KR20150030150A - Method and apparatus of manufacturing cosmetic products for regenerating skin cell - Google Patents

Abstract

The present invention relates to a method and apparatus for manufacturing a cosmetic product for skin cell generation, capable of manufacturing a cosmetic product for skin cell generation with excellent absorption using functional water similar to bio-water, wherein the cosmetic product for skin cell generation is manufactured by generating magnetized hexagonal water from water, by a magnetized hexagonal water; extracting natural protein from a stem cell culture liquid, which is obtained by safely separating human fat stem cells and then culturing the cells, by a first extractor; obtaining at least one skin useful ingredient of peptide, niacinamide, adenosine, arbutin, palmitoyl tripeptide-5, lecithin, squalane, ubiquinone, hyaluronic acid, allantoin, and collagen from the stem cell culture liquid, by a second extractor; obtaining a plant extract from at least one plant of pomegranate, ginseng, licorice, ginseng seeds, Astragalus membranaceus, Wolfiporia cocos, Scutellaria baicalensis, Portulaca oleracea, Polygonum multiflorum, Angelica gigas, Angelica tenuissima, Coix lachryma-jobi, Coconopsis lanceolata, Morus alba, and cucumber, by a third extractor; and mixing the natural protein, at least one skin useful ingredient, and at least one plant extract using the magnetized hexagonal water, by a mixer.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a skin cell regeneration cosmetic product,

The present invention relates to a method and an apparatus for producing cosmetics for regenerating skin cells, and more particularly, to a method for producing cosmetics for regenerating skin cells, ≪ / RTI >

Stem cells are capable of differentiating into other cells through appropriate environment and stimulation, and are capable of self-proliferation. They are embryonic stem cells (ES cells) isolated from early embryos, embryonic stem cells Three types of embryonic germ cells (EG cells) isolated from primordial germ cells and multi-potent adult progenitor cells (MAP cells) isolated from the adult bone marrow are most well known. Since stem cells have the potential to develop into cells with characteristic development and specialized functions, they have been studied as a cell resource for functional recovery of various organs.

Korean Patent Laid-Open No. 10-2013-0008293 discloses a method for mass-producing growth factors and compositions for skin regeneration using fetal-derived mesenchymal stem cells in amniotic fluid cultured under low oxygen conditions. In the amniotic fluid obtained from a mother Separating fetal-derived cells; Isolated fetal-derived cells are subcultured in a medium containing FBS (fetal bovine serum) and bFGF (basic fibroblast growth factor) to obtain amniotic fluid-derived mesenchymal stem cells; And culturing the obtained mesenchymal stem cells in a low oxygen state. According to this disclosed technique, since amniotic mesenchymal stem cells can be obtained by culturing fetal cells derived from amniotic fluid, another source other than bone marrow-derived mesenchymal stem cells can be made available, and embryo-derived mesenchymal stem The cells can be cultured for 3 days in a low oxygen state containing 1% oxygen and further cultured for 3 days in a serum-free medium to produce a large amount of growth factors in the conditioned medium.

Korean Patent Laid-Open No. 10-2013-0003715 discloses a method for producing a culture solution of an amniotic fluid fetal-derived mesenchymal stem cell using a wound stimulus, comprising the steps of: applying a wound stimulus to a stem cell; And culturing the stem cells in a serum-free medium for 1 to 10 days to prepare a conditioned medium. According to the disclosed technology, it is possible to provide a composition having better cell growth and skin regeneration ability by using a culture solution of stem cells prepared by applying a wound stimulus.

In preparing the composition for skin regeneration as described above, in the prior art, water is mainly used as a rinse water to prepare a composition for skin cell regeneration, whereby the cluster formation of water is low and absorption power into the skin is small Penetration of the skin cell regeneration composition into the skin is not easy, and the skin is easily deteriorated by the external environment, which causes troubles on the skin.

Korean Patent Publication No. 10-2013-0008293 Korean Patent Publication No. 10-2013-0003715

An embodiment of the present invention is to provide a method and an apparatus for manufacturing skin cell regeneration cosmetics for producing a skin cell regenerating cosmetic product having excellent absorbency using functional water similar to living body water.

Among the embodiments, a method of producing a skin cell regeneration cosmetic product comprises the steps of: generating water with magnetized hexagonal water by a magnetized hexagonal water generator; Extracting natural proteins from a stem cell culture obtained by safely isolating human adipose stem cells by a first extractor; Obtaining at least one skin benefit ingredient selected from the group consisting of peptides, niacinamide, adenosine, arbutin, palmitoyl tripeptide-5, lecithin, squalane, ubiquinone hyaluronic acid, allantoin and collagen from the stem cell culture solution by a second extractor; Obtaining a plant extract from at least one plant selected from the group consisting of pomegranate, red ginseng, licorice, ginseng seed, hwanggi, gyeongryeong, golden, marcheon, hansuo, angelica, gobon, niin, doduk, mallow and cucumber by a third extractor; And mixing the natural protein, the at least one skin benefit ingredient, and the at least one plant extract using the magnetized hexagonal water by a mixer and a blender to prepare a cosmetic for skin cell regeneration.

In one embodiment, the step of generating magnetized hexagons may change the water into magnetized hexagons with a magnetic force of 10,000 gauss to 13,500 gauss generated at the N pole by a magnetic body installed to maintain a constant gap.

In one embodiment, the step of generating with the magnetized hexagonal number may result in a cluster having an interfacial tension of 40 to 50 (dyne / cm) to produce a small magnetized hexagonal number.

In one embodiment, the step of generating magnetized hexagons may include vibrating a magnetic body installed to maintain a constant gap by ultrasonic vibration of 30 to 130 (Hz) generated by an ultrasonic generator, sterilizing water, Can be generated.

In one embodiment, the step of extracting the natural protein comprises culturing the differentiated cells while suppressing the growth of the differentiated cells when subculturing the somata derived from the human adipose tissue stem cells to form differentiated cells, Can be recovered.

In one embodiment, the solution component may contain growth factors, procollagen, fibronectin.

In one embodiment, the growth factor may include an epithelial growth factor, a fibroblast growth factor, a skin cell growth factor, a hepatocyte growth factor, an insulin-like growth factor, a transforming growth factor, a vascular endothelial growth factor.

In one embodiment, the method for manufacturing a skin cell regeneration cosmetic product comprises regulating the weight of the magnetized hexagonal water, the natural protein, the at least one skin benefit ingredient, and the at least one plant extract at a predetermined blending ratio, And the step of magnetizing and hexagonally converting the skin cell regenerating cosmetics by the magnetizing and hatching number generator.

Among the embodiments, an apparatus for producing cosmetics for regenerating skin cells comprises: a magnetized hexagonal water generator which is supplied with water and is produced as magnetized hexagonal water; A first extractor for extracting natural proteins from a stem cell culture obtained by safely isolating human adipose stem cells and culturing them; A second extractor for obtaining at least one skin benefit ingredient from the stem cell culture solution, the peptide, niacinamide, adenosine, arbutin, palmitoyl tripeptide-5, lecithin, squalane, ubiquinone hyaluronic acid, allantoin and collagen; A third extractor for obtaining a plant extract from at least one plant selected from the group consisting of pomegranate, red ginseng, licorice, ginseng seed, hwanggi, gyeonggi, golden, marcheon, And a blending machine for blending the natural protein, the at least one skin benefit ingredient, and the at least one plant extract using the magnetized hexahedra to produce a cosmetic for skin cell regeneration.

In one embodiment, the magnetizing hexagonal-number generator comprises: a magnetic body installed to maintain a constant gap for generating a magnetic force of 10,000 gauss to 13,500 gauss generated at the N pole; A water inflow portion for introducing water into the magnetic body; And an ultrasonic generator generating ultrasonic vibration of 30 to 130 (Hz) to vibrate the magnetic body to sterilize the water and generate magnetized hexagonal water.

In one embodiment, the magnetizing horn angle generator is configured to adjust the weight of the magnetized hexagonal number, the natural protein, the at least one skin benefit component and the at least one plant extract at a predetermined compounding ratio, Cosmetics can be magnetized and hexagonalized.

In one embodiment, the apparatus for manufacturing skin cell regeneration cosmetics further comprises: a carrier supplier for supplying a pharmaceutically or cosmetically acceptable carrier as needed; And a composition generator for generating a cosmetic composition or a pharmaceutical composition containing the skin cell regeneration cosmetic prepared in the compounder, as an active ingredient, by supplying the carrier from the carrier supplier.

In one embodiment, the apparatus for manufacturing a skin cell regeneration cosmetic product further comprises a formulation maker for producing a cosmetic composition or a pharmaceutical composition containing the skin cell regeneration cosmetic manufactured by the above-mentioned compounding unit as an effective ingredient in a predetermined formulation .

The method and apparatus for manufacturing a skin cell for regeneration of skin cells according to an embodiment of the present invention can produce skin cell regenerating cosmetics with excellent absorbency by using functional water similar to biological water, It is easy to penetrate the skin of cosmetics for regeneration of skin cell because it absorbs into the skin. It helps restoring the skin to its original condition without any skin troubles. Also, it strengthens skin's natural health and moisturizing power, increases skin collagen synthesis Improve wrinkles, reduce pores, and increase skin elasticity.

1 is a flowchart illustrating a method of manufacturing a skin cell regeneration cosmetic product according to an embodiment of the present invention.
2 is a view for explaining an apparatus for manufacturing a skin cell regeneration cosmetic product according to an embodiment of the present invention.
Fig. 3 is a view for explaining the blender shown in Fig. 2. Fig.

The description of the present invention is merely an example for structural or functional explanation, and the scope of the present invention should not be construed as being limited by the embodiments described in the text. That is, the embodiments are to be construed as being variously embodied and having various forms, so that the scope of the present invention should be understood to include equivalents capable of realizing technical ideas. Also, the purpose or effect of the present invention should not be construed as limiting the scope of the present invention, since it does not mean that a specific embodiment should include all or only such effect.

Meanwhile, the meaning of the terms described in the present invention should be understood as follows.

The terms "first "," second ", and the like are intended to distinguish one element from another, and the scope of the right should not be limited by these terms. For example, the first component may be referred to as a second component, and similarly, the second component may also be referred to as a first component.

It is to be understood that when an element is referred to as being "connected" to another element, it may be directly connected to the other element, but there may be other elements in between. On the other hand, when an element is referred to as being "directly connected" to another element, it should be understood that there are no other elements in between. On the other hand, other expressions that describe the relationship between components, such as "between" and "between" or "neighboring to" and "directly adjacent to" should be interpreted as well.

It should be understood that the singular " include "or" have "are to be construed as including a stated feature, number, step, operation, component, It is to be understood that the combination is intended to specify that it does not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

All terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, unless otherwise defined. Commonly used predefined terms should be interpreted to be consistent with the meanings in the context of the related art and can not be interpreted as having ideal or overly formal meaning unless explicitly defined in the present invention.

1 is a flowchart illustrating a method of manufacturing a skin cell regeneration cosmetic product according to an embodiment of the present invention.

Referring to FIG. 1, the magnetization hexahedron generator generates water as magnetized hexahedra (S101). The magnetized hexagons correspond to function numbers similar to the number of living organisms in the body. Water is magnetized and hexagonalized by being influenced by a strong magnetic field generated by a magnetic body (that is, a permanent magnet) installed into the magnetized hexagonal generator and installed to maintain a constant gap in the magnetized hexagonal generator.

In one embodiment, the magnetizing hexagonal-number generator forms a strong magnetic force (for example, 10,000 to 13,500 Gauss magnetic force) generated from the N pole of a magnetic body that maintains a certain gap therein, Can be magnetized and hexagonalized. The magnetizing hexagonal generator can magnetize and hexagonalize water to remove common bacteria, E. coli, etc. contained in water. The magnetic body can rotate the water in the direction of 90 degrees of the magnetic field to smooth the formation of magnetized hexagons and increase the kinetic energy of the water by rotating the impeller or the like, thereby facilitating the formation of clusters of water. Accordingly, the magnetized hexagonal water generator can generate a larger amount of water at high speed with magnetized hexagonal water, and can reduce the cluster of water to enhance the penetration into the human body.

The magnetizing hexagonal generator has a lower interfacial tension (ie, 40 to 50 (dyne / cm)) than water because the water can not pass through the cells inside and outside of the cell bound to the cell wall with an interfacial tension of 72 (dyne / cm) A magnetized hexagonal cell having a size smaller than that of the magnetized hexagonal cell generated through the use of water can be formed through the cell cluster to pass through the cell wall.

In one embodiment, the magnetizing hexagonal generator may include a magnetic body that maintains a constant gap in the interior, and an ultrasonic generator that generates ultrasonic waves in the gap. The ultrasonic generator may generate ultrasonic vibration to cause the magnetic body to vibrate by ultrasonic vibration. The magnetic body generates a magnetic force generated from the N pole at 10,000 gauss to 13,500 gauss and the ultrasonic generator generates ultrasonic vibration at 30 to 130 (Hz) (preferably 52 (Hz)) to generate a certain gap It is possible to sterilize the water introduced through the water and simultaneously generate water as magnetized hexagonal water.

In one embodiment, the ultrasonic generator includes an oscillator and a vibrator, and the oscillator can oscillate the oscillator so that the oscillator generates a frequency of 20,000 or more per second. The ultrasonic generator can diverge the oscillating magnetic field so that the magnetic body is vibrated by ultrasonic waves generated by the oscillator. When a magnetic field changes due to vibration, the magnetic body changes the magnetic property of water flowing through a certain gap in the magnetizing hexagonal generator to change the magnetic field, thereby assisting the generation of magnetized hexagonal magnetism along with sterilization of water.

The first extractor separates human adipose tissue stem cells and extracts natural proteins from a stem cell culture fluid containing human adipose stem cells (S102).

Human adipose stem cells play a very important role in the production and regeneration of the skin, which is the beginning of all body tissues and also exists in the skin, allowing aging or damaged skin to regenerate into new cells.

Stem cell culture fluid has 130 different nutrients that are secreted during the cultivation of human adipose tissue stem cells. It has skin regeneration ability, it helps the collagen production of the skin as well as prevents pigmentation and makes transparent and elastic skin .

In one embodiment, differentiated cells derived from human adipose stem cells can be subcultured to form differentiated cells, while at the same time, differentiated cells can be cultured while suppressing the growth of differentiated cells, thereby recovering the solution components.

In one embodiment, the first extractor is capable of culturing a colony extracted from human adipose stem cells into an ampoule upon differentiation cell formation. For example, culturing from a colony to an amphibian can be performed for 5 days to 7 days, and subculture of the amphibian can be carried out for 5 to 10 passages to form differentiated cells with vigorous growth.

In one embodiment, the solution component can be obtained from cultured differentiated cells and stem cell culture fluids when the differentiated cells are cultured in a predetermined culture medium (for example, a stem cell culture medium). The solution component may include a liquid component of the stem cell culture fluid and a liquid metabolite produced upon culturing the differentiated cells. The solution components can be recovered after the differentiated cells have been cultured for 1 to 5 days.

In one embodiment, the solution component may include growth factors, procollagen with a promoting resilience function, and fibronectin with a wound healing function as a skin constituent. The growth factor is a natural skin regeneration material in the human body. It is an epitope cell growth factor which is a polypeptide formed from 53 kinds of amino acids, fibroblast growth factor having a function of skin cell growth, skin ulcer and wound healing and keratinization A stem cell growth factor for skin regeneration such as a skin cell growth factor having a re-epithelialization function for promoting proliferation differentiation of cells, and the like. Growth factors include, but are not limited to, inducing cellular activity, hepatocyte growth factors with surgical wound healing function, insulin-like growth factors with tissue regeneration and growth promotion, repair of wounded tissue, transforming growth factors with regulation of immune cell proliferation, A vascular endothelial growth factor which induces cell growth and division and promotes vascular cell migration and has an increasing function of skin constitution.

The second extractor extracts at least one skin benefit component from the stem cell culture solution (S103), at least one of peptides, niacinamide, adenosine, arbutin, palmitoyl tripeptide-5, lecithin, squalane, ubiquinone hyaluronic acid, allantoin and collagen.

Skin benefit ingredients can help to whiten skin, improve wrinkles, calm skin, moisturize skin and increase elasticity. At least one skin benefit component may be selected by the user.

The third extractor extracts the plant extract from at least one of plants such as pomegranate, red ginseng, licorice, ginseng seed, hwanggi, gyeongryeong, golden, marcheon,

Plant extracts help to calm skin, moisturize the skin and increase elasticity, and balance the oil balance and skin cycle to bring the skin to life. At least one or more plant extracts may be selected by the user.

In the blender, a skin cell regenerating cosmetic product is prepared by mixing magnetized hexagonal water, natural protein, at least one skin benefit ingredient, and at least one plant extract (S105).

In one embodiment, the blender can control the compounding ratio of the magnetized hexagons, the natural protein, the at least one skin benefit ingredient, and the at least one plant extract to produce a cosmetic for skin cell regeneration with the best skin absorption power. For example, the blender may be provided by a user with a blending ratio of 50% (%) magnetized Hexagon, 5% (%) natural protein, 2% (%) at least one skin benefit ingredient, Lt; / RTI >

In one embodiment, the cosmetic for regenerating skin cells may have higher skin absorbency through magnetization and hexagonalization steps by the magnetizing hexagonal generator. The skin cell regeneration cosmetic comprises a cosmetic composition, and the cosmetic composition may contain a carrier. The cosmetic composition may further comprise other ingredients such as lubricants, wetting agents, sweetening agents, flavoring agents, emulsifying agents, suspending agents and preservatives. The cosmetic composition can be prepared in various formulations.

For example, the cosmetic composition may be formulated as a suspension, emulsion, paste, gel, cream, lotion, powder, wax or spray. The cosmetic composition may be, for example, an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier in the case of a paste, a cream or a gel. The cosmetic composition may be exemplified by lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder, when the formulation is a powder or a spray. When the formulation is an emulsion, the cosmetic composition may contain at least one selected from the group consisting of ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, butylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, Or fatty acid esters of sorbitan. When the formulation of the cosmetic composition is a suspension, examples of the carrier include a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or trachant.

In one embodiment, the cosmetic composition may contain cosmetics for skin cell regeneration in an amount of from 0.1 (%) to 10 (%) by weight based on the total weight of the cosmetic composition. For example, cosmetics for skin cell regeneration may be contained in an amount of 5 (%) by weight based on the total weight of the cosmetic composition.

2 is a view for explaining an apparatus for manufacturing a skin cell regeneration cosmetic product according to an embodiment of the present invention.

Referring to FIG. 2, an apparatus 100 for manufacturing skin cell regeneration cosmetics comprises a first magnetic hexagonal square generator 110, a first extractor 120, a second extractor 130, a third extractor 140, (150).

The first magnetic hexahedron generator 110 includes a water inlet and a magnetic body. The first magnetic hexahedron generator 110 includes a water inlet portion and a magnetic body. The water inlet portion generates water (for example, groundwater, rock water, deep water water, etc.) (Magnetic force of 10,000 Gauss to 13,500 Gauss) generated at the N pole, which is beneficial to the human body, is formed inside the first magnetization hexahedral generator 110 with a certain gap therebetween. Water supplied from the water inlet is generated as magnetized hexagonal water, and the generated magnetized hexagonal water is supplied to the blender 150.

In one embodiment, the first magnetization hexahedron generator 110 may further include an ultrasonic generator for generating ultrasonic waves in a gap between the magnetic bodies. The ultrasonic wave generating part generates ultrasonic waves and vibrates the magnetic bodies by the vibrations caused by the ultrasonic waves so that the water introduced into the gaps of the magnetic bodies can be generated as small magnetized hexagons in the clusters.

In one embodiment, the ultrasonic generator can oscillate the oscillator to oscillate at a frequency of 20,000 or more per second oscillating in the crystal oscillator, oscillate the magnetic body by the oscillation of the oscillator to diverge the oscillating magnetic field, By changing the magnetic property of the water introduced into the gap of the magnetic body by the oscillating magnetic field, it is possible to make the magnetized hexagonal crystal with sterilization.

The first extractor 120 extracts the natural protein from the stem cell culture liquid obtained by safely separating human adipose stem cells and then supplies the extracted natural protein to the blender 150.

In one embodiment, the first extractor 120 is adapted to cultivate differentiated cells while suppressing the growth of differentiated cells when subculturing the somata derived from human adipose tissue stem cells to form differentiated cells, The solution component containing the lectin can be recovered.

The second extractor 130 extracts human adipose stem cells from the stem cell culture solution obtained by safely isolating human adipose stem cells and then cultivating the cells in a culture medium containing peptide, niacinamide, adenosine, arbutin, palmitoyl tripeptide-5, lecithin, squalane, ubiquinone hyaluronic acid, , Collagen, and the like, extracts one or more skin benefit components from the extracted skin benefit component groups, and supplies the selected skin benefit components to the blender 150.

The third extractor 140 extracts plant extracts from plant groups such as pomegranate, red ginseng, licorice, ginseng seed, hwanggi, gyeongryeong, golden, marcheon, At least one of the obtained plant extracts is selected, and the selected plant extracts are supplied to the compounder 150.

The blender 150 mixes the natural protein supplied from the first extractor 120, the at least one skin benefit component supplied from the second extractor 130, and the skin benefit component supplied from the second extractor 130, using the magnetized hexagonal water supplied from the first magnetization hexahedron generator 110, And the third extractor 140 are mixed together to prepare a skin cell regenerating cosmetic.

In one embodiment, the blender 150 includes a magnetizing hexagonal column supplied from the first magnetizing hexagonal generator 110, a natural protein supplied from the first extractor 120, at least one skin benefit from the second extractor 130 And the weight of one or more kinds of plant extracts supplied from the third extractor 140 can be adjusted according to a predetermined blending ratio to produce a skin cell regeneration cosmetic having the best skin absorption power.

In one embodiment, the apparatus 100 for manufacturing skin cell regeneration cosmetics may further include a second magnetization hexahedron generator 160. At this time, the blender 150 can supply the manufactured skin cell regenerating cosmetics to the second magnetic hexahedron generator 160, and the second magnetic hexahedron generator 160 generates cosmetics for skin cell regeneration supplied from the blender 150 Can be magnetized and hexagonalized to produce cosmetics for regenerating skin cells having higher skin absorption power.

In one embodiment, the apparatus 100 for manufacturing skin cell regeneration cosmetics comprises a composition generator for producing a cosmetic composition or a pharmaceutical composition containing the skin cell regeneration cosmetic prepared in the compounder 150 as an active ingredient, May further comprise a carrier feeder for feeding the composition generator with a pharmaceutically or cosmetically acceptable carrier according to the invention.

In one embodiment, an apparatus 100 for manufacturing skin cell regeneration cosmetics comprises a skin cell regeneration apparatus 100 for producing a cosmetic composition or a pharmaceutical composition containing the skin cell regeneration cosmetic prepared in the compounder 150 as an active ingredient in a predetermined formulation And may further comprise a formulation maker.

Fig. 3 is a view for explaining the blender shown in Fig. 2. Fig.

Referring to FIG. 3, the blender 150 includes a first blender 151, a carrier feeder 152, and a second blender 153.

The first compounder 151 extracts natural proteins supplied from the first extractor 120 and one or more skin benefit components supplied from the second extractor 130 using the magnetized hexagonal water supplied from the magnetizing horn axis generator 110, And the third extractor 140 are mixed to prepare a skin cell regeneration cosmetic, and the manufactured skin cell regeneration cosmetics are supplied to the second blender 153.

The carrier feeder 152 supplies the second blender 153 with a pharmaceutically or cosmetically acceptable carrier as needed.

In one embodiment, the carrier feeder 152 may supply to the second blender 153 a carrier further containing other components such as lubricants, wetting agents, sweeteners, flavoring agents, emulsifying agents, suspending agents, preservatives and the like.

The second blender 153 mixes the skin cell regeneration cosmetic supplied from the first blender 151 and the carrier supplied from the carrier feeder 152 to produce a cosmetic composition or a pharmaceutical composition, 151) as an effective ingredient to produce a cosmetic composition or a pharmaceutical composition.

In one embodiment, the second blender 153 can be combined with the skin cell regenerating cosmetics produced in the blender 150 to produce a cosmetic or pharmaceutical composition, wherein the carrier supplied from the carrier feeder 152 is combined.

In one embodiment, the blender 150 is provided with a cosmetic composition or a pharmaceutical composition produced in the second blender 153, and a formulation maker 154 ).

In one embodiment, the formulation maker 154 may be formulated with a cosmetic composition in the form of a suspension, emulsion, paste, gel, cream, lotion, powder, wax or spray.

In one embodiment, the formulation maker 154 is configured to produce a pharmaceutical composition comprising an animal feed, a vegetable oil, a wax, a paraffin, a starch, a tracant, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc or zinc oxide Or the like can be used to formulate the cosmetic composition as a paste, cream or gel.

In one embodiment, the formulation maker 154 uses a carrier, such as lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder, supplied from a carrier feeder 152 to formulate the cosmetic composition into a powder or spray Can be generated. In addition, the formulation maker can be used in combination with fatty acid esters of ethanol, isopropanol, ethylcarbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, butylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan, The same carrier can be used to formulate the cosmetic composition as an emulsion.

In one embodiment, the formulation maker 154 includes a liquid diluent, such as ethanol or propylene glycol, fed from a carrier feeder 152, such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester A carrier such as a suspending agent, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant may be used to formulate a formulation of the cosmetic composition as a suspension.

Hereinafter, the present invention will be described in more detail with reference to examples, but it goes without saying that the scope of the present invention is not limited to these examples.

Example 1. Wound healing effect of human adipose tissue stem cells

 [Table 1]

As shown in Table 1, collagen expression in human adipose tissue stem cell culture medium (ADSC-CM) was higher than collagen expression in human fibroblast culture medium (HDF-CM) ((A) and (B) (ADSC-CM), the higher the concentration of human adipose stem cell culture medium (ADSC-CM), the higher the collagen expression ((C) and (D)).

[Table 2]

Referring to Table 2 above, experiments on human adipose stem cell culture (ADSC-CM) based on biological effects in relation to migration and wound healing of human fibroblast (HDF) are shown.

The mRNA was expressed in collagen 1, collagen 3, fibroblast, and MMP1 detected by RT-PCR (A) and compared with β-actin (B). The expression of collagen 1, collagen 3, and fibroblasts increases with the concentration of human adipose stem cell culture medium (ADSC-CM). That is, the higher the concentration of the human adipose stem cell culture medium (ADSC-CM), the better the wound healing.

[Table 3]

Referring to Table 3 above, it shows wound healing effects of human adipose tissue stem cells (ADSC) through a mouse experiment. It can be seen that healing through human adipose tissue stem cells (ADSC) reduced the wound area compared to no treatment when 24 hours after surgery.

Related literature 1: Wound healing effect of adipose-derived stem cells: A critical role of secretory factors on human dermal fibroblasts

Document 2: Hypoxia-enhanced wound-healing function of

Adipose-derived stem cells: Increase in stem cell proliferation and up-regulation of VEGF and bFGF

Related literature 3: The wound-healing and antioxidant effects of adipose-derived stem cells

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention as defined by the following claims It can be understood that

100: Apparatus for manufacturing skin cell regeneration cosmetics
110: First magnetization hexagonal generator
120: first extractor
130: Second extractor
140: Third extractor
150: Mixer
160: Second magnetization hexagonal generator
151: a first blender
152: carrier feeder
153: the second blender
154: Formulation machine

Claims (13)

Generating magnetized hexagonal water through the magnetized hexagonal generator;
Separating human adipose stem cells from the skin of a human body through a first extractor and extracting natural proteins from a stem cell culture fluid containing human adipose stem cells;
Extracting at least one skin benefit component of the peptide, niacinamide, adenosine, arbutin, palmitoyl tripeptide-5, lecithin, squalane, ubiquinone hyaluronic acid, allantoin and collagen from the stem cell culture solution through a second extractor;
Extracting a plant extract from at least one plant selected from the group consisting of pomegranate, red ginseng, licorice, ginseng seed, hwanggi, gyeongryeong, golden, marcheon, ginseng, ginseng, gobon, ginseng, And
Preparing a skin cell regeneration cosmetic product by blending the magnetized hexagonal water, the natural protein, the at least one skin benefit component, and the at least one plant extract through a blender.
The method of claim 1, wherein generating the water with magnetized hexagons comprises:
And converting the water into magnetized hexagonal water with a magnetic force of 10,000 G (gauss) to 13,500 G (gauss) generated at the N pole of the magnetic body maintaining a constant gap.
The method of claim 1, wherein generating the water with magnetized hexagons comprises:
Generating a magnetized hexagonal crystal having a size smaller than that of the magnetized hexagonal crystal by using the cluster having an interfacial tension of 40 to 50 (dyne / cm) lower than that of the water, A method for producing cosmetics for cell regeneration.
The method of claim 1, wherein generating the water with magnetized hexagons comprises:
Comprising the steps of sterilizing the water and generating the water as magnetized hexagonal water when a magnetic material having a constant gap is vibrated by the ultrasonic vibration of 30 to 130 (Hz) generated in the ultrasonic generator. A method for manufacturing a cosmetic product for regeneration.
The method of claim 1, wherein extracting the natural protein comprises:
Characterized in that the differentiated cells derived from the human adipose stem cells are subcultured to form differentiated cells and at the same time the differentiated cells are cultured while the growth of the differentiated cells is suppressed to recover the solution components Gt;
6. The method according to claim 5,
Growth factor, procollagen, and fibronectin. ≪ RTI ID = 0.0 > 11. < / RTI >
7. The method of claim 6,
A skin cell growth factor, a hepatocyte growth factor, an insulin-like growth factor, a transforming growth factor, and a vascular endothelial growth factor, which is characterized in that it comprises at least one of an epithelial growth factor, a fibroblast growth factor, a skin cell growth factor, Gt;
The method according to claim 1,
Wherein the at least one skin benefit component and the weight of the at least one plant extract are adjusted to a predetermined mixing ratio, and then the skin cell regenerating cosmetic is immersed in the magnetized and hexagonal water through the magnetized hexagonal water generator, Wherein the method further comprises the step of forming the skin cell.
A magnetized hexagonal water generator for generating water as a magnetized hexagonal water by receiving water;
A first extractor for separating human adipose stem cells from human skin and extracting natural proteins from a stem cell culture fluid containing human adipose stem cells;
A second extractor for extracting at least one skin benefit component selected from the group consisting of peptides, niacinamide, adenosine, arbutin, palmitoyl tripeptide-5, lecithin, squalane, ubiquinone hyaluronic acid, allantoin and collagen from the stem cell culture solution;
A third extractor for obtaining a plant extract from at least one plant selected from the group consisting of pomegranate, red ginseng, licorice, ginseng seed, hwanggi, gyeongryeong, golden, marcheon, And
And a blender which mixes the magnetized hexagonal water, the natural protein, the at least one skin benefit component, and the at least one plant extract to produce a skin cell regenerating cosmetic product.
10. The apparatus of claim 9, wherein the magnetizing hexagonal-
A magnetic body provided to maintain a constant gap and forming a magnetic force of 10,000 G (gauss) to 13,500 G (gauss) generated at the N pole;
A water inflow portion for introducing water into the magnetic body; And
And an ultrasonic generator for vibrating the magnetic body by ultrasonic vibration of 30 to 130 (Hz) to sterilize the water and generate the water as magnetized hexagonal water.
10. The apparatus of claim 9, wherein the magnetizing hexagonal-
Characterized in that the skin cell regeneration cosmetics are magnetized and hexavalent after adjusting the weight of the magnetized hexagons, the natural protein, the at least one skin benefit component and the weight of the at least one plant extract to a predetermined blending ratio Apparatus for manufacturing cosmetics for skin cell regeneration.
10. The method of claim 9,
A carrier supplier for supplying a cosmetically acceptable carrier; And
Further comprising a composition generator for supplying a carrier from the carrier supplier and producing a cosmetic composition containing skin cell regeneration cosmetics manufactured by the blender as an active ingredient.
13. The method of claim 12,
Further comprising a formulation maker for producing a cosmetic composition containing the skin cell regeneration cosmetic prepared in said compounder as an effective ingredient in a predetermined formulation.

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