KR20160015941A - Method for increasing biomass of Spirulina using light emitting diode irradiation of specific wavelength - Google Patents

Method for increasing biomass of Spirulina using light emitting diode irradiation of specific wavelength Download PDF

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KR20160015941A
KR20160015941A KR1020140098958A KR20140098958A KR20160015941A KR 20160015941 A KR20160015941 A KR 20160015941A KR 1020140098958 A KR1020140098958 A KR 1020140098958A KR 20140098958 A KR20140098958 A KR 20140098958A KR 20160015941 A KR20160015941 A KR 20160015941A
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South Korea
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microalgae
spirulina
led
wavelength
present
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KR1020140098958A
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Korean (ko)
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임성근
권태호
기대원
이정노
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(주)엔비엠
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

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Abstract

The present invention relates to a method for increasing a biomass of microalgae, comprising a step radiating LED light of green light wavelength of 520-530 nm or red light wavelength of 620-670 nm to microalgae by the intensity of 800-2,200 umol photon m^(-2)s^(-1). The mass production of microalgae is industrialized to produce high-value microalgae without influences from weather and environment conditions around the year through developing of an optimal light source irradiation condition for the mass production of microalgae, by using the irradiation of LED light in specific wavelengths, thereby being used in a relating industry.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a method for increasing the biomass of Spirulina using LED illumination of a specific wavelength,

The present invention relates to a method for increasing the biomass of Spirulina using LED irradiation of a specific wavelength, more specifically, a red light wavelength of 520 ~ 530nm green light wavelength or 620 ~ 670nm of the LED in Spirulina 800 ~ 2200umol photon m - 2 s -1 to the intensity and irradiation provides a method for increasing the biomass of Spirulina, including the steps of culturing.

Spirulina, a microalgae, is a prokaryote belonging to the genus Cyanobacterium. Its protein content is very high, 46-71% of its dry weight. It is also found in the genus of gamma-linolenic acid (GLA), phycocyanin, myxoxanthophyll, and zeaxanthin, which are known to be useful as health supplements for providing protein and vitamins to animals as well as humans. Natural ficocyanin present in spirulina is a protein color and has strong and diverse functional properties such as anti-cancer, anti-aging, anti-HIV, anti-inflammatory action and is added to candy, ice cream, dairy product and soft drink for food processing. , Eye line, and lipstick.

Spirulina is a microalgae that grows well in the high alkaline environment (pH 9.5 ~ 11) of the tropical region. It grows through photosynthesis by using sunlight as an energy source like plants. There have been various studies to produce maximum dry weight crude by controlling culture conditions such as incubator, brightness or temperature to produce high biomass of Spirulina. Conventionally, a method of culturing using deep-sea water or SOT medium (Society of Toxicology (SOT) medium) has been developed in the open air or outdoors using sunlight. However, in Korea, there are environmental problems due to temperature and climate change when cultivating spirulina outdoors. Therefore, in order to produce spirulina of the same production quality while maintaining constant light quantity and culture temperature, it must be cultivated in an enclosed space. Since spirulina is a microalgae that grows with light as a carbon source, artificial light sources and methods for producing light have been required.

Accordingly, the present inventors have developed a method for producing high biomass by irradiating LEDs having a specific wavelength during the cultivation of Spirulina strain without changing the composition of the medium to produce high biomass of Spirulina.

Korean Patent No. 10-1256434 discloses 'a method for culturing spirulina using a culture medium for spirulina culture', Korean Patent No. 10-0622025 discloses a culture composition for optimizing growth and maximum harvesting of spirulina, The method of increasing the biomass of Spirulina using LED illumination of a specific wavelength as in the present invention has not been disclosed.

SUMMARY OF THE INVENTION [0005] The present invention has been made in view of the above-mentioned needs, and it is an object of the present invention to provide a SOT medium, which has been conventionally used when cultivating microalga, Spirulina platensis, The present inventors have completed the present invention by confirming that a high yield of biomass can be obtained in a short period of time as a result of culturing the LED at a wavelength of 800 to 2200 μmol photon m -2 s -1 .

In order to solve the above problems, the present invention provides a method for increasing the biomass of microalgae, comprising the step of irradiating microalgae with a light emitting diode (LED).

As described above, by developing optimal light source conditions for mass production of microalgae using LED illumination of a specific wavelength according to the present invention, it is possible to industrially mass-produce microalgae to affect climate and environmental conditions It is possible to produce high-value-added microalgae in the year without receiving them, and thus it can be very usefully used in related industries.

1 is a schematic view schematically showing the structure of a photobioreactor and an LED used in the present invention.
Fig. 2 is a graph showing the relationship between the amount of micro-algae This is a photograph showing an example of culturing Spirulina.
FIG. 3 is a graph showing the dry weight of the microalgae spirulina cultured at each LED wavelength using the photobioreactor of the present invention.

In order to accomplish the object of the present invention, the present invention provides a method for increasing the biomass of microalgae, comprising irradiating and cultivating a microalgae with a light emitting diode (LED).

The term "LED" refers to a light emitting diode. The LED lighting apparatus combines various types of LEDs that emit light of optimum (optimum) wavelength in accordance with the kinds of plants and the growing process, The present invention relates to a lighting apparatus formed by arranging a plurality of LEDs on a member such as a panel or the like. More specifically, one or more LEDs selected from red, blue, green, and white LEDs are arranged on a board .

The term "irradiation" refers to exposing a plant surface to LED light by illuminating the light on the surface.

In the method according to an embodiment of the present invention, the microalgae may be, but is not limited to, Spirulina platensis .

In the method according to an embodiment of the present invention, the LED may be an LED of a green light wavelength or a red light wavelength, preferably a green light wavelength of 520 to 530 nm or an LED of a red light wavelength of 620 to 670 nm, May be an LED having a green light wavelength of 525 nm or a red light wavelength of 630 nm or 660 nm, but is not limited thereto.

In the method according to an embodiment of the present invention, the LED illumination can be irradiated with 800-2200 nm photon m -2 s -1 intensity, preferably 1000-2000 nmol photon m -2 s -1 intensity But is not limited thereto.

In the method according to an embodiment of the present invention, the microalgae may be cultured at 25 to 29 ° C while injecting 4 to 6% (v / v) carbon dioxide at 0.9 to 1.1 L / h, Preferably at 5 [deg.] C (v / v) carbon dioxide at 27 [deg.] C while injecting 1 L / h of carbon dioxide.

The method according to one embodiment of the present invention is a method of irradiating microalgae with a green light wavelength of 520 to 530 nm or a red light emitting diode (LED) having a wavelength of 620 to 670 nm at 800 to 2200 μmol photon m -2 s -1 intensity, And culturing under the conditions of 25 to 29 ° C while injecting 6% (v / v) carbon dioxide at 0.9 to 1.1 L / h. Preferably, microbeds are irradiated with green light having a wavelength of 525 nm or LEDs having a wavelength of 630 nm or 660 nm (v / v) carbon dioxide at 1 L / h while irradiating with a light emitting diode (LED) at a dose of 1000 to 2000 μmol photon m -2 s -1 . .

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Materials and methods

Strain and medium

The strain used in the present invention was purchased from Korean Marine Microalgae Bank (Pukyong National University) as Spirulina platensis CY-007. The medium used in this experiment is a general SOT medium, and the constituents are shown in Tables 1 and 2 below.

General SOT medium composition table used for culture of spirulina seeds Medium component content( mg / 100 mL ) NaHCO 3 1680 K 2 HPO 4 50 NaNO 3 250 K 2 SO 4 100 NaCl 100 MgSO 4 .7H 2 O 20 CaCl 2 .2H 2 O 4 FeSO 4 .7H 2 O One Na 2 EDTA 8 A 5 solution 0.1 mL

The composition of the A 5 solution in the general SOT medium used for the culture of Spirulina seeds ingredient content ( mg / 100 mL ) H 3 BO 3 286 MnSO 4 .7H 2 O 250 ZnSO 4 .7H 2 O 22.2 CuSO 4 · 5H 2 O 7.9 Na 2 MoO 4 .2H 2 O 2.1

Internal survey type Photobiological  Reactor

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to FIG. 1 of the present invention. In the drawings, the same components are denoted by the same reference symbols whenever possible. In the following description, well-known functions or constructions are not described in detail since they would obscure the invention in unnecessary detail.

Fig. 1 is a view showing an internal irradiation type photobioreactor used in the present invention. Fig. 1 shows a reactor 1 having a columnar shape, a column 2 inserted into the center of the reactor 1, A gas supply unit 11 provided below the reaction unit 1 to supply the gas into the reaction unit 1, a flow meter 12 for adjusting the flow rate of the gas, A pump 14 for producing gas, and a filter unit 13 for filtering the produced gas. A sampling port 10 is formed at the lower end or upper end of the reaction part 1 to inject a photosynthetic biological culture liquid or to sample a photosynthetic biological culture liquid therein. The illumination unit 3 inserted into the column unit 2 is composed of a plurality of LEDs. At this time, the wavelength of the LED of the illumination unit 3 may be 450 to 660 nm. The illuminating unit 3 includes an external power source is connected and operated via the external power supply (8), 50 ~ 2000 umol photon m -2 s - a module (9) that can adjust the amount of light is connected to one. A cooling device 7 may be installed in the column part 2 where the illumination part 3 is inserted to cool the heat that may be generated when the photosynthetic organism inside the reaction part 1 grows. The cooling device 7 cools only the inside of the column so as not to affect the temperature inside the reaction part 1. The cooling device 7 is configured to cool the inside of the column part 2 by inserting a circulation tube 6 through which water or cool air is circulated into the inside 5 of the column part 2 or to cool the inside of the column part 2 The inside of the column section 2 can be cooled by introducing cool air. The upper part of the reaction part 1 is sealed with the upper cap 4 to prevent contamination of the culture liquid in a state where the column part 2 and the illumination part 3 are inserted into the reaction part 1. The internal irradiation type photobioreactor according to the above structure is 55 cm in height and 15 cm in diameter inside the reactor.

Example  1. At a specific wavelength LED As a light source Spirulina  Platensis Spirulina platensis )of  Comparison of dry weight after culture

In a 1 L Erlenmeyer flask, 600 mL of SOT medium was added, 10 days of spirulina cells cultured for 10 days were inoculated, and the mixture was incubated for 10 days with shaking at 100 rpm at 27 ° C with light of 20 umol photon m -2 s -1 intensity for 24 hours. (White 6500K), blue (450nm and 460nm), green (525nm) and red (630nm and 660nm), respectively, were inserted into the seven internal irradiation type photobioreactors shown in Fig. 1, Was used as the photocatalytic reactor. 400 mL of the cultured medium for 10 days in an agitation incubator was inoculated into 4 L SOT medium at a concentration of 10% (v / v). Next, each photobioreactor was wrapped with aluminum foil to block external light from entering, and the amount of LED light of each wavelength was adjusted to 4 conditions (200, 500, 1000 and 2000 umol photon m -2 s -1 ) , And cultured at a temperature of 27 ° C while flowing 5% (v / v) of carbon dioxide at 1 L / h. All the culture solutions were sampled in 40 mL increments at 24 hour intervals according to the incubation time, and the dry weight was measured. The results were shown in FIG. 2 and FIG. 3 by observing with a microscope.

In order to measure the dry weight of Spirulina cultured by irradiating LEDs of respective wavelengths, the culture solution collected at intervals of 24 hours for 10 days of culture was preliminarily dried and filtered with a filter paper whose weight was measured before filtration, After drying for 1 hour at 60 ° C, the weight was measured and the dry weight was calculated by the weight difference between before and after the filtration. As a result, it was not able to grow when there was no carbon source, and it was confirmed that the amount of biomass increased as the amount of LED of each wavelength increased. However, the growth rate and the amount of biomass are different depending on the light source LED. The blue LED (450 nm and 460 nm) shows little growth at 500 μmol photon m -2 s -1 or lower, and 1000 μmol photon m -2 s -1 or higher And other red, green, and white LEDs were able to produce about 1 g / L spirulina biomass (dry weight) when cultured for 10 days at 1000 μmol photon m -2 s -1 or higher .

As described above, the present invention has an advantage in mass production of biomass of photosynthetic microorganisms using LEDs having a specific wavelength.

1: Reaction part
2: Column part
3:
4: Upper cap
5: Inside of column
6: circulating tube
7: Cooling unit
8: External power source
9: Module
10: Collecting hole
11:
12: Flowmeter
13:
14: Pump

Claims (7)

A method for increasing the biomass of a microalgae, comprising irradiating microalgae with an LED (light emitting diode). 2. The method of claim 1, wherein the microalgae is Spirulina platensis . The method of claim 1, wherein the LED is an LED having a green light wavelength or a red light wavelength. 4. The method of claim 3, wherein the LED is an LED having a green light wavelength of 520 to 530 nm or a red light wavelength of 620 to 670 nm. The method of claim 1, wherein the LED illumination is irradiated at 800-2,200 umol photon m -2 s -1 intensity. The microalgae of claim 1, wherein the microalgae is cultivated at 25 to 29 ° C while injecting 4 to 6% (v / v) of carbon dioxide at 0.9 to 1.1 L / h. / RTI > The method of claim 1, further comprising irradiating the microalgae with 800-2200 nm photon m -2 s -1 intensity of a green light having a wavelength of 520-530 nm or a red light having a wavelength of 620-670 nm, / v) culturing under conditions of 25 to 29 DEG C while injecting carbon dioxide at 0.9 to 1.1 L / h.
KR1020140098958A 2014-08-01 2014-08-01 Method for increasing biomass of Spirulina using light emitting diode irradiation of specific wavelength KR20160015941A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11473051B2 (en) 2019-02-27 2022-10-18 Nichia Corporation Method of cultivating algae and photobioreactor

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11473051B2 (en) 2019-02-27 2022-10-18 Nichia Corporation Method of cultivating algae and photobioreactor

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