KR20140090863A - Method for preparing red panax ginseng extract with enhanced nonpolar ginsenosides contents - Google Patents

Abstract

The present invention relates to a method for producing a red ginseng extract with an increased non-polar ginsenoside content. More specifically, by using a method for heating and extracting red ginseng in water to remove a water-soluble material including polar ginsenoside and extracting and concentrating remaining residues with alcohol, a red ginseng extract with an improved function can be produced while remarkably increasing the content of non-polar ginsenoside. A method for extracting alcohol using red ginseng water-extracted residues is used to produce a red ginseng concentrate containing a large quantity of non-polar ginsenoside exhibiting various physiological functions. Thereby, the method can be usefully utilized to develop a product which can enhance a particular functionality of a red ginseng product.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a method for preparing red ginseng extract having enhanced nonpolar ginsenoside content,

The present invention relates to a method for preparing red ginseng extract having a high content of nonpolar ginsenosides and improved properties.

From ancient times, red ginseng has been used by many people because of its excellent efficacy such as fatigue recovery, immunity enhancement, and improvement of blood circulation. Especially, saponin, which is a major component of red ginseng, is a component called ginsenoside which is the main pharmacological effect of various active ingredients of ginseng. Recently, according to the development of separation and analytical techniques, 30 chemical structures of ginseng saponin have been identified, Saponin pharmacological actions known as the present study are reported to have various pharmacological actions such as antitumor action, anti-fatigue action, mental stabilization action, anti-inflammatory action and aging-inhibiting action.

Ginseng mainly contains only polar ginsenoside, while red ginsex contains polar ginsenosides and trace ginsenosides in trace amounts. Typical non-polar ginsenosides of red ginseng include ginsenoside Rh1, ginsenoside Rh2, ginsenoside Rg2, ginsenoside Rg3, ginsenoside Rg5, ginsenoside Rk1, and the like. Nonpolar ginsenosides often exhibit superior pharmacological activity than polar ginsenosides in general. For example, ginsenoside Rg3, ginsenoside Rk1 or ginsenoside Rg5, which are nonpolar ginsenosides, are known to have excellent functionality among the active ingredients of red ginseng. In particular, ginsenoside Rg3 is known to inhibit cancer cell metastasis, Activity, antithrombotic action, and the like. In addition, ginsenoside Rg5 is known to have an anticancer effect, and the anticancer effect, antioxidative effect and liver protection effect of ginsenoside Rh2 and the neuroprotective effect of ginsenoside Rg2 have been proved.

However, since non-polar ginsenoside exists in an extremely small amount in red ginseng, it is necessary to develop a technique to increase the elution amount of nonpolar ginsenoside in order to produce a product having increased physiological activity in vivo.

It is an object of the present invention to provide a method for producing red ginseng extract containing a high amount of nonpolar ginsenosides.

In order to achieve the above object,

1) obtaining a residue of red ginseng water extract extracted from water with red ginseng; And

2) extracting the residue with an alcohol having 1 to 4 carbon atoms, wherein the non-polar ginsenoside content is increased.

In addition, the present invention provides a method for preparing red ginseng extract having increased non-polar ginsenoside content, comprising extracting red ginseng with water and extracting the remaining residue with an alcohol having 1 to 4 carbon atoms.

The raw material used in the present invention is red ginseng, and the term "red ginseng" means a ginseng prepared by heating ginseng through steam or sun-drying, preferably steam.

The red ginseng can be directly grown or commercially available, and is not particularly limited. For example, red ginseng or fusiform body can be used, and red ginseng can be used in both the United States, Central America and the United States.

In a preferred embodiment of the present invention, the step 1) is a step for removing water-soluble ginsenosides and hydrophilic components in red ginseng. The extraction solvent may be a water-soluble ginsenoside It is preferable to use distilled water.

At this time, the extraction temperature is preferably 80-100 ° C, preferably 85-90 ° C, but is not limited thereto. When the temperature is lower than 80 ° C., the water-soluble materials have a lowered efficiency. When the temperature is higher than 100 ° C., the non-polar ginsenoside components to be extracted are decomposed to lower the extraction yield.

The extraction time of the step 1) may be generally performed for a short time, may be 1 to 10 hours, and is preferably 8 hours, but is not limited thereto. When the extraction time is less than 1 hour, it is difficult to extract a sufficient amount of water-soluble substances from the red ginseng. If the extraction time exceeds 10 hours, the extraction yield is not increased with the increase of the extraction time.

In a preferred embodiment of the present invention, the step 2) is to increase the content of non-polar ginsenosides in the red ginseng extract by extracting the polar ginsenosides obtained in step 1) and the red ginseng residues from which the water-soluble substances have been removed with alcohol . The extraction method is preferably a reflux extraction method, but is not limited thereto. The suitable concentration of the alcohol is 30 to 70% (v / v), preferably 40 to 50%, but is not limited thereto. When the concentration of alcohol is less than 30%, the amount of nonpolar ginsenoside increases very much. When the concentration of alcohol is more than 70%, the amount of nonpolar ginsenoside increases remarkably.

The alcohol is preferably ethanol or methanol, but it is possible to increase the content of nonpolar ginsenosides.

The extraction temperature of step 2) is preferably 60 to 80 ° C, more preferably 63 to 70 ° C, but is not limited thereto.

The non-polar ginsenoside of the present invention is characterized in that ginsenoside Rg3, ginsenoside Rg5, ginsenoside Rg6, ginsenoside Rh4, ginsenoside Rk1, ginsenoside Rk3, And Senocide F4, and more preferably at least one selected from the group consisting of ginsenoside Rg3, ginsenoside Rg5 and ginsenoside Rk1, but is not limited thereto.

In a preferred embodiment of the present invention, after the step 2), the step of concentrating the red ginseng extract obtained in step 2) may further be carried out, and preferably the decompression concentration may be used. At this time, it is preferable to conduct the decompression concentration at least twice.

The reduced-

1) concentrating red ginseng extract to a primary volume of 1/2 to 1/10 of the original volume and cooling it at room temperature; And

2) removing the precipitate of the concentrate obtained in the step 1) and concentrating the concentrate under a reduced pressure to prepare a red ginseng concentrate. By this method, the problem of the property that non-polar substances which are not soluble in water are aggregated as the solvent polarity changes from alcohol to water in the concentration process after cooling and filtering the alcohol extract can be solved.

In the above-mentioned reduced-pressure concentration method, the first reduced pressure and second reduced pressure are preferably carried out at 50 to 70 ° C, more preferably 55 to 65 ° C, but are not limited thereto. In addition, it is preferable to remove the precipitate of the first reduced pressure concentrate after cooling at room temperature after the first reduced pressure concentration and centrifugation or filtration.

In the red ginseng extract having an increased nonpolar ginsenoside content finally obtained by the method of the present invention as described above, the nonpolar ginsenoside may be contained in an amount of 85 to 95% by weight of the total ginsenosides. In addition, the non-polar ginsenoside content of the red ginseng extract may be 6 to 18 times higher than that of the red ginseng water extract prepared in the step 1) of the above production method.

In a specific example of the present invention, in order to prepare a red ginseng extract having a high content of nonpolar ginsenoside, 5 to 10, preferably 7-fold, water was added to the red ginseng raw material, After removing the red ginseng water extract obtained by centrifugation, 10 times of 30-70% ethanol was added to the remaining residue, and the mixture was allowed to stand for reflux. The obtained red ginseng extract is concentrated under reduced pressure at 65 캜, concentrated to 1/5 level of the extract, and cooled to less than 25 캜. The cooled concentrate can then be centrifuged or filtered to remove insoluble precipitates and further subjected to a second vacuum concentration to produce the red ginseng extract (see Figures 1 and 2).

The red ginseng extract of the present invention produced by the above method showed that the dried residue of the red ginseng water extract contained a high content of nonpolar ginsenoside (see Table 1), and the red ginseng was extracted with water The remaining residue is re-extracted with alcohol to determine the content of nonpolar ginsenosides such as ginsenoside Rg3, ginsenoside Rg5, ginsenoside Rg6, ginsenoside Rh4, ginsenoside Rk1, ginsenoside Rk3 and ginsenoside F4 Was remarkably higher than that of the conventional red ginseng water or alcohol alone by 6 to 18 times (see Table 2). In addition, when the ethanol extract was used to extract the dried residue from red ginseng water extraction, the yields and the content of ginsenosides were different according to the concentration of ethanol used as alcohol, , The content of nonpolar ginsenoside increased with increasing ethanol concentration, and 30 ~ 70% ethanol was found to be a suitable concentration. Especially, when the concentration was above 40%, the content of nonpolar ginsenoside was effectively increased 4 and Table 2).

In addition, in order to prevent the aggregation phenomenon of the non-polar substances occurring in the case of alcoholic extracts and to improve the properties of the extracts, the extract is first subjected to an additional step of filtering the extracts under reduced pressure and then removing the insoluble precipitates, The properties of the non-polar ginsenosides were not affected (see Table 3), and the properties were improved in terms of color, physical properties, homogeneity and stability of the red ginseng extract (Figs. 5 to 7 Reference).

Therefore, the production method of the present invention as described above can be effectively used for preparing red ginseng extract having improved properties while containing non-polar ginsenosides.

In addition, the present invention provides red ginseng extract having increased nonpolar ginsenoside content prepared by the above method.

The red ginseng extract may contain the nonpolar ginsenosides in the extract in an amount of 85 to 95% by weight of the total ginsenosides. In addition, the non-polar ginsenoside content of the red ginseng extract may be 6 to 18 times as high as that of the red ginseng water extract prepared in the step 1).

The red ginseng extract is a red ginseng extract having improved color, physical properties, homogeneity and stability of the extract and improved properties by the production method of the present invention.

The non-polar ginsenosides of the present invention have increased physiologically active functions such as dementia, anticancer effect, hair growth promotion, and blood pressure reduction, compared with the red ginseng extract prepared by the conventional extraction method, , So that it can be usefully used for red ginseng products that have enhanced the efficacy of non-polar ginsenoside.

The process for preparing red ginseng extract according to the present invention relates to a process for preparing red ginseng extract containing a high amount of nonpolar ginsenosides, which comprises extracting and removing a polar organic substance and a polar ginsenoside by heating and removing the nonpolar ginsenoside Since the senoside is extracted with a non-polar solvent alcohol, it is possible to produce a red ginseng concentrate having a high content of non-polar ginsenoside and excellent in utilization of materials in that the red ginseng water extract is used and remaining residue is used .

1 is a view showing a manufacturing process of the present invention.
FIG. 2 is a diagram illustrating a comparison between a manufacturing process of the present invention and a conventional manufacturing process.
FIG. 3 is a graph showing the production yields of ethanol extracts of alcoholic extracts of red ginseng water-extracted residue. FIG.
4 is a graph showing the content of ginsenoside-Rg3 by ethanol concentration at the time of alcohol extraction with dried red ginseng water-extracted residue.
FIG. 5 is a view showing the characteristics of the red ginseng extract according to the concentration method.
6 is a graph showing changes in the properties of red ginseng extract prepared according to Comparative Example 2. FIG.
FIG. 7 is a graph showing changes in properties of red ginseng extract of the present invention. FIG.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following examples and experimental examples are intended to further illustrate the present invention, and the scope of the present invention is not limited by these examples and experimental examples.

< Example  1> Non-polar Gin Senocide High-content  Production of red ginseng extract

Red ginseng ingredients were Korean ginseng 6 - year - old red ginseng. Specifically, water (7 w / w) was added to red ginseng and extracted three times at 87 ° C for 8 hours. The extract was discarded and the extracted residue was dried and pulverized to 20 mesh or less. Then, the dried residue was placed in a nonwoven fabric, and 10 to 10% (w / w) of 30 to 70% ethanol was added thereto, followed by reflux extraction. The extract was concentrated under reduced pressure to a level of 1/5 of the extract at a bath temperature of 65 ° C using a concentrator, and the concentrate was cooled to less than 25 ° C. The cooled concentrate was centrifuged at 6,000 rpm to remove insoluble precipitates, and the supernatant was concentrated under reduced pressure at a bath temperature of 65 DEG C to prepare a concentrate. At this time, in order to calculate accurate production yield and ginsenoside content, the solid content of the concentrate was corrected to 64% to prepare a red ginseng concentrate (FIG. 1).

< Comparative Example  1> Preparation of Red Ginseng Water Extract and Red Ginseng Ethanol Extract Using Conventional Concentration Method

According to the conventional method of extracting red ginseng, the method of extracting with water is 7 times at 87 ° C for 8 hours, and the method of extracting with ethanol (20 to 55% (v / v)) is 65 ° C for 8 hours Respectively. After the extraction, the extracts were separated and the extracts were cooled to 25 ° C or lower, centrifuged at 6,000 rpm to remove precipitates, and concentrated under reduced pressure at a bath temperature of 65 ° C using a concentrator to prepare a red ginseng concentrate having a solid content of 64% Respectively.

< Comparative Example  2> Non-polarity using the conventional concentration method Gin Senocide High-content  Production of red ginseng extract

In order to compare the effect according to the concentration method, the red ginseng extract was prepared according to the method of Example 1, and then the following conventional methods were used for concentration. That is, the extract was cooled to 25 ° C or lower, centrifuged at 6,000 rpm to remove precipitates, and concentrated under reduced pressure at a bath temperature of 65 ° C using a concentrator to prepare a red ginseng concentrate having a solid content of 64% (FIG. 2).

< Experimental Example  1> Red ginseng concentrate Gin Senocide  Content check

<1-1> Red ginseng extract solvent Residue  Dry Gin Senocide  Content comparison

The content of ginsenoside contained in the red ginseng extract residue and the 55% ethanol extract residue obtained in Comparative Example 1 was measured to confirm the contents of the ginsenosides in the dried residue of the ginseng extract. Specifically, 2 g of the red ginseng concentrate finally obtained in Example 1 was dissolved in 50 ml of distilled water, and the solution was filtered through a 0.45 탆 syringe filter to prepare a test sample. Rf, Rf, Rh1, Rg2s, Rb1, Rc, Rb2, Rd, Rg3s, Rg3r, Rg6, Rk3, F4, Rh4 (Rg1, , Rk1 and Rg5 were measured. The standard solutions were purchased from Chromadex and the concentrations of the ginsenosides Rg2s, Rg6, Rk3, F4, Rh4, Rk1, and Rg5 The standard solution was purchased from Ambo Laboratories. The standard solution was subjected to high-performance liquid chromatography to prepare a standard calibration curve, and the ginsenoside content of the test sample was measured.

The analysis conditions of the UPLC-UVD (Acquity UPLC system, Waters) of the ginsenoside component were as follows. The solvent B ratio was changed from 15% to 15% (10.5 min) using H ₂ O (A) and CH ₃ CN (B) (30 minutes), 30% (14.5 minutes), 32% (15.5 minutes), 38% (18.5 minutes), 43% (24 minutes), 55% (27 minutes), 55% % (38 min), and finally to 15% (38.1 min). The column temperature was set at 40 ° C, the flow rate was set at 0.6 ml per minute, and the UV wavelength was set at 203 nm.

As a result, the content of water soluble polar ginsenoside (Rg1, Re, Rf, Rb1, Rb2, Rc, Rd and Rf) in red ginseng extract was lower than that of 55% The content of the nonpolar ginsenosides (Rg3, Rg6, Rk3 , F4, Rh4, Rk1, Rg5) was measured to be high and it was confirmed that the residue obtained by distilled water extraction contained a large amount of water-insoluble nonpolar ginsenosides One).

Extraction solvent Ginsenoside content [mg / g] Rf Rh1 Rg2s Rb1 Rc Rb2 Rd Rg3s Rg3r Rg6 Rk3 F4 Rh4 Rk1 Rg5 Distilled water 0.09 0.18 0.18 0.18 0.12 0.07 0.07 2.92 2.54 0.28 0.45 0.70 0.98 2.01 3.06 0.04 0.18 0.17 0.19 0.11 0.07 0.07 1.55 1.78 0.10 0.26 0.32 0.64 1.34 2.09 0.06 0.17 0.11 ND ND ND ND 2.19 2.08 0.18 0.30 0.45 0.72 1.57 2.60 55% ethanol ND 0.07 0.06 0.23 ND ND ND 0.81 0.57 0.08 0.11 0.18 0.23 0.52 0.66 ND 0.07 0.08 0.24 ND ND 0.02 0.86 0.84 0.07 0.08 0.20 0.26 0.58 0.79 ND 0.05 0.03 0.20 ND ND ND 0.71 0.45 0.05 0.06 0.15 0.20 0.44 0.60

&Lt; 1-2 > The red ginseng water extract Residue  Ethanol extract and Control Of red ginseng water extract or red ginseng alcohol extract Gin Senocide  Content comparison

The ginsenoside content of the red ginseng extracts prepared in Example 1 and Comparative Example 1 was measured and the content of ginsenosides of the red ginseng extract obtained by extracting the red ginseng water extract residue dried with ethanol was compared with that of the conventional red ginseng water extract or alcohol extract Respectively.

As a result, in the preparation of the red ginseng extract according to the present invention, there was no significant difference in the production yield depending on the concentration of ethanol (FIG. 3), but as the concentration of ethanol increased, one of the nonpolar ginsenosides The content of ginsenoside-Rg3 was increased and the content of ginsenoside Rg3 was maintained at higher than 40% (Fig. 4). As a result of comparing the content of ginsenosides of red ginseng extract according to the present invention with that of conventional red ginseng water extract or ethanol extract, it was found that the red ginseng extract obtained by extracting the red ginseng water extract residue of the present invention with ethanol, The content of ginsenoside was found to be about 6 to 18 times higher, and it was confirmed that ginsenosides-Rg3, -Rg5 and Rk1 constitute more than 70% of the total ginsenosides.

In order to establish the detailed extraction conditions, the content of ginsenosides according to the concentration of ethanol was measured. As the ethanol concentration increased, the content of nonpolar ginsenoside increased. Especially, the concentration of ethanol increased from 30% Of the non-polar ginsenosides were eluted, with maximum concentrations at 50% ethanol concentration and similar contents at the concentrations above (Table 2).

Gin Senocide Content [mg / g] (based on 64% solids) Control The ginseng extract of the present invention (by ethanol concentration) Red ginseng water extract Red Ginseng Alcohol Extract 20% 30% 40% 50% 60% 70% Rg1 1.08 10.18 Non-detection Non-detection Non-detection Non-detection Non-detection Non-detection Re 1.52 10.22 Non-detection Non-detection Non-detection Non-detection Non-detection Non-detection Rf 1.59 3.31 0.53 0.99 1.03 1.08 0.91 0.94 Rh1 1.10 0.63 1.13 2.27 1.92 2.52 2.20 2.51 Rg2s 1.75 1.67 1.11 2.14 1.85 2.44 1.92 2.28 Rb1 6.59 30.87 1.26 2.46 2.44 3.12 3.06 2.93 Rc 2.87 12.78 0.33 0.63 0.62 0.82 0.73 0.79 Ra1 1.69 6.37 0.12 0.24 0.24 0.31 0.30 0.29 Rb2 2.61 11.43 0.35 0.70 0.67 0.89 0.85 0.85 Rd 1.07 2.73 0.25 0.59 0.61 0.83 0.81 0.83 Rg6 0.67 0.35 0.57 2.41 2.59 2.76 2.64 2.70 Rk3 0.45 0.30 0.76 3.22 3.45 3.69 3.52 3.61 F4 1.20 0.57 1.25 5.32 5.71 6.10 5.82 5.96 Rh4 0.83 0.62 1.76 7.48 8.03 8.58 8.19 8.39 Rg3s 3.21 0.97 4.12 17.50 18.80 20.07 19.17 19.63 Rg3r 1.03 0.67 0.70 4.05 12.66 15.07 15.04 16.09 Rk1 1.76 1.00 4.41 18.72 20.11 21.48 20.51 21.00 Rg5 2.06 1.04 5.27 22.39 24.05 25.68 24.52 25.12 The sum of Rg3, Rk1 and Rg5 8.06 3.68 14.5 62.66 75.62 82.30 79.24 81.84 Nonpolar
Gin Senocide * 11.21 5.52 18.83 81.08 95.40 103.42 99.40 102.51 gun 33.08 95.71 23.89 91.10 104.78 115.42 110.19 113.93

The sum of ginsenosides-Rg3, -Rg5, -Rg6, -Rh4, -Rk1, -Rk3 and -F4

&Lt; 1-3 > Gin Senocide  Content check

In order to compare the ginsenoside content of the red ginseng extract according to the concentration method of the present invention and the conventional concentration method in the manufacturing process, the ginsenoside content of the red ginseng extract prepared in each of Example 1 and Comparative Example 2 was measured Respectively.

As a result, there was almost no difference in the content of ginsenosides according to the concentration method (Table 3).

Gin Senocide Content [mg / g] (based on 64% solids) Example 1 Comparative Example 2 Rg1 Non-detection Non-detection Re Non-detection Non-detection Rf 0.67 0.75 Rh1 02.46 2.60 Rg2s 2.19 2.26 Rb1 2.51 2.70 Rc 0.67 0.75 Ra1 0.25 0.27 Rb2 1.29 1.02 Rd 1.17 1.16 Rg6 2.56 2.47 Rk3 3.52 3.33 F4 5.51 5.71 Rh4 8.28 8.22 Rg3s 19.31 20.02 Rg3r 16.47 13.11 Rk1 18.04 18.60 Rg5 15.17 14.25 The sum of Rg3, Rk1, Rg5 68.99 65.98 Nonpolar ginsenoside * 88.86 85.71 gun 99.99 93.32

The sum of ginsenosides-Rg3, -Rg5, -Rg6, -Rh4, -Rk1, -Rk3 and -F4

< Experimental Example  2> Identification of Properties of Red Ginseng Extracts by Concentration Method

The properties of the red ginseng extracts prepared in Example 1 and Comparative Example 2 were visually confirmed.

As a result, problems in terms of color, physical properties and homogeneity were found in the properties of the red ginseng extract of Comparative Example 2 using the conventional concentration method. On the other hand, the red ginseng extract of the present invention prepared in Example 1 showed improved properties. In the case of Comparative Example 2, it was confirmed that when the polarity of the solvent was changed from ethanol to water during the concentration process after cooling or filtering the ethanol extract, it was confirmed that a large amount of non-polar substances which are not soluble in water are aggregated, In the case of the red ginseng extract of Example 1 prepared by the concentration method of the present invention (Fig. 5). Therefore, it was possible to solve the property problem of extracting the water extraction residue with alcohol by the additional process during concentration.

< Experimental Example  3> Stability test

In order to compare the stability of the red ginseng extracts prepared in Example 1 and Comparative Example 2, each red ginseng extract was stored at 40 ° C and 75% for 2 weeks and then observed.

As a result, it was confirmed that the red ginseng extract of Comparative Example 2 had a fat-soluble component aggregated and precipitated after two weeks, resulting in layer separation (Fig. 6), but the red ginseng extract of the present invention showed no change in properties even after 2 weeks (Fig. 7).

Claims (14)

1) obtaining a residue of red ginseng water extract extracted from water with red ginseng; And
2) Extracting the residue with an alcohol having 1 to 4 carbon atoms, wherein the non-polar ginsenoside content is increased. The method according to claim 1,
Wherein the step (1) is carried out by heating at 80 to 100 ° C, wherein the nonpolar ginsenoside content is increased. Extracting red ginseng with water, and extracting the remaining residue with an alcohol having 1 to 4 carbon atoms, wherein the non-polar ginsenoside content is increased. The method according to any one of claims 1 and 3,
Wherein the alcohol is an alcohol of 30 to 70% (v / v), wherein the non-polar ginsenoside content is increased. The method according to any one of claims 1 and 3,
Wherein the alcohol is ethanol or methanol, wherein the non-polar ginsenoside content is increased. The method according to any one of claims 1 and 3,
Wherein the step of extracting with alcohol is reflux-extraction, wherein the non-polar ginsenoside content is increased. The method according to any one of claims 1 and 3,
The nonpolar ginsenoside is any one or more selected from the group consisting of ginsenoside Rg3, ginsenoside Rg5, ginsenoside Rg6, ginsenoside Rh4, ginsenoside Rk1, ginsenoside Rk3 and ginsenoside F4 A method for preparing red ginseng extract having increased non - polar ginsenoside content. The method according to any one of claims 1 and 3,
Wherein the step of extracting the red ginseng extract further comprises the step of concentrating the red ginseng extract obtained in the step of extracting with the alcohol at a reduced pressure, wherein the non-polar ginsenoside content is increased. The method of claim 8,
Wherein the reduced-pressure concentration is repeated at least twice, wherein the non-polar ginsenoside content is increased. The method of claim 8,
The reduced-
1) concentrating the red ginseng extract at a first reduced pressure of 1/2 to 1/10 the original volume; And
2) removing the precipitate of the concentrate obtained in the step 1), and concentrating the second concentration to obtain a red ginseng concentrate, wherein the non-polar ginsenoside content is increased. The method of claim 10,
Wherein the concentration of the non-polar ginsenosides is increased at the first vacuum concentration and the second vacuum concentration at 50-70 &lt; 0 &gt; C. The method according to any one of claims 1 and 3,
Wherein the non-polar ginsenoside content is increased in the non-polar ginsenoside content, the non-polar ginsenoside content is 85 to 95 wt% of the total ginsenoside content. The method according to any one of claims 1 and 3,
Wherein the nonpolar ginsenoside content of the red ginseng extract is increased by 6 to 18 times the non-polar ginsenoside content as compared to the red ginseng water extract. A ginseng extract having increased non-polar ginsenoside content produced by the method according to any one of claims 1 and 3.

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