KR20150062577A - A method of extracting natural ingredients of cucumber and cosmetic composition comprising the extracted ingredients - Google Patents

Abstract

The present invention relates to a cosmetic composition containing main ingredients of cucumber and, more specifically to a cosmetic composition containing undestroyed main ingredients of cucumber by controlling the pH of an extraction solution, the content of sodium chlorides, and the content of an organic solvent.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition containing a cucumber extract and a cosmetic composition containing the extract,

The present invention relates to a cosmetic composition containing a major component of cucumber, and more particularly to a cosmetic composition containing a major component of cucumber itself in a non-destructive manner by controlling pH, sodium chloride and organic solvent content of the extract solution .

Cucumbers have been cultivated since the Unified Silla period, and have been used for the treatment of diarrhea and diarrhea in one room. In the Joseon Dynasty, the juice from the cucumbers was used for the facial care, and now the cucumber is applied to the beauty law in various ways in the private sector. For example, cosmetic lotion and oily pack using cucumber are known to have excellent skin convergence and soothing effect. Causes of cucumber's efficacy in skin sedation were ascorbic acid (vitamin C), caffeic acid, alpha-hydroxy acid, cucurbitacin D, 23,24- Dihydrocucurbitacin D, silica, potassium, magnesium, and the like. However, these components may cause destruction during the storage period of the cucumber. Therefore, when the stored cucumber is used or when cutting or dipping in order to use the cucumber, ascorbinase, polyphenoloxidase, Since the breakdown is accelerated by the action of peroxidase, the use of the main components of the cucumber is limited.

Korean Patent Publication No. 2009-0117199 (published on November 12, 2009)

The present inventors have found that by extracting the main components of cucumber by controlling the principle used to enhance the storage stability of Kimchi, specifically the pH of the extract solution, the sodium chloride and the organic solvent content, Can inhibit the activity of the nutrient destroying enzyme of the cucumber itself and prevent the main component of the cucumber from being destroyed, thus completing the present invention.

In order to achieve the above object, the present invention provides a cosmetic composition in which the pH of the extract solution, sodium chloride, and organic solvent content are controlled so that the main component of the cucumber itself is not destroyed.

The present invention relates to a cosmetic composition containing a major component of cucumber. By controlling the pH, the sodium chloride and the organic solvent content of the extract solution, the activity of ascorbinase, polyphenol oxidase and peroxidase is inhibited, Main ingredients can be kept without destroying.

FIG. 1 is a graph showing changes in content of ascorbic acid according to storage period in Example 7 and Comparative Example. FIG.
FIG. 2 is a graph showing changes in content of caffeic acid according to storage period in Example 7 and Comparative Example. FIG.
FIG. 3 is a graph showing changes in the content of cucurbitacin D in storage period according to Example 7 and Comparative Example. FIG.
4 is a graph showing changes in the content of 23,24-dihydrocucurbitacin D in storage period according to Example 7 and Comparative Example.

The present invention relates to a cosmetic composition containing a main component of cucumber, and relates to a cosmetic composition containing main components of the cucumber itself in a non-destructive manner by controlling the pH of the extract solution, the sodium chloride and the content of the organic solvent.

Hereinafter, the present invention will be described more specifically.

The cucumber of the present invention is a perennial plant of the dicotyledonous plant-digesting group and is a plant belonging to the genus Corynebacterium, which contains ascorbic acid (vitamin C), caffeic acid, cucurbitacin D and 23,24-dihydrocucurbitacin D It contains as a main ingredient. The present invention is to disrupt cucumber tissues by controlling the pH, sodium chloride, and organic solvent content of the extract solution, while simultaneously extracting the main components.

The extraction solution used in the present invention contains vinegar, an organic solvent, and sodium chloride. The vinegar may be any of conventional vinegar such as synthetic vinegar, fermented vinegar, fermented vinegar, etc. However, it is more preferable to use vinegar (hereinafter referred to as " The mixture of 93.6% by weight of water, 5.0% by weight of butylene glycol, 0.8% by weight of phenoxyethanol, 0.5% by weight of flower extract of Lonicera japonica (Honeysuckle), ethylcellyl glycerin And 0.1% by weight of Ethylexylglycerin can be used.

The pH of the extraction solution is 3 or more and less than 7, preferably 5. If the pH of the extraction solution is less than 3, there is a limitation in using the cosmetic composition, and if the pH is 7 or more, the enzyme activity may be inhibited.

The sodium chloride used in the present invention may be contained in an amount of 0.5 to 10% by weight, preferably 0.5 to 1.5% by weight based on the total weight of the extraction solution. If the sodium chloride is contained in an amount of less than 0.5 wt%, the effect of the component is insufficient, and if it is contained in an amount of more than 10 wt%, stability of the formulation may become unstable.

The organic solvent used in the present invention is usually ethanol which is usable in cosmetics, but methanol and butanol are not usable. The organic solvent may be contained in an amount of 0 to 30% by weight, preferably 0 to 20% by weight based on the total weight of the extraction solution. This is because if the organic solvent is contained in an amount exceeding 30% by weight, the formulation stability becomes unstable.

The main component of the cucumber of the present invention is obtained by washing the cucumber, cutting it into a certain size, and then adding it to the extract solution having a pH of 3 or more to less than 7 to disrupt the cucumber. Specifically, it is preferable to add 70 g of the extraction solution to 30 g of cucumber, and the extraction solution is preferably composed of 84.3% by weight of vinegar, 14.3% by weight of ethanol and 1.4% by weight of sodium chloride based on the total weight of the extract solution Do.

The composition of the present invention may be formulated as an external preparation for skin, in particular as a cosmetic composition, and may be formulated containing a cosmetically or dermatologically acceptable medium or base. In addition, the composition of the present invention may be provided in any form suitable for topical application, for example, as a solution, an emulsion obtained by dispersing an oil phase in water phase, an emulsion obtained by dispersing water phase in water phase, a suspension, a solid, a gel, Pastes, foams, or aerosol compositions. Compositions of such formulations may be prepared according to conventional methods in the art.

In addition, the composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, to the extent that the main effect is not impaired. The composition according to the present invention may further comprise a humectant, an emollient, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic or inorganic pigment, a perfume, a cold agent or a limiting agent. The compounding amount of the above components can be easily selected by a person skilled in the art within the range not impairing the object and effect of the present invention, and the amount thereof may be from 0.01 to 5% by weight, specifically from 0.01 to 3% by weight based on the total weight of the composition .

In addition, the composition of the present invention can be formulated as a pharmaceutical composition. When the composition according to the present invention is applied to medicines, it may be formulated into oral, parenteral or parenteral dosage forms in solid, semi-solid or liquid form by adding an inorganic or organic carrier compatible with the active ingredient used in the present invention , The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, and the like.

Examples of the formulations for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups and pellets. In addition, the formulations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. The composition according to the present invention can be easily formulated into an active ingredient by carrying out the composition according to a conventional method. In this case, a surfactant, excipient, coloring agent, spice, preservative, stabilizer, buffer, suspending agent, have.

The dosage of the active ingredient of the pharmaceutical composition of the present invention will vary depending on the age, sex, body weight, pathological condition and severity of the subject to be treated, route of administration, or judgment of the prescriber. Determination of the appropriate dose based on these factors is well within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / / Day, but is not limited thereto.

The present invention will be described in more detail with reference to the following examples and test examples. However, the following examples and test examples are provided to aid understanding of the present invention, and the scope of the present invention is not limited in any way by these examples and test examples.

[Test Example 1] Extraction yield of main components of cucumber

The main components of the cucumber were extracted as Comparative Examples and Examples in Table 1 below, and the extraction yields are shown in Table 2 below. The cucumber was washed at room temperature (15 to 25 ° C), cut into a predetermined size, and then mixed with 70 g of the extract solution to 30 g of cucumber using a natural material mixing type cosmetic container to extract main components of the cucumber. , An organic solvent, and sodium chloride, and ethanol as an organic solvent. The ginseng vinegar comprises 93.6% by weight of water, 5.0% by weight of butylene glycol, 0.8% by weight of phenoxyethanol, 0.5% by weight of Lonicera japonica (Honeysuckle flower extract) , And 0.1% by weight of Ethyllexylglycerin. The extraction yield was determined by centrifuging the extract solution in a high-speed centrifuge (10,000 rpm) for 10 minutes and injecting the supernatant into high performance liquid chromatography (HPLC) The pH of the extract solution, sodium chloride and ethanol were kept unchanged in the aqueous solution state, and the extraction yield in the comparative example was 100 (unit: wt%).

The pH of the extraction solution Sodium chloride (%) ethanol(%) Comparative Example 7 0 0 Example 1 5 0 0 Example 2 3 0 0 Example 3 5 0.5 0 Example 4 5 One 0 Example 5 5 2 0 Example 6 5 One 5 Example 7 5 One 10 Example 8 5 One 15

Major component extraction yield of cucumber (%) Ascorbic acid Caffeic acid Cucurbitacin D 23,24-dihydrocucurbitacin D Comparative Example 100 100 100 100 Example 1 100 100 100 100 Example 2 100 100 100 100 Example 3 110 100 100 100 Example 4 136 112 100 100 Example 5 135 110 100 100 Example 6 170 140 100 100 Example  7 240 210 150 150 Example 8 230 200 140 130

Table 2 shows that the extraction yields of ascorbic acid, caffeic acid, cucurbitacin D and 23,24-dihydrocucurbitacin D were generally higher than those of the extracts of Comparative Examples . Especially, ascorbic acid and caffeic acid were higher than those of Comparative Example by at least 2 times (see Examples 7 to 8), and in particular, they were extracted with Example 7 (pH 5, 1% by weight of sodium chloride and 10% by weight of ethanol) It can be confirmed that the extraction yield is remarkably high.

[Test Example 2] Changes in content of major components of cucumber during storage

The contents of ascorbic acid, caffeic acid, cucurbitacin D and 23,24-dihydrocucurbitacin D in the comparative examples and the examples shown in Table 1 are shown in Tables 3-6 and 1-4 Respectively. The contents of major components were analyzed by centrifuging the extract solution for 10 minutes at 10000RPM for each storage period and injecting the supernatant into high performance liquid chromatography (HPLC). Based on the results of Table 2 above, the content changes in Example 7 and Comparative Example with the highest extraction yield were compared, and the extraction yield in the comparative example was 100.

Changes in content of ascorbic acid by storage period (day) 0 5 10 15 20 Comparative Example 100 50 30 0 0 Example 7 240 296 240 200 160

As shown in Table 3 and FIG. 1, in the comparative example, the content of ascorbic acid was drastically decreased as the storage period passed. Especially, the ascorbic acid was destroyed on the 15th day after storage. However, in Example 7, the content of ascorbic acid gradually decreased as the storage period passed, and the ascorbic acid ratio was maintained at a higher rate than that of the comparative example even after 20 days of storage.

Changes in content of caffeic acid with storage time (day) 0 5 10 15 20 Comparative Example 100 50 40 30 0 Example 7 210 175 175 140 105

As shown in Table 4 and FIG. 2, in the comparative example, the content of caffeic acid was drastically decreased as the storage period passed. Especially, caffeic acid was destroyed at 20 days after storage. However, in Example 7, the caffeic acid content gradually decreased as the storage period passed, and the caffeic acid ratio was maintained at 20 days after the storage as compared with the extraction yield in the comparative example.

Changes in content of cucurbitacin D by storage period (day) 0 5 10 15 20 Comparative Example 100 80 70 70 60 Example 7 150 140 140 140 140

As shown in Table 5 and FIG. 3, in the comparative example, the content of cucurbitacin D drastically decreased as the storage period passed, and cucurbitacin D was almost destroyed at 20 days after storage. In Example 7, It can be confirmed that cucurbitacin D is hardly destroyed.

Changes in the content of 23,24-dihydrocucurbitacin D by storage period (day) 0 5 10 15 20 Comparative Example 100 90 90 80 80 Example 7 150 150 150 150 150

Referring to Table 6 and FIG. 4, it can be seen that the degree of destruction of 23,24-dihydrocucurbitacin D is smaller than that of other components. In particular, in Example 7, it can be confirmed that 23,24-dihydrocucurbitacin D is not destroyed and remains intact.

Claims (13)

A cosmetic composition containing a main ingredient of cucumber extracted using an extractive solution containing a vinegar as a main component, the pH being 3 or more and less than 7. The cosmetic composition according to claim 1, wherein the extraction solution further comprises at least one selected from the group consisting of sodium chloride and an organic solvent. The cucumber as claimed in claim 1, wherein the main component of the cucumber is at least one selected from the group consisting of ascorbic acid, caffeic acid, cucurbitacin D and 23,24-dihydrocucurbitacin D Cosmetic composition. 3. The cosmetic composition according to claim 2, wherein the sodium chloride is contained in an amount of 0 to 1.5% by weight based on the total weight of the extract solution. The cosmetic composition according to claim 2, wherein the organic solvent is contained in an amount of 0 to 20% by weight based on the total weight of the extract solution. The cosmetic composition according to claim 2, wherein the organic solvent is ethanol. A method of extracting a cucumber by extracting a main component of cucumber with an extract solution containing a vinegar as a main component having a pH of 3 or more and less than 7. 8. The cucumber as claimed in claim 7, wherein the main component of the cucumber is at least one member selected from the group consisting of ascorbic acid, caffeic acid, cucurbitacin D and 23,24-dihydrocucurbitacin D Extraction method. 8. The extraction method according to claim 7, wherein the extraction solution further comprises sodium chloride. The extraction method according to claim 9, wherein the sodium chloride is contained in an amount of 0 to 1.5% by weight based on the total weight of the extract solution. 8. The extraction method according to claim 7, wherein the extraction solution further comprises an organic solvent. The extraction method according to claim 11, wherein the organic solvent is contained in an amount of 0 to 20% by weight based on the total weight of the extraction solution. 12. The extraction method according to claim 11, wherein the organic solvent is ethanol.

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