KR20140095599A - Producing Method of Bio-Ethanol by using sweet sorghum juice - Google Patents

Abstract

The present invention relates to a method for manufacturing bioethanol, which comprises the steps of (a) preparing a medium by concentrating sweet sorghum juice; (b) obtaining a culture liquid by adding yeast to the medium to culture a starter; (c) separating the culture liquid into liquid and solid, collecting microbial cells obtained from the solid, and putting the microbial cells into a fermentation tank to obtain a fermented solution including ethanol; and (d) producing ethanol by conducting a process of distilling and dehydrating the fermented solution.

Description

Technical Field [0001] The present invention relates to a method for producing bioethanol using a single water solution,

The present invention relates to a method for producing bioethanol by using a single-watered juice as a culture medium for seed culture necessary for an ethanol production fermentation process.

As the global exhaustion of fossil fuels and concerns over environmental pollution increase, the concept of renewable and alternative energy that produces stable and sustainable energy is becoming a hot topic. As part of such alternative energy development, technology for producing biofuels, for example, bioethanol, from biomass resources has received the most attention.

Biomass, which is a raw material for bioethanol, contains the first generation including lignocellular (sugarcane, beet), starchy (corn, potato, sweet potato, etc.), and lignocelluloses (Wood, rice straw, waste paper, etc.) that are made of wood. Recently, development of biofuel using marine algae as a raw material as a third generation bioethanol fuel is underway.

However, among the above-mentioned raw materials of bioethanol, the first generation grain system has problems such as competing with food and livestock feed, saturation of cultivation area, etc. In the case of the second generation woody system, in order to remove lignin There is a disadvantage that it is required to undergo a complicated and costly pretreatment process. Thus, there is a problem in that raw materials for producing bioethanol are not easily supplied only by the respective raw materials mentioned above. In addition, when the existing bioethanol production processes of the first, second, and third generations are respectively used, there is a problem in that there is no alternative to increase the price of bioethanol when the price of the raw material increases because it is highly dependent on a single raw material.

Therefore, in order to replace the fuel, it is necessary to secure a large amount of raw materials and develop a low-cost, high-efficiency production technology.

KR 10-2004-0020902 (application number)

The present invention intends to increase the ethanol production efficiency by combining the first-generation bioethanol feedstock with the second generation bioethanol production process.

(A) preparing a culture medium by concentrating a single-watered juice; (b) culturing the seed culture by adding yeast to the culture medium to obtain a culture solution; (c) separating the culture solution into a liquid phase and a solid phase, recovering the cells obtained from the solid phase, and introducing the cells into a fermentation tank to obtain a fermentation broth containing ethanol; And (d) subjecting the fermentation broth to distillation and dehydration to produce ethanol; And a method for producing bioethanol.

The present invention uses a single water juice as a medium to simplify the process than adding a conventional (YP) medium and to separate the microorganisms into solid and liquid to continuously use the microorganisms as seeds for ethanol fermentation, The ethanol-containing liquid phase can be transferred to the distillation and dehydration apparatus to increase the process and ethanol production efficiency.

1 is a flow diagram of a process according to the present invention.
2 is a graph showing sugar concentration and ethanol concentration according to fermentation time.

The present invention relates to a method for producing bioethanol using a single-watered juice as a medium for seed culture for ethanol production fermentation process.

More specifically, (a) preparing a medium by concentrating the juice of a single water sample; (b) culturing the seed culture by adding yeast to the culture medium to obtain a culture solution; (c) separating the culture solution into a liquid phase and a solid phase, recovering the cells obtained from the solid phase, and introducing the cells into a fermentation tank to obtain a fermentation broth containing ethanol; And (d) subjecting the fermentation broth to distillation and dehydration to produce ethanol; .

Here, the term "single-step water juice" means juice extracted from a single water.

The step (a) may include crushing and pressing the single water to form a single water juice; Filtering the single-step water juice through a 0.05 mm to 0.2 mm filter net; And concentrating the filtered juice solution under reduced pressure at a pressure of 1.3 to 133 Pa to increase the sugar content to 50 to 70 Brix at 40 to 70 캜; .

The step (b) may further include diluting the concentrated aqueous solution of aqua regia; And adding 0.1 to 1% (v / v) yeast to the diluted, watery juice, and culturing under aerobic conditions for 10 to 24 hours to obtain a culture solution containing the cells; And a control unit.

In particular, the pH of the culture medium is adjusted to 4.5 to 5.5 at the time of culturing, the fermentation temperature is maintained at 27 to 35 ° C, and the expiration condition is characterized by a flow rate of 0.3 vvm to 1.0 vvm. At this time, the filtered oxygen can be supplied.

At this time, the yeast was Saccharomyces cerevisiae , Kluyveromyces marxianus or Zymomonas mobilis , and one example is Saccharomyces cerevisiae CHY1011 was used.

On the other hand, in the step (c), the cells can be introduced into a fermentation tank containing a saccharified liquid.

In particular, the saccharified liquid according to the present invention is characterized in that it is extracted from fibrous biomass. Here, fiber-based biomass may mean a second-generation bio-ethanol raw material.

In addition, the thus-cultivated seeds are characterized in being fermented by the anaerobic tank during the main culture for ethanol production, that is, during the fermentation using the fibrous glycosylated liquid as a substrate.

Thus, the present invention can increase the ethanol production efficiency by combining the first-generation bioethanol feedstock with the second-generation bioethanol production process.

Here, the first generation bioethanol raw material refers to biomass including mainly carbohydrates (sugar cane, beet), starchy maize (corn, potato, sweet potato, etc.), and the second generation bioethanol raw material refers to lignocellulose (wood, rice straw, waste paper, etc.) containing lignocelluloses.

In addition, in step (d) of the present invention, the liquid phase separated in step (c) may be further transferred to a distillation and dehydration process to produce ethanol.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are intended to illustrate the contents of the present invention, but the scope of the present invention is not limited to the following examples. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art.

< Example >

Example  One. Singular number The juice  Concentrate and prepare the medium

Rural Development Administration After separating the seeds and leaves of Sorghum bicolor (L. ) Moench harvested from the Bio Energy Crop Center of the National Institute of Food Science and Technology, only a small number of juices are squeezed out, and the single juice juice is filtered with a 0.05 mm to 0.2 mm Respectively. After filtration, the juice was concentrated and stored at a low temperature of 5 ° C or lower.

At the time of concentration, the mixture was concentrated under reduced pressure at 50 to 70 brix at 40 to 70 DEG C so that the nutrients present in the sample would not be destroyed. When used as a medium, it was diluted according to the concentration of culture.

Example  2. A step of culturing the seed culture by adding yeast to the medium to obtain a culture solution

The concentrated medium was diluted according to the culture concentration. Yeast was added to the diluted culture medium at 0.1 to 1% (v / v) and cultured under aerobic conditions for 10 to 24 hours to obtain a culture solution containing the cells.

The yeast used was CHY1011 ( Saccharomyces cerevisiae CHY1011) in Saccharomyces cerevisiae , which was distributed from Changhae Research Institute.

Culturing conditions of yeast fermentation were aerated with 0.5 vvm flow rate, pH was adjusted to 4.5 to 5.5, and temperature was maintained at 27 to 35 ° C.

Example  3 &lt; / RTI &gt; ethanol and a step of producing bioethanol

The culture broth containing the cells was separated into a liquid phase and a solid phase using a centrifuge. First, the cells obtained from the solid phase were recovered and used as seeds of ethanol fermentation using a saccharified liquid extracted from fibrous biomass as a substrate.

At this time, the fermentation conditions were adjusted to anaerobic conditions at 33 DEG C for 24 hours and the pH was adjusted to 4.5 to 5.5.

After the fermentation was completed, bioethanol was produced by distillation and dehydration. In addition, since the liquid phase obtained by centrifuging the culture liquid contained ethanol, it was distilled to produce bioethanol.

< Comparative Example >

The yield of ethanol fermentation was investigated using a single water juice medium containing YP (yeast extract, peptone). yeast extract and peptone were all products of BD Difco ^TM . As a fermentation medium, a concentrated aqueous solution of single water was used, and as a control, a single water solution containing YP (yeast extract 1%, peptone 1%) medium was used. The fermentation strain was Saccharomyces cerevisiae CHY1011 was used. The fermentation conditions were a fermentation temperature of 33 °, pH 5.0, 150 rpm, and an oxygen supply of 0.5 vvm for aerobic culture. For ethanol fermentation, 5L fermentation tank was used and the effective volume of culture was 2L. The results of the comparison of sugar consumption and ethanol production characteristics according to fermentation time are shown in Fig.

The fermentation yields were 85% ± 2% when fermented for 24 hrs, and 85% ± 2% when fermented by adding YP medium to single fermented juices.

Respectively. As shown in FIG. 2, since there is no difference between the case where only the single water juice liquid is added and the case where the YP medium is added as the fermentation medium, when only the single water juice is used as the medium, it is not necessary to add additional inorganic components, .

sample Concentration per sugar (g / L) Total N (%) pH Salinity EC (%) Glucose Fructose Sucrose Total Singular number
Juice 11.4 14.3 100.0 125.6 0.05 4.96 0.69

ingredient PO ₄ -P NO ₃ -N NH ₄ ⁺ -N mg / L 146.93 109.63 11.19

ingredient Cu Fe Mn Zn P Ca Mg Na K mg / kg 0.59 5.51 0.95 0.98 181.84 237.46 137.33 13.28 4429.07

Table 1 shows the composition including the sugar component of the single water solution, and the ion component and the mineral content of the single water solution are shown in Table 2 and Table 3, respectively. It was confirmed that the constituents of the single water juice shown in Tables 1 to 3 were used as nutrients for fermentation of yeast, so that even if no additional nitrogen source was supplied through YP, it did not affect fermentation, Were found to be suitable as medium for this seed culture.

Claims (9)

(a) preparing a medium by concentrating the juice of a single water sample;
(b) culturing the seed culture by adding yeast to the culture medium to obtain a culture solution;
(c) separating the culture solution into a liquid phase and a solid phase, recovering the cells obtained from the solid phase, and introducing the cells into a fermentation tank to obtain a fermentation broth containing ethanol; And
(d) subjecting the fermentation broth to a distillation and dehydration process to produce ethanol; &Lt; / RTI &gt; The method according to claim 1,
Wherein the step (a) comprises crushing and pressing the single water to obtain a single water juice;
Filtering the single-step water juice through a 0.05 mm to 0.2 mm filter net; And
Concentrating the filtered juice solution at a reduced pressure of 1.3 to 133 Pa to raise the sugar content to 50 to 70 Brix at 40 to 70 캜; &Lt; / RTI &gt; The method according to claim 1,
(B) diluting the concentrated medium; And
Adding 0.5 to 1% (v / v) yeast to the diluted culture medium and culturing under aerobic conditions for 10 to 24 hours to obtain a culture solution containing cells; &Lt; / RTI &gt; The method of claim 3,
Wherein the pH of the culture medium is adjusted to 4.5 to 5.5 and the temperature is maintained at 27 to 35 &lt; 0 &gt; C. The method of claim 3,
Wherein the aerobic conditions are such that air is supplied at a flow rate of 0.3 vvm to 1.0 vvm to supply oxygen. The method according to claim 1,
The yeastSaccharomyces cerevisiae , Kluyveromyces marxianus orZymomonas mobilis Wherein the bioethanol is produced by a method comprising the steps of: The method according to claim 1,
Wherein the fermentation tank comprises a saccharified liquid. 8. The method of claim 7,
Wherein the saccharified liquid is extracted from fibrous biomass. The method according to claim 1,
Wherein the step (d) further comprises feeding the liquid phase separated in the step (c) to a distillation and dehydration process to produce ethanol.

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