KR20150056306A

KR20150056306A - Transgenic Potato having enhanced drought and salt tolerance and production method thereof

Info

Publication number: KR20150056306A
Application number: KR1020130139159A
Authority: KR
Inventors: 김둘이; 변명옥; 윤인선; 김범기; 이균오; 나종국; 전선아
Original assignee: 대한민국(농촌진흥청장)
Priority date: 2013-11-15
Filing date: 2013-11-15
Publication date: 2015-05-26

Abstract

The present invention relates to transgenic potatos with enhanced salt and drought tolerance and a preparation method thereof. More specifically, the present invention uses the basic-leucine zipper 28 (bZIP28) genes separated from Arabidopsis thaliana and applies the genes for the transformation of the potatoes. Accordingly, the transgenic potatos can have enhanced salt and drought tolerance and be used as disaster-resistant crops capable of being grown normally even under abrupt climate changes due to global warming.

Description

FIELD OF THE INVENTION The present invention relates to a transgenic potato having improved resistance to water,

The present invention relates to a transgenic potato having improved resistance to harshness and salt tolerance by using bZIP28 (basic-leucine zipper 28) gene isolated from Arabidopsis thaliana and a method for producing the same.

Agriculture has unique risks and instabilities that are not found in other industries. Agriculture is not only uncertain because of the large loss of catastrophes as well as large fluctuations in agricultural prices and the relatively low price of agricultural produce compared to the purchase price of production materials. Agriculture is a natural hazard, and it is more vulnerable to damage than any other industry by a pair of floods, frosts, and hail. This is because agricultural production fields and products are closely connected to the land that is impossible to move, and because they are subject to the constraints of life phenomena, they are subject to natural conditions. A pair of crops (drought, dry) causes crop loss due to lack of precipitation or there is no constant precipitation period, so crops are grown depending on irrigation water. A pair of crops can occur at any time during the cultivation period. In the early stage of growth, the flowering time is delayed, and when the long-term flowering is continued, the flowering time is delayed and the yield is seriously affected. Korea has abundant annual precipitation of 1,250 mm, but it is biased in July-August, so it has a semi-dry climate in spring and autumn. Therefore, spring and autumn habitually take a pair, sometimes even in July-August, due to shortage of rainfall (害害).

The salinization of crops occurs when crops are cultivated in salt and sodium soils, and the irrigation of irrigation water with a high salinity level due to the inflow of seawater, the concentration of salts by evaporation of soil water, , And the effect of sea water droplets on storm or wave. In Korea, the three sides are surrounded by the sea, and most of them are composed of coasts, and there are many salt sea areas. The salinity was increased by the intrusion of salt water and the evaporation of soil moisture. As a result, the soil was damaged due to the increase of salt concentration. Some areas were used as a salt field and much of it has been turned into a reclaimed land since the 1960s. Many of them have been matured to increase the yield of rice. However, in the case of a new rice cultivation project which has been completed in recent years, there are many cases where the rice is damaged.

With the development of plant transgenic technology, it became possible to express foreign genes far away from each other. Therefore, introduction of genes resistant to poor environment into susceptible plants has made it possible to develop inherently degradable crops. Therefore, the isolation and development of genes resistant to cold weather, drought, and salting are indispensable requirements for the development of these crops.

Especially, the salty water and the drought cause water shortage due to the osmotic pressure of crops, and the plants cause morphological and metabolic changes. This lack of water causes not only morphological changes of plants but also clogging of pores, reduction of photosynthesis rate and photo respiration rate, increase of small molecules and changes of plant hormones, and changes of gene expression.

As the population grows, food production continues to be demanded, but agricultural and agricultural land is declining. In addition, since the crops are in a stable state, when the surrounding environment changes, there is a great deal of stress during the growing period, resulting in a large difference in yield. Although Korea is in a temperate climate, unusual droughts and high temperature phenomena occur frequently due to global climate change. In the past 10 years (1995-2004), the weather disaster area due to flood, Thousand ha, corresponding to 8.87% of the total land area of 1,836 thousand ha in Korea. Such a decrease in agricultural productivity due to natural disasters is serious, and the development of crops that can withstand these poor environments is absolutely required to increase food production.

Accordingly, the present inventors have searched for a gene for enhancing the drought tolerance and salt tolerance derived from plants, and discovered the Arabidopsis thaliana bZIP28 gene. The bZIP28 gene was transformed into a potato as a main food crop. The potato transformant is a disaster tolerant crop capable of coping with abrupt global climate change due to its improved resistance to abrasion and salt, and is useful for producing crops stably. Will be used.

It is an object of the present invention to provide a method for producing a transformant comprising the bZIP28 gene, which is used for promoting drought tolerance and salt tolerance comprising the nucleotide sequence of SEQ ID NO: 1.

It is another object of the present invention to provide a transgenic plant in which the bZIP28 gene produced by the above-described method is expressed to enhance the resistance to abrasion and salt tolerance.

In order to achieve the above object,

(1) preparing a recombinant vector comprising bZIP28 (basic-leucine zipper 28) gene used for promoting drought tolerance and salt tolerance comprising the nucleotide sequence of SEQ ID NO: 1;

(2) transforming the vector into a plant using Agrobacterium; And

(3) selecting the transformant. The present invention also provides a method for producing a transgenic plant.

In addition, the present invention provides a transgenic plant in which the bZIP28 gene produced by the above-described method is expressed to enhance tolerance and salt resistance.

The plant transformed with the vector containing the bZIP28 gene of the present invention has tolerance to aging and salt tolerance, so it can be used as a disaster tolerant crop which does not cause any problem in productivity even in the case of sudden climate change such as global warming Can be used to make.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic diagram showing a binary vector for potato transformation containing a bZIP28 (basic-leucine zipper 28) gene. FIG.
FIG. 2 is a diagram showing a process for producing a transforming potato through recombinant vector containing bZIP28 gene through Agrobacterium infection.
FIG. 3 is a graph showing the results of confirming the expression analysis after treatment with bZIP28 gene-transforming potatoes at 23 ° C. and 45 ° C. (high temperature).
FIG. 4 shows the results of treatment of 10% PEG in the bZIP28 transgenic potatoes 1 (Z1), 6 (Z6), 8 (Z8) and 9 (Z9) lines and control (Sumi) for 0, 3, 6, The results are shown in FIG.
5 is a graph showing the results of measurement of the activity of the bZIP28 transgenic potato 1 (Z1), 6 (Z6), 8 (Z8) and 9 (Z9) lines and 250 mM sodium chloride (NaCl) at 0, 3, 6, 12 and 24 FIG. 5 is a graph showing the results of analysis of expression after time treatment. FIG.
FIG. 6 is a diagram showing the growth state after 3 weeks treatment with 0.3% PEG in bZIP28 gene transformed potato 3 (Z3), 6 (Z6) and 8 (Z8) lines and control group (Sumi).
FIG. 7 is a graph showing the growth state after treatment with 0.3% PEG for 7, 14 and 21 days in the bZIP28 transgenic potato 3 (Z3), 6 (Z6) and 8 (Z8) lines and the control group (Sumi).
FIG. 8 is a diagram showing the growth state after 3 weeks treatment with 75 mM NaCl (NaCl) in bZIP28 transgenic potato 3 (Z3), 6 (Z6) and 8 (Z8) lines and control group (Sumi).
9 is a graph showing the growth state after treatment of 75 mM sodium chloride (NaCl) for 7, 14 and 21 days in bZIP28 transgenic potato 3 (Z3), 6 (Z6) and 8 (Z8) lines and control to be.
FIG. 10 is a graph showing the growth state after treatment with bZIP28 gene-transforming potato 3 (Z3), 6 (Z6) and 8 (Z8) lines and control group (Sumi).
FIG. 11 is a diagram showing the growth state after treatment with 250 mM sodium chloride (NaCl) for 6 days in bZIP28 transgenic potato 3 (Z3), 6 (Z6) and 8 (Z8) lines and control group (Sumi).

Hereinafter, the present invention will be described in detail.

The present invention

(1) preparing a recombinant vector comprising bZIP28 (basic-leucine zipper 28) gene which is used for drought tolerance and salt resistance enhancement consisting of the nucleotide sequence of SEQ ID NO: 1;

(2) transforming the vector into a plant using Agrobacterium; And

The gene is preferably isolated from Arabidopsis thaliana L., but is not limited thereto.

Variants of the above base sequences are also included within the scope of the present invention. Specifically, the gene has a nucleotide sequence having a sequence homology of 70% or more, more preferably 80% or more, still more preferably 90% or more, and most preferably 95% or more, with the nucleotide sequence of SEQ ID NO: 1 . "% Of sequence homology to polynucleotides" is ascertained by comparing the comparison region with two optimally aligned sequences, and a portion of the polynucleotide sequence in the comparison region is the reference sequence for the optimal alignment of the two sequences (I. E., A gap) relative to the < / RTI >

The recombinant vector preferably includes but is not limited to a BIP3P promoter.

The recombinant vector is preferably, but not limited to, for enhancing resistance to aridity and salt tolerance.

The recombinant vector is prepared by attaching the bZIP28 gene according to the present invention to a BIP3P promoter, and may be the vector described in FIG. 1, but is not limited thereto.

The term "recombinant" refers to a cell in which a cell replicates a heterologous nucleic acid, expresses the nucleic acid, or expresses a protein encoded by a peptide, heterologous peptide or heterologous nucleic acid. The recombinant cell can express a gene or a gene fragment that is not found in the natural form of the cell in one of the sense or antisense form. In addition, the recombinant cell can express a gene found in a cell in its natural state, but the gene has been modified and reintroduced intracellularly by an artificial means.

The term "vector" is used to refer to a DNA fragment (s), nucleic acid molecule, which is transferred into a cell. The vector replicates the DNA and can be independently regenerated in the host cell. The term "expression vector" is often used interchangeably with a "recombinant vector ". The term "recombinant vector" means a recombinant DNA molecule comprising a desired coding sequence and a suitable nucleic acid sequence necessary for expressing a coding sequence operably linked in a particular host organism. Promoters, enhancers, termination signals and polyadenylation signals available in eukaryotic cells are known.

The vector of the present invention can typically be constructed as a vector for cloning or expression. In addition, the vector of the present invention can be constructed by using prokaryotic cells or eukaryotic cells as hosts. For example, when the recombinant vector of the present invention is an expression vector and a prokaryotic cell is used as a host, a strong promoter (for example, pL promoter, trp promoter, lac promoter, T7 promoter, tac promoter, etc.) , Ribosome binding sites for initiation of detoxification, and transcription / translation termination sequences.

The vectors that can be used in the present invention include plasmids such as pSC101, ColE1, pBR322, pUC8 / 9, pHC79, pGEX series, pET series and pUC19, z1 and M13) or a virus (e.g., SV40 or the like).

On the other hand, when the recombinant vector of the present invention is an expression vector and a eukaryotic cell is used as a host, a promoter derived from the genome of a mammalian cell (e.g., a metallothionein promoter) or a mammalian virus (e.g., Adenovirus late promoter, vaccinia virus 7.5K promoter, SV40 promoter, cytomegalovirus promoter, and tk promoter of HSV) can be used, and generally have a polyadenylation sequence as a transcription termination sequence.

The vector of the present invention may be a selection marker and may include an antibiotic resistance gene commonly used in the art, for example, ampicillin, gentamycin, carbenicillin, chloramphenicol, streptomycin, kanamycin, , Tetracycline and basta herbicides.

In addition, the present invention provides a transformant transformed with said recombinant vector.

The plant is preferably, but not limited to, a plant having increased resistance to harshness and salt by expression of bZIP28 gene.

The plant of step 2) is preferably at least one selected from the group consisting of potato, rice, cabbage, cabbage, mustard, rapeseed, radish, Brassica napobrassica and Brassicarapa / Brassica campestris, Is most preferable.

The plant is preferably at least one selected from the group consisting of potato, rice, cabbage, cabbage, mustard, rapeseed, radish, Brassica napobrassica and Brassicarapa / Brassica campestris, Do.

In the case of Arabidopsis, in the case of Arabidopsis, it is possible to easily transform the Arabidopsis thaliana in a comparatively short time by transforming the Agrobacterium into an artificial immature embryo before flower modification by the flower dipping method, This saves a lot. However, Arabidopsis is only used as a model plant, and it is very difficult to see that it can be applied to actual crops.

There are not many transgenic crops in cultivated farms. In the case of potatoes, a transgenic method has been developed. However, by using the leaves or stems of transgenic plants for transformation, It is highly likely that the mutants will come out, so that it is difficult to obtain a transformant.

In the present invention, transgenic potatoes transformed with a vector containing the bZIP28 (basic-leucine zipper28) gene were constructed to overcome the above difficulties. This is because not only a transformant in which a useful gene is inserted is obtained by using the characteristics of potatoes capable of nutrition and propagation in a tuber, but also the genetic property can be stably maintained in the next generation since the transformant immediately becomes a fixed line And potatoes transformed with the bZIP28 gene can be used as a disaster tolerant crop because they are resistant to weathering and salt tolerance.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are intended to illustrate the contents of the present invention, but the scope of the present invention is not limited to the following examples. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art.

< Example 1> bZIP28 Gene isolation

For the isolation of the bZIP28 gene, First, total RNA was extracted from Arabidopsis leaf and first strand cDNA was synthesized using sprint RT complete oligodT (Clontech).

Specifically, a 5'-ATGACGGAATCAACATCCGTG-3 '(SEQ ID NO: 2) and an antisense primer 5'-TCTAGAGGTGGCTACGAGATGGAGACCC-3' (SEQ ID NO: 3) were synthesized as a primer for amplifying the fragment. The first strand cDNA of potato was used as a template and PCR amplified with Takara's Ex Taq polymerase was amplified using Applied Biosystem 9700. The PCR reaction conditions used were denaturation at 95 ° C for 10 min, followed by 25 cycles of 94 ° C for 1 min, 56 ° C for 1 min, and 72 ° C for 2 min, followed by treatment at 72 ° C for 10 min. After completion of the PCR reaction, the amplified gene was electrophoresed on 1% agarose, and the bands corresponding to the bZIP28 gene were cut out, purified using a QIAquick gel extraction kit, and then used for cloning. The obtained bZIP28 anticipated gene was cloned into a Topo 2.1 vector (Invitrogen, TOPO TA Cloning kit) and plated on LB medium supplemented with X-gal. White colonies obtained were selected and subjected to colony PCR.

As a result, the plasmid of the identified bands was extracted and confirmed to be the bZIP28 gene using a sequencer ABI 3700 sequencer. As a result of the base sequence analysis, bZIP28 gene was obtained and shown in SEQ ID NO: 1.

< Example 2> bZIP28 Generation of transgenic vectors containing genes

The inventors constructed vectors to transform bZIP28 gene into potato.

Specifically, the bZIP28 gene isolated in Example 1 was specifically inserted into the inducible promoter BIP3P by linking the bZIP28 gene to BIP3P: bZIP28 in the pGreen0049 vector, which is a binary vector for transformation into potato, for potato transformation (Fig. 1).

< Example 3> bZIP28 Production of transformants containing genes

Transgenic plants were prepared using Agrobacterium as the potato transformation vector prepared in Example 2 above.

Specifically, in order to transform a binary vector into which bZIP28 gene has been inserted into Agrobacterium (LBA4404), freezing and thawing were repeated 2 to 3 times, followed by heat shock at 37 DEG C After transformation, colonies were identified in YEP medium (Yeast 10 g, NaCl 5 g, peptone 10 g, Agar 15 g / L) overnight. Colonies confirmed to be transformed by colony PCR were transformed into AB medium (AB buffer (K ₂ HPO ₄ 60 g, NaH ₂ PO ₄ 20 g / L), AB Salts (NH ₄ Cl 60 g, MgSO ₄ 7H ₂ O 6g, KCl 3g, CaCl ₂ 2H ₂ O 0.265g, FeSO ₄ .7H ₂ O 50mg / 1L) and glucose 5g / L).

Then, as shown in FIG. 2, to obtain potato transformants, the Agrobacterium-transformed genes cultured in the potato seedlings and the YEP medium cultured in the MS medium for 2 days were incubated with MS liquid medium for 30 minutes The cells were transferred to MS solid medium to which cefotaxime and hygromycin were added and cultured for 7 days. The cells were transferred to callus induction medium for 7 days, then cultured in fresh MS medium supplemented with cefotaxime and hygromycin for 7 days. For the induction of shooting, the mice were transferred to a new medium every 2 weeks and repeatedly transplanted two times. After shooting, they were subcultured to obtain an overexpressed transformant having the bZIP28 gene inserted therein.

< Example 4> bZIP28 Selection of Transgenic Plants Containing Genes

The present inventors obtained 20 lines of the overexpressed potato transformants inserted with the bZIP28 gene prepared in Example 3, but all of the lines having no phenotypic or anomalous gene were removed, and 11 lines Were selected.

< Experimental Example 1> High-temperature treated transformants bZIP28 Gene expression analysis

The transformed potato comprising the bZIP28 gene selected in Example 4 was subjected to high temperature treatment To analyze the expression of bZIP28 gene, The expression of bZIP28 gene was analyzed by RT-PCR.

Specifically, in order to analyze resistance to high-temperature stress in the culture chamber, bZIP28 transformant was treated for 1 hour in a dryer at 45 ° C, total RNA was extracted from the leaves of bZIP28 transformed potatoes after 6 hours at room temperature and Sprint RT Complete First strand cDNA was synthesized using-OligodT (Clontech).

A primer 5'-ATGACGGAATCAACATCCGTG-3 '(SEQ ID NO: 2) and an antisense primer 5'-TCTAGAGGTGGCTACGAGATGGAGACCC-3' (SEQ ID NO: 3) were synthesized as a primer for amplifying the fragment, The first strand cDNA was used as a template and PCR amplified with Takara's Ex Taq polymerase was amplified using Applied Biosystem 9700. The PCR reaction conditions used were denaturation at 95 ° C for 10 min, followed by 25 cycles of 94 ° C for 1 min, 56 ° C for 1 min, and 72 ° C for 2 min, followed by treatment at 72 ° C for 10 min. After completion of the PCR reaction, the amplified gene was electrophoresed on 1% agarose to confirm the expression level.

As a result, the expression of bZIP28 gene was increased in most transgenic potatoes. Especially, the expression of bZIP28 gene in line 3 (Z3), 6 (Z6), 7 (Z7), 8 (Z8) Fold increase (Fig. 3).

< Experimental Example 2> Osmotics Tres ( PEG ) Treated transformants bZIP28 Gene expression analysis

The transformed potatoes containing the bZIP28 gene selected in Example 4 To analyze the expression of bZIP28 gene, PEG The expression of bZIP28 gene was analyzed by RT-PCR.

Specifically, 10% PEG was added to the bZIP28 transgenic potatoes 1 (Z1), 6 (Z6), 8 (Z8) and 9 (Z9) lines and the control Time processing. Then, the first template cDNA was synthesized by using Sprint RT Complete-OligodT (Clontech), and then the primer for amplifying the fragment was used as a sense primer 5'-ATGACGGAATCAACATCCGTG-3 (SEQ ID NO: 2) and an antisense primer 5'-TCTAGAGGTGGCTACGAGATGGAGACCC-3 '(SEQ ID NO: 3) were subjected to RT-PCR in a PCR reaction solution and then electrophoresed on 1% agarose to obtain bZIP28 Expression.

As a result, the expression level of bZIP28 gene was highest in the bZIP28 transgenic potato compared to the wild type after 3 hours of 10% PEG treatment, and gradually decreased until 24 hours thereafter (FIG. 4).

< Experimental Example 3 > salt treated transformants bZIP28 Gene expression analysis

The transformation potatoes containing the bZIP28 gene selected in the above example were subjected to salt treatment To analyze the expression of bZIP28 gene, NaCl The expression of bZIP28 gene was analyzed by RT-PCR.

Specifically, 250 mM NaCl was added to the bZIP28 transgenic potatoes 1 (Z1), 6 (Z6), 8 (Z8) and 9 (Z9) lines and the control group Time processing. The expression level was confirmed by the method of Experimental Example 2 above.

As a result, the bZIP28 gene was expressed in bZIP28 transgenic potato lines 1 (Z1), 6 (Z6), 8 (Z8) and 9 (Z9) Sumi), it was confirmed that the bZIP28 gene was not expressed (Fig. 5).

< Experimental Example 4> bZIP28 Of the transformant containing the gene Osmotic Stress analysis

To test for osmotic stress in the transformed potatoes 3 (Z3), 6 (Z6) and 8 (Z8) containing the bZIP28 gene selected in the above example, the transformant in which the bZIP28 gene was inserted was cultured in an aseptic culture room The osmotic stress response of each line was examined after treatment with MS solid medium supplemented with 0.3% PEG for 7 days, 14 days and 21 days.

As a result, it was confirmed that the transgenic potatoes 3 (Z3), 6 (Z6) and 8 (Z8) lines containing the bZIP28 gene were superior to those of the control (Sumi) in growth and root development (FIGS. 6 and 7)

< Experimental Example 5> bZIP28 Salt resistance analysis of transformants containing genes

Transgenic potatoes containing the bZIP28 gene selected in the above examples were treated with salts and analyzed for their growth.

Specifically, transgenic potato lines 3 (Z3), 6 (Z6) and 8 (Z8) lines containing the bZIP28 gene selected in the examples were cultured in MS solid medium supplemented with 75 mM NaCl in an aseptic culture incubator for 7 days, After 14 and 21 days treatment, the salt tolerance was examined.

As a result, it was confirmed that the transgenic potatoes 3 (Z3), 6 (Z6) and 8 (Z8) lines containing the bZIP28 gene were superior to those of the control (Sumi) in growth and root development (FIGS. 8 and 9)

< Experimental Example 6> bZIP28 Of the gene transformant Dry Enhancement analysis

The transgenic potatoes containing the bZIP28 gene selected in the above example were measured for drought tolerance after dry stress treatment.

Specifically, the transgenic potatoes 3 (Z3), 6 (Z6) and 8 (Z8) lines containing the bZIP28 gene and the control group (Sumi) were grown in a greenhouse at 30 ° C to 35 ° C for 3 weeks, And subjected to dry stress treatment.

As a result, the dry state of the transformant of the control group (sumi) began to change from about 6 days after the start of the drying treatment, and the transformant having the bZIP28 gene inserted therein was about 3 ~ And maintained a healthy growth state for 5 days.

In addition, after the drying stress, the bZIP28 gene transfected with the bZIP28 gene was regenerated gradually from day 3 after the treatment until immediately before the test, and the growth of the transformant with the bZIP28 gene was clearly differentiated from the control group (Fig. 10).

< Experimental Example 7> bZIP28 Analysis of Increase in Salt Tolerance of Gene Transformants

The transformed potatoes containing the bZIP28 gene selected in the above example were measured for salt tolerance after salt stress treatment.

Specifically, the transformed potatoes 3 (Z3), 6 (Z6) and 8 (Z8) lines containing the bZIP28 gene and the control group (Sumi) were treated with 250 mM NaCl for 2 days, The phenotype was confirmed after feeding.

As a result, it was confirmed that the salt was stronger than the transformed potatoes 3 (Z3), 6 (Z6) and 8 (Z8) lines containing the bZIP28 gene and the control group (Sumi).

<110> RURAL DEVELOPMENT ADMINISTRATION <120> Transgenic Potato with enhanced drought and salt tolerance and production method thereof <130> P131049 <160> 3 <170> Kopatentin 2.0 <210> 1 <211> 2028 <212> DNA <213> Arabidopsis thaliana L. <400> 1 atgacggaat caacatccgt ggttgctcct ccgccggaga tacctaatct gaaccctagc 60 atgttttctg agtccgattt gttttctatt ccgccgctag atcctctttt cctatctgat 120 tctgatccga tttcaatgga tgcgccaatc tccgatctcg acttcttact cgacgatgag 180 aacggagatt tcgctgattt tgatttctcg tttgataatt ctgatgattt cttcgatttc 240 gatttatcgg agcccgcggt ggtgatccct gaggagatcg gtaacaatcg ttcgaatttg 300 gactcatcgg aaaacagaag cggcgatgga ggtttagaag gaagatctga gtctgttcat 360 tcacaggttt catctcaagg ctccaagact tttgtgtccg acaccgttga cgcatcatcc 420 tcccctgaat caagcaatca ccagaaatct tctgttagca agaggaagaa ggaaaatgga 480 gactccagtg gcgaattaag gagctgcaag taccaaaagt ccgatgataa atcagtcgct 540 acgaacaacg aaggtgatga tgacgacgac aagaggaagt tgataaggca gattaggaac 600 cgtgaaagtg ctcagctttc gaggttgagg aagaagcaac aaactgagga gcttgaaaga 660 aaagtgaaga gtatgaatgc taccattgct gaattgaatg gtaagattgc ttatgttatg 720 gctgagaatg tcgctttaag gcaacaaatg gctgttgctt ctggtgctcc tcctatgaat 780 ccttatatgg ctgccccgcc tttaccgtat caatggatgc cgtatccgcc gtatcctgtt 840 aggggatatg gatcacagac acctttggtt cccattccta agttaaatcc taagcctgtc 900 tcgagttgta gaccgaagaa ggcagagagt aagaagaatg agggtaaaag taagctcaag 960 aaggttgcta gtattagttt tattggaatt ctcttctttg tcttcttgtt tggtacattg 1020 gttcctttta tgaatgtaaa ttttggagga gaacgtggaa gctttggcgg tttgtctaaa 1080 tatgatggcc accggtatta cgatgaacat aaggggaggg ttttaatggt cggcgatggt 1140 tctgatgtta gaagaaatag tggaatttct gaaggaaata tccattctag taggattagt 1200 catggtgaga gagatagttg tggaggagta gattataatg ctcatccgaa agtagaagga 1260 cgaccaagtt cgttgagcaa tgccagtgat cctctctttg cttctctcta tgtcccaaga 1320 aacgatgggc ttgtgaagat cgatgggaac ttgataattc actctgtttt ggcgagcgag 1380 aaagcaaggg gtttaggaaa gaagaatatc actgaaacag taaaaactaa agaaccggat 1440 ttgaccattc ctggtgcact gtcttctgca ttagctgttc cgggggtaag aggaaatgca 1500 gcaatgcttc cgcattcaac agctctctct tctgaaggga aaagacttca ccaatggttt 1560 catgaaggtg gctcagggcc actaatggat tacagcatgt gcaccgaggt tttccagttt 1620 gatattgctc ctggtgctat agtcccgtca tcagtctcca gcatttctgc ggagcatctc 1680 caaaatgtca ctacccacgg caagagaatg aagaacagga gaatccttga gggacttcct 1740 gtttcacttg tggcgtcaga gctcaatatc accggaaccc agccaaacaa agacgctcaa 1800 aataagacct ttaatggaaa cactaacaaa cccacatcat catcctccat ggttgtctca 1860 gtgttacttg atccaagaga ggtcgttgac tctgaaaccg acagagtggt tcccccaaac 1920 ccaaaatcac tttcccggat ctttgtggtg gtgcttcttg acagtgtcaa gtacgttacc 1980 tactcatgcg ttcttcctcg atcgggtctc catctcgtag ccacctga 2028 <210> 2 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> bZIP28 sense primer <400> 2 atgacggaat caacatccgt g 21 <210> 3 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> bZIP28 antisense primer <400> 3 tctagaggtg gctacgagat ggagaccc 28

Claims

(1) preparing a recombinant vector comprising bZIP28 (basic-leucine zipper 28) gene used for promoting drought tolerance and salt tolerance comprising the nucleotide sequence of SEQ ID NO: 1;
(2) transforming the vector into a plant using Agrobacterium; And
(3) selecting the transformant.

The method according to claim 1, wherein the bZIP28 gene is isolated from Arabidopsis thaliana L.

2. The method of claim 1, wherein the recombinant vector comprises a BIP3P promoter.

The plant according to claim 1, wherein the plant of step 2) is selected from the group consisting of potato, rice, cabbage, cabbage, mustard, rapeseed, Brassica napobrassica and Brassicarapa / Brassica campestris. Or more of the transgenic plant.

The method according to claim 1, wherein the plant is characterized in that the bzIP28 gene expression increases the tolerance and salt resistance.

A transgenic plant in which the bZIP28 gene produced by the manufacturing method of claim 1 has been improved in the resistance to harshness and salt tolerance.

The plant according to claim 6, wherein the plant is at least one selected from the group consisting of potato, rice, cabbage, cabbage, mustard, rapeseed, radish, Brassica napobrassica and Brassicarapa / Brassica campestris Lt; / RTI >