KR20150017119A - Water soluble milk thistle extract and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a method for preparing a water-soluble milk thistle extract, the method comprising the steps of: dissolving a milk thistle extract in alcohol and dissolving cyclodextrin in water; mixing the milk thistle extract-dissolved solution and the cyclodextrin-dissolved solution; lowering the concentration of alcohol in the mixture by stages; and encapsulating the milk thistle extract in cyclodextrin. According to the present invention, the preparation of the water-soluble milk thistle extract by the preparation method of the present invention can simplify the preparation process and provide a water-soluble milk thistle extract which has a reduced bitter taste, is stable against heat and pH, and has a high content of silymarin due to an increased recovery rate of silymarin.

Description

[0001] The present invention relates to a water soluble milk seed extract and a preparation method thereof,

The present invention relates to a water-soluble milk seed extract and a preparation method thereof, and more particularly to a method for producing a water-soluble milk seed extract by dissolving a milk seed extract in ethanol and dissolving cyclodextrin in water; Mixing the milk seed extract solution with a cyclodextrin dissolution solution; Stepwise lowering the concentration of ethanol in the mixture; And encapsulating the milk seed extract with cyclodextrin. The present invention also relates to a method for producing a water-soluble milk seed extract.

Milk seeds ( silybum marianum or carduus marianus ) is known for its efficacy in the treatment and prevention of various liver and gallbladder dysfunctions. Silymarin is mainly composed of silybin A and B, isosilybin A and B, silicristin and silidianin as main components of milk seed extract. Because silymarin is a lipid soluble ingredient, it is not soluble in water and therefore can not be used in beverage formulations. The method of forming a capsule with a water-soluble medium, cyclodextrin, for the water-soluble medium is carried out in a water-soluble state. , All of the milk seed extract is flocculated as it contacts with water and aggregates into lumps. The recovery rate of silymarin after incoupling is as low as 20% or less because it forms a cluster before forming an inclusion compound in an aqueous solution state. In the present invention, in order to facilitate the production of the water-soluble milk seed extract, the milk seed extract is dissolved in ethanol, the beta-cyclodextrin is dissolved in water to confirm that each substance is completely dissolved in the solvent, The concentration of the silymarin is lowered step by step to increase the recovery rate of the silymarin from 80% to 90%.

Cyclodextrin is a non-reducing malto-oligosaccharide in which 6-8 glucose molecules are in the form of cyclic α-1,4 bonds and consists of a donut structure of a converted form, The hydroxyl groups (-OH) bonded to the carbon atoms 2 and 3 are hydrogen-bonded to form a hydrophobic structure. On the contrary, the hydroxyl groups are hydrophilic to the outside by a highly polar hydroxyl group. Due to this property, the cyclodextrin is hydrophobic And an inclusion compound to increase the solubility of the hydrophobic substance and increase the stability of the unstable substance. In addition, it can be used to remove high molecular or odor by enclosing a part or the whole of organic molecules exhibiting taste and flavor in a cavity (Pro. Biochem., 39, pp. 1033-1046, 2003; Chem. Rev., 98 , pp. 1743-1753, 1998; J. Inclusion Phenomena and Macrocyclic Chem., 36, pp. 355-370, 2000).

A generally used cyclodextrin solution is a complex of cyclodextrin alpha, beta and gamma forms, produced by the action of an enzyme called cyclodextrin trans glycosidase in a medium containing starch, LD50 values are reported to be as high as 10 g / kg or more, and only a trace amount is absorbed in the gastrointestinal tract. Among them, beta-cyclodextrin is the most commonly used substance in pharmaceuticals and foodstuffs, and its safety has been acknowledged. As a result, it has been found that the LD50 values of oral administration to rats and mice are 12, It is a safe material with 12.5g / kg or more.

 Previous studies for the aqueous solution of milk seeds extracts have been reported to dissolve silibinin in bases in " silybinin entrapped material, its preparation method and pharmaceutical composition containing it " (Korean Patent Laid-Open No. 10-1992-0007625) A method of encapsulation has been studied, which involves the use of ammonium hydroxide to convert to a base and the addition of acid and salt removal to neutralize again. It is a process of dissolving silymarin in a base state and encapsulating it. However, there is a concern about loss of components due to a change in pH, and a process cost for removing salts generated during neutralization occurs.

Accordingly, the present inventors have made extensive efforts to overcome the problems of the prior art. As a result, in the case of producing a water-soluble milk seed extract by the production method provided in the present invention, the manufacturing process is simplified and the bitterness And is stable to heat and pH, and the recovery rate of silymarin is increased, so that a water-soluble milk seed extract containing a high content can be produced. Thus, the present invention has been completed.

Accordingly, it is a main object of the present invention to provide a method for producing a water-soluble milk seed extract containing a high content by simplifying the manufacturing process, alleviating the bitter taste of milk seeds, stabilizing it in heat and pH, and increasing the recovery rate of silymarin .

It is another object of the present invention to provide a water-soluble milk seed extract having a silymarin content of 5% or more by using the above-described method for producing an aqueous milk cedar extract.

It is still another object of the present invention to provide a health functional food containing the water-soluble milk seed extract prepared using the method for producing the water-soluble milk seed extract.

According to one aspect of the present invention, the present invention provides a method for producing a protein, comprising dissolving a milk seed extract in ethanol and dissolving the cyclodextrin in water; Mixing the milk seed extract solution with a cyclodextrin dissolution solution; Stepwise lowering the concentration of ethanol in the mixture; And encapsulating the milk seed extract with cyclodextrin. The present invention also provides a method for producing a water-soluble milk seed extract.

According to the present invention, there is provided a process for producing a water-soluble milk seed extract, which comprises the following steps.

1) a first step of dissolving a milk seed extract in ethanol and dissolving cyclodextrin in water;

2) a second step of mixing the milk seed extract solution and the cyclodextrin dissolving solution at 4.5-5.5: 4.5-5.5 (v: v), preferably 5: 5 (v: v);

3) adding a cyclodextrin solution to the mixture to adjust the mixing ratio to 2.5 to 3.5: 6.5 to 7.5 (v: v), preferably 3: 7 (v: v);

4) adding a cyclodextrin solution to the mixture to adjust the mixing ratio to 0.5 to 1.5: 8.5 to 9.5 (v: v), preferably 1: 9 (v: v);

5) Concentrating the mixture to remove ethanol to convert it into an aqueous solution;

6) encapsulating the milk seed extract with cyclodextrin in an aqueous solution;

7) filtering to remove the unencapsulated residue; And

8) Step 8 to dry the cloth.

According to the present invention, it is possible to add 0.1 to 20% (w / w), more preferably 3 to 5% (w / w) of the surfactant to the weight of the solid content of the mixed solution in order to increase inclusion activity. Surfactants include lecithin, sugar esters, phospholipids, sorbitan fatty acid esters, polyglycerin esters, and tween series. The addition of the surfactant is preferably carried out as it is in the liquid state, and it is preferable to add the surfactant in the form of a water-soluble one.

In the present invention, the milk seed extract has a silymarin content of 30 to 70% (w / w).

The silymarin is a lipid-soluble component as a main component of the milk seed extract and can not be used in water-soluble beverage formulations because it is not soluble in water.

In the present invention, the cyclodextrin is a beta-cyclodextrin, gamma-cyclodextrin, or alpha-cyclodextrin alone or a mixture thereof.

Since beta-cyclodextrin is superior in increasing the solubility by forming a stable inclusion compound with fat-soluble ingredients and gamma-cyclodextrin is effective in eliminating the bitter taste, it is more efficient to mix these two ingredients than when using a single ingredient to be.

In the present invention, in the first step, the dissolution concentration of the milk seed extract may be 1 to 30% (w / v) and the beta-cyclodextrin dissolution concentration may be 1 to 40% (w / v) (W / v) and the beta-cyclodextrin dissolution concentration is 1 to 15% (w / v).

In the present invention, in the second, third and fourth steps, when the milk seed extract solution and the beta-cyclodextrin solution are mixed, the temperature condition is 50 to 90 ° C, the stirring time is 1 to 5 hours, Is set to 100 rpm or more.

In the present invention, when the milk seed extract solution and the beta-cyclodextrin dissolution solution are mixed in the second, third and fourth steps, the ethanol purity of the step is 45 to 50% in the second step, 30%, and 5% to 10% in the fourth step.

According to the present invention, the concentration of ethanol is lowered stepwise to facilitate the ethanol removal, while simplifying the process of producing the water-soluble milk seed extract.

In the present invention, in the fifth step, the mixture of the solution of the extract of milk seed extract and the cyclodextrin is concentrated at 30 to 50 ° C to completely remove ethanol.

According to the present invention, in the sixth step, when the milk seed extract is encapsulated in cyclodextrin, the temperature is 50 to 90 ° C, the stirring time is 1 to 5 hours, and the stirring speed is 100 rpm or more.

In the seventh step, the diatomaceous earth may be added in an amount of 1 to 5% (w / v), preferably 2 to 3% (w / v) And the filtrate is maintained at a temperature of 50 to 60 ° C.

When the diatomaceous earth content is less than 1%, it is not easy to remove the non-water soluble milk seed extract and the filtrate may become turbid. In this case, the solubility of the water-soluble milk seed extract may not be dissolvable, and if it is 5% or more, the filtration property improves. However, the yield depends on the amount of diatomaceous earth, thereby affecting the yield of production and the recovery rate of silymarin. But it does not make a big difference at 5%.

In the present invention, in the eighth step, the method for drying the covering material may be carried out by any method conventionally used, but preferably by spray drying, lyophilization, or hot air drying.

In the present invention, the water-soluble milk seed extract is characterized in that the recovery rate of silymarin is 80% or more.

According to the embodiment of the present invention, the water-soluble milk seed extract prepared by the method of the present invention has a recovery rate of silymarin of 80% or more and a recovery rate of 4 times or more higher than that of conventional silymarin recovery (less than 20%). From these results, it can be seen that the water-soluble milk seed extract prepared by the method of the present invention is highly useful for preparing a water-soluble milk seed extract because of high recovery of silymarin after inclusion.

According to another aspect of the present invention, there is provided a water soluble milk seed extract having a silymarin content of 5% or more, which is produced by the method of any one of claims 1 to 3.

According to the embodiment of the present invention, the content of silymarin in the milk-seed extract prepared by the above method was 5% or more. In the case of milk seed extract, the content of silymarin was as high as 60%. However, the yield of silymarin in the preparation of water-soluble milk seed extract was less than 20% and the content of silymarin present in the final foam was less than 1.3% Very low. On the other hand, the water-soluble milk seed extract of the present invention showed higher silymarin content and recovery than the water-soluble milk seed extract prepared by the conventional method. From these results, it can be seen that the process of the present invention is capable of producing a water-soluble milk seed extract having a high content of silymarin and a high recovery rate.

In the present invention, the milk seed extract is characterized in that the redness is reduced.

According to the example of the present invention, the water-soluble milk seed extract of the present invention was found to have an improved bitter taste as compared with the control group. In the case of Example 3, the redness was the least, which is attributable to the use of gamma-cyclodextrin having an effect of eliminating the bitter taste. From these results, it can be seen that the water-soluble milk seed extract prepared by the production method of the present invention can be used effectively in the production of a health functional food by reducing the red ginseng (Experimental Example 4).

According to another aspect of the present invention, the present invention provides a health functional food containing a water-soluble milk seed extract.

According to the embodiment of the present invention, the water-soluble milk seed extract prepared by the production method of the present invention has excellent thermal stability (FIG. 2) and pH stability (FIG. 3) ). As a result, when the water-soluble milk seed extract prepared by the production method of the present invention is contained as an active ingredient of the health functional food, the bitter taste of the milk seed extract is reduced, the stability of the silymarin is improved, It is possible to provide a health functional food improved in quality by increasing transparency without forming a precipitate at the time of use.

In the present invention, the extract is contained in any one selected from the group consisting of capsules, tablets, powders, granules, liquids and rings.

According to the present invention, when the extract of the present invention is used as a food additive, it can be used as it is or can be used together with other food or food ingredients, and can be suitably used according to a conventional method. The ingredients other than the extract may be appropriately selected and blended by those skilled in the art according to the formulations, and the active ingredients may be used alone or in combination of two or more. The amount of mixing can be suitably determined according to the intended use (prevention, health or therapeutic treatment).

When the water-soluble milk seed extract is prepared by the production method of the present invention, the process can be simplified, and a water-soluble milk seed extract containing a high content of silymarin and having a high recovery rate can be produced. In addition, the water-soluble milk seed extract prepared through the above method has a reduced redness, is stable to heat and pH, and has excellent solubility. Therefore, it can be used as a raw material for health functional foods and cosmetic compositions, and particularly useful for liquid formulation .

1 is a schematic view of a process for producing a water-soluble milk seed extract.
FIG. 2 shows the stability of the silymarin when heat-treating the water-soluble milk seed extract.
FIG. 3 shows the stability of the silymarin when the water-soluble milk seed extract was treated by pH.
FIG. 4 shows the stability of the water soluble milk seed extract after 30 days of dissolution at 1%, 2% and 3%.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example  1: water soluble Milk seeds  Preparation of extract

Milk seeds were degreased with hexane, extracted with ethanol, filtered, concentrated, and dried. Then, 1,000 mL of ethanol was added to 100 g of the milk seed extract and dissolved by stirring at 70 rpm for 30 minutes at 100 rpm. 1,000 g of beta-cyclodextrin (CAVAMAX 占 W7, Wacker, Germany) was added with 10 L of constant water and dissolved by stirring at 70 占 폚 for 30 minutes at 100 rpm. 1 L of a solution of beta-cyclodextrin dissolved in 1 L of a milk seed solution was slowly added and stirred at 70 rpm for 2 hours at 100 rpm. Beta-cyclodextrin solution was further added, followed by stirring at 70 DEG C for 2 hours at 100 rpm. Thereafter, 6.7 L of a solution of beta-cyclodextrin was further added, and the mixture was stirred at 70 rpm for 2 hours at 100 rpm. The mixture was concentrated (PAL-3, ATAGO) to a sugar content of 20% at 40 to 50 ° C to remove ethanol. After stirring at 70 DEG C for 1 hour, 300 g of diatomaceous earth (IMERYS, France) was added, followed by compression filtration. Sufficiently washed with a constant 2 L of 70 DEG C, and spray dried to obtain 935 g.

Example  2: water soluble Milk seeds  Preparation of extract

Milk seeds were degreased with hexane, extracted with ethanol, filtered, concentrated, and dried. Then, 1,000 mL of ethanol was added to 100 g of the milk seed extract and dissolved by stirring at 70 rpm for 30 minutes at 100 rpm. 10 g of constant water was added to 500 g of beta-cyclodextrin (CAVAMAX 占 W7, Wacker, Germany) and dissolved by stirring at 70 占 폚 for 30 minutes at 100 rpm. 1 L of a solution of beta-cyclodextrin dissolved in 1 L of a milk seed solution was slowly added and stirred at 70 rpm for 2 hours at 100 rpm. Beta-cyclodextrin solution was further added, followed by stirring at 70 DEG C for 2 hours at 100 rpm. 6.7 L of a solution of beta-cyclodextrin dissolved therein was further added, followed by stirring at 70 rpm for 2 hours at 100 rpm. The mixture was concentrated (PAL-3, ATAGO, Japan) at a sugar content of 20% at 40 to 50 ° C to remove ethanol. After stirring at 70 DEG C for 1 hour, 300 g of diatomaceous earth (IMERYS, France) was added, followed by compression filtration. Washed thoroughly with 2 L of constant temperature at 70 캜, and spray dried to obtain 512 g.

Example  3. Water solubility Milk seeds  Preparation of extract

Milk seeds were degreased with hexane, extracted with ethanol, filtered, concentrated, and dried. Then, 1,000 mL of ethanol was added to 100 g of the milk seed extract and dissolved by stirring at 70 rpm for 30 minutes at 100 rpm. 10 g of constant water was added to 400 g of beta-cyclodextrin (CAVAMAX 占 W7, Wacker, Germany) and 100 g of gamma-cyclodextrin (CAVAMAX 占 W8, Wacker, Germany) and dissolved by stirring at 70 占 폚 for 30 minutes at 100 rpm. 1 L of the mixed solution of cyclodextrin was gradually added to 1 L of the milk seed solution, followed by stirring at 70 rpm for 2 hours at 100 rpm. 1.3 L of cyclodextrin mixed solution was further added, and the mixture was stirred at 70 rpm for 2 hours at 100 rpm. 6.7 L of cyclodextrin mixed solution was added, and the mixture was stirred at 70 rpm for 2 hours at 100 rpm. The mixture was concentrated (PAL-3, ATAGO, Japan) at a sugar content of 20% at 40 to 50 ° C to remove ethanol. After stirring at 70 DEG C for 1 hour, 300 g of diatomaceous earth (IMERYS, France) was added, followed by compression filtration. Sufficiently washed with a constant 2 L of 70 DEG C, and spray dried to obtain 478 g.

Example  4. Water solubility Milk seeds  Preparation of extract

Milk seeds were degreased with hexane, extracted with ethanol, filtered, concentrated, and dried. Then, 1,000 mL of ethanol was added to 100 g of the milk seed extract and dissolved by stirring at 70 rpm for 30 minutes at 100 rpm. 10 g of constant water was added to 400 g of beta-cyclodextrin (CAVAMAX 占 W7, Wacker, Germany) and 100 g of alpha-cyclodextrin (CAVAMAX 占 W7, Wacker, Germany) and dissolved by stirring at 70 占 폚 for 30 minutes at 100 rpm. 1 L of the mixed solution of cyclodextrin was gradually added to 1 L of the milk seed solution, followed by stirring at 70 rpm for 2 hours at 100 rpm. 1.3 L of cyclodextrin mixed solution was further added, and the mixture was stirred at 70 rpm for 2 hours at 100 rpm. 6.7 L of cyclodextrin mixed solution was added, and the mixture was stirred at 70 rpm for 2 hours at 100 rpm. The mixture was concentrated (PAL-3, ATAGO, Japan) at a sugar content of 20% at 40 to 50 ° C to remove ethanol. After stirring at 70 DEG C for 1 hour, 300 g of diatomaceous earth (IMERYS, France) was added, followed by compression filtration. Sufficiently washed with a constant 2 L of 70 DEG C, and spray dried to obtain 467 g.

Experimental Example  One : Silymarin  Content analysis

(Alliance, Waters, USA) to determine the content of silymarin, the active ingredient of the water-soluble milk seedless extract prepared in Examples 1 to 4. The column was Capcell Pak C18 25cm * 4.5mm, 5um, Shiseido, Japan), and the detection spectrum was measured using UV 288nm. Mobile phase A was mixed with methanol, distilled water and phosphoric acid at 20: 80: 0.5 (v: v: v) and mobile phase B was mixed with methanol, distilled water and phosphoric acid at 80: 20: 0.5 (v: v: v). The conditions of the mobile phase over time were analyzed by changing the mobile phase B to 15%, the mobile phase B to 45%, the mobile phase B to 15%, the mobile phase B to 15%, and the flow rate to 0.3 ml / min. Silychristin, silydianin, silybin, isosilybin A and isosilybin B (Sigma, St-Louis, Mo, USA) were dissolved in methanol at a concentration of 1 mg / ml and used as a stock solution. And the content of silymarin was measured. The results are shown in Table 1.

Experimental group Production (g) Content of silymarin (%) Recovery rate of silymarin (%) Milk seed extract 61.4 Example 1 935 5.84 88.9 Example 2 512 10.47 87.3 Example 3 478 10.35 80.6 Example 4 467 10.55 80.2

As a result, as shown in Table 1, the content of silymarin in the water-soluble milk seed extract was 5 to 10% (w / w) and 80 to 90 (%) showed a high recovery rate.

Experimental Example  2: water soluble Milk seeds  Thermal Stability of Extracts

6 g of the water-soluble milk seed extract prepared in Example 1 was dissolved in 200 ml of constant water. The thermal stability was confirmed by analysis of silymarin by heat treatment in Autoclave 90 for 1, 2, 4, 6 hours. Control group was converted to relative content after 100% silymarin before heat treatment. As a result, it was confirmed that about 5% was decomposed to about 95% after 6 hours (FIG. 2).

Experimental Example  3: water soluble Milk seeds  Extract pH  stability

6 g of the water-soluble milk seed extract prepared in Example 1 was dissolved in 200 ml of constant water. The pH was adjusted to 5.3, 4.1, 3.1 with 0.1 N HCl. The pH of the soluble milk cow extract solution before pH adjustment is 6.15. Before the pH treatment, 100% of silymarin was used as a control group and the relative content was calculated. As a result, when the silymarin content was measured at all pH on the 7th day, the content was maintained at about 100% (FIG. 3).

Experimental Example  4: water soluble Milk seeds  Sensory Evaluation of Extracts

Milk seed extract and water soluble milk seed extract were dissolved in water at a concentration of 1 w / v%, respectively, and then the difference in bitter taste was measured. The sensory evaluation was performed on 15 sensory workers who were well trained in aroma, taste, color and texture. The control group (10%) was used as a control group (milk cedar extract) , Very bad: 9 points), and the results were expressed as mean or mean ± standard deviation. The test was repeated three or more times and Duncan's multiple range test was used to verify its significance. The results are shown in Table 2.

Test group bitter Control group 10 Example 1 3.2 ± 0.48 Example 2 4.3 ± 0.39 Example 3 1.5 ± 0.24 Example 4 4.8 ± 0.57

As a result, as shown in Table 2, it was confirmed that the bitter taste was improved in proportion to the content of the water-soluble milk seed extract in comparison with the control group.

Experimental Example  5: water soluble Milk seeds  Solubility of the extract

Example 1 was dissolved in 1%, 2% and 3% of dissolution water, and sterilized at 95 ° C for 5 minutes, and the change with time was measured at room temperature for 30 days. As a result, it was confirmed that no precipitate was formed on the naked eye even after 30 days passed (FIG. 4).

Claims (16)

Dissolving the milk seed extract in ethanol and dissolving the cyclodextrin in water; Mixing the milk seed extract solution with a cyclodextrin dissolution solution; Stepwise lowering the concentration of ethanol in the mixture; And encapsulating the milk seed extract with cyclodextrin. ≪ RTI ID = 0.0 > 21. < / RTI >
The method of claim 1, comprising the steps of:
1) a first step of dissolving a milk seed extract in ethanol and dissolving cyclodextrin in water;
2) a second step of mixing the milk seed extract solution and the cyclodextrin dissolving solution at 4.5 to 5.5: 4.5 to 5.5 (v: v);
3) adding a cyclodextrin solution to the mixture to adjust the mixing ratio to 2.5 to 3.5: 6.5 to 7.5 (v: v);
4) adding a cyclodextrin solution to the mixture to adjust the mixing ratio to 0.5 to 1.5: 8.5 to 9.5 (v: v);
5) Concentrating the mixture to remove ethanol to convert it to an aqueous phase;
6) encapsulating the milk seed extract with cyclodextrin in an aqueous solution;
7) filtering to remove the unencapsulated residue; And
8) Step 8 to dry the cloth.
3. The method according to claim 2, wherein in the first step, the milk seed extract has a content of silymarin of 30 to 70% (w / w).
3. The method of claim 2, wherein the cyclodextrin is beta-cyclodextrin, gamma-cyclodextrin, or alpha-cyclodextrin alone or a mixture thereof.
The method according to claim 2, wherein, in the first step, the dissolved concentration of the milk seed extract is 1 to 30% (w / v) and the beta-cyclodextrin dissolution concentration is 1 to 40% (w / v) How to.
The method according to claim 2, wherein, in the second, third, and fourth steps, the temperature of the solution of the milk seed extract and the solution of the beta-cyclodextrin is 50 to 90 ° C, the stirring time is 1 to 5 hours, Wherein the speed is 100 rpm or more.
The method according to claim 2, wherein, in the second, third, and fourth steps, the ethanol purity is 45-50% in step 2 when mixed with the solution of milk seed extract and beta-cyclodextrin dissolution, To 30%, and in the fourth step, from 5% to 10%.
[Claim 3] The method according to claim 2, wherein in the fifth step, the mixture solution of the milk seed extract solution and the cyclodextrin is concentrated at 30 to 50 DEG C to completely remove ethanol.
The method according to claim 2, wherein, in the sixth step, the milk seed extract is encapsulated in cyclodextrin at a temperature of 50 to 90 ° C, a stirring time of 1 to 5 hours, and a stirring speed of 100 rpm or more Way.
The method according to claim 2, wherein, in the seventh step, diatomaceous earth is added in an amount of 1 to 5% (w / v) relative to the liquid amount after inclusion to remove unencapsulated residues and the temperature is maintained at 50 to 60 ° C Lt; / RTI >
The method according to claim 2, wherein in the eighth step, the foam is dried by spray drying, lyophilization, or hot air drying.
3. The method according to claim 2, wherein the recovery rate of silymarin is 80% or more.
A water-soluble milk seed extract having a silymarin content of 5% or more prepared by the method of claim 1 or 2.
14. The water-soluble milk seed extract according to claim 13, wherein the redness is reduced.
A health functional food containing the water-soluble milk seed extract according to claim 13.
16. The health functional food according to claim 15, wherein the extract is contained in any one selected from a capsule, a tablet, a powder, a granule, a liquid, and a ring.

Images