떡과 본 발명에 따른 쌀떡을 비교한 사진으로, 좌측에서부터 쌀가루 100%를 사용하여 제조한 일반 쌀떡, 적송수피분말 1% 쌀떡, 적송수피분말 2% 쌀떡, 적송수피분말 3% 쌀떡의 사진이다.
Fig. 1 is a photograph showing the comparison between a normal noodle (left) and a noodle according to the present invention (right, adding 2% of a zodiacal powder).
FIG. 2 is a photograph showing a comparison between a general rice cake and a rice cake according to the present invention. FIG. 2 is a graph comparing the general rice cake with the rice cake according to the present invention. .

이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하기로 한다. 이들 실시예는 단지 본 발명을 예시하기 위한 것이므로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는다.Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

실시예Example 1.  One. 적송수피분말Reed bark powder 제조 Produce

적송수피분말Reed bark powder

강원도 강릉 인근에서 벌채된 50 ~ 100년생 국산 소나무인 적송(Pinus densiflora Sieb & Zucc.)으로부터 수피 10㎏을 채취하여 이끼 등의 이물질을 수작업으로 제거한 후 정제수를 사용하여 수피를 세척하였다. 세척한 수피를 121℃ 스팀을 사용하여 5분간 살균하고, 80℃ 열풍건조기에서 2시간 건조한 후 햄머밀로 1차 조분쇄하고, 핀밀을 사용하여 2차 분쇄하였다. 분쇄 후 100mesh 체를 통과한 적송수피분말 8.7㎏을 수득하였다.10 kg of bark was taken from Pinus densiflora Sieb & Zucc., A 50 ~ 100 year old domestic pine tree, which was cut in Gangneung, Gangwon province, and the bark was washed using purified water after removing foreign matter such as moss by hand. The washed bark was sterilized using steam at 121 ° C for 5 minutes, dried in a hot air drier at 80 ° C for 2 hours, first coarsely pulverized with a hammer mill, and secondarily pulverized using a pin mill. After the pulverization, 8.7 kg of a redeposited bark powder having passed through a 100 mesh sieve was obtained.

실시예 2. 적송수피분말 1% 빵 제조Example 2: Raw bark powder 1% bread preparation

상기 실시예 1에서 제조한 적송수피분말을 약 1%로 첨가하여 빵을 제조하였다.Bread was prepared by adding the reed bark powder prepared in Example 1 at about 1%.

강력분 1.0㎏, 소금 20g, 효모 20g, 적송수피분말 11.5g(물을 제외한 빵원재료 중 약 1%에 해당됨), 설탕 50g, 올리브유 50g, 그리고 물 630g을 계량한 후 반죽기에 넣어 10분간 반죽하였다. 반죽을 30℃로 조절된 항온기에 넣어 부피가 약 2.5배가 될 때까지 1차 발효하고, 약 175g 씩 분할한 후 중간 발효는 20분간, 밀대를 사용하여 가스빼기와 성형을 한 후 세 덩이를 빵틀에 넣고 37℃ 항온기에서 부피가 약 2배가 될 때까지 2차 발효를 실시하였다. 200℃로 예열된 오븐에서 30분간 가열하여 빵을 제조하고 냉각한 후 멸균된 용기에 포장하여 냉장 보관하였다.Weighing about 1.0 kg of high-strength powder, 20 g of salt, 20 g of yeast, 11.5 g of recycled bark powder (about 1% of bread raw materials except water), 50 g of sugar, 50 g of olive oil and 630 g of water were weighed and kneaded for 10 minutes in a kneader. The dough was put into a thermostat controlled at 30 ° C, and the primary fermentation was carried out until the volume became about 2.5 times. After dividing about 175g each, the middle fermentation was performed for 20 minutes by using a plunger and degassing and molding. And the secondary fermentation was carried out at a temperature of 37 ° C until the volume became about twice the volume. The bread was prepared by heating in an oven preheated to 200 ° C for 30 minutes, cooled, packed in a sterilized container and refrigerated.

실시예 3. 적송수피분말 2% 빵 제조Example 3: Raw bark powder 2% bread preparation

적송수피분말의 첨가량을 23g(물을 제외한 빵원재료 중 약 2%에 해당됨)으로 하는 것을 제외하고는 실시예 2와 동일한 방법으로 적송수피분말 2% 빵을 제조하였다.A 2% lozenge bran powder was prepared in the same manner as in Example 2, except that the amount of the redeposited bran powder added was changed to 23 g (corresponding to about 2% of the bread raw materials excluding water).

실시예Example 4.  4. 적송수피분말Reed bark powder 1% 떡 제조 1% rice cake manufacturing

실시예 1에서 제조한 적송수피분말을 약 1%로 첨가하여 떡을 제조하였다.The rice bran powder prepared in Example 1 was added at about 1% to prepare rice cakes.

1.0㎏의 쌀을 정제수를 사용하여 2회 세척한 후 3시간 동안 물에 침지하여 수화시킨 다음 물을 제거하였다. 수화된 쌀을 분쇄기를 사용하여 쌀가루로 제조한 후 증기로 30분간 가열하여 수분함량이 약 50중량%가 되도록 호화시켰다. 호화된 쌀가루와 적송수피분말 10g(물을 제외한 떡원재료 중 약 1%에 해당됨)을 균일하게 혼합한 후 90℃에서 떡기계로 압출 성형하여 가래떡을 제조하였다. 생산된 가래떡을 길이 10cm로 절단한 후 멸균된 용기에 포장하여 냉장 보관하였다.1.0 kg of rice was washed twice with purified water, immersed in water for 3 hours to hydrate, and then water was removed. The hydrated rice was prepared into a rice flour using a pulverizer and then heated with steam for 30 minutes to give a water content of about 50% by weight. 10 g of the granulated rice flour and rice bran powder (about 1% of the raw materials except for water) were uniformly mixed and then extruded at 90 ° C with a rice cake machine to prepare a rice cake. The produced rice cakes were cut into 10 cm lengths, packed in sterilized containers and refrigerated.

실시예Example 5.  5. 적송수피분말Reed bark powder 2% 떡 제조 2% rice cake manufacturing

호화된 쌀가루에 적송수피분말 20g(물을 제외한 떡원재료 중 약 2%에 해당됨)을 혼합하는 것을 제외하고는 실시예 4와 동일한 방법으로 적송수피분말 2% 떡을 제조하였다.A rice bran powder 2% rice cake was prepared in the same manner as in Example 4, except that 20 g of the rice bran powder (corresponding to about 2% of the raw material of rice cake except for water) was added to the grated rice flour.

실시예Example 6.  6. 적송수피분말Reed bark powder 1%  One% 생면cotton wool 제조 Produce

실시예 1에서 제조한 적송수피분말을 약 1%로 첨가하여 생면을 제조하였다.The raw surface was prepared by adding about 1% of the redeposited bark powder prepared in Example 1.

중력분 1.0㎏, 소금 20g, 적송수피분말 10g(물을 제외한 생면원재료 중 약 1%에 해당됨), 그리고 물 500g을 계량한 후 반죽기에 넣어 10분간 반죽한 다음 냉장고에서 10시간 숙성하였다. 숙성된 반죽을 국수 제조기를 사용하여 면대를 형성한 다음 두께 1.5㎜, 폭 5㎜, 길이 210㎜의 생면을 제조하고 멸균된 용기에 포장하여 냉장 보관하였다.Weighing 1.0 g of gravity powder, 20 g of salt, 10 g of powdered redeposited flour (about 1% of raw raw material except water) and 500 g of water were weighed and kneaded in a kneader for 10 minutes and then aged in a refrigerator for 10 hours. The aged dough was formed into a surface by using a noodle maker, and the raw noodles having a thickness of 1.5 mm, a width of 5 mm and a length of 210 mm were prepared, packed in a sterilized container, and refrigerated.

실시예Example 7.  7. 적송수피분말Reed bark powder 2%  2% 생면cotton wool 제조 Produce

생면의 재료에 적송수피분말 20g(물을 제외한 생면원재료 중 약 2%에 해당됨)을 혼합하는 것을 제외하고는 실시예 6과 동일한 방법으로 적송수피분말 2% 생면을 제조하였다.2% raw nodules of the redeposited bark powder were prepared in the same manner as in Example 6, except that 20 g of the redeposited bark powder (corresponding to about 2% of the raw material raw material except for water) was mixed with the noodle material.

비교예 1. 적송수피성분 미첨가 빵 제조COMPARATIVE EXAMPLE 1 Preparation of Bread Without Ingredient

적송수피성분이 함유된 빵과 비교하기 위하여 실시예 2에서 적송수피분말을 첨가하지 않고 적송수피성분 미첨가 빵을 제조하였다.In order to compare with the bread containing the reclaimed bark ingredients, Example 2 was made without adding the reclaimed bark powder to the undiluted bark of the recipe bark.

비교예 2. 적송수피성분 미첨가 떡 제조Comparative Example 2: Preparation of rice cake without rice husk ingredient

적송수피성분이 함유된 떡과 비교하기 위하여 실시예 4에서 적송수피분말을 첨가하지 않고 적송수피성분 미첨가 떡을 제조하였다..In order to compare with the rice cake containing the rice husk ingredient, the rice cake without the rice husk ingredient powder was prepared without adding the rice husk powder in Example 4.

비교예 3. 적송수피성분 미첨가 생면 제조COMPARATIVE EXAMPLE 3 Preparation of Uncooked Raw Ingredients of Recycled Bark Component

적송수피성분이 함유된 생면과 비교하기 위하여 실시예 6에서 적송수피분말을 첨가하지 않고 적송수피성분 미첨가 생면을 제조하였다.In order to compare with the raw noodles containing the raw noodle ingredients, the raw noodles without the raw noodles were prepared without adding the raw noodle powder in Example 6.

실험예 1. 적송수피성분 혼합에 따른 미생물 성장 억제Experimental Example 1. Inhibition of Microbial Growth by Mixing Components of Recycled Bark

적송수피성분 첨가에 따른 저장기간 중 곡물가공식품에 함유된 미생물 성장 억제 효과를 확인하고자 다음과 같이 실험을 수행하였다.In order to investigate the inhibition effect of microbial growth on the processed food of grains during the storage period according to the addition of the reclaimed husk ingredient, the following experiment was carried out.

생면은 높은 수분함량으로 인하여 냉장 유통됨에 따라 냉장온도의 상한선인 10℃에서 12일간 저장한 후 총균수, 대장균군수, 대장균수의 변화를 측정하였고, 빵과 떡은 상온 유통이 가능하므로 25℃에서 3일, 6일, 9일, 12일 저장하면서 곰팡이의 발생유무를 육안으로 관찰하였다. 균수 측정을 위한 전처리로 생면 10g을 90㎖ 0.85% 멸균생리식염수와 혼합하여 1분간 균질기에서 분쇄한 후 시료로 사용하였다. 전처리를 통해 제조한 시료를 9㎖의 0.85% 멸균생리식염수 시험관에 차례로 희석하였고, 희석한 시료 1㎖를 3M Petrifilm에 접종하여 35℃에서 24 ~ 48시간 배양한 후 세균수를 측정하였다. 대장균군수와 대장균수는 동일한 방법으로 접종한 후 각각 35℃에서 48시간 배양하여 균수를 측정하였다.As the moisture content of fresh noodles was increased, the total number of bacteria, E. coli and E. coli were measured after storage for 12 days at the upper limit of the refrigeration temperature as refrigerated circulation. As bread and rice cake could be circulated at room temperature, 3 days, 6 days, 9 days, and 12 days, the occurrence of fungi was visually observed. 10 g of raw noodles were mixed with 90 ml of 0.85% sterilized physiological saline solution for pretreatment for bacterial counting and were ground in a homogenizer for 1 minute and used as a sample. Samples prepared by the pretreatment were sequentially diluted in 9 mL of 0.85% sterile physiological saline, and 1 mL of the diluted samples were inoculated into 3M Petrifilm and incubated at 35 ° C for 24 to 48 hours, and the number of bacteria was measured. Escherichia coli and E. coli were inoculated in the same manner and then cultured at 35 ° C. for 48 hours.

적송수피성분이 첨가된 생면의 저장기간에 따른 총균수, 대장균군수, 대장균수에 대한 결과는 표 1과 같다. 생면을 제조한 후 초기 총균수는 2.1 ~ 3.3×102으로 거의 유사하였으나, 10℃에서 12일간 저장한 후 측정한 균수는 비교예 3(적송수피성분 미첨가)의 생면에서 5.6×106으로 초기 균수에 비하여 약 17,000배 증가하였다. 반면 실시예 6(적송수피분말 1%)의 생면에서 3.9×104으로 약 126배, 실시예 7의 생면(적송수피분말 2%)에서 9.7×103으로 약 29배로 비교예 3의 생면에 비하여 총균수가 크게 감소함에 따라 적송수피분말과 적송수피추출물이 생면의 보존성 향상에 매우 효과적임을 알 수 있다. 한편, 모든 생면 시료에서 대장균군과 대장균은 저장기간 중 검출되지 않았다.Table 1 shows the results for the total number of bacteria, the number of coliform bacteria, and the number of coliform bacteria according to the storage period of the raw noodles to which the extract was added. The initial total number of bacteria after the preparation of noodles was almost the same as 2.1 ~ 3.3 × 10 2 , but the number of bacteria measured after storing for 12 days at 10 ° C was 5.6 × 10 6 in the noodles of Comparative Example 3 Which was about 17,000 times higher than the initial number of bacteria. While exemplary raw noodles of Example 6 (red pine bark powder, 1%) to about 126-fold in a 3.9 × 10 4 raw noodles of Example 7, raw noodles (red pine bark powder 2%), for example in the comparison of 29 to 9.7 × 10 3 times 3 As the total number of bacillus was decreased, it was found that the extracts of Bombyx mori L. and Bombyx mori extract were very effective for improving the preservation of noodles. On the other hand, E. coli and Escherichia coli were not detected during storage in all nude samples.

시료sample 초기 균수(0 일)Initial number of bacteria (0 day) 10℃ 12일 저장 후 균수After storing at 10 ℃ for 12 days, the number of bacteria 총균수Total number of bacteria 대장균군수Coliform 대장균수Coliform number 총균수Total number of bacteria 대장균군수Coliform 대장균수Coliform number 비교예 3Comparative Example 3 3.3×102 3.3 × 10 2 NDND NDND 5.6×106 5.6 × 10 6 NDND NDND 실시예 6Example 6 3.1×102 3.1 x 10 2 NDND NDND 3.9×104 3.9 × 10 4 ND ND NDND 실시예 7Example 7 2.8×102 2.8 × 10 2 NDND NDND 9.7×103 9.7 x 10 3 NDND NDND

ND : Not detectedND: Not detected

적송수피성분이 첨가된 빵과 떡의 저장기간 중 곰팡이의 발생시기 대한 결과는 표 2와 같다. 비교예 1의 빵(적송수피성분 미첨가)은 6일후 곰팡이가 발생하였고, 실시예 2의 빵(적송수피분말 1%)은 9일후, 실시예 3(적송수피분말 2%)의 빵은 12일후에 곰팡이가 발생하였다. 이는 적송수피성분이 빵에 대한 곰팡이의 발생을 지연함에 따라 빵 제품의 저장성 증대에 효과적임을 알 수 있다. 또한 비교예 2의 떡(적송수피성분 미첨가)은 3일후에 곰팡이가 발생하였으나, 실시예 4의 떡(적송수피분말 1%)은 6일후, 실시예 5의 떡(적송수피분말 2%)은 9일후에 곰팡이가 발생하였다. 이는 적송수피성분이 떡에 대한 곰팡이의 발생을 지연함에 따라 떡 제품의 저장성 증대에도 매우 효과적임을 알 수 있다.Table 2 shows the results of the occurrence of fungi during the storage period of the bread and rice cakes to which the dried bark components were added. The bread of Comparative Example 1 (no addition of the reclaimed bark powder) showed mold after 6 days, the bread of Example 2 (1% of the recycled bark powder) after 9 days, the bread of Example 3 Fungi occurred later. This suggests that it is effective to increase the shelf - life of bread products by delaying the occurrence of fungi on the bread. In addition, the rice cake of Comparative Example 2 (the rice husk ingredient not added) had molds after 3 days, but the rice cake (1% of the rice bran powder) of Example 4 had a rice bark (2% of the rice bran powder) of Example 5 after 6 days, The mold developed after 9 days. This suggests that the composition of rice bran is very effective to increase the storage stability of rice cake as the delayed occurrence of mold to rice cake.

시료sample 곰팡이 발생시기 (일)Fungal development period (days) 00 33 66 99 1212 비교예 1Comparative Example 1 XX XX OO OO OO 실시예 2Example 2 XX XX XX OO OO 실시예 3Example 3 XX XX XX XX OO 비교예 2Comparative Example 2 XX OO OO OO OO 실시예 4Example 4 XX XX OO OO OO 실시예 5Example 5 XX XX XX OO OO

X: 곰팡이가 육안으로 관찰되지 않음X: Mold is not visually observed

O: 곰팡이가 육안으로 관찰됨O: Mold was visually observed

실험예 2. 적송수피성분 혼합에 따른 항산화활성Experimental Example 2. Antioxidative activity according to the mixing of the components of the redeposited hulls

항산화활성측정을 위한 시료 준비Sample preparation for antioxidant activity measurement

상기 실시예에서 제조한 곡류가공식품을 동결건조한 후 분쇄하고 10g의 시료를 취하여 100㎖의 메탄올로 50℃에서 1시간 추출한 다음 여과하였다. 여과액의 메탄올을 제거한 후 분말상의 추출물을 제조하여 추출물의 항산화 활성을 측정하였다. 각 추출물 시료를 메탄올에 녹여 각 농도별(50, 100, 300, 500, 700㎍/㎖)로 항산화활성 측정용 시료를 준비하였다.The cereal processed foods prepared in the above examples were lyophilized and pulverized, and 10 g of the sample was taken out and extracted with 100 ml of methanol at 50 ° C for 1 hour and then filtered. After removing the methanol from the filtrate, the powdery extract was prepared and the antioxidative activity of the extract was measured. Samples of each extract were dissolved in methanol and samples for measuring antioxidant activity were prepared at each concentration (50, 100, 300, 500, 700 μg / ml).

항산화활성 측정방법How to measure antioxidant activity

시료의 항산화활성을 조사하기 위해 DPPH(1,1-디페닐-2-피크릴히드라진) 라디칼 소거활성을 측정하였다. 곡물가공식품 건조물 100g에 함유된 항산화활성을 총량 개념을 도입하여 비교 측정하였다. 이때 항산화활성시험의 지표로서 대표적인 항산화제인 BHA 및 비타민 E의 수용성 화합물인 Trolox를 사용하였다.DPPH (1,1-diphenyl-2-picrylhydrazine) radical scavenging activity was measured to examine the antioxidative activity of the sample. The antioxidant activity of 100g of dried food of cereal processed food was compared with the total amount concept. BHA, a typical antioxidant, and Trolox, a water-soluble compound of vitamin E, were used as indicators of antioxidant activity.

DPPH 라디칼 소거활성 측정법Measurement of DPPH radical scavenging activity

96-well 플래이트의 각 well당 각 농도별 시료용액 10㎕를 가하고, 에탄올에 녹인 1.5×10-4M DPPH 용액 90㎕를 첨가한 후 10분간 반응시키고 마이크로플래이트 측정기로 517nm에서 흡광도를 측정하였다. 농도에 따른 DPPH 라디칼 소거활성을 다음 식에 의거하여 계산하였다. DPPH 용액 대신 에탄올을 첨가한 시료의 흡광도를 blank로 하였으며, 시료 대신 메탄올을 넣은 것을 대조구로 하였다.10 μl of sample solution for each concentration was added to each well of a 96-well plate, and 90 μl of a 1.5 × 10 -4 M DPPH solution dissolved in ethanol was added. The reaction was allowed to proceed for 10 minutes and absorbance was measured at 517 nm using a microplate meter. The concentration of DPPH radical scavenging activity was calculated according to the following equation. The absorbance of the sample to which ethanol was added instead of the DPPH solution was blank, and methanol was added instead of the sample to give a control.

PPH 라디칼 소거활성(%) = (10분 후 측정한 시료의 흡광도 변화) × 100/대조구의 흡광도PPH radical scavenging activity (%) = (absorbance change of sample after 10 minutes) × 100 / absorbance of control

상기 DPPH 라디칼 소거활성에서 얻은 각 시료의 흡광도 결과로부터 라디칼 형성을 50% 억제하는데 필요한 시료의 농도를 ED50(㎍/㎖)값으로 나타내어 각 시료의 항산화활성을 비교하였으며, 각 시료의 항산화활성을 총량개념으로 환산한 총량 항산화활성(추출수율×상대적 항산화활성)을 비교하였다. 시료의 ED50 값이 낮을수록 시료는 높은 항산화활성을 나타낸다. 표 3에서와 같이 각 시료의 항산화물질에 대한 추출수율은 0.98 ~ 1.45%를 나타내었고 적송수피분말이 함유된 시료에서 높은 추출수율을 보였다. 또한 추출물에 대한 DPPH 소거활성은 적송수피분말이 함유된 시료에서 높은 활성을 나타내었고, 총량 개념으로 환산한 항산화활성도 유사한 결과를 보였다. 총량 개념으로 환산한 항산화활성을 비교하면 다음과 같다. 빵의 경우 비교예 1의 빵(적송수피성분 미첨가)과 비교한 항산화활성은 실시예 2의 빵(적송수피분말 1%)이 약 1.7배, 실시예 3의 빵(적송수피분말 2%)이 약 2.6배 증가하였다. 떡의 경우 비교예 2의 떡(적송수피성분 미첨가)에 비교한 항산화활성은 실시예 4의 떡(적송수피분말 1%)이 약 1.9배, 실시예 5의 떡(적송수피분말 2%)이 약 2.9배 증가하였다. 생면의 경우 비교예 3의 생면(적송수피성분 미첨가)에 비교한 항산화활성은 실시예 6의 생면(적송수피분말 1%)이 약 1.9배, 실시예 7의 생면(적송수피분말 2%)이 약 3.2배 증가하였다. 이에 따라 적송수피성분이 곡물가공식품의 항산화활성 증대에 매우 효과적임을 알 수 있다.The antioxidant activity of each sample was compared with the ED50 (㎍ / ㎖) value of the sample required to inhibit radical formation by 50% from the absorbance of each sample obtained from the DPPH radical scavenging activity. (Total yield) × relative antioxidant activity (total antioxidant activity). The lower the ED50 value of the sample, the higher the antioxidative activity of the sample. As shown in Table 3, the extraction yield of each of the antioxidants was 0.98-1.45%, and the extraction yield of the extract containing the redeposited bark powder was high. The DPPH scavenging activity of the extracts showed high activity in the samples containing the redeposited bark powder, and the antioxidant activity in terms of the total amount concept showed similar results. The total antioxidant activity was converted into the following concept. The antioxidative activity of the bread (Comparative Example 1) (about 1% of the recycled bark powder) was about 1.7 times, the bread of Example 3 (the reclaimed bark powder of 2%), Which was about 2.6 times higher than that of the control group. In case of rice cakes The antioxidative activity of the rice cakes (1% of the recycled bark powder) and the rice cake (2% of the recycled bark powder) of Example 4 were about 1.9 times, Which was about 2.9 times higher than that of the control group. The antioxidative activity of the raw noodles of Example 6 (1% of the recycling bark powder) was about 1.9 times, the noodles of Example 7 (2% of the recycling bark powder) Which was about 3.2 times higher than that of the control group. Therefore, it can be understood that the dried bark component is very effective in increasing the antioxidant activity of the processed food of cereals.

시료sample 추출수율(%)Extraction yield (%) DPPH 소거 활성
(ED50,㎍/㎖)
DPPH scavenging activity
(ED50, 占 퐂 / ml)
추출물의 상대적 항산화활성도(%)(Trolox 대비)Relative antioxidant activity of extract (%) (compared to Trolox) 항산화활성 (총량 개념)Antioxidant activity (total amount concept)
BHABHA -- 6.5 6.5 392.0 392.0 -- TroloxTrolox -- 25.5 25.5 100.0 100.0 -- 비교예 1Comparative Example 1 1.191.19 121.4 121.4 21.0 21.0 25.0 25.0 실시예 2Example 2 1.311.31 79.7 79.7 32.0 32.0 41.9 41.9 실시예 3Example 3 1.421.42 56.7 56.7 45.0 45.0 63.9 63.9 비교예 2Comparative Example 2 0.820.82 141.7 141.7 18.0 18.0 14.8 14.8 실시예 4Example 4 0.920.92 85.0 85.0 30.0 30.0 27.6 27.6 실시예 5Example 5 1.031.03 62.2 62.2 41.0 41.0 42.2 42.2 비교예 3Comparative Example 3 0.980.98 134.2 134.2 19.0 19.0 18.6 18.6 실시예 6Example 6 1.091.09 77.3 77.3 33.0 33.0 36.0 36.0 실시예 7Example 7 1.171.17 50.0 50.0 51.0 51.0 59.7 59.7

실험예 3. 적송수피성분 혼합에 따른 지방산패도(TBARS, thiobarbituric acid reactive substance)Experimental Example 3: Preparation of a fatty acid group (TBARS, thiobarbituric acid reactive substance)

동결건조한 후 분쇄한 곡물가공식품 시료 10g을 oxygen-permeable polyethylene zip-lock bag에서 넣고 30℃ 항온실에서 0, 10, 20, 30일간 저장한 후 지방산패도(TBARS)를 측정하였다. 지방산패도 측정을 위하여 시료 0.5g과 증류수 15㎖을 혼합하여 Homogenizer(Tissue grinder, 1102-1, Japan) 로 13,500rpm에서 5분간 균질하였다. 균질액 1㎖과 Butylated hydroxyanisole 50㎕, 60℃에서 용해한 thiobarbituric acid 1.3%를 함유하는 50%의 trichloroacetic acid 혼합용액(TBA/TCA) 2㎖를 가하여 혼합하였다. 발색을 위하여 혼합물을 60℃ 수조에서 1시간 동안 가온한 다음 실온까지 냉각시켜서 3,000rpm에서 15분간 원심분리 후 상등액을 얻었다. Spectrophotometer(Shimadzu UV mini-1240, Japan)를 사용하여 532nm에서 상등액의 흡광도를 측정한 다음, 증류수 1㎖과 TBA/TCA 혼합용액 2㎖을 함유하는 맹검(Blank)의 측정치와 비교하였고, 그 차이 값에 상용계수 5.88을 곱해서 TBARS 량을 MDA(malondiadehyde) ㎎/㎏으로 표시하였다. MDA 형성을 위하여 수용액에서 스스로 분해되는 Tetrathoxypropane(Sigma, USA)를 표준물질로 사용하였다.After 10, 20, and 30 days storage at 30 ° C in an oxygen-permeable polyethylene zip-lock bag, 10 g of the sample was added to freeze-dried, ground soybeans (TBARS). 0.5 g of sample and 15 ml of distilled water were mixed and homogenized at 13,500 rpm for 5 minutes using a homogenizer (Tissue grinder, 1102-1, Japan). 50 ml of a 50% trichloroacetic acid mixed solution (TBA / TCA) containing 1.3 ml of thiobarbituric acid dissolved at 60 ° C was added to 1 ml of the homogenate, 50 μl of butylated hydroxyanisole, and the mixture. For color development, the mixture was heated in a 60 ° C water bath for 1 hour, cooled to room temperature, and centrifuged at 3,000 rpm for 15 minutes to obtain a supernatant. The absorbance of the supernatant was measured at 532 nm using a spectrophotometer (Shimadzu UV mini-1240, Japan), and then compared to blank measurements containing 1 ml of distilled water and 2 ml of TBA / TCA mixed solution, And a commercial factor of 5.88, and the amount of TBARS was expressed as MDA (malondiadehyde) mg / kg. For MDA formation, Tetrathoxypropane (Sigma, USA), which is decomposed by itself in aqueous solution, was used as a standard material.

표 4에서와 같이 각 시료의 지방산패도는 저장기간에 따라 증가하였으나, 적송수피분말이 함유된 시료는 초기의 지방산패도가 크게 감소하였을 뿐만 아니라 저장기간에 따른 지방산패도의 증가도 감소하였다. 빵의 경우, 초기(0일)의 지방산패도는 비교예 1의 빵(적송수피성분 미첨가)에 비하여 실시예 2의 빵(적송수피분말 1%)이 약 0.82배, 실시예 3의 빵(적송수피분말 2%)이 약 0.73배로 적송수피성분이 빵 제조과정 중에도 지방의 산패방지에 효과적임을 알 수 있다. 또한 빵의 저장기간에 따른 지방산패도의 변화는 비교예 1의 빵은 2.0배, 실시예 2의 빵은 약 1.8배, 실시예 3의 빵은 약 1.6배로 적송수피성분이 빵의 저장기간에 따른 지방산패의 억제에도 매우 효과적임을 알 수 있다. 떡의 경우, 초기(0일)의 지방산패도는 비교예 2의 떡(적송수피성분 미첨가), 실시예 4의 떡(적송수피분말 1%), 실시예 5의 떡(적송수피분말 2%)에서 모두 동일하였다. 또한 떡의 저장기간에 따른 지방산패도의 변화는 비교예 2의 떡은 2.2배, 실시예 4의 떡은 약 1.4배, 실시예 5의 떡은 약 1.3배로 적송수피성분이 떡의 저장기간에 따른 지방산패의 억제에 매우 효과적임을 알 수 있다. 또한 생면의 경우, 초기(0일)의 지방산패도는 비교예 3의 생면(적송수피성분 미첨가), 실시예 6의 생면(적송수피분말 1%), 실시예 7의 생면(적송수피분말 2%)에서 모두 동일하였다. 또한 떡의 저장기간에 따른 지방산패도의 변화는 비교예 3의 생면은 2.3배, 실시예 6의 생면은 약 1.6배, 실시예 7의 생면은 약 1.4배로 적송수피성분이 생면의 저장기간에 따른 지방산패의 억제에 효과적임을 알 수 있다.As shown in Table 4, the fatty acid content of each sample increased with storage period. However, in the samples containing the reclaimed rice bran powder, not only the initial fatty acid content was greatly reduced but also the increase in fatty acid content during storage period was also decreased. In the case of bread, the initial (0 day) fatty acid composition was about 0.82 times the bread of Example 2 (1% recollection bark powder) compared to the bread of Comparative Example 1 (the rice bran component was not added) (2%) were about 0.73 times higher than those of the other treatments. In addition, the change in fatty acid composition according to the storage period of the bread was 2.0 times that of the bread of Comparative Example 1, 1.8 times that of the bread of Example 2, and 1.6 times that of the bread of Example 3, It is also very effective in inhibiting local rancidity. In the case of the rice cake, the initial (0 day) fatty acid composition was the same as that of Comparative Example 2 (rice husk ingredient not added), rice cake of Example 4 (rice husk powder 1%), rice cake of Example 5 (rice husk powder 2% ). In addition, the change of fatty acid composition according to the storage period of rice cake was 2.2 times of rice cake of Comparative Example 2, about 1.4 times of rice cake of Example 4, and about 1.3 times of rice cake of Example 5, And it is very effective in inhibiting local rancidity. In the case of the noodles, the initial (0 day) fatty acid composition was the same as that of the noodles of Comparative Example 3 (no addition of the recipe bark components), the noodles of Example 6 (1% of the recipe bark powder) %). The change in fatty acid composition according to the storage period of the rice cake was 2.3 times in the noodles of Comparative Example 3, 1.6 times in the noodles of Example 6, and about 1.4 times in the noodles of Example 7, And it is effective in inhibiting local rancidity.

시료sample 저장기간 (30 ℃)Storage period (30 ℃) 0 일0 days 10 일10 days 20 일20 days 30 일30 days 비교예 1Comparative Example 1 0.22 0.22 0.30 0.30 0.37 0.37 0.44 0.44 실시예 2Example 2 0.18 0.18 0.23 0.23 0.28 0.28 0.32 0.32 실시예 3Example 3 0.16 0.16 0.19 0.19 0.22 0.22 0.25 0.25 비교예 2Comparative Example 2 0.12 0.12 0.16 0.16 0.21 0.21 0.26 0.26 실시예 4Example 4 0.12 0.12 0.14 0.14 0.15 0.15 0.17 0.17 실시예 5Example 5 0.12 0.12 0.14 0.14 0.14 0.14 0.16 0.16 비교예 3Comparative Example 3 0.14 0.14 0.23 0.23 0.28 0.28 0.32 0.32 실시예 6Example 6 0.14 0.14 0.16 0.16 0.19 0.19 0.23 0.23 실시예 7Example 7 0.14 0.14 0.16 0.16 0.17 0.17 0.19 0.19

실험예 4. 적송수피 성분 혼합에 따른 관능검사Experimental Example 4. Sensory Evaluation According to Mixture of Recycled Bark Components

상기 실시예 및 비교예의 빵, 떡, 생면을 4℃ 냉장고에 3일간 저장한 후 각 제품의 관능검사를 시행하였고 그 결과는 표 5와 같다. 관능검사를 위하여 20 ~ 55세로 구성된 관능검사원 24명을 선발하여 맛, 향, 색상, 쫄깃함에 대한 평가를 실시하였고 각각의 최고점은 10점, 최하점은 0점으로 평가하였으며 각 항목의 합계를 더한 후 4로 나누어 종합점수를 산출하였다. 빵의 경우 색에 있어서 적송수피성분 미첨가 빵에 비하여 다소 낮은 점수를 나타냈으나, 다른 항목은 모두 적송수피성분이 첨가된 빵이 높은 점수를 나타내어 종합점수는 높은 결과를 보였다. 떡의 경우에는 적송수피성분 미첨가 떡이 백색이므로 옅은 적갈색을 나타내는 적송수피성분이 첨가된 떡의 색이 높은 점수를 나타내었고 다른 항목의 경우에도 대부분 적송수피성분이 함유된 떡이 높은 관능성을 나타내었다. 생면의 경우에도 적송수피성분의 첨가로 모든 관능검사 항목에 있어서 높은 점수를 나타냄에 따라 적송수피성분의 첨가가 곡류가공식품의 관능성 향상에도 매우 효과적인 방법임을 알 수 있다.The bread, rice cakes, and noodles of the above examples and comparative examples were stored in a refrigerator at 4 ° C for 3 days, and the sensory evaluation of each product was carried out. For sensory evaluation, twenty - four sensory testers, 20 ~ 55 years old, were selected and their taste, aroma, color and chewiness were evaluated. The highest and lowest points were evaluated as 10 points and 0 point respectively. 4, and the total score was calculated. In the case of bread, the score was slightly lower than that of unhulled bread in color, but all the other items showed high scores on bread with recycled bark. In the case of rice cakes, rice cakes with a reddish - brown color showed a high score because rice cakes without rice cakes were white, and rice cakes with a reddish - brown color showed high scores. Respectively. In addition, it was shown that the addition of red pepper bark is a very effective method to improve the sensory properties of cereal processed food.

시료sample 관능검사Sensory test color flavor 향기Scent 쫄깃함Kosi 종합점수Overall score 비교예 1Comparative Example 1 7.00 7.00 7.80 7.80 6.70 6.70 8.50 8.50 7.50 7.50 실시예 2Example 2 6.70 6.70 8.20 8.20 7.50 7.50 8.80 8.80 7.80 7.80 실시예 3Example 3 6.40 6.40 8.50 8.50 7.20 7.20 9.00 9.00 7.78 7.78 비교예 2Comparative Example 2 7.20 7.20 8.20 8.20 7.50 7.50 7.80 7.80 7.68 7.68 실시예 4Example 4 7.90 7.90 8.80 8.80 7.90 7.90 8.00 8.00 8.15 8.15 실시예 5Example 5 8.30 8.30 8.60 8.60 8.10 8.10 7.70 7.70 8.18 8.18 비교예 3Comparative Example 3 7.70 7.70 7.80 7.80 7.20 7.20 6.90 6.90 7.40 7.40 실시예 6Example 6 8.20 8.20 8.30 8.30 7.50 7.50 7.20 7.20 7.80 7.80 실시예 7Example 7 8.30 8.30 8.20 8.20 7.80 7.80 6.80 6.80 7.78 7.78

Claims (4)

물을 제외한 재료 100중량부에 대해 소나무 수피 분말 0.1 내지 20중량부를 첨가하여 가공하는 것을 특징으로 하는 항산화 활성을 갖고 보존성이 개선된 곡류가공식품 제조방법.And 0.1 to 20 parts by weight of a pine bark powder is added to 100 parts by weight of the material excluding water to prepare an antioxidant activity and improved preservability. 제 1항에 있어서,
상기 소나무 수피 분말을 0.1 내지 10중량부로 첨가하는 것을 특징으로 하는 곡류가공식품 제조방법.
The method according to claim 1,
Wherein the pine bark powder is added in an amount of 0.1 to 10 parts by weight.
제 1항에 있어서,
상기 소나무 수피 분말을 0.5 내지 4중량부로 첨가하는 것을 특징으로 하는 곡류가공식품 제조방법.
The method according to claim 1,
Wherein the pine bark powder is added in an amount of 0.5 to 4 parts by weight.
제 1항 내지 제 3항 중 어느 한 항에 있어서,
상기 곡류가공식품은 빵, 떡 또는 면인 것을 특징으로 하는 곡류가공식품 제조방법.
4. The method according to any one of claims 1 to 3,
Wherein the cereal processed food is a bread, a rice cake or a cotton.
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