KR20140097728A - Wound dressing foam containing cuttlefish bone powder and method for preparing the same - Google Patents

Abstract

The present invention relates to wound dressing foam containing cuttlefish bone powder and a method to prepare the same. According to the present invention, the wound dressing foam containing cuttlefish bone powder has excellent absorption property compared to a conventional wound dressing foam, and has an effect of quickly treating wounds such as a cut by having excellent hemostasis and scar removal effects.

Description

TECHNICAL FIELD The present invention relates to a wound dressing preparation containing cuttlefish bone powder and a method for preparing the same,

The present invention relates to a wound dressing for treating wounds containing cuttlefish bone powder and a method for producing the dressing.

The cuttlefish bone is an endoskeleton of Sepia officinalis L. It is composed of spongy and cementous materials and contains a large amount of calcium components and is used as a raw material for food birds and toothpastes such as parrots (Kim et al ., 2000). In addition, squid bone has been conventionally used as a hemostatic agent and a wound closure agent, but in recent years, it has excellent biocompatibility as a naturally derived ingredient, and research for application to an artificial bone substitute material or a biosensor has been actively carried out.

The most studied field of tissue engineering is the production of medical scaffolds using aragonite (CaCO ₃ ), which is a major component of bone squid bones. Hydroxyapatite derived from hydrothermal extraction of aragonite apatite, HAP) have higher porosity characteristics, which can maximize the efficiency of the function of inducing bone cell invasion or angiogenesis (Ivankovic et al., 2008). In particular, the size of the intrinsic pore of squid bones is a suitable size for inducing physical activity of tissue growth and angiogenesis, and provides a suitable environment for promoting osteoblast proliferation and differentiation (Rocha et al. , 2005). In addition, it has been confirmed that the squid bones promote cell proliferation and enhance cell adhesion through mesenchymal stem cell differentiation experiments, and it is confirmed that the squid bone cell differentiation and application possibility as proliferation promoting medium and bone regeneration substance (Kim et al., 2011). According to recent research results, HAP is very similar to human bone and its constituents and microstructure, so it can be used as an alternative to artificial bone substitute. Medical scaffolds have been widely used (Kannan et al., 2006).

In the field of food or fermentation analysis, squid bones are widely used in biosensor applications for quantitative analysis of sugars. In the previous studies, glucose - glucose oxidation - reduction reaction using gold nanoparticle catalyst was used for quantitative analysis of glucose, but the measurement efficiency was low due to the dispersion of gold nanoparticles in the measurement solution. However, by attaching gold nanoparticles densely in the porous structure of the cuttlefish bone, induction of measurement efficiency and resolution improved the efficiency of indirect quantitative analysis of glucose and other saccharides (Xu et al., 2009).

In addition, the antioxidant properties of cuttlefish bone are widely used in the medical and cosmetic industries. Generating the other hand, glutathione and peroxide decomposing enzyme is everywhere (ENA) activity mineral A extract solution produced by the purification process of the cuttlefish bone to reduce the generation and lipid peroxidation, induction of CYP2E1 cytochrome of active oxygen is increased by by CCl ₄ Induced oxidative stress and hepatotoxicity (Hong et al., 2011). However, there have been no studies to develop a hydrocolloid membrane and evaluate its efficacy to treat wounds.

The dressing agent (hydrocolloid membrane) protects the damaged skin and treats the wound. It connects the epithelium of the skin surface with the underlying connective tissue, protects the wound from infection, maintains proper moisture, , And to facilitate the regeneration or production of epithelial tissue (Choi, JH, 2012). However, in the past, such a dressing agent was simply used to dry a wound using a general cotton or a cotton wool. However, in such a cotton wool dressing, it is impossible to keep the wool wet. When there is excessive exudate, it can not be absorbed. It is wide and can not prevent the invasion of bacteria. In addition, new tissue of the wound can grow into the cotton wool, and when the dressing is stuck with the wound, it gives trauma to the wound, thus the wound healing becomes late and the pain during dressing is also caused. It only covers the wound, but does not functionally promote wound healing (Han and Youn, 2011). However, since 1962 Winter announced that faster epithelium formation occurs under humid conditions, wound dressing under wet conditions has begun to be studied (Choi, Jinhyun, 2012). Therefore, a number of wet dressing materials have been developed to make the environment more suitable for the wound area recently. Major products on the market include Ildong Pharm. Co., and Boryung Pharm. Co.

The hydrocolloid used as the wet dressing agent is pectin, gelatin, and carboxymethylcellulose, and forms hypoxia of the wound as a main component to stimulate neovascularization. When the exudate secreted from the wound contacts with the hydrocolloid, To inhibit the formation of blood clots. Therefore, hydrocolloid dressing is increasingly being used as a useful agent to properly absorb exudates, prevent water loss, and prevent secondary bacterial infections, thereby helping wound healing (Jung So-young).

Therefore, there is a desperate need for research to develop a wound dressing for wound healing which is excellent in absorbability and excellent in hemostatic and scar removal effects.

The inventors of the present invention have discovered that when treating a dressing agent for treating wounds such as wounds more quickly, the effect of treating wounds is improved when manufacturing a dressing agent including squid bone powder, and completed the present invention .

Accordingly, the present invention is to provide a wound dressing agent containing cuttlefish bone powder and a method for producing the same.

The present invention provides a dressing for wound healing comprising squid bone powder and a method for producing the same.

The wound dressing preparation containing the cuttlefish bone powder according to the present invention is excellent in absorbability compared with the conventional dressing dressings for wound healing and has excellent hemostatic and scar removing effects and has an effect of treating wounds such as wounds quickly .

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the results of weight changes of animals during wound healing. FIG.
Fig. 2 is a photograph showing changes in the size of wound of an animal during wound healing.
FIG. 3 is a graph showing changes in the size of wound of an animal during wound healing.
Fig. 4 is a diagram showing the tissue result for analyzing the effect of the mixed squid bone colloid membrane on the change of the wound tissue.

The present invention provides a wound dressing for treating wounds containing cuttlefish bone powder.

In addition,

1) adding a petroleum resin to the reactor to dissolve it, adding rubber and tetrakismethane, and stirring to prepare a prepolymer;

2) adding cellulose gel and liquid paraffin to the prepolymer and stirring to prepare a composite; And

3) preparing a cuttlefish bone composite by adding powdered squid bone powder to the composite, and then coating the cuttlefish bone composite on a release paper to prepare a dressing;

And a method for manufacturing a dressing for wound healing.

Hereinafter, the present invention will be described in detail.

The dressing for dressing according to the present invention is characterized in that the cuttlefish bone powder contains 15 to 45% of the total volume of the dressing agent.

The dressing agent may include, but is not limited to, petroleum resin, rubber, tetrakismethane, cellulose gum, liquid paraffin, and squid bone powder.

The preparation of the dressing agent may include a material commonly used in the art and a squid bone powder. For example, a biocompatible polymer such as chitosan, polyethylene glycol, polypropylene glycol, glycerin, polyvinyl alcohol, or polypyrrolidone, and may also be prepared by including squid bone powder.

The method of manufacturing a dressing for wound healing according to the present invention will be described step by step as follows.

In the method for producing a dressing agent of the present invention,

Step 1) is a step of preparing a prepolymer. The prepolymer is prepared by dissolving 100 to 300 g of a petroleum resin in a reactor, adding 100 to 300 g of rubber and 2 to 10 g of tetrakismethane, and stirring to prepare a prepolymer.

Step 2) is a step of preparing a composite. 100 to 300 g of cellulose gum and 30 to 150 g of liquid paraffin are added to the prepolymer, followed by stirring to prepare a composite.

Step 3) is a step of preparing a dressing agent. The cuttlefish bone powder is added to the complex by applying the powdered cuttlefish bone powder corresponding to 15 to 45% of the volume of the complex, and the cuttlefish bone composite is coated on the release paper To prepare a dressing agent.

The wound dressing prepared by the above method had a salinity concentration of 0.06 ± 0.004%, a resistance value of 4.91 ± 0.18, and an electrical conductivity of 506 ± 17.37, and had a neutral pH value of 8.27 ± 0.02 and was used as a wound healing agent And the hygroscopicity of the distilled water is superior to that of the other dressing agent, and thus, it has an excellent effect on wound healing.

As described above, the wound dressing preparation containing the cuttlefish bone powder according to the present invention is superior in absorbability compared to the conventional wound dressing dressings, and has excellent hemostatic and scar removing effects, There is an effect of curing.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the examples.

Example 1. Preparation of mixed squid bone hydrocolloid membrane

1-1. Preparation of cuttlefish bone powder

The cuttlefish bones were purchased from Burner World Co., Ltd., and were precipitated in distilled water for 72 hours in order to remove the salt remaining in the cuttlefish bone. To remove the oily components (oil component), industrial methanol (90% Lt; / RTI > The precipitated squid bones were washed with distilled water, naturally dried at room temperature (20 ° C) for 24 hours, and then dried in an oven (80 ° C) for 5 hours. The completely dried squid bones were first crushed using a spatula and then ground by a pulverizer (DE / A10, IKA-WERKE Co, Germany). Powdered cuttlefish bones were stored in a -20 ° C freezer to prevent denaturation of organic matter contained in the bone structure.

1-2. Preparation of mixed squid bone hydrocolloid membrane

After 200 g of a petroleum resin was added to a hydrocolloid reactor (Yukin Chemical Co., Ltd.) and sufficiently dissolved, 200 g of rubber and 6 g of tetrakis methane were added and stirred for 1 hour and 30 minutes to prepare a prepolymer. 210 g of cellulose wool and 70 g of liquid paraffin were added to the prepolymer, followed by sufficiently dispersing by stirring to prepare a viscous composite. The squid bone powder corresponding to 15% and 30% of the volume of the composite was dispersed, Bone mixed hydrocolloid complexes were prepared. The prepared mixed squid bone mixed hydrocolloid composite was coated on a silicon-coated release paper with a knife-coater to a thickness of 450 탆, and the top of the coated squid bone was covered with a polyurethane film to finally obtain a mixed squid bone hydrocolloid (Dressing agent) was prepared.

Experimental Example 1. Biochemical characterization of bone squid powder

In order to analyze the biochemical characteristics of squid bone powder, salinity, electrical conductivity and pH of cuttlefish bone powder were measured. 2 g of squid bone powder was dispersed in 60 ml of distilled water according to the concentrations indicated in the appropriate standards of the apparatus, and then the salinity, electrical conductivity and pH were measured after stirring for 24 hours. The salinity and electrical conductivity were measured using a salt concentration meter (EC-400L, Istek), and the resistance and pH were measured using a pH meter (Mettler D20-K, same as Science).

Table 1 shows the salt concentration of the powder of squid bone powder.

Classification Salinity Concentration (%) 0.1% NaCl solution 1.13 + - 0.12 1% NaCl solution 10.5 ± 0.21 Cuttlefish bone powder dispersion 0.06 ± 0.004

As shown in Table 1, the salt concentration of the squid bone powder dispersion was about 0.06%, which was 19 times lower than 1.13% measured in the 0.1% NaCl solution, and about 175 times higher than the 10.5% measured in the 1% NaCl solution Respectively. Therefore, this result shows that the dispersion of squid bone powder in salted squid bone powder is close to zero and is suitable as a wound healing agent.

Electrical conductivity and resistance are commonly used as a measure of relatively pure water. The more ions are in the water, the higher the current, the higher the conductivity, and vice versa (Kim et al., 1996). Therefore, in order to analyze the content of foreign matter in the bone squid bone dispersion, resistance and electrical conductivity of the bone squid bone dispersion were measured. The results are shown in Table 2 below.

Classification Resistance value (Ω / cm, E ⁺⁰² ) Electrical Conductivity (ms / cm) Distilled water 1.76 + 0.08 4.24 + 0.14 tap water 7.24 ± 0.05 83.58 ± 0.51 Cuttlefish bone powder dispersion 4.91 + 0.18 506 ± 17.37

As shown in the above Table 2, the resistance value of the bone squid bone dispersion was lower than tap water (7.24? / Cm) and 4.91? / Cm higher than distilled water (1.76? / Cm). However, the electrical conductivity increased to 506 ms / cm, which is significantly higher than tap water (83.58) and distilled water (4.24), as opposed to the resistance value. These results suggest that the dispersion of squid bone powder has a low content of foreign substances and is less likely to cause toxicity or infectious disease in wound healing.

In addition, the pH measurement results of the dispersion of squid bone powder are shown in Table 3 below.

Classification pH Distilled water 7.63 + - 0.76 Cuttlefish bone powder dispersion 8.27 ± 0.02

As shown in Table 3 above, the pH of the squid bone powder dispersion was slightly higher than that of distilled water (7.63), but was not significant and showed a neutral value. These properties indicate that there is a neutrality characteristic suitable for application as a wound healing agent.

Experimental Example 2. Hygroscopicity analysis of distilled water of mixed squid bone hydrocolloid membrane

In order to analyze the hygroscopicity of mixed squid bone hydrocolloid membranes, 0.1 g of each of the hydrolyzate, hydrocolloid membrane, and mixed squid bone hydrocolloid membrane was precipitated in distilled water for 5 minutes, 10 minutes, 30 minutes, 1 hour intervals These changes were measured using an electronic balance and the weight gain per unit volume was calculated.

Excellent wound healing materials are: 1) Prevention of bacterial infection, 2) Easy removal of exudates (dirt), 3) Removal of dressing without damaging newly formed skin tissue and epithelialized tissue, 4) Maintaining the temperature close to the body temperature is essential (Han and You, 2011). Among these properties, the property required to remove the exudates is absorbency. The hydrocolloid has a structure characteristic of porosity and can absorb and retain a large amount of water, so that it is very easy to remove exudates (Choi, JH, 2011). Wound healing agents with excellent absorbability can not only prevent blood clots but also help to heal wounds. In addition, factors necessary for skin regeneration and wound healing, such as neutrophils, leukocytes, and macrophages present in exudates, (Jung, Hong, 2004). In this study,

In order to compare the absorbency of the mixed squid bone hydrocolloid membrane used in the medical band and the squid bone mixed hydrocolloid membrane used in the present invention, the absorbance was calculated by measuring the weight at 5 minutes, 10 minutes, 30 minutes and 1 hour. The results are shown in Table 4 below.

Classification Weight / Volume (g / cm ³ ) 0 min 5 min 10 min 30 min 60 min cotton wool 0.4 0.82 (+/- 0.05) 0.87 (+/- 0.05) 0.89 (0.03) 0.98 (0.07) Hydrocolloid One 2.23 (+ - 0.40) 2.44 (+/- 0.20) 2.39 (+/- 0.26) 2.39 (+/- 0.16) Cuttlefish bone mixed hydrocolloid (15%) One 2.12 (+/- 0.17) 2.01 (+/- 0.16) 2.33 (+ - 0.23) 2.35 (+/- 0.44) Cuttlefish bone mixed hydrocolloid (30%) One 2.4 (± 0.10) 2.52 (+ - 0.36) 2.54 (0.02) 2.48 (+ -0.22)

As shown in Table 4, the absorbency of the cotton wool blend hydrocolloid membrane was about 5 minutes and 2.5 times higher than that of the sample after 5 minutes. In addition, the 15% mixed hydrocolloid membrane showed a similar absorbance to the hydrocolloid membrane, but the 30% mixed hydrocolloid membrane of the squid bone showed significantly higher absorbance than the hydrocolloid membrane. Therefore, this result indicates that the mixed squid bone hydrocolloid membrane has a high absorbency similar to that of the cotton wool so that it can be utilized as an easy material for absorbing the leached material generated from the wound.

Experimental Example 3. Animal Experiment to Evaluate Wound Recovery Ability

SD rats (6-week-old male) were purchased from Osan, Korea and fed with radiation (Purina, Missouri, USA) ) Were housed in a clean laboratory animal center at Pusan National University (temperature: 22 ℃, relative humidity: 50%). This study was approved by the PNU-IACAU (Pusan National University) Animal Experimental Ethics Committee (PNU2012-0181).

The experimental group consisted of 4 groups of 7 rats per group, totaling 28 rats. First, 5 cm ² of hair was removed from the dorsal side of the rat, and a round wound of about 10 mm in diameter was made by biopsy. The first group was treated with sterile cotton wool only (wound dressing group) after wounding, and the second group treated with hydrocolloid film on wound (hydrocolloid membrane group). The third group treated hydrocolloid membranes mixed with 15% of squid bones (15% mixed squid bone hydrocolloid membrane group) and the last group treated hydrocolloid membranes mixed with 30% of squid bone (10%) Squid bone 30% mixed hydrocolloid membrane group). The wound area was observed daily for 10 days, and at the same time, the cotton swab and the three hydrocolloid membranes were replaced daily.

In order to observe the wound healing effects of mixed squid bone hydrocolloid membranes, wound tissue was treated with mixed squid bone hydrocolloid membrane for 10 days to observe the wound healing.

First, the body weight change of an animal is shown in FIG. 1, and the size change of the wound is shown in FIG. 2 and FIG.

As shown in Fig. 1, the change in body weight was slightly different by about 10 days after wounding but there was no significant difference in the four groups.

In addition, as shown in Figs. 2 and 3, the size of the wounds was very similarly decreased with the lapse of time in the group treated with the cotton wool, hydrocolloid membrane, and 15% mixed hydrocolloid membrane of squid bone. However, in the group treated with 30% mixed hydrocolloid membrane of cuttlefish bone, the size of wound was significantly decreased with time as compared with the group treated with hydrocolloid membrane. Therefore, these results suggest that 30% mixed hydrocolloid membrane of cuttlefish bone promotes recovery and inhibits scar formation in wound healing.

Experimental Example 4. Analysis of tissue

Immediately after autopsy, the wound tissues were fixed in 10% formalin solution for 72 hours, cut into appropriate size, placed in a cassette, and fixed with a tissue processor (Thermo Shandon Ltd./Thermo Fisher Scientific, England) . Immobilized tissues were impregnated using a paraffin embedding station (Leica Microsystems Nussloch GMBH, Germany) and then resected using a Rotary Microtome (Leica Microsystems Nussloch GMBH, Germany). The dissected tissue was immersed in xylenes three times for 3 minutes, 100% for 2 minutes each for 1 minute, 95%, 80% and 70% alcohol for 1 minute 30 seconds, washed with water for 1 minute, and hematoxylin, For 1 min, 10% scott's tap water for 1 min, and eosin for 3 min. The dyed tissues were dehydrated again in the order of 70, 80, 95, and 100% alcohol, and finally treated with xylenes three times for 3 minutes each. The tissue was then fixed on a slide using kanadabalsam and xylene-fixed fasteners and then dried. The effects of mixed colloid membranes of cuttlefish bone on the changes of wound tissues were analyzed using a microscope. The results are shown in Fig.

As shown in Fig. 4, scabs were observed in the vehicle treatment group in a large amount, but in the hydrocolloid or osseous mixed hydrocolloid treatment group, the scab was greatly reduced. In particular, most of the scabs disappeared in the miscible hydrocolloid treated group. The length of scar tissue was significantly shorter than that of the control group, and the group with hydrocolloid treatment was the shortest. The epidermis (epithelium) was regenerated in the vehicle group and the hydrocolloid membrane treatment group, whereas the ossicle mixed hydrocolloid treatment group was completely regenerated. However, blood vessel regeneration and hair follicle production were completely regenerated only in the mixed hydrocolloid treatment group of the ossicular bone 30, and only a small part of the blood vessels were regenerated in the remaining group. Therefore, this result suggests that the mixed colloidal hydrocolloid membrane of the oval bone 30 has the effect of shortening the recovery time of wound tissue and suppressing scar formation.

Claims (4)

Dressing agent for treating wounds containing cuttlefish bone powder. The dressing according to claim 1, wherein the squid bone powder comprises 15 to 45% of the total volume of the dressing. The dressing according to claim 1, wherein the dressing agent comprises petroleum resin, rubber, tetrakismethane, cellulose gum, liquid paraffin, and squid bone powder. 1) adding a petroleum resin to the reactor to dissolve it, adding rubber and tetrakismethane, and stirring to prepare a prepolymer;
2) adding cellulose gel and liquid paraffin to the prepolymer and stirring to prepare a composite; And
3) preparing a cuttlefish bone composite by adding powdered squid bone powder to the composite, and then coating the cuttlefish bone composite on a release paper to prepare a dressing;
≪ / RTI >

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