KR20150099630A - Method for manufacturing fermented liquor - Google Patents

Method for manufacturing fermented liquor Download PDF

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KR20150099630A
KR20150099630A KR1020140020066A KR20140020066A KR20150099630A KR 20150099630 A KR20150099630 A KR 20150099630A KR 1020140020066 A KR1020140020066 A KR 1020140020066A KR 20140020066 A KR20140020066 A KR 20140020066A KR 20150099630 A KR20150099630 A KR 20150099630A
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vegetable
fruit
vegetables
fruits
fermentation broth
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KR1020140020066A
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Korean (ko)
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정후길
박종혁
허창기
최희영
오전희
양희선
김경희
최유진
이주희
문혜정
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재단법인 임실치즈과학연구소
임실군
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Priority to KR1020140020066A priority Critical patent/KR20150099630A/en
Publication of KR20150099630A publication Critical patent/KR20150099630A/en

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/133Fruit or vegetables
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/003Control or safety devices for sterilisation or pasteurisation systems
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/36Freezing; Subsequent thawing; Cooling
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/40Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Microbiology (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
  • Dairy Products (AREA)

Abstract

The present invention relates to a method for preparing a vegetable/fruit fermentation liquid, and to a method for preparing fermented milk and fermented pudding using the fermentation liquid. More specifically, the method for preparing a vegetable/fruit fermentation liquid comprises: a washing step of washing fruits/vegetables; a heating and sterilizing step of heating the washed vegetables/fruits at 100°C for 20 seconds, and sterilizing the vegetables/fruits to remove bacteria and a colon bacillus group contained in raw materials; a cooling step of cooling the heated and sterilized vegetables/fruits; a lactobacillus strain inoculation step of inoculating the cooled vegetables/fruits with a lactobacillus strain, and preventing polyphenol components from being destroyed; and a low temperature aging step of aging the vegetables/fruits inoculated with the lactobacillus strain at 25°C for 7 days.

Description

야채/과일 발효액 제조방법과 상기 발효액을 이용한 발효유 및 발효푸딩의 제조방법{METHOD FOR MANUFACTURING FERMENTED LIQUOR}METHOD FOR MANUFACTURING FERMENTED LIQUOR [0002] This invention relates to a method for producing a fermented milk,

본 발명은 야채/과일 발효액 제조방법과 상기 발효액을 이용한 발효유 및 발효푸딩의 제조방법에 관한 것으로, 더욱 상세하게는 야채/과일 중 두 가지 이상을 선택하여 세절하고 혼합하는 단계와; 상기 혼합된 야채/과일을 100℃로 20초간 가열하여 원료 내에 함유된 세균, 대장균군을 살균하는 가열살균단계와; 상기 가열살균된 야채/과일을 냉각하는 냉각단계와; 상기 냉각된 야채/과일에 유산균주를 접종하여 폴리페놀성분의 파괴를 방지하는 유산균주 접종단계; 및 상기 유산균주가 접종된 야채/과일을 25℃로 7일간 숙성하는 저온숙성단계;를 포함하여 이루어지는 것을 특징으로 하는 야채/과일 발효액 제조방법과 상기 발효액을 이용한 발효유 및 발효푸딩의 제조방법에 관한 것이다.The present invention relates to a method for producing a vegetable / fruit fermentation broth, a fermented milk using the fermentation broth, and a method for producing the fermented milk and fermented pudding. More particularly, Heating the mixed vegetable / fruit at 100 DEG C for 20 seconds to sterilize bacteria and coliform bacteria contained in the raw material; A cooling step of cooling the heat-sterilized vegetable / fruit; A step of inoculating lactic acid bacteria to prevent the destruction of the polyphenol component by inoculating the cooled vegetable / fruit with the lactic acid bacteria; And a low temperature aging step of aging the vegetable / fruit inoculated with the lactic acid bacteria strain at 25 DEG C for 7 days, and a fermented milk and a fermented pudding preparation method using the fermentation broth .

현대인의 건강강한 삶을 영위하기 위해서는 매일 최소한의 과일 두 조각과 다섯 종류의 야채를 섭취함으로써 성인병을 예방하며, 과일과 야채를 거의 먹지 않는 사람들과 비교해 보면 성인병에 걸릴 확률이 더 낮은 것으로 보고되어 있다. Modern people's health To maintain a strong life, daily intake of at least two fruits and five kinds of vegetables to prevent adult diseases, and compared to people who rarely eat fruits and vegetables, it is reported that they are less likely to develop adult diseases .

야채와 과일에는 인체의 생리활성을 조절해주는 파이토케미컬이 다량함유되어 있으며, 파이토케미컬은 파이토(phyto)는 식물, 케미컬(chemical)은 화합물이란 뜻으로 식물 화합물을 일컫는다. 이는 식물 속에 존재하는 성분들 중에서 건강에 유익한 생리활성을 가지는 미량의 성분으로 식물이 병원균, 해충, 곰팡이 등으로부터 자신을 보호하기 위해 생성하는 여러 보호 물질이 바로 파이토케미컬이다. 파이토케미컬은 그 종류가 다양하고 각종 채소나 과일에 각기 다른 성분으로 존재하고 대부분 폴리페놀 형태이다. Vegetables and fruits contain a large amount of phytochemicals that regulate the body's physiological activity. Phytochemicals refers to phyto, plant and chemical compounds refer to plant compounds. This is a trace element that has a physiological activity beneficial to health out of the components present in plants. Phytochemicals are various protective substances that plants produce to protect themselves from pathogens, insects and fungi. Phytochemicals vary in their variety and are found in different types of vegetables and fruits, mostly polyphenols.

토마토, 사과, 수박, 딸기, 자두 등 홍색 식품에는 라이코펜, 안토시아닌 등의 성분이 존재하며, 녹색채소인 블로콜리, 키위, 포도, 아보카도 등은 루테인, 제사틴, 인돌의 성분이, 블루베리, 포도, 가지, 검은콩, 보라색 양배추 등 안토시아닌이, 망고, 파파야, 파인애플, 오렌지, 고구마, 당근 등의 황색식품에는 카로틴 및 베타 카로틴 등의 성분이 존재한다. Lycopene and anthocyanin are present in red foods such as tomatoes, apples, watermelons, strawberries and plums. Lycopene, kiwi, grapes, and avocados are green vegetables. Lutein, Anthocyanins such as eggplant, black beans, and purple cabbage, and yellow foods such as mangoes, papaya, pineapples, oranges, sweet potatoes and carrots contain carotene and beta-carotene.

야채와 과일에 들어있는 파이토케미컬은 수확되는 순간부터 손실되기 시작하며, 가공에 의한 가열처리시 대부분이 파괴되어 실제로는 영양가 제로의 실속 없는 칼로리로 섭취될 수 있다. 또한 특정 색의 야채나 과일만 즐겨 먹는다면 많은 양을 섭취하여도 여전히 결핍되는 영양소가 있으며, 여러 가지 색의 과일과 야채를 골고루 섭취하여야 한다. 또한 야채나 과일의 생리활성 물질은 열에 불완전하여 대부분이 파괴되어 그 효과가 반감이 된다.The phytochemicals contained in vegetables and fruits begin to be lost from the moment they are harvested, and most of them can be destroyed by heat treatment, which can actually be consumed as a non-stoichiometric calorie. Also, if you only eat vegetables or fruits of a specific color, you will still have a deficient nutrient even if you consume large quantities, and you should consume various colors of fruits and vegetables evenly. In addition, physiologically active substances of vegetables and fruits are incomplete due to heat, and most of them are destroyed, and the effect is halved.

등록특허공보 제10-0185250호(유산균이 함유된 천연과채음료의 제조방법)Patent Registration No. 10-0185250 (Method for producing natural fruit and vegetable beverages containing lactic acid bacteria)

본 발명은 야채, 과일 가공시 파괴되는 폴리페놀성분을 유산균(Lactobacillus plantarum)으로 발효하여 폴리페놀성분의 파괴를 억제하며, 발효유 제조에 접목함으로써 현대인에게 부족한 미네랄, 비타민과 함께 폴리페놀이 다량함유되어 있는 야채/과일 발효액의 제조방법을 제공하고자 한다. The present invention relates to a method for fermenting a fermented milk by fermenting a polyphenol component destroyed during processing of vegetables and fruits with lactic acid bacteria ( Lactobacillus plantarum) to inhibit the breakdown of the polyphenol component, To provide a method for producing a vegetable / fruit fermentation broth.

또한, 본 발명의 야채/과일 발효액 제조방법에 따른 발효액을 이용한 발효유 및 발휴푸딩의 제조방법을 발효유 및 발효푸딩의 제조방법을 제공하고자 한다. Also, the present invention provides a method for producing fermented milk and pudding using a fermentation broth according to the method for producing a fermented vegetable / fruit, and a method for producing the fermented milk and the fermented puddle.

상기와 같은 목적을 위하여 본 발명에 따른 야채/과일 발효액 제조방법은 야채/과일 중 두 가지 이상을 선택하여 세절하고 혼합하는 단계와; 상기 혼합된 야채/과일을 100℃로 20초간 가열하여 원료 내에 함유된 세균, 대장균군을 살균하는 가열살균단계와; 상기 가열살균된 야채/과일을 냉각하는 냉각단계와; 상기 냉각된 야채/과일에 유산균주를 접종하여 폴리페놀성분의 파괴를 방지하는 유산균주 접종단계; 및 상기 유산균주가 접종된 야채/과일을 25℃로 7일간 숙성하는 저온숙성단계;를 포함하여 이루어지는 것을 특징으로 한다.In order to achieve the above object, the present invention provides a method of preparing a vegetable / fruit fermentation broth, Heating the mixed vegetable / fruit at 100 DEG C for 20 seconds to sterilize bacteria and coliform bacteria contained in the raw material; A cooling step of cooling the heat-sterilized vegetable / fruit; A step of inoculating lactic acid bacteria to prevent the destruction of the polyphenol component by inoculating the cooled vegetable / fruit with the lactic acid bacteria; And a low temperature aging step of aging the vegetable / fruit inoculated with the lactic acid bacteria strain at 25 DEG C for 7 days.

또한, 본 발명에서 상기 야채/과일은 양배추, 토마토, 브로콜리, 당근, 복분자, 사과, 배 및 레몬이 동일한 양으로 혼합된 것을 특징으로 한다.In the present invention, the vegetable / fruit is characterized in that cabbage, tomato, broccoli, carrot, brambles, apple, pear and lemon are mixed in the same amount.

또한, 본 발명에서 상기 유산균주는 Lactobacillus plantarum인 것을 특징으로 한다. Further, in the present invention, the lactic acid bacteria may be Lactobacillus plantarum .

또한, 본 발명에 따른 야채/과일 발효액을 이용한 발효유의 제조방법은 야채/과일을 세척하는 세척단계; 상기 세척된 야채/과일을 100℃에서 20초간 가열하여 원료 내에 함유된 세균, 대장균군을 살균하는 가열살균단계; 상기 가열살균된 야채/과일을 냉각하는 냉각단계; 상기 냉각된 야채/과일에 유산균주를 접종하여 폴리페놀성분의 파괴를 방지하는 유산균주 접종단계; 상기 유산균주가 접종된 야채/과일을 25℃에서 7일간 숙성하는 저온숙성단계;를 거쳐 제조되는 발효액을 원유에 첨가하고 발효시켜 제조하는 것을 특징으로 한다.In addition, the method for producing fermented milk using the vegetable / fruit fermentation broth according to the present invention comprises a washing step of washing vegetables / fruits; Heating the washed vegetable / fruit at 100 DEG C for 20 seconds to sterilize the bacteria and coliform bacteria contained in the raw material; A cooling step of cooling the heat-sterilized vegetable / fruit; A step of inoculating lactic acid bacteria to prevent the destruction of the polyphenol component by inoculating the cooled vegetable / fruit with the lactic acid bacteria; And fermenting the fermentation broth prepared through the low temperature aging step of aging the vegetable / fruit inoculated with the lactic acid bacteria strain at 25 캜 for 7 days.

또한, 본 발명에 따른 야채/과일 발효액을 이용한 발효푸딩의 제조방법은 야채/과일을 세척하는 세척단계; 상기 세척된 야채/과일을 100℃에서 20초간 가열하여 원료 내에 함유된 세균, 대장균군을 살균하는 가열살균단계; 상기 가열살균된 야채/과일을 냉각하는 냉각단계; 상기 냉각된 야채/과일에 유산균주를 접종하여 폴리페놀성분의 파괴를 방지하는 유산균주 접종단계; 상기 유산균주가 접종된 야채/과일을 25℃에서 7일간 숙성하는 저온숙성단계;를 거쳐 제조되는 발효액을 원유에 첨가하고 발효시켜 제조되는 발효유를 발효유를 물과 젤라틴을 혼합한 후 냉각시킨 젤라틴 용액에 첨가한 후 냉각시켜 제조하는 것을 특징으로 한다. Also, the method for producing fermented puddings using the vegetable / fruit fermentation broth according to the present invention comprises a washing step of washing vegetables / fruits; Heating the washed vegetable / fruit at 100 DEG C for 20 seconds to sterilize the bacteria and coliform bacteria contained in the raw material; A cooling step of cooling the heat-sterilized vegetable / fruit; A step of inoculating lactic acid bacteria to prevent the destruction of the polyphenol component by inoculating the cooled vegetable / fruit with the lactic acid bacteria; Fermented milk prepared by fermenting the fermented milk prepared by fermenting the fermented milk prepared through the low temperature aging step in which the above-mentioned lactobacillus-inoculated vegetable / fruit is aged at 25 ° C. for 7 days; mixing the fermented milk with water and gelatin, Followed by cooling.

상기와 같이 이루어지는 본 발명은 야채/과일 가공시 파괴되는 폴리페놀성분을 유산균(Lactobacillus plantarum )으로 발효하여 폴리페놀성분의 파괴를 억제하며, 발효유 제조에 접목함으로써 현대인에게 부족한 미네랄, 비타민과 함께 폴리페놀이 다량함유되어 있는 효과가 있다.The polyphenols of this invention are vegetables / fruit destroyed during processing, such as made of the lactic acid bacteria (Lactobacillus plantarum ) to inhibit the breakdown of polyphenol component, and by combining with the production of fermented milk, modern people are rich in minerals, vitamins and polyphenols in a large amount.

도 1은 본 발명에 따른 야채/과일 발효액의 공정도.
도 2는 본 발명에 따른 야채/과일 발효액이 첨가된 발효유의 제조공정도.
도 3은 본 발명에 따른 야채/과일 발효액이 첨가된 발효유의 pH 변화.
도 4는 본 발명에 따른 야채/과일 발효액이 첨가된 발효유의 산도 변화.
도 5는 본 발명에 따른 야채/과일 발효액이 첨가된 발효유를 이용한 발효푸딩의 제조공정도.
1 is a process diagram of a vegetable / fruit fermentation broth according to the present invention.
FIG. 2 is a view showing a production process of fermented milk to which a vegetable / fruit fermentation broth according to the present invention is added.
FIG. 3 is a graph showing changes in pH of fermented milk to which a vegetable / fruit fermentation broth according to the present invention is added.
FIG. 4 shows changes in acidity of fermented milk to which a vegetable / fruit fermentation broth was added according to the present invention.
FIG. 5 is a process for producing a fermented pudding using fermented milk to which a vegetable / fruit fermentation liquid is added according to the present invention.

이하, 첨부된 도면을 참조하면서 본 발명에 따른 야채/과일 발효액 제조방법과 상기 발효액을 이용한 발효유 및 발효푸딩의 제조방법에 대하여 설명하도록 한다. Hereinafter, a method for producing a vegetable / fruit fermentation broth according to the present invention and a fermented milk using the fermentation broth and a method for producing the fermented pudding will be described with reference to the accompanying drawings.

현대인들은 스트레스나 각종 전자파, 환경적 요인의 유해로 젊은 나이에도 여러 가지 증세를 느끼는 경우가 늘어나고 있으며, 스트레스를 받으면 스트레스 호르몬이 분비되며 이 호르몬은 세포들을 과잉 자극하여 세포들을 죽게 하는 역할을 한다. 그래서 스트레스가 극심해지면 각종 질병의 원인이 되며 현대인의 안락한 삶을 위해서는 스트레스는 해소하는 것이 중요하다.Modern people are experiencing various symptoms at a young age due to stress, various electromagnetic waves and environmental factors. When stressed, stress hormones are secreted and this hormone stimulates cells to kill cells. Therefore, when stress becomes extreme, it causes various diseases and it is important to relieve the stress for the comfortable life of the modern man.

따라서 본 발명에서는 스트레스 해소용 소재로는 우선적으로 항산화 물질 중 폴리페놀이 많이 함유되어 있는 양배추, 토마토, 브로콜리 및 당근과 같은 4종의 야채와, 복분자, 사과, 배 및 레몬과 같은 4종의 과일을 선정하였다. Therefore, in the present invention, the stress-relieving material is preferably selected from four kinds of vegetables such as cabbage, tomato, broccoli and carrot which contain a lot of polyphenols among antioxidants and four kinds of fruits such as bokbunja, apple, Respectively.

야채/과일을 발효유에 적용하기 위해서는 최종 발효유제품의 미생물적 안전성이 확보되어야 하기 때문에 야채/과일 발효액을 제조하여 영양소의 파괴를 억제하고 항산화물질(폴리페놀)의 함량은 늘릴 수 있는 발효 기술을 적용하여 야채/과일 발효액을 제조하였다.In order to apply vegetable / fruit to fermented milk, microorganism safety of final fermented milk product must be ensured. Therefore, fermentation technology which can increase the content of antioxidant substance (polyphenol) is applied by suppressing destruction of nutrients by manufacturing fermented vegetables and fruits To prepare a vegetable / fruit fermentation broth.

야채/과일의 가열시간에 따른 미생물 안전성을 분석하였다. 가열온도는 모든 미생물이 사멸하는 100℃로 하였으며, 이때 온도 측정 방법은 내부온도가 100℃에 도달한 순간부터 가열시간에 따른 생균수 및 대장균군을 측정하였다. The microbial safety of vegetables / fruits was analyzed by heating time. The heating temperature was 100 ° C. at which all microorganisms died. At this time, the viable cell count and the coliform group were measured according to the heating time from the time when the internal temperature reached 100 ° C.

가열시간에 다른 미생물 변화는 모든 실험구에서의 세균수는 104 cfu/g 이하, 대장균군은 브로컬리와 토마토에서만 검출되었으나, 가열 10초 후에 모든 실험구에서 세균수 10 cfu/g 이하, 대장균군 불검출, 20초 및 30초에서는 세균수 및 대장균군이 검출되지 않았다. Bacterial counts were lower than 10 4 cfu / g in all experimental groups, but only in Broccoli and tomatoes in 10 sec after heating. Bacterial counts and coliforms were not detected at the non-detected, 20 and 30 seconds.

실험구Experimental Section 가열시간(초)Heating time (sec) 00 1010 2020 3030 세균수Number of bacteria 대장균군Coliform group 세균수Number of bacteria 대장균군Coliform group 세균수Number of bacteria 대장균군Coliform group 세균수Number of bacteria 대장균군Coliform group 양배추cabbage 2.2x102 2.2x10 2 불검출Non-detection < 10<10 불검출Non-detection 00 불검출Non-detection 00 불검출Non-detection 토마토tomato 2.4x101 2.4x10 1 불검출Non-detection < 10<10 불검출Non-detection 00 불검출Non-detection 00 불검출Non-detection 브로컬리Broccoli 1.4x104 1.4x10 4 검출detection < 10<10 불검출Non-detection 00 불검출Non-detection 00 불검출Non-detection 당근carrot 3.8x104 3.8x10 4 검출detection < 10<10 불검출Non-detection 00 불검출Non-detection 00 불검출Non-detection 복분자Bokbunja 2.1x101 2.1x10 1 불검출Non-detection < 10<10 불검출Non-detection 00 불검출Non-detection 00 불검출Non-detection 사과Apple 3.2x101 3.2x10 1 불검출Non-detection < 10<10 불검출Non-detection 00 불검출Non-detection 00 불검출Non-detection ship 4.0x101 4.0x10 1 불검출Non-detection < 10<10 불검출Non-detection 00 불검출Non-detection 00 불검출Non-detection 레몬lemon < 10<10 불검출Non-detection 00 불검출Non-detection 00 불검출Non-detection 00 불검출Non-detection

표 1은 가열시간에 따른 미생물 분석을 나타내는 것으로, 양배추, 토마토, 브로콜리 및 당근의 각각의 폴리페놀 함량은 0.154, 0.218, 0.356 및 0.105 mg/g였고, 복분자, 사과, 배 및 레몬의 폴리페놀 함량은 3.883, 0.551, 0.251 및 0.831 mg/g 이었다. 가열시간이 증가할수록 양배추, 토마토, 브로콜리, 사과, 배 및 레몬에서 폴리페놀 함량은 감소하였으며, 10초에는 1~23%, 20초에서는 5~29%, 30초에서는 6~24% 감소하였다. 그러나, 토마토는 가열시간 30초에 약간 증가하는 경향을 보였다. 당근 및 복분자는 가열시간이 증가할수록 폴리페놀 함량이 증가하였으며, 10초에는 1% 및 27%, 20초에서는 3% 및 45%, 30초에서는 5% 및 52% 증가하였다.Table 1 shows the analysis of microorganisms according to the heating time. The polyphenols contents of cabbage, tomato, broccoli and carrot were 0.154, 0.218, 0.356 and 0.105 mg / g, respectively, and polyphenol contents of bokbunja, apple, Were 3.883, 0.551, 0.251 and 0.831 mg / g. As the heating time increased, the content of polyphenols decreased in cabbage, tomato, broccoli, apple, pear and lemon, decreased by 1 ~ 23% at 10 seconds, 5 ~ 29% at 20 seconds and 6 ~ 24% at 30 seconds. However, the tomato showed a slight increase in the heating time of 30 seconds. As the heating time increased, the content of polyphenols increased by 1% and 27% at 10 seconds, 3% and 45% at 20 seconds, and 5% and 52% at 30 seconds, respectively.

(단위:㎎/g)(Unit: mg / g)
가열시간(초)Heating time (sec)
00 1010 2020 3030 양배추cabbage 0.1540.154 0.1190.119 0.1190.119 0.1180.118 토마토tomato 0.2180.218 0.1800.180 0.1550.155 0.1670.167 브로콜리broccoli 0.3560.356 0.3520.352 0.3360.336 0.3340.334 당근carrot 0.1050.105 0.1060.106 0.1080.108 0.1110.111 복분자Bokbunja 3.8833.883 4.9454.945 5.6115.611 5.8895.889 사과Apple 0.5510.551 0.4760.476 0.4680.468 0.4550.455 ship 0.2510.251 0.2440.244 0.2310.231 0.2270.227 레몬lemon 0.8310.831 0.8490.849 0.7700.770 0.7620.762

표 2는 가열시간에 따른 폴리페놀함량의 변화를 나타내는 것으로, 양배추, 토마토, 브로콜리 및 당근의 각각의 지용성 비타민 함량(비타민 A, 비타민 D, 비타민 E)은 양배추 1, 0, 0.1 mg/100g 및 0.078 mg/100g, 토마토 90, 0, 0.6 및 0.004 mg/100g, 브로콜리 128, 0, 2.5 및 0.016 mg/100g, 당근 1270, 0, 0.3 및 0.003 mg/100g 이었고 가열살균처리시 양배추는 1, 0, 0.1 mg/100g 및 0.076 mg/100g, 토마토 159, 0, 0.6 및 0.003 mg/100g, 브로콜리 130, 0, 1.7 및 0.015 mg/100g, 당근 1120, 0, 1 및 0.014 mg/100g으로 나타났으며, 비타민 K의 함량이 소폭 증가하는 것으로 나타났다. Table 2 shows the change in polyphenol content with heating time. The fat-soluble vitamin contents (vitamin A, vitamin D, vitamin E) of cabbage, tomato, broccoli and carrots were 1, 0, 0.1 mg / 0, 0.3, and 0.003 mg / 100 g, respectively. The heat-sterilized cabbage was treated with 1, 0, 0, 0 and 0 mg / , 0.1 mg / 100 g and 0.076 mg / 100 g, tomatoes 159, 0, 0.6 and 0.003 mg / 100 g, broccoli 130, 0, 1.7 and 0.015 mg / 100 g, carrots 1120, 0, 1 and 0.014 mg / , Vitamin K content was slightly increased.

식품명Food name 상태condition 가식부 100g당 함량Content per 100g edible portion 비타민A
(RE)
Vitamin A
(RE)
비타민D
(㎍)
Vitamin D
(㎍)
비타민E
(㎎)
Vitamin E
(Mg)
비타민K
(㎍)
Vitamin K
(㎍)
양배추cabbage 생것raw stuff 1One -- 0.10.1 7878 삶은 것boiled 1One 00 0.10.1 7676 토마토tomato 생것raw stuff 9090 -- 0.60.6 44 삶은 것boiled 159159 -- 0.60.6 33 브로콜리broccoli 생것raw stuff 128128 00 2.52.5 160160 삶은 것boiled 130130 00 1.71.7 150150 당근carrot 생것raw stuff 12701270 00 0.30.3 33 삶은 것boiled 11201120 -- 1One 1414

표 3은 채소 내 지용성 비타민 함량으로서, 양배추, 토마토, 브로콜리 및 당근의 각각의 수용성 비타민 함량(비타민 B1, 비타민 B2, 나이아신, 비타민 C, 비타민 B6, 판토텐산, 비타민 B12, 엽산)은 양배추 0.04, 0.03, 0.3, 36, 0.1, 0.22, 0 및 0.1248 mg/100g, 토마토 0.04, 0.01, 0.6, 11, 0.07, 0.17, 0 및 0.0472 mg/100g, 브로콜리 0.12, 0.26, 1.1, 98, 0.27, 1.12, 0 및 0.0210 mg/100g, 당근 0.06, 0.05, 0.8, 8, 0.07, 0.4, 0 및 28.8 mg/100g 이었고 가열살균처리시 양배추는 0.04, 0.04, 0.1, 26, 0.05, 0.11, 0 및 0.048 mg/100g, 토마토 0.08, 0.03, 0.4, 7, 0.08, 0.13, 0 및 13 mg/100g, 브로콜리 0.06, 0.09, 0.4, 54, 0.12, 0.78, 0 및 0.120 mg/100g, 당근 0.05, 0.05, 0.8, 7, 0.15, 0.23, 0 및 0.014 mg/100g으로 나타났으며, 비타민 C, 판토텐산 엽산항목에서 10~61.5%의 감소량을 보였다. Table 3 shows the contents of water soluble vitamins (vitamin B1, vitamin B2, niacin, vitamin C, vitamin B6, pantothenic acid, vitamin B12 and folic acid) of cabbage, tomato, broccoli and carrot in cabbage 0.04 and 0.03 0.10, 0.1248 mg / 100g, tomato 0.04, 0.01, 0.6, 11, 0.07, 0.17, 0 and 0.0472 mg / 100g, broccoli 0.12, 0.26, 1.1, 98, 0.27, 1.12, 0 And 0.028 mg / 100 g of carrot, 0.06, 0.05, 0.8, 8, 0.07, 0.4, 0 and 28.8 mg / 100g of carrots and the cabbage was 0.04, 0.04, 0.1, 26, 0.05, 0.11, 0 and 0.048 mg / , Tomato 0.08, 0.03, 0.4, 7, 0.08, 0.13, 0 and 13 mg / 100g, broccoli 0.06, 0.09, 0.4, 54, 0.12, 0.78, 0 and 0.120 mg / 100g, carrots 0.05, 0.05, 0.8, 0.15, 0.23, 0 and 0.014 mg / 100g, respectively. The vitamin C and pantothenic acid folate were decreased by 10 ~ 61.5%.

식품명Food name 상태condition 가식부 100g당 함량Content per 100g edible portion 비타민B1
(㎎)
Vitamin B1
(Mg)
비타민B2
(㎎)
Vitamin B2
(Mg)
나이나신
(㎎)
Nashin
(Mg)
비타민C
(㎎)
Vitamin C
(Mg)
비타민B6
(㎎)
Vitamin B6
(Mg)
판토펜산
(㎎)
Pantophenic acid
(Mg)
비타민B12
(㎍)
Vitamin B12
(㎍)
엽산
(㎍)
Folic acid
(㎍)
양배추cabbage 생것raw stuff 0.040.04 0.030.03 0.30.3 3636 0.10.1 0.220.22 00 124.8124.8 삶은 것boiled 0.040.04 0.040.04 0.10.1 2626 0.050.05 0.110.11 00 4848 토마토tomato 생것raw stuff 0.040.04 0.010.01 0.60.6 1111 0.070.07 0.170.17 00 47.247.2 삶은 것boiled 0.080.08 0.030.03 0.40.4 77 0.080.08 0.130.13 00 1313 브로콜리broccoli 생것raw stuff 0.120.12 0.260.26 1.11.1 8787 0.270.27 1.121.12 00 210210 삶은 것boiled 0.060.06 0.090.09 0.40.4 5454 0.120.12 0.780.78 00 120120 당근carrot 생것raw stuff 0.060.06 0.050.05 0.80.8 88 0.070.07 0.40.4 00 28.828.8 삶은 것boiled 0.050.05 0.050.05 0.80.8 77 0.150.15 0.230.23 00 1414

표 4는 채소 내 수용성 비타민 함량으로서, 레몬, 배, 복분자 및 사과의 각각의 지용성 비타민 함량(비타민 A, 비타민 D, 비타민 E)은 레몬 0, 0, 1.6 및 0 mg/100g, 배 0, 0, 0 및 0 mg/100g, 복분자 2, 0, 0 및 0 mg/100g, 사과 3, 0, 1 및 0 mg/100g 이었고 가열살균처리시 레몬 0, 0, 0.1 및 0mg/100g, 배 0, 0, 0 및 0 mg/100g, 복분자 0, 0, 0 및 0 mg/100g, 사과 1, 0, 및 0 mg/100g으로 나타났다.Table 4 shows the content of water soluble vitamin in vegetables. The contents of fat soluble vitamins (vitamin A, vitamin D and vitamin E) of lemon, pear, bokbunja and apple were 0, 0, 1.6, 0 mg / 100g, 0, 0 and 0 mg / 100g, bokbunja 2, 0, 0 and 0 mg / 100g, apples 3, 0, 1 and 0 mg / 0, 0 and 0 mg / 100 g, brambles 0, 0, 0 and 0 mg / 100 g, apples 1, 0 and 0 mg / 100 g.

식품명Food name 상태condition 가식부 100g당 함량Content per 100g edible portion 비타민A
(RE)
Vitamin A
(RE)
비타민D
(㎍)
Vitamin D
(㎍)
비타민E
(㎍)
Vitamin E
(㎍)
비타민K
(㎍)
Vitamin K
(㎍)
레몬lemon 생것raw stuff 00 00 1.61.6 00 열처리Heat treatment 00 00 0.10.1 00 ship 생것raw stuff 00 -- -- -- 열처리Heat treatment 00 -- -- -- 복분자Bokbunja 생것raw stuff 22 -- -- -- 열처리Heat treatment -- -- -- -- 사과Apple 생것raw stuff 33 00 1One -- 열처리Heat treatment 00 -- -- --

표 5는 과일 내 지용성 비타민 함량으로서, 레몬, 배, 복분자 및 사과의 각각의 수용성 비타민 함량(비타민 B1, 비타민 B2, 나이아신, 비타민 C, 비타민 B6, 판토텐산, 비타민 B12, 엽산)은 레몬 0.05, 0.02, 0.7, 70, 0.08, 0.39, 0 및 31 mg/100g, 배 0.02, 0.01, 0.1, 4, 0, 0, 0 및 0 mg/100g, 복분자 0.10, 0.07, 0.6, 0, 0, 0, 0 및 0 mg/100g, 사과 0.01, 0.01, 0.1, 4, 0.06, 0, 0 및 1 mg/100g 이었고 가열살균처리시 모든 처리구에서 검출되지 않았다.Table 5 shows the fat-soluble vitamin content in fruits. The water-soluble vitamin contents (vitamin B1, vitamin B2, niacin, vitamin C, vitamin B6, pantothenic acid, vitamin B12 and folic acid) of lemon, , 0.7, 70, 0.08, 0.39, 0 and 31 mg / 100 g, times 0.02, 0.01, 0.1, 4, 0, 0, 0 and 0 mg / 100 g, brambles 0.10, 0.07, 0.6, And 0 mg / 100g, apples 0.01, 0.01, 0.1, 4, 0.06, 0, 0 and 1 mg / 100g, respectively.

식품명Food name 상태condition 가식부 100g당 함량Content per 100g edible portion 비타민B1
(㎎)
Vitamin B1
(Mg)
비타민B2
(㎎)
Vitamin B2
(Mg)
나이나신
(㎎)
Nashin
(Mg)
비타민C
(㎎)
Vitamin C
(Mg)
비타민B6
(㎎)
Vitamin B6
(Mg)
판토펜산
(㎎)
Pantophenic acid
(Mg)
비타민B12
(㎍)
Vitamin B12
(㎍)
엽산
(㎍)
Folic acid
(㎍)
레몬lemon 생것raw stuff 0.050.05 0.020.02 0.70.7 7070 0.080.08 0.390.39 00 3131 가열heating 00 00 00 00 00 00 00 00 ship 생것raw stuff 0.020.02 0.010.01 0.10.1 44 -- -- -- -- 가열heating 00 00 00 00 00 00 00 00 복분자Bokbunja 생것raw stuff 0.100.10 0.070.07 0.60.6 trtr -- -- -- -- 가열heating 00 00 00 00 00 00 00 00 사과Apple 생것raw stuff 0.010.01 0.010.01 0.10.1 44 0.060.06 -- 00 1One 가열heating 00 00 00 00 00 00 00 00

저장기간(day)Storage period (day) 00 33 55 77 99 폴리페놀
(㎎/100g)
Polyphenol
(Mg / 100g)
4444 5151 5454 5656 5353
미생물변화
(CFC/㎖)
Microbial change
(CFC / ml)
2.18x108 2.18x10 8 4.30x108 4.30x10 8 6.10x108 6.10x10 8 7.20x108 7.20x10 8 7.60x108 7.60x10 8

표 6은 과일 내 수용성 비타민 함량이고, 표 7은 발효액의 폴리페놀 함량의 변화를 나타내는 것으로, 발효액 제조를 위해서 양배추, 토마토, 브로콜리, 당근, 복분자, 사과, 배 및 레몬의 배합 비율은 중량비로 동일한 양으로 설정하였다. 이때의 폴리페놀 함량은 44 mg/100g 이었고 1차 가열(100℃에서 20초) 후 냉각한 다음 유산균(Lactobacillus plantarum)을 1% (1.0 x 108 CFU/mL) 접종한 다음 일주일간 저온 숙성을 한 후 폴리페놀을 측정한 결과 7일 56 mg/100g으로 증가하였다가 감소하여 야채/과일 발효기간은 7일로 하였다.Table 6 shows the content of water-soluble vitamins in fruits. Table 7 shows changes in polyphenol content of the fermentation broth. In order to prepare the fermentation broth, the blending ratios of cabbage, tomato, broccoli, carrot, Respectively. The polyphenol content of the 44 mg / 100g was first heated and then cooled (at 100 ℃ 20 seconds), and then lactic acid bacteria (Lactobacillus plantarum) to 1% (1.0 x 10 8 CFU / mL) inoculation and then for one week after the low-temperature aging and a result of measuring a polyphenol reduced was increased to 7 days 56 mg / 100g vegetable / fruit fermentation period was 7 days Respectively.

<실시예><Examples>

양배추와, 토마토와, 브로콜리 및 당근으로 이루어지는 야채와, 복분자와, 사과와, 배 및 레몬으로 이루어지는 과일을 세척한 후 세절하였다. 이때, 8가지 과일/야채는 모두 동일한 양으로 혼합하였으며, 사과와, 배 및 레몬은 껍질을 벗겨 혼합하였다.Cabbage, vegetables consisting of tomatoes, broccoli and carrots, bokbunja, apples, pears and lemons. At this time, all eight fruits / vegetables were mixed in equal amounts, and apple, pear and lemon were peeled and mixed.

상기 혼합된 야채/과일에 함유된 세균, 대장균군을 살균하기 위하여 100℃로 20초간 가열하였다. The bacteria and coliform bacteria contained in the mixed vegetables / fruits were heated at 100 占 폚 for 20 seconds to sterilize them.

다음으로, 상기 가열살균된 야채/과일을 냉각시킨 다음, 폴리페놀성분의 파괴를 방지할 수 있도록 상기 냉각된 야채/과일에 Lactobacillus plantarum을 1%(1.0x108CFU/mL) 접종한 다음 25℃로 7일간 숙성시켜 본 발명에 따른 야채/과일 발효액을 조성하였다.
Next, after the heat-sterilized vegetables / fruits are cooled, the cooled vegetables / fruits are added with Lactobacillus plantarum was inoculated at 1% (1.0 × 10 8 CFU / mL) and aged at 25 ° C. for 7 days to form a vegetable / fruit fermentation broth according to the present invention.

다음으로, 본 발명에 따라 제조된 야채/과일 발효액을 원유에 첨가하고 발효시켜 발효유를 제조하는 야채/과일 발효액을 이용한 발효유의 제조방법에 대하여 설명하도록 한다. Next, a method of producing fermented milk using a vegetable / fruit fermentation broth to produce fermented milk by adding the fermented broth of vegetable / fruit according to the present invention to crude oil and fermenting the fermented milk will be described.

본 발명은 야채/과일을 발효유에 적용하기 위해서는 최종 발효유제품의 미생물적 안전성이 확보되어야 하기 때문에 야채/과일 발효액 제조하여 영양소의 파괴를 억제하고 항산화물질(폴리페놀)의 함량은 늘릴 수 있는 발효 기술을 적용하여 야채/과일 발효액을 제조하였고 이때의 식이섬유 함량은 0.026g/mL(2.6%)이었다. In order to apply vegetable / fruit to fermented milk, microbial safety of the final fermented milk product must be ensured. Therefore, fermentation technology that can suppress the destruction of nutrients and increase the content of antioxidant material (polyphenol) Was used to prepare vegetable / fruit fermented broth. The content of dietary fiber was 0.026 g / mL (2.6%).

야채/과일 발효액의 농도에 따른 발효유의 품질특성을 분석하였다. 야채/과일 발효액의 첨가 농도는 1, 3 및 5% 첨가시 유산균 스타터의 증식은 첨가 농도 3%까지는 증가하였다가 일정하게 유지되었다. The quality characteristics of fermented milk according to the concentration of vegetable / fruit fermentation broth were analyzed. When 1, 3 and 5% addition of vegetable / fruit fermentation broth was added, the growth of lactic acid bacteria starter increased to 3% concentration and remained constant.

첨가농도(%)Addition concentration (%) 유산균수(CFU/mL)Number of lactic acid bacteria (CFU / mL) 비고Remarks 00 3.30x108 3.30x10 8 24시간 배양후 측정 결과Measurement results after incubation for 24 hours 1One 9.15x108 9.15x10 8 33 6.10x109 6.10x10 9 55 5.40x109 5.40x10 9

표 8은 야채/과일 발효액이 첨가된 발효유의 유산균 수를 나타내는 것으로, 야채/과일 발효액 첨가에 따른 pH 및 산도의 변화에 있어서 모든 실험구의 pH는 발효시간이 증가할수록 감소하였으며, 3 및 5% 첨가구에 따른 차이는 나타나지 않았다. 산도에 있어도 발효시간이 증가할수록 산도는 증가하였으며, 3 및 5% 첨가구에 따른 차이는 나타나지 않았다. 야채/과일 발효액 첨가에 따른 유산균의 증식, 발효시간에 따른 pH 및 산도의 변화에 있어서 일정하였으며 발효유 적용이 가능한 것으로 보인다.Table 8 shows the number of lactic acid bacteria in fermented milk added with vegetable / fruit fermented milk. PH and acidity of fermented milk decreased with increasing fermentation time. There were no differences according to households. The acidity increased with increasing fermentation time in acidity, but no difference was observed between 3 and 5%. The addition of vegetable / fruit fermentation broth showed constant growth of lactic acid bacteria, changes in pH and acidity according to fermentation time, and fermented milk application seems to be possible.

저장기간 및 야채/과일 발효액에 따른 발효유의 식이섬유 변화량은 저장기간에 따른 영향을 받지 않는 것으로 나타났다.The changes of dietary fiber of fermented milk according to storage period and vegetable / fruit fermented liquid were not affected by storage period.

(g/mL)(g / mL) 첨가농도(%)Addition concentration (%) 저장기간(day)Storage period (day) 00 33 55 77 99 00 0.00260.0026 0.00260.0026 0.00280.0028 0.00300.0030 0.00310.0031 1One 0.00290.0029 0.00320.0032 0.00370.0037 0.00400.0040 0.00410.0041 33 0.00350.0035 0.00370.0037 0.00390.0039 0.00420.0042 0.00420.0042 55 0.00440.0044 0.00470.0047 0.00500.0050 0.00530.0053 0.00560.0056

표 9는 야채/과일 발효액 첨가 발효유 식이섬유 변화로서, 야채/과일 발효액 첨가 농도에 따른 관능검사(5점 척도) 결과 야채의 향이 강하여 향 및 맛에 있어서는 첨가 농도가 증가할수록 낮은 경향을 나타내었으며, 3%의 처리구가 향, 맛 및 전체적인 기호도에서 좋은 점수를 얻었다. Table 9 shows the changes in the dietary fiber content of the fermented milk added with the vegetable / fruit fermentation liquid. As a result of the sensory evaluation (5-point scale) according to the concentration of the vegetable / fruit fermentation liquid, 3% of the treatments had good scores in flavor, taste and overall acceptability.

첨가농도(%)Addition concentration (%) incense color flavor 전체적인기호도Overall likelihood 00 4.3±0.384.3 ± 0.38 3.3±0.923.3 ± 0.92 3.7±0.773.7 ± 0.77 3.8±0.463.8 ± 0.46 1One 3.8±0.653.8 ± 0.65 3.6±0.333.6 ± 0.33 3.9±0.253.9 ± 0.25 4.0±0.614.0 ± 0.61 33 3.7±0.513.7 ± 0.51 4.1±0.174.1 ± 0.17 4.2±0.644.2 ± 0.64 4.0±0.614.0 ± 0.61 55 3.3±0.943.3 ± 0.94 4.5±0.374.5 ± 0.37 3.1±0.183.1 ± 0.18 3.5±0.223.5 ± 0.22

표 10은 야채/과일 발효액 첨가 발효유의 관능검사를 나타내는 것으로, 야채/과일 발효액을 이용한 발효유의 제조공정은 다음과 같다. 스타터는 크리스찬한센의 YF-L812(한국구입처 : 주피터 인터네셔널)를 이용하였고, 이때의 균주 구성은 Lactobacillus acidphilus , Bifidobacterium bifidus , Lactobacillus thermophilus로 구성되어있고, 제조 공정은 도 4와 같다.
Table 10 shows the sensory evaluation of the fermented milk containing the vegetable / fruit fermentation liquid. The production process of the fermented milk using the vegetable / fruit fermentation liquid is as follows. The starter used was Christian Hansen's YF-L812 (purchased from Jupiter International, Korea), and the strain was Lactobacillus acidphilus , Bifidobacterium bifidus , and Lactobacillus thermophilus . The manufacturing process is shown in Fig.

<실시예><Examples>

산도 0.16%, pH 6.8의 원유를 90℃로 10분 동안 살균한 다음 원유 100중량부에 대하여 야채/과일 발효액 3중량부를 첨가한 후 제조용 농축냉동스타터(0.0004%)를 접종하였다.Crude oil having an acidity of 0.16% and pH of 6.8 was sterilized at 90 ° C for 10 minutes. Then, 3 parts by weight of the vegetable / fruit fermentation broth was added to 100 parts by weight of crude oil, and then concentrated concentrated freezing starter (0.0004%) was inoculated.

상기 야채/과일 발효액은 양배추와, 토마토와, 브로콜리 및 당근으로 이루어지는 야채와, 복분자와, 사과와, 배 및 레몬으로 이루어지는 과일을 세척하고 세절한 다음 혼합하였다. 이때, 상기 8가지 과일/야채는 모두 동일한 양으로 혼합하였으며, 사과와, 배 및 레몬은 껍질을 벗겨 혼합하였다.The vegetable / fruit fermentation broth was prepared by washing the cabbage, the vegetables consisting of tomato, broccoli and carrot, the bokbunja, the apple, the pear and the lemon. At this time, all of the eight fruits / vegetables were mixed in the same amount, and the apple, the pear and the lemon were peeled and mixed.

상기 혼합된 야채/과일에 함유된 세균, 대장균군을 살균하기 위하여 100℃로 20초간 가열한 다음 냉각시킨 후 폴리페놀성분의 파괴를 방지할 수 있도록 상기 냉각된 야채/과일에 Lactobacillus plantarum을 1%(1.0x108CFU/mL) 접종한 다음 25℃로 7일간 숙성시켰다. To sterilize bacteria and coliform bacteria contained in the mixed vegetables / fruits, the mixture was heated at 100 ° C for 20 seconds and then cooled. Then, the cooled vegetables / fruits were added with Lactobacillus Plantarum was inoculated at 1% (1.0 × 10 8 CFU / mL) and aged at 25 ° C for 7 days.

이후, 43℃로 4~6시간 동안 발효시킨 다음 15℃로 10분 동안 냉각시킨 후 침전된 야채/과일 찌꺼기를 분리할 수 있도록 여과하여 액상의 야채/과일 발효액이 첨가된 발효유를 조성하였다.
Then, the fermented milk was fermented at 43 ° C for 4 to 6 hours and then cooled to 15 ° C for 10 minutes. Then, the precipitated vegetable / fruit residue was filtered to prepare a fermented milk to which a liquid vegetable / fruit fermentation liquid was added.

다음으로, 본 발명에 따라 제조된 발효유를 물과 젤라틴을 혼합한 후 냉각시킨 젤라틴 용액에 첨가한 후 냉각시켜 제조하는 야채/과일 발효액을 이용한 발효푸딩의 제조방법에 대하여 설명하도록 한다. Next, a method of preparing a fermented pudding using a vegetable / fruit fermentation liquid prepared by adding fermented milk prepared according to the present invention to a gelatin solution mixed with water and gelatin, followed by cooling the fermented milk, will be described.

본 발명에 따른 야채/과일 발효액을 이용한 발효푸딩의 제조방법은 50~60℃의 물 60~180중량부에 젤라틴 5~7중량부를 혼합하여 젤라틴 용액을 형성하는 단계와; 상기 젤라틴 용액을 30~35℃로 냉각시키는 단계와; 상기 냉각된 젤라틴 용액 100중량부에 대하여 야채/과일 발효액이 첨가된 발효유 20~140중량부를 첨가하는 단계; 및 상기 발효유가 혼합된 젤라틴 용액을 0~10℃로 1~2시간 동안 냉각시킨 다음 포장하는 단계;를 포함하여 이루어진다.A method for preparing a fermented pudding using a vegetable / fruit fermentation broth according to the present invention comprises: mixing 60 to 180 parts by weight of water at 50 to 60 캜 with 5 to 7 parts by weight of gelatin to form a gelatin solution; Cooling the gelatin solution to 30-35 占 폚; Adding 20 to 140 parts by weight of fermented milk added with vegetable / fruit fermentation broth to 100 parts by weight of the cooled gelatin solution; And cooling the gelatin solution mixed with the fermented milk at 0 to 10 ° C for 1 to 2 hours and packaging.

대조군의 경우는 물 200g, 젤라틴 6g으로 제조하였으며 야채/과일발효액을 이용한 발효푸딩은 물 200g에 각각 10, 30, 50, 70%의 비율로 첨가하여 제조하였다. In the control group, 200 g of water and 6 g of gelatin were prepared, and the fermented puddings using the vegetable / fruit fermentation broth were added to 200 g of water at a ratio of 10, 30, 50 and 70%, respectively.

하기의 표 11은 발효액 푸딩 원료 배합비율을 나타낸 것이다.Table 11 below shows the mixing ratio of the raw materials of the fermentation broth.

(g)(g) controlcontrol 10%10% 30%30% 50%50% 70%70% water 200200 180180 140140 100100 6060 젤라틴gelatin 66 66 66 66 66 발효유Fermented milk 00 2020 6060 100100 140140

야채/과일 발효액이 첨가된 발효유의 첨가 농도에 따른 발효푸딩의 품질특성을 분석하였다. The quality characteristics of fermented pudding were investigated according to the concentration of added vegetable / fruit fermented milk.

먼저, 야채/과일 발효액이 첨가된 발효유의 첨가 농도에 따른 발효푸딩의 수분, 단백질, 지방, 회분 함량을 측정 분석하였다. 수분의 함량은 첨가 농도가 증가할수록 감소 되며 단백질, 지방, 회분은 첨가 농도가 증가할수록 높아지는 경향을 나타내었다. First, moisture, protein, fat and ash content of fermented puddings were measured and analyzed according to the addition concentration of fermented milk added with vegetable / fruit fermented liquid. Moisture content decreased with increasing concentration and protein, fat and ash increased with increasing concentration.

하기의 표 12는 야채/과일 발효액이 첨가된 발효유의 농도에 따른 발효푸딩의 일반성분 변화를 나타낸 것이다.Table 12 below shows changes in the general composition of the fermented pudding according to the concentration of the fermented milk to which the vegetable / fruit fermentation broth was added.

구분division 발효유 푸딩Fermented milk pudding 0%0% 10%10% 30%30% 50%50% 70%70% 수분moisture 82.7382.73 81.2581.25 79.5679.56 75.375.3 72.972.9 단백질protein 4.234.23 5.575.57 5.985.98 6.256.25 6.446.44 지방Fat 0.0010.001 1.011.01 2.312.31 2.852.85 3.653.65 회분Ash 0.010.01 0.10.1 0.190.19 0.320.32 0.390.39

다음으로, 야채/과일 발효액이 첨가된 발효유의 첨가 농도에 따른 발효푸딩의 pH, 산도, 당도를 측정하였다. pH와 산도는 첨가 농도에 따른 변화가 거의 없었으며 당도는 첨가 농도가 증가할수록 높아지는 결과를 나타냈으며 이는 첨가하는 발효유의 영향으로 보인다.Next, the pH, acidity and sugar content of the fermented pudding were measured according to the addition concentration of the fermented milk to which the vegetable / fruit fermentation broth was added. pH and acidity were not changed by addition concentration and sugar content was increased with increasing concentration.

하기의 표 13은 야채/과일 발효액이 첨가된 발효유의 농도에 따른 발효푸딩의 이화학변화를 나타낸 것이다.Table 13 below shows the physicochemical change of the fermented pudding according to the concentration of the fermented milk to which the vegetable / fruit fermentation broth was added.

구분division 발효유 푸딩Fermented milk pudding 0%0% 10%10% 30%30% 50%50% 70%70% pHpH 6.86.8 4.654.65 4.624.62 4.614.61 4.604.60 산도Acidity 0.110.11 0.510.51 0.510.51 0.520.52 0.510.51 당도Sugar content 0.10.1 10.210.2 12.312.3 14.6514.65 17.8917.89

다음으로, 야채/과일 발효액이 첨가된 발효유의 첨가 농도에 따른 발효푸딩의 물성 특성을 분석하였다. 경도, 탄력성, 응집성, 검성, 씹힘성, 점착성 모든 항목에서 발효유 첨가 농도가 증가할수록 낮아지는 경향을 나타냈었다. Next, the properties of fermented puddings were analyzed according to the addition concentration of fermented milk added with vegetable / fruit fermentation broth. Hardness, elasticity, cohesiveness, gumminess, chewiness, and tackiness tend to decrease with increasing concentration of fermented milk in all items.

하가의 표 14는 야채/과일 발효액이 첨가된 발효유의 농도에 따른 발효푸딩의 조직감변화를 나타낸 것이다.Table 14 shows the texture change of fermented pudding according to the concentration of fermented milk added with vegetable / fruit fermented liquid.

구분division 발효유 푸딩Fermented milk pudding 0%0% 10%10% 30%30% 50%50% 70%70% 경도
(hardness)
Hardness
(hardness)
34.2834.28 30.2030.20 24.5024.50 21.5021.50 19.3019.30
탄력성
(springing)
Resilience
(springing)
13.2013.20 12.3012.30 11.9711.97 11.0311.03 10.8010.80
응집성
(cohesiveness)
Coherence
(cohesiveness)
0.520.52 .048.048 0.430.43 0.380.38 0.310.31
검성
(gumminess)
Gum
(gumminess)
26.826.8 26.8226.82 26.2026.20 20.0820.08 20.2120.21
씹힘성
(chewiness)
Chewiness
(chewiness)
3.273.27 3.113.11 3.083.08 2.502.50 2.232.23
점착성
(adhesiveness)
Stickiness
(adhesiveness)
0.710.71 0.650.65 0.610.61 0.550.55 0.450.45

다음으로, 야채/과일 발효액이 첨가된 발효유의 첨가 농도에 따른 발효푸딩의 관능적 특성을 분석하였다. 첨가 농도에 따른 관능검사 결과 첨가농도가 증가할수록 색 및 향은 강하게 나타났으며, 맛에 있어서는 첨가 농도가 증가할수록 높은 점수를 얻었다. 대조구에 비해 야채/과일 발효액이 첨가된 발효유의 첨가구가 전체적인 기호도에 높게 나타났으며, 색, 향 및 맛에 있어서 전반적으로 높게 평가된 50% 처리구를 최종 제품으로 하였다.Next, we analyzed the sensory characteristics of fermented pudding according to the addition concentration of fermented milk added with vegetable / fruit fermentation broth. As a result of the sensory evaluation, the color and aroma were stronger with the addition concentration, and the higher the concentration was, the higher the concentration was. Fermented milk added with vegetable / fruit fermented milk was higher in overall acceptability than the control, and the final product was 50% of the overall highly evaluated color, flavor and taste.

하기의 표 15는 야채/과일 발효액 첨가된 발효유의 농도에 따른 발효푸딩의 관능검사를 나타낸 것이다.Table 15 below shows the sensory evaluation of fermented puddings according to the concentration of the fermented milk added with the vegetable / fruit fermentation broth.

구분division 발효푸딩Fermented pudding 0%0% 10%10% 30%30% 50%50% 70%70% incense 3.8±0.873.8 ± 0.87 4.0±0.234.0 ± 0.23 4.2±0.784.2 ± 0.78 4.5±0.784.5 ± 0.78 4.4±0.124.4 ± 0.12 color 3.8±0.613.8 ± 0.61 4.0±0.784.0 ± 0.78 4.3±0.524.3 ± 0.52 4.5±0.654.5 ± 0.65 4.5±0.894.5 ± 0.89 flavor 3.5±0.523.5 ± 0.52 4.1±0.724.1 ± 0.72 4.1±0.824.1 ± 0.82 4.5±0.234.5 ± 0.23 4.4±0.734.4 ± 0.73 전체적인
기호도
Overall
Likelihood
3.7±0.253.7 ± 0.25 4.0±0.334.0 ± 0.33 4.3±0.574.3 ± 0.57 4.5±0.674.5 ± 0.67 4.4±0.334.4 ± 0.33

이상의 결과를 바탕으로 발명된 제조공정은 다음과 같다. 물 100g, 젤라틴 6g, 야채/과일 발효액이 첨가된 발효유 100g를 첨가한 50% 처리구 제품이 최적의 배합비로 선정하였으며, 이는 야채/과일 발효액이 첨가된 발효유를 푸딩에 접목함으로써 현대인에게 부족한 미네랄, 비타민과 함께 폴리페놀이 다량 함유되어있는 디저트 및 간식식품로 소비자의 기호성과 이용성이 증대될 것으로 기대된다.
Based on the above results, the invented manufacturing process is as follows. 100% of water, 100 g of gelatin and 100 g of fermented milk added with vegetable / fruit fermentation solution were added to the pudding to add the vegetables and fruit fermented milk to the pudding. And dessert and snack foods containing a large amount of polyphenols are expected to increase consumers' palatability and usability.

<실시예><Examples>

양배추와, 토마토와, 브로콜리 및 당근으로 이루어지는 야채와, 복분자와, 사과와, 배 및 레몬으로 이루어지는 과일을 세척하고 세절한 다음 혼합하였다. 이때, 상기 8가지 과일/야채는 모두 동일한 양으로 혼합하였으며, 사과와, 배 및 레몬은 껍질을 벗겨 혼합하였다.Vegetables consisting of cabbage, tomatoes, broccoli and carrots, bokbunja, apples, pears and lemons were washed, cut and mixed. At this time, all of the eight fruits / vegetables were mixed in the same amount, and the apple, the pear and the lemon were peeled and mixed.

상기 혼합된 야채/과일에 함유된 세균, 대장균군을 살균하기 위하여 100℃에서 20초간 가열한 다음 냉각시킨 후 폴리페놀성분의 파괴를 방지할 수 있도록 상기 냉각된 야채/과일에 Lactobacillus plantarum을 1%(1.0x108CFU/mL) 접종한 다음 25℃에서 7일간 숙성시켜 야채/과일 발효액을 조성하였다.In order to sterilize bacteria and coliform bacteria contained in the mixed vegetables / fruits, the mixture was heated at 100 ° C for 20 seconds and then cooled. Then, the cooled vegetables / fruits were treated with Lactobacillus Plantarum was inoculated at 1% (1.0 × 10 8 CFU / mL) and aged at 25 ℃ for 7 days to form a vegetable / fruit fermentation broth.

다음으로, 산도 0.16%, pH 6.8의 원유를 90℃로 10분 동안 살균한 다음 상기 원유 100중량부에 대하여 상기 야채/과일 발효액 3중량부를 첨가한 후 제조용 농축냉동스타터(0.0004%)를 접종하였다. 이후, 43℃로 4~6시간 동안 발효시킨 다음 15℃로 10분 동안 냉각시킨 후 여과시켜 야채/과일 찌꺼기는 제거하고 액상의 야채/과일 발효액이 첨가된 발효유를 조성하였다.Next, the crude oil having an acidity of 0.16% and a pH of 6.8 was sterilized at 90 DEG C for 10 minutes, and then 3 parts by weight of the vegetable / fruit fermentation broth was added to 100 parts by weight of the crude oil, followed by inoculation with concentrated concentrated freezing starter (0.0004% . Then, the fermented milk was fermented at 43 ° C for 4 to 6 hours, cooled at 15 ° C for 10 minutes, filtered to remove vegetable / fruit residue, and fermented with liquid vegetable / fruit fermented milk.

다음으로, 50℃의 물 100중량부에 대하여 젤라틴 6중량부를 혼합하여 젤라틴 용액을 형성한 다음 젤라틴 용액을 30℃로 냉각시켰다. 이후, 상기 냉각된 젤라틴 용액 100중량부에 대하여 야채/과일 발효액 50%가 함유된 발효유 100중량부를 첨가하고, 상기 발효유가 혼합된 젤라틴 용액을 5℃로 1시간 동안 냉각시킨 다음 포장하여 본 발명에 따른 발효푸딩을 조성하였다.Next, 6 parts by weight of gelatin was mixed with 100 parts by weight of water at 50 캜 to form a gelatin solution, and then the gelatin solution was cooled to 30 캜. Then, 100 parts by weight of fermented milk containing 50% of vegetable / fruit fermentation broth was added to 100 parts by weight of the cooled gelatin solution, and the gelatin solution mixed with the fermented milk was cooled at 5 캜 for 1 hour and then packaged, To form a fermented pudding.

Claims (5)

야채/과일 중 두 가지 이상을 선택하여 세절하고 혼합하는 단계;
상기 혼합된 야채/과일을 100℃로 20초간 가열하여 원료 내에 함유된 세균, 대장균군을 살균하는 가열살균단계;
상기 가열살균된 야채/과일을 냉각하는 냉각단계;
상기 냉각된 야채/과일에 유산균주를 접종하여 폴리페놀성분의 파괴를 방지하는 유산균주 접종단계;
상기 유산균주가 접종된 야채/과일을 25℃로 7일간 숙성하는 저온숙성단계;
를 포함하여 이루어지는 것을 특징으로 하는 야채/과일 발효액 제조방법.
Selecting and finishing and mixing two or more of vegetables / fruits;
Heating the mixed vegetable / fruit at 100 DEG C for 20 seconds to sterilize bacteria and coliform bacteria contained in the raw material;
A cooling step of cooling the heat-sterilized vegetable / fruit;
A step of inoculating lactic acid bacteria to prevent the destruction of the polyphenol component by inoculating the cooled vegetable / fruit with the lactic acid bacteria;
A low temperature aging step of aging the vegetables / fruits inoculated with the lactic acid bacteria at 25 占 폚 for 7 days;
The method for producing a vegetable / fruit fermentation broth according to claim 1,
제1항에 있어서, 상기 야채/과일은 양배추, 토마토, 브로콜리, 당근, 복분자, 사과, 배 및 레몬이 동일한 양으로 혼합된 것을 특징으로 하는 야채/과일 발효액 제조방법.The method according to claim 1, wherein the vegetable / fruit is a mixture of cabbage, tomato, broccoli, carrot, brambles, apple, pear and lemon in the same amount. 제1항에 있어서, 상기 유산균주는 Lactobacillus plantarum인 것을 특징으로 하는 야채/과일 발효액 제조방법.The method of claim 1, wherein the lactic acid bacteria are Lactobacillus plantarum . &lt; / RTI &gt; 제1항 내지 제3항 중 어느 한 항의 야채/과일 발효액의 제조방법에 의해 제조된 발효액을 원유에 첨가하고 발효시켜 제조하는 것을 특징으로 하는 야채/과일 발효액을 이용한 발효유의 제조방법.A process for producing fermented milk using the vegetable / fruit fermentation broth, which comprises adding the fermentation broth produced by the method for producing a vegetable / fruit fermentation broth of any one of claims 1 to 3 to crude oil and fermenting the same. 제4항의 제조방법에 따라 제조된 발효유를 물과 젤라틴을 혼합한 후 냉각시킨 젤라틴 용액에 첨가한 후 냉각시켜 제조한 것을 특징으로 하는 야채/과일 발효액을 이용한 발효푸딩의 제조방법.
A process for producing fermented puddings using the vegetable / fruit fermentation broth, which comprises mixing fermented milk prepared according to the process of claim 4 and gelatin solution mixed with water and gelatin, followed by cooling.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190068461A (en) * 2017-12-08 2019-06-18 재단법인 발효미생물산업진흥원 Apple fermented beverage using Weissella confusa SRCM100892 strain and method for producing jelly using the same
CN113678976A (en) * 2021-08-26 2021-11-23 上海应用技术大学 Chinese prickly ash leaf fermented beverage with free radical removing activity and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190068461A (en) * 2017-12-08 2019-06-18 재단법인 발효미생물산업진흥원 Apple fermented beverage using Weissella confusa SRCM100892 strain and method for producing jelly using the same
CN113678976A (en) * 2021-08-26 2021-11-23 上海应用技术大学 Chinese prickly ash leaf fermented beverage with free radical removing activity and preparation method thereof

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