KR20150064580A - Kit for improving skin - Google Patents

Abstract

A skin improvement kit according to the present invention can normalize skin with an increased amount of antimicrobial peptides (AMPs) within skin cells by radiating blue/violet light rays at the wavelength of 400 to 500 nm, More specifically, the skin improvement kit can reduce the expression of antimicrobial peptides and the extent of protein S-nitrosylation by radiating the blue/violet light rays, thereby normalizing skin immunization, keratinization, etc. and ameliorating atopic dermatitis, self immunization, psoriasis, ichthyosis, rosacea, epidermolytic hyperkeratosis, pimples, seborrheic dermatitis, pruritus, etc. In addition, the composition according to the present invention can protect normal skin from blue/violet light rays.

Description

Kit for improving skin}

The present disclosure relates to a composition or kit for protecting and improving skin by regulating the expression of a specific gene in skin cells using sunlight.

Solar light (solar energy) consists of radio wave, microwave, infrared ray, visible ray, ultraviolet ray, X-ray and γ-ray in order from the long side of wavelength of light. And as the negative effects of sunlight on skin are known, many products have been developed to protect the skin from such sunlight. However, most of them have been developed focusing on ultraviolet ray shielding technology. As a result, development of the effect of sunlight on visible light such as visible light has been insufficient.

In addition, existing products are mostly technologies that cut off ultraviolet rays, and techniques for using the sunlight itself to improve the skin condition have not been developed.

Korean Patent Publication No. 10-2010-0099849

One aspect of the present invention is to provide a kit for improving the skin using the function of sunlight, specifically the skin of blue / violetite, or a composition for blocking the skin.

In order to accomplish the above object, an embodiment of the present invention is directed to a method for manufacturing a photovoltaic cell, which comprises a blue / violet light irradiating part having a wavelength of 400 to 500 nm in sunlight and irradiating with antimicrobial peptides (AMPs) Wherein the amount of the antimicrobial peptide in the skin is increased when the amount of the antimicrobial peptide is increased compared to the normal skin.

In addition, one embodiment of the present invention includes an effective ingredient that blocks blue / violet light at a wavelength of 400 to 500 nm in sunlight, and the effective ingredient is antimicrobial peptides (AMPs ) ≪ / RTI > is normal in normal skin.

The kit according to the present invention is characterized in that the blue / violet light having a wavelength of 400 to 500 nm includes an irradiated portion, thereby reducing the amount of antimicrobial peptides (AMPs) in skin where the amount of antimicrobial peptides . Specifically, the blue / violet light is irradiated to reduce the expression of the antimicrobial peptide and the degree of S-nitrosilation of the protein to normalize the skin immunity, keratinization and the like, whereby atopic dermatitis, autoimmune, psoriasis, Skin conditions such as skin, epidermal keratinization, acne, seborrheic dermatitis, and itching can be improved. Furthermore, since the amount of the antimicrobial peptide in the skin cells is increased as compared with the normal skin, the skin elasticity of the skin can be improved to improve the wrinkle formation of the skin of the inflammation or immunological diseases, and the moisturizing and whitening effect can be provided. In addition, in the case of skin inflammation or immune disease patients who are reluctant to apply cosmetics, the skin makeup effect can be maximized by irradiating blue / violet light before makeup.

In addition, one embodiment of the present invention includes an effective ingredient that blocks blue / violet light at a wavelength of 400 to 500 nm in sunlight, and the effective ingredient is antimicrobial peptides (AMPs ) ≪ / RTI > is normal in normal skin.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the relative mRNA levels of the antimicrobial peptides HBD-1, HBD-2, HBD-3, LL-37, Psoriasin, Dermcidin and RNase7, And a graph comparing the light source dictionary (cont.) With the post-irradiation (blue).
2, HBD-1, HBD-2, HBD-3, LL-37, and sera asin when treated with poly I: C and plasgelin to induce infection of viruses and bacteria in normal human keratinocytes , Dicycidine, and RNase7 gene expression in a blue / violet light group dictionary (cont.) And after irradiation (Blue).
FIG. 3 shows the results of treatment of Poly I: C to induce infection of viruses and bacteria in normal horny skin cells, and treatment of Nitric oxide scavenger PTIO and RAC scavenger, NAC (Blue) of the HBV-1, HBD-2, HBD-3, LL-37, thaliana, dicamidine and RNase7 gene .
FIG. 4 shows the results of SDS-PAGE gel (SOD-PAGE) comparing the amount of S-nitrosylated protein (SNO-protiens) produced by blue / violet light irradiation on human normal keratinocytes.
FIG. 5 shows the results of SDS-PAGE gels obtained by comparing changes in the amount of S-nitrocerylated protein (SNO-protiens) produced when ultraviolet light was irradiated to human normal keratinocytes.

The term " sunlight " in this specification is the broadest concept including not only ultraviolet rays but also all wavelengths of sunlight such as radio wave, microwave, infrared ray, visible ray, X ray and? Ray. The term "skin cell" as used herein means a cell of a tissue covering the body surface of an animal, and includes not only a tissue covering the body surface of the face or the body but also a cell of the hair .

Hereinafter, the present invention will be described in detail.

One embodiment of the present invention includes a blue / violet light irradiating part having a wavelength of 400 to 500 nm in sunlight, wherein the amount of antimicrobial peptides (AMPs) Which is used for reducing the amount of the antimicrobial peptide of the present invention. At this time, the skin that is increased in the amount of the antimicrobial peptide in the skin cell than the normal skin contains, for example, a skin containing 1.2 times or more the amount of the antimicrobial peptide than the normal skin, or a relatively increased amount of the antimicrobial peptide But is not limited to the amount of the increased amount of the skin.

According to one embodiment, the blue / violet light of the wavelength of 400-500 nm reduces the expression amount of the antimicrobial peptides (AMPs). In addition, the blue / violetite can be used for various cytokines and related genes and proteins such as RANTES (Human CCL5), CCL2 (Chemokine ligand 2), IL-6 (Interleukin-6), IL- 8), TNF-α (Tumor necrosis factor-α), and COX-2 (Cyclooxygenase-2).

Specifically, the antimicrobial peptide is a protein having a small molecular weight and has antibacterial activity to prevent infection by pathogenic bacteria such as bacteria, viruses and fungi. The antimicrobial peptides have been reported to induce angiogenesis, wound healing, and chemotaxis, and have structural features that have both positive and hydrophilic sites while being positively charged. (Antibacterial peptides (AMPs)) include HBD-1 (Human beta-defensin-1, ACCESSION NP_005209, VERSION NP_005029.1 GI: 4885181), Human beta-defensin-2, ACCESSION NP_004933, VERSION NP_004933.1 GI: (Cathelicidin-37, ACCESSION NP_004336, VERSION NP_004336.3 GI: 348041314), HIV-3 (Human beta-defensin-3, ACCESSION AAG02237, VERSION AAG02237.1 GI: 49931627) Accession AAA60210, VERSION AAA60210.1 GI: 190668), dermycidin (ACCESSION NP_444513, VERSION NP_444513.1 GI: 16751921) and ribonucleic acid hydrolase 7 (RNase 7, ACCESSION CAC84458, VERSION CAC84458.1 GI: 40643235 ), And the like.

According to one embodiment, the above-mentioned HBD (human beta-defensin) -1, HBD-2, HBD-3, LH (Cathelicidin) -37, Psoriasin, Dermcidin, ribonucleic acid hydrolase (RNase) 7 is a congenital immune factor present in the conjunctiva of the respiratory tract of the digestive tract, killing Gram positive / negative bacteria and fungal viruses or inhibiting growth. In addition to keratinocytes, they are present in the secretions of the skin's sebaceous glands and sebaceous glands, and are secreted and deposited on the skin surface through sweat and sebum, contributing to the formation of antibacterial barriers. And they show increased levels in patients with atopic dermatitis, psoriasis, autoimmune disease, etc. than normal people. In addition, the antimicrobial peptides exhibit an increase in the expression level when irradiated with ultraviolet light, as opposed to those irradiated with blue / violet. The expression characteristics of the antimicrobial peptide against ultraviolet light are described in Glaser R. et al. UV-B radiation induces expression of antimicrobial peptides in human keratinocytes in vitro and in vivo, J Allergy Clin Immunol. 2009 May; 123 (5): 1117-23. , Which is incorporated herein by reference in its entirety.

S-Nitrosylated proteins increase the expression level upon irradiation with ultraviolet rays. However, according to another embodiment of the present invention, the blue / violetite light having a wavelength of 400 to 500 nm is converted into S- (S-Nitrosylated) protein. Specifically, nitric oxide associated with many functions in vivo can react with active cysteine residues to generate protein S-nitrocellulose, and protein S-nitrosylation, like protein phosphorylation, Cell signaling can be regulated. In addition, the blue / violetite can be obtained by a conventional method such as nitrosylation, that is, de-nitrosylation which removes nitric oxide from a S-nitroxylated protein as described above . And, if the amount of the S-nitrosylated protein produced by Blue / Violet Light is decreased, antimicrobial proteins (AMPs) are also reduced.

The expression level of the antimicrobial peptide gene or the S-nitrosylated protein can be measured by, for example, reverse transcription polymerase chain reaction (RT-PCR) and northern blot analysis, enzyme-linked immunosorbent assay assay, ELISA, radioimmunoassay (RIA), sandwich assay, western blot, immunoblot, immunohistochemical staining, biotin-switch assay, ≪ / RTI > and combinations thereof.

Accordingly, according to one embodiment of the present invention, the skin having the above-mentioned amount of the antimicrobial peptide in the skin cell is increased compared to normal skin, includes inflammation or immunological disease skin. Specifically, skin inflammation or immunological diseases such as atopic dermatitis and psoriasis are caused by an abnormality of the immune system, and exhibit an increased level of expression of antimicrobial peptides (AMPs). In the case of atopic dermatitis, for example, IL-4 (interleukin-4), IL-5 and IL-10 are secreted, and IL-4 has been reported to play an important role in the increase of IgE (Immunoglobulin E) . In addition, the inflammation of the skin as described above inhibits the elasticity of the epidermis, which is a main cause of wrinkles of the skin, and the deposition of melanin pigment due to skin inflammation is increased, and skin aging can be promoted.

Therefore, by irradiating the blue / violet light, the amount of the antimicrobial peptide in the skin is increased as compared with the normal skin, and the degree of S-nitrosilation of the protein and the degree of S- Immunity and keratinization can be improved to improve skin conditions such as atopic dermatitis, autoimmune disease, psoriasis, lice, rosacea, epidermal keratinization, acne, seborrheic dermatitis and itching. Furthermore, it is possible to improve the wrinkle formation of the skin and provide a moisturizing and whitening effect.

On the other hand, skin having a normal amount of antimicrobial peptides in skin cells should prevent the decrease in the amount of antimicrobial peptides due to blue / violetite as described above. Therefore, in another embodiment of the present invention, Of blue / violet light, wherein said active ingredient is a skin protection application for normal skin in which the amount of antimicrobial peptides in the skin cells is normal. In one embodiment, the active ingredient blocking the blue / violetite may be used without limitation as long as it is a component blocking sunlight contained in the sunscreen product. Examples of the active ingredient include titanium oxide (TiO ₂ ), zinc oxide (ZnO) And tinasolve may be used.

In an embodiment of the skin improvement kit of the present invention, the intensity of the blue / violet light is 0.1 mW / cm ² to 10 W / cm ² , more specifically 1 mW / cm ² to 100 mW / cm ² . If the amount is less than the above range, the efficacy is insignificant or not. If the above range is exceeded, pigment deposition, DNA damage and the like may be induced.

In an embodiment of the skin improvement kit of the present invention, the irradiation time of the blue / violet light may be 0.5 to 120 minutes, more specifically 1 to 60 minutes. If the amount is less than the above range, the efficacy is insignificant or not. If the above range is exceeded, pigment deposition, DNA damage and the like may be induced.

In one embodiment, the blue / violet light irradiating portion may comprise a solar light transmitting material. The photovoltaic material may be a quartz plate or a bandpass filter that transmits only a target light beam region. The bandpass filter may be a quartz plate, But the present invention is not limited thereto.

In addition, in one embodiment, the skin-improving kit may include at least one of information for adjusting the intensity of the blue / violet light and information for adjusting the irradiation time of the blue / violet light according to the skin condition of the user As shown in FIG.

For example, since the skin may be more sensitive or weaker than an adult in the case of a younger person (younger than 20 years), it is possible to use the same amount of adult use but more suitably in a light amount of 1/2 to 9/10 The amount of light can be adjusted from 1 J / cm ² to 100 J / cm ² depending on the size, severity and severity of the lesion.

The instructions may further include information on when the blue / violet light is irradiated. The period of use can be used at any time of the day, and it is possible to perform multiple exposures, for example, up to 30 minutes before going out or repeatedly irradiating less than 3 times a day.

Meanwhile, another embodiment of the present invention as described above includes an active ingredient blocking blue / violet light having a wavelength of 400 to 500 nm in sunlight, and the active ingredient may be an antimicrobial peptide (for example, antimicrobial peptides, AMPs) is normal.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to Test Examples, Examples and Comparative Examples. However, these test examples, examples and comparative examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited by the following test examples, examples and comparative examples.

[Test Example 1]

As an embodiment of the present invention, an experiment for measuring changes in the expression level of antimicrobial peptides when irradiating skin cells with blue / violet light having a wavelength of 400 to 500 nm was carried out as follows.

First, the human epidermal neonatal keratinocyte cells used in the present invention are commercially available and are preferably purchased from Lonza (Inc., Walkersville, Maryland, USA) The cells were cultured in a CO ₂ incubator (CO ₂ incubator) at 37 ° C and 5% CO ₂ . The cell culture was cultured according to the Lonza's guidelines. To the 500 ml of KBM-2 (Clonetics CC-3103) medium, 2 ml of a KGM-2 bullet kit (bovine pituitary extract), 0.5 ml of human epidermal growth factor (hEGF), 0.5 ml of insulin, 0.5 ml of Hydrocortisone, 0.5 ml of Transferrin, 0.5 ml of Epinephrine and 0.5 ml of Gentamycin Suflate + Amphofericin-B (GA-1000) were used .

Then, the cultured human normal horny skin cells were irradiated with blue / violetite (10 J / cm ² ) at a wavelength of 400 to 500 nm, the culture medium of human normal horny skin cells was then removed, and 2 ml of phosphate buffer Cells were washed with phosphate buffered saline (PBS) and then RNA was isolated from the cells using a trizol reagent (Invitrogen, Carlsbad, Calif., USA).

(Agilent 2100 BioAnalyzer, Agilent Technologies, Santa Clara, Calif., USA) after purification of the separated RNA with an RNA kit (QIAGEN RNeasykt, QIAGEN, Valencia, CA) Were used to confirm the quality of the RNA. CDNA was synthesized from the RNA using a reverse transcription kit (Superscript Reverse Transcriptase kit, Invitrogen, Carlsbad, CA) of Invitrogen. Real-time reverse PCR transcription polymerase chain reaction).

The expression patterns of HBD-1, HBD-2, HBD-3, LL-37, threonine, dicamidine and RNase7 were measured by TaqMan gene expression assay kit (Applied Biosystems, Foster City, CA) was used to evaluate the genetic changes of skin induced by blue / violet light by real-time PCR. The results are shown in FIG. The primers used for the amplification of each gene are shown in Table 1 below.

Gene name primer HBD-1 Hs00608345_m1 HBD-2 Hs00175474_m1 HBD-3 Hs00218678_m1 LL-37 Hs00189038_m1 Psoriasin Hs00161488_m1 Dermcidin Hs00364976_m1 RNase7 Hs00922963_s1

As a result, as shown in FIG. 1, the congenital immune genes, namely the antimicrobial peptides HBD-1, HBD-2, HBD-3, LL-37, Psoriasin, Dermcidin and RNase 7 And the amount of each gene expression (Relative mRNA Level) decreased after irradiation (Blue). This means that irradiation of blue / violet light can control the amount of antimicrobial peptide expression in skin cells.

[Test Example 2]

As an embodiment of the present invention, an experiment for measuring changes in the expression level of antimicrobial peptides when irradiating skin cells with blue / violet light having a wavelength of 400 to 500 nm was carried out as follows.

First, the human epidermal neonatal keratinocyte cells used in the present invention are commercially available and are preferably purchased from Lonza (Inc., Walkersville, Maryland, USA) The cells were cultured in a CO ₂ incubator (CO ₂ incubator) at 37 ° C and 5% CO ₂ . The cell culture was cultured according to the Lonza's guidelines. To the 500 ml of KBM-2 (Clonetics CC-3103) medium, 2 ml of a KGM-2 bullet kit (bovine pituitary extract), 0.5 ml of human epidermal growth factor (hEGF), 0.5 ml of insulin, 0.5 ml of Hydrocortisone, 0.5 ml of Transferrin, 0.5 ml of Epinephrine and 0.5 ml of Gentamycin Suflate + Amphofericin-B (GA-1000) were used .

Then, the cultured human normal horny skin cells were irradiated with blue / violetite (10 J / cm ² ) at a wavelength of 400 to 500 nm, the culture medium of human normal horny skin cells was then removed, and 2 ml of phosphate buffer Cells were washed with phosphate buffered saline (PBS) and then RNA was isolated from the cells using a trizol reagent (Invitrogen, Carlsbad, Calif., USA).

Poly I: C and plasgelin (Flagellin, Invivogen, San Diego, California, USA) were treated before and after the blue / violetite irradiation (10 J / cm ² ) to induce virus and bacterial infection.

Then, the separated RNA was further purified with an RNA kit (QIAGEN RNeasykt, QIAGEN, Valencia, Calif.) Of Agagen, Agilent 2100 BioAnalyzer, Agilent Technologies, Santa Clara, Calif. , USA) was used to confirm the quality of RNA. CDNA was synthesized from the RNA using a reverse transcription kit (Superscript Reverse Transcriptase kit, Invitrogen, Carlsbad, CA) of Invitrogen. Real-time reverse PCR transcription polymerase chain reaction).

The expression patterns of HBD-1, HBD-2, HBD-3, LL-37, threonine, dicamidine and RNase7 were measured by TaqMan gene expression assay kit (Applied Biosystems, Foster City, CA) was used to evaluate the genetic changes of skin induced by blue / violet light by real-time PCR, and the results are shown in FIG. The primers used for amplification of each gene are shown in Table 1 above.

2, HBD-1, HBD-2, HBD-3, LL-37, and sera asin when treated with poly I: C and plasgelin to induce infection of viruses and bacteria in normal human keratinocytes , Dicamidine, and RNase7 gene expression in a blue / violet light group (cont.) And after irradiation (Blue). As shown in FIG. 2, when the antimicrobial peptides HBD-1, HBD-2, HBD-3, LL-37, threia, dicalin and RNase7 were increased by Poly I: It can be confirmed that the expression amount of each of the genes is reduced by irradiation of violet light. These changes were statistically significant, and the statistics were verified by a two-sided Student t test. Significant differences were noted when the p-value was less than 0.05. This means that irradiation of blue / violet light can control the amount of antimicrobial peptide expression in skin cells.

[Test Example 3]

As an embodiment of the present invention, an experiment for measuring changes in the expression level of antimicrobial peptides when irradiating skin cells with blue / violet light having a wavelength of 400 to 500 nm was carried out as follows.

First, the human epidermal neonatal keratinocyte cells used in the present invention are commercially available and are preferably purchased from Lonza (Inc., Walkersville, Maryland, USA) The cells were cultured in a CO ₂ incubator (CO ₂ incubator) at 37 ° C and 5% CO ₂ . The cell culture was cultured according to the Lonza's guidelines. To the 500 ml of KBM-2 (Clonetics CC-3103) medium, 2 ml of a KGM-2 bullet kit (bovine pituitary extract), 0.5 ml of human epidermal growth factor (hEGF), 0.5 ml of insulin, 0.5 ml of Hydrocortisone, 0.5 ml of Transferrin, 0.5 ml of Epinephrine and 0.5 ml of Gentamycin Suflate + Amphofericin-B (GA-1000) were used .

Then, the cultured human normal horny skin cells were irradiated with blue / violetite (10 J / cm ² ) at a wavelength of 400 to 500 nm, the culture medium of human normal horny skin cells was then removed, and 2 ml of phosphate buffer Cells were washed with phosphate buffered saline (PBS) and then RNA was isolated from the cells using a trizol reagent (Invitrogen, Carlsbad, Calif., USA).

At this time, Poly I: C was treated to induce infection of viruses and bacteria before and after the blue / violetite (10 J / cm ² ) irradiation. In addition, Nitric oxide scavenger PTIO (2- (4-Carboxyphenyl) -4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt) and active oxygen species scavenger (N-acetyl-L-cysteine) (Sigma, St. Louis, MO, USA).

Then, the separated RNA was further purified with an RNA kit (QIAGEN RNeasykt, QIAGEN, Valencia, Calif.) Of Agagen, Agilent 2100 BioAnalyzer, Agilent Technologies, Santa Clara, Calif. , USA) was used to confirm the quality of RNA. CDNA was synthesized from the RNA using a reverse transcription kit (Superscript Reverse Transcriptase kit, Invitrogen, Carlsbad, CA) of Invitrogen. Real-time reverse PCR transcription polymerase chain reaction).

The expression patterns of HBD-1, HBD-2, HBD-3, LL-37, threonine, dicamidine and RNase7 were measured by TaqMan gene expression assay kit (Applied Biosystems, Foster City, CA) was used to evaluate the genetic changes of skin induced by blue / violet light. The results were shown in FIG. The primers used for amplification of each gene are shown in Table 1 above.

As shown in FIG. 3, when the amount of expression of HBD-1, HBD-2, HBD-3, LL-37, thylazine, dummycin and RNase7 was increased by Poly I: C, In the group treated with PTIO, which is a nitric scavenger of the two scavengers, which was reduced by irradiation, the expression level of antimicrobial peptides (AMPs) did not decrease in blue / violet light irradiation. This means that blue / violetite is involved in the production of S-nitrocellulose and in the regulation of the expression of antimicrobial peptides (AMPs).

[Test Example 4]

As an embodiment of the present invention, an experiment for measuring the amount of S-nitrosylated protein produced when irradiating blue / violet light having a wavelength of 400 to 500 nm onto skin cells Was carried out as follows.

First, the human epidermal neonatal keratinocyte cells used in the present invention are commercially available and are preferably purchased from Lonza (Inc., Walkersville, Maryland, USA) The cells were cultured in a CO ₂ incubator (CO ₂ incubator) at 37 ° C and 5% CO ₂ . The cell culture was cultured according to the Lonza's guidelines. To the 500 ml of KBM-2 (Clonetics CC-3103) medium, 2 ml of a KGM-2 bullet kit (bovine pituitary extract), 0.5 ml of human epidermal growth factor (hEGF), 0.5 ml of insulin, 0.5 ml of Hydrocortisone, 0.5 ml of Transferrin, 0.5 ml of Epinephrine and 0.5 ml of Gentamycin Suflate + Amphofericin-B (GA-1000) were used .

The cultured cells were irradiated with 400 to 500 nm of blue / violet light at 10, 30, and 60 J / cm ² , respectively. Then, the culture medium of human normal horny skin cells was removed, and the cells were washed with 2 ml of phosphate buffered saline (PBS) , Followed by a biotin-switch assay for detection of protein S-nitrosylation. Specifically, the washed cells were dissolved in a HENTS solution (HEPES, Triton X100, SDS, protease inhibitor, EDTA) for 10 minutes to take only the supernatant. After quantification of protein, HEN (HEPES, protease inhibitor, EDTA) solution was used to adjust to 600ug / 200ul. MMTS (Sigma) was added to 10 mM to prevent the reaction of S-nonylated cystein, followed by reaction at 50 ° C for 15 minutes. After protein precipitation with acetone, the cells were dissolved in 50 mM ascorbic acid (HEPES, SDS, protease inhibitor, EDTA) containing 1 mM HPDP-biotin (Pierce) and reacted at 37 ° C for 1 hour to obtain S- The biotin was bound to the cysteine. After protein precipitation with acetone, the resultant was dissolved in HENS solution, 5% of the input for calibration was removed, 20 μl each of avidin-agarose bead (Pierce) was added thereto, and the mixture was reacted at 4 ° C. for 12 hours . Wash the beads 5 times with neutralization solution (HEPES, EDTA, NaCl, Triton X 100), add sample buffer, boil and perform SDS-PAGE with input. After that, the protein that was S-nitrosylated through silver staining was identified by SDS-PAGE gel, which is shown in FIG.

As a result, in comparison with the control (cont) in which blue / violet light was not irradiated as shown in FIG. 4, SN-proteins (SN) in vivo increased with increasing blue / violet light irradiation intensity , Respectively. This experiment was conducted under the same conditions and method as above except that ultraviolet rays (20 mJ) were irradiated instead of blue / violet light. As a result, in vivo S-nitrosilated proteins (SNO-proteins) (See FIG. 5). This means that even the same sunlight acts differently on the skin depending on its wavelength, and blue / violet light can reduce the amount of S-nitroxylated protein, unlike ultraviolet light.

Claims (13)

A blue / violet light irradiating part having a wavelength of 400 to 500 nm in the sunlight,
A kit for skin improvement, wherein the amount of antimicrobial peptides (AMPs) in skin cells is used to reduce the amount of the antimicrobial peptide in the skin, which is increased over normal skin. 2. The kit for improving skin according to claim 1, wherein the kit comprises an amount of the antimicrobial peptide in the skin cell of at least 1.2 times the normal skin. The kit according to claim 1, wherein the skin having an increased amount of the antimicrobial peptide in the skin cell than normal skin is an inflammatory or immunological disease skin. The skin improvement kit according to claim 1, wherein the blue / violetite reduces the expression amount of antimicrobial peptides (AMPs) in skin cells. The skin-improving kit according to claim 1, wherein the blue / violetite reduces the amount of S-nitrosylated protein in the skin cells. The skin improvement kit according to claim 1, wherein the intensity of the blue / violet light is 0.1 mW / cm ² to 10 W / cm ² . The skin improvement kit according to claim 1, wherein the irradiation time of the blue / violet light is 0.5 to 120 minutes. The skin improvement kit according to claim 1, wherein the blue / violet light irradiation part comprises a sunlight-permeable substance. The skin improvement kit according to claim 8, wherein the photovoltaic material is a quartz plate or a band pass filter. The skin care kit according to claim 1,
Further comprising a manual that includes at least one of information for adjusting the intensity of the blue / violet light and information for adjusting the irradiation time of the blue / violet light according to the skin condition of the user. Which contains an effective component that blocks blue / violet light at a wavelength of 400 to 500 nm in sunlight,
Wherein the active ingredient is a skin protection application for normal skin in which the amount of antimicrobial peptides (AMPs) in skin cells is normal. 12. The composition of claim 11, wherein the blue / violetite reduces the expression level of antimicrobial peptides (AMPs) in the skin cells. 12. The composition of claim 11, wherein the blue / violetite reduces the amount of S-nitrosylated protein produced in the skin cells.

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