KR20150031562A - C2-5 chemical producing acetogens and Production conditions using synthesis gas - Google Patents

Abstract

The present invention relates to a strain, which uses a synthesis gas as a carbon source and produces an organic acid as a by-product. According to the present invention, an oscillibactersp._whose accession number is KCTC 12249BP uses a synthesis gas as a matrix and accordingly produces C5 of isovaleric acid, compared with an existing acetogen for producing C2 or C4 of carbon compounds, thereby expanding the range of producing biochemical compounds.

Description

[0001] The present invention relates to a method for producing a carbonaceous compound using a syngas and a method for producing the same,

The present invention relates to a novel strains belonging to the genus Oshilacteria which can produce a multifunctional carbon compound having 2 to 5 carbon atoms using a syngas derived from a biomass as a substrate, which is a gaseous substrate, and a multifunctional carbon compound having 2 to 5 carbon atoms .

Synthetic gas is generated when the organic raw material is partially oxidized under the condition of high temperature steam. It mainly consists of CO, H _2, and CO ₂ , and raw materials include natural gas, coal, oil shale, (tar sand) can be used, and they are mainly produced by metal catalytic reaction of natural gas or coal.

The purity of syngas (using only CO and H ₂ ) is very important in the conversion of syngas to a multicomponent compound using a metal catalyst. When impurities are contained in the raw material, the reaction rate is greatly reduced and the productivity is lowered. In particular, when a sulfur compound is contained as an impurity, the sulfur compound is irreversibly poisoned by the metal catalyst, and it is difficult to reactivate the catalyst, and a separate manufacturing process is required to remove the sulfur compound, and it is difficult to concentrate CO or H ₂ And it is not economical.

Recently, a technology to produce syngas by vaporizing biomass has been developed. The process of using biomass can lower the concentration of sulfur compounds contained in syngas or use it with lower energy than the case of using coal, Coal: 1500-1800 占 폚, biomass: 1100 占 폚) at a relatively short reaction time.

Acethgens is a microorganism that can utilize such syngas derived from biomass. These microorganisms, which are defined as Homoacetogen, are anaerobic microorganisms which grow CO or H ₂ as a unique energy source through the Wood-Ljungdahl pathway. Acethyl-CoA synthease (or carbon monoxide dehydrogenase , Hereinafter referred to as CODH), which is involved in the oxidation of CO and the reduction of CO ₂ . The use of the wood-drag route allows syngas to be converted to organic acids without additional purification or concentration processes, and is carried out at room temperature and atmospheric pressure, and is not affected by sulfur impurities. Bacteria known to ferment synthetic gas to date include Clostridium ljungdahlii (Enzyme and Microbial Technology 14 (8), 1992 602-608 .; Applied Biochemistry and Biotechnology 45 (6), 1994 145-157 .; Biochemical Engineering Journal 27, 2005 110-119), Clostridium autoethanogenum (Archives of Microbiology 161 (4), 1994345-351.), Clostridium carboxidivorans P7 (Biotechnology and Bioengineering 97 (5), 2007 1080-1086 .; Biomass and Bioenergy 23 2002 487-493.) And Clostridium ragsdalei P11 (US Patent No. 7,704,723).

On the other hand, isovaleric acid having five carbon atoms is used as a perfume, a pharmaceutical composition, and an organic acid used for various pathological studies. It is used in the fields of Valeriana, Origanum majorana, Hop and animal lipids But it has a problem in that it is difficult to raise the oil because of low profitability. Korean Patent No. 0372218 relates to a method for producing organic acids by cultivating Mannheimia sp. 55E and an electrophilic strain under anaerobic or aerobic conditions. More particularly, it relates to a method for producing succinic acid, lactic acid, and formic acid under anaerobic conditions saturated with carbon dioxide U.S. Patent Application Publication No. 2008/0311640 discloses that hydrogen and organic acids are produced using various microorganisms. However, the above-mentioned prior arts disclose that acetyl, CoA, And four individual organic acids. Therefore, it is time to study the strains which have expanded the production of biological compounds so as to produce organic acids having 2 to 5 carbon atoms, especially isovaleric acid.

The first problem to be solved by the present invention is to use a syngas derived from biomass, which is a gaseous substrate, to produce a carbonaceous compound having 2 to 5 carbon atoms, particularly a strain having the characteristic of producing isovaleric acid having 5 carbon atoms .

A second problem to be solved by the present invention is to provide a method for producing a carbon compound having carbon atoms of 2 to 5 using carbon monoxide as a substrate using the strain according to the present invention.

To achieve the above object, the present invention is o-silico bakteo in (Oscillibacter isolated from bovine anger having a characteristic to produce the carbon compound is a carbon number of 2 to 5 sp.) C5 (Accession No .: KCTC 12249BP).

According to an embodiment of the present invention, the Oscillator sp.) C5 [Accession No .: KCTC 12249BP] can produce carbon-carbon compounds having 2 to 5 carbon atoms using a carbon monoxide gas source as a substrate. The carbon monoxide gas source may be a syngas.

According to an embodiment of the present invention, the carbon black compound having 2 to 5 carbon atoms is selected from the group consisting of acetic acid, propionic acid, butyric acid, isobutyric acid, ethanol, propanol, butanol, isobutanol, pentanol and isovaleric acid , Especially a polycarbonate compound containing isovaleric acid having 5 carbon atoms.

According to another embodiment of the present invention, the Oscillator sp.) C5 [Accession No .: KCTC 12249BP] may be an anaerobic microorganism,

It may have a gene encoding enzymes involved in the Wood-Ljungdahl pathway,

Genes coding for the enzymes involved in the wood-mediated pathway include acetyl coA synthase, carbon monoxide dehydrogenase, formyltetrahydrofolate synthase, A formimidotetrahydrofolate cyclodeaminase, a formyltetrahydrofolate cyclohydrolase / methylenetetrahydrofolatedhehydrogenase, and a methylene tetrahydrofolate reductase, such as methylene tetrahydrofolate reductase), but the present invention is not limited thereto.

According to another aspect of the present invention, And Oscillibacter (KCTC 12249BP), and culturing the transformed plant cell line. The method for producing the carbon nanofibers having the carbon number of 2 to 5 is also provided.

The culture may be carried out under anaerobic conditions,

More specifically, by carrying out shaking culture at a pH of 5.5 to 8.0 and a temperature of 25 to 50 DEG C at a rate of 50 to 500 rpm for 12 to 72 hours, a multi-carbon compound having 2 to 5 carbon atoms is prepared .

According to another embodiment of the present invention, after the cultivation is completed, the method may further include extracting the carbon compound having 2 to 5 carbon atoms using an organic solvent,

The organic solvent may be at least one selected from the group consisting of dichloromethane, diethyl ether, ethyl acetate, tetrahydrofuran, N, N-dimethylformamide, toluene and chloroform.

The carbon compound having 2 to 5 carbon atoms prepared according to the present invention may be at least one selected from the group consisting of acetic acid, propionic acid, butyric acid, isobutyric acid, ethanol, propanol, butanol, isobutanol, pentanol and isovaleric acid In particular, can be prepared containing isovaleric acid with five carbon atoms.

Oscillibacter sp. C5 (Accession No .: KCTC 12249BP), a novel strain belonging to the genus Oscillospiraaceae according to the present invention, is isolated from cattle manure, It is possible to produce carbon compounds, especially isobaric acid having 5 carbon atoms. Oscillibacter sp. C5 according to the present invention has a carbon number of 5 or more, which is different from the conventional acetogen, which produces only carbon compounds having 2 and 4 carbon atoms, using synthesis gas as a carbon source. Individual carbon compounds could also be produced, thus expanding the production range of biological compounds.

1 is a schematic view of a gas levitation reactor according to an embodiment of the present invention. FIG. 1A is an overall schematic diagram of a system used for microbial enrichment culture, and FIG. 5B is a specific schematic diagram of a gas levitation reactor.
FIG. 2 is a result of forming a single colony using the Hungate tube technique after the concentration cultivation according to an embodiment of the present invention.
FIG. 3 is an evolutionary diagram of C5 in acyclovir prepared by homology analysis according to the present invention.
4 is an image obtained by scanning an Oscillibacter sp. C5 [Deposit No. KCTC 12249BP] according to the present invention with a Scanning Electron Microscope (SEM). 4A is an image in the form of a cluster, and FIG.
FIG. 5 shows the analysis of an organic acid prepared by Oscillibacter sp. C5 (Accession No .: KCTC 12249BP) according to the present invention by a gas chromatography mass spectrometer. FIG. 5A is a gas chromatography mass spectrometry result of an organic acid prepared using a syngas, and FIG. 5B is a gas chromatographic mass spectrometry result of a comparative example of isovaleric acid.
FIG. 6 is a graph showing the results of analysis of an organic acid prepared by Oscillibacter sp. C5 (Accession No .: KCTC 12249BP) according to the present invention using a gas chromatograph mass spectrometer and comparing the peak of isovaleric acid with the internal library .

All technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is well known and commonly used in the art to which the present invention pertains.

In general, microorganisms that grow using biomass-derived syngas (such as CO, H _2, and CO ₂ ) as a reducing power and carbon source and produce organic materials as by-products are named as Acetogen, (COD synthetase) or carbon monoxide dehydrogenase (CODH), which is a bifunctional enzyme, on the metabolic pathway, which is an anaerobic microorganism that grows through the Wood-Ljungdahl pathway. . The carbon monoxide dehydrogenase produces acetic acid, butyric acid, ethanol and butanol as a key element involved in the oxidation of carbon monoxide and the reduction of carbon dioxide. Usually, carbon monoxide is oxidized to carbon dioxide by a carbon monoxide dehydrogenase, Is reduced to acetyl coenzyme A (acethyl-CoA), which has two hydroxyl groups. At this time, the synthesized acetyl coenzyme A is converted into acetic acid and ethanol, and two acetyl coenzyme A binds to convert to acetoacetyl coenzyme A (Acetoacetyl-CoA) having four carbons, which is converted into butyric acid and butanol. Conventional acetogens can only produce organic acids having two or four carbon atoms as described above, and studies on strains producing organic acids having an odd number of carbon atoms (organic acids having 3 or 5 carbon atoms) are in short supply. Therefore, the present inventors have made intensive efforts to find a strain that can produce an organic acid having an odd number of carbon atoms, particularly, isovaleric acid having 5 carbon atoms. As a result, the novel strain Oscillibacter sp. C5 [Deposit number: KCTC 12249BP], thereby completing the present invention.

Hereinafter, the present invention will be described in more detail.

The strain according to the present invention can produce an organic acid containing 2 to 5 carbon atoms, especially an organic acid having 3 or 5 carbon atoms, using carbon monoxide (C1) derived from biomass, which is a gaseous substrate.

The strain according to the present invention was obtained by comparing and analyzing the homology of 16S ribosomal DNA (16S rDNA) obtained from the strain, and found that the Oscillospiraceae family ), and the closest strain, Oscillibacter valericigenes ) and 98% homology. However, it was confirmed that the above-mentioned E. coli Valeriensis is not a strain using carbon monoxide, and based on this, the selected strains were found to contain carbon monoxide It was confirmed that it belongs to an undiscovered genus. It was named as Oscillibacter sp. C5 and was deposited with the KSCC (KCTC, Korea Science and Engineering Foundation, Daejeon Metropolitan City, Korea) KCTC 12249BP, deposited on July 23, 2012 as the deposit number.

According to the present invention, the multi-carbon compound having 2 to 5 carbon atoms is at least one selected from the group consisting of acetic acid, propionic acid, butyric acid, isobutyric acid, ethanol, propanol, butanol, isobutanol, pentanol and isovaleric acid In particular, it may include a multi-carbon compound having 3 or 5 carbon atoms such as propionic acid, propanol and isovaleric acid.

The silica according to the present invention five bakteo in (Oscillibacter sp.) C5 [Accession No .: KCTC 12249BP] can have a gene coding for enzymes involved in the Wood-Ljungdahl pathway, using a carbon monoxide carbon source as a substrate to produce carbon compounds with 2 or 4 carbon atoms And genes coding for the enzymes involved in the wood-mediated pathway are acetyl coA synthase, carbon monoxide dehydrogenase, formate tetrahydrofolate synthase Formyltetrahydrofolate cyclohydrogenase, formyltetrahydrofolate cyclohexanase, formyltetrahydrofolate cyclohydrolase / methylene tetrahydrofolate dehydrogenase, and the like. Methylene tetrahydrofolate reductase tetrahydrofolate reductase), but the present invention is not limited thereto.

The carbon monoxide carbon source may be a synthesis gas, and the synthesis gas may be any one selected from the group consisting of CO, H _2, and CO ₂ . When carbon monoxide is used as a carbon source, carbon monoxide is oxidized to carbon dioxide by a carbon monoxide dehydrogenase, and organic acids are produced through a wood-droplet pathway by using generated electrons and carbon dioxide. When carbon dioxide is used, H ₂ And it is possible to generate organic acids through the same route.

According to the present invention, the isovaleric acid having 5 carbon atoms can be prepared by the following mechanism. Acetyl coenzyme A was produced through the wood-eluting pathway in the metabolic pathway using carbon monoxide as a substrate, and KEGG using 2-isopropyl maleate hydrolase, 3-isopropyl maleate dihydratase and dihydrogenase L-leucine is produced through the Kyoto Encyclopedia of Genes and Genomes pathway and then converted to isovaleryl-CoA by alpha-keto acid dehydrogenase, and is transformed into isovaleryl phosphate using phosphotransacylase After conversion, isovaleric acid can be generated by an acyl kinase.

In five bakteo silica according to the invention (Oscillibacter C5 [Deposit number: KCTC 12249BP] is a rod bacterium of absolute anaerobic and non-flowability, and growth is observed in the range of 25 to 50 ° C, but the preferred growth temperature is 32 to 40 ° C, The temperature is 35-38 ° C. The growth is observed in the range of pH 5.5 to 8.0, but the preferred growth pH is in the range of 6 to 7.5, and the pH is more preferably in the range of 6.5 to 7.2.

The present invention relates to a method for producing carbon monoxide; And five bakteo in Sicily (Oscillibacter (KCTC 12249BP), and culturing the transformed plant cell line. The method for producing the carbon nanofibers having the carbon number of 2 to 5 is also provided.

In five bakteo silica according to the invention (Oscillibacter C5 [Accession No .: KCTC 12249BP] is an absolute anaerobic strain, and the cultivation should be carried out under anaerobic conditions, and at a pH of 5.5 to 8.0 and at 25 to 50 ° C at a rate of 50 to 500 rpm, For a period of time ranging from 100 to 300 rpm for 12 to 72 hours at a pH in the range of from 6 to 7.5 and a temperature in the range of from 32 to 40 < RTI ID = 0.0 > Followed by culturing with shaking.

After the completion of the culturing, the organic solvent may be extracted with an organic solvent, and the organic solvent may include dichloromethane, diethyl ether, ethyl acetate, tetrahydrofuran, N, N-dimethylformamide, toluene and chloroform.

Hereinafter, the present invention will be described in more detail with reference to preferred embodiments. It will be apparent, however, to those skilled in the art that these embodiments are for further explanation of the present invention and that the scope of the present invention is not limited thereby.

Example 1: Synthesis of Oscillibacter sp.) Isolation of C5

Example 1.1. Isolation and Culture of Strain from Cattle Manure

0.5 g of the manure obtained from cattle was placed in the anaerobic chamber and diluted with 0.1 ml of Na ₂ S.9H ₂ O and 10 ml of sterile saline and then diluted to 1 volume% with a gas-lift reactor , Park et al., J Ind Microbiol Biotechnol., 40: 995-1003, 2013).

The culture media used were phosphate buffered basal medium (PBBM) containing 0.9 g / L NaCl, 0.32 g / L MgSO ₄ .7H ₂ O, 0.15 g / L CaCl ₂ , 1.0 g / L of NH ₄ Cl, 15.0 mg / L of nitrilotriacetic acid, 1.0 mg / L of FeSO ₄ .7H ₂ O, 1.0 mg / L of MnCl ₂ .4H ₂ O, 1.7 mg / L of CoCl ₂ .6H ₂ O, CaCl of 1.0mg / L of ZnCl _2, 1.0mg / L ₂ and 2H ₂ O, 0.2mg / L of CuCl ₂ and 2H ₂ O, 0.1mg / L of H ₃ BO _3, of 0.1mg / L Na ₂ MoO ₄ , 0.17 mg / L Na ₂ SeO ₃ , 0.26 mg / L NiSO ₄ .6H ₂ O, 10 mg / L NaCl, 0.002 mg / L Biotin, 0.002 mg / L Folic acid, 0.1 mg / L Pyridoxine HCl, 0.05 mg / L Thiamine HCl, 0.05 mg / L Riboflavin, 0.05 mg / L Nicotinic acid, 0.05 mg / L Pantothenic acid, 0.001 mg / L Cyanocobalamine, 0.005 mg / L p- Lipoic acid, 10 mM phosphate buffer, 0.00002% Resazurin, and 2 g / L yeast extract. Eight days after the culture, all of the carbon monoxide was consumed. Dilute the concentrated culture solution to 10 ^{- 7} , and add Hungrate roll tube (Miller et al., Appl Microbiol 27 (5): 985-987, 1974) , And then each of them was inoculated into phosphate buffered basal medium filled with 1 atm of carbon monoxide and cultured with shaking at 180 rpm. The produced organic acids were measured by a gas chromatography-flame ionization detector (GC-FID, ACME 6000, Young Lin Instrument Co., Anyang, Korea) to produce a strain showing the ability to produce isovaleric acid Respectively.

 Example 1.2 Identification of Strain

The selection of the present invention o silica bakteo in (Oscillibacter To identify C5, genomic DNA was isolated from an electric strain using Insta-gene ™ Matrix (Bio rad) product. Using the separated genomic DNA as a template, PCR was performed using primer 1: 5'-AGAGTTTGATCMTGGCTCAG-3 '(SEQ ID NO: 1) and primer 2: 5'-TACGGYTACCTTGTTACG ACTT-3' (SEQ ID NO: 2) chain reaction, PCR) was performed to amplify 16S rDNA. Next, the base sequence of the amplified 16S rDNA was determined and the two strands of the base sequence were ligated using MEGA version 4.0. DNA sequencing was performed with BLAST software (http://www.ncbi.nlm.nih.gov) of the National Center for Biotechnology Information, which is shown in Table 1 below.

As shown in the following table, the strain selected according to the present invention is a strain producing organic acids using carbon monoxide as a substrate (however, Oscillibacter valericigenes and Escherichia coli ), but only 84 to 85% homology. On the other hand, the strain is an Oscillibacter valericigenes ) and 98% homology. A phylogenetic tree based on the above-described homology analysis is shown in FIG.

Class name Code number Homology (%) Oscillibacter < RTI ID = 0.0 > valericigenes ) DSM 18026T AB238598 98 Butyribacterium methylotrophicum ATCC 33266T AF064241.1 85 Acetobacterium carbinolicum DSM 2925T X96956 84 Acetobacterium < RTI ID = 0.0 > fimetarium ) ATCC 51795T X96959 84 Acetobacterium < RTI ID = 0.0 > malicum ) ATCC 51201T X96957 84 Acetobacterium fuldosium ( Acetobacterium paludosum ) ATCC 51793T X96958 85 Acetobacterium < RTI ID = 0.0 > tundra ) DSM 9173T AJ297449) 85 Acetobacterium < RTI ID = 0.0 > woodii ) DSM 1030T NR_026323.1 84 Acetobacterium bakii ) DSM 8239T X96960 84 Clostridium autoethanogenum DSM 10061T < RTI ID = 0.0 > Y18178.1 84 Clostridium carboxidivorans DSM 15243T FR733710.1 84 Clostridium < RTI ID = 0.0 > ragsdalei ) ATCC BAA-622T DQ020022.1 84 Moorella thermoacetica ) ATCC 39289T AJ633105.1 84 Escherichia coli ) AE014075.1 80

As shown in Table 1 and Fig. 3, the isolated strains were Oscillospiraceae family and shows 98% similarity to the closest strain Oscillibacter valericigenes , but the Oshiltera valeriensis is not a carbon monoxide-derived strain (Iino et al., Int J Syst Evol Microbiol., 57 (8): 1840-1845, 2007). As a result, it was found that the strain is a novel strain in oyster barter capable of consuming carbon monoxide. Further, unlike the conventional acetogen, which produces 2 or 4 carbon atoms using carbon monoxide as the anaerobic microorganism, it has the specificity of further producing a compound having 3 or 5 carbon atoms in addition to the carbon atoms of 2 or 4 carbon atoms From the result, the strain was named C5 in oyster.

Example 2. Characterization of C5 in oshilter

The cluster form of C5 in the organism of the present invention was confirmed by the Hunggart roll tube method. The same 80 ml of phosphate buffered basal medium was added to 160 ml of anaerobic microorganism culture glass bottle (Wheaton Scientific Co., Millville, USA), filled with carbon monoxide at 1 atm after sealing, sterilized at 121 ° C for 15 minutes, The genus C5 was inoculated with 1% (v / v) and incubated at 37 ° C with shaking at 180 rpm and grown to OD ₆₀₀ 0.8. The same medium and carbon monoxide were filled in the hungry tube and sterilized with 2.5% (v / v) of agar, and the cultured C5 inoculum was diluted to 10 ^{- 7} and inoculated. After 48 hours, observing the colony formed, the colonies had a whitish smooth surface and were measured at diameters of about 1 to 2 mm. In addition, a single cell of C5 inoculum was observed using a scanning electron microscope (SEM), which is shown in Fig.

As shown in Fig. 4, it can be confirmed that the silicic acid content C5 is in the form of a non-running rod having a size of 0.4 X 1.6 to 0.5 X 2.0 탆.

Test Example 1. Verification of multi-carbon compounds C2 to C5

A gas chromatography-mass spectrometry (GC-MS, Agilent model 6890N, Agilent Technologies, CA, USA) was used to verify the multi-carbon compounds prepared in the present invention. The same 80 ml of phosphate buffered basal medium was added to 160 ml of the anaerobic microorganism-cultivated glass bottle. After sealing, the autoclave was filled with carbon monoxide at 1 atm. After sterilization at 121 ° C for 15 minutes, the pre-cultured microorganism was inoculated with 1% (v / v) And cultured in a shaking incubator at a speed of 180 rpm for 48 hours. After incubation, the culture was pretreated with liquid-liquid extraction and dichloromethane was used as the organic solvent.

As shown in FIG. 5A, acetic acid, propionic acid, isobutyric acid, butyric acid and isovaleric acid were detected through gas chromatography. Qualitative analysis was carried out to verify that isovaleric acid having 5 carbon atoms which was not produced in the conventional acetogen was produced by the Oscillibacter sp. C5 (Accession No: KCTC 12249BP) according to the present invention.

A standard sample for qualitative analysis was 100 ppm (0.915 mM) of isovaleric acid standard solution (Sigma-aldrich 99%, USA, product number 129542).

The organic acids separated by gas chromatography were ionized by mass spectrometer ion source electron energy (70 eV) and separated by mass to charge ratio (m / z) through quadrupole. FIG. 5 is a graph showing a sample obtained by a liquid-liquid extraction with a reference material in a SIM (Selective Ion Monitoring) mode in which only specific ions of a gas chromatography mass spectrometer can be selected. FIG. It is a graph comparing the relative intensity of each ion with the built-in library. As shown in Fig. 5, peaks were confirmed at the same time zone (14.2 minutes) between the reference material and the sample. It was also found that the cleavage tendencies of the ions measured by mass versus charge were almost similar. As can be seen in FIG. 6, the relative intensities of the respective ions were analyzed and the library was searched. As a result, it was found that the degree of similarity of isovaleric acid (3-Methylbutanoic acid) was high.

Korea Research Institute of Bioscience and Biotechnology KCTC12249BP 20120723

Claims (14)

Oscillibacter sp. C5 (Accession No .: KCTC 12249BP) isolated from bovine anger having the characteristics of producing multi-carbon compounds having 2 to 5 carbon atoms. The Oscillobacter sp. C5 according to claim 1, wherein the carbon monoxide gas source is used as a substrate. [Deposit number: KCTC 12249BP]. [Claim 3] The process according to claim 2, wherein the carbon monoxide gas source is a synthesis gas. O5 C5 [Deposit number: KCTC 12249BP] Oscillibacter sp. [Claim 4] The method according to claim 2, wherein the carbon black compound having 2 to 5 carbon atoms is at least one selected from the group consisting of acetic acid, propionic acid, butyric acid, ethanol, propanol, butanol, pentanol and isovaleric acid Oscillibacter sp. C5 [Accession No .: KCTC 12249BP]. The method of claim 1, wherein the O-silico bakteo in C5 (Oscillibacter sp.) [Accession number: KCTC 12249BP] is in O-silico bakteo characterized in that the anaerobic microorganisms C5 (Oscillibacter sp.) [Accession number: KCTC 12249BP]. 2. The method according to claim 1, wherein the Oscillibacter sp. C5 (Accession No: KCTC 12249BP) has a gene encoding an enzyme involved in the Wood-Ljungdahl pathway. Oscillibacter sp. C5 [Accession No .: KCTC 12249BP]. 6. The method of claim 5, wherein the gene coding for the enzymes involved in the wood-feeding pathway is acetyl coA synthase, carbon monoxide dehydrogenase, Formyltetrahydrofolate cyclohexanone, formyltetrahydrofolate synthase, formimidotetrahydrofolate cyclodeaminase, formyltetrahydrofolate cyclohydrolase / methylenetetrahydrofolatedhydrogenase and methylene tetrahydrophosphate, ( OTC ) C5 [accession number: KCTC 12249BP] which is at least one selected from the group consisting of genes coding for methylene tetrahydrofolate reductase, Filling the medium with carbon monoxide; And a step of inoculating and culturing Oscillibacter sp. C5 (accession number: KCTC 12249BP). 9. The method according to claim 8, wherein the culture is carried out under anaerobic conditions. 9. The method of claim 8, wherein the culturing is carried out with shaking culture at 25 to 50 DEG C and 50 to 500 rpm for 12 to 72 hours. 9. The method according to claim 8, wherein the culture is performed at a pH of 5.5 to 8.0. The method of claim 8, further comprising extracting the carbon compound having 2 to 5 carbon atoms using an organic solvent after completion of culturing. The method of claim 8, wherein the culturing is by the carbon monoxide as a substrate O-silico bakteo in C5: characterized in that it comprises the step of carbon atoms is produced from 2 to 5 carbon compound by [accession number KCTC 12249BP] (Oscillibacter sp.) Wherein the carbon number is 2 to 5. 9. The method of claim 8, wherein the carbon compound having 2 to 5 carbon atoms is at least one selected from the group consisting of acetic acid, propionic acid, butyric acid, ethanol, propanol, butanol, pentanol, and isovaleric acid. 2 to 5 carbon atoms.

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