KR20150077594A - Retort pouched oyster soup with complex extract and method for making the same - Google Patents

Retort pouched oyster soup with complex extract and method for making the same Download PDF

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KR20150077594A
KR20150077594A KR1020130166126A KR20130166126A KR20150077594A KR 20150077594 A KR20150077594 A KR 20150077594A KR 1020130166126 A KR1020130166126 A KR 1020130166126A KR 20130166126 A KR20130166126 A KR 20130166126A KR 20150077594 A KR20150077594 A KR 20150077594A
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weight
parts
oyster
retort pouch
extract
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KR1020130166126A
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Korean (ko)
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김선근
이소정
이바다
김의현
강은경
황영숙
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김선근
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L23/00Soups; Sauces; Preparation or treatment thereof
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/90Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W90/00Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
    • Y02W90/10Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics

Abstract

The present invention provides retort pouched oyster soup, which comprises: 2-4 parts by weight of an oyster complex extract comprising a residual enzyme decomposed product concentrated as treating a hot water extract and a residual hot water extract of oyster with a protein hydrolysis enzyme; 15-20 parts by weight of oyster; 60-70 parts by weight of a basic meat broth manufactured by mixing and heating dried anchovy, radish, leek, kelp, refined salt, refined water, and an oyster steaming solution; and 10-15 parts by weight of one or at least one minor ingredient selected from the group consisting of sea weed, radish, soybean sprout for steaming, garlic, and red pepper so as to be manufactured by heating and sterilizing for 8-15 minutes with a Fo value after filling and sealing in a retort pouch.

Description

굴 복합추출물을 이용한 레토르트파우치 굴국 및 그의 제조방법{Retort pouched oyster soup with complex extract and method for making the same}       Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a retort pouched oyster soup,

본 발명은 레토르트파우치 굴국에 관한 것으로서, 더 상세하게는 굴 복합추출물을 이용한 레토르트파우치 굴국 및 그의 제조방법 관한 것이다. BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a retort pouch excrement, and more particularly, to a retort pouch excrement using an oyster complex extract and a manufacturing method thereof.

우리나라에서 굴(Crassostrea gigas)은 1960년부터 경남지역을 중심으로 연승수하식 방법이 널리 보급되면서 생산량이 증가하였다. 특히 1972년 미국 식품의약품안전청(FDA)과 한미패류위생협정을 체결하면서 우리나라의 청정해역에서 생산되는 굴의 우수성을 세계적으로 인정받았으며, 산업자원부는 2001년 수산물로는 처음 냉동굴을 세계 일류상품으로 선정한 바 있다. In Korea, production of Crassostrea gigas has been increasing since 1960, when the method of long lineage gathering has spread widely in Gyeongnam area. In particular, in 1972, the US and Korea Food and Drug Administration (FDA) signed a sanitation agreement with the United States of America. In 2001, the Ministry of Commerce, Industry and Energy (MOCIE) Have chosen.

현재 우리나라의 연간 양식산 굴 생산량은 각부(殼附) 굴 기준으로 30만톤 내외에 이르고 있으며, 체내 글리코겐 함량이 많고 맛이 좋아지는 11∼3월에 주로 생굴로서 식용하고, 산란 직전인 4∼6월에 수확한 굴은 통조림, IQF 굴(individual quick frozen oyster) 및 건굴 등의 가공원료로 이용하고 있다. 대한민국 공개특허 제0121430호는 수산물 이용한 복합엑스분의 추출방법 및 반응향(reaction flavor)생성 반응을 도입한 풍미증진 방법을 개시하고 있다. 그러나 최근 들어 국내 주요 양식수산물의 하나인 생굴(Oyster)의 전망은 연안 양식장의 오염, Norovirus 검출 및 FDA 권고사항 미이행에 따른 수출 제재 등의 대내외적 요인에 의해 매우 어두운 실정이며, IQF 굴 및 굴 통조림 등 굴 가공품 또한 잉여생산량과 재고량이 더욱 증가할 것으로 예상된다. At present, the annual production of cultured oysters in Korea reaches about 300,000 tons per shell oyster. It is edible as an oyster mainly in November to March when the glycogen content of the body is high and the taste is improved. The harvested oysters are used as raw materials for canning, IQF oysters (individual quick frozen oyster) and gingko. Korean Patent Publication No. 0121430 discloses a method of extracting a compound extract using aquatic products and a method of enhancing a flavor by introducing a reaction flavor generating reaction. However, the outlook for Oyster, one of the major aquaculture products in Korea, is very dark due to internal and external factors such as contamination of coastal farms, Norovirus detection, and export sanctions due to the failure of FDA recommendation. IQF oysters and canned oysters Ore processing and surplus production and inventory are expected to increase further.

본 발명은 상기와 같은 문제점을 포함하여 여러 문제점을 해결하기 위한 것으로서, 년 중 원료로 이용 가능한 IQF(individual quick frozen oyster)굴을 원료로 하여 위생적으로 안전하며, 상온에서 장기간 유통가능 레토르트파우치 굴국(Retort pouched oyster soup)을 제공하는 것을 목적으로 한다. 그러나 이러한 과제는 예시적인 것으로, 이에 의해 본 발명의 범위가 한정되는 것은 아니다.DISCLOSURE Technical Problem The present invention has been made to solve the various problems including the above problems, and it is an object of the present invention to provide a retort pouch refinishing station which is hygienically safe using IQF (individual quick frozen oyster) Retort pouched oyster soup. However, these problems are exemplary and do not limit the scope of the present invention.

본 발명의 일 관점에 따르면, 굴의 열수추출물 및 열수추출잔사를 단백질 가수분해 효소로 처리하여 농축한 잔사효소분해물을 포함하는 굴 복합추출물 2 내지 4 중량부; 굴 15 내지 20 중량부; 마른멸치, 무, 대파, 다시마, 정제염, 정제수 및 굴 자숙액을 혼합 가열하여 제조된 기본육수 60 내지 70 중량부; 및 미역, 무, 찜용 콩나물, 마늘 및 홍고추로 구성되는 군으로부터 선택되는 하나 또는 그 이상의 부재료 10 내지 15 중량부를 레트로트파우치에 충전하고 밀봉한 후, 가열 살균하여 제조되는 레토르트파우치 굴국이 제공된다. According to one aspect of the present invention, there is provided a pharmaceutical composition comprising 2 to 4 parts by weight of an oyster complex extract comprising a hydrolyzed extract of oysters and a residual enzyme hydrolyzate obtained by treating a hydrolysis residue with a protein hydrolyzing enzyme; 15 to 20 parts by weight oyster; 60 to 70 parts by weight of basic broth prepared by mixing hot anchovy, radish, green onion, kelp, refined salt, purified water and oyster juice; And 10 to 15 parts by weight of one or more members selected from the group consisting of seaweed, radish, steamed bean sprouts, garlic and red pepper are filled in a retort pouch, sealed, and then sterilized by heating.

상기 기본육수는 마른멸치 1 내지 2 중량부, 무 3 내지 4 중량부, 대파 0.5 내지 1 중량부, 다시마 0.5 내지 1.5 중량부, 정제염 1 내지 2, 굴 자숙액 5 내지 10 중량 및 잔부의 정제수를 혼합, 가열하여 제조될 수 있다. The basic broth is 1 to 2 parts by weight of dried anchovies, 3 to 4 parts by weight of cornstalks, 0.5 to 1 part by weight of cornstalks, 0.5 to 1.5 parts by weight of sea tangle, 1 to 2 of purified salts, 5 to 10% Mixing, and heating.

본 발명의 또 다른 관점에 따르면, 냉동굴을 반해동 및 3% 식염수로 세척하고 살짝 데치는 굴 준비단계;According to still another aspect of the present invention, there is provided a method for preparing an oyster comprising the steps of: preparing a oyster which is washed with copper and 3% saline,

상기 굴 15 내지 20 중량부에 마른멸치, 무, 대파, 다시마, 정제염, 정제수 및 굴 자숙액을 혼합 가열하여 제조된 기본육수 60 내지 70 중량부, 굴의 열수추출물 및 열수추출잔사를 단백질 가수분해 효소로 처리하여 농축한 잔사효소분해물을 포함하는 굴 복합추출물 2 내지 4 중량부 및 미역, 무, 찜용 콩나물, 마늘 및 홍고추로 구성된는 군으로부터 선택되는 하나 또는 그 이상의 부재료 10 내지 15 중량부를 레토르트파우치에 충전하는 재료 충전단계;60 to 70 parts by weight of basic broth prepared by mixing and heating the dried anchovy, radish, kelp, kelp, purified water, purified water and oyster juice to 15 to 20 parts by weight of the oyster, the hydrolyzed extract of hot water and the hot- 2 to 4 parts by weight of an oyster complex extract containing a residual enzyme hydrolyzate concentrated by treatment with an enzyme and 10 to 15 parts by weight of one or more ingredients selected from the group consisting of seaweed, radish, steamed bean sprouts, garlic and red pepper are added to a retort pouch Filling material charging step;

상기 충전된 레토르트파우치를 밀봉하고 고온 가압살균하는 살균단계; 및A sterilizing step of sealing and filling the filled retort pouch with hot pasteurization; And

살균된 레토르트파우치를 급냉시키는 냉장단계를 포함하는 제1항의 레토르트파우치 굴국의 제조방법이 제공된다. A method of making a retort pouch refractory of claim 1, comprising a refrigeration step of quenching the sterilized retort pouch.

상기 제조방법에 있어서, 상기 충전용기는 레토르트파우치 또는 통조림용 캔일 수 있다.In the above production method, the filling container may be a retort pouch or can for canning.

상기 멸균은 105 내지 121℃에서 30 내지 75분간 가압살균하여 열처리 살균값(Fo value)이 8∼15분을 만족시키며, 살균 목표온도까지의 레토르트 내부온도의 승온 소요시간(Come-up time)은 10분 이내, 바람직하게는 5∼10분 , 살균 후 중심온도 35℃까지의 냉각 소요시간은 20분 이내, 바람직하게는 5∼20분 이내로 실시할 수 있다.The sterilization is performed by pressurizing sterilization at 105 to 121 ° C for 30 to 75 minutes to obtain a heat treatment sterilization value (Fo value) of 8 to 15 minutes, and the temperature of the retort internal temperature to the sterilization target temperature is brought- 10 minutes, preferably 5 to 10 minutes, and the time required for the cooling to 35 [deg.] C after the sterilization is 20 minutes or less, preferably 5 to 20 minutes.

상기 제조방법은 굴의 열수추출물과 열수추출잔사 효소분해물을 혼합하여 복합추출물을 얻는 것으로 종래 열수추출법에 비하여 현저히 수율이 향상되었으며 상기 복합추출물은 굴국의 정미강화용 풍미소재로 손색이 없고 2~4%의 소량 첨가로 굴국 육수의 맛과 강도를 효율적으로 증진시킬 수 있다. 또한 상기 제조방법에 따라 제조된 레토르트파우치 굴국 시작품의 관능적 품질은 시중 굴 음식전문점의 굴국 및 시판 재첩구과 비교하여도 유의차가 없을 정도로 우수한 풍미를 나타내는 것을 특징으로 한다. In the above method, the hydrolyzed extract of oysters and hydrolyzed hydrolyzed hydrolysates were mixed to obtain a combined extract. The yield of the extract was remarkably improved as compared with the conventional hydrothermal extraction method. %, It is possible to efficiently improve the taste and the strength of the oyster soup. In addition, the sensory quality of the retort pouch noodle prepared according to the above-described method is characterized by a superior flavor such that there is no significant difference in comparison with the noodles of the oyster restaurant and the marketplace.

본 발명은 년 중 이용 가능한 IQF 굴을 이용하여 굴국을 제조하고, 굴 복합추출물 첨가와 레토르트파우치 가열살균을 통하여 굴국 육수의 맛과 강도를 효율적으로 증진시키고 상온에서도 장기간 유통, 저장이 가능한 굴국의 제조방법을 구현할 수 있다. 물론 이러한 효과에 의해 본 발명의 범위가 한정되는 것은 아니다.The present invention relates to a method for producing a gruel by using an available IQF oyster and further improving the taste and strength of the gruel by means of addition of oyster complex extract and heat sterilization of a retort pouch, Method can be implemented. Of course, the scope of the present invention is not limited by these effects.

도 1은 본 발명의 재료로 사용한 IQF 굴 사진이다.
도 2는 IQF 굴로부터 추출한 열수추출과 잔사효소분해물을 혼합하여 복합추출물을 제조하는 공정을 나타낸 추출공정도이다.
도 3은 레트르트파우치 굴국 시작품의 제조과정을 나타낸 제조공정도이다.
BRIEF DESCRIPTION OF THE DRAWINGS FIG.
FIG. 2 is an extraction process diagram showing a process for preparing a complex extract by mixing hot water extraction extracted from IQF oysters and residual enzyme hydrolysates.
Fig. 3 is a manufacturing process diagram showing a manufacturing process of a retort pouch frying prototype.

이하, 실시예 및 실험예를 통하여 본 발명을 더 상세히 설명한다. 그러나 본 발명은 이하에서 개시되는 실시예 및 실험예에 한정되는 것이 아니라 서로 다른 다양한 형태로 구현될 수 있는 것으로, 이하의 실시예 및 실험예는 본 발명의 개시가 완전하도록 하며, 통상의 지식을 가진 자에게 발명의 범주를 완전하게 알려주기 위해 제공되는 것이다. Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. It should be understood, however, that the invention is not limited to the disclosed embodiments and examples, but may be embodied in many different forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, It is provided to fully inform the owner of the scope of the invention.

실시예 1: 굴 복합 추출물의 제조 Example 1: Preparation of Oyster Complex Extract

1-1: 추출 단계1-1: Extraction step

본 발명의 일 실시예에 따른 굴 복합 추출물은 IQF 굴에 원료에 물을 가하여 열수추출하고 상기 열수 추출 잔사에 효소를 처리하여 잔사효소분해물을 얻은 후 열수추출물과 잔사효소분해물을 혼합하여 이하 굴국에 이용하였다. The oyster complex extract according to an embodiment of the present invention is prepared by adding water to a raw material of IQF oysters, subjecting it to hot water extraction, treating the hydrothermal extracted residue with an enzyme to obtain a residual enzyme hydrolyzate, mixing the hydrothermal extract and a residual enzyme hydrolyzate, Respectively.

본 발명에서 사용한 IQF 굴(체장 : 7.5~8.5cm, 체중 : 29.5~33.0 g)은 경남 통영시 소재의 대흥물산(주)으로부터 구입하여 -20℃ 동결고에 저장하면서 실험에 사용하였다(도 1). 열수추출잔사 효소분해물의 제조에 사용한 상업효소는 플라보자임(Flavourzyme)으로 노보 Co.(Novonordisk Bioindustrials, Inc., Denmark)에서 구입하여 사용하였고 레토르트파우치 굴국 제조에 사용된 부원료 및 각종 첨가물은 인근 마켓에서 구입하여 사용하였다.The IQF oysters (length: 7.5 to 8.5 cm, body weight: 29.5 to 33.0 g) used in the present invention were purchased from Daeheung Chemical Co., Ltd., Tongyang, Gyeongsangnam-do, . The commercial enzyme used in the preparation of the hydrolyzed residue enzyme hydrolyzate was Flavourzyme purchased from Novoordisk Bioindustrials, Inc., Denmark. The additives and various additives used in the preparation of the retort pouch fermented product were purchased from the nearby market .

열수추출물(hot-water extract)Hot-water extract

IQF 굴에 원료 중량의 약 5배량의 물을 가하고, 90~95℃ 열수 중에서 3시간동안 추출한 후 광목으로 만든 여과포를 이용하여 잔사를 분리하였다. 상기 여과 추출액을 방냉한 후 원심분리(5,000×g)하여 잔사를 제거하고, 고형물의 농도를 Brix 20°로 조정한 것을 열수추출물(hot-water extract)로 하였다.About 5 times as much water as the raw material weight was added to the IQF oysters, and the mixture was extracted in hot water at 90 to 95 ° C for 3 hours. Then, the residue was separated using a filter cloth made of a light wood. The filtrate was allowed to cool, centrifuged (5,000 × g) to remove the residue, and the concentration of the solid was adjusted to 20 ° Brix to obtain a hot-water extract.

잔사효소분해물(scrap enzyme hydrolysate)The scrap enzyme hydrolyzate

잔사효소분해물은 상기 열수추출잔사에 대해 2~3배량의 물을 가한 다음 플라보자임(Novo Nordisk Co., Denmark)을 첨가하여 45℃에서 교반하면서 4시간 동안 효소분해 시킨 후 95℃에서 5분간 열처리하여 플라보자임을 불활성화 시켰다. 이어서 이를 방냉한 뒤 원심분리(5,000×g)한 후 농축하여 고형물의 농도를 Brix 20°로 조정한 추출물을 잔사효소분해물(scrap enzyme hydrolysate)로 하였다.The residual enzyme hydrolyzate was subjected to enzymatic degradation with stirring at 45 ° C for 4 hours after addition of 2 to 3 times the amount of water to the hot-water extract residue, followed by addition of Flavobaim (Novo Nordisk Co., Denmark) Heat treatment was performed to inactivate the flavor. Then, it was allowed to cool, centrifuged (5,000 × g), concentrated, and the concentration of the solid was adjusted to 20 ° Brix to obtain a scrap enzyme hydrolyzate.

복합추출물(complex extract)Complex extract

복합추출물은 상기의 열수추출물과 잔사효소분해물을 혼합한 것을 복합추출물(complex extract)로 하였다. 상기 제조한 열수추출물, 잔사효소분해물 및 복합추출물의 추출공정도를 도 2에 나타냈다.
The complex extract was obtained by mixing the hydrothermal extract and the residual enzyme hydrolyzate. The extraction process of the hot-water extract, the residual enzyme hydrolyzate and the complex extract prepared as described above is shown in Fig.

실시예 2: 레토르트파우치 굴국의 제조 Example 2: Preparation of retort pouch steak

상기 제조된 굴 복합추출물을 이용하여 제조한 레토르트파우치 굴국 시작품 의 제조공정을 도 3에 나타냈다.FIG. 3 shows the production process of the retort pouch starting product prepared using the oyster complex extract prepared above.

먼저, IQF 굴을 반해동시킨 후 3% 식염수로써 점액을 깨끗이 제거한 후, 끓는 물에 담그어 블랜칭(blanching) 처리를 한 다음 102 g 씩 용량 600 mL의 포장용 레토르트파우치에 충전하고, 여기에 굴국용 육수와 각종 부원료와 첨가물을 추가 첨가하여 밀봉하였다. First, the IQF oysters were inoculated, and the mucus was removed with 3% saline solution. The mucus was removed by blanching in boiling water, and then charged into a packing retort pouch having a capacity of 600 mL with a volume of 102 g. Seaweed, various additives and additives were added and sealed.

상기 밀봉한 파우치는 열수순환식 레토르트를 이용하여 Fo값 10분에 도달하도록 121℃에서 살균처리를 행한 후 급냉한 것을 레토르트파우치 굴국(Retort Pouched Oyster Soup)으로 하였고, 본 시작품을 상온에 저장하면서 이화학적 성분조성 및 관능적 품질검사를 실시하였고 가온저장 중 품질안정성에 대하여 분석하였다. The sealed pouch was subjected to sterilization treatment at 121 DEG C so as to reach an Fo value of 10 minutes using a hot water circulating retort, and then quenched to obtain a retort pouched oyster soup. The retort pouch oyster soup was stored at room temperature, The composition and sensory quality test were carried out and quality stability during storage was analyzed.

실시예 2: 굴국용 육수의 제조 Example 2: Preparation of broth for excrement

굴 복합추출물(Brix 10°)의 레토르트파우치 굴국용 육수는 마른멸치 1.6 중량부, 무 3.5 중량부, 대파 0.8 중량부, 다시마 0.9 중량부, 정제염 1.2 중량부, 정제수 84.0 중량부 및 굴 자숙액 8.0 중량부를 혼합하여 제조하였다.
1.6 parts by weight of dried anchovies, 3.5 parts by weight of non-dried anchovies, 0.8 parts by weight of lobster, 0.9 parts by weight of sea tangle, 1.2 parts by weight of purified salt, 84.0 parts by weight of purified water and 8.0 parts by weight of oyster juice 8.0 By weight.

실험예 1: IQF 냉동굴 원료의 일반성분 조성분석Experimental Example 1: Analysis of general composition of IQF frozen oyster raw material

IQF 냉동굴의 일반성분의 조성은 상법(KSFSN, 2000)에 따라, 수분 함량은 상압가열건조법, 조단백질 함량은 semi-micro Kjeldahl법, 조지방 함량은 Soxhlet법 및 회분 함량은 건식회화법으로 측정하였다. 탄수화물 함량은 100에서 수분, 조단백질, 조지방 및 회분의 함량을 뺀 값으로 나타내었다. IQF 냉동굴 원료의 일반성분 조성 분석 결과는 하기 표 1에 나타내었다. IQF 냉동굴 원료의 수분함량은 80.2%, 조단백질 12.4%, 회분 1.0%, 조지방 0.4% 및 탄수화물 함량은 6.0% 이었다. The contents of general components of IQF frozen oysters were measured by the conventional method (KSFSN, 2000), moisture content was measured by atmospheric pressure drying method, crude protein content was measured by semi-micro Kjeldahl method, crude fat content by Soxhlet method and ash content by dry method. The carbohydrate content was expressed as 100 minus the content of moisture, crude protein, crude fat and ash. The general compositional analysis results of the IQF frozen oyster raw materials are shown in Table 1 below. Moisture content of IQF frozen oysters was 80.2%, crude protein 12.4%, ash 1.0%, crude fat 0.4% and carbohydrate content 6.0%.

재료material 함량 (g/100g)Content (g / 100g) 홍합
mussel
수분moisture 조단백질Crude protein 회분Ash 조지방Crude fat 탄수화물carbohydrate
80.2±0.380.2 ± 0.3 12.4±1.112.4 ± 1.1 1.0±0.11.0 ± 0.1 0.4±0.10.4 ± 0.1 6.0±0.36.0 ± 0.3

실험예 2: 굴 복합 추출물(Brix 20°) 분석Experimental Example 2: Analysis of Oyster Complex Extract (Brix 20 °)

2-1: 일반성분 조성, 염도, 아미노질소(NH2-1: General composition, salinity, amino nitrogen (NH 22 -N) 및 휘발성염기질소(VBN) 함량-N) and volatile basic nitrogen (VBN) content

본 발명의 일 실시예에 따른 풍미강화용 굴 복합추출물(Brix 20°)의 일반성분 조성, 염도, 아미노질소(NH2-N) 및 휘발성염기질소(VBN) 함량을 분석하였다.The general composition, salinity, amino nitrogen (NH 2 -N) and volatile basic nitrogen (VBN) content of the oyster complex extract (Brix 20 °) according to one embodiment of the present invention were analyzed.

우선, pH는 시료를 균질화한 다음 pH meter(Accumet Basic, Fisher Sci. Co., USA)로써 측정하였고, 염도는 염도계(460CP, Istek Co., Korea)로써 측정하였다. 휘발성염기질소는 Conway unit를 사용하는 미량확산법(KSFSN, 2000)으로, 아미노질소 함량은 Formol 적정법(Ohara, 1982)으로 측정하였다. First, the pH was measured with a pH meter (Accumet Basic, Fisher Sci. Co., USA) after homogenizing the sample, and the salinity was measured with a salinity meter (460CP, Istek Co., Korea). Volatile Nitrogen was measured by the microdiffusion method using Conway unit (KSFSN, 2000) and amino nitrogen content was measured by Formol titration method (Ohara, 1982).

그 결과, 하기 표 2에 나타난바와 같이, 조단백질6.6%, pH는 6.1, 염도는 2.2%, 아미노질소 및 휘발성 염기질소 함량은 각각 343.0 및 21.0 mg/100 g이었다 As a result, as shown in Table 2, crude protein was 6.6%, pH was 6.1, salinity was 2.2%, amino nitrogen and volatile basic nitrogen contents were 343.0 and 21.0 mg / 100 g, respectively

추출물*
(g/100g)
extract*
(g / 100 g)
조단백질Crude protein pHpH 염도(%)Salinity (%) 아미노질소
(mg/100g)
Amino nitrogen
(mg / 100g)
휘발성 염기질소 (mg/100g)Volatile Nitrogen (mg / 100g)
열수추출물Hot water extract 5.5±0.25.5 ± 0.2 5.8±0.05.8 ± 0.0 2.3±0.02.3 ± 0.0 281.7±0.2281.7 ± 0.2 19.6±0.119.6 ± 0.1 잔사효소분해물Residue enzyme hydrolyzate 8.5±0.18.5 ± 0.1 6.4±0.16.4 ± 0.1 1.9±0.01.9 ± 0.0 366.0±0.3366.0 ± 0.3 22.9±0.222.9 ± 0.2 복합추출물Complex extract 6.6±0.26.6 ± 0.2 6.1±0.06.1 ± 0.0 2.2±0.02.2 ± 0.0 343.0±0.5343.0 ± 0.5 21.0±0.221.0 ± 0.2

2-2: 수율 및 관능검사2-2: Yield and Sensory Evaluation

굴 추출물과 레토르트파우치 굴국 시작품의 수율은 Brix 20°으로 조정한 열수추출물, 잔사효소분해물 및 복합추출물의 양을 측정하여 원료 1 kg에 대한 회수량(mL)으로 나타내었다. 또한, 패류의 관능적 특성에 익숙하도록 훈련된 9인의 패널을 구성하여 굴 추출물과 레토르트파우치 굴국 시작품의 색조, 맛의 특성 및 강도, 냄새, 그리고 종합기호도와 같은 관능적 기준에 대하여 5단계 평점법(5: 아주 좋음, 4: 좋음, 3: 보통, 2: 나쁨, 1: 아주 나쁨)으로 평가하였다. 관능검사의 결과는 SPSS system (Statistical Package, SPSS Inc. USA)을 이용하여 ANOVA test 및 Duncan's multiple range test로 p<0.05 수준에서 시료간의 유의성을 검정하였다(Han, 1999; Kim & Goo, 2001). The yield of the oyster extract and the retort pouch cryoprotectant was determined by measuring the amount of hot-water extract, residual enzyme hydrolyzate and complex extract adjusted to Brix 20 ° and calculating the recovery (mL) with respect to 1 kg of the raw material. In addition, nine panel members trained to familiarize with the sensory characteristics of shellfishes were used to evaluate the sensory criteria such as color, flavor, strength, smell, and overall acceptability of oyster extract and retort pouch noodle prototype : Very good, 4: good, 3: normal, 2: poor, 1: very bad). The results of the sensory test were analyzed by using ANOVA test and Duncan's multiple range test using SPSS system (Statistical Package, SPSS Inc. USA) (p <0.05).

그 결과, 표 3에 나타난 바와 같이, IQF 굴의 열수추출물과 열수추출잔사 효소분해물을 혼합하여 얻은 복합추출물은 열수추출법에 비해 약 40% 이상의 수율을 증가시킬 수 있었으며, 풍미 면에서 열수추출물과 유의차 없이 비슷하였다.  As a result, as shown in Table 3, the combined extract obtained by mixing the hydrothermal extract of IQF oyster with hydrolyzed hydrolyzate resulted in a yield increase of about 40% or more as compared with the hydrothermal extraction method. It was similar without a car.

추출물

extract

수율
(mL/kg)
yield
(mL / kg)
관능검사
Sensory test
색조hue flavor 냄새smell 종합적 기호도Comprehensive likelihood 열수추출물Hot water extract 805805 3.5±0.3a 3.5 ± 0.3 a 4.2±0.2ba 4.2 ± 0.2 ba 4.5±0.2a 4.5 ± 0.2 a 4.3±0.3a 4.3 ± 0.3 a 잔사효소분해물Residue enzyme hydrolyzate 327327 3.8±0.2b 3.8 ± 0.2 b 3.8±0.3b 3.8 ± 0.3 b 3.8±0.3b 3.8 ± 0.3 b 3.8±0.2b 3.8 ± 0.2 b 복합추출물Complex extract 1,1321,132 3.7±0.2b 3.7 ± 0.2 b 4.0±0.3ab 4.0 ± 0.3 ab 4.0±0.3a 4.0 ± 0.3 a 4.0±0.3a 4.0 ± 0.3 a

5단계평점법(n=9, p<0.05), 5: 매우 좋음=굴 전문점 굴국 기준, 4: 좋음, 3: 보통, 2: 나쁨, 1: 매우 나쁨. 5: Very good = Oyster specialty store base, 4: Good, 3: Normal, 2: Poor, 1: Very poor.

실험예 3: 굴 복합추출물(Brix 10°)의 레토르트파우치 참굴국용 육수에 대한 풍미증진 효과Experimental Example 3: Enhancement effect of retort pouch of oyster complex extract (Brix 10 °) on broth

레토르트파우치 참굴국용 육수에 대한 풍미증진 효과는 풍미, 강도 및 맛으로 나누어 5단계 평점법(풍미 ; 5: 매우 좋음, 4: 좋음, 3: 보통, 2: 나쁨, 1: 매우 나쁨. 강도 ; 5: 매우 강함, 4: 강함, 3: 보통, 2: 약함, 1: 매우 약함)으로 평가하였다. The flavor enhancement effect on the retort pouch tuna broth was divided into flavor, strength, and taste and evaluated according to the 5 step rating method (flavor: 5: very good, 4: good, 3: normal, 2: poor, 1: 5: very strong, 4: strong, 3: moderate, 2: weak, 1: very weak).

그 결과, 하기 표 4에 나타난바와 같이, 굴 복합추출물은 굴국의 정미강화용 풍미소재로서 손색이 없었으며, 2 내지 4% 첨가할 경우 레토르트파우치 굴국 육수의 맛과 강도를 효과적으로 증진시킬 수 있었다.As a result, as shown in the following Table 4, the oyster complex extract was not inferior to the flavor enhancing flavor enhancer, and when 2-4% was added, the taste and strength of the retort pouch oyster soup could be effectively enhanced.

관능검사
Sensory test
기본 육수(Base broth)에 대한 첨가량(%)Addition amount (%) to base broth
00 1.01.0 2.02.0 3.03.0 4.04.0 5.05.0 10.010.0 풍 미Wind 2.5a 2.5 a 2.8±0.2a 2.8 ± 0.2 a 3.8±0.3b 3.8 ± 0.3 b 4.3±0.1c 4.3 ± 0.1 c 4.5±0.2c 4.5 ± 0.2 c 4.0±0.2d 4.0 ± 0.2 d 3.7±0.3e 3.7 ± 0.3 e 강 도burglar 2.5a 2.5 a 2.5±0.1a 2.5 ± 0.1 a 3.7±0.2b 3.7 ± 0.2 b 4.0±0.3c 4.0 ± 0.3 c 4.4±0.2d 4.4 ± 0.2 d 4.4±0.2d 4.4 ± 0.2 d 5.0±0.0d 5.0 ± 0.0 d flavor -- 감칠맛
( + )
Richness
(+)
감칠맛
( +++ )
Richness
(+++)
감칠맛
( +++ )
Richness
(+++)
감칠맛
(++++)
Richness
(++++)
감칠맛
(++++)
Richness
(++++)
감칠맛
(++++)
Richness
(++++)

실험예 4: 레토르트파우치 굴국 시작품 분석Experimental Example 4: Retort pouch culturing prototype analysis

4-1: 원료 및 함량4-1: Raw materials and content

본 발명의 일 실시예에 따른 레토르트파우치 굴국 시작품은 IQF 굴 → 해동 및 3% 식염수 수세 → 블랜칭 → 주, 부원료 파우치 충전 및 밀봉 → 레토르트 살균 → 냉각 → 밀봉부 외관검사 공정 순으로 제조하였다. 레토르트파우치 굴국 시작품의 조성은 주원료인 블랜칭한 IQF 굴 17%, 기본 육수 65.0%, 복합추출물(Brix 10°) 2.0%, 미역 3.0%, 무 5.0%, 내열성 찜용 콩나물 5.0%, 마늘 1.0%의 조성비가 가장 적합하였다(표 5 참조).The retort pouch comminution prototype according to an embodiment of the present invention was manufactured in the order of IQF excavation, defrosting, 3% saline water washing, blanching, filling and sealing of the raw material pouch, sterilization of the retort, cooling and sealing inspection. The composition of the retort pouch noodle product was composed of 17% of blanched IQF oysters, 65.0% of basic oysters, 2.0% of combined extract (Brix 10 °), 3.0% of sea mustard, 5.0% of no seaweed, 5.0% of heat resistant soybean sprouts, 1.0% (See Table 5).

원 료Raw material 조성비(%)  Composition ratio (%) IQF 굴IQF oyster 17.017.0 기본육수Basic seaweed 65.065.0 굴 복합추출물 (Brix 10°)Oyster complex extract (Brix 10 °) 2.02.0 미 역Seaweed 3.03.0 radish 5.05.0 찜용 콩나물Bean sprouts 5.05.0 마 늘garlic 1.01.0 홍고추 Red pepper 2.02.0

4-1: 일반성분, pH 및 염도4-1: General composition, pH and salinity

레토르트파우치 굴국 시작품의 일반성분, pH 및 염도를 측정하였다. 그 결과, 하기 표 6에 나타난 바와 같이, 수분함량은 91.0%, 조단백질 2.2% 회분 0.7%, 조지방 0.3%, 탄수화물 5.7%, pH는 6.2 염도는 0.8% 이었다. The general components, pH and salinity of the retort pouch - noodle prototype were measured. As a result, as shown in Table 6, moisture content was 91.0%, crude protein content was 0.7%, crude fat was 0.3%, carbohydrate was 5.7%, and pH was 6.2%.

일반성분(g/100 g)
General composition (g / 100 g)
pH
pH
염도
(%)
Salinity
(%)
수분moisture 조단백질Crude protein 회 분Ash 조지방Crude fat 탄수화물carbohydrate 91.0±0.191.0 ± 0.1 2.3±0.22.3 ± 0.2 0.7±0.10.7 ± 0.1 0.3±0.00.3 ± 0.0 5.7±0.25.7 ± 0.2 6.2±0.16.2 ± 0.1 0.8±0.00.8 ± 0.0

4-2: 총아미노산 및 무기질 조성4-2: Total Amino Acid and Mineral Composition

총아미노산의 조성은 상기 시료에 6.0N HCl 용액을 혼합하고, 히팅 블록(heating block, HF 100, Yamato Co., Japan)으로 24시간 분해시킨 후 감압건조하고, 시트르산 나트륨 완충액(pH 2.20, 0.20 M)로 정용한 후 아미노산 자동분석계(Biochrom 30, Biochrom. LTD, England)를 이용하여 측정하였다.The composition of the total amino acids was obtained by mixing 6.0 N HCl solution with the above sample and digesting with a heating block (HF 100, Yamato Co., Japan) for 24 hours, drying under reduced pressure, sodium citrate buffer (pH 2.20, 0.20 M ), And then measured using an amino acid automatic analyzer (Biochrom 30, Biochrom., LTD, England).

무기질의 조성은 상기시료를 진한 질산용액과 함께 습식회화(Ohara, 1982-b)시킨 후 ashless filter paper로 여과하여 일정량으로 정용한 다음, 유도 결합 플라스마 원자 방출 분광계(Inductively coupled plasma atomic emission spectrometer, ICP, Atomscan 25, TJA, USA)를 이용하여 K, Ca, Mg, Na, Fe, Zn, P, S, Se, Pb 및 Cd의 함량을 분석하였다. The composition of the inorganic material was measured by wet-painting (Ohara, 1982-b) with a concentrated nitric acid solution, filtered with an ashless filter paper, and then quantitated using an inductively coupled plasma atomic emission spectrometer (ICP, Na, Fe, Zn, P, S, Se, Pb, and Cd contents were analyzed by using Atomscan 25, TJA, USA.

그 결과, 하기 표7에 나타난 바와 같이, 총아미노산의 함량은 2,163.8 mg/100 g으로서 글루탐산(Glutamic acid), 아스파르트산(Aspartic acid), 프롤린(Proline), 류신(Leucine), 라이신(Lysine) 및 아르기닌(Arginine)의 주성분이었다. 그 외 다른 필수아미노산들도 고루 함유되어 있어 영양학적으로도 우수하였다. 또한, 무기질의 함량은 나트륨, 칼륨 및 황이 각각 715.5, 70.4 및 70.9 mg/100 g으로 다량 함유되어 있었다. As a result, as shown in the following Table 7, the total amino acid content was 2,163.8 mg / 100 g and the content of glutamic acid, aspartic acid, proline, leucine, lysine, It was the main component of arginine. It also contains other essential amino acids. The contents of minerals were 715.5, 70.4 and 70.9 mg / 100 g, respectively, of sodium, potassium and sulfur, respectively.

아미노산amino acid 함 량 (mg/100 g)Content (mg / 100 g) 무기질Minerals 함 량(mg/100 g)Content (mg / 100 g) AspAsp 200.5200.5 ( 9.3)(9.3) KK 70.4ㅁ1.070.4 ㅁ 1.0 ThrThr 123.8123.8 ( 5.7)(5.7) CaCa 15.2ㅁ0.215.2 ㅁ 0.2 SerSer 117.9 117.9 ( 5.5)(5.5) MgMg 20.8ㅁ0.320.8 ㅁ 0.3 GluGlu 355.1 355.1 ( 16.4)(16.4) NaNa 715.5ㅁ8.3715.5 ㅁ 8.3 ProPro 168.8168.8 ( 7.7)(7.7) FeFe 11.6ㅁ0.111.6 ㅁ 0.1 GlyGly 119.7119.7 ( 5.5)(5.5) ZnZn 2.2ㅁ0.12.2 ㅁ 0.1 AlaAla 135.1135.1 ( 6.2)(6.2) PP 26.9ㅁ0.126.9 ㅁ 0.1 CysCys 16.7 16.7 ( 0.8)(0.8) SS 70.9ㅁ1.770.9 ㅁ 1.7 ValVal 102.0102.0 ( 4.7)(4.7) SeSe trtr MetMet 64.2 64.2 ( 3.0)(3.0) PbPb 0.1ㅁ0.00.1 ㅁ 0.0 IleIle 4.1 4.1 ( 0.2)(0.2) CdCD NDND LeuLeu 171.0171.0 ( 7.9)(7.9) TyrTyr 92.192.1 ( 4.3)(4.3) PhePhe 106.9106.9 ( 5.0)(5.0) HisHis 56.756.7 ( 2.6)(2.6) LysLys 167.2167.2 ( 7.7)(7.7) ArgArg 162.0162.0 ( 7.5)(7.5) 합 계Sum 2,163.82,163.8 (100.0)(100.0)

4-2: 유리아미노산 조성4-2: Free amino acid composition

레토르트파우치 굴국 시작품의 유리 아미노산 조성을 조사하기 위해 시료에 3배량의 70% ethanol 용액을 가하여 균질기(Ultra Turrax T25, IKA, Janke & Kunkel GmbH & Co., Germany)로써 균질화한 후 17,000×g 에서 15분간 원심분리하였다. 이 상층액과 이 조작을 2회 더 반복하여 얻은 상층액을 모아 감압농축한 후 증류수로써 일정량으로 정용하였고, 여기에 제단백을 위해 5'-sulfosalicylic acid를 10% 정도 첨가하여 하룻밤 방치 및 여과한 후 정미성분 분석용 엑스분으로 사용하였다. 유리아미노산 및 관련화합물은 시료 엑스분을 일정량 취해 감압건조한 다음 구연산 리튬 완충액(lithium citrate buffer, pH 2.20, 0.20 M)로서 정용한 후 아미노산 자동분석계(Biochrom 30, Biochrom. LTD, England)로써 분석하였다. In order to investigate the free amino acid composition of the retort pouch cryoprotectant, three times 70% ethanol solution was added to the sample and homogenized with a homogenizer (Ultra Turrax T25, IKA, Janke & Kunkel GmbH & Co., Germany) Minute centrifugation. This supernatant and this operation were repeated two more times. The supernatant was collected and concentrated under reduced pressure. The supernatant was then diluted with distilled water to a certain amount. 5% of 5'-sulfosalicylic acid was added to the supernatant and the mixture was allowed to stand overnight and filtered The extracts were used as extracts for analysis of post-tasting ingredients. The free amino acids and related compounds were analyzed by an amino acid automatic analyzer (Biochrom 30, Biochrom. LTD, England) after a certain amount of sample extract was dried under reduced pressure and then used as lithium citrate buffer (pH 2.20, 0.20 M).

그 결과 하기 표 8에 나타난 바와 같이, 유리아미노산의 총함량은 482.9 mg/100 g으로서 타우린(Taurine), 히드록시프롤린(Hypro), 글루타민산(Glutamic acid), 프롤린(Proline), 류신(Leucine), 히스티딘(Histidine) 및 아르기닌 (Arginine) 등의 함량이 많았다. As a result, as shown in Table 8, the total content of free amino acids was 482.9 mg / 100 g, and the content of taurine, hydroxyprolyne (Hypro), glutamic acid, proline, leucine, Histidine and arginine contents were high.

아미노산amino acid 함 량(mg/100 g)Content (mg / 100 g) 아미노산amino acid 함 량(mg/100 g)Content (mg / 100 g) PhserPhser 1.81.8 ( 0.4)(0.4) CysthCysth 0.30.3 ( 0.1)(0.1) TauTau 41.941.9 ( 8.7)(8.7) IleIle 2.32.3 ( 0.5)(0.5) UreaUrea 15.215.2 ( 3.1)(3.1) LeuLeu 3.23.2 ( 0.7)(0.7) AspAsp 10.910.9 ( 2.3)(2.3) TyrTyr 2.32.3 ( 0.5)(0.5) HyproHypro 280.0280.0 ( 58.0)(58.0) β-alabeta -la 2.72.7 ( 0.5)(0.5) ThrThr 5.45.4 ( 5.4)(5.4) PhePhe 2.22.2 ( 0.4)(0.4) SerSer 4.34.3 ( 4.3)(4.3) GABAGABA 1.41.4 ( 0.3)(0.3) AsnAsn 2.22.2 ( 2.2)(2.2) EthaminEthamin 2.72.7 ( 0.6)(0.6) GluGlu 28.328.3 ( 5.9)(5.9) AmmAmm 4.84.8 ( 1.0)(1.0) AAAAAAAA 1.51.5 ( 0.3)(0.3) HylysHylys 0.30.3 ( 0.1)(0.1) ProPro 21.821.8 ( 4.5)(4.5) OrnOrn 0.90.9 ( 0.2)(0.2) GlyGly 6.86.8 ( 1.4)(1.4) LysLys 6.56.5 ( 1.3)(1.3) AlaAla 10.410.4 ( 2.1)(2.1) HisHis 4.34.3 ( 0.9)(0.9) AABAAABA 0.30.3 ( 0.1)(0.1) ArgArg 12.812.8 ( 2.6)(2.6) ValVal 3.43.4 ( 0.7)(0.7) CysCys 0.40.4 ( 0.1)(0.1) MetMet 1.41.4 ( 0.3)(0.3) 합 계Sum 482.9482.9 (100.0)(100.0)

Phser:phosphoserine, AAAA:α-Amino-adipic acid, AABA:α-Amino-isobutyric acid, Cysth: Cystathionine, GABA:γ-Amino-butyric acid.Phosphor: phosphoserine, AAAA: α-Amino-adipic acid, AABA: α-Amino-isobutyric acid, Cysth: Cystathionine, GABA: γ-Amino-butyric acid.

4-3: 관능검사4-3: Sensory evaluation

레토르트파우치 굴국 시작품의 관능검사를 위하여 레토르트파우치 굴국, 전문점 굴국 및 시판 재첩국을 대상으로 하여 맛, 색조, 냄새 및 종합 기호도를 5단계 평점법(5: 아주 좋음, 4: 좋음, 3: 보통, 2: 나쁨, 1: 아주 나쁨)으로 평가하였다. 관능검사의 결과는 SPSS 시스템(Statistical Package, SPSS Inc. USA)을 이용하여 ANOVA test 및 Duncan's multiple cange test로 p<0.05 수준에서 시료간의 유의성을 검정하였다(Han, H.S. 1999. Statistic Data Analysis. Chungmungak, Seoul; Kim, K.O., Kim, S.S., Sung, R.K. and Lee, Y.C. 1993. Sensory Evaluation Method and Application. Sinkwang Pub. Co., Seoul).Taste, color, smell and general preference were evaluated by 5 step evaluation method (5: very good, 4: good, 3: normal, 2: poor, 1: very bad). The results of sensory evaluation were analyzed by using ANOVA test and Duncan's multiple cange test using SPSS system (Statistical Package, SPSS Inc. USA) (Han, HS 1999. Statistical Data Analysis. Chungmungak, Kim, SS, Sung, RK and Lee, YC 1993. Sensory Evaluation Method and Application, Sinkwang Pub. Co., Seoul).

그 결과, 하기 표 9에 나타난 바와 같이, 본 레토르트파우치 굴국 시작품의 관능적 품질은 시중 굴 음식전문점의 굴국 및 시판 재첩국과 비교하여 유의차가 없을 정도로 풍미 면에서 손색이 없었다.As a result, as shown in Table 9 below, the sensory quality of the retort pouch noodle product did not show any significant difference in flavor as compared with the noodle shop and commercial noodle shop of the oyster food restaurant.


관능검사
Sensory test
flavor 색 조Color tone 냄 새smell 종합기호도Comprehensive preference map 레토르트파우치 굴국Retort pouch refrain 4.4±0.4a 4.4 ± 0.4 a 4.2±0.2a 4.2 ± 0.2 a 4.5±0.3a 4.5 ± 0.3 a 4.5±0.2a 4.5 ± 0.2 a 전문점 굴국A specialty shop 5.05.0 5.05.0 5.05.0 5.05.0 시판 재첩국A commercial copy book 4.0±0.3b 4.0 ± 0.3 b 4.5±0.2a 4.5 ± 0.2 a 4.0±0.1b 4.0 ± 0.1 b 4.2±0.2b 4.2 ± 0.2 b

5단계평점법(n=9, p<0.05), 5: 매우 좋음=굴 전문점 굴국 기준, 4: 좋음, 3: 보통, 2: 나쁨, 1: 매우 나쁨. 5: Very good = Oyster specialty store base, 4: Good, 3: Normal, 2: Poor, 1: Very poor.

4-3: 가온검사4-3: Heating test

가온검사는 식품공전의 레토르트식품 가온보존시험법(식품공전, 2012)에 따라 레토르트파우치 굴국 시작품을 포장 그대로 55±1℃의 incubator(JS-OV-175, Johnsam, Co., Korea)에서 30일간 보존한 후, 상온에서 1일간 추가로 방치하면서 외관 상태를 육안 검사하였다. 정상인 경우 잔존 생균수 측정과 관능검사를 실시하였다. 또한, 생균수는 A.P.H.A.의 표준한천평판배양법(APHA, 1970)에 따라 37±0.5℃에서 24∼48시간 배양하여 나타난 집락수를 계측하였고, 배지는 표준한천평판배지를 사용하였다.The warming test was carried out for 30 days in an incubator (JS-OV-175, Johnsam, Co., Korea) at 55 ± 1 ° C as it was in the package as it was in the retort pouch preservation test method (Food Promotion, 2012) After preservation, it was left standing at room temperature for one day, and the visual appearance was visually inspected. The remaining viable counts and sensory evaluation were normal. The number of viable cells was measured at 37 ± 0.5 ° C for 24 to 48 hours according to standard agar plate culture method (APHA, 1970) of A.P.H.A., and the number of colonies was measured. Standard agar plate medium was used as medium.

그 결과, 하기 표 10에 나타난 바와 같이, 55±1℃ 가온저장 30일 동안 잔존 세균수는 음성, 포장재 팽창 및 내용물 이상 현상 등이 발생하지 않았고, 관능검사 결과 제조 직후와 같은 관능적 품질이 그대로 유지되었는데, 이로 미루어 본 레토르트파우치 굴국은 상온에서 장기간 저장 및 유통이 가능할 것으로 기대되었다.As a result, as shown in the following Table 10, the number of remaining bacteria was not evident during 30 days of storage at 55 ± 1 ° C, no swelling of the packaging material, no abnormal contents, etc., and sensory quality , And it was expected that the retort pouch steak could be stored and distributed for a long time at room temperature.

저장
일수
Save
Days
생균수
(CFU/g)
Viable cell count
(CFU / g)
외관
검사
Exterior
inspection
내용물
검 사
contents
Inspection
관능검사
Sensory test
flavor 냄새smell 종합기호도Comprehensive preference map 00 미검출Not detected 정 상normal 정 상normal 3.8±0.2a 3.8 ± 0.2 a 4.5±0.1a 4.5 ± 0.1 a 4.2±0.2a 4.2 ± 0.2 a 3030 미검출Not detected 정 상normal 정 상normal 3.6±0.3a 3.6 ± 0.3 a 4.5±0.3a 4.5 ± 0.3 a 4.0±0.3a 4.0 ± 0.3 a

본 발명은 도면에 도시된 실시예를 참고로 설명되었으나 이는 예시적인 것에 불과하며, 당해 기술분야에서 통상의 지식을 가진 자라면 이로부터 다양한 변형 및 균등한 다른 실시예가 가능하다는 점을 이해할 것이다. 따라서 본 발명의 진정한 기술적 보호 범위는 첨부된 특허청구범위의 기술적 사상에 의하여 정해져야 할 것이다.
While the present invention has been described with reference to exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the invention. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

Claims (3)

굴의 열수추출물 및 열수추출잔사를 단백질 가수분해 효소로 처리하여 농축한 잔사효소분해물을 포함하는 굴 복합추출물 2 내지 4 중량부; 굴 15 내지 20 중량부; 마른멸치, 무, 대파, 다시마, 정제염, 정제수 및 굴 자숙액을 혼합 가열하여 제조된 기본육수 60 내지 70 중량부; 및 미역, 무, 찜용 콩나물, 마늘 및 홍고추로 구성되는 군으로부터 선택되는 하나 또는 그 이상의 부재료 10 내지 15 중량부를 레트로트파우치에 충전하고 밀봉한 후, 가열 살균하여 제조되는, 레토르트파우치 굴국.2 to 4 parts by weight of an oyster complex extract containing a hydrolyzed extract and a hydrolyzed enzyme residue and concentrated residue enzyme hydrolyzate; 15 to 20 parts by weight oyster; 60 to 70 parts by weight of basic broth prepared by mixing hot anchovy, radish, green onion, kelp, refined salt, purified water and oyster juice; And 10 to 15 parts by weight of one or more materials selected from the group consisting of seaweed, radish, steamed bean sprouts, garlic and red pepper are filled in a retort pouch, sealed and then sterilized by heating. 제1항에 있어서,
상기 기본육수는 마른멸치 1 내지 2 중량부, 무 3 내지 4 중량부, 대파 0.5 내지 1 중량부, 다시마 0.5 내지 1.5 중량부, 정제염 1 내지 2 중량부, 굴 자숙액 5 내지 10 중량 및 잔부의 정제수를 혼합, 가열하여 제조되는, 레토르트파우치 굴국.
The method according to claim 1,
The basic broth is 1 to 2 parts by weight of dried anchovies, 3 to 4 parts by weight of corn steep liquor, 0.5 to 1 part by weight of lobster, 0.5 to 1.5 parts by weight of kelp, 1 to 2 parts by weight of purified salt, 5 to 10 parts by weight of oyster juice, A retort pouch noodle prepared by mixing and heating purified water.
냉동굴을 반해동 및 3% 식염수로 세척하고 살짝 데치는 굴 준비단계;
상기 굴 15 내지 20 중량부에 마른멸치, 무, 대파, 다시마, 정제염, 정제수 및 굴 자숙액을 혼합 가열하여 제조된 기본육수 60 내지 70 중량부, 굴의 열수추출물 및 열수추출잔사를 단백질 가수분해 효소로 처리하여 농축한 잔사효소분해물을 포함하는 굴 복합추출물 2 내지 4 중량부 및 미역, 무, 찜용 콩나물, 마늘 및 홍고추로 구성되는 군으로부터 선택되는 하나 또는 그 이상의 부재료 10 내지 15 중량부를 레토르트파우치에 충전하는 재료 충전단계;
상기 충전된 레토르트파우치를 밀봉하고 Fo값 8분 내지 15분으로 레토르트살균하는 살균단계를 포함하는 제1항의 레토르트파우치 굴국의 제조방법.
The frozen oysters were washed with copper and 3% saline solution, and the oyster preparation step was carried out.
60 to 70 parts by weight of basic broth prepared by mixing and heating the dried anchovy, radish, kelp, kelp, purified water, purified water and oyster juice to 15 to 20 parts by weight of the oyster, the hydrolyzed extract of hot water and the hot- 2 to 4 parts by weight of an oyster complex extract containing a residual enzyme hydrolyzate concentrated by treatment with an enzyme and 10 to 15 parts by weight of one or more ingredients selected from the group consisting of seaweed, radish, steamed bean sprouts, garlic and red pepper are added to a retort pouch A material filling step of filling the material;
The method of manufacturing a retort pouch refractory according to claim 1, comprising the step of sealing the filled retort pouch and sterilizing the retort with an Fo value of 8 to 15 minutes.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210031306A (en) * 2019-09-11 2021-03-19 경상국립대학교산학협력단 Manufacturing Method for Retort Pouch of Marsh Snail and Curled Mallow Soup
KR102620559B1 (en) * 2023-09-20 2024-01-02 이재수 How to make oyster soup and oyster soup manufactured thereby

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210031306A (en) * 2019-09-11 2021-03-19 경상국립대학교산학협력단 Manufacturing Method for Retort Pouch of Marsh Snail and Curled Mallow Soup
KR102620559B1 (en) * 2023-09-20 2024-01-02 이재수 How to make oyster soup and oyster soup manufactured thereby

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