KR20150108065A - Cosmetic composition containing extracts of solanum melongena and/or lagenaria leucantha - Google Patents

Abstract

The present invention relates to a cosmetic composition and, more specifically, to a cosmetic composition containing an extract of Solanum melongena and/or Lagenaria leucantha, which minimizes repulsion of customers and side effects of compounds, and has anti-oxidant and anti-inflammatory effects. The cosmetic composition according to the present invention contains one or more kinds selected from a group consisting of a Solanum melongena extract and a Lagenaria leucantha extract as active ingredients. The cosmetic composition according to the present invention not only has less side effects to a human body, but also has excellent anti-oxidant and anti-inflammatory effects, thereby being useful in cosmetics, skin medicines, etc.

Description

COSMETIC COMPOSITION CONTAINING EXTRACTS OF SOLANUM MELONGENA AND / OR LAGENARIA LEUCANTHA [0002]

The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition containing a branch and / or a foaming extract having antioxidant and anti-inflammatory effects, minimizing the rejection and side effects of the consumer on the composition.

Skin is an important tissue that has various functions and protects the human body from the external environment in direct contact with the external environment. Melanin is a pigment present in the epidermis of these skin tissues. It is made from melanocytes in the basal layer of the epidermis and moves to epidermal cells called keranosites. In vivo, there is no enzymes that degrade melanin, but keratinocytes are removed from the skin as they fall off the epidermis. Melanin not only determines the skin color of a person but also plays a role of skin protection (free radical scavenger). However, when melanin is formed in the skin due to external environmental changes such as excessive exposure to ultraviolet rays, air pollution, stress, Which causes skin color to become black or cause spiny freckles and the like.

The melanin synthesis process uses tyrosine as a substrate and tyrosinase, an enzyme called DOPAquinone, which undergoes autooxidation and enzymatic reaction from dopaquinone to produce melanin, a copolymer. The resulting melanin is transferred to the keratinocyte through a bag called melanosome, and is then exposed to the skin surface through a keratinocyte keratinization process for 28 days. However, in this process, melanin is excessively produced by a factor promoting melanin production, and when melanin is not completely eliminated by keratinization, a pigmentation phenomenon appears. Therefore, in order to prevent such pigment deposition phenomenon, it can be suppressed by controlling some processes in the melanin production process.

 There are many mechanisms that inhibit melanin synthesis. A typical synthesis inhibition process inhibits tyrosinase, an important enzyme of melanin synthesis. The most known chelator is kojic acid, and there are substrate-like substances such as arbutin. In addition, there are hydroquinone, vitamin A and the like, and vitamin C, a representative antioxidant, and derivatives thereof. However, when cosmetics are manufactured using the above-mentioned components, there is a disadvantage in that the color of cosmetics is discolored due to oxidation by the reaction with light during the manufacturing process or storage of the product. Therefore, additives such as antioxidants, UV absorbers, and metal chelating agents were added to reduce such discoloration. However, when a large amount of such an additive is used, there is a problem in skin stability, such as irritating the skin, and a sufficient whitening effect can not be obtained as much as desired.

All aerobic organisms, including humans, use oxygen to metabolize energy and survive. However, if the in vivo oxygen is subjected to various physical, chemical and biological stresses, it becomes a harmful reactive oxygen species such as superoxide anion radical, hydrogen peroxide, and hydroxyl radical, causing a deadly physiological disorder to the human body, causing serious disease and causing loss of life . At present, antioxidants have been made and used as chemical compounds by economics. However, vitamin E derivatives, vitamin C and flavonoids have poor stability and safety and are hardly used as cosmetic raw materials.

Choi et al., KSBB Journal, 2009

It is an object of the present invention to provide a cosmetic composition and a method of manufacturing the same that minimizes the rejection and side effects of consumers on a composition and has an antioxidant and anti-inflammatory effect.

The object of the present invention can be attained by providing a cosmetic composition containing at least one selected from the group consisting of eggplant extracts and peach extract as an active ingredient.

According to a preferred feature, the cosmetic composition may contain, as an active ingredient, 0.1 to 30% by weight of the egg extract or 0.1 to 30% by weight of the nut extract relative to the whole composition.

According to another preferred characteristic, the eggplant extract may be a solvent extract obtained from at least one extraction solvent selected from the group consisting of water and a fermentation alcohol.

According to another preferred feature, the foaming extract may be a foaming solvent extract obtained by extracting foil with at least one extraction solvent selected from the group consisting of water and fermented spirits.

According to another preferred characteristic of the present invention, the cosmetic composition according to the present invention is used as an external ointment, essence, lotion, cream, gel, lotion, pack, massage cream, milky lotion, foundation, makeup base, soap, liquid detergent, Oils and the like.

The cosmetic composition according to the present invention not only has a small adverse effect on the human body but also has excellent antioxidant and anti-inflammatory effects and can be used for cosmetics and skin medicines.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing free radical scavenging activity according to the concentrations (250 ppm, 500 ppm, 1000 ppm, and 5000 ppm) of the egg plant extracts and the night extracts of Examples 1 and 2;
FIG. 2 is a graph showing the free radical scavenging activity according to the concentrations (250 ppm, 500 ppm, and 1000 ppm) of the egg plant extracts and the night extracts of Examples 1 and 2;
FIG. 3 is a graph showing inhibitory activity of tyrosinase activity according to the concentrations (100 ppm, 500 ppm, and 1000 ppm) of the egg plant extract and the night extract of Examples 1 and 2;
FIG. 4 is a graph showing the inhibition of L-DOPA oxidation according to the concentrations (100 ppm, 500 ppm, and 1000 ppm) of the egg-plant extracts and the night extracts of Examples 1 and 2;
FIG. 5 is a graph showing cell viability according to the concentrations (50 ppm, 100 ppm, and 200 ppm) of the egg plant extracts and the night extracts of Examples 1 and 2;
Fig. 6 is a graph showing the cellular melanin content (% of control) according to the concentrations (50 ppm, 100 ppm, 200 ppm) of the egg plant extracts and the night extracts of Examples 1 and 2;

Hereinafter, the present invention will be described in detail.

The cosmetic composition according to the present invention contains at least one kind selected from the group consisting of eggplant extract and peach extract as an active ingredient, and the cosmetic composition has antioxidative or whitening effect.

Here, the branch is a year-round herbaceous herbaceous plant with a monocotyledonous plant, and its scientific name is Solanum melongena , which is characterized by being cultivated from tropical to temperate.

In addition, the annual plant a vine full of foil and dicotyledon cucurbitales night indeed gourd, watermelon vine, also called pyogwa, the scientific name Lagenaria leucanth . It is 10cm long with a bluish green color. It has short hairs and petiole.

The branches and foils applicable to the present invention may be wild or cultivated, and can be used in a dried or non-dried state, and the extraction site is not particularly limited.

In order to obtain the egg plant extract, the extraction solvent may be at least one kind selected from the group consisting of water, fermented juice, etc., preferably fermented juice, or can be mixed for daily. For example, water and fermented juice may have a volume ratio of 1:50 To 50: 1. ≪ / RTI > The amount of the extraction solvent to be used is preferably 1 to 30 times by volume (for example, using 1 to 30 mL of solvent per 1 g of branch), preferably 2 to 10 times by volume . The extraction time may be, but is not limited to, 1 to 12 hours, preferably 3 to 6 hours for sufficient extraction of the active ingredient. The extraction temperature is not limited thereto, but may range from room temperature to the boiling point of the extraction solvent for effective extraction of the active ingredient. The extracting method for obtaining the eggplant extracts can be produced according to known extraction methods commonly used in the technical field of the present invention. Specifically, a commonly used extraction method such as a cold precipitation method, a heating extraction method, a pressure extraction method and a reflux extraction method may be used. Further, if necessary, filtration and / or concentration and / or lyophilization methods known in the art can be additionally used after the extraction, if necessary.

The extractant may be at least one kind selected from the group consisting of water, fermented juice, etc., preferably one or more solvents selected from the group consisting of water and fermented juice, or a mixture thereof. For example, water and fermentation broth may be mixed daily in the range of 1: 50 to 50: 1 by volume. The amount of the extraction solvent to be used is preferably 1 to 30 times by volume (for example, using 1 to 30 mL of solvent per 1 g of foil), preferably 2 to 10 times by volume based on the weight of the foil, . The extraction time and extraction method are as described above.

The content of the extract as an active ingredient in the cosmetic composition can be appropriately adjusted for whitening or antioxidative effect. For example, the cosmetic composition may contain, as an active ingredient, 0.1 to 30% by weight of the egg extract or 0.1 to 30% by weight of the night extract, preferably 1 to 30% by weight of the egg extract or 1 to 30% By weight, most preferably 1 to 10% by weight of the egg branch extract or 1 to 10% by weight of the nut extract as an active ingredient. That is, the content of the extract is preferably not less than 0.1% by weight so as to attain the least antioxidative or whitening effect, and the content of the extract is not more than 30% by weight in consideration of the deterioration of the feeling of use due to the excessive addition and the applicability to various formulations .

The cosmetic composition according to the present invention can be used for external ointment for skin, basic cosmetics, makeup cosmetics, and body cosmetics. Examples of the basic cosmetics include essences, lotions, creams, gels, lotions, packs, massage creams, milky lotions and the like. Examples of the makeup cosmetics include foundation, makeup base and the like. Examples of the body cosmetics include soaps, liquid detergents, Sunscreen cream, sun oil, and the like.

At this time, fragrance, dye, fungicide, preservative, humectant, thickener, inorganic salt, synthetic polymer material and the like may be further added to the composition for improving the physical properties.

In addition, the cosmetic composition is characterized in that the extract does not show cytotoxicity, and thus has no adverse effects on the human body.

The method for producing a cosmetic composition according to the present invention comprises the steps of extracting a branch extract by adding at least one solvent selected from the group consisting of water, fermented juice, and the like to a branch or adding one kind selected from the group consisting of water, fermented juice, Or more, and extracting it with a solvent to obtain a foaming extract.

In addition, the step of obtaining the extract may be performed once or two or more times, for example, two or three times, and the filtrate obtained in each extraction step may be combined to obtain an extract.

The extraction can be carried out according to all known methods commonly used in the technical field of the present invention and can be carried out by a commonly used extraction method such as a cold precipitation method, a heat extraction method, a pressure extraction method and a reflux extraction method .

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. It is to be understood, however, that the embodiments described below are only for explanation of the embodiments of the present invention so that those skilled in the art can easily carry out the invention, It does not mean anything.

Example  1. Preparation of eggplant extract

After drying, the dried branches (Good Branch Farm, Yeongju, Gyeonggi Province) were cut into a grinder, and then heated in a 70% fermented juice at 70 ° C for 6 hours. After that, the extract was filtered using a filter aid, and then lyophilized to prepare a branched extract.

Example  2. Preparation of night extract

The dried bacon (Park, Shibak Farm, Chungnam Budget) was cut into a crusher, and then put in a 70% fermented alcohol and heated at 70 ° C for 6 hours. After that, the extract was filtered using a filter aid, and then lyophilized to prepare a foaming extract.

Experimental Example  One. DPPH assay

To investigate the antioxidative effects of the extracts of eggplant and pan prepared in Examples 1 and 2, extracts of different concentrations were tested for free radicals (1,1-diphenyl-2-picryl-hydrazyl) free radical scavenging activity was measured, and ascorbic acid (L-ascorbic acid) was used as a positive control. The results are shown in FIG.

1 is a graphical representation of the free radical scavenging activity according to the concentrations of the extracts of Examples 1 and 2 (250 ppm, 500 ppm, 1000 ppm, 5000 ppm), wherein L-A means ascorbic acid.

As shown in FIG. 1, at 5,000 ppm, the branched extract showed a free radical scavenging activity of 80% or more as compared with the positive control, and the root extract showed 50% free radical scavenging activity as compared with the positive control.

Experimental Example  2. ABTS assay

In order to examine the antioxidant activity of the extracts of eggplant and pan prepared in Examples 1 and 2, free radical scavenging activity was measured by ABTS radical cation decolorization of each of the extracts with different concentrations, (L-ascorbic acid) was used as the control, and the control group was used as the control group. The results are shown in FIG.

Figure 2 is a graphical representation of the free radical scavenging activity according to the concentrations of the extracts of Examples 1 and 2 (250 ppm, 500 ppm, 1000 ppm), where L-A means ascorbic acid.

As shown in FIG. 2, the extracts of leaves of branches showed a 50% or more free radical scavenging activity in a similar pattern to the DPPH assay, and showed a free radical scavenging activity of 30% or more in the case of the night extract.

Experimental Example  3. Determination of polyphenol and flavonoid content

The contents of polyphenols and flavonoids of the extracts of Examples 1 and 2 were measured using Folin-Ciocalteau method and Davis method, respectively, and the results are shown in Table 1 below.

division Polyphenol content (mg / L) Polarbonoid (mg / L) Eggplant extract 139.27 952 Peach extract 220.18 540.89

As shown in Table 1, the polyphenol contents of the eggplant and foil extracts were 139.27 mg / L and 220.18 mg /, respectively, and the flavonoid contents were 952 mg / L and 540.89 mg / L, respectively.

Experimental Example  4. Tyrosinase  Activity inhibition assessment

4.1

In order to examine the whitening efficacy of the extracts of Examples 1 and 2, the inhibitory activity of mushroom tyrosinase activity of the egg-plant extracts and night extracts prepared in Examples 1 and 2 was evaluated. Specifically, 1 mM L-tyrosine and 50 mM potassium phosphate buffer (pH 6.5) were mixed, and the eggplant extract and pestle extract and mushroom tyrosinase were added and reacted at 37 ° C for 10 to 15 minutes. , And the results are shown in FIG.

4.2

In order for melanin to be produced, tyrosinase acts on tyrosine in the cell to generate dopaquinone, which is spontaneously reacted with dopaquinone and enzymatically reacted to produce melanin as a black pigment.

Therefore, in order to evaluate inhibition of tyrosinase activity in relation to the melanin production, inhibition of tyrosinase activity was evaluated by varying the concentrations of the egg extracts and the night extracts of Examples 1 and 2. Specifically, 660 μl of 0.1 M Tris-HCl buffer (pH 6.5) was added to a test tube and maintained at 37 ° C., and 60 μl of 1.5 mM Tyrosine, 60 μl of enzyme solution (Mushroom tyrosinase) And albutine, which is widely used as a whitening cosmetic preparation, was used as a control group, and the control group was used as a control group. The results are shown in FIG.

Fig. 3 shows the inhibitory activity of tyrosinase activity according to the concentrations (100 ppm, 500 ppm, and 1000 ppm) of the egg plant extracts and the night extracts of Examples 1 and 2.

As shown in FIG. 3, at 1000 rpm, 69% of tyrosinase activity was inhibited in the case of eggplant extract and 60% in tyrosinase inhibitory activity in the case of the night extract, showing a tyrosinase activity similar to that of the positive control.

Experimental Example  5. L- DOPA  Evaluation of oxidation inhibition

The antioxidant activity of the extracts of Examples 1 and 2 was inhibited by the addition of 760 μl of 0.1 M Tris-HCl buffer (pH 7.0) in a test tube, and the cells were maintained at 37 ° C., and 190 μl of 10 mM L-DOPA, 50 μl of Mushroom tyrosinase, The absorbance change was measured at 475 nm by adding 40 ul of the sample to be measured for whitening activity. Arbutin, which is widely used for the whitening cosmetic, was used as a control group, and the control group was used as a control.

FIG. 4 shows L-DOPA oxidation inhibitory effects according to the concentrations (100 ppm, 500 ppm, and 1000 ppm) of the egg-plant extracts and the night extracts of Examples 1 and 2.

As shown in FIG. 4, when the concentration was 1000 ppm, the extracts of the eggplant extract, 28%, and the albutin, respectively, were inhibited by 38%, 28% and 51%, respectively.

Experimental Example  6. Assessment of Melanogenesis Cytotoxicity

B16 / F10 cells distributed from Korean Cell Line Bank were cultured in DMEM (Lonza) medium containing penicillin / streptomycin (GIBCO) 1% and fetal bovine serum (GIBCO) 10% C, 5% CO ₂ incubator. In order to evaluate the cytotoxicity, the egg extracts and extracts of Examples 1 and 2 were treated with MTT assay, and arbutin was used as a positive control. The control group was the control group, and the results are shown in FIG.

FIG. 5 shows cell viability according to the concentrations (50 ppm, 100 ppm, and 200 ppm) of the egg branch extract and the night extract of Examples 1 and 2.

As shown in Fig. 5, no cytotoxicity was observed in the melanin-producing cells of B16 / F10 cells of the egg branch extract, the foil extract and the positive control group arbutin at a concentration of 50 to 200 ppm. It was confirmed that the extracts of eggplant and chestnuts could be used as physiologically active materials with relatively low toxicity and corrected for stability.

Experimental Example  7. Assessment of intracellular melanin production inhibition

To investigate the effect of melanin on intracellular melanin production, B16 / F10 cells were cultured in a 6-well plate for 24 hours. The cells were cultured for 3 days after treatment with the samples (Examples 1 and 2) and washed with PBS (phosphate buffered saline) 1N NaOH (sodium hydroxide) solution containing DMSO (dimethyl sulfoxide) was treated and reacted at 80 ° C for 1 hour, and absorbance was measured at 475 nm. The control group was the control group, and the results are shown in FIG.

FIG. 6 shows cellular melanin content (% of control) according to the concentrations (50 ppm, 100 ppm, and 200 ppm) of the egg plant extract and night extract of Examples 1 and 2, and the inhibitory effect on intracellular melanin production Is calculated using the following equation (1).

[Equation 1]

(%) = (Absorbance at the time of adding sample / absorbance of control) x 100

As shown in FIG. 6, melanin synthesis was reduced by 30% or more in the case of the eggplant extract and 200% in the melanin synthesis in the case of the night extract.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments.

Claims (5)

Wherein the composition comprises at least one member selected from the group consisting of eggplant extracts and root extracts as an active ingredient. The method according to claim 1,
Wherein the cosmetic composition comprises 0.1 to 30% by weight of a branched extract or 0.1 to 30% by weight of a thin extract as an active ingredient with respect to the whole composition. The method according to claim 1,
Wherein the branch extract is a solvent extract obtained from at least one extraction solvent selected from the group consisting of water and fermented spirits. The method according to claim 1,
Wherein the foaming extract is a foaming solvent extract obtained by extracting foil with at least one extraction solvent selected from the group consisting of water and fermented spirits. The method according to claim 1,
The cosmetic composition is selected from the group consisting of external ointment, essence, lotion, cream, gel, lotion, pack, massage cream, milky lotion, foundation, makeup base, soap, liquid detergent, bath agent, sunscreen cream, ≪ / RTI >

Images