KR20150096357A - Agrochemical composition for controlling scale insect pest comprising Quisqualis indica extract - Google Patents

Abstract

The present invention relates to a pesticide composition for controlling scale insects wherein the composition comprises a polar solvent extract of Quisqualis indica which is safe to humans, animals and environment, and exhibits an excellent effect of controlling the scale insects by spraying or applying the composition to crops during a generation period of the scale insects.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a pesticidal composition for controlling pest insects,

The present invention relates to a pesticide composition for controlling insect pests, and more particularly, to a pesticide composition for insect pest insect control containing a polar solvent extract of a pest insect plant extracted from a mushroom.

Pest bugs are pests that directly or indirectly cause damage to trees, trees, as well as special crops such as fruit trees, flowers, and ginseng. There are more than 7,000 species of pod bugs in the world and 159 species in Korea. Coccoidea contains 20 families and is divided into two groups : Diaspididae , Coccidae , Pseudococcidae , Eriococcidae , Ischeliaceae , Margarodidae ) are common.

Among them, about 2,500 species of pod bugworm are known all over the world. They produce shells with shell-shaped pods in the trees and damage them, and 72 species are recorded in our country.

There are more than 1,200 varieties of wheat beetles worldwide. Some species are causing problems worldwide due to international trade. In Korea, 31 species are known, including Ceroplastes japonicus .

There are about 2,000 species of insect pests in the world, and this species has the characteristic that females wrap their faces. There are 25 known species in Korea including Crisicoccus pini .

There are more than 500 species of pod intervertebrata worldwide , and six species are known in Korea, including Eriococcus lagerstroemiae . There are more than 250 species of Isseeridae in the world and 8 species are known in Korea including Matsucoccus thunbergianae .

The larvae of the pods are most commonly affected by nutrients such as trees, trees, trees and trees, as well as various kinds of flowering and flowering trees. The main species that damage to occur in the country include yiseri Oh pod beolregwa (Margarodidae) Sol peel pod worm (Matsucoccus thunbergianae) belonging to the pocket pod worms pocket pod beolregwa (Eriococcidae) (Eriococcus lagerstroemiae), wheat pod beolregwa (Coccidae) Ceroplastes japonicus belonging to the genus Pseudococcidae, Crisicoccus pini belonging to the Pseudococcidae and Planococcus citri .

Intertwine worms cover the body with various types of wax materials (pods) and fix the body to the bark of leaves or leaves of the tree. Since they live in sucking the sap in them, they do not come into direct contact with the body of the worms It is a pest which has a low effect of controlling the medicine and is difficult to control. As a result of abuse of pesticides due to this, the natural enemies are rapidly decreasing due to the abrupt decrease of natural enemies. Especially, the damage to roadside trees and landscaping trees frequently spraying medicines is more serious. In addition, it is a pest which has a high frequency of detection even in the process of quarantine of imported plants because it inhibits photosynthesis and damages the product by generating secondary soot disease due to nectar, virus-mediated and secretory secretion from juvenile.

The control of pod bugs has mainly used chemical control, but it has caused side effects such as danger to human life, environmental risks such as risks to non - target organisms, residual toxicity and ecosystem disturbance, and appearance of resistant individuals. Therefore, it is required to develop a control agent that can replace chemical pesticides to control insect pests.

In recent years, there has been a growing demand from farmers who demand quality agricultural products and produce high-quality safe agricultural products to create added value. As an alternative method to meet this demand, plant extracts have been used as food materials for a long period of time because they are naturally derived materials and can be effectively used for control of insect pests and insects. In addition, plant extracts may exhibit different effects depending on the kind of plant and target insect pests.

The patents disclosed on the natural plant extracts used as compositions for controlling pests have heretofore been disclosed in Korean Patent No. 10-0971314 as plant essential oils such as bay, caraway seed, cardamon ceylon, lemongrass, lime disc 5F, oregano, Natural control agents have been disclosed for heating pests comprising the resulting compounds. EP 10-0679661 discloses an environmentally friendly mite control agent comprising a plant alcohol extract selected from cloves, cinnamon, mustard, horseradish, and the like. Patent No. 10-0596622 discloses a composition for controlling head lice comprising a plant extract such as warmwood oil, peppermint oil, penny royal oil, and spearmint oil as an effective ingredient. None of the above documents discloses an insect control agent using an insect pest insect control agent or a further insect pest control agent.

Accordingly, it is an object of the present invention to provide a novel natural pesticide composition for environmentally friendly insect pest control that can replace chemical pesticides against interstitial pest insects.

Another object of the present invention is to provide a method for controlling pest insects, which directly or indirectly causes damages to crops using the pesticide.

The above object of the present invention can be achieved by a method for producing a hot water extract, Measuring and evaluating the insecticidal effect after spraying the hot-water extract on a brush bark worm, indoors or outdoors; Extracting methanol with methanol and fractionating the methanol by extraction solvent; Measuring and evaluating the insecticidal effect after spraying the extract of the solvent fraction on mandarin pod worms and pocket worm worms; Measuring and evaluating the insecticidal effect of Spearman hexane fraction sprayed on pine worms at different concentrations; This hexane fraction was obtained by purification and component analysis.

The extraction site of the Shiitake mushroom extract according to the present invention is not particularly limited and is preferably selected from the group consisting of the entire plant selected from Shiitake, or the root, stem, leaf, flesh, flower, fruit, seed and skin of Shiitake It may be a part of the chosen sage.

The extract may be a hot water extract, a hot water extract, a cold water extract, an organic solvent extract, or a sanguinea essential oil of Sagunja, and most preferably a hexane extract of Sagunja.

Preferably, the dried or organic matter of the seedlings is ground or ground, and the whole is ground and mixed with an extraction solvent such as alcohol, alcohol diluted water or an organic solvent, and filtered. And drying the solvent. The alcohol is a lower alcohol having a carbon number of 1 to 4 (C4), preferably ethanol, methanol, propanol or butanol, and most preferably ethanol or methanol. The alcohol diluted water may be used by mixing 20 to 90% by volume of alcohol with water. The organic solvent may be hexane, chloroform, ethyl acetate, butanol, etc., and may be sequentially fractionated according to the polarity of the organic solvent.

The saccharide essential oil may be prepared by conventional extraction, distillation or compression, and may be manufactured or purchased according to the whole or part of the sari, such as root, stem, leaf, flesh, flower, fruit, seed and skin have.

The active ingredient of the extract according to the present invention may be in a concentration range of 0.1 to 100,000 ppm, more preferably in a concentration range of 1 to 10,000 ppm. If the concentration range is lower than the above range, the insect worms can not be completely controlled. If the concentration range is higher than the above range, the economical efficiency is lowered.

The controlling agent of the present invention contains the above-mentioned plant extract, plant essential oil or compound as an active ingredient, and may contain one or more kinds of active ingredients. In addition to the active ingredient, the controlling agent may further comprise a suitable diluent or excipient depending on the formulation and / or purpose of use. The excipient may be a conventional material according to the formulation, and when formulated, a filler, an extender, a wetting agent, a disintegrant, a surfactant, or the like may be used. The diluent or excipient typically used may include water, dextrin, calcium carbonate, lactose, propylene glycol, liquid paraffin, physiological saline and the like, more preferably 0.1% Tween 80 per liter (L) Tea distilled water can be used.

The formulations for controlling agents may be formulated into granules, powders, aerosols, powders, syrups, liquids and solutions, aerosols, EXTRACTS, OINTMENTS, FLUIDEXTRACTS, EMULSIONS, SUSPESIONS, TABLETS, SPIRITS, CAPSULES or Creams CREAMS), and it is preferably used as a suspension agent.

The dosage of the pesticidal composition or the insecticide can be applied according to the target crop, the step of growing the pod bug, the kind of the pod bug, or the like, and preferably is diluted to a concentration of 10 to 10,000 ppm to spray the damaged crop sufficiently And most preferably in a concentration range of 10 to 1,000 ppm.

The present invention has an effect of providing a novel insecticidal composition for controlling insect pest insecticides containing a polar solvent extract, and has an excellent effect that it can be effectively used for pest insect control while being safe for human or animal environments.

FIG. 1 is a photograph showing an outdoor experimental work for the control of a brush shell scouring beetle of Sawny hot-water extract,
FIG. 2 is a photograph showing the methanol extract of Sajungja obtained under reduced pressure for each solvent fraction,
FIG. 3 is a photograph of a laboratory experiment in which a methanol extract of Sajungja is treated on a bamboo pod pod worm,
FIG. 4 is a photograph showing the pod bug worm (left) killed by the hexane layer treatment of the methanol extract of Shiitake mushroom and the pod bug (right)
5 is a graph showing a GC-MS chromatogram of alpha pinene.

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

Example 1: Analysis of insecticidal activity of the hot water extract of Sajungja on the brush bark worm

1-1: Preparation of hot water extract

The seedlings used in the present invention were purchased from herbal medicines, dried for one week in a laboratory in a shade, and then pulverized to a fine powder using a pulverizer (Hanil HMF370, Korea). The pulverized powder was sterilized at 121 ° C and 1.5 atm for 20 minutes using a high-pressure sterilizer (JEIO Tech AC-12, Korea) in a 1,000 mL Erlenmeyer flask, and 40 g of the sample and 400 mL of the first distilled water were placed. After extracting, the solids were filtered using a 150 mm diameter Sanyo filter paper (No 2, Japan), and then the liquid material was stored in a 500 mL cell culture flask and refrigerated.

1-2: Preparation of brush shell pod bug

In the examples of the present invention, the pine bark worms were used and collected from the neck of the pine bark worms in Changseon-myeon, Namhae-gun, Gyeongsangnam-do.

In order to determine the control efficacy of the Salicornia japonica extract prepared from the above-mentioned Salicornia spp., The extracts of Salvia officinalis were sprayed on the salivary gill worms according to the following method.

Experimental Example 1: Analysis of insecticidal activity of hot water extracts of Sawna japonica on the brush bark worms

Indoor activity assays of Sorghum extracts on brush bark worms were carried out two times. The first experiment was carried out using a 500 mL small sprayer for home gardening. Sagunja extract was treated with 10,000ppm concentration of fenitrothion 50% EC 1,000 times in the cold storage after hot water extraction. In the natural occurrence of the pine bark worm, which occurs naturally in the southern sea of Gyeongsangnam-do, the high-density branches of the pine bark worms are cut into 20-30 cm length by pruning shears and brought to the laboratory. I sprayed it down. After shaking, the branches were cut on a newspaper for 30 minutes, cut into 5-7㎝ length, placed in Incu Tissue (65.4x65.4x98.2 ㎜, SPL, Korea) ~ 80%) (JEIO Tech GC-1000 TLH, Korea). The experiment was carried out with 4 repetitions. Thirty days after the treatment, the presence or absence of lethal bark worms was examined under a dissecting microscope (Nikon SMZ-1000, Japan). When the bug worms emerged after emergence, they were determined to be alive. In the case where it is determined that it is dead.

The second experiment was carried out using a Potter spray tower (Burkard Manufacturing Ltd., Rickmansworth, UK). After harvesting the larvae, they were cut into 5-7㎝ lengths. One 90 mm filter paper (Advantec No 2, Japan) was placed on an insect breeding dish (90 x 40 ㎜) . The insect breeding dish was placed in a drug spraying tower and sprayed with 1 ml of 10,000 ppm of Sajunja hot water extract. In the untreated treatment, only 1 mL of sterilized water was treated. The experiment was repeated three times with one repetition of one insect breeding dish. After 14 days, the presence or absence of life was investigated. The results are shown in Table 1 below.

Experimental Example 2: Analysis of outdoor insecticidal activity of the hot water extract of Sa-kunja on the brush bark worm

Experiments were carried out on the nymphs in the naturally occurring stands of brush shell pod worms in Changseon - myeon, Namhae - gun, Kyungsangnam - do, South Korea. The experiment was carried out two times with different timings for the seeds of less than 2 m in height within 5 years.

The first experiment was carried out on February 16, 2012, and 1,000 ppm of hot water extract of Sagunja and 1,000 times of penitrothion (50%) emulsion, which is a comparative agent, were treated. The treatments were labeled with vinyl tape for high density branches of brush shell pod worms, and then sprayed with a home gardening sprayer to the extent that the chemical solution flowed down. The effect was investigated on May 12th. Branches of the treated part were cut using vestibular scissors 20-30cm long, and then put into a zipper pack, and transported to the laboratory, and examined for deaths under a dissecting microscope. The lethal occurrence was determined to be alive after the allegation, and the remaining individual was judged to be alive. The alienated and hardened ones were judged to be dead. The treatments were repeated four times with one branch, and only the water was sprayed without treatment.

The second experiment was carried out on March 26, and the treatment method was the same as the first experiment, and the investigation was conducted on the same date on May 12th. The results are shown in Table 2 below.

As can be seen in [Table 1] and [Table 2], in the concentration range of 1,000 ppm of the present invention, the high extractability rate (pest insect target: 95.9%, outdoor winter treatment: 71.7 %, Outdoor spring treatment: 79.6%).

Therefore, the water extract of Saengunja according to the present invention was effective both indoors and outdoors against the brush bark worm.

Example 2: Analysis of the insecticidal activity of the methanol extract of Sagunja on fractional worms

Based on the results of Example 1, the insecticidal activity of each of the extractants was examined by extracting methanol with methanol.

2-1: Preparation of methanol extract of Sajungja

The methanol extracts of the seedlings used in the present invention were prepared by suspending a dry MeOH extract in distilled water (1000 mL) and performing solvent fractionation while gradually increasing the polarity of the solvent. The suspension was transferred to a separation funnel, 1,000 mL of n-hexane was added, mixed thoroughly and allowed to stand, then the n-hexane layer of the supernatant was separated and this fraction was applied two more times. The n-hexane fraction was concentrated to dryness using a rotary evaporator. This procedure was repeated _{three times} with chloroform (CHCl ₃ , 1,000 mL), ethyl acetate (EtOAc, 1,000 mL) and n-butanol (BuOH, 1,000 mL) three times and each fraction was added to a 1,000 mL round- And then concentrated and dried with a rotary vacuum evaporator (N-1000, Japan). 91.8 ㎎ of the extract was obtained from hexane fraction, and 84 ㎎ of CHCl ₃ fraction, 80.3 ㎎ of EtOAc fraction and 57.2 ㎎ of BuOH fraction, and 89.5 ㎎ fraction of H ₂ O fraction were obtained. Five fractions obtained by solvent fraction were added 1 mL of MeOH per 1 mg, and 1,000 ppm of a black sample was prepared in a glass vial and homogenized with a vortex mixer.

2-2: Preparation of interspecies

The tangerine pod bug beetle used in the present invention was collected in Pyracantha angustifoila (Franch.)) Growing house of the Namhae-gun, Gyeongsangnam-do, South Korea and used in the collection. The pickled beetle was cut with pruning shears And then taken to the laboratory. The first instar larvae immediately after hatching were used.

The pod intertwining worms were collected in July at the Lagerstroemia indica around Gyeongsang University campus in Jinju, Gyeongsangnam - do. The harvesting was carried out by cutting the damaged branches of the insect worms with pruning shears into the zipper pack and bringing them to the laboratory. In addition, Pinus densiflora Siebold & Zuccarini, Gyeongsang National University pearl pine worms were collected in August and used in experiments. The harvesting was carried out by cutting the damaged branches of the insect worms with pruning shears into the zipper pack and bringing them to the laboratory.

In order to determine the control efficacy of the methanol extracts of Shiitake mushroom prepared above, each of the Shiitake mushroom extracts was sprayed on the orange mushroom worms and pod worm worms according to the following method,

Experimental Example 3: Analysis of the insecticidal activities of methanol extracts of Sajungja methanol extracts against the tangerine powdery moth pods and pestle worms

Two pills of 90 mm Advantec filter paper (No 2, Japan) were placed on Insect breeding dish (90 x 40 mm) and 2 mL of distilled water was applied to the piranha tangerine pod worms and bongo tree pod worms collected outdoors Fifteen hatching nymphs were inoculated with a fine brush. Then, each of the insect pests was treated with a 500 mL domestic gardening sprayer at a concentration of 100 ppm for each of the Sajunga extract fractions. In order to investigate the effect of solvent, 100 ppm of MeOH was added to sterilized water. As a control agent, methidathion (40%) emulsion was treated.

Tangerine powdery mildew worms and pocket worm worms were examined on a dissecting microscope for 24, 48 and 72 hours after the treatment. The presence or absence of life or death was stimulated by each brush with a brush. Anything without reaction was deemed dead, and those that were hard or hardly fluid were judged dead. All treatments were performed in 4 replicates. The results are shown in [Table 3] and [Table 4] below.

Therefore, the methanol extract of Shiitake mushroom according to the present invention exhibited activity against intergrowth worms in all the solvent layers, and especially the activity of the hexane layer was high. The activity of the methanol extracts by solvent fraction was 94.9% (Table 3) and 95.1% (Table 4) in the hexane fraction of balsam pod and tangerine pod worms.

Experimental Example 4: Analysis of insecticidal activities of methanol extracts of Sajungja on the pine woody beetle by concentration of hexane fraction

Hexane fractions were treated with 200 ppm and 100 ppm, 50 ppm and 25 ppm, respectively, for the pine flounder, Two 90 - mm filter papers (Advantec No 2, Japan) were placed on an insect breeding dish (90 x 40 mm) and treated with 2 mL of distilled water. Fifteen hatching eggs were inoculated with a fine brush and sprayed at a concentration of 500 mL using a home garden sprayer. Untreated was treated only with sterilized water and one container was repeated 4 times with one iteration. After 24, 48, and 72 hours after treatment, the presence or absence of lethality of the pine weevil hatching larvae was confirmed under a dissecting microscope. The presence or absence of life or death was stimulated by each individual with a brush, and those without reaction were regarded as dead. The results are shown in Table 5 below.

In Table 5, the hexane fraction of the methanol extracts of Sajungja showed insecticidal effect against 73.3% of pine worms at 25 ppm and more than 82.2% at over 50 ppm.

Example 3: Analysis of the insecticidal activity of the fractions of Sajunga hexane against the insect pests

On the basis of the results of Example 2, the hexane fractions of the methanol extract of Sagitta mushroom were expanded in the examples of the present invention to investigate their insecticidal activity.

3-1: Purification of Safflower hexane fraction

In order to purify the hexane fraction used for the present invention, a methanol column (Merck 7734, 200 g) of methanol extract of Sagunja was filled with a glass column (3 cm x 98 cm) and the n-hexane fraction was dissolved in n-hexane column chromatograph.

1024 active fractions obtained by step-wise elution with a solvent system of n-hexane-chloroform (CHCl ₃ ) were eluted with hexane-EtOAc (8: 2) on silica gel TLC (thin layer chromatography) Lt; / RTI > The presence or absence of a coloring spot was observed when a UV-absorbing or coloring spot was formed on the active region on the TLC plate through the UV spectrum, or when H ₂ SO ₄ was sprayed as a dye.

Thus, a total of 10 groups were divided into active fractions, transferred to a 500 mL round-bottomed flask, and concentrated to dryness using a rotary vacuum concentrator. HS6 (60 mg), HS4 (330 mg), HS5 (600 mg), HS6 (190 mg), HS7 (240 mg), and HS7 (50 mg) Ten subfractions of HS8 (70 mg), HS9 (240 mg) and HS10 (350 mg) were obtained.

3-2: Preparation of interspecies

The tangerine powder scarab beetle used in the experiment of the present invention was collected at the same place as that used in Experimental Example 3 and the experiment was conducted.

In order to determine the control efficacy against the insect pests of the above-prepared Sanguinehexane fractions, each of the mushroom extracts was sprayed on the pine worm pod worms in the following manner, and the presence or absence of their survival was determined.

Experimental Example 5: Analysis of the insecticidal activity of the fractions of Saccharomyces hexane fractions against the pine worms

Hexane fractions were separated and purified by silica gel chromatography, and the insecticidal activity against the insect pests of each fraction was examined. 1 mL of MeOH per 1 mg was added to the vial to make a 1000 ppm assay sample and homogenized with a vortex mixer.

All treatments were carried out on insect breeding dish (90 x 40 ㎜) of piranha of the tangerine flounder, 2 sheets of 90 mm filter paper (Advantec No 2, Japan) containing 2 mL of distilled water, Fifteen of them were inoculated and sprayed at a concentration of 100 ppm using a 500 mL domestic gardening atomizer. After 24, 48, and 72 hours of treatment, the presence or absence of lethal hatching larvae was examined under a dissecting microscope, and one vessel was repeated four times with one repetition. The results are shown in Table 6 below.

The 10 subfractions of the hexane layer showed a mortality rate of over 80% for the orange mite worms (Table 6). And each subfraction contains alpha-pinene, eugenol, methyl-palmitate, methyl-myristate, plmitic acid, phthalic acid mono And phthalic acid mono (2-ethylhexyl) ester were isolated. Among them, the half-lethal concentration of insect pests was 0.0114 ppm (Table 6).

Therefore, the insecticidal activity against the insecticidal insecticides of the lower fraction of the hexane layer of the methanol extract of Sajungja was higher in all fractions.

The insecticidal activity of the preparations prepared by further adding a diluent or excipient to the fractions of the herringbone hexane containing the active ingredient of any one of the above compounds showed almost the same effect as the above results. The description is omitted.

Accordingly, it has been confirmed that the composition of the present invention can be usefully used as a pesticide composition capable of biologically controlling an insect pest.

Example 4: Analysis of constituents of saccharide hexane layer

Based on the results of Example 3, the active substance was investigated in the hexane fraction of the methanol extract of Sajungja in the examples of the present invention.

4-1: Analysis of constituents of the saccharide hexane layer

Agilent 7890A Gas chromatography-mass spectrometry (GC-MS) was used to analyze the components of the hexane layer used in the present invention. The column used Agilent HP-5MS capillary and the library used Wiley DB. Detector was full scan using Agilent 5975C, flow rate was He and flow rate was 0.9 ml / min. The temperature of the oven was as shown in Table 7. In the measurement, samples were diluted in hexane for HPLC and injected with 1 μl per sample using auto sampler.

As a result of the experiment, it was found that the eugenol, compound Ⅱ, methyl-palmitate, methyl-myristate (compound Ⅳ), α-pinene (compound Ⅰ) , Plmitic acid (compound V), phthalic acid mono (2-ethylhexyl) ester, and compound VI were isolated.

Experimental Example 6: Analysis of insecticidal activity against intertwined insects of a substance isolated from the fractional layer of Saccharomyces hexane against a pod intertwining worm

In the present invention, α-pinene was treated by pestle worms in order to test the insecticidal activity of α-pinene isolated from all the hexane subfractions of the Sagunja MeOH extract. The test samples were diluted in EtOH to 100 ppm with 98% α-pinene (Sigma-Aldrich, USA) and diluted with distilled water to prepare 10 ppm, 5 ppm, 1 ppm, 0.5 ppm and 0.1 ppm. Two sheets of 90 mm filter paper (Advantec No 2, Japan) were placed on an insect breeding dish (90 x 40 mm) and treated with 1 mL of a test sample. Untreated was treated with a mixture of 100 ppm EtOH and a fine brush Fifteen hatching birds were inoculated. After 24, 48, and 72 hours of treatment, the presence or absence of lethal nematode hatching larvae was examined under a microscope and four replicates were performed in one container.

The results of the experiment are shown in Table 8 below. The half - life mortality for the insect pests of the alpininen was 0.0114 ppm, which was very high. Figure 5 shows the GC-MS chromatogram of the alpha pinene.

Claims (9)

Of a plant of the genus Quisqualis ( Quisqualis indica) Sequential polar solvent extraction fractions of n-hexane, chloroform, ethyl acetate and n-butanol in a suspension of distilled water in alcohol extract. An insecticidal composition for controlling insect pest weeds, characterized in that it contains any one of the polar solvent extract fractions of the plant of claim 1 as an active ingredient. The polar solvent extract fraction of a plant of claim 1, wherein the extract fraction contains at least one of alpha pinene, yugenol, methyl palmitate, methyl myristate, palmitic acid and phthalic acid monoester as an active ingredient, Pesticide composition for controlling. The insecticidal composition according to claim 3, wherein the insecticidal composition for controlling insect pest is comprised of at least one pharmaceutically acceptable diluent or excipient. [Claim 5] The insecticidal composition according to claim 4, wherein the diluent or excipient is at least one selected from the group consisting of water, dextrin, calcium carbonate, lactose, propylene glycol, liquid paraffin, and physiological saline. 6. The composition of claim 5, wherein the composition is selected from the group consisting of GRANULES, LPTIONS, AROMATIC WATERS, POWDERS, SYRUPS, LIQUIDS AND SOLUTIONS, AEROSOLS, EXTRACTS, OINTMENTS, FLUIDEXTRACTS, EMULSIONS, SUSPESIONS, TABLETS, SPIRITS, CAPSULES or CREAMS. The insecticidal composition for controlling insect pest weeds. A method for biological control of an insect pest, characterized in that the extract fraction of claim 1 or the composition of claim 2 is contacted with an insect pest. 8. The method according to claim 7, wherein the contact is sprayed with the extract or the composition or applied to crops to an insect insect generator. The method of claim 7, wherein the pod worm acids are selected from the group consisting of pods beolregwa (Diaspididae), wheat pod beolregwa (Coccidae), powder pod beolregwa (Pseudococcidae), pocket pod beolregwa (Eriococcidae), yiseri Oh pod beolregwa (Margarodidae) . ≪ / RTI >

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