KR20140117003A - A composition comprising the phenolic compounds isolated from extract of Carthamus tinctorious seeds as an active ingredient for skin whitening - Google Patents

Abstract

The present invention relates to a composition comprising a compound isolated from a Carthamus tinctorious seed extract as an active ingredient and, more specifically, to a composition that can be used for a pharmaceutical composition for external skin application or a cosmetic composition for skin whitening by confirming that the compound isolated from the Carthamus tinctorious seed extract has excellent tyrosinase inhibitory activity, melanoma cell viability increase, and melanin biosynthesis inhibitory activity.

Description

[0001] The present invention relates to a whitening composition comprising a compound isolated from a safflower extract as an active ingredient. [0002] The present invention relates to a whitening composition comprising an active ingredient for skin whitening,

The present invention provides a whitening composition comprising, as an active ingredient, a compound isolated from a safflower extract.

[Document 1] Yu YG, Jeong MS, Choe JY, Kim JY. A study on Whitening Effect of Ephedra sinica extracts. Journal of the Korean Society of Design Culture. 31 (2): 153-159 (2005)

[Reference 2] Jeong MH, Kim SS, Kim JS, Lee HJ, Chio GP, Lee HY. Jour. Korean For. Soc . 99 (4): 470-478 (2005)

[Document 3] Bell AA, Weeler MH. Biosynthesis and function of fungal melanin. Annual Review Phytopathology. 24: 411-451 (1986)

[Literature 4] Lerner AB, Fitzpatrick TB. Biochemistry of melanin formation. Physiological Review. 30: 91-126 (1950)

[Literature 5] Chen JS, Wei C, Marxhall MR. Inhibition mechanism of koji acid on polyphenol oxidase. Journal of Agricutural and Food Chemistry. 58: 79-110 (1991)

 [Literature 6] Urabe K, Aroca P, Tsukamoto K, Mascagna D, Paulumbo A, Prota G and Hering VJ. The inherent cytotoxic effect of melanin precursors. 1221: 272-278 (1994)

[Literature 7] Hearing VJ, Tsukamoto K. Enzymatic control of pigmentation in mammals. FASEB J. 5 (14): 2902-2909 (1991)

[Literature 8] Jimenez-Cervantes C, Solano F, Kobayashi T, Urabe K, hearing V, Lezano J, Garcia-Gorreni JC. A new enzymatic function in the melanogenic pathway. J. Biol. Chem. 269 (27): 17993-18000 (1994)

[9] Maeda K, Fukuda M. Arbutin: mechanism of its depigmenting action in human melanocyte culture. J. Phamacol. Exp. Ther. 276: 765-769 (1996)

[Literature 10] Battaini GE, Monzani L, Casella L, Santagostini R, Pagliarin. Inhibition of the catecholase activity of biomimetic dinuclear copper complexes by kojic acid. J. Biol. Inorg. Chem. 5: 262-268 (2000)

[Document 11] Chun HJ, Chli WH, Baek SH, Woo WH. Effect of quercetin on melanogenesis in melan-a melanocyte cells. Korean J. Pharmacogn. 33: 245-251 (2002).

[Document 12] Curto EV, Kwong C, Hermersdorfer H, Glatt C, Santis V, Virador VJ and Dooley TP. Inhibitions of mammalian melanocytes tyrosinase: In vitro comparisons of alkyl esters of gentiic acid with other putative inhibitors. Biochem. Pharmacol. 57: 663-672 (1999)

[Literature 13] Seo BI, Lee ES, Park JH, Kim SC, Byun BH, Choi HY. A efficacy of Korean and Chinese Carthami Semen on analgesic effect, anti-inflammation and arthritis. Kor. J. Herbology. 16 (1): 1-10 (2001)

[14] Hur TY, Yun MY, Cho EH, Lee OJ, Kim KS, Cho NG. Clinical Study on Effect of Carthmi-Flos Herbal Acupuncture Therapy on Osteoarthritis in Knee Joint. The Journol of Korean Acupuncture & Moxibustion Society. 19 (2): 189-200 (2002)

[Document 15] Jang SJ, Lee CH, Yook TH. Effects of Laennec.N.HO ( Hominis placenta. Cervi pontotrionum cornu . Carthami semen ) aqua-acupuncture on the ovariectomized osteoporotic Rats. Korea Institute of Science and Technology Information. 19 (1): 5-18 (1998)

[Document 16] Seung KR, Jung KH. Effect of Carthamus Tinctorius L. Semen on Endotoxin-induced Thrombosis in Rats. The journal of applied pharmacology. 9: 258-262 (2001)

[Document 17] Jung KW, Jeong CS, Jeong JS. Effect of Subfractions of Carthamus tinctorius L. Semen on the Lipid Peroxidation and Oxygen Free Radical Scavenging Enzyme Activities in CCI4-induced Hepatotoxic Rats. J. Fd Hyg. Safety 14 (2): 179-185 (1999)

[18] Sakamura, A., Terayama, Y., Kawakata, S., Ichihara, A. and Saito, H. Conjugated serotonins and phenolic constituents in safflower seed ( Carthamus tinctorius L.). A g ric . B iol . Chem , ≪ / RTI > 44, 2951 (1980).

[19] Michikawa M, Lim KT, McLarnon JG, Kim SU: Oxygen radical-induced neurotoxicity in spinal cord neuron cultures. J. Neurosci Res. 37: 62-70 (1994)

[Literature 20] Carmichael J, Degraff WG, Gadzar AF. 1987. Evaluation of a tetrazolium based semiautomated colorimetric assay: Assessment of chemosensitivity testing. Cancer Res 477: 936-942.

[21] Yagi AT, Kanbara T, Morinobu N. The effect of tyrosinase inhibition for aloe. Planta Med. 3981: 517-519 (1986).

The ozone layer is destroyed due to environmental pollution caused by rapid industrialization, and ultraviolet radiation dose is increasing ( Yu YG, Jeong MS, Choe JY, Kim JY. A study on Whitening Effect of Ephedra sinica extracts. Journal of the Korean Society of Design Culture. 31 (2): 153-159 (2005) ) . Human skin color depends on internal and external factors. Among them, melanin, a natural skin pigment that determines the skin color, is a melanin ( Jeong MH, Kim SS, Kim JS, Lee HJ, Chio GP, Lee HY. Soc ., 99 (4): 470-478 (2005) ).

Melanin improves the survival ability of ultraviolet rays, drying, extreme temperatures, and improves the quality of coffee, tea, and tobacco ( Bell AA, Weeler MH, Biosynthesis and function of fungal melanin, Annual Review Phytopathology 24: 30: 91-126 (1950) ) , excessive melanin synthesis forms spots, freckles, skin spots in the body and promotes skin aging ( J), Wei C, Marxhall MR, Inhibition mechanism of koji acid on polyphenol oxidase, Journal of Agricutural and Food Chemistry 58: 79-110 (1991) Urabe K, Aroca P, Tsukamoto K , Mascagna D, Paulumbo A, Prota G and Hering V. The inherent cytotoxic effects of melanin precursors 1221: 272-278 (1994) ) . The most important step in the biosynthesis of melanin from tyrosine is the initial reaction that occurs through the catalysis of tyrosinase ( Hearing VJ, Tsukamoto K. Enzymatic control of pigmentation in mammals. FASEB J. 5 (14): 2902-2909 (1991)), were attached to an hydroxyl (hydroxyl) of tyrosine (tyrosine) is a mechanism for generating a DOPA (3,4-dihydroxy phenylalanine) ( Jimenez-Cervantes C, Solano F, Kobayashi T, Urabe K, hearing V, Lezano J, Garcia-Gorreni JC. A new enzymatic function in the melanogenic pathway. J. Biol. Chem. 269 (27): 17993-18000 (1994) ) .

Therefore, it is possible to inhibit melanin biosynthesis through inhibition of this reaction. Currently, the most commonly used whitening agent, arbutin, is an inhibitor that competes with L-tyrosine ( Maeda K, Fukuda M. Arbutin: mechanism of its depigmenting action in human melanocyte culture, J. Phamacol. .. Ther 276: 765-769 (1996 )), Abu tin (arbutin), ascorbic acid (ascorbic acid) is a tyrosinase (tyrosinase) the copper ion (copper) of the active site chelating (chelating) to tyrosine (tyrosine) and in inhibiting the process proceeds to DOPA quinone (quinone) as in DOPA and DOPA (Battaini GE, Monzani L, L Casella, Santagostini R, Pagliarin. inhibition of the activity of biomimetic catecholase dinuclear copper complexes by kojic acid. J. Biol Inorg. Chem. 5: 262-268 (2000) ) . However, in the current medical and cosmetic industries, only limited amounts of skin safety and formulation stability have been used ( Chun HJ, Chli WH, Baek SH, Woo WH. 33: 245-251 (2002) Curto EV, Kwong C, Hermersdorfer H, Glatt C, Santis V, Virador VJ and Dooley TP. Inhibitions of mammalian melanocytes tyrosinase: In vitro Comparisons of alkyl esters of gentiic acid with other putative inhibitors. Biochem. Pharmacol. 57: 663-672 (1999) ). In particular, studies using natural herbal medicinal plants have been attracting interest.

Melanin, a key determinant of skin tone, is biosynthesized in melanosomes in pigmented cells called melanocytes in the epidermal basal layer. The starting material for the synthesis of melanin is tyrosine, an amino acid. Tyrosinase plays an important role in the formation of melanin in the skin, tyrosine hydroxylase which oxidizes tyrosine in the melanosome to form DOPA, which acts as a DOPA oxidase which oxidizes DOPA to form DOPA quinone and is an important enzyme for synthesizing melanin polymer .

Carthamus tinctorius L.) is a seed of safflower. Seed of the safflower is harvested in the mature summer season and dried in the sun to be used as a medicament. It is warm and tasty in its nature. ( Seo BI, Lee JS, Kim SC, Byun BH, Choi HY. A efficacy of Korean and Chinese Carthami Semen on analgesic effect, anti-inflammation and arthritis. Kor. J. Herbology. Hur TY, Yun MY, Cho EH, Lee OJ, Kim KS, Cho NG. Clinical Study on Effect of Carthmi-Flos Herbal Acupuncture Therapy on Osteoarthritis in Knee Joint. The Journol of Korean Acupuncture & Moxibustion Society. 19 (2): 189-200 (2002) ) . In addition, it has been reported that safflower is effective for fracture in the private sector, and it has been reported that it has a special effect on the treatment of dog or chicken fracture. Unlike safflower flower, safflower seed is said to have a hemorrhagic action in addition to the action of a hemicycle drug. History of Herbal about is only about yihyeol will act as yakneung of safflower, Oriental classical literature honghwaja is that effective fracture disease does not exist (Jang SJ, Lee CH, Yook TH. effects of Laennec.N.HO (hominis placenta. Cervi pontotrionum cornu . Carthami semen ) aqua-acupuncture on the ovariectomized osteoporotic Rats. Korea Institute of Science and Technology Information. 19 (1): 5-18 (1998) ) .

Recently, it has been reported that coronary artery dilatation and lowering of blood pressure have been shown to be effective in improving hypercholesterolemia and treating thrombosis ( Seung KR, Jung KH. Effect of Carthamus Tinctorius L. Semen on Endotoxin-induced Thrombosis in Rats. The journal of applied pharmacology. 9: 258-262 (2001) ) and is used as a preventive agent for fractures and osteoporosis ( Jung KW, Jeong CS, Jeong JS. Effect of Subfractions of Carthamus tinctorius L. Semen on the Lipid Peroxidation and Oxygen Free Radical Scavenging Enzyme Activities in CCI4-induced Hepatotoxic Rats. J. Fd Hyg. Safety 14 (2): 179-185 (1999) ) .

Therefore, in this study, the compounds isolated from Safflower extract were used as an effective ingredient to inhibit melanin synthesis and to find a natural whitening agent, and to inhibit tyrosinase inhibitory activity, melanin formation inhibitory effect, melanogenesis- Mechanism.

However, it has not been taught or described in the above-mentioned literatures that a compound extract isolated from a safflower extract can be used as a composition for whitening.

Accordingly, the inventors of the present invention found that the compound isolated from the safflower extract is excellent in tyrosinase enzyme inhibition activity, melanoma cell viability increase, melanin biosynthesis inhibition activity, and the like, The present invention has been completed.

In order to attain the above object, the present invention provides a whitening dermatological pharmaceutical composition comprising a compound isolated from a safflower extract as an active ingredient.

The extract of the present invention is characterized by containing 0.1 to 50% by weight based on the total weight of the dermatological pharmaceutical composition.

The pharmaceutical composition includes a cream, a gel, a patch, a spray, an ointment, an alarm, a lotion, a liniment, a pasta or a cataplasmal formulation.

In order to achieve the above object, the present invention provides a whitening cosmetic composition comprising a compound isolated from a safflower extract as an active ingredient.

In addition, the cosmetic composition includes formulations of lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, and pack.

As used herein, a " compound isolated from a safflower extract " refers to a compound selected from acacetin, cosmosiin, N -feruloylserotonin, or N - ( p -coumaroyl) serotonin .

An extract as defined herein is one or more solvents selected from the group consisting of water, ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, butylene glycol, propylene glycol, hydrolyzed butylene glycol, Preferably water and ethanol, most preferably 60% to 90% ethanol soluble extract.

Hereinafter, the present invention will be described in detail.

Herbal medicine extracts and separation compounds of the present invention can be obtained as follows.

For example, the safflower seed of the present invention is pulverized, extracted with a nonpolar solvent such as hexane, ethyl acetate, chloroform and the like, and repeatedly performed to remove fat. The first step is to remove fat, A volume of water of about 1 to 30 times volume, preferably 5 to 15 times, a polar solvent of C 1 to C 4 lower alcohol such as ethanol and methanol, or a mixed solvent thereof, preferably 50% to 90% ethanol For about 3 hours to 12 hours, preferably for about 4 hours to 8 hours at about 50 to 100 캜, preferably about room temperature to 60 캜, is subjected to filtration under reduced pressure, concentration and lyophilization to obtain a safflower seed extract A second step of obtaining; A third step of adding the crude extract obtained in the above step to water by adding about 0.05 to 50 times, preferably 0.5 to 5 times the volume (v / w%) of the weight of the crude extract to the suspension; A non-polar solvent selected from n-hexane, heptane, methylene chloride, chloroform or ethyl acetate in an amount of about 0.5 to 20 times, preferably 1 to 5 times (v / w) A fourth step of sequentially obtaining a non-polar solvent-soluble fraction and a polar solvent-soluble fraction by sequentially repeating fractionation by adding hexane or ethyl acetate solvent, butanol and water; The non-polar solvent-soluble fraction is purified by a column chromatography method such as a silica gel column, a Sephadex column, or an ion exchange resin, or a purification method such as a re-curing method, preferably a developing solvent (developing a mixed solvent of chloroform and water with increasing polarity) (Paclitaxel), N - (feruloylserotonin), N - ( p - aminopyrimidine), and N - ( p - tocopherol) of the present invention through a fourth step of repeating the purification process using a silica gel column and a recrystallization method, Coumaroyl) serotonin), and the like.

In addition, the herbal medicine of the present invention has been used for herbal medicine and food for a long time, and herbal medicine extracts and separation compounds of the present invention extracted therefrom have no problems such as toxicity and side effects, and can be used safely even when used for a long time.

The compound produced by the above-mentioned method can be used for inhibiting tyrosinase enzyme activity, Elastase enzyme inhibition activity, celluli tyrosinase enzyme inhibition activity, melanin biosynthesis inhibition activity, pro-collagen activity, collagen and biosynthesis inhibitory activity, it was confirmed that the composition is useful as an external dermatological pharmaceutical composition or cosmetic composition for preventing and treating whitening.

The above-mentioned compound is characterized in that it comprises 0.1 to 50% by weight based on the total weight of the dermatological pharmaceutical composition.

The pharmaceutical composition includes a cream, a gel, a patch, a spray, an ointment, an alarm, a lotion, a liniment, a pasta or a cataplasmal formulation.

In addition, the cosmetic composition includes formulations of lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, and pack.

Accordingly, the present invention provides a dermatological pharmaceutical composition and a cosmetic composition for whitening treatment and prevention comprising the following production method and a compound obtained by the production method as an active ingredient.

In addition, the brambly seed of the present invention has been used for a long period of herbal medicine and food, and the extract of the present invention extracted therefrom has no problems such as toxicity and side effects and has proved to be a non-irritant sample in the skin patch test. Can be used with confidence.

The dermatological pharmaceutical composition containing the compound of the present invention can be used as a pharmaceutical composition in the form of external preparation for skin such as cream, gel, patch, spray, ointment, warning agent, lotion, liniment, pasta or cataplasm However, the present invention is not limited thereto.

The preferred dosage of the compound of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. However, for the desired effect, the compound of the present invention is preferably administered at 0.0001 to 100 mg / kg per day, preferably 0.001 to 10 mg / kg per day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The compounds of the present invention can be used variously in cosmetics and cleansers having a whitening-preventing effect.

Examples of products to which the present composition can be added include cosmetics such as lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, pack, cleansing, cleanser, soap, have.

The cosmetic composition of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular weight peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The water-soluble vitamin is not particularly limited as long as it can be compounded in cosmetics. Preferably, vitamin B, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, And their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbate-2-phosphate etc.) can also be added to water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

Usable vitamins include vitamins such as vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) , Derivatives thereof (such as palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbin, dl-alpha tocopherol acetic acid, dl-alpha tocopherol nicotinic acid vitamin E, dl-pantothenyl alcohol, D-pantothenyl alcohol, Ether, etc.) are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be any compound as long as it can be incorporated in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The seaweed extract may be any of those which can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red pepper extract, green algae extract and the like. Also, the algae extract may be colored guanine, arginic acid, Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic of the present invention may be blended with other essential ingredients, if necessary, in combination with the essential ingredients.

Examples of the compounding ingredients that may be added include organic solvents such as a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, a preservative, a bactericide, an antioxidant, a plant extract, a pH adjuster, A blood circulation accelerator, a cold agent, an antiperspirant agent, and purified water.

Examples of the oil retaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention and plant retention.

Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, Butyl isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, Trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid pentaerythritol tetra (2-ethylhexanoate) , Decyl caprylate, decyl laurate, hexyl laurate, myristate decyl, myristyl myristate, myristine monoethyl stearate, stearyl stearate, decyl oleate, ricinoleic acid tri , Isostearyl stearate, isostearyl stearate, isodecyl stearate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isopropyl stearate, isopropyl stearate, isopropyl stearate, -Hexyl stearate, stearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, Propyleneglycol propionate, propyleneglycol propionate, dicaproic acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyldodecyl Octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl lactate, octyldecyl lactate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisobutyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, But are not limited to, ethyl, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, But are not limited to, dioctyl sebacate, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitostearyl, Stearoyl hydroxystearic acid isostearyl, 12-stearoyl stearyl hydroxystearate, 12-stearo And monohydroxystearic acid and esters such as sostearyl.

Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.

Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Examples of animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, mead home oil, egg oil, , Canned wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.

Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. .

Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the nonionic surfactant include self emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE (Polyoxyethylene / polyoxypropylene) copolymer, POE.POP alkyl ether, polyether-modified silicone, polyether-modified silicone, polyoxyethylene-polyoxypropylene (POE) Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, Alkylsulfosuccinic acid salts, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphoric acid esters, and the like can be mentioned. have.

Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium chloride, Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino- p- (carbo-2'-ethylhexyl-1'- , 3,5-triazine, 2- (2- And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

Examples of the disinfectant include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, any of the above components may be blended within the range not to impair the objects and effects of the present invention, but it is preferably 0.01 to 5% by weight based on the total weight, Preferably 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients conventionally used in cosmetic compositions in addition to the above-mentioned compounds, for example, conventional additives such as stabilizers, solubilizers, vitamins, And a carrier.

The cosmetic composition of the present invention can be prepared into any formulation conventionally produced in the art, and examples thereof include emulsions, creams, lotions, packs, foundations, lotions, essences, and hair cosmetics.

Specifically, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, Packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

As described above, the compound isolated from the safflower extract of the present invention is useful as a tyrosinase enzyme inhibiting activity, an elastase enzyme inhibiting activity, a cellulous tyrosinase enzyme inhibiting activity, a melanin biosynthesis Inhibitory activity, pro-collagen biosynthesis inhibitory activity, and the like, the composition is useful as a whitening dermatological pharmaceutical composition or a cosmetic composition.

Brief Description of the Drawings Figure 1 is a graph showing the effect of a compound isolated from a safflower extract on cell viability in melanoma cells (B16F10) (the results are expressed as mean (mean) ± SD);
Figure 2 shows mushroom tyrosinase inhibitory activity of the compounds isolated from the extract of Safflower extract (the results are expressed as mean (mean) ± SD of triplicate);
3 shows the effect of a compound isolated from a safflower extract on tyrosinase-related protein expression in melanoma cells (B16F10), {(a) acacetin, (b) cosmosin, (c) N-feruloyl serotonin , and (d) N- (p-coumaroyl) serotonin, respectively. Cells were cultured for 48 hours in a medium containing 1 μM sample. The expression level of tyrosinase protein in each sample was normalized to the amount of β-actin. The mean values with different subscripts are significantly different. p <0.05)};
FIG. 4 is a graph showing the effect of a compound isolated from a safflower extract on the expression of TRP-1 protein in melanoma cells (B16F10), {(a) acacetin, (b) cosmosin, (c) N-feruloyl serotonin, and (d) N- (p-coumaroyl) serotonin. Cells were cultured for 48 hours in a medium containing 1 and 10 μM samples. The expression level of tyrosinase protein in each sample was normalized to the amount of β- The mean value with different subscripts is significantly different. p <0.05));
FIG. 5 is a graph showing the effect of a compound isolated from a safflower extract on the expression of TRP-2 protein in melanoma cells (B16F10) (acacetin, (b) cosmosin, (c) N-feruloyl serotonin, and (d) N- (p-coumaroyl) serotonin. Cells were cultured for 48 hours in a medium containing 1 and 10 μM samples. The expression level of tyrosinase protein in each sample was normalized to the amount of β- The mean value with different subscripts is significantly different. p < 0.05)).

Hereinafter, the present invention will be described in detail with reference to the following examples and experimental examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following Examples, Reference Examples and Experimental Examples.

Example 1. Safflower Extraction and Solvent Fraction

Dried safflower seeds were ground and 100% n- hexane was added and extracted at room temperature to remove fat. The above extraction procedure was repeated three times and 70% ethanol was added to the remaining residue, which was then extracted and filtered at room temperature. Under reduced pressure for 70% Ethanol extract obtained by carrying out the extraction operation, the above concentration into the following here Ethyl acetate (EtOAc) and the aqueous layer into the n -hexane fraction with n -hexane layer from the aqueous layer, and the fraction with EtOAc layer from the aqueous layer N- BuOH was added to the water layer and the water was separated into n- BuOH and water. During the above procedure, the EtOAc layer was concentrated under reduced pressure to obtain EtOAc extract (388.61 g). The EtOAc extract was assayed by HPLC to obtain an approximate view of the major materials. 200g of this extract was packed in a CHCl ₃ : MeOH (10: 1 to MeOH 100%) mixture and adsorbed on a column of Silicagel (230-400 mesh, Merck, Germany) to obtain 7 fractions. Among them, Fr.E1 and Fr.E5 obtained acacetin (7.74 mg) and cosmosiin (4.76 mg) respectively through recrystallization induction method using MeOH. CHCl Fr.E4 the total volume _{3: MeOH (5: 1 to} MeOH 100%) was charged into a mixture Silicagel (230 ~ 400 mesh, Merck , Germany) to obtain a five fraction was adsorbed to the column. Among them, Fr.E4.2 obtained N- feruloylserotonin (6.13 mg) single substance through recrystallization induction method using MeOH. The CHCl _{Fr.E4.3 3: MeOH (10: 1} to MeOH 100%) was charged into a mixture Silicagel (230 ~ 400 mesh, Merck , Germany) to obtain the eight fraction adsorbed to the column was. Is the amount of Fr.E4.3.4 _{CHCl 3: MeOH (15: 1} to MeOH 100%) was charged into a mixture Silicagel (230 ~ 400 mesh, Merck , Germany) to obtain the eight fraction was adsorbed to the column. Among them, N - ( p- coumaroyl) serotonin (15.33 mg) single substance was obtained from Fr.E4.3.4.7. (See Figures 1 and 2)

The physical properties of the individual compounds isolated from the extracts of the safflower extract were described below, and their properties were compared with the physical properties disclosed in the literature, and their structures were identified. (1980) Conjugated serotonins and phenolic constituents of safflower seed ( Carthamus tinctorius L.). A g ric ( 18) Sakamura, A., T erayama, Y., Kawakata, S., Ichihara, A. and Saito, B iol . Chem , ≪ / RTI > 44, 2951.)

Compound 1: Acacetin  ( Acacetin )

Appearance: white amorphous powder

Molecular Weight: 270.2369

^{1 H-NMR (500MHz, DMSO-} d 6 + D 2 O): δ 6.86 (1H, H-3), 6.20 (1H, d, J = 2Hz, H-6,), 6.50 (1H, d, J = 2Hz, H-8), 8.03 (2H, d, J = 9Hz, H-2 ', 6'), 7.10 (2H, d, J = 9Hz, H-3 ', 5')

¹³ C-NMR (500 MHz, DMSO- d ₆ + D ₂ O):? 163.3 (C-2), 103.6 (C-3), 181.8 6), 164.3 (C-7), 94.1 (C-8), 161.5 (C-9), 103.8 114.6 (C-3 ', 5 '), 162.3 (C-4 '), 55.6 (-OCH 3)

Compound 2: Cosmosin ( Cosmosin )

Appearance: white amorphous powder

Molecular Weight: 432.3775

^{1 H-NMR (500MHz, DMSO-} d 6 + D 2 O): δ 6.82 (1H, H-3), 6.43 (1H, H-6), 6.86 (1H, d, J = 2.5Hz, H-8 ), 7.94 (2H, dd, J = 9 Hz, 2.5 Hz, H-2 ', 6'), 6.93 (2H, dd, J = 9 Hz, 2.5 Hz, H- d, J = 7.5 Hz, glucose-H)

^{13 C-NMR (500MHz, DMSO-} d 6 + D 2 O): δ 164.3 (C-2), 103.1 (C-3), 182.0 (C-4), 161.1 (C-5), 99.5 (C- 6), 163.0 (C-7), 94.9 (C-8), 157.0 (C-9), 105.4 C-4 '), 99.9, 77.2, 60.6, 69.6, 76.4, 73.1 (glucose-C)

Compound 3: N - ferulocortonin ( feruloylserotonin )

Appearance: white amorphous powder

Molecular Weight: 352.3838

^{1 H-NMR (500MHz, Methanol} ): δ 6.93 (1H, H-2), 6.88 (1H, d, J = 2Hz, H-4), 6.58 (1H, dd, J = 2.5Hz, 8.5Hz, H -6), 7.07 (1H, d , J = 8.5Hz, H-7), 2.84 (2H, t, J = 7Hz, H-10), 3.48 (2H, t, J = 7Hz, H-11), 7.01 (1H, d, J = 1.5Hz, H-2 '), 6.69 (1H, d, J = 8Hz, H-5'), 6.91 (1H, d, J = 1.5Hz, H-6 '), 7.35 (1H, d, J = 15.5Hz, H-7 '), 7.32 (1H, d, J = 15.5Hz, H-8')

^{13 C-NMR (500MHz, Methanol} ): δ 124.4 (C-2), 112.8 (C-3), 103.7 (C-4), 151.3 (C-5), 112.5 (C-6), 112.7 (C- (C-1), 111.7 (C-2), 149.4 (C-11), 133.6 C-3 '), 149.9 (C-4'), 116.6 (C-5 '), 123.4 '), 56.4 (-OCH ₃₎

Compound 4: N - ( p - Kumaroto ) Serotonin  ( N - ( p - coumaroyl ) serotonin )

Appearance: white amorphous powder

Molecular Weight: 322.3578

^{1 H-NMR (500MHz, Methanol} ): δ 6.91 (1H, H-2), 6.86 (1H, d, H-4), 6.56 (1H, dd, J = 2.5Hz, 8.5Hz, H-6), 7.05 (1H, dd, J = 3.0Hz, 8.5Hz, H-7), 2.82 (2H, t, J = 7Hz, H-10), 3.46 (2H, t, J = 7Hz, H-11), 7.28 (1H, d, J = 5.5Hz , H-2 '), 6.67 (1H, d, J = 5.5Hz, H-3'), 6.67 (1H, d, J = 5.5Hz, H-5 '), 7.28 (1H, d, J = 5.5Hz, H-6 '), 7.34 (1H, dd, J = 2.5Hz, 15.5Hz, H-7'), 6.28 (1H, dd, J = 3.5Hz, 15.5Hz , H-8 ')

^{13 C-NMR (500MHz, Methanol} ): δ 124.6 (C-2), 112.5 (C-3), 103.7 (C-4), 151.3 (C-5), 112.6 (C-6), 112.8 (C- (C-11), 127.9 (C-1), 130.7 (C-2), 116.8 (C-10) 3 '), 160.6 (C-4'), 116.8 (C-5 '), 130.7 (C-6'), 141.9 ')

Experimental Example  One. MTT assay Of cell survival

In order to test the cell viability by MTT assay of the samples obtained in the examples, Carmichael's method disclosed in the literature was applied as follows (Carmichael J, Degraff WG, Gadzar AF 1987. Evaluation of a tetrazolium based semiautomated colorimetric assay : Assessment of chemosensitivity testing. Cancer Res 477: 936-942 ..)

1-1. Cell culture

Cells of mouse black (B16F10, Manassas, Va.) Cells were cultured in the presence or absence of cells of the spinal cord of neurons cultured in the spinal cord of neurons cultured in spinal cord neuron cultures, J. Neurosci Res. 37: 62-70 (1994), Michikawa et al. ( Michikawa M, Lim KT, McLarnon JG, according to the process of 19) was separated and then diluted with process a 0.25% trypsin solution to the culture cells following DuLbeco's modified eagle's medium (DMEM ) 10% fetal bovine serum (FBS) in the culture medium and 1% penicillin / streptomycin (100U / mL ) Was added and cultured at 37 ° C in a 5% CO ₂ incubator.

1-2. MTT assay Of cell viability

Cell viability was measured by the method of Carmichael et al. ( Carmichael J, Degraff WG, Gadzar AF 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: Cancer Res 477: 936-942. 20) . Melanoma (B16F10) was dispensed into a 96-well plate at a concentration of 5 × 10 ⁴ cells / well (0.18 mL), and 0.02 mL of each sample was added to the wells and incubated at 37 ° C in a 5% CO ₂ incubator for 48 hours. In the control group, the same amount of distilled water as that of the sample was added and the cells were cultured under the same conditions. After adding 0.02 mL of MTT solution (5 mg / mL) and incubating for 4 hours, the culture solution was removed, and 0.15 mL of DMSO: EtOH (1: 1) was added to each well and reacted at room temperature for 30 minutes. Absorbance was measured at 550 nm. Cytotoxicity measurement was expressed by the absorbance reduction ratio of the group of addition of the sample solution and the group of no addition of the following formula (1).

The survival rate of melanoma cells was confirmed by MTT assay as shown in Fig. (p-Coumaroyl) serotonin showed a low cytotoxicity with a survival rate of 80% at concentrations of 1 and 10 μM, and 50% at 50 and 100 μM, respectively. The survival rate was as follows, indicating a strong cytotoxicity rate. Tyrosinase activity, melanin biosynthesis and whitening signal transduction factors in melanoma cells (B16F10) were measured at concentrations of 1 and 10 μM, which were close to 100% in survival rate.

Experimental Example  2. Whitening activity experiment

2-1. Tyrosinase  Inhibitory activity

In order to test the tyrosinase inhibitory activity of the samples obtained in the examples, the following experiment was conducted by applying the method of Yagi et al. ( 21 ) to Yagi AT, Kanbara T, Morinobu N. The effect of tyrosinase inhibition aloe, Planta Med., 3981: 517-519 (1986))

To the reaction mixture, 0.2 mL of mushroom tyrosinase (110 U / mL) was added to a mixture of 0.2 mL of the substrate solution in which 10 mM L-DOPA was dissolved in 0.5 mL of 0.175 M sodium phosphate buffer (pH 6.8) and 0.1 mL of the sample solution and the mixture was reacted at 25 ° C. for 2 minutes And the DOPA chrome produced in the reaction solution is measured at 475 nm. The tyrosinase inhibitory activity was represented by the absorbance reduction rate of the sample solution of the following formula (2) and the non-added sample.

As a result of this experiment, the results of measurement of inhibitory activity of tyrosinase derived from mushroom are shown in FIG. In this study, tyrosinase activity was reduced at a concentration of 1,000 μM in acacetin, cosmosin, N-feruloyl serotonin and N- (p-Coumaroyl) serotonin. In particular, acacetin exhibited the best efficacy with a sensitivity of 59.2% at 1,000 μM.

2-2. Expression of whitening-related proteins

In order to test the effect of the samples obtained in the examples on the expression of the whitening-related protein, the method of measuring the expression of the whitening-related protein through the Western blot method described in the literature was applied as follows.

B16F10 melanoma cells were divided into 2 × 10 ⁴ cells / well in a 100-mm tissue culture dish and cultured for 24 hours to examine whitening factors MITF, TRP-1, TRP-2 and tyrosinase activity . After the medium was removed, the sample was exchanged with the treated medium for various concentrations, cultured for 48 hours, and then washed with PBS. Cells were lysed by adding 100 μl of lysis buffer (Pierce, Rockford, IL) and centrifuged (12,000 rpm, 4 ° C, 20 min) to remove cell membrane components. The proteins obtained by centrifugation were quantitated by bradford assay, and 60 μl of the protein was electrophoresed using 10% SDS-PAGE (Pierce, Rockford, Ill.) And transferred to a PVDF membrane to inhibit nonspecific binding of the antibody And then transferred at 30 to 40 V for 2 hours or more. The separated proteins were transferred to PVDF membrane (Hercules, CA, USA) and incubated in blocking buffer (5% skim milk in TBST) for 1 hour at room temperature. Each of the primary antibodies was diluted 1: 1000 and then over-night at 4 ° C. The cells were washed three times with TBST (Tris-Buffered Saline and Tween, Santa Cruz, CA) Each secondary antibody of rabbit IgG HRP (Santa Cruz, Calif.) was diluted 1: 1,000 and incubated at room temperature for 2 hours. After washing three times, bands were identified and quantified using a LAS 4000 image analyzer (Fugifilm life science, Tokyo, Japan).

In order to investigate the effect of the present invention on tyrosinase, an enzyme involved in the synthesis of melanin, B16F10 mouse melanoma cells were treated with 1, 10 μM of TRP-1, TRP-2, tyrosinase protein Were confirmed by western blotting. Β-actin (Santa Cruz, CA), a house keeping gene with little difference in expression level, was used as a positive control.

As shown in Figs. 3 to 5 (= Fig. 3 to 5), the protein expression of each transcription factor was increased in the B16F10 group not treated with acacetin, cosmosin, N-feruloyl serotonin and N- (p- Coumaroyl) serotonin, The expression of MITF, TRP-1, TRP-2 and tyrosinase protein in the B16F10 group treated with 1, 10 μM of acacetin, cosmosin, N-feruloyl serotonin and N- (p- Coumaroyl) Respectively. These results suggest that acacetin, cosmosin, N-feruloyl serotonin and N- (p-Coumaroyl) serotonin may inhibit melanogenesis by inhibiting the expression of MITF, TRP-1, TRP-2 and tyrosinase do.

Hereinafter, formulations of cream, massage cream, lotion, skin lotion, essence, pack, and cleansing foam are exemplified as the formulation examples of the present invention, but the formulations including the cosmetic composition of the present invention are not limited thereto.

Formulation example  One. hair Tonic  Produce

Acacetin -------------------------------- 1%

Ethanol ----------------------------------------- 55.0%

Castor Freedom ---------------------------------------- 5.0%

Glycerin ---------------------------------------- 3.0%

Pyrrotonamine -------------------------------------- 0.1%

Fragrance, coloring --------------------------------------

Purified water ------------------------------------------ Remnant

Total -------------------------------------------- 100.0%

Formulation example  2. hair  Manufacture of lotions

Cosmosi (cosmosiin) --------------------------- 1.0%

Cetostearyl alcohol ------------------------------ 2.0%

Stearyl triethylammonium chloride - 2.0%

Hydroxyethylcellulose 0.5%

0.0 > 0.1% < / RTI >

Spices, coloring -------------------------------------- 0.5%

Purified water ----------------------------------------- Remainder

Total -------------------------------------------- 100.0%

Formulation example  3. hair  Manufacture of soap

N - Feruloylserotonin ------------- 0.1%

Titanium Dioxide -------------------------------------- 0.2%

Polyethylene glycol -------------------------------- 0.8%

Glycerin ---------------------------------------- 0.5%

Ethylenediamine tetraacetic acid - 0.05%

Sodium ------------------------------------------ 1.0%

Pigment, soap fragrance ------------------------------------

Makeup soap base (moisture content 13%, parts by weight) ---------------- Remainder

Total -------------------------------------------- 100.0%

Formulation Example  1. Cream composition

The oil phase and water phase are heated to 75 ° C and cooled to room temperature.

Formulation Example  2. Massage Cream  Composition

The oil phase and water phase are mixed by heating at 75 DEG C and then cooled to room temperature.

Formulation Example  3. lotion composition

The oil phase and water phase are mixed and emulsified by heating at 75 ° C and then cooled to room temperature.

Formulation Example  4. Skin lotion composition

The water phase and the ethanol phase are respectively prepared and mixed and then filtered.

Formulation Example  5. Essence composition

The water phase and the ethanol phase are respectively prepared and mixed and then filtered.

Formulation Example  6. Pack composition

The water phase and the ethanol phase are dispersively dissolved and mixed, and then cooled to room temperature.

Formulation Example  7. Cleansing Foam  Composition

The water phase and the oil phase are dispersed and dissolved, mixed and sieved, and then cooled to room temperature.

Although the compositional ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the compounding ratio according to the regional or national preference such as the demand class, the demanding country, and the use purpose.

Claims (5)

A whitening skin containing a compound selected from acacetin, cosmosiin, N - feruloylserotonin, or N - ( p - coumaroyl) serotonin isolated from a safflower extract as an active ingredient ≪ / RTI > The method according to claim 1,
Wherein the extract is at least one solvent selected from the group consisting of water, ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, butylene glycol, propylene glycol, hydrolyzed butylene glycol, Composition. The method according to claim 1,
Wherein the pharmaceutical composition is a cream, a gel, a patch, a spray, an ointment, a warning agent, a lotion, a liniment, a pasta or a catarcoma. A whitening cosmetic composition containing a compound selected from acacetin, cosmosiin, N - feruloylserotonin, or N - ( p - coumaroyl) serotonin isolated from a safflower extract as an active ingredient Composition. The method of claim 4, wherein
The cosmetic composition is a formulation of lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, or pack.

Images