KR20140137867A - Composition comprising herbal mixture extract for treating or preventing inflammatory disease - Google Patents

Abstract

The present invention relates to a composition comprising a mixture extract of Acer tegmentosum M. and Eucommia ulmoides Oliveras as an active ingredient for preventing or treating an inflammatory disease. The mixture extract of Acer tegmentosum M. and Eucommia ulmoides Oliveras according to the present invention has remarkably excellent anti-inflammatory effects than a single extract such as reduction of inflammatory reaction derived by LPS and the likes; thereby can be valuably used for preventing or treating the inflammatory disease.

Description

[0001] The present invention relates to a composition for preventing or treating an inflammatory disease,

The present invention relates to a composition for the prophylaxis or treatment of inflammatory diseases, which comprises as an active ingredient, a mixed extract of a mountain elk and two mites.

Immunity is a kind of protection mechanism for the human body to protect itself during the microbial invasion process, in which tissues, cells and molecules protect the organ against the infectious agent. Immune surveillance systems are classified into innate immunosurveillance and adaptive immunosurveillance. The innate immune system consists of leukocytes and cytokines, including macrophages and natural killer cells, and plays a primary defense role by reacting quickly before adaptive immunity to infection occurs. In the innate immune system, macrophages, which are responsible for the frontiers of biological defense mechanisms, play a very important role in the immune system as part of host defense mechanisms. They are the first to recognize foreign substance intrusion and are involved in humoral immunity and cellular immunity. When activated, not only the morphological changes of the cells such as the proliferation and spreading ability, but also the enhancement of the macrophage capacity, the improvement of the NO (nitric oxide) and the cytokine production, and finally the growth of cancer cells and various harmful bacteria ≪ / RTI > In recent years, there has been a growing interest in research aimed at promoting such immune actions as natural substances.

Inflammation reactions are bio-defense reactions caused by various factors such as infection by pathogens or tissue damage, and they perform an initial protective action to confine the damage only to the infected area or damaged area. In most cases, this inflammatory response leads to the elimination of pathogenic factors and the induction of specific adaptive immunity using components of endogenous immunity.

 On the other hand, Acer tegmentosum M. is a deciduous broad-leaved arboreous tree of Aceraceae, which is distributed only in mountainous areas in the north of central part of Korea, in Manchuria, It is also known as a tree or a suture. It was found that the mountain elk had an inhibitory effect on cancer cell growth in gastric cancer, liver cancer, lung cancer and breast cancer cells.

 Eucommia ulmoides Oliver is a deciduous arboreous tree belonging to Eucommiaceae, which is known as the origin of Hubei and Sichuan provinces in China. It is known to have tonic, sedative and analgesic effects and has been used to prevent diseases such as hypertension due to blood pressure lowering action.

As described above, although various pharmacological effects of the acid elk and the two species are known, there is little research on the anti-inflammatory activity of the mixed extract thereof.

As a result of continuing efforts to develop new anti-inflammatory agents derived from natural materials, the inventors of the present invention have found that the mixed extracts of acid eletris and two mites have an excellent effect of reducing the inflammatory response induced by LPS , Thereby completing the present invention.

It is an object of the present invention to provide a pharmaceutical composition for the prevention or treatment of inflammatory diseases, which comprises a mixed extract of a mountain elk and two mites as an active ingredient.

It is still another object of the present invention to provide a food composition for preventing or ameliorating an inflammatory disease, which comprises a mixed extract of a mountain elk and two mites as an active ingredient.

In order to achieve the above object, the present invention provides a pharmaceutical composition for the prevention or treatment of an inflammatory disease, which comprises a mixed extract of a mountain elk and two insects as an active ingredient.

The present invention also provides a food composition for preventing or ameliorating an inflammatory disease, which comprises a mixed extract of a mountain elk and two mites as an active ingredient.

The mixed extract of a plant or a plant of the present invention has remarkably excellent antiinflammatory effect compared to a single extract, such as reducing the inflammatory response induced by LPS, and thus can be effectively used for the prevention or treatment of inflammatory diseases .

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a graph showing the antioxidative activity of a mixed extract of a mountain elk (chrysanthemum morifolium) and two mites of the present invention. Fig.
Fig. 2 is a graph showing the anti-inflammatory activity of a mixed extract of a mountain elk (aquamarina) of the present invention and a mite.

The present invention provides a composition for the prevention or treatment of inflammatory diseases, which comprises a mixed extract of a mountain elk and two mites as an active ingredient.

The composition comprises a pharmaceutical composition or a food composition.

Hereinafter, the present invention will be described in more detail.

Mixed extracts of the active ingredients, acid elk and two mites, in the composition of the present invention can be obtained by the following method.

First, the mountain elm and the two insects are washed with water to remove foreign matter, and then dried and crushed in the shade. Cultivated or harvested species can be used without limitation. A solvent having a volume of about 2 to 20 times, preferably 3 to 10 times the volume of the above-mentioned sunflower or quadricepsum powder is added to complete immersion. The extraction solvent is not limited thereto, but at least one solvent selected from water, alcohols having 1 to 4 carbon atoms, and a mixed solvent thereof may be used, and water is preferable. The extraction method can be impregnated or warmed at room temperature, and is preferably extracted at 50 to 90 占 폚, more preferably 85 to 90 占 폚. The extract is filtered and concentrated under reduced pressure to obtain an acid elek extract or mulberry extract, which are then mixed to obtain a mixed extract of a mountain elk and two mites.

The mixing ratio of the above-mentioned mountain elk and the two species is in a weight ratio of 60 to 70: 30 to 40, preferably 65: 35. When the mixing ratio is out of the above range, there is no significant difference in the anti-inflammatory effect as compared with the case of using alone or in combination with the extract of mulberry or mulberry, and there is a risk of side effects.

The mixed extracts of the present invention of the present invention have remarkably excellent anti-inflammatory effect as compared with the single extracts such as reducing the inflammatory response induced by LPS, and thus can be usefully used for the prevention or treatment of inflammatory diseases .

The inflammatory disease includes all common inflammatory diseases and is preferably selected from the group consisting of gastritis, rhinitis, conjunctivitis, asthma, dermatitis, atopic dermatitis, inflammatory bowel disease, inflammatory liver disease, inflammatory lung disease, inflammatory vascular disease, inflammatory rheumatoid arthritis, Psoriasis, multiple sclerosis and venous sinusitis, and the like.

The composition of the present invention may contain at least one known active ingredient having antiinflammatory effect together with a mixed extract of a mountain elk and two mites.

The compositions of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the manufacture of pharmaceutical compositions. In addition, it can be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, oral preparations such as syrups and aerosols, external preparations, suppositories and sterilized injection solutions according to a conventional method. Suitable formulations known in the art are preferably those as disclosed in Remington ' s Pharmaceutical Science, recently, Mack Publishing Company, Easton PA. Examples of carriers, excipients and diluents that can be included in the composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose , Microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When the composition is formulated, it is prepared using a diluent such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, or an excipient usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose, lactose, Gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the non-aqueous solvent and suspension include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The term "administering" as used herein is meant to provide any desired composition of the invention to a subject in any suitable manner.

The preferred dosage of the pharmaceutical composition of the present invention varies depending on the condition and body weight of the individual, the degree of disease, the drug form, the route of administration and the period of time, and can be appropriately selected by those skilled in the art. For the desired effect, the mixed extract of the present invention may be administered in an amount of 1 mg / kg to 10000 mg / kg per day, and may be administered once or several times a day.

The pharmaceutical composition of the present invention may be administered to a subject in various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine dural or intracerebral injection.

The pharmaceutical composition of the present invention can be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers for the prevention or treatment of inflammatory diseases.

In the present invention, the health functional food refers to a food having a biological control function such as prevention or improvement of disease, bio-defense, immunity, recovery after disease, suppression of aging, and should be harmless to human body when taken over a long period of time. The mixed extract of the present invention of the present invention can be added to a health functional food for the purpose of preventing or improving an inflammatory disease. When the mixed extract of the present invention is used as a food additive, the mixed extract of the above-mentioned acid elk and the two mites can be directly added or used together with other food or food ingredients, and can be appropriately used according to a conventional method . The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). Generally, in the production of foods or beverages, the mixed extracts of the present invention and the double crops of the present invention are added in an amount of not more than 15% by weight, preferably not more than 10% by weight based on the raw material. However, in the case of long-term intake for the purpose of health and hygiene or for the purpose of controlling health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

There is no particular limitation on the kind of the food. Examples of foods to which the above substances can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen and other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. The natural carbohydrates may be monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, natural sweeteners such as dextrin and cyclodextrin, synthetic sweeteners such as saccharine and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 10 g, preferably about 0.01 to 0.1 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention may further contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, A carbonating agent used in a carbonated beverage, and the like. In addition, the composition of the present invention may comprise flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. Although the ratio of such additives is not critical, it is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, preferred embodiments, experimental examples, and production examples are provided to facilitate understanding of the present invention. However, the following examples, experimental examples and production examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the examples, experimental examples and production examples.

EXAMPLES Example 1. Preparation of Mixed Extracts of San.arcoma

1-1. Production of acid elite extract

The mountain elm was washed with water to remove foreign matter, dried in the shade, and pulverized. 1 kg of the above-harvested artichoke powder was extracted with distilled water at 85 ° C to 90 ° C for 24 hours, filtered through filter paper, concentrated, and lyophilized to obtain powdered artichokes.

1-2. Preparation of mulberry extract

After removing the foreign substances by washing with water, they were dried and crushed in the shade. 1 kg of the bamboo powder was extracted with distilled water at 85 ° C to 90 ° C for 24 hours, filtered through filter paper, concentrated and lyophilized to obtain a powdery mugwort extract.

1-3. Mixture of acid elite extract and mulberry extract

The plant extracts and mulberry extracts prepared in Examples 1-1 and 1-2 were homogeneously mixed at a weight ratio (w / w) of 6.5: 3.5 to prepare a powdery mixture.

Experimental Example 1. Evaluation of Antioxidative Activity

In order to evaluate the antioxidative activity of the mixed extracts of the acid eletris and two mites prepared in Example 1, a method of Sakata et al. (Biosci.Biotech. Biochem. 58: 1780-1783, 1994) The radical scavenging ability was evaluated. Ascorbic acid, a powerful antioxidant, was used as a control. The results are shown in Fig.

As shown in FIG. 1, the IC ₅₀ of the mixed extracts of the present invention was found to be less than 10 ug / ml, and it was found to have an excellent antioxidative activity compared to the extract alone and the extracts Respectively.

Experimental Example 2. Evaluation of anti-inflammatory activity

The following experiment was carried out to evaluate the anti-inflammatory activity of the mixed extracts of the acid eletris and two mites prepared in Example 1 above. In the experiment, RAW264.7 cells were used as macrophages, and the cells were used in Korean Cell Line Bank (KTCC, Seoul, Korea). RAW264.7 cells were placed in DMEM medium (Gibco, Grand Island, NY, USA) containing FBS and maintained in an incubator maintained at 37 ° C, 5% CO ₂ . First, Raw264.7 cells were seeded in 96-well plates at a concentration of 2 x ¹⁰ cells / well and cultured for 36 hours. After that, the medium was changed with DMEM medium except for serum, and the cells were further cultured for 24 hours. Each of the wells was treated with the mixed extracts of the acid eletris and two mites prepared in Example 1 to a final concentration of 100 ug / ml. After 2 hours, each well was treated with LPS (lipopolysaccharide) at a final concentration of 1 ug / ml and cultured for 24 hours. After that, MTT solution (5 mg / ml) was added and cultured at 37 ° C for 4 hours. Then, MTT was reduced and the medium was carefully removed so that the formazan produced did not fall on the medium. Finally, 100 μl of DMSO was added to each well, and the absorbance was measured at 540 nm. The results are shown in Fig.

As shown in FIG. 2, in the control group treated with LPS only, about 20% of cell death was induced compared with control, whereas in the group treated with the mixed extract of the present invention, cell survival rate And it was confirmed that the extracts showed better effect than the single extract or the extracts of the extracts of the artichoke alone. From the above results, it was confirmed that the mixed extract of the present invention of the present invention has an excellent effect of inhibiting the inflammatory reaction induced by LPS.

Hereinafter, the pharmaceutical composition of the present invention and the preparation example of the food composition will be described, but the present invention is not intended to be limited but is specifically described .

Formulation Example 1. Preparation of a pharmaceutical composition

1-1. Manufacture of Powder

Mixed extract of mountain elk and two mites 20 mg

Lactose 100 mg

Talc 10 mg

The above components are mixed and filled in airtight bags to prepare powders.

1-2. Manufacture of tablets

Mixed extract of mountain elk and two mites 10 mg

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

1-3. Preparation of capsules

Mixed extract of mountain elk and two mites 10 mg

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

1-4. Injection preparation

Mixed extract of mountain elk and two mites 10 mg

180 mg mannitol

Sterile sterilized water for injection 2974 mg

Na ₂ HPO ₄ .2H ₂ O 26 mg

(2 ml) per 1 ampoule in accordance with the usual injection preparation method.

1-5. Manufacture of liquid agent

Mixed extract of mountain elk and two mites 20 mg

10 g per isomer

5 g mannitol

Purified water . Suitable amount

Each component was added and dissolved in purified water according to the usual liquid preparation method, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and purified water was added thereto. The whole was added with purified water to adjust the total volume to 100 ml, And sterilized to prepare a liquid preparation.

Formulation Example 2. Preparation of Food Composition

2-1. Manufacture of health food

100 mg of mixed extract of mountain elk and two mites

Vitamin mixture quantity

Vitamin A acetate 70 μg

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

0.15 mg of vitamin B2

Vitamin B6 0.5 mg

Vitamin B12 0.2 μg

Vitamin C 10 mg

Biotin 10 μg

Nicotinic acid amide 1.7 mg

Folic acid 50 μg

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

2-2. Manufacture of health drinks

100 mg of mixed extract of mountain elk and two mites

Vitamin C 15 g

Vitamin E (powder) 100 g

19.75 g of ferrous lactate

3.5 g of zinc oxide

Nicotinic acid amide 3.5 g

Vitamin A 0.2 g

Vitamin B1 0.25 g

Vitamin B2 0.3 g

Water quantification

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 DEG C for about 1 hour. The solution thus prepared was filtered and sterilized in a sterilized 2 liter container, It is used in the production of the health beverage composition of the invention.

Although the compositional ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the compounding ratio according to the regional or national preference such as the demand class, the demanding country, and the use purpose.

Claims (6)

The present invention relates to a pharmaceutical composition for preventing or treating an inflammatory disease,
[Claim 2] The method according to claim 1, wherein the mixed extract is obtained by extracting a mixture of an acid eletris and a bug with at least one solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms and a mixed solvent thereof. A pharmaceutical composition for preventing or treating.
The pharmaceutical composition for preventing or treating an inflammatory disease according to claim 1, wherein the mixed extract of a plant or a plant is mixed at a weight ratio of 60: 70 to 30: 40.
The method of claim 1, wherein the inflammatory disease is selected from the group consisting of gastritis, rhinitis, conjunctivitis, asthma, dermatitis, atopic dermatitis, inflammatory bowel disease, inflammatory liver disease, inflammatory lung disease, inflammatory vascular disease, inflammatory rheumatoid arthritis, psoriasis, Wherein the pharmaceutical composition is at least one selected from the group consisting of oral hypogonadism, psoriasis, and sinusitis.
A food composition for preventing or improving an inflammatory disease, comprising as an active ingredient a mixed extract of a plant or a plant.
[Claim 5] The food composition according to claim 5, wherein the mixed extracts of the acid elk and the two insects are mixed at a weight ratio of 60 to 70: 30 to 40: 2.

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