KR20160023238A - Composition for preventing, improving or treating inflammatory disease and influenza virus infection comprising unripe pepper extract as an active ingredient - Google Patents

Abstract

The present invention relates to anti-inflammatory and anti-influenza virus effects of a green pepper extract and, more specifically, to a pharmaceutical composition and a health functional food composition for preventing and treating inflammasome-mediated inflammatory diseases and influenza virus infection, comprising a green pepper extract as an active ingredient. The present invention has excellent effects of preventing, alleviating, and treating inflammasome-mediated inflammatory diseases and influenza virus infection using the anti-inflammatory and anti-influenza virus effects of the green pepper extract.

Description

TECHNICAL FIELD The present invention relates to a composition for preventing and / or improving influenza-mediated inflammatory diseases and influenza virus infection,

The present invention relates to antiinflammatory and anti-influenza virus effects of blueberry red pepper extract, and more particularly to a composition for preventing, improving and treating inflammatory-mediated inflammatory diseases or influenza virus infection, The present invention also relates to a health functional food characterized by comprising

The inflammatory response is stimulated by foreign substances such as damage, bacteria, fungi and viruses, and a series of complex physiological phenomena such as activation of enzymes by various inflammatory mediators and immune cells, secretion of inflammatory mediators, fluid infiltration, cell migration, Reaction, which leads to symptoms such as erythema, edema, fever, and pain.

Inflammation reaction removes external infectious agents and regenerates damaged tissues to restore the function of living organisms. However, if the inflammation reaction occurs excessively or continuously such as the antigen is not removed or the internal substance is caused, the mucous membrane damage, tissue destruction And cancer, inflammatory skin disease, arthritis, and others.

IL-1β is a cytokine that is induced by pathogen infection and is involved in host defense by regulating immune and inflammatory responses. IL-1β is first secreted into the inactive form (proIL-1β) by induction of expression at the transcription level by stimulation with a pathogen-derived substance, and then secreted out of the cell when it is converted into an active form through aging of protein degradation By strictly segregating and modulating the process, excessive secretion of IL-1β is controlled.

Inflammase is involved in the aging of proIL-1β by activating caspase-1 as a complex protein substance present in the cytoplasm, and there are NLRP1, NLRP3, NLRC4, and AIM2 inflammases as representatives. Because misregulated inflammation activity can lead to severe inflammatory diseases, the relationship between the action of inflammase and congenital and acquired inflammatory diseases has been highlighted in the regulation of immune response.

To date, antihistamines, vitamin ointments and corticosteroids have been mainly used for the treatment of inflammatory diseases. However, the development of new drugs that have a therapeutic effect on inflammatory diseases due to the fact that most of these drugs are temporary, Is required.

Influenza is a virus belonging to the genus Orthomaxoviridae (influenza virus) that enters the mucous membranes and causes respiratory diseases. Viral particles have a coating, spherical shape with a diameter of 80 to 120 nm, but they have polymorphism such as filamentous particle. The viral particles have surface antigens defined by V antigens, namely 16 types of red blood cell aggregates (HA) and 9 kinds of neuramidases (NA), and S antigens (antigens mainly defined as ribonucleoprotein NPs) It is divided into three types of A, B and C by serological specificity.

A type is susceptible to the emergence of new V antigen (HA or NA) mutants by mutation (drift) or dielectrophoretic shift (shift), and when the antibody level to the mutant antigen is low in the human group, it causes pandemic worldwide. In type B, even when a new mutant appears, the mutation of the antigen is continuous, and the epidemic is mainly local. Type C, which proliferates into kidney and fetal cultured cells such as monkeys and cows, has one long genome and the least change in surface antigen.

The antiviral agents developed so far have severe side effects and require a great deal of attention to their application. In addition, development of a vaccine has a problem in that the effectiveness is low unless the viral type of vaccine and vaccine virus are matched. Therefore, there is a growing need for the development of new influenza virus agents with excellent antiviral inhibition and excellent stability.

Accordingly, the inventors of the present invention have been studying traditional traditional food extracts having excellent anti-inflammatory and anti-influenza virus activity, and found that the extract of Cheongpongchu has excellent anti-inflammatory and anti-influenza virus activity and can prevent and prevent infection of inflammatory mediated inflammatory diseases and influenza virus The present invention has been completed.

Accordingly, an object of the present invention is to provide a pharmaceutical composition for the prevention and treatment of inflammatory diseases mediated by inflammation which comprises an extract of Cheongpong (red pepper) as an active ingredient.

It is still another object of the present invention to provide a health functional food composition for preventing and improving inflammatory diseases mediated by inflammation, which comprises an extract of Cheongpong (red pepper) as an active ingredient.

Yet another object of the present invention is to provide a health functional food characterized by comprising a health functional food composition for preventing and improving inflammatory disease mediated inflammatory disease, comprising an extract of Cheong Chu Pung as an active ingredient.

It is still another object of the present invention to provide a pharmaceutical composition for preventing and treating influenza virus infection comprising an extract of Cheongpong (red pepper) as an active ingredient.

It is still another object of the present invention to provide a health functional food composition for preventing and improving influenza virus infection comprising an extract of Cheongpong (red pepper) as an active ingredient.

Yet another object of the present invention is to provide a health functional food characterized by comprising a health potential food composition for preventing and improving influenza virus infection comprising an extract of green pepper as an active ingredient.

In order to accomplish the above object, the present invention provides a pharmaceutical composition for preventing and treating inflammatory disease mediated by inflammoma comprising an extract of Cheongpong (red pepper) as an active ingredient.

In order to accomplish another object of the present invention, the present invention provides a health functional food composition for prevention and improvement of inflammatory diseases mediated by inflammomas comprising an extract of Cheongpong (red pepper) as an active ingredient.

In order to accomplish another object of the present invention, the present invention provides a health functional food comprising a health functional food composition for preventing and improving inflammatory diseases mediated by inflammomas, which comprises an extract of Cheong Chu Pung as an active ingredient.

In order to accomplish another object of the present invention, the present invention provides a pharmaceutical composition for preventing and treating influenza virus infection comprising an extract of Cheongpong (red pepper) as an active ingredient.

In order to accomplish another object of the present invention, the present invention provides a health functional food composition for prevention and improvement of influenza virus infection comprising an extract of Cheongpongchu as an active ingredient.

In order to accomplish still another object of the present invention, the present invention provides a health functional food comprising a health functional food composition for preventing and improving influenza virus infection comprising an extract of green pepper as an active ingredient.

Hereinafter, the present invention will be described in detail.

The pharmaceutical composition of the present invention is effective for the prevention and treatment of inflammatory-mediated inflammatory diseases and influenza virus infections by containing a blueberry extract as an active ingredient.

Blue pepper is a completely mature pepper, and when it is ripe, it becomes red pepper. In Korea, it is cultivated all year round, and usually after the flowers are over, the green pepper can be harvested. In the past, pepper was mainly used as dried red pepper, but the demand for fresh green pepper used for reproductive use was greatly increased along with the trend of consumption of fresh vegetables. Good green pepper is uniform in size and shape, bigger, bigger, cleaner and more tender. The skin is dark green, smooth, thick and light.

The blue pepper extract of the present invention can be extracted by a known natural substance extraction method and is preferably extracted with one or more solvents selected from the group consisting of water, organic solvents having 1 to 6 carbon atoms, and subcritical or supercritical fluids The organic solvent having 1 to 6 carbon atoms may be an alcohol having 1 to 6 carbon atoms, acetone, ether, benzene, chloroform, ethylacetate, methylene chloride and may be selected from the group consisting of methylene chloride, hexane, cyclohexane, and petroleum ether.

The extraction temperature of the extract of the present invention is not particularly limited and may be, for example, from 0 ° C to 90 ° C, preferably from room temperature to 50 ° C. The extraction time of the extract of the present invention is not particularly limited and may be, for example, 1 hour to 10 days, preferably 10 hours to 24 hours.

The extract of the present invention can be extracted by a known natural substance extraction method. For example, it can be extracted by cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction or heat extraction, preferably by hot water extraction or reflux cooling extraction.

The extract of the present invention can be used as a liquid phase by filtration, and can be solidified through a drying process such as spray drying or lyophilization. More preferably, it is possible to mix and dry the texturin before spray drying or lyophilization.

The inflammation-mediated inflammatory diseases of the present invention may be used for the treatment of familial Mediterranean fever, familial cold autoinflalmnatory syndrome, Muckle-Wells syndrome, (PAPA) syndrome, pyoderma gangrenosum, and acne, hyperimmunoglobulin d syndrome, and cryopyrin-associated periodic syndromes. ≪ / RTI >

In one embodiment of the present invention, the anti-inflammatory effect of the blueberry extract was tested in order to inhibit inflammatory activity, an inflammation-regulating complex. As a result, the blue pepper extract of the present invention effectively inhibited ATP-induced NLRP3 inflammase at a concentration of 6.25 mg / mL or higher and NG-induced NLRP3 inflammase at a concentration of 3.125 mg / mL or higher, / mL < / RTI > (see FIG. 1).

Inflammase is present in the cytoplasm of myeloid cells and is a protein complex composed of innate immune receptors (NOD-like receptors), epithelial proteins (ASCs) and caspase-1, 1β (pro-IL-1β), a precursor of inflammatory cytokines associated with innate immune defenses, into the active form IL-1β (p17). There are inflammoses composed of NLRP1, NLRP3, NLRC4, AIM4, etc. Inflammome-mediated processes are important for bacterial infections and are important for both metabolic processes and regulation of mucosal immune responses. For example, mutations in the NLRP3 gene have been shown to cause autoimmune diseases that correspond to cryopyrin-associated periodic syndromes. It has also been found that increased IL-1β is associated with human congenital or acquired diseases, and that antagonists or receptors of IL-1β are helpful in the successful treatment of some diseases. Therefore, the relationship between inappropriate in fl ammers action and congenital and acquired inflammatory diseases has been highlighted in the regulation of immune response. The Puerariae Radix extract of the present invention can effectively control the secretion amount of the inflammatory cytokine IL-1 beta by inhibiting the activity of this inflammase, and thus has an excellent effect for the prevention and treatment of inflammatory diseases.

Influenza is a virus belonging to the genus orthomyxoviridae and the influenza virus, causing respiratory diseases. Influenza virus particles have a coating and spherical shape with a diameter of 80 to 120 nm, but they have polymorphism such as filamentous particle. The viral particles have surface antigens defined by V antigens, namely 13 kinds of red blood cell aggregates (HA) and 9 kinds of neuramidases (NA), and S antigens (antigens mainly defined as ribonucleoprotein NPs) It is divided into three types of A, B and C by serological specificity. A type is susceptible to the emergence of new V antigen (HA or NA) mutants by mutation (drift) or dielectrophoretic shift (shift), and when the antibody ratio to the mutant antigen is low in the human group, Apart from pigs, horses, and ducks, they also proliferate within the organism and cause segmental exchange. In type B, even when a new mutant appears, the mutation of the antigen is continuous, and the epidemic is mainly local. Type C, which proliferates into kidney and fetal cultured cells such as monkeys and cows, has one long genome and the least change in surface antigen.

The influenza virus of the present invention may be preferably an influenza A virus, more preferably a HIN1 virus which is one of the subtypes of influenza A virus.

The disease caused by the influenza virus infection of the present invention may be selected from the group consisting of influenza virus infection such as flu, cold, sore throat, bronchitis, pneumonia, fever, chills, headache and cough, sore throat, runny nose, shortness of breath, But can be preferably flu, cold, sore throat, bronchitis and pneumonia.

In one embodiment of the present invention, the inhibitory effect of influenza virus on the concentration of blue pepper extract was tested together with cytotoxicity. As a result, the cellulolytic activity was low at a concentration of 0.005 mg / mL or less, and the effect of anti-influenza virus was excellent at a concentration of 0.0412 mg / mL (see FIG. 1).

Therefore, the pharmaceutical composition of the present invention comprising the extract of Cheongguk (red pepper) as an active ingredient is effective for the prevention and treatment of inflammatory diseases caused by inflammatory mediated inflammation, and is also effective for prevention and treatment of diseases caused by influenza virus infection.

The pharmaceutical compositions according to the present invention may further comprise a pharmaceutically effective amount of a green pepper extract alone or in combination with one or more pharmaceutically acceptable carriers, excipients or diluents. The term "pharmaceutically acceptable" as used herein means a non-toxic composition which is physiologically acceptable and which, when administered to humans, does not inhibit the action of the active ingredient and does not normally cause an allergic reaction such as gastrointestinal disorder, dizziness, .

Examples of the carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methylcellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. The pharmaceutical composition may further include a filler, an anti-coagulant, a lubricant, a wetting agent, a flavoring agent, an emulsifier, and an antiseptic agent.

As used herein, the term "pharmaceutically effective amount" refers to an amount that exhibits a higher level of response than the negative control, and preferably refers to an amount of the compound of the present invention that is effective for preventing and treating diseases or conditions caused by inflammatory diseases and influenza virus infection Quot; refers to an amount sufficient to exhibit an effect. The pharmaceutically effective amount of the blueberry red pepper extract according to the present invention is 0.01 to 100 mg / day / kg body weight. However, the pharmaceutically effective amount may be appropriately changed depending on various factors such as the type and severity of the disease, the age, body weight, health condition, sex, administration route and treatment period of the patient.

In addition, the pharmaceutical composition of the present invention may be formulated using methods known in the art so as to provide rapid, sustained or delayed release of the active ingredient after administration to the mammal. The formulations may be in the form of powders, granules, tablets, droplets, syrups, aerosols, soft or hard gelatine capsules, sterile injectable solutions, sterile powders.

The administration route of the composition of the present invention is not limited thereto, but it can be administered orally or parenterally. Parenteral routes of administration include, for example, various routes such as transdermal, nasal, peritoneal, muscular, subcutaneous or intravenous.

The pharmaceutical composition of the present invention can be administered in combination with a known compound having the effect of preventing and treating diseases caused by inflammatory-mediated inflammatory diseases and influenza virus infection.

The present invention also provides a health functional food composition for preventing and ameliorating inflammatory diseases mediated by inflammatory mediators comprising an extract of Cheongpong (red pepper) as an active ingredient, and a health functional food comprising the same.

The health functional food composition of the present invention is effective for prevention and improvement of inflammatory diseases mediated by inflammation, including blue pepper extract as an active ingredient.

Also, the present invention provides a health functional food composition for preventing and improving influenza virus infection comprising an extract of green pepper and an effective ingredient, and a health functional food comprising the same.

The health functional food composition of the present invention is effective for prevention and improvement of influenza virus infection by containing an extract of blueberry pepper as an active ingredient.

The term " health functional food "of the present invention means a food prepared and processed by using a raw material or ingredient having useful functionality in the human body according to Law No. 6727 on health functional foods, and" And function of the nutrient for the purpose of obtaining a beneficial effect in health use such as controlling the nutrient or physiological action.

The food composition of the present invention includes all forms such as functional food, nutritional supplement, health food and food additives. Food compositions of this type may be prepared in a variety of forms according to conventional methods known in the art.

For example, as a health food, the green pepper extract itself may be prepared in the form of tea, juice, and drink and then taken for drinking, granulated, encapsulated and powdered. In addition, the health functional food of the present invention can be prepared in the form of a composition by mixing with known active ingredients known to have the effect of preventing or ameliorating a disease or symptom caused by infection with influenza mediated inflammation and influenza virus .

Possible foods also include beverages (including alcoholic beverages), fruits and their processed foods (eg canned fruits, bottled, jammed, maamalade, etc.), fish, meat and processed foods such as ham, sausage, Etc.), breads and noodles (eg udon, buckwheat noodles, ramen noodles, spaghetti, macaroni, etc.), fruit juice, various drinks, cookies, , Retort foods, frozen foods, various seasonings (eg, soybean paste, soy sauce, sauces, etc.). In addition, in order to use the blue pepper extract of the present invention in the form of a food additive, it may be used in the form of powder or concentrate.

The preferred content of the blueberry capsicum extract in the food composition of the present invention may be 0.001 to 50% by weight, preferably 0.1 to 30% by weight, based on the total weight of the food composition.

The present invention relates to an antiinflammatory and anti-influenza virus effect of a green pepper extract, and more particularly to a composition for preventing and treating inflammatory-mediated inflammatory disease or influenza virus infection, The present invention relates to a health functional food having specific characteristics. The present invention is effective in preventing, improving and treating inflammatory mediated inflammatory diseases by using the anti-inflammatory effect of blueberry pepper extract, and also effective in preventing, improving and treating influenza virus infection by using antiviral effect.

Figure 1 shows NLRP3, NLRC4, and AIM2 inflammation activity assessed for blue pepper extract, and the effect was detected by western blotting of the amount of active IL-1β released into cell culture (Sup. Intracellular protein extract, IL-1β active cytokine, pro-IL-1β: precursor cytokine).
Fig. 2 shows the effect of inhibiting influenza by the concentration of blueberry extract. (OD) con-: no influenza virus, con +: influenza virus, cytotoxic: cytotoxic, antiviral activity of influenza: efficacy of influenza ))

Hereinafter, the present invention will be described in detail.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

≪ Example 1 >

Manufacture of blue pepper extract

1000 ml of ethanol (99.99% v / v) was added to 1 kg of sample blue pepper, mixed and allowed to stand at room temperature overnight. The filtrate was collected using a coarse filter paper (Advantec No. 1), and the filtrate was collected and concentrated under reduced pressure at 55-65 ° C. When the ethanol was completely removed, the concentrate was collected and filtered through a micro filter paper (Advantec No. 2). The pH of the filtrate was collected, and the pH was adjusted to 7.4 using 1.0 or 0.1 mol / L sodium hydroxide solution (NaOH). The content of the pH-adjusted filtrate was measured and then adjusted to 100 mg / mL.

≪ Example 2 >

Antiinflammatory effect of blue pepper extract

≪ Example 2-1 > Isolation and culture of mouse bone marrow-derived macrophages

The mouse (C57BL6, orient bio) is euthanized and the femur and tibia are separated. Separate bone is pierced and bone marrow is extracted with a syringe. 5 ml of RBC lysisbuffer (intron biotech, Seoul, Korea) was added to the extracted bone marrow to destroy red blood cells. Bone marrow cells from which red blood cells were removed were differentiated into macrophages for 1 week in DMEM (10% FBS) supplemented with about 30% of L929 (Korean Cell Line Bank) culture. Macrophages (10 ⁶ cells) were post-cultured on a 12-well plate and inflammation activity experiments were performed.

Example 2-2 Inflammose activity evaluation

IL-1β precursor (pro-IL-1β) was produced by treating LPS (10 ug / ml, Sigma-aldrich) for 3 hours. Afterwards, RMPI (Wellgene, Seoul, Korea) was treated with an artificial NLPR3 in flumazo active drug for 1 hour at each concentration. To activate NLRP3 inflammase, ATP was treated at 2 mM or Nigericin (NG) at 40 uM. NLRC4-flight llama is little by infection with Salmonella Typhimurium C1 .5XlO ⁸ cfu) caused an activation. For the activity of AIM2 inflammase, dsDNA (2 ug) was treated with lipofectamine 2000 (4 ul) and transfected into cells. One hour later, the supernatant (Sup) was collected and the intracellular proteins (Lysate, Lys) were extracted.

The collected cultures and intracellular proteins were electrophoresed on PAGE-gel (16% or 10%). The protein is then transferred to a PVDF membrane (Pall Corporation, USA). Membranes were incubated with 3% skimmed milk powder in a solution containing antibody (IL-1β [R & D system] or Actin [Santa Cruz biotech], 1: 1000) overnight. The next day, the cells were washed with PBS and cultured for 3 hours in a secondary antibody (Santa Cruz biotech, 1: 10,000) with HRP. After completion of the reaction, the membrane was washed with PBS and reacted with ECL solution (Philekore), and analyzed using a luminescent assay organ (Ez-Capture, Atto). In order to analyze the results, the efflux activity was evaluated by comparing the amount of active IL-1β released from the cell culture medium containing both the active treatment of the active drug with the active ingredient and the Puerariae Radix extract.

As shown in FIG. 1, the blue pepper extract of the present invention effectively inhibited ATP-induced NLRP3 inflammase at a concentration of 6.25 mg / mL or more and NG-induced NLRP3 inflammase at a concentration of 3.125 mg / mL or more In addition, when the concentration was above 6.25 mg / mL, it effectively inhibited AIM2 inflammase.

≪ Example 3 >

Cytotoxicity (cvtotoxici tv) of blue pepper extract and inhibition effect of influenza virus

≪ Example 3-1 >

Madin-Darby canine kidney epithelial cell (MDCK Line) was used as a cell line. (MDCKcell) was added to each well of a 96-well plate at a concentration of 1.5 × 10 ⁵ cells / mL in a 96-well plate. The cell culture medium (MEM + 10% FSB + 100 U / mL penicillin + 0.1 mg / mL streptomycin) well plates and cell density (cell confluence) was 70% - 80%.

<Example 3-2> Cytotoxicity evaluation

To evaluate the cytotoxicity, the cellulase extract was treated at a concentration ranging from 10.000 mg / mL to 0.0015 mg / mL, followed by culturing the cell line with a 5% CO ₂ incubator at 37 ° C for 48 hours, And the absorbance was measured.

The results of the cytotoxicity evaluation of the blue pepper extract are shown in [Fig. 2]. When the cellulase extract was added to the cell extracts, the absorbance gradually increased as the solids content of the cellulase extract was diluted and decreased. Therefore, the extract of Cheongpongchu showed low cytotoxicity at less than 0.0015mg / mL of solids.

Example 3-3 Influenza Virus Inhibitory Effect

In order to examine the anti-influenza effect, the blue chili extract was treated at concentrations ranging from 10.000 mg / mL to 0.0015 mg / mL and H1N1 (PR8) influenza virus was inoculated as shown in FIG. The cells were cultured in a 5% CO ₂ incubator at 37 ° C for 48 hours, and the surviving cell line was stained with MTT method and absorbance was measured.

The anti-influenza effect of the green pepper extract is shown in Fig. At a concentration of 10.000 mg / mL of blue pepper extract, the absorbance was low and the number of living cell lines was small. On the other hand, the absorbance of living cell line was increased at the diluted concentration of the blue - pepper extract, and the absorbance at the solid concentration of 0.0412 mg / mL of the blue - pepper extract reached a maximum value of 0.9 and showed the effect of suppressing the influenza virus to the maximum.

Accordingly, the present invention relates to an anti-inflammatory and anti-influenza virus effect of blueberry extract, and more particularly to a pharmaceutical composition for preventing and treating influenza-mediated inflammatory diseases and influenza virus infection, . Also disclosed is a health functional food composition for preventing and ameliorating inflammatory-mediated inflammatory disease or influenza virus infection comprising an extract of Chrysanthemum morifolium as an active ingredient, and a health functional food comprising the same. The present invention relates to an anti-inflammatory mediated inflammatory disease and an influenza virus infection prevention using the anti-inflammatory and antiviral effect of the extract of Chili pepper. It has an excellent effect on improvement and treatment, and the possibility of utilization of acid leaf is high.

Claims (12)

A pharmaceutical composition for the prevention and treatment of inflammatory diseases mediated by inflammatory disease, which comprises an extract of Cheong Chu Pung as an active ingredient.
3. The composition of claim 1, wherein said inflammase is NLRP3 inflammase or AIM2 inflammoma.
The method of claim 1, wherein the inflammatory disease is selected from the group consisting of familial Mediterranean fever, hyperimmunoglobulin d syndrome, PAPA syndrome, pyodermagangrenosum, and acne, Muckle-Wells syndrome, neonatal mutisystem inflammatory disease, and cryopyrin-associated periodic syndromes. &Lt; RTI ID = 0.0 &gt;
A health functional food composition for the prevention and improvement of inflammatory diseases mediated by inflammation, which comprises an extract of Cheongpungcho as an active ingredient.
5. The composition of claim 4, wherein said inflammase is NLRP3 inflammase or AIM2 inflammoma.
5. The method of claim 4, wherein the inflammatory disease is selected from the group consisting of familial Mediterranean fever, hyperimmunoglobulin d syndrome, PAPA syndrome (pyrogenic arthritis, pyodermagangrenosum, and acne), Merkle-Wells syndrome Muckle-Wells syndrome, neonatal mutisystem inflammatory disease, and cryopyrin-associated periodic syndromes. &Lt; RTI ID = 0.0 &gt;
A health functional food comprising the food composition of claim 4.
A pharmaceutical composition for the prevention and treatment of influenza virus infection, which comprises an extract of Cheongpungcho as an active ingredient.
A health functional food composition for preventing and improving influenza virus infection, comprising an extract of Cheong Chu Pung as an active ingredient.
10. The composition of claim 8 or 9, wherein the influenza virus is the H1N1 virus.
10. The composition according to claim 8 or 9, wherein the disease caused by the influenza virus infection is flu, cold, sore throat, bronchitis and pneumonia.
A health functional food comprising the food composition of claim 10.

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